Lag0001992 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0001992
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Alpha/beta-Hydrolases superfamily protein
Location	chr4: 37990592 .. 37995541 (+)
RNA-Seq Expression	Lag0001992
Synteny	Lag0001992

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCAGTCGAACTGCGAGGCGTCTTCTTCACAACTTCCGTAAGTGAAATTTTCTTTTACTTCTTTATTGATTTTTTACTGCCTCATGAATGTCTGAGAACTTGTGTCTGCTTCTTGATTGTGGAATCAATGCGAGTTTAATGCCAGTTTGGTGTCTTTTGCGGTTTTCTTTATGTGAATCTTGATACTCTATGAAAATGTGATGATGAATTGTGCGTTCTTTCTGTTACGCATTCTATATATGCTGCATTGGGAAGAAAAATCGATCTTGATTTTCGTAATTTTGTTTTGTTCAAGCTTTTAGTTAGTTAGGGATTGATAAGCTGTTGAAATTGTTTGTGGAAGGCAATGTTTAGAGATTTGCTTAATCTAATTCATTATCTTATCTGATGTCGAATTCGATTGGGTTCTTTAATTTTTTAAGTTTTTCCAAACAAGGAAAATGGAAGTAGATATGAATTGAATTATAAATCTACAATTGATATAATTCAGTCTTAAATGGATTTTTGAAGGCGAATGATCCTTCAGCTATTTTTGTATCTTGAGTAGGTTTGGTCCGTGCATAACTGTTAATTAATACGGAAAGGTGGTTGGTTAGTATGCCTAACTTGTAAAGGGTATTATTCTGATTTGGTCAATACTATTACTATTCAACTACCTAATTGTGAGGGTTTAGGGTTTAGGGTTTCAGGGGTTTTAGTTATTATAGTTTACATTGATGAATGATTCTGGTCACACTTCTATCTTTAATCGTAAGTTATAAAATGGCTTTCTCGGGTATCAATTTAAGGTTTCATGGCTATAATGTAGATGTTTTTTCATATGGGGTATGATATTTACATGGATTGTTTTTTCTTCAACCCTGCAGATTATGCCAGAAGCTTTCTCAGGAAACAGGAGTGGCAGGCTAGCTCTGTAACTGTGAATAGTGTTCTTTTGCATTTTCAGAGGTCAAAAGCAGATAAGGTGGGACAAGGTGTGCATAAAGAAGGTGAAGTTTTACCTGCAGTTTCTGTAAGGTTGGAAATGGAGAATACTAGCAAAAAAGGTTCTAAACCTACTAAGTACTTTTCTGATGCTGAAGATATAAGTGATAGTAACCGGAGGTTAGAGCTTGCATGGCTGACAGTGACAAAAGCTCTTGAACCAGCTTTGCAATTGTTTAGGTGGGCACTGTCATCAGGTCTGTTTATGTTAGTAGGACTTCTCTTCTCTCTTGTGGGCAATCATTATTTCTTTACTTTGATCATTACTTGCTTTCGGGAAAAAATTGTATAGATTAAATATTTTATTACTCAACGAACACAATTTACTGTCTTCTAATTTTTTGTAGATGTACTTCTGTTTGTTTAAGACAGATGGTGGCCTATACTGTCTGCATTTTATTTTTGCTTTTGTCATGTTCTTTTTAAAGTTGCAGGCTGTGTCTAAGTTATAGTCTTGGAGGCTAATTGAGTTTTTGTGACTTACAGGGGATGAAATTCAGAATCAAATTCCACCTAGAAGTCGTTCTGTTTCAGAAATCATTGCAAGTATCCAGCGGAGTAAGATGGGAATCCAGGATTGGAGCTTGAGTGATCTTACAATTGGATTATGTCTTATATATCTCCGTCAAGCTTCAACAAATCCATTGGAGGACATTAAGGGTGTCCAGATCTCTTCAGATGCAATTGTAATCTGTTCACATCTTTTTTATTTTCTTATAAAATATATGGATTCATTGAAATTATGTTTTTCTTTCCCTTTAAGAAGTTTCAAACCCTTATAAGCCTGAATTGAGATCTTATATAGGTAATGATTTTGTGAATCAGGTCGAAGACCTCATCTACTACCTTGAACTAGCTGATGGTTCTTATAAGGACTCTGCAGCTATGCTTGCAAGAAGAACCATGCTTCGAGAGTGCAACATCTTGAAATTTGTTAAAGATTCCAGTGTTATGAGGCCTGGTTATTATATAGGGGTTGATACTAGAAAGAAACTTGTAATCTTTGGAATTCGAGGAACTCGCACAGTATATGATCTTATCACTGATATTATTACATCAAGCGACAGAGAAGAAGTGACATTTGAAGGATATTCTACGCACTTTGGCACTTCTGAATCTGCTCGCTGGTTTCTTGATCATGAGATCGGAATGATAAGAAGATGCTTGGAGAAATATCAGGTGGTATTTATGTTTACTAAAGTTCTTTTAGCTTATTCTTTACGTTCCTCCATCACCCTGTTACCATTGTCGTGGCTGTATCTAATTCATTAACAGGGTTTTAGGTTAAGGCTGGTGGGTCATTCTCTTGGTGGAGCTACAGCTTCGTTACTAGCTGTAATGCTTCGTAAAATGTCAAATAAGGAGCTTGGATTTAGCCCTGATATTGTGTCTGCCATTGGATTTGCAACACCACCTTGCGTTTCAAGGAAACTTGCTGAGAGTTGTGCTGACTATGTTACCACCGTAGTGATGCAGGTTTGCCACATGCAGTCCTCATATGTATTCTATAGCAAAAGCTACAATTTTTTTAAACATAATTTCCTAGAGTTTTATTTCGGTAGTTCTTGTTTCATTCTGTTGCTTCACTTATAGTCTCTGACTTTACTTTGGGCAGGATGATGTTATCCCTAGATTAAGTGTAGCTTCTCTAACCAGGCTAAGGATTGAAATTCTACAAACTGATTGGTAAGTTATTATGCTTTATTTGAAAGGCTAAAGGTAATTTTCTTCTATGGATGTGCTGAAAATAATATGGCTGTTGAAGGTGTGAGGAGACATGTTGAAAGCATTTTTAATGATTATGATATGATAAACCGGGTTTCAAAACCGTCCCTGTAAATCTCTGGAGTCGAAGGCTCTAAGTTAAGGGATCACCTAATCCTCTTGACAATAATTTTTATCTTTTATAATGTGATGACCACAATTCATATTCTGCATATGAATCTTGGTTACTGTCTTATCACAATATCTGTTATCTAAACCATACTATTAAGATCCCGTTTGATAACCATTTAGTTTTTTGTTCTTAAAAAGTAAGCTTATAGATACTACTTCCACCCCACCTATGGATTTCTTTGTTTTGTTATCCACTTTTTACATATGTTTTCAAAATTTAAGCCAAGTTTTGAAAACTAGAAAATGATTTTTTTTTTTTTTTTTTTGGCTAAGAATTCAAAATTTTTATTAAGAGAGATGAAAAATATTGTGAAAAAATGGTGAGAAAACCAGCCTAATCTTTAAAAACCAAAAACCAAAAATAAAAAACTAAATGGTTATCAAATGGAGCCCAAGTCGCGTTTATATGAAGTATTACAGCTGATAAATCAAGTTTAATTAGGAATTGGATTGAGAAATGTGATTTTAATTTCCTTATCATAGCTATCTTATGGAATTTTGTATTAATTGTATTGTGGACTGGTAGAATTCACTTAGATTCAAAAGATATTGAGATGCACTTAGGAAAGTTTGTGAGTCCCTTAAGTTTCCCTTCTTACTTAGTTACTTCTATTAAAGTATGGACTGAATAGACATTGCCATGAAAGTTTGGTGACCAAAACTTAAAATTTAACTATAATTGTTGAGTATATCAACCTTGTGCAGCTGTTAAACTTGTGATTTCGGAGTTTGCAAATTCAATTGCAGAACTTCAATTTACTATTCAGACATATGGTCAATGCTGCTTTTTAAAACTTCTATTCAATTGCTCTGAGGTTGACTAGATTTTGAAGATGTTACACATAGTCAGGCATCGATCTAATTTGGCACGAGCTATTTTGAGGGATAAAAAAAGAAAATATTATGGAATTTAATTAATGAGAGAAAATCAACATGCTGATTTCTTGTTATTGAACAAGCTAGTGGAGAAGGCAATCTCAAATTTCATGTTCCATCTGTTTCAGGATGAGTGTGATTGAAAAACAAGACTTGAAAGGTATCGTTGGTTTGGTCACAAATGCAAAGCAGGTGGTTACATCAGTTCAAGACGTTGCTCGAAAACTTGCAGATTTTGCAAAGTTCACAAGCAAGAAAAGATCCTCTGGTAAGCTAATCTATGGACCAAATATACGAAATATTGCTCCGCTATAATTTTACTGTCCTGCCAATCCTAATGTTCACAGGTTTACTTCACGTTTTCCTCAGGCATAAATTAATTGATTATTATTGTTTTAATGGCTTTCAGTTAGTTTTGGTCCTCGATTGGGCAAGATTAACCGAGCAGTCATGTGATGAAATTTTGTATCCATAATATTTTCTCCTTGACCGAATCTGCATCAAATTGACCAACTAACTTCTAGACTCTAAACAAGACTGTTCTTTTACCTTATGTTTTTATTTTTTATTTGCTATGTTTTTTCTCAAGCATATATTTACTAGCAAATGTAAATGTTCCTTGGTCAGAATTTATCTATTTCATGTCAATTTTACAGTTTTCGAGTCCAATAAAAGATTAGGGATAATTTTGGTAGATCTAGCCAATGTTTTCCAACAGGGAACTCTTCACAGAAGTATTTCGTTTTGATTTAATTTGAAGGAAATGTAGTAAAATCACACCTCCTCCACACTGCCCTATTACTAGTTTCTACCTTCGCCTCAGCCACTTTCTTTCTTCCCTTTTTAACAGATGATAGCAATAGAAAAGAGTCTCATGCGGCTTCTGGACCTCCTTCTTCACACCCTACCGTTGCGCTGCAGAGTGCTGCCGCTGCTGAAAATGAAGCCGCAAAATGTAAAATTCCCGAGGAGCTATTCGTACCGGGAACGGTTTATTATCTGAAGAGGCATATGGACAGTACCCCAGAATACTTCACTCTGTGGAAGCGGCATCCTGATGAACATTTCCAGCAAGTTGTGCTATCAAGCAACCTCATTTCGAATCATAAATGTGACAGCCACTACTATGCTTTGAGGGATGTACTTAAAAGCCTGCCATCATGCAACGATGAAGAAGGTATTTTCTCATAA

mRNA sequence

ATGGCCAGTCGAACTGCGAGGCGTCTTCTTCACAACTTCCATTATGCCAGAAGCTTTCTCAGGAAACAGGAGTGGCAGGCTAGCTCTGTAACTGTGAATAGTGTTCTTTTGCATTTTCAGAGGTCAAAAGCAGATAAGGTGGGACAAGGTGTGCATAAAGAAGGTGAAGTTTTACCTGCAGTTTCTGTAAGGTTGGAAATGGAGAATACTAGCAAAAAAGGTTCTAAACCTACTAAGTACTTTTCTGATGCTGAAGATATAAGTGATAGTAACCGGAGGTTAGAGCTTGCATGGCTGACAGTGACAAAAGCTCTTGAACCAGCTTTGCAATTGTTTAGGTGGGCACTGTCATCAGGGGATGAAATTCAGAATCAAATTCCACCTAGAAGTCGTTCTGTTTCAGAAATCATTGCAAGTATCCAGCGGAGTAAGATGGGAATCCAGGATTGGAGCTTGAGTGATCTTACAATTGGATTATGTCTTATATATCTCCGTCAAGCTTCAACAAATCCATTGGAGGACATTAAGGGTGTCCAGATCTCTTCAGATGCAATTGTCGAAGACCTCATCTACTACCTTGAACTAGCTGATGGTTCTTATAAGGACTCTGCAGCTATGCTTGCAAGAAGAACCATGCTTCGAGAGTGCAACATCTTGAAATTTGTTAAAGATTCCAGTGTTATGAGGCCTGGTTATTATATAGGGGTTGATACTAGAAAGAAACTTGTAATCTTTGGAATTCGAGGAACTCGCACAGTATATGATCTTATCACTGATATTATTACATCAAGCGACAGAGAAGAAGTGACATTTGAAGGATATTCTACGCACTTTGGCACTTCTGAATCTGCTCGCTGGTTTCTTGATCATGAGATCGGAATGATAAGAAGATGCTTGGAGAAATATCAGGGTTTTAGGTTAAGGCTGGTGGGTCATTCTCTTGGTGGAGCTACAGCTTCGTTACTAGCTGTAATGCTTCGTAAAATGTCAAATAAGGAGCTTGGATTTAGCCCTGATATTGTGTCTGCCATTGGATTTGCAACACCACCTTGCGTTTCAAGGAAACTTGCTGAGAGTTGTGCTGACTATGTTACCACCGTAGTGATGCAGGATGATGTTATCCCTAGATTAAGTGTAGCTTCTCTAACCAGGCTAAGGATTGAAATTCTACAAACTGATTGGATGAGTGTGATTGAAAAACAAGACTTGAAAGGTATCGTTGGTTTGGTCACAAATGCAAAGCAGGTGGTTACATCAGTTCAAGACGTTGCTCGAAAACTTGCAGATTTTGCAAAGTTCACAAGCAAGAAAAGATCCTCTGATGATAGCAATAGAAAAGAGTCTCATGCGGCTTCTGGACCTCCTTCTTCACACCCTACCGTTGCGCTGCAGAGTGCTGCCGCTGCTGAAAATGAAGCCGCAAAATGTAAAATTCCCGAGGAGCTATTCGTACCGGGAACGGTTTATTATCTGAAGAGGCATATGGACAGTACCCCAGAATACTTCACTCTGTGGAAGCGGCATCCTGATGAACATTTCCAGCAAGTTGTGCTATCAAGCAACCTCATTTCGAATCATAAATGTGACAGCCACTACTATGCTTTGAGGGATGTACTTAAAAGCCTGCCATCATGCAACGATGAAGAAGGTATTTTCTCATAA

Coding sequence (CDS)

ATGGCCAGTCGAACTGCGAGGCGTCTTCTTCACAACTTCCATTATGCCAGAAGCTTTCTCAGGAAACAGGAGTGGCAGGCTAGCTCTGTAACTGTGAATAGTGTTCTTTTGCATTTTCAGAGGTCAAAAGCAGATAAGGTGGGACAAGGTGTGCATAAAGAAGGTGAAGTTTTACCTGCAGTTTCTGTAAGGTTGGAAATGGAGAATACTAGCAAAAAAGGTTCTAAACCTACTAAGTACTTTTCTGATGCTGAAGATATAAGTGATAGTAACCGGAGGTTAGAGCTTGCATGGCTGACAGTGACAAAAGCTCTTGAACCAGCTTTGCAATTGTTTAGGTGGGCACTGTCATCAGGGGATGAAATTCAGAATCAAATTCCACCTAGAAGTCGTTCTGTTTCAGAAATCATTGCAAGTATCCAGCGGAGTAAGATGGGAATCCAGGATTGGAGCTTGAGTGATCTTACAATTGGATTATGTCTTATATATCTCCGTCAAGCTTCAACAAATCCATTGGAGGACATTAAGGGTGTCCAGATCTCTTCAGATGCAATTGTCGAAGACCTCATCTACTACCTTGAACTAGCTGATGGTTCTTATAAGGACTCTGCAGCTATGCTTGCAAGAAGAACCATGCTTCGAGAGTGCAACATCTTGAAATTTGTTAAAGATTCCAGTGTTATGAGGCCTGGTTATTATATAGGGGTTGATACTAGAAAGAAACTTGTAATCTTTGGAATTCGAGGAACTCGCACAGTATATGATCTTATCACTGATATTATTACATCAAGCGACAGAGAAGAAGTGACATTTGAAGGATATTCTACGCACTTTGGCACTTCTGAATCTGCTCGCTGGTTTCTTGATCATGAGATCGGAATGATAAGAAGATGCTTGGAGAAATATCAGGGTTTTAGGTTAAGGCTGGTGGGTCATTCTCTTGGTGGAGCTACAGCTTCGTTACTAGCTGTAATGCTTCGTAAAATGTCAAATAAGGAGCTTGGATTTAGCCCTGATATTGTGTCTGCCATTGGATTTGCAACACCACCTTGCGTTTCAAGGAAACTTGCTGAGAGTTGTGCTGACTATGTTACCACCGTAGTGATGCAGGATGATGTTATCCCTAGATTAAGTGTAGCTTCTCTAACCAGGCTAAGGATTGAAATTCTACAAACTGATTGGATGAGTGTGATTGAAAAACAAGACTTGAAAGGTATCGTTGGTTTGGTCACAAATGCAAAGCAGGTGGTTACATCAGTTCAAGACGTTGCTCGAAAACTTGCAGATTTTGCAAAGTTCACAAGCAAGAAAAGATCCTCTGATGATAGCAATAGAAAAGAGTCTCATGCGGCTTCTGGACCTCCTTCTTCACACCCTACCGTTGCGCTGCAGAGTGCTGCCGCTGCTGAAAATGAAGCCGCAAAATGTAAAATTCCCGAGGAGCTATTCGTACCGGGAACGGTTTATTATCTGAAGAGGCATATGGACAGTACCCCAGAATACTTCACTCTGTGGAAGCGGCATCCTGATGAACATTTCCAGCAAGTTGTGCTATCAAGCAACCTCATTTCGAATCATAAATGTGACAGCCACTACTATGCTTTGAGGGATGTACTTAAAAGCCTGCCATCATGCAACGATGAAGAAGGTATTTTCTCATAA

Protein sequence

MASRTARRLLHNFHYARSFLRKQEWQASSVTVNSVLLHFQRSKADKVGQGVHKEGEVLPAVSVRLEMENTSKKGSKPTKYFSDAEDISDSNRRLELAWLTVTKALEPALQLFRWALSSGDEIQNQIPPRSRSVSEIIASIQRSKMGIQDWSLSDLTIGLCLIYLRQASTNPLEDIKGVQISSDAIVEDLIYYLELADGSYKDSAAMLARRTMLRECNILKFVKDSSVMRPGYYIGVDTRKKLVIFGIRGTRTVYDLITDIITSSDREEVTFEGYSTHFGTSESARWFLDHEIGMIRRCLEKYQGFRLRLVGHSLGGATASLLAVMLRKMSNKELGFSPDIVSAIGFATPPCVSRKLAESCADYVTTVVMQDDVIPRLSVASLTRLRIEILQTDWMSVIEKQDLKGIVGLVTNAKQVVTSVQDVARKLADFAKFTSKKRSSDDSNRKESHAASGPPSSHPTVALQSAAAAENEAAKCKIPEELFVPGTVYYLKRHMDSTPEYFTLWKRHPDEHFQQVVLSSNLISNHKCDSHYYALRDVLKSLPSCNDEEGIFS

Homology

[Close]

BLAST of Lag0001992 vs. NCBI nr
Match: XP_038897252.1 (uncharacterized protein LOC120085373 isoform X1 [Benincasa hispida])

HSP 1 Score: 890.6 bits (2300), Expect = 7.0e-255
Identity = 461/548 (84.12%), Postives = 498/548 (90.88%), Query Frame = 0


Query: 1   MASRTARRLLHNFHYARSFLRKQEWQASSVTVNSVLLHFQRSKADKVGQGVHKEGEVLPA 60
           MA+ TARRLLHNFHYAR+FLRKQ+WQA SVTVN+VL+HFQR+K DKV +GVHKEGEVL  
Sbjct: 1   MANGTARRLLHNFHYARNFLRKQQWQARSVTVNNVLMHFQRTKVDKVEKGVHKEGEVLQE 60

Query: 61  VSVRLEMENTSKKGSKPTKYFSDAEDISDSNRRLELAWLTVTKALEPALQLFRWALSSGD 120
           VS R EM   SKKGSK TK  S+AED +DSNR LELAW   TKA+EPALQL+RWALSSGD
Sbjct: 61  VSGRSEMGKNSKKGSKRTKSVSEAEDTTDSNRMLELAWW--TKAIEPALQLYRWALSSGD 120

Query: 121 EIQNQIPPRSRSVSEIIASIQRSKMGIQDWSLSDLTIGLCLIYLRQASTNPLEDIKGVQI 180
                IPPR+RSVSEIIASIQRSK GIQDWSLSDLTIGLCLIYLRQASTNPLED+KGVQI
Sbjct: 121 ----GIPPRTRSVSEIIASIQRSKTGIQDWSLSDLTIGLCLIYLRQASTNPLEDLKGVQI 180

Query: 181 SSDAIVEDLIYYLELADGSYKDSAAMLARRTMLRECNILKFVKDSSVMRPGYYIGVDTRK 240
           SSDA+VEDLIY++ELA GSYK+SAAMLA+ TMLRECNILKFVK+SSVMRPGYYIGVDTRK
Sbjct: 181 SSDAMVEDLIYFVELAKGSYKNSAAMLAKTTMLRECNILKFVKNSSVMRPGYYIGVDTRK 240

Query: 241 KLVIFGIRGTRTVYDLITDIITSSDREEVTFEGYSTHFGTSESARWFLDHEIGMIRRCLE 300
           KLVIFGIRGT TVYDLITDIIT+SD  +VTFEGYS HFGTSESARWFL HEIGMIRRCLE
Sbjct: 241 KLVIFGIRGTYTVYDLITDIITTSD-GDVTFEGYSMHFGTSESARWFLQHEIGMIRRCLE 300

Query: 301 KYQGFRLRLVGHSLGGATASLLAVMLRKMSNKELGFSPDIVSAIGFATPPCVSRKLAESC 360
           KYQGFRL+LVGHSLGGA ASLLAVMLRKMSNKELGFSPDIVSAIG+ATPPCVSRKLAESC
Sbjct: 301 KYQGFRLKLVGHSLGGAIASLLAVMLRKMSNKELGFSPDIVSAIGYATPPCVSRKLAESC 360

Query: 361 ADYVTTVVMQDDVIPRLSVASLTRLRIEILQTDWMSVIEKQDLKGIVGLVTNAKQVVTSV 420
           ADYVTTVVMQ DVIPRLSVASLTRLRIEILQTDWMS+I+K+D K ++GLVTNAKQVVTