Lag0008326 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0008326
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionGag protease polyprotein
Locationchr9: 17684438 .. 17685101 (+)
RNA-Seq ExpressionLag0008326
SyntenyLag0008326
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGATGCCCCGAGGCACAACAGGTGTCGTGTGCAGTATTTATGTTAAGGGGCGATACCTTGTTGTGGTGGAGGTCGGCTAAGAGATCCATCGATGTAAGTGTTGGACCGATCACTTGGTTGCAGTTCAGGAGGCATTCTTCTAGAAATATTACTCAGCCATTAGAAGATACAGAAATGAGAAGGAGTTTCTAACCTTGATGCAAGGAGAGAAGTCAGTAGAAGAATACGAGCTGGGGTTCACCCGATTGTCCCGAAGAAGACAAAGGGGTTCATCTTGGGCCTCAGAGATGACGTCTAGAGGGTTGTTAAAGCTCTTGGCCCAGTAGATTACGAAGTGGCCCTTCGAGCGGCCACCTTTATGGGCATGCCAATTGTCAATGCAACTCCAGTAGCCAAAGAGTCGGAGCCCAACGCAAGACAGAAGAGAAAACACGAGCAGACAACTGCTAACTTCCAACGATCTCAACACTCTTCTGAAGGTTCAAGACAGAAAACTCAGCATGGCAAACAAGAGGGTGATGGTAACGATAAACCAAAGTGCAACTCTTGTGGAAGACATCATTGGGGTCAGTGCTTGGAAGGGAAAGACGTGCGTTTCAAATGTCACAAGGAAGGGCATATGGCAAATAACTGCCCTCAGAAGAAAGTGGGAGGCTCGTGA

mRNA sequence

ATGGGATGCCCCGAGGCACAACAGGTGTCGTGTGCAGTATTTATGTTAAGGGGCGATACCTTGTTGTGGTGGAGGTCGGCTAAGAGATCCATCGATAGGGTTGTTAAAGCTCTTGGCCCAGTAGATTACGAAGTGGCCCTTCGAGCGGCCACCTTTATGGGCATGCCAATTGTCAATGCAACTCCAGTAGCCAAAGAGTCGGAGCCCAACGCAAGACAGAAGAGAAAACACGAGCAGACAACTGCTAACTTCCAACGATCTCAACACTCTTCTGAAGGTTCAAGACAGAAAACTCAGCATGGCAAACAAGAGGGTGATGGTAACGATAAACCAAAGTGCAACTCTTGTGGAAGACATCATTGGGGTCAGTGCTTGGAAGGGAAAGACGTGCGTTTCAAATGTCACAAGGAAGGGCATATGGCAAATAACTGCCCTCAGAAGAAAGTGGGAGGCTCGTGA

Coding sequence (CDS)

ATGGGATGCCCCGAGGCACAACAGGTGTCGTGTGCAGTATTTATGTTAAGGGGCGATACCTTGTTGTGGTGGAGGTCGGCTAAGAGATCCATCGATAGGGTTGTTAAAGCTCTTGGCCCAGTAGATTACGAAGTGGCCCTTCGAGCGGCCACCTTTATGGGCATGCCAATTGTCAATGCAACTCCAGTAGCCAAAGAGTCGGAGCCCAACGCAAGACAGAAGAGAAAACACGAGCAGACAACTGCTAACTTCCAACGATCTCAACACTCTTCTGAAGGTTCAAGACAGAAAACTCAGCATGGCAAACAAGAGGGTGATGGTAACGATAAACCAAAGTGCAACTCTTGTGGAAGACATCATTGGGGTCAGTGCTTGGAAGGGAAAGACGTGCGTTTCAAATGTCACAAGGAAGGGCATATGGCAAATAACTGCCCTCAGAAGAAAGTGGGAGGCTCGTGA

Protein sequence

MGCPEAQQVSCAVFMLRGDTLLWWRSAKRSIDRVVKALGPVDYEVALRAATFMGMPIVNATPVAKESEPNARQKRKHEQTTANFQRSQHSSEGSRQKTQHGKQEGDGNDKPKCNSCGRHHWGQCLEGKDVRFKCHKEGHMANNCPQKKVGGS
Homology
BLAST of Lag0008326 vs. NCBI nr
Match: XP_022934554.1 (uncharacterized protein LOC111441700 [Cucurbita moschata])

HSP 1 Score: 73.9 bits (180), Expect = 1.3e-09
Identity = 59/211 (27.96%), Postives = 80/211 (37.91%), Query Frame = 0

Query: 3   CPEAQQVSCAVFMLRGDTLLWWRSAK---------------------------------- 62
           CPEA +V CA+FML  D  LWW++ +                                  
Sbjct: 15  CPEAHRVECAMFMLHDDAELWWQATRDLISPEGGVVSWVEFKDVFSKQYYPEDVQLRKQQ 74

Query: 63  -----------------------------RSIDRVVKALGPVDYEVALRAATFMGMPIVN 122
                                          I   VKA+ P  Y  ALRAA  M  P  +
Sbjct: 75  KEFSKLKRFAPELVNTDYKTARQFVLGLDTKIRNTVKAISPTTYAAALRAAKAMEGPDSS 134

Query: 123 ATPVAKESEPNARQKRKHEQTTAN---FQRSQHSSEGSR---QKTQHGKQEGDGNDKPKC 145
             P +       RQKR+ +QT  N   F ++Q  S+  R   ++ Q G  E     +PKC
Sbjct: 135 REPPSS----LVRQKRRRDQTDRNELSFPQTQRRSDRQRLDYRREQQG-SENRSESRPKC 194

BLAST of Lag0008326 vs. NCBI nr
Match: KAA0025929.1 (gag protease polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 71.6 bits (174), Expect = 6.5e-09
Identity = 46/159 (28.93%), Postives = 73/159 (45.91%), Query Frame = 0

Query: 1   MGCPEAQQVSCAVFMLRGDTLLWWRSAKRSIDRVVKALGPVDYEVALRAATFMGMPIVNA 60
           + CP+ Q+V CA+FML      WW + +R ++  V  +    ++ +  A  F      + 
Sbjct: 250 LSCPKDQKVQCAIFMLTDRGTAWWETIERMLEGDVGQITWEQFKESFYAKLFS----ASL 309

Query: 61  TPVAKESEPNARQKRKHEQTTANFQRSQHSS-------EGSRQKTQHGKQEGDGNDKPKC 120
               ++   N  Q  +      N Q   +SS       E  R + +  + E     KP C
Sbjct: 310 RDAKRQEFLNLEQDDRTGSMDLNLQERANSSKVASRGGEFFRFQQKPFEAEEAARGKPLC 369

Query: 121 NSCGRHHWGQCLEGKDVRFKCHKEGHMANNCPQKKVGGS 153
            +CG+HH G+CL G    FKC +EGH A+ CP +  GG+
Sbjct: 370 TTCGKHHLGRCLFGTRTCFKCRQEGHTADRCPMRLTGGA 404

BLAST of Lag0008326 vs. NCBI nr
Match: KAA0047194.1 (DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa])

HSP 1 Score: 71.2 bits (173), Expect = 8.4e-09
Identity = 52/186 (27.96%), Postives = 80/186 (43.01%), Query Frame = 0

Query: 1   MGCPEAQQVSCAVFMLRGDTLLWWRSAKR------------------------------- 60
           M CPE Q+V CAVFML      WW + +R                               
Sbjct: 339 MKCPEDQKVQCAVFMLTDRGNAWWETTERMLGGDWNNMTRSLTCYPTEAARADKFVRGLR 398

Query: 61  -SIDRVVKALGPVDYEVALRAATFMGM-PIVNATPVAKESEPNARQKRKHEQTTANFQRS 120
            +I  +V+A  P  +  ALR A  + +    N++  A     + ++++  +Q     QR+
Sbjct: 399 LNIQGLVRAFRPATHADALRLAVDLSLQERANSSKTAGRGSTSGQKRKAEQQPVPVPQRN 458

Query: 121 QHSSEGSRQKTQHGKQEGD-GNDKPKCNSCGRHHWGQCLEGKDVRFKCHKEGHMANNCPQ 153
             S    R+  Q   + G+    KP C +CG+HH G+CL      FKC +EGH A+ CP 
Sbjct: 459 FRSGGAFRRFQQKPFEAGEAARGKPLCTTCGKHHLGRCLFETRTCFKCRQEGHTADRCPL 518

BLAST of Lag0008326 vs. NCBI nr
Match: XP_022932138.1 (uncharacterized protein LOC111438460 [Cucurbita moschata])

HSP 1 Score: 71.2 bits (173), Expect = 8.4e-09
Identity = 57/170 (33.53%), Postives = 72/170 (42.35%), Query Frame = 0

Query: 3   CPEAQQVSCAVFMLRGDTLLWWRSAKRSI-----------------------DRVVKALG 62
           CPEA +V CA FMLR D  LWW++ +  I                       DR    L 
Sbjct: 43  CPEAHRVECATFMLREDAELWWQATRDIISPEGGAVSWVEFKSAFTEEYYTEDRFAPELV 102

Query: 63  PVDYEVALRAATFMGMPIVNATPVAKESEPNARQKRKHEQTTANFQRSQHSSEGS----- 122
             DY  A R    +G+     T + K  E  A          A       SS  +     
Sbjct: 103 NTDYRTARRFV--LGLD----TKIRKTVEAIAPATYAAALRAAKSMEGLDSSHETPSLLV 162

Query: 123 RQKTQHGKQEGDGNDKPKCNSCGRHHWGQCLEGKDVRFKCHKEGHMANNC 145
            QK +H + E   +DKPKCN CG++HWGQCL      F+C KE HMA +C
Sbjct: 163 GQKHRH-EYENKLDDKPKCNKCGKNHWGQCLGHIRACFRCGKEDHMAKDC 205

BLAST of Lag0008326 vs. NCBI nr
Match: KAA0033018.1 (gag protease polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 70.5 bits (171), Expect = 1.4e-08
Identity = 53/167 (31.74%), Postives = 74/167 (44.31%), Query Frame = 0

Query: 1   MGCPEAQQVSCAVFMLRGDTLLWWRSAKRSI--DRVVKALGPVDYEVALRAATFMGMPIV 60
           M CPE Q+V CAVFML      WW + +R +  D+    +   D E  + +   + M   
Sbjct: 221 MKCPEDQKVQCAVFMLTDRGTAWWETTERMLGGDQGDMTVEQYDAEFDMLSHFALEMIAT 280

Query: 61  NATPV------AKESEPNAR-----QKRKHEQTTANFQRSQHSSEGSRQKTQHGKQEGD- 120
            A  V      A  S+   R     QKRK EQ      +      G  ++ Q    +   
Sbjct: 281 EAARVDNLQERANSSKVTGRGSSSGQKRKAEQQPIPVSQRNFRPGGEFRRFQQKPFDAGE 340

Query: 121 -GNDKPKCNSCGRHHWGQCLEGKDVRFKCHKEGHMANNCPQKKVGGS 153
               KP C +CG+HH G+CL G    FKC +EGH A+ CP +  G +
Sbjct: 341 AARGKPLCATCGKHHLGRCLFGTRTCFKCRQEGHTADRCPMRLTGNA 387

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022934554.11.3e-0927.96uncharacterized protein LOC111441700 [Cucurbita moschata][more]
KAA0025929.16.5e-0928.93gag protease polyprotein [Cucumis melo var. makuwa][more]
KAA0047194.18.4e-0927.96DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa][more]
XP_022932138.18.4e-0933.53uncharacterized protein LOC111438460 [Cucurbita moschata][more]
KAA0033018.11.4e-0831.74gag protease polyprotein [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D4.10.60.10coord: 126..152
e-value: 2.5E-6
score: 29.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 59..114
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 93..114
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 68..82

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0008326.1Lag0008326.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003824 catalytic activity