Lag0002579 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0002579
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	exopolygalacturonase-like
Location	chr4: 43932620 .. 43933827 (-)
RNA-Seq Expression	Lag0002579
Synteny	Lag0002579

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACAACGTCAAGAAGTTTTAGCCTCTTCCAAATCCTTCTCTTGGTATTTGTTTGGCAATGTTGTGCAATGGTGGCTGCCATTTTCAACAACGACCTCGGTATTGTGAATCTCGTCGGCGGGATTGCATTGACGGTAAATCAGCAACTTCCTACAGCCCCTCCACAGGCTCTTGGTATCGGAACTCTGACAGGCAGTGACGGTAGTGTTGCAGTAAATGACGTTAAGGCCACTATTGATCTGAATGGGAAGGGTGGTGCAGTTTTTGATGTTAGAAAGTATGAAGCTAAAACTGATGGAAAGACTGATAATGCACCGGCGATGATAATTAACCTCAATGAAATCACAGATTTTAAACTTTTAATTTCAATAATTATCTTTGAACTTTTTTTTTTGAAAAAAATTATCTTTGAACTTATAAAATTATTGCAATTTGAGCGATTATCATCTAAATATACAAAACCATTGATTTTTTTTTTTTTTTTTTTTTGAACCACAAAACCATTGAAATTACATCCCTACTGTTTTTAACTCCATTTGCTATTGGGTTTAAACGCAATAACTTTATAAGTTAGAACCAGTATAATTGCAACAATATTATTTAAAGAAAATTAAAAAAAAAATACTAATTATGAAGATTATAGAGCTTTTTTGAAAGTTGGCGGGATTAGATTTGTTACGATTGCATTATTGCTGCTCCATAAATTTTCAAAATTATTTTTCAATATTAAACTCTACCTTAATTAAAAAAAAAAAAATCTAAGATTTAAACTTAAATGGCTCAAAATTTAAATGGTAACAAGAATAATATTTACCAACTTAAAAAACAATCTCTACCTTTCTAATTTAACTAGCAACTTTTTTCTACTTTTCTAAAAAAAAAATAACTTTGATTTTCCACGTGGCAGGCATTTATGAAGACATGGATTGCAACATGTCGGAAGACATACGGTCCGACGAAGTTTTTGATCCCACGGGGCACATTTCTGGTGGGTCCGGTGACTTTTGCTGGTCCGTGCAAAAGCTTGCCGATCACCATCGAAATTCAGGGAACTGTAAAGGCCACAACCGATATCAGTGAATATTCTTCCCCTAAATGGTTCTCCATCGAACATATCATCGGCCTTATCCTCACCGGTTCTGGCGTCTTCGACGGCCAAGGCGCCTCGACCTGGTCCTATAATGACTACAAGAAAAATATTCACTGA

mRNA sequence

ATGACAACGTCAAGAAGTTTTAGCCTCTTCCAAATCCTTCTCTTGGTATTTGTTTGGCAATGTTGTGCAATGGTGGCTGCCATTTTCAACAACGACCTCGGTATTGTGAATCTCGTCGGCGGGATTGCATTGACGGTAAATCAGCAACTTCCTACAGCCCCTCCACAGGCTCTTGGTATCGGAACTCTGACAGGCAGTGACGGTAGTGTTGCAGTAAATGACGTTAAGGCCACTATTGATCTGAATGGGAAGGGTGGTGCAGTTTTTGATGCATTTATGAAGACATGGATTGCAACATGTCGGAAGACATACGGTCCGACGAAGTTTTTGATCCCACGGGGCACATTTCTGGTGGGTCCGGTGACTTTTGCTGGTCCGTGCAAAAGCTTGCCGATCACCATCGAAATTCAGGGAACTGTAAAGGCCACAACCGATATCAGTGAATATTCTTCCCCTAAATGGTTCTCCATCGAACATATCATCGGCCTTATCCTCACCGGTTCTGGCGTCTTCGACGGCCAAGGCGCCTCGACCTGGTCCTATAATGACTACAAGAAAAATATTCACTGA

Coding sequence (CDS)

