Lag0005767 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0005767
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Methyltransferase
Location	chr6: 28982057 .. 28982770 (+)
RNA-Seq Expression	Lag0005767
Synteny	Lag0005767

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCGATCCATCATGGCCCATTCAAAATCCAATGTGGTCGGAATCACCATCAATGATTACCCAGTCAAACGTGCCAGATTGCACAACAAGAAGGCCGGACTGGACTCACTCTGCGAAGTCGTATGCAGAAATTTCCTCGAGATTTCGTTTTCTGAAGGGACCTTCGAGGGTGCTTACGCCATTGAAGCGACTTGCCAAGCGCCGAAACTCGAAGATGTTTACACGGAAGTGTTCAGAGTTTTGAAGTCGGGCTCGTTGTTCGTTTCGTTGGAGTGGGTTTCAACGGATAAATACGATGCCCAAAATCCAGAGCATGTGAAGATTATCGAAGAGATTGCGAGAGGGAACGCTTTACCTGGAGTGAAATCGTACATGGATGTGGCGAAGGCGGCGAAGAAAGTGGGATTTGAGATTCTGAAAGAGGAAGATCTGGCCAAGTCGTTGACGCTGCCGTGGTGGGGGCAATTAAAAATTGGCCGAGTTGTGTACTGGAGGAACCATATCCTCATGACAGTTCTCGCCACCTTGAGGATCGCGCCTAAAGGGACGTTGGAAGTTCACGAAATGTTGGTGGATGCCGCCGATTATTTGACCCGAAGCGCCGAGGTTGGAATCTTTTCGCCGATGTATTTGATCTTCTTTTTTGGCGCCGGAAAACAGAAGAGGGAGGGAGGGACCTCCTACCTCTGTTTTGACGCCGGAAAACGGAAGTGA

mRNA sequence

ATGCGATCCATCATGGCCCATTCAAAATCCAATGTGGTCGGAATCACCATCAATGATTACCCAGTCAAACGTGCCAGATTGCACAACAAGAAGGCCGGACTGGACTCACTCTGCGAAGTCGTATGCAGAAATTTCCTCGAGATTTCGTTTTCTGAAGGGACCTTCGAGGGTGCTTACGCCATTGAAGCGACTTGCCAAGCGCCGAAACTCGAAGATGTTTACACGGAAGTGTTCAGAGTTTTGAAGTCGGGCTCGTTGTTCGTTTCGTTGGAGTGGGTTTCAACGGATAAATACGATGCCCAAAATCCAGAGCATGTGAAGATTATCGAAGAGATTGCGAGAGGGAACGCTTTACCTGGAGTGAAATCGTACATGGATGTGGCGAAGGCGGCGAAGAAAGTGGGATTTGAGATTCTGAAAGAGGAAGATCTGGCCAAGTCGTTGACGCTGCCGTGGTGGGGGCAATTAAAAATTGGCCGAGTTGTGTACTGGAGGAACCATATCCTCATGACAGTTCTCGCCACCTTGAGGATCGCGCCTAAAGGGACGTTGGAAGTTCACGAAATGTTGGTGGATGCCGCCGATTATTTGACCCGAAGCGCCGAGGTTGGAATCTTTTCGCCGATGTATTTGATCTTCTTTTTTGGCGCCGGAAAACAGAAGAGGGAGGGAGGGACCTCCTACCTCTGTTTTGACGCCGGAAAACGGAAGTGA

Coding sequence (CDS)

ATGCGATCCATCATGGCCCATTCAAAATCCAATGTGGTCGGAATCACCATCAATGATTACCCAGTCAAACGTGCCAGATTGCACAACAAGAAGGCCGGACTGGACTCACTCTGCGAAGTCGTATGCAGAAATTTCCTCGAGATTTCGTTTTCTGAAGGGACCTTCGAGGGTGCTTACGCCATTGAAGCGACTTGCCAAGCGCCGAAACTCGAAGATGTTTACACGGAAGTGTTCAGAGTTTTGAAGTCGGGCTCGTTGTTCGTTTCGTTGGAGTGGGTTTCAACGGATAAATACGATGCCCAAAATCCAGAGCATGTGAAGATTATCGAAGAGATTGCGAGAGGGAACGCTTTACCTGGAGTGAAATCGTACATGGATGTGGCGAAGGCGGCGAAGAAAGTGGGATTTGAGATTCTGAAAGAGGAAGATCTGGCCAAGTCGTTGACGCTGCCGTGGTGGGGGCAATTAAAAATTGGCCGAGTTGTGTACTGGAGGAACCATATCCTCATGACAGTTCTCGCCACCTTGAGGATCGCGCCTAAAGGGACGTTGGAAGTTCACGAAATGTTGGTGGATGCCGCCGATTATTTGACCCGAAGCGCCGAGGTTGGAATCTTTTCGCCGATGTATTTGATCTTCTTTTTTGGCGCCGGAAAACAGAAGAGGGAGGGAGGGACCTCCTACCTCTGTTTTGACGCCGGAAAACGGAAGTGA

Protein sequence

MRSIMAHSKSNVVGITINDYPVKRARLHNKKAGLDSLCEVVCRNFLEISFSEGTFEGAYAIEATCQAPKLEDVYTEVFRVLKSGSLFVSLEWVSTDKYDAQNPEHVKIIEEIARGNALPGVKSYMDVAKAAKKVGFEILKEEDLAKSLTLPWWGQLKIGRVVYWRNHILMTVLATLRIAPKGTLEVHEMLVDAADYLTRSAEVGIFSPMYLIFFFGAGKQKREGGTSYLCFDAGKRK

Homology

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BLAST of Lag0005767 vs. NCBI nr
Match: XP_022937422.1 (24-methylenesterol C-methyltransferase 2 [Cucurbita moschata])

HSP 1 Score: 371.3 bits (952), Expect = 6.1e-99
Identity = 179/212 (84.43%), Postives = 194/212 (91.51%), Query Frame = 0


