Lag0002655 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0002655 vs. NCBI nr
Match: XP_038898256.1 (beta-carotene isomerase D27, chloroplastic [Benincasa hispida])

HSP 1 Score: 388.7 bits (997), Expect = 4.3e-104
Identity = 198/251 (78.88%), Postives = 209/251 (83.27%), Query Frame = 0

Query: 1   MKPLALLHPPAISSAPLHFS-SKSHLRNRHFNFSCSALQSQSVRFQSSPRRLAIQLQLNS 60
           MKPLALL PP ISSAPL F+ SKSHLRN   N SCSALQS+S                  
Sbjct: 1   MKPLALLRPPTISSAPLRFTFSKSHLRNHPLNLSCSALQSES------------------ 60

Query: 61  VSPFSSSCLHPYIKGETSSKPEYKPGILDDFFLNLFRSKMVQEVGWDSEKPGYDGLIEVA 120
                       IK ETSSKPEYKPG+LD+FFLN+FRSKMVQEVGWDSEKPGYDGLIEVA
Sbjct: 61  ------------IKNETSSKPEYKPGLLDEFFLNVFRSKMVQEVGWDSEKPGYDGLIEVA 120

Query: 121 NRLTMKGKTNSDTIEASVRILTALFPPLLLELYRMLVSPIAGGKVAAIMVARVTALTCQW 180
           NRLT KGKTNSDTIEASVRILTALFPPLLL+LYR+LVSPIAGGKVAAIMVARVTALTCQW
Sbjct: 121 NRLTTKGKTNSDTIEASVRILTALFPPLLLDLYRILVSPIAGGKVAAIMVARVTALTCQW 180

Query: 181 LMGTCTVNSIDLPDGSSCQSGVFVEKCKYLEESKCIGICINTCKFPTQSFFKEQMGVPLL 240
           LMGTCTVNS +LPDGSSCQSGVFVEKCKYLEESKCIGICINTCK PTQSFFK++MG+PLL
Sbjct: 181 LMGTCTVNSFELPDGSSCQSGVFVEKCKYLEESKCIGICINTCKLPTQSFFKDRMGIPLL 221

Query: 241 MEPNFNDYSCQ 251
           MEPNFNDYSCQ
Sbjct: 241 MEPNFNDYSCQ 221

BLAST of Lag0002655 vs. NCBI nr
Match: XP_023004624.1 (beta-carotene isomerase D27, chloroplastic [Cucurbita maxima])

HSP 1 Score: 385.6 bits (989), Expect = 3.6e-103
Identity = 200/252 (79.37%), Postives = 209/252 (82.94%), Query Frame = 0

Query: 1   MKPLA-LLHPPA-ISSAPLHFSSKSHLRNRHFNFSCSALQSQSVRFQSSPRRLAIQLQLN 60
           MKP+A LLHPPA ISSA L FSSKSHLR R  NFSC A+QS+S                 
Sbjct: 1   MKPVALLLHPPATISSAALRFSSKSHLRKRPLNFSCFAVQSEST---------------- 60

Query: 61  SVSPFSSSCLHPYIKGETSSKPEYKPGILDDFFLNLFRSKMVQEVGWDSEKPGYDGLIEV 120
                         K E SSKP+YKPG+LDDFFLNLFRSKMVQEVGWDSEKPGYDGLIEV
Sbjct: 61  --------------KVEVSSKPDYKPGMLDDFFLNLFRSKMVQEVGWDSEKPGYDGLIEV 120

Query: 121 ANRLTMKGKTNSDTIEASVRILTALFPPLLLELYRMLVSPIAGGKVAAIMVARVTALTCQ 180
           ANRLTMKGK+NSDTIEASVRIL ALFPPLLLELYRMLVSPIAGGKVAAIMVARVTALTCQ
Sbjct: 121 ANRLTMKGKSNSDTIEASVRILIALFPPLLLELYRMLVSPIAGGKVAAIMVARVTALTCQ 180

Query: 181 WLMGTCTVNSIDLPDGSSCQSGVFVEKCKYLEESKCIGICINTCKFPTQSFFKEQMGVPL 240
           WLMGTCTVNSI+LPDGSSCQSGVFVEKCKYLEESKCIGIC+NTCKFPTQSFFK QMG+PL
Sbjct: 181 WLMGTCTVNSIELPDGSSCQSGVFVEKCKYLEESKCIGICLNTCKFPTQSFFKNQMGIPL 222

Query: 241 LMEPNFNDYSCQ 251
           LMEPNFNDYSCQ
Sbjct: 241 LMEPNFNDYSCQ 222

BLAST of Lag0002655 vs. NCBI nr
Match: KAG6593243.1 (Beta-carotene isomerase D27, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 383.6 bits (984), Expect = 1.4e-102
Identity = 199/252 (78.97%), Postives = 208/252 (82.54%), Query Frame = 0

Query: 1   MKPLA-LLHPPA-ISSAPLHFSSKSHLRNRHFNFSCSALQSQSVRFQSSPRRLAIQLQLN 60
           MKP+A LLHPPA I SA L FSSKSHLRNR  +FSC A+QS+S                 
Sbjct: 1   MKPVALLLHPPATIPSAALRFSSKSHLRNRPLSFSCFAVQSEST---------------- 60

Query: 61  SVSPFSSSCLHPYIKGETSSKPEYKPGILDDFFLNLFRSKMVQEVGWDSEKPGYDGLIEV 120
                         K E SSKPEYKPG+LDDFFLNLFRSKMVQEVGWDSEKPGYDGLIEV
Sbjct: 61  --------------KVEVSSKPEYKPGMLDDFFLNLFRSKMVQEVGWDSEKPGYDGLIEV 120

Query: 121 ANRLTMKGKTNSDTIEASVRILTALFPPLLLELYRMLVSPIAGGKVAAIMVARVTALTCQ 180
           ANRLTMKGK+NSDTIEASVRIL ALFPPLLLELYRMLVSPIAGGKVAAIMVARVTA TCQ
Sbjct: 121 ANRLTMKGKSNSDTIEASVRILIALFPPLLLELYRMLVSPIAGGKVAAIMVARVTAFTCQ 180

Query: 181 WLMGTCTVNSIDLPDGSSCQSGVFVEKCKYLEESKCIGICINTCKFPTQSFFKEQMGVPL 240
           WLMGTCTVNSI+LPDGSSCQSGVFVEKCKYLEESKCIGIC+NTCKFPTQSFFK QMG+PL
Sbjct: 181 WLMGTCTVNSIELPDGSSCQSGVFVEKCKYLEESKCIGICLNTCKFPTQSFFKNQMGIPL 222

Query: 241 LMEPNFNDYSCQ 251
           LMEPNFNDYSCQ
Sbjct: 241 LMEPNFNDYSCQ 222

BLAST of Lag0002655 vs. NCBI nr
Match: XP_023514967.1 (beta-carotene isomerase D27, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 382.9 bits (982), Expect = 2.3e-102
Identity = 199/252 (78.97%), Postives = 207/252 (82.14%), Query Frame = 0

Query: 1   MKPLA-LLHPPA-ISSAPLHFSSKSHLRNRHFNFSCSALQSQSVRFQSSPRRLAIQLQLN 60
           MKP+A LLHPPA ISSA L FSSKSHLR R  N SC A+QS+S                 
Sbjct: 1   MKPVALLLHPPATISSAALRFSSKSHLRKRPLNISCFAVQSEST---------------- 60

Query: 61  SVSPFSSSCLHPYIKGETSSKPEYKPGILDDFFLNLFRSKMVQEVGWDSEKPGYDGLIEV 120
                         K E SSKPEYKPG+LDDFFLNLFRSKMVQEVGWDSEKPGYDGLIEV
Sbjct: 61  --------------KVEVSSKPEYKPGMLDDFFLNLFRSKMVQEVGWDSEKPGYDGLIEV 120

Query: 121 ANRLTMKGKTNSDTIEASVRILTALFPPLLLELYRMLVSPIAGGKVAAIMVARVTALTCQ 180
           ANRLTMKGK+NSDTIEASVRIL ALFPPLLLELYRMLVSPIAGGKVAAIMVARVTA TCQ
Sbjct: 121 ANRLTMKGKSNSDTIEASVRILIALFPPLLLELYRMLVSPIAGGKVAAIMVARVTAFTCQ 180

Query: 181 WLMGTCTVNSIDLPDGSSCQSGVFVEKCKYLEESKCIGICINTCKFPTQSFFKEQMGVPL 240
           WLMGTCTVNSI+LPDGSSCQSGVFVEKCKYLEESKCIGIC+NTCKFPTQSFFK QMG+PL
Sbjct: 181 WLMGTCTVNSIELPDGSSCQSGVFVEKCKYLEESKCIGICLNTCKFPTQSFFKNQMGIPL 222

Query: 241 LMEPNFNDYSCQ 251
           LMEPNFNDYSCQ
Sbjct: 241 LMEPNFNDYSCQ 222

BLAST of Lag0002655 vs. NCBI nr
Match: XP_022959652.1 (beta-carotene isomerase D27, chloroplastic [Cucurbita moschata])

HSP 1 Score: 381.3 bits (978), Expect = 6.8e-102
Identity = 198/252 (78.57%), Postives = 206/252 (81.75%), Query Frame = 0

Query: 1   MKPLA-LLHPPA-ISSAPLHFSSKSHLRNRHFNFSCSALQSQSVRFQSSPRRLAIQLQLN 60
           MKP+  LLHPPA ISSA L FSSKSHLR R  N SC A+QS+S                 
Sbjct: 1   MKPVVLLLHPPATISSAALRFSSKSHLRKRPLNISCFAVQSEST---------------- 60

Query: 61  SVSPFSSSCLHPYIKGETSSKPEYKPGILDDFFLNLFRSKMVQEVGWDSEKPGYDGLIEV 120
                         K E SSKPEYKPG+LDDFFLNLFRSKMVQEVGWDSEKPGYDGLIEV
Sbjct: 61  --------------KVEVSSKPEYKPGMLDDFFLNLFRSKMVQEVGWDSEKPGYDGLIEV 120

Query: 121 ANRLTMKGKTNSDTIEASVRILTALFPPLLLELYRMLVSPIAGGKVAAIMVARVTALTCQ 180
           ANRLTMKGK+NSDTIEASVRIL ALFPPLLLELYRMLVSPIAGGKVAAIMVARVTA TCQ
Sbjct: 121 ANRLTMKGKSNSDTIEASVRILIALFPPLLLELYRMLVSPIAGGKVAAIMVARVTAFTCQ 180

Query: 181 WLMGTCTVNSIDLPDGSSCQSGVFVEKCKYLEESKCIGICINTCKFPTQSFFKEQMGVPL 240
           WLMGTCTVNSI+LPDGSSCQSGVFVEKCKYLEESKCIGIC+NTCKFPTQSFFK QMG+PL
Sbjct: 181 WLMGTCTVNSIELPDGSSCQSGVFVEKCKYLEESKCIGICLNTCKFPTQSFFKNQMGIPL 222

Query: 241 LMEPNFNDYSCQ 251
           LMEPNFNDYSCQ
Sbjct: 241 LMEPNFNDYSCQ 222

BLAST of Lag0002655 vs. ExPASy Swiss-Prot
Match: Q7XA78 (Beta-carotene isomerase D27, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=D27 PE=1 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 8.0e-21
Identity = 66/213 (30.99%), Postives = 111/213 (52.11%), Query Frame = 0

Query: 58  NSVSPFSSSCLHPYIKGETSSKPE---------YKPGILDDFFLNL---FRSKMVQE--- 117
           +S+SP  + C  P   G+  +  E             I D FF  +   + SK +Q+   
Sbjct: 30  SSISP--TLCSKPVYSGKLKAAKETARIETSNTKNASIEDSFFSKIAINYLSKNLQDAAG 89

Query: 118 VGWDSEKPGYDGLIEVANRLTMKGKTNSDTIEASVRILTAL---FPPLLLELYRMLVSPI 177
           +   S+   YD L++ A R++     N DT +    +L++L    P ++  L +M   P 
Sbjct: 90  ISSSSKSTDYDRLVDTATRVS----RNFDTKQQHEFVLSSLDRALPTVISSLIKMAFPP- 149

Query: 178 AGGKVAAIMVARVTALTCQWLMGTCTVNSIDLPDGSSCQSGVFVEKCKYLEESKCIGICI 237
              KV+  + A  T ++  WL+G   V   ++ +G   +S V++EKC++LE+S C+G+C 
Sbjct: 150 --SKVSRELFALFTTISFAWLVGPSEVRETEV-NGRKEKSVVYIEKCRFLEQSNCVGMCT 209

Query: 238 NTCKFPTQSFFKEQMGVPLLMEPNFNDYSCQCM 253
           + CK P+Q F K  +G+P+ MEP+FND SC+ M
Sbjct: 210 HICKIPSQIFIKNSLGMPIYMEPDFNDLSCKMM 232

BLAST of Lag0002655 vs. ExPASy Swiss-Prot
Match: R4HZ96 (Beta-carotene isomerase D27, chloroplastic OS=Medicago truncatula OX=3880 GN=D27 PE=1 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 8.8e-20
Identity = 61/183 (33.33%), Postives = 94/183 (51.37%), Query Frame = 0

Query: 72  IKGETSSKPE-YKPGILDDFFLNLFRSKMVQEVGWDSEKPGYDGLIEVANRLTMKGKTNS 131
           I  ET  K   YK    D   +N     +    G  + K G+D L+E A   + K     
Sbjct: 42  ISEETLRKTNVYKDNWFDKLAINHLSKSVQAATGISNNKSGFDSLVEAATVASQK----F 101

Query: 132 DTIEASVRILTAL---FPPLLLELYRMLVSPIAGGKVAAIMVARVTALTCQWLMGTCTVN 191
           +T +    IL AL   FP  +L + R ++ P    K+A    A  T +   WL+G   V 
Sbjct: 102 NTTQQQGIILDALDRAFPKPILSVIRRVMPP---SKLAREYFAVFTTIFFAWLLGPSEVR 161

Query: 192 SIDLPDGSSCQSGVFVEKCKYLEESKCIGICINTCKFPTQSFFKEQMGVPLLMEPNFNDY 251
             ++ +G   ++ V ++KC++LEE+ C+G+CIN CK P+Q F K+  G+P+ M PNF+D 
Sbjct: 162 ESEI-NGRREKNIVHIKKCRFLEETNCVGMCINLCKMPSQLFIKDSFGMPVNMVPNFDDM 216

BLAST of Lag0002655 vs. ExPASy Swiss-Prot
Match: C7AU21 (Beta-carotene isomerase D27, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=D27 PE=1 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 1.8e-17
Identity = 53/220 (24.09%), Postives = 104/220 (47.27%), Query Frame = 0

Query: 34  CSALQSQSVRFQSSPRRLAIQLQLNSVSPFSSSCLHPYIKGETSSKPE---YKPGILDDF 93
           CS +++   R Q +P                ++ +   ++ ET++ P    Y+    D  
Sbjct: 36  CSTVRAVMARPQEAPASA-------PAKKTETAAMMSTVQTETAAAPPATVYRDSWFDKL 95

Query: 94  FLNLFRSKMVQEVGWDSEKPGYDGLIEVANRLTMKGKTNSDTIEASVRILTALFPPLLLE 153
            +      + +  G  +EK GY+ LI+ A  ++     +  + E   + L    P  +L 
Sbjct: 96  AIGYLSRNLQEASGLKNEKDGYESLIDAALAISRIFSLDKQS-EIVTQALERALPSYILT 155

Query: 154 LYRMLVSPIAGGKVAAIMVARVTALTCQWLMGTCTVNSIDLPDGSSCQSGVFVEKCKYLE 213
           + ++++ P    + +    A  T +   WL+G C V   ++ +G   ++ V++ KC++LE
Sbjct: 156 MIKVMMPP---SRFSREYFAAFTTIFFPWLVGPCEVMESEV-EGRKEKNVVYIPKCRFLE 215

Query: 214 ESKCIGICINTCKFPTQSFFKEQMGVPLLMEPNFNDYSCQ 251
            + C+G+C N CK P Q F ++ +G+ + M PNF D SC+
Sbjct: 216 STNCVGMCTNLCKIPCQKFIQDSLGMKVYMSPNFEDMSCE 243

BLAST of Lag0002655 vs. ExPASy TrEMBL
Match: A0A6J1KWU1 (beta-carotene isomerase D27, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111497865 PE=4 SV=1)

HSP 1 Score: 385.6 bits (989), Expect = 1.7e-103
Identity = 200/252 (79.37%), Postives = 209/252 (82.94%), Query Frame = 0

Query: 1   MKPLA-LLHPPA-ISSAPLHFSSKSHLRNRHFNFSCSALQSQSVRFQSSPRRLAIQLQLN 60
           MKP+A LLHPPA ISSA L FSSKSHLR R  NFSC A+QS+S                 
Sbjct: 1   MKPVALLLHPPATISSAALRFSSKSHLRKRPLNFSCFAVQSEST---------------- 60

Query: 61  SVSPFSSSCLHPYIKGETSSKPEYKPGILDDFFLNLFRSKMVQEVGWDSEKPGYDGLIEV 120
                         K E SSKP+YKPG+LDDFFLNLFRSKMVQEVGWDSEKPGYDGLIEV
Sbjct: 61  --------------KVEVSSKPDYKPGMLDDFFLNLFRSKMVQEVGWDSEKPGYDGLIEV 120

Query: 121 ANRLTMKGKTNSDTIEASVRILTALFPPLLLELYRMLVSPIAGGKVAAIMVARVTALTCQ 180
           ANRLTMKGK+NSDTIEASVRIL ALFPPLLLELYRMLVSPIAGGKVAAIMVARVTALTCQ
Sbjct: 121 ANRLTMKGKSNSDTIEASVRILIALFPPLLLELYRMLVSPIAGGKVAAIMVARVTALTCQ 180

Query: 181 WLMGTCTVNSIDLPDGSSCQSGVFVEKCKYLEESKCIGICINTCKFPTQSFFKEQMGVPL 240
           WLMGTCTVNSI+LPDGSSCQSGVFVEKCKYLEESKCIGIC+NTCKFPTQSFFK QMG+PL
Sbjct: 181 WLMGTCTVNSIELPDGSSCQSGVFVEKCKYLEESKCIGICLNTCKFPTQSFFKNQMGIPL 222

Query: 241 LMEPNFNDYSCQ 251
           LMEPNFNDYSCQ
Sbjct: 241 LMEPNFNDYSCQ 222

BLAST of Lag0002655 vs. ExPASy TrEMBL
Match: A0A6J1H551 (beta-carotene isomerase D27, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111460668 PE=4 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 3.3e-102
Identity = 198/252 (78.57%), Postives = 206/252 (81.75%), Query Frame = 0

Query: 1   MKPLA-LLHPPA-ISSAPLHFSSKSHLRNRHFNFSCSALQSQSVRFQSSPRRLAIQLQLN 60
           MKP+  LLHPPA ISSA L FSSKSHLR R  N SC A+QS+S                 
Sbjct: 1   MKPVVLLLHPPATISSAALRFSSKSHLRKRPLNISCFAVQSEST---------------- 60

Query: 61  SVSPFSSSCLHPYIKGETSSKPEYKPGILDDFFLNLFRSKMVQEVGWDSEKPGYDGLIEV 120
                         K E SSKPEYKPG+LDDFFLNLFRSKMVQEVGWDSEKPGYDGLIEV
Sbjct: 61  --------------KVEVSSKPEYKPGMLDDFFLNLFRSKMVQEVGWDSEKPGYDGLIEV 120

Query: 121 ANRLTMKGKTNSDTIEASVRILTALFPPLLLELYRMLVSPIAGGKVAAIMVARVTALTCQ 180
           ANRLTMKGK+NSDTIEASVRIL ALFPPLLLELYRMLVSPIAGGKVAAIMVARVTA TCQ
Sbjct: 121 ANRLTMKGKSNSDTIEASVRILIALFPPLLLELYRMLVSPIAGGKVAAIMVARVTAFTCQ 180

Query: 181 WLMGTCTVNSIDLPDGSSCQSGVFVEKCKYLEESKCIGICINTCKFPTQSFFKEQMGVPL 240
           WLMGTCTVNSI+LPDGSSCQSGVFVEKCKYLEESKCIGIC+NTCKFPTQSFFK QMG+PL
Sbjct: 181 WLMGTCTVNSIELPDGSSCQSGVFVEKCKYLEESKCIGICLNTCKFPTQSFFKNQMGIPL 222

Query: 241 LMEPNFNDYSCQ 251
           LMEPNFNDYSCQ
Sbjct: 241 LMEPNFNDYSCQ 222

BLAST of Lag0002655 vs. ExPASy TrEMBL
Match: A0A1S3BRJ4 (beta-carotene isomerase D27, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492751 PE=4 SV=1)

HSP 1 Score: 379.0 bits (972), Expect = 1.6e-101
Identity = 199/252 (78.97%), Postives = 208/252 (82.54%), Query Frame = 0

Query: 1   MKPLA-LLHPPAISSAPLHF-SSKSHLRNRHFNFSCSALQSQSVRFQSSPRRLAIQLQLN 60
           MKPLA LLHPP ISSAPL F SSKSHLRNR F  SCSALQS S                 
Sbjct: 1   MKPLALLLHPPTISSAPLRFNSSKSHLRNRPFILSCSALQSGS----------------- 60

Query: 61  SVSPFSSSCLHPYIKGETSSKPEYKPGILDDFFLNLFRSKMVQEVGWDSEKPGYDGLIEV 120
                        IK  TSSK EYKPGILDDFFLN+FRSKMVQEVGWDSEKPGY GLIEV
Sbjct: 61  -------------IKDGTSSKSEYKPGILDDFFLNVFRSKMVQEVGWDSEKPGYGGLIEV 120

Query: 121 ANRLTMKGKTNSDTIEASVRILTALFPPLLLELYRMLVSPIAGGKVAAIMVARVTALTCQ 180
           A+RLTM G+TNS+TIEASVRILTALFPPLLL+LYR+LVSPIAGGKVAAIMVARVTALTCQ
Sbjct: 121 ASRLTMTGRTNSETIEASVRILTALFPPLLLKLYRILVSPIAGGKVAAIMVARVTALTCQ 180

Query: 181 WLMGTCTVNSIDLPDGSSCQSGVFVEKCKYLEESKCIGICINTCKFPTQSFFKEQMGVPL 240
           WLMGTCTVNSI+LPDGSSCQSGVFVEKCKYLEESKCIGICINTCK PTQSFFK+QMG+PL
Sbjct: 181 WLMGTCTVNSIELPDGSSCQSGVFVEKCKYLEESKCIGICINTCKLPTQSFFKDQMGIPL 222

Query: 241 LMEPNFNDYSCQ 251
           LMEPNFNDYSCQ
Sbjct: 241 LMEPNFNDYSCQ 222

BLAST of Lag0002655 vs. ExPASy TrEMBL
Match: A0A5D3D2R7 (Beta-carotene isomerase D27 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G001770 PE=4 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 2.8e-101
Identity = 198/252 (78.57%), Postives = 208/252 (82.54%), Query Frame = 0

Query: 1   MKPLA-LLHPPAISSAPLHF-SSKSHLRNRHFNFSCSALQSQSVRFQSSPRRLAIQLQLN 60
           MKP+A LLHPP ISSAPL F SSKSHLRNR F  SCSALQS S                 
Sbjct: 1   MKPMALLLHPPTISSAPLRFNSSKSHLRNRPFILSCSALQSGS----------------- 60

Query: 61  SVSPFSSSCLHPYIKGETSSKPEYKPGILDDFFLNLFRSKMVQEVGWDSEKPGYDGLIEV 120
                        IK  TSSK EYKPGILDDFFLN+FRSKMVQEVGWDSEKPGY GLIEV
Sbjct: 61  -------------IKDGTSSKSEYKPGILDDFFLNVFRSKMVQEVGWDSEKPGYGGLIEV 120

Query: 121 ANRLTMKGKTNSDTIEASVRILTALFPPLLLELYRMLVSPIAGGKVAAIMVARVTALTCQ 180
           A+RLTM G+TNS+TIEASVRILTALFPPLLL+LYR+LVSPIAGGKVAAIMVARVTALTCQ
Sbjct: 121 ASRLTMTGRTNSETIEASVRILTALFPPLLLKLYRILVSPIAGGKVAAIMVARVTALTCQ 180

Query: 181 WLMGTCTVNSIDLPDGSSCQSGVFVEKCKYLEESKCIGICINTCKFPTQSFFKEQMGVPL 240
           WLMGTCTVNSI+LPDGSSCQSGVFVEKCKYLEESKCIGICINTCK PTQSFFK+QMG+PL
Sbjct: 181 WLMGTCTVNSIELPDGSSCQSGVFVEKCKYLEESKCIGICINTCKLPTQSFFKDQMGIPL 222

Query: 241 LMEPNFNDYSCQ 251
           LMEPNFNDYSCQ
Sbjct: 241 LMEPNFNDYSCQ 222

BLAST of Lag0002655 vs. ExPASy TrEMBL
Match: A0A6J1DMD9 (beta-carotene isomerase D27, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111022359 PE=4 SV=1)

HSP 1 Score: 377.5 bits (968), Expect = 4.7e-101
Identity = 194/251 (77.29%), Postives = 205/251 (81.67%), Query Frame = 0

Query: 1   MKPLALLHPPAISSAPLHF-SSKSHLRNRHFNFSCSALQSQSVRFQSSPRRLAIQLQLNS 60
           MKPLA+L PPAISS PL F SSKSHLRN   N SCSALQS+S+                 
Sbjct: 1   MKPLAVLRPPAISSPPLRFSSSKSHLRNHSINTSCSALQSESI----------------- 60

Query: 61  VSPFSSSCLHPYIKGETSSKPEYKPGILDDFFLNLFRSKMVQEVGWDSEKPGYDGLIEVA 120
                       IK   S K EYKPGILDDFFLNLFRSKMVQEVGWDS+KPGY GL+EVA
Sbjct: 61  ------------IKDGVSPKTEYKPGILDDFFLNLFRSKMVQEVGWDSDKPGYGGLMEVA 120

Query: 121 NRLTMKGKTNSDTIEASVRILTALFPPLLLELYRMLVSPIAGGKVAAIMVARVTALTCQW 180
           NRLTMKGKTNSDT+EASVRILT+LFPPLLLELYRMLVSPIAGGKVAAIMVARVTALTCQW
Sbjct: 121 NRLTMKGKTNSDTVEASVRILTSLFPPLLLELYRMLVSPIAGGKVAAIMVARVTALTCQW 180

Query: 181 LMGTCTVNSIDLPDGSSCQSGVFVEKCKYLEESKCIGICINTCKFPTQSFFKEQMGVPLL 240
           LMGT TVNSI+LPDGSSCQSG+FVEKCKYLEESKCIGICINTCK PTQ+FFK+QMGVPLL
Sbjct: 181 LMGTSTVNSIELPDGSSCQSGIFVEKCKYLEESKCIGICINTCKLPTQNFFKDQMGVPLL 222

Query: 241 MEPNFNDYSCQ 251
           MEPNF DYSCQ
Sbjct: 241 MEPNFTDYSCQ 222

BLAST of Lag0002655 vs. TAIR 10
Match: AT4G01995.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G64680.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 264.6 bits (675), Expect = 8.6e-71
Identity = 123/176 (69.89%), Postives = 149/176 (84.66%), Query Frame = 0

Query: 75  ETSSKPEYKPGILDDFFLNLFRSKMVQEVGWDSEKPGYDGLIEVANRLTMKGKTNSDTIE 134
           E + K EYKPG LDDFF+  FR+K+V+EVG DSEKPGY GLIE+   L +KG+T S+T +
Sbjct: 45  EGAPKLEYKPGPLDDFFMQSFRNKLVEEVGSDSEKPGYVGLIELVKLLLLKGRTRSETSD 104

Query: 135 ASVRILTALFPPLLLELYRMLVSPIAGGKVAAIMVARVTALTCQWLMGTCTVNSIDLPDG 194
           A+VRIL +LFPPL+LELY++L++PIA GK+AA+MVARVT LTCQWLMG   VN IDLP+G
Sbjct: 105 AAVRILKSLFPPLILELYKLLIAPIAQGKLAALMVARVTVLTCQWLMGPSKVNIIDLPNG 164

Query: 195 SSCQSGVFVEKCKYLEESKCIGICINTCKFPTQSFFKEQMGVPLLMEPNFNDYSCQ 251
            S  SGVFVEKC+YLEESKC+G+CINTCK PTQ+FFK+ MGVPL+MEPNF DYSCQ
Sbjct: 165 ESWDSGVFVEKCQYLEESKCVGVCINTCKLPTQTFFKDYMGVPLVMEPNFKDYSCQ 220

BLAST of Lag0002655 vs. TAIR 10
Match: AT1G64680.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G03055.1); Has 146 Blast hits to 146 proteins in 26 species: Archae - 0; Bacteria - 6; Metazoa - 0; Fungi - 0; Plants - 125; Viruses - 0; Other Eukaryotes - 15 (source: NCBI BLink). )

HSP 1 Score: 112.5 bits (280), Expect = 5.5e-25
Identity = 65/183 (35.52%), Postives = 101/183 (55.19%), Query Frame = 0

Query: 79  KPEYKPGILDDFFLNLFRSKM----------VQEVG-WDSEKPGYDGLIEVANRLTMKGK 138
           K  Y+ G+++  F+ LF  KM           +E G W+ +   Y+  +EV+ R+ M+G+
Sbjct: 36  KTRYEDGLVERVFMGLFARKMDKFGSKKKKDTKEKGFWEYD---YESFVEVSKRV-MQGR 95

Query: 139 TNSDTIEASVRILTALFPPLLLELYRMLVSPIAGGKVAAIMVARVTALTCQWLMGTCTVN 198
           +     EA   +L ++ PP   E +R L  P    K AA   A +T     WL+G   V 
Sbjct: 96  SRVQQQEAVREVLLSMLPPGAPEQFRKLFPPT---KWAAEFNAALTVPFFHWLVGPSQVI 155

Query: 199 SIDLPDGSSCQSGVFVEKCKYLEESKCIGICINTCKFPTQSFFKEQMGVPLLMEPNFNDY 251
            +++ +G   +SGV ++KC+YLE S C+G+C+N CK PTQ FF  + G+PL M PN+ D 
Sbjct: 156 EVEV-NGVKQRSGVRIKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNYEDM 210

BLAST of Lag0002655 vs. TAIR 10
Match: AT1G03055.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G64680.1); Has 143 Blast hits to 143 proteins in 26 species: Archae - 0; Bacteria - 6; Metazoa - 0; Fungi - 0; Plants - 122; Viruses - 0; Other Eukaryotes - 15 (source: NCBI BLink). )

HSP 1 Score: 102.4 bits (254), Expect = 5.7e-22
Identity = 66/213 (30.99%), Postives = 111/213 (52.11%), Query Frame = 0

Query: 58  NSVSPFSSSCLHPYIKGETSSKPE---------YKPGILDDFFLNL---FRSKMVQE--- 117
           +S+SP  + C  P   G+  +  E             I D FF  +   + SK +Q+   
Sbjct: 30  SSISP--TLCSKPVYSGKLKAAKETARIETSNTKNASIEDSFFSKIAINYLSKNLQDAAG 89

Query: 118 VGWDSEKPGYDGLIEVANRLTMKGKTNSDTIEASVRILTAL---FPPLLLELYRMLVSPI 177
           +   S+   YD L++ A R++     N DT +    +L++L    P ++  L +M   P 
Sbjct: 90  ISSSSKSTDYDRLVDTATRVS----RNFDTKQQHEFVLSSLDRALPTVISSLIKMAFPP- 149

Query: 178 AGGKVAAIMVARVTALTCQWLMGTCTVNSIDLPDGSSCQSGVFVEKCKYLEESKCIGICI 237
              KV+  + A  T ++  WL+G   V   ++ +G   +S V++EKC++LE+S C+G+C 
Sbjct: 150 --SKVSRELFALFTTISFAWLVGPSEVRETEV-NGRKEKSVVYIEKCRFLEQSNCVGMCT 209

Query: 238 NTCKFPTQSFFKEQMGVPLLMEPNFNDYSCQCM 253
           + CK P+Q F K  +G+P+ MEP+FND SC+ M
Sbjct: 210 HICKIPSQIFIKNSLGMPIYMEPDFNDLSCKMM 232