ATGACAACGTCAAGAAGTTTTAGCCTCTTCCAAATCCTTCTCTTGGTATTTGTTTGGCAATGTTGTGCAATGGTGGCTGCCATTTTCAACAACGACCTCGGTATTGTGAATCTCGTCGGCGGGATTGCATTGACGGTAAATCAGCAACTTCCTACAGCCCCTCCACAGGCTCTTGGTATCGGAACTCTGACAGGCAGTGACGGTAGTGTTGCAGTAAATGACGTTAAGGCCACTATTGATCTGAATGGGAAGGGTGGTGCAGTTTTTGATGCATTTATGAAGACATGGATTGCAACATGTCGGAAGACATACGGTCCGACGAAGTTTTTGATCCCACGGGGCACATTTCTGGTGGGTCCGGTGACTTTTGCTGGTCCGTGCAAAAGCTTGCCGATCACCATCGAAATTCAGGGAACTGTAAAGGCCACAACCGATATCAGTGAATATTCTTCCCCTAAATGGTTCTCCATCGAACATATCATCGGCCTTATCCTCACCGGTTCTGGCGTCTTCGACGGCCAAGGCGCCTCGACCTGGTCCTATAATGACTACAAGAAAAATATTCACTGA

Protein sequence

MTTSRSFSLFQILLLVFVWQCCAMVAAIFNNDLGIVNLVGGIALTVNQQLPTAPPQALGIGTLTGSDGSVAVNDVKATIDLNGKGGAVFDAFMKTWIATCRKTYGPTKFLIPRGTFLVGPVTFAGPCKSLPITIEIQGTVKATTDISEYSSPKWFSIEHIIGLILTGSGVFDGQGASTWSYNDYKKNIH

Homology

[Close]

BLAST of Lag0002579 vs. NCBI nr
Match: XP_038896336.1 (exopolygalacturonase-like [Benincasa hispida])

HSP 1 Score: 249.6 bits (636), Expect = 2.2e-62
Identity = 131/203 (64.53%), Postives = 145/203 (71.43%), Query Frame = 0


Query: 3   TSRSFSLFQILLLVFVWQCCAMVAAIFNNDLGIVNLVGGIALTVNQQL--PTAPPQALGI 62
           T  S SLFQILLL F WQCCA VAAIF++  G  NLV G+  TVNQ L  P A P  LGI
Sbjct: 2   TMTSPSLFQILLLAFAWQCCAEVAAIFDDITGTANLVNGVVSTVNQPLLHPAAAPLPLGI 61

Query: 63  GTLTGSDGSVAVNDVKATIDLNGKGGAVFD----------------AFMKTWIATCRKTY 122
           GTL    G+  VND+KA +DLNG GG+VFD                AFM TWI  CR   
Sbjct: 62  GTLPDLGGT--VNDIKANVDLNGNGGSVFDVTKHGAKGDGKTDDAQAFMTTWIEACRNAE 121

Query: 123 GPTKFLIPRGTFLVGPVTFAGPCKSLPITIEIQGTVKATTDISEYSSPKWFSIEHIIGLI 182
           GP KFLIP+GTFLVGPVTFAGPCKS PIT+E QGTVKATTDI++YSSP+WFSIE I G I
Sbjct: 122 GPAKFLIPQGTFLVGPVTFAGPCKSFPITLENQGTVKATTDITQYSSPEWFSIEEITGFI 181

Query: 183 LTGSGVFDGQGASTWSYNDYKKN 188
           LTGSGVFDGQGA++W YND KKN
Sbjct: 182 LTGSGVFDGQGAASWPYNDCKKN 202

[Close]

BLAST of Lag0002579 vs. NCBI nr
Match: XP_038896337.1 (exopolygalacturonase clone GBGE184-like [Benincasa hispida])

HSP 1 Score: 247.3 bits (630), Expect = 1.1e-61
Identity = 131/201 (65.17%), Postives = 144/201 (71.64%), Query Frame = 0


Query: 5   RSFSLFQILLLVFVWQCCAMVAAIFNNDLGIVNLVGGIALTVNQQL--PTAPPQALGIGT 64
           +S SLFQILLLV  WQCCA VAAI ++  G  NLV GI   VNQ+L  P A P++LGIGT
Sbjct: 3   KSLSLFQILLLVLAWQCCAKVAAILDDATGASNLVDGIVSMVNQKLLHPAAAPRSLGIGT 62

Query: 65  LTGSDGSVAVNDVKATIDLNGKGGAVFD----------------AFMKTWIATCRKTYGP 124
           L    G+  VND+KA  DLNG GGAVFD                AFM TWIA CR T GP
Sbjct: 63  LPDIGGT--VNDIKANADLNGNGGAVFDVTKYGAKADGKTDDAQAFMTTWIAACRNTDGP 122

Query: 125 TKFLIPRGTFLVGPVTFAGPCKSLPITIEIQGTVKATTDISEYSSPKWFSIEHIIGLILT 184
            KFLIP+GTFLVGPVTFAGPCKS PIT+E QGTVKATTDI+EYSSP+WFSIE I G ILT
Sbjct: 123 AKFLIPQGTFLVGPVTFAGPCKSFPITLENQGTVKATTDITEYSSPEWFSIEDITGFILT 182

Query: 185 GSGVFDGQGASTWSYNDYKKN 188
           GSGVFDGQG + W YND K N
Sbjct: 183 GSGVFDGQGTAAWPYNDCKTN 201

[Close]

BLAST of Lag0002579 vs. NCBI nr
Match: XP_022991773.1 (exopolygalacturonase-like [Cucurbita maxima])

HSP 1 Score: 243.0 bits (619), Expect = 2.0e-60
Identity = 131/204 (64.22%), Postives = 141/204 (69.12%), Query Frame = 0


Query: 1   MTTSRSFSLFQILLLVFVWQCCAMVAAIFNNDLGIVNLVGGIALTVNQQ-LPTAPPQALG 60
           MTT+R+ SLFQ LLLV   QCCA VAA+F++  G  NL+ GI  T NQQ  P A P  LG
Sbjct: 1   MTTTRNLSLFQTLLLVLACQCCAEVAAVFDHVTGTANLIDGIVSTANQQHRPPAAPLPLG 60

Query: 61  IGTLTGSDGSVAVNDVKATIDLNGKGGAVFD----------------AFMKTWIATCRKT 120
           I T     GSVA+N  KA + L G G AVFD                AFM TWIA CR T
Sbjct: 61  IRTPANRRGSVAINGTKAAVYLKGNGNAVFDVKKYGAKANGKSDDAQAFMTTWIAACRNT 120

Query: 121 YGPTKFLIPRGTFLVGPVTFAGPCKSLPITIEIQGTVKATTDISEYSSPKWFSIEHIIGL 180
            GP KFLIP+G FLVGPVTFAGPCKS+PITIEIQGTVKATTDISEYSSP W SIE I GL
Sbjct: 121 TGPAKFLIPKGIFLVGPVTFAGPCKSMPITIEIQGTVKATTDISEYSSPDWISIEGITGL 180

Query: 181 ILTGSGVFDGQGASTWSYNDYKKN 188
           ILTGSGVFDGQGAS W YND K N
Sbjct: 181 ILTGSGVFDGQGASAWPYNDCKIN 204

[Close]

BLAST of Lag0002579 vs. NCBI nr
Match: KAG7014351.1 (Exopolygalacturonase-like 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 242.3 bits (617), Expect = 3.4e-60
Identity = 132/206 (64.08%), Postives = 142/206 (68.93%), Query Frame = 0


Query: 1   MTTSRSFSLFQILLLVFVWQCCAMVAAIFNNDLGIVNLVGGIALTVNQQ-LPTAPPQALG 60
           MTT+ + SLFQ LLLV   QCCA VAA+F++     N + GI  T NQQ  P APP  LG
Sbjct: 1   MTTTTNLSLFQTLLLVLACQCCAEVAAVFDHVKVAANPIDGIVSTANQQHRPPAPPLPLG 60

Query: 61  IGTLTGSDGSVAVNDVKATIDL--NGKGGAVFD----------------AFMKTWIATCR 120
           IGTL  S G VA+N  KA +DL  NG G AVFD                AFM TWIA CR
Sbjct: 61  IGTLANSRGRVAINSTKAAVDLKGNGNGNAVFDVKKYGAKANGKSDDAQAFMTTWIAACR 120

Query: 121 KTYGPTKFLIPRGTFLVGPVTFAGPCKSLPITIEIQGTVKATTDISEYSSPKWFSIEHII 180
            T GP KFLIP+GTFLVGPV FAGPC+S PITIEIQGTVKATTDISEYSSP W SIE I 
Sbjct: 121 NTTGPAKFLIPKGTFLVGPVVFAGPCQSRPITIEIQGTVKATTDISEYSSPDWISIEGIT 180

Query: 181 GLILTGSGVFDGQGASTWSYNDYKKN 188
           GLILTGSGVFDGQGAS W YND K N
Sbjct: 181 GLILTGSGVFDGQGASAWPYNDCKTN 206

[Close]

BLAST of Lag0002579 vs. NCBI nr
Match: KAG6575809.1 (Exopolygalacturonase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 242.3 bits (617), Expect = 3.4e-60
Identity = 132/206 (64.08%), Postives = 142/206 (68.93%), Query Frame = 0


Query: 1   MTTSRSFSLFQILLLVFVWQCCAMVAAIFNNDLGIVNLVGGIALTVNQQ-LPTAPPQALG 60
           MTT+ + SLFQ LLLV   QCCA VAA+F++     N + GI  T NQQ  P APP  LG
Sbjct: 1   MTTTTNLSLFQTLLLVLACQCCAEVAAVFDHVKVAANPIDGIVSTANQQHRPPAPPLPLG 60

Query: 61  IGTLTGSDGSVAVNDVKATIDL--NGKGGAVFD----------------AFMKTWIATCR 120
           IGTL  S G VA+N  KA +DL  NG G AVFD                AFM TWIA CR
Sbjct: 61  IGTLANSRGRVAINSTKAAVDLKGNGNGNAVFDVKKYGAKANGKSDDAQAFMTTWIAACR 120

Query: 121 KTYGPTKFLIPRGTFLVGPVTFAGPCKSLPITIEIQGTVKATTDISEYSSPKWFSIEHII 180
            T GP KFLIP+GTFLVGPV FAGPC+S PITIEIQGTVKATTDISEYSSP W SIE I 
Sbjct: 121 NTTGPAKFLIPKGTFLVGPVVFAGPCQSRPITIEIQGTVKATTDISEYSSPDWISIEGIT 180

Query: 181 GLILTGSGVFDGQGASTWSYNDYKKN 188
           GLILTGSGVFDGQGAS W YND K N
Sbjct: 181 GLILTGSGVFDGQGASAWPYNDCKTN 206

[Close]

BLAST of Lag0002579 vs. ExPASy Swiss-Prot
Match: P49062 (Exopolygalacturonase clone GBGE184 OS=Arabidopsis thaliana OX=3702 GN=PGA3 PE=2 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 2.8e-20
Identity = 55/135 (40.74%), Postives = 74/135 (54.81%), Query Frame = 0


Query: 61  GTLTGSDGSVAVNDVKATIDLNGKGGAV-------FDAFMKTWIATCRKTYGPTKFLIPR 120
           G   G    +A N    T+    K GAV       F AF+ TWI  C     P   L+P+
Sbjct: 33  GEYLGGRRGLAANSGNPTVYDITKFGAVGDGSTNTFKAFLNTWIQVCDSPV-PATLLVPK 92

Query: 121 GTFLVGPVTFAGPCKSLPITIEIQGTVKATTDISEYSSPKWFSIEHIIGLILTGSGVFDG 180
           GTFL GPV FAGPCKS  +T+ + GT+ ATT  S Y++P+WF  E +  L+LTG+G F G
Sbjct: 93  GTFLAGPVIFAGPCKS-KVTVNVIGTIIATT--SGYATPEWFLFERVDNLVLTGTGTFHG 152

Query: 181 QGASTWSYNDYKKNI 189
           +G + W  +   K +
Sbjct: 153 KGEAVWKADGCGKKV 163

[Close]

BLAST of Lag0002579 vs. ExPASy Swiss-Prot
Match: Q6H9K0 (Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 9.8e-18
Identity = 46/109 (42.20%), Postives = 61/109 (55.96%), Query Frame = 0


Query: 80  DLNGKG-GAVFDAFMKTWIATCRKTYGPTKFLIPRGTFLVGPVTFAGPCKSLPITIEIQG 139
           D   KG G +  A MK W A C  + GP+  LIP+G + +G V   GPCK   I  +I G
Sbjct: 14  DYGAKGAGDISQAVMKAWKAAC-ASQGPSTVLIPKGNYNMGEVAMQGPCKGSKIGFQIDG 73

Query: 140 TVKATTDISEYSSPKWFSIEHIIGLILTGSGVFDGQGASTWSYNDYKKN 188
            VKA  D S++ S  W S   I GL ++G+G  DGQG + W+ N+  KN
Sbjct: 74  VVKAPADPSKFKSDGWVSFYRIDGLTVSGTGTLDGQGQTAWAKNNCDKN 121

[Close]

BLAST of Lag0002579 vs. ExPASy Swiss-Prot
Match: P24548 (Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 7.8e-15
Identity = 41/97 (42.27%), Postives = 53/97 (54.64%), Query Frame = 0


Query: 91  AFMKTWIATCRKTYGPTKFLIPRGTFLVGPVTFAGPCKSLPITIEIQGTVKATTDISEYS 150
           A    W   C     P+  L+P+G F VG +T  GPCKS  I +++QGT+KA  D S+  
Sbjct: 5   ALTTAWKEACASA-SPSTILVPKGNFAVGLITLEGPCKS-SIGLQLQGTLKAPADPSKIK 64

Query: 151 SPKWFSIEHIIGLILTGSGVFDGQGASTWSYNDYKKN 188
              W ++  I  L + G GVFDGQG S W  ND  KN
Sbjct: 65  GLGWINLNKIDLLTIFGGGVFDGQGKSAWVQNDCHKN 99

[Close]

BLAST of Lag0002579 vs. ExPASy Swiss-Prot
Match: Q39766 (Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 3.6e-12
Identity = 33/91 (36.26%), Postives = 50/91 (54.95%), Query Frame = 0


Query: 92  FMKTWIATCRKTYGPTKFLIPRGTFLVGPVTFAGPCKSLPITIEIQGTVKATTDISEYSS 151
           F+  W   C  +  P+  +IP+GT+L+  V   GPCK+ PI I +QGT++A  D S +  
Sbjct: 49  FLDAWKEAC-ASVTPSTVVIPKGTYLLSKVNLEGPCKA-PIEINVQGTIQAPADPSAFKD 108

Query: 152 PKWFSIEHIIGLILTGSGVFDGQGASTWSYN 183
           P W     +    + G G+FDGQG+  +  N
Sbjct: 109 PNWVRFYSVENFKMFGGGIFDGQGSIAYEKN 137

[Close]

BLAST of Lag0002579 vs. ExPASy Swiss-Prot
Match: Q39786 (Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 3.6e-12
Identity = 33/91 (36.26%), Postives = 50/91 (54.95%), Query Frame = 0


Query: 92  FMKTWIATCRKTYGPTKFLIPRGTFLVGPVTFAGPCKSLPITIEIQGTVKATTDISEYSS 151
           F+  W   C  +  P+  +IP+GT+L+  V   GPCK+ PI I +QGT++A  D S +  
Sbjct: 49  FLDAWKEAC-ASVTPSTVVIPKGTYLLSKVNLEGPCKA-PIEINVQGTIQAPADPSAFKD 108

Query: 152 PKWFSIEHIIGLILTGSGVFDGQGASTWSYN 183
           P W     +    + G G+FDGQG+  +  N
Sbjct: 109 PNWVRFYSVENFKMFGGGIFDGQGSIAYEKN 137

The following BLAST results are available for this feature:

BLAST of Lag0002579 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038896336.1	2.2e-62	64.53	exopolygalacturonase-like [Benincasa hispida]	[more]
XP_038896337.1	1.1e-61	65.17	exopolygalacturonase clone GBGE184-like [Benincasa hispida]	[more]
XP_022991773.1	2.0e-60	64.22	exopolygalacturonase-like [Cucurbita maxima]	[more]
KAG7014351.1	3.4e-60	64.08	Exopolygalacturonase-like 4, partial [Cucurbita argyrosperma subsp. argyrosperma...	[more]
KAG6575809.1	3.4e-60	64.08	Exopolygalacturonase, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Lag0002579 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P49062	2.8e-20	40.74	Exopolygalacturonase clone GBGE184 OS=Arabidopsis thaliana OX=3702 GN=PGA3 PE=2 ...	[more]
Q6H9K0	9.8e-18	42.20	Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 S...	[more]
P24548	7.8e-15	42.27	Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1	[more]
Q39766	3.6e-12	36.26	Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1	[more]
Q39786	3.6e-12	36.26	Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 105..183 e-value: 8.1E-12 score: 44.8
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 82..189 e-value: 4.1E-15 score: 57.6
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 3..187
None	No IPR available	PANTHER	PTHR31375:SF158	POLYGALACTURONASE	coord: 3..187
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 89..186

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0002579.1	Lag0002579.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0016226	iron-sulfur cluster assembly
biological_process	GO:0097428	protein maturation by iron-sulfur cluster transfer
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0051539	4 iron, 4 sulfur cluster binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004650	polygalacturonase activity