Query: 1   MRSIMAHSKSNVVGITINDYPVKRARLHNKKAGLDSLCEVVCRNFLEISFSEGTFEGAYA 60
           MRSI AHSK+NVVGITINDY V RARLHNKKAGLD+LCEVVC NFLE+ FSEGTF+GAYA
Sbjct: 138 MRSIAAHSKANVVGITINDYQVGRARLHNKKAGLDALCEVVCGNFLEMPFSEGTFDGAYA 197

Query: 61  IEATCQAPKLEDVYTEVFRVLKSGSLFVSLEWVSTDKYDAQNPEHVKIIEEIARGNALPG 120
           IEATC APKLEDVYTE++RVLK GSLFVSLEWVSTDKYD  NPEHVKIIEEIARGNALPG
Sbjct: 198 IEATCHAPKLEDVYTEIYRVLKPGSLFVSLEWVSTDKYDTANPEHVKIIEEIARGNALPG 257

Query: 121 VKSYMDVAKAAKKVGFEILKEEDLAKSLTLPWWGQLKIGRVVYWRNHILMTVLATLRIAP 180
           VKSY DVAKAAKKVGF++LKEEDLAK   LPWWG+LK+GR+ YWRNHIL+TVLA  RIAP
Sbjct: 258 VKSYDDVAKAAKKVGFQVLKEEDLAKPPALPWWGRLKMGRLAYWRNHILVTVLAAFRIAP 317

Query: 181 KGTLEVHEMLVDAADYLTRSAEVGIFSPMYLI 213
           KGTLEVHEMLV+AADYLTRS + GIFSPM+LI
Sbjct: 318 KGTLEVHEMLVEAADYLTRSGDAGIFSPMHLI 349

[Close]

BLAST of Lag0005767 vs. NCBI nr
Match: KAG6591249.1 (24-methylenesterol C-methyltransferase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 370.5 bits (950), Expect = 1.0e-98
Identity = 178/212 (83.96%), Postives = 194/212 (91.51%), Query Frame = 0


Query: 1   MRSIMAHSKSNVVGITINDYPVKRARLHNKKAGLDSLCEVVCRNFLEISFSEGTFEGAYA 60
           MRSI AHSK+NVVGITINDY V RARLHNKKAGLD+LCEVVC NFLE+ FSEGTF+GAYA
Sbjct: 138 MRSIAAHSKANVVGITINDYQVGRARLHNKKAGLDALCEVVCGNFLEMPFSEGTFDGAYA 197

Query: 61  IEATCQAPKLEDVYTEVFRVLKSGSLFVSLEWVSTDKYDAQNPEHVKIIEEIARGNALPG 120
           IEATC APKLEDVYTE++RVLK GSLFVSLEWVSTDKYD  NPEHVKIIEEIARGNALPG
Sbjct: 198 IEATCHAPKLEDVYTEIYRVLKPGSLFVSLEWVSTDKYDTANPEHVKIIEEIARGNALPG 257

Query: 121 VKSYMDVAKAAKKVGFEILKEEDLAKSLTLPWWGQLKIGRVVYWRNHILMTVLATLRIAP 180
           VKSY DVAKAAKKVGF++LKEEDLAK   LPWWG+LK+GR+ YWRNHIL+T+LA  RIAP
Sbjct: 258 VKSYHDVAKAAKKVGFQVLKEEDLAKPPALPWWGRLKMGRLAYWRNHILVTLLAAFRIAP 317

Query: 181 KGTLEVHEMLVDAADYLTRSAEVGIFSPMYLI 213
           KGTLEVHEMLV+AADYLTRS + GIFSPM+LI
Sbjct: 318 KGTLEVHEMLVEAADYLTRSGDAGIFSPMHLI 349

[Close]

BLAST of Lag0005767 vs. NCBI nr
Match: KAG7024132.1 (24-methylenesterol C-methyltransferase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 369.4 bits (947), Expect = 2.3e-98
Identity = 177/212 (83.49%), Postives = 194/212 (91.51%), Query Frame = 0


Query: 1   MRSIMAHSKSNVVGITINDYPVKRARLHNKKAGLDSLCEVVCRNFLEISFSEGTFEGAYA 60
           MRSI AHSK+NVVGITINDY V RARLHNKKAGLD+LCEVVC NFLE+ FSEGTF+GAYA
Sbjct: 138 MRSIAAHSKANVVGITINDYQVGRARLHNKKAGLDALCEVVCGNFLEMPFSEGTFDGAYA 197

Query: 61  IEATCQAPKLEDVYTEVFRVLKSGSLFVSLEWVSTDKYDAQNPEHVKIIEEIARGNALPG 120
           IEATC APKLEDVYTE++RVLK GSLFVSLEWVSTDKYD  NPEHVKII+EIARGNALPG
Sbjct: 198 IEATCHAPKLEDVYTEIYRVLKPGSLFVSLEWVSTDKYDTANPEHVKIIQEIARGNALPG 257

Query: 121 VKSYMDVAKAAKKVGFEILKEEDLAKSLTLPWWGQLKIGRVVYWRNHILMTVLATLRIAP 180
           VKSY DVAKAAKKVGF++LKEEDLAK   LPWWG+LK+GR+ YWRNHIL+T+LA  RIAP
Sbjct: 258 VKSYHDVAKAAKKVGFQVLKEEDLAKPPALPWWGRLKMGRLAYWRNHILVTLLAAFRIAP 317

Query: 181 KGTLEVHEMLVDAADYLTRSAEVGIFSPMYLI 213
           KGTLEVHEMLV+AADYLTRS + GIFSPM+LI
Sbjct: 318 KGTLEVHEMLVEAADYLTRSGDAGIFSPMHLI 349

[Close]

BLAST of Lag0005767 vs. NCBI nr
Match: XP_022976336.1 (24-methylenesterol C-methyltransferase 2 [Cucurbita maxima])

HSP 1 Score: 367.9 bits (943), Expect = 6.8e-98
Identity = 177/212 (83.49%), Postives = 193/212 (91.04%), Query Frame = 0


Query: 1   MRSIMAHSKSNVVGITINDYPVKRARLHNKKAGLDSLCEVVCRNFLEISFSEGTFEGAYA 60
           MRSI AHSK+NVVGITINDY V RARLHNKKAGLD+LCEVVC NFLE+ FSEGTF+GAYA
Sbjct: 138 MRSIAAHSKANVVGITINDYQVGRARLHNKKAGLDALCEVVCGNFLEMPFSEGTFDGAYA 197

Query: 61  IEATCQAPKLEDVYTEVFRVLKSGSLFVSLEWVSTDKYDAQNPEHVKIIEEIARGNALPG 120
           IEATC APKLEDVYTE++RVLK GSLFVSLEWVSTDKYD  NPEHV+IIEEIARGNALPG
Sbjct: 198 IEATCHAPKLEDVYTEIYRVLKPGSLFVSLEWVSTDKYDTANPEHVEIIEEIARGNALPG 257

Query: 121 VKSYMDVAKAAKKVGFEILKEEDLAKSLTLPWWGQLKIGRVVYWRNHILMTVLATLRIAP 180
           VKSY DVAKAAKKVGFE+LKEEDLAK   LPWW +LK+GR+ YWRNH+L+TVLA  RIAP
Sbjct: 258 VKSYDDVAKAAKKVGFEVLKEEDLAKPPALPWWDRLKMGRLAYWRNHVLVTVLAAFRIAP 317

Query: 181 KGTLEVHEMLVDAADYLTRSAEVGIFSPMYLI 213
           KGTLEVHEMLV+AADYLTRS + GIFSPM+LI
Sbjct: 318 KGTLEVHEMLVEAADYLTRSGDAGIFSPMHLI 349

[Close]

BLAST of Lag0005767 vs. NCBI nr
Match: XP_038899067.1 (24-methylenesterol C-methyltransferase 2 [Benincasa hispida])

HSP 1 Score: 367.9 bits (943), Expect = 6.8e-98
Identity = 177/212 (83.49%), Postives = 194/212 (91.51%), Query Frame = 0


Query: 1   MRSIMAHSKSNVVGITINDYPVKRARLHNKKAGLDSLCEVVCRNFLEISFSEGTFEGAYA 60
           MRSI AHSK+NVVGITINDY V+RARLHNKKAGLDSLCEVVC NFLE+ FS+ +F GAYA
Sbjct: 138 MRSIAAHSKANVVGITINDYQVQRARLHNKKAGLDSLCEVVCGNFLEMPFSDESFNGAYA 197

Query: 61  IEATCQAPKLEDVYTEVFRVLKSGSLFVSLEWVSTDKYDAQNPEHVKIIEEIARGNALPG 120
           IEATC APKLEDVY+E++RVLK GSLFVSLEWVSTDKYD  NPEHVKIIEEIARGNALPG
Sbjct: 198 IEATCHAPKLEDVYSEIYRVLKPGSLFVSLEWVSTDKYDTTNPEHVKIIEEIARGNALPG 257

Query: 121 VKSYMDVAKAAKKVGFEILKEEDLAKSLTLPWWGQLKIGRVVYWRNHILMTVLATLRIAP 180
           VKSY DVAK+AKKVGFE+LKEEDLAK   LPWWG+LK+GRV YWRNHIL+T+LA LRIAP
Sbjct: 258 VKSYDDVAKSAKKVGFEVLKEEDLAKPPALPWWGRLKMGRVAYWRNHILVTILAALRIAP 317

Query: 181 KGTLEVHEMLVDAADYLTRSAEVGIFSPMYLI 213
           KGTLEVHEMLV+AADYLTRS + GIFSPM+LI
Sbjct: 318 KGTLEVHEMLVEAADYLTRSGDAGIFSPMHLI 349

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BLAST of Lag0005767 vs. ExPASy Swiss-Prot
Match: Q39227 (24-methylenesterol C-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=SMT2 PE=1 SV=2)

HSP 1 Score: 302.0 bits (772), Expect = 6.0e-81
Identity = 133/212 (62.74%), Postives = 180/212 (84.91%), Query Frame = 0


Query: 1   MRSIMAHSKSNVVGITINDYPVKRARLHNKKAGLDSLCEVVCRNFLEISFSEGTFEGAYA 60
           MR+I +HS++NVVGITIN+Y V RARLHNKKAGLD+LCEVVC NFL++ F + +F+GAY+
Sbjct: 138 MRAIASHSRANVVGITINEYQVNRARLHNKKAGLDALCEVVCGNFLQMPFDDNSFDGAYS 197

Query: 61  IEATCQAPKLEDVYTEVFRVLKSGSLFVSLEWVSTDKYDAQNPEHVKIIEEIARGNALPG 120
           IEATC APKLE+VY E++RVLK GS++VS EWV+T+K+ A++ EHV++I+ I RG+ALPG
Sbjct: 198 IEATCHAPKLEEVYAEIYRVLKPGSMYVSYEWVTTEKFKAEDDEHVEVIQGIERGDALPG 257

Query: 121 VKSYMDVAKAAKKVGFEILKEEDLAKSLTLPWWGQLKIGRVVYWRNHILMTVLATLRIAP 180
           +++Y+D+A+ AKKVGFEI+KE+DLA     PWW +LK+GR+ YWRNHI++ +L+ + +AP
Sbjct: 258 LRAYVDIAETAKKVGFEIVKEKDLASPPAEPWWTRLKMGRLAYWRNHIVVQILSAVGVAP 317

Query: 181 KGTLEVHEMLVDAADYLTRSAEVGIFSPMYLI 213
           KGT++VHEML   ADYLTR  E GIFSPM++I
Sbjct: 318 KGTVDVHEMLFKTADYLTRGGETGIFSPMHMI 349

[Close]

BLAST of Lag0005767 vs. ExPASy Swiss-Prot
Match: Q94JS4 (24-methylenesterol C-methyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=SMT3 PE=2 SV=1)

HSP 1 Score: 300.8 bits (769), Expect = 1.3e-80
Identity = 134/212 (63.21%), Postives = 175/212 (82.55%), Query Frame = 0


Query: 1   MRSIMAHSKSNVVGITINDYPVKRARLHNKKAGLDSLCEVVCRNFLEISFSEGTFEGAYA 60
           MR+I AHSK+ V GITIN+Y V+RA+LHNKKAGLDSLC VVC NFL++ F E TF+GAY+
Sbjct: 138 MRAIAAHSKAQVTGITINEYQVQRAKLHNKKAGLDSLCNVVCGNFLKMPFDENTFDGAYS 197

Query: 61  IEATCQAPKLEDVYTEVFRVLKSGSLFVSLEWVSTDKYDAQNPEHVKIIEEIARGNALPG 120
           IEATC APKLE+VY+E+FRV+K GSLFVS EWV+T+KY   + EH  +I+ I RG+ALPG
Sbjct: 198 IEATCHAPKLEEVYSEIFRVMKPGSLFVSYEWVTTEKYRDDDEEHKDVIQGIERGDALPG 257

Query: 121 VKSYMDVAKAAKKVGFEILKEEDLAKSLTLPWWGQLKIGRVVYWRNHILMTVLATLRIAP 180
           ++SY D+A  AKKVGFE++KE+DLAK  + PWW +LK+GR+ YWRNH+++ +L+ + +AP
Sbjct: 258 LRSYADIAVTAKKVGFEVVKEKDLAKPPSKPWWNRLKMGRIAYWRNHVVVVILSAIGVAP 317

Query: 181 KGTLEVHEMLVDAADYLTRSAEVGIFSPMYLI 213
           KGT++VH+ML   ADYLTR  E GIFSPM++I
Sbjct: 318 KGTVDVHKMLFKTADYLTRGGETGIFSPMHMI 349

[Close]

BLAST of Lag0005767 vs. ExPASy Swiss-Prot
Match: O82427 (24-methylenesterol C-methyltransferase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Smt2-1 PE=2 SV=2)

HSP 1 Score: 284.3 bits (726), Expect = 1.3e-75
Identity = 133/212 (62.74%), Postives = 165/212 (77.83%), Query Frame = 0


Query: 1   MRSIMAHSKSNVVGITINDYPVKRARLHNKKAGLDSLCEVVCRNFLEISFSEGTFEGAYA 60
           MR+I AHS SNVVGITIN+Y V RAR HN+KAGLDS CEVVC NFL + FS+ +F+GAY+
Sbjct: 143 MRAIAAHSGSNVVGITINEYQVNRARAHNRKAGLDSRCEVVCGNFLSMPFSDASFDGAYS 202

Query: 61  IEATCQAPKLEDVYTEVFRVLKSGSLFVSLEWVSTDKYDAQNPEHVKIIEEIARGNALPG 120
           IEATC AP+L+DVY EVFRVLK G L+VS EWV+T  Y A NPEHV+ I  I RG+ALPG
Sbjct: 203 IEATCHAPRLQDVYGEVFRVLKPGGLYVSYEWVTTSLYRADNPEHVEAIHGIERGDALPG 262

Query: 121 VKSYMDVAKAAKKVGFEILKEEDLAKSLTLPWWGQLKIGRVVYWRNHILMTVLATLRIAP 180
           ++   ++A  AK+VGFE+LKE DLA    LPWW +LK+GR+ YWRN +++ VL  LRIAP
Sbjct: 263 LRRQDEIASIAKEVGFEVLKELDLALPPALPWWTRLKMGRIAYWRNSLVVRVLTMLRIAP 322

Query: 181 KGTLEVHEMLVDAADYLTRSAEVGIFSPMYLI 213
           KG  EVHEML + A +LTR  E GIF+PM+++
Sbjct: 323 KGVCEVHEMLYETAQHLTRGGETGIFTPMHMV 354

[Close]

BLAST of Lag0005767 vs. ExPASy Swiss-Prot
Match: H2E7T9 (Sterol methyltransferase-like 2 OS=Botryococcus braunii OX=38881 GN=SMT-2 PE=2 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 2.5e-42
Identity = 95/217 (43.78%), Postives = 133/217 (61.29%), Query Frame = 0


Query: 2   RSIMAHSKSNVVGITINDYPVKRARLHNKKAGLDSLCEVVCRNFLEISFSEGTFEGAYAI 61
           R++ A + ++V GITIN+Y VKRA  H KKAGL  L   V  +F ++ F++ TF+ A+AI
Sbjct: 168 RTVAALTGAHVTGITINEYQVKRALYHTKKAGLTGLFTPVQGDFTDMPFADKTFDAAFAI 227

Query: 62  EATCQAPKLEDVYTEVFRVLKSGSLFVSLEWVSTDKYDAQNPEHVKIIEEIARGNALPGV 121
           EATC APKLE VY E+FRVLK G+ F   E V+TDK+D  N  HV+II  +  GN +P +
Sbjct: 228 EATCHAPKLEQVYGEIFRVLKPGAFFAVYEAVTTDKFDPANKRHVEIINSLVYGNGIPDM 287

Query: 122 KSYMDVAKAAKKVGFEILKEED--LAKSLTLPWWGQLK----IGRVVYWRNHILMTVLAT 181
           +++    +A K VGF++    D   A  + LPWW ++K     G V Y +   L   L +
Sbjct: 288 RTWKQAEEAGKNVGFKLCCAFDAGAASPVALPWWERVKDMINWGVVKYTKAACL--ALDS 347

Query: 182 LRIAPKGTLEVHEMLVDAADYLTRSAEVGIFSPMYLI 213
           LR+ PK   +V  M+ D+   L  S E GIF+PMYL+
Sbjct: 348 LRLLPKDYWKVANMVGDSLPDLVESGETGIFTPMYLL 382

[Close]

BLAST of Lag0005767 vs. ExPASy Swiss-Prot
Match: H2E7T7 (Botryococcene C-methyltransferase OS=Botryococcus braunii OX=38881 GN=TMT-3 PE=1 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 1.2e-41
Identity = 91/219 (41.55%), Postives = 134/219 (61.19%), Query Frame = 0


Query: 2   RSIMAHSKSNVVGITINDYPVKRARLHNKKAGLDSLCEVVCRNFLEISFSEGTFEGAYAI 61
           R++ A S + V GITIN Y +KRA LH K+AGL    + V  NF  + F + +F+ A+A+
Sbjct: 159 RTVAACSGAVVTGITINAYQIKRAELHTKRAGLVGYFKPVQGNFCAMPFQDKSFDAAFAM 218

Query: 62  EATCQAPKLEDVYTEVFRVLKSGSLFVSLEWVSTDKYDAQNPEHVKIIEEIARGNALPGV 121
           ++TC APKLEDVY+EVFRVLK G+ F + EWVST  YD+ NPEHVK +  I  GN LP +
Sbjct: 219 DSTCHAPKLEDVYSEVFRVLKPGAYFATYEWVSTKNYDSNNPEHVKCMNSIILGNGLPNI 278

Query: 122 KSYMDVAKAAKKVGFEILKEEDLAKSLTL--PWWGQLKIGRVVYW---RNH-ILMTVLAT 181
           +S+    +A K VGF +L   D+A +  +  PW+   +  R+V W   R H   +   +T
Sbjct: 279 RSWKQAEEAGKNVGFNLLTSLDMATNSPIGKPWYSVPE--RMVNWGLFRFHKACIRTAST 338

Query: 182 LRIAPKGTLEVHEMLVDAADYLTRSAEVGIFSPMYLIFF 215
           L + P  + +   +L + A+ L +  +  IF+PM+L+ F
Sbjct: 339 LHLLPPESWKFFYILAEMAENLVKGGQWDIFTPMHLLIF 375

[Close]

BLAST of Lag0005767 vs. ExPASy TrEMBL
Match: A0A6J1FB58 (Methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111443718 PE=3 SV=1)

HSP 1 Score: 371.3 bits (952), Expect = 3.0e-99
Identity = 179/212 (84.43%), Postives = 194/212 (91.51%), Query Frame = 0


Query: 1   MRSIMAHSKSNVVGITINDYPVKRARLHNKKAGLDSLCEVVCRNFLEISFSEGTFEGAYA 60
           MRSI AHSK+NVVGITINDY V RARLHNKKAGLD+LCEVVC NFLE+ FSEGTF+GAYA
Sbjct: 138 MRSIAAHSKANVVGITINDYQVGRARLHNKKAGLDALCEVVCGNFLEMPFSEGTFDGAYA 197

Query: 61  IEATCQAPKLEDVYTEVFRVLKSGSLFVSLEWVSTDKYDAQNPEHVKIIEEIARGNALPG 120
           IEATC APKLEDVYTE++RVLK GSLFVSLEWVSTDKYD  NPEHVKIIEEIARGNALPG
Sbjct: 198 IEATCHAPKLEDVYTEIYRVLKPGSLFVSLEWVSTDKYDTANPEHVKIIEEIARGNALPG 257

Query: 121 VKSYMDVAKAAKKVGFEILKEEDLAKSLTLPWWGQLKIGRVVYWRNHILMTVLATLRIAP 180
           VKSY DVAKAAKKVGF++LKEEDLAK   LPWWG+LK+GR+ YWRNHIL+TVLA  RIAP
Sbjct: 258 VKSYDDVAKAAKKVGFQVLKEEDLAKPPALPWWGRLKMGRLAYWRNHILVTVLAAFRIAP 317

Query: 181 KGTLEVHEMLVDAADYLTRSAEVGIFSPMYLI 213
           KGTLEVHEMLV+AADYLTRS + GIFSPM+LI
Sbjct: 318 KGTLEVHEMLVEAADYLTRSGDAGIFSPMHLI 349

[Close]

BLAST of Lag0005767 vs. ExPASy TrEMBL
Match: A0A6J1IGM5 (Methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111476770 PE=3 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 3.3e-98
Identity = 177/212 (83.49%), Postives = 193/212 (91.04%), Query Frame = 0


Query: 1   MRSIMAHSKSNVVGITINDYPVKRARLHNKKAGLDSLCEVVCRNFLEISFSEGTFEGAYA 60
           MRSI AHSK+NVVGITINDY V RARLHNKKAGLD+LCEVVC NFLE+ FSEGTF+GAYA
Sbjct: 138 MRSIAAHSKANVVGITINDYQVGRARLHNKKAGLDALCEVVCGNFLEMPFSEGTFDGAYA 197

Query: 61  IEATCQAPKLEDVYTEVFRVLKSGSLFVSLEWVSTDKYDAQNPEHVKIIEEIARGNALPG 120
           IEATC APKLEDVYTE++RVLK GSLFVSLEWVSTDKYD  NPEHV+IIEEIARGNALPG
Sbjct: 198 IEATCHAPKLEDVYTEIYRVLKPGSLFVSLEWVSTDKYDTANPEHVEIIEEIARGNALPG 257

Query: 121 VKSYMDVAKAAKKVGFEILKEEDLAKSLTLPWWGQLKIGRVVYWRNHILMTVLATLRIAP 180
           VKSY DVAKAAKKVGFE+LKEEDLAK   LPWW +LK+GR+ YWRNH+L+TVLA  RIAP
Sbjct: 258 VKSYDDVAKAAKKVGFEVLKEEDLAKPPALPWWDRLKMGRLAYWRNHVLVTVLAAFRIAP 317

Query: 181 KGTLEVHEMLVDAADYLTRSAEVGIFSPMYLI 213
           KGTLEVHEMLV+AADYLTRS + GIFSPM+LI
Sbjct: 318 KGTLEVHEMLVEAADYLTRSGDAGIFSPMHLI 349

[Close]

BLAST of Lag0005767 vs. ExPASy TrEMBL
Match: A0A5A7VA53 (Methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G007740 PE=3 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 5.6e-98
Identity = 176/212 (83.02%), Postives = 194/212 (91.51%), Query Frame = 0


Query: 1   MRSIMAHSKSNVVGITINDYPVKRARLHNKKAGLDSLCEVVCRNFLEISFSEGTFEGAYA 60
           MRSI AHSK+NVVGITINDY V+RARLHN+KAGLDSLCEVVC NFLE+ FS+ TF GAYA
Sbjct: 138 MRSIAAHSKANVVGITINDYQVQRARLHNRKAGLDSLCEVVCGNFLEMPFSDETFNGAYA 197

Query: 61  IEATCQAPKLEDVYTEVFRVLKSGSLFVSLEWVSTDKYDAQNPEHVKIIEEIARGNALPG 120
           IEATC APKLEDVY+E++RVLK GSLFVSLEWVSTDKYD  NPEHVKIIEEIARGNALPG
Sbjct: 198 IEATCHAPKLEDVYSEIYRVLKPGSLFVSLEWVSTDKYDTSNPEHVKIIEEIARGNALPG 257

Query: 121 VKSYMDVAKAAKKVGFEILKEEDLAKSLTLPWWGQLKIGRVVYWRNHILMTVLATLRIAP 180
           VKSY DVAK+AKKVGFE+LKEEDLAK   LPWW +LK+GRV YWRNHIL+T+LATLRIAP
Sbjct: 258 VKSYSDVAKSAKKVGFEVLKEEDLAKPPALPWWERLKMGRVAYWRNHILVTILATLRIAP 317

Query: 181 KGTLEVHEMLVDAADYLTRSAEVGIFSPMYLI 213
           KGTL+VHEMLV+AADYLTRS + GIFSPM+LI
Sbjct: 318 KGTLDVHEMLVEAADYLTRSGDAGIFSPMHLI 349

[Close]

BLAST of Lag0005767 vs. ExPASy TrEMBL
Match: A0A1S3CRG4 (Methyltransferase OS=Cucumis melo OX=3656 GN=LOC103503852 PE=3 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 5.6e-98
Identity = 176/212 (83.02%), Postives = 194/212 (91.51%), Query Frame = 0


Query: 1   MRSIMAHSKSNVVGITINDYPVKRARLHNKKAGLDSLCEVVCRNFLEISFSEGTFEGAYA 60
           MRSI AHSK+NVVGITINDY V+RARLHN+KAGLDSLCEVVC NFLE+ FS+ TF GAYA
Sbjct: 138 MRSIAAHSKANVVGITINDYQVQRARLHNRKAGLDSLCEVVCGNFLEMPFSDETFNGAYA 197

Query: 61  IEATCQAPKLEDVYTEVFRVLKSGSLFVSLEWVSTDKYDAQNPEHVKIIEEIARGNALPG 120
           IEATC APKLEDVY+E++RVLK GSLFVSLEWVSTDKYD  NPEHVKIIEEIARGNALPG
Sbjct: 198 IEATCHAPKLEDVYSEIYRVLKPGSLFVSLEWVSTDKYDTSNPEHVKIIEEIARGNALPG 257

Query: 121 VKSYMDVAKAAKKVGFEILKEEDLAKSLTLPWWGQLKIGRVVYWRNHILMTVLATLRIAP 180
           VKSY DVAK+AKKVGFE+LKEEDLAK   LPWW +LK+GRV YWRNHIL+T+LATLRIAP
Sbjct: 258 VKSYSDVAKSAKKVGFEVLKEEDLAKPPALPWWERLKMGRVAYWRNHILVTILATLRIAP 317

Query: 181 KGTLEVHEMLVDAADYLTRSAEVGIFSPMYLI 213
           KGTL+VHEMLV+AADYLTRS + GIFSPM+LI
Sbjct: 318 KGTLDVHEMLVEAADYLTRSGDAGIFSPMHLI 349

[Close]

BLAST of Lag0005767 vs. ExPASy TrEMBL
Match: A0A0A0LJ82 (Methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_3G875420 PE=3 SV=1)

HSP 1 Score: 362.1 bits (928), Expect = 1.8e-96
Identity = 174/212 (82.08%), Postives = 193/212 (91.04%), Query Frame = 0


Query: 1   MRSIMAHSKSNVVGITINDYPVKRARLHNKKAGLDSLCEVVCRNFLEISFSEGTFEGAYA 60
           MRSI A+SK+NVVGITINDY V+RARLHN+KAGLDSLCEVVC NFLE+ FS+ TF GAYA
Sbjct: 138 MRSIAAYSKANVVGITINDYQVQRARLHNRKAGLDSLCEVVCGNFLEMPFSDETFNGAYA 197

Query: 61  IEATCQAPKLEDVYTEVFRVLKSGSLFVSLEWVSTDKYDAQNPEHVKIIEEIARGNALPG 120
           IEATC APKLEDVY+E++RVLK GSLFVSLEWVSTDKYD  NPEHVKIIEEIARGNALPG
Sbjct: 198 IEATCHAPKLEDVYSEIYRVLKPGSLFVSLEWVSTDKYDTANPEHVKIIEEIARGNALPG 257

Query: 121 VKSYMDVAKAAKKVGFEILKEEDLAKSLTLPWWGQLKIGRVVYWRNHILMTVLATLRIAP 180
           VK Y DVAK+AKKVGFE+LKEEDLAK   LPWW +LK+GRV YWRNHIL+T+LATLRIAP
Sbjct: 258 VKLYSDVAKSAKKVGFEVLKEEDLAKPPALPWWERLKMGRVAYWRNHILVTILATLRIAP 317

Query: 181 KGTLEVHEMLVDAADYLTRSAEVGIFSPMYLI 213
           KGTL+VHEMLV+AADYLTRS + GIFSPM+LI
Sbjct: 318 KGTLDVHEMLVEAADYLTRSGDAGIFSPMHLI 349

[Close]

BLAST of Lag0005767 vs. TAIR 10
Match: AT1G20330.1 (sterol methyltransferase 2 )

HSP 1 Score: 302.0 bits (772), Expect = 4.3e-82
Identity = 133/212 (62.74%), Postives = 180/212 (84.91%), Query Frame = 0


Query: 1   MRSIMAHSKSNVVGITINDYPVKRARLHNKKAGLDSLCEVVCRNFLEISFSEGTFEGAYA 60
           MR+I +HS++NVVGITIN+Y V RARLHNKKAGLD+LCEVVC NFL++ F + +F+GAY+
Sbjct: 138 MRAIASHSRANVVGITINEYQVNRARLHNKKAGLDALCEVVCGNFLQMPFDDNSFDGAYS 197

Query: 61  IEATCQAPKLEDVYTEVFRVLKSGSLFVSLEWVSTDKYDAQNPEHVKIIEEIARGNALPG 120
           IEATC APKLE+VY E++RVLK GS++VS EWV+T+K+ A++ EHV++I+ I RG+ALPG
Sbjct: 198 IEATCHAPKLEEVYAEIYRVLKPGSMYVSYEWVTTEKFKAEDDEHVEVIQGIERGDALPG 257

Query: 121 VKSYMDVAKAAKKVGFEILKEEDLAKSLTLPWWGQLKIGRVVYWRNHILMTVLATLRIAP 180
           +++Y+D+A+ AKKVGFEI+KE+DLA     PWW +LK+GR+ YWRNHI++ +L+ + +AP
Sbjct: 258 LRAYVDIAETAKKVGFEIVKEKDLASPPAEPWWTRLKMGRLAYWRNHIVVQILSAVGVAP 317

Query: 181 KGTLEVHEMLVDAADYLTRSAEVGIFSPMYLI 213
           KGT++VHEML   ADYLTR  E GIFSPM++I
Sbjct: 318 KGTVDVHEMLFKTADYLTRGGETGIFSPMHMI 349

[Close]

BLAST of Lag0005767 vs. TAIR 10
Match: AT1G76090.1 (sterol methyltransferase 3 )

HSP 1 Score: 300.8 bits (769), Expect = 9.5e-82
Identity = 134/212 (63.21%), Postives = 175/212 (82.55%), Query Frame = 0


Query: 1   MRSIMAHSKSNVVGITINDYPVKRARLHNKKAGLDSLCEVVCRNFLEISFSEGTFEGAYA 60
           MR+I AHSK+ V GITIN+Y V+RA+LHNKKAGLDSLC VVC NFL++ F E TF+GAY+
Sbjct: 138 MRAIAAHSKAQVTGITINEYQVQRAKLHNKKAGLDSLCNVVCGNFLKMPFDENTFDGAYS 197

Query: 61  IEATCQAPKLEDVYTEVFRVLKSGSLFVSLEWVSTDKYDAQNPEHVKIIEEIARGNALPG 120
           IEATC APKLE+VY+E+FRV+K GSLFVS EWV+T+KY   + EH  +I+ I RG+ALPG
Sbjct: 198 IEATCHAPKLEEVYSEIFRVMKPGSLFVSYEWVTTEKYRDDDEEHKDVIQGIERGDALPG 257

Query: 121 VKSYMDVAKAAKKVGFEILKEEDLAKSLTLPWWGQLKIGRVVYWRNHILMTVLATLRIAP 180
           ++SY D+A  AKKVGFE++KE+DLAK  + PWW +LK+GR+ YWRNH+++ +L+ + +AP
Sbjct: 258 LRSYADIAVTAKKVGFEVVKEKDLAKPPSKPWWNRLKMGRIAYWRNHVVVVILSAIGVAP 317

Query: 181 KGTLEVHEMLVDAADYLTRSAEVGIFSPMYLI 213
           KGT++VH+ML   ADYLTR  E GIFSPM++I
Sbjct: 318 KGTVDVHKMLFKTADYLTRGGETGIFSPMHMI 349

[Close]

BLAST of Lag0005767 vs. TAIR 10
Match: AT5G13710.1 (sterol methyltransferase 1 )

HSP 1 Score: 151.4 bits (381), Expect = 9.3e-37
Identity = 83/229 (36.24%), Postives = 122/229 (53.28%), Query Frame = 0


Query: 1   MRSIMAHSKSNVVGITINDYPVKRARLHNKKAGLDSLCEVVCRNFLEISFSEGTFEGAYA 60
           +R I   S S V G+  N+Y + R +  N+ AG+D  C  V  +F+++ F E +F+  YA
Sbjct: 109 LREIARFSNSVVTGLNNNEYQITRGKELNRLAGVDKTCNFVKADFMKMPFPENSFDAVYA 168

Query: 61  IEATCQAPKLEDVYTEVFRVLKSGSLFVSLEWVSTDKYDAQNPEHVKIIEEIARGNALPG 120
           IEATC AP     Y E++RVLK G  F + EW  TD +D  N EH KI  EI  G+ LP 
Sbjct: 169 IEATCHAPDAYGCYKEIYRVLKPGQCFAAYEWCMTDAFDPDNAEHQKIKGEIEIGDGLPD 228

Query: 121 VKSYMDVAKAAKKVGFEILKEEDLAKSLTLPWW----------GQLKIGRVVYWRNHILM 180
           ++      +A K+ GFE++ E+DLAK   +PW+             ++  V  +    ++
Sbjct: 229 IRLTTKCLEALKQAGFEVIWEKDLAKDSPVPWYLPLDKNHFSLSSFRLTAVGRFITKNMV 288

Query: 181 TVLATLRIAPKGTLEVHEMLVDAADYLTRSAEVGIFSPMYLIFFFGAGK 220
            +L  +R+AP+G+  V   L  AA+ L       IF+PMY   FF A K
Sbjct: 289 KILEYIRLAPQGSQRVSNFLEQAAEGLVDGGRREIFTPMY---FFLARK 334

[Close]

BLAST of Lag0005767 vs. TAIR 10
Match: AT5G13710.2 (sterol methyltransferase 1 )

HSP 1 Score: 151.4 bits (381), Expect = 9.3e-37
Identity = 83/229 (36.24%), Postives = 122/229 (53.28%), Query Frame = 0


Query: 1   MRSIMAHSKSNVVGITINDYPVKRARLHNKKAGLDSLCEVVCRNFLEISFSEGTFEGAYA 60
           +R I   S S V G+  N+Y + R +  N+ AG+D  C  V  +F+++ F E +F+  YA
Sbjct: 109 LREIARFSNSVVTGLNNNEYQITRGKELNRLAGVDKTCNFVKADFMKMPFPENSFDAVYA 168

Query: 61  IEATCQAPKLEDVYTEVFRVLKSGSLFVSLEWVSTDKYDAQNPEHVKIIEEIARGNALPG 120
           IEATC AP     Y E++RVLK G  F + EW  TD +D  N EH KI  EI  G+ LP 
Sbjct: 169 IEATCHAPDAYGCYKEIYRVLKPGQCFAAYEWCMTDAFDPDNAEHQKIKGEIEIGDGLPD 228

Query: 121 VKSYMDVAKAAKKVGFEILKEEDLAKSLTLPWW----------GQLKIGRVVYWRNHILM 180
           ++      +A K+ GFE++ E+DLAK   +PW+             ++  V  +    ++
Sbjct: 229 IRLTTKCLEALKQAGFEVIWEKDLAKDSPVPWYLPLDKNHFSLSSFRLTAVGRFITKNMV 288

Query: 181 TVLATLRIAPKGTLEVHEMLVDAADYLTRSAEVGIFSPMYLIFFFGAGK 220
            +L  +R+AP+G+  V   L  AA+ L       IF+PMY   FF A K
Sbjct: 289 KILEYIRLAPQGSQRVSNFLEQAAEGLVDGGRREIFTPMY---FFLARK 334

The following BLAST results are available for this feature:

BLAST of Lag0005767 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022937422.1	6.1e-99	84.43	24-methylenesterol C-methyltransferase 2 [Cucurbita moschata]	[more]
KAG6591249.1	1.0e-98	83.96	24-methylenesterol C-methyltransferase 2, partial [Cucurbita argyrosperma subsp....	[more]
KAG7024132.1	2.3e-98	83.49	24-methylenesterol C-methyltransferase 2, partial [Cucurbita argyrosperma subsp....	[more]
XP_022976336.1	6.8e-98	83.49	24-methylenesterol C-methyltransferase 2 [Cucurbita maxima]	[more]
XP_038899067.1	6.8e-98	83.49	24-methylenesterol C-methyltransferase 2 [Benincasa hispida]	[more]

BLAST of Lag0005767 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q39227	6.0e-81	62.74	24-methylenesterol C-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=SMT2...	[more]
Q94JS4	1.3e-80	63.21	24-methylenesterol C-methyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=SMT3...	[more]
O82427	1.3e-75	62.74	24-methylenesterol C-methyltransferase 2 OS=Oryza sativa subsp. japonica OX=3994...	[more]
H2E7T9	2.5e-42	43.78	Sterol methyltransferase-like 2 OS=Botryococcus braunii OX=38881 GN=SMT-2 PE=2 S...	[more]
H2E7T7	1.2e-41	41.55	Botryococcene C-methyltransferase OS=Botryococcus braunii OX=38881 GN=TMT-3 PE=1...	[more]

BLAST of Lag0005767 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FB58	3.0e-99	84.43	Methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111443718 PE=3 SV=1	[more]
A0A6J1IGM5	3.3e-98	83.49	Methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111476770 PE=3 SV=1	[more]
A0A5A7VA53	5.6e-98	83.02	Methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G00...	[more]
A0A1S3CRG4	5.6e-98	83.02	Methyltransferase OS=Cucumis melo OX=3656 GN=LOC103503852 PE=3 SV=1	[more]
A0A0A0LJ82	1.8e-96	82.08	Methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_3G875420 PE=3 SV=1	[more]

BLAST of Lag0005767 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G20330.1	4.3e-82	62.74	sterol methyltransferase 2	[more]
AT1G76090.1	9.5e-82	63.21	sterol methyltransferase 3	[more]
AT5G13710.1	9.3e-37	36.24	sterol methyltransferase 1	[more]
AT5G13710.2	9.3e-37	36.24	sterol methyltransferase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 1..164 e-value: 5.8E-24 score: 86.7
None	No IPR available	PANTHER	PTHR44742:SF7	METHYLTRANSFERASE	coord: 1..213
IPR013216	Methyltransferase type 11	PFAM	PF08241	Methyltransf_11	coord: 6..88 e-value: 6.2E-8 score: 33.2
IPR013705	Sterol methyltransferase C-terminal	PFAM	PF08498	Sterol_MT_C	coord: 156..212 e-value: 5.1E-16 score: 58.6
IPR044625	Sterol methyltransferase SMT2/3-like	PANTHER	PTHR44742	FAMILY NOT NAMED	coord: 1..213
IPR030384	SAM-dependent methyltransferase SMT-type	PROSITE	PS51685	SAM_MT_ERG6_SMT	coord: 1..217 score: 58.912685
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 9..206

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0005767.1	Lag0005767.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
biological_process	GO:0090378	seed trichome elongation
biological_process	GO:0016126	sterol biosynthetic process
biological_process	GO:0006694	steroid biosynthetic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0008168	methyltransferase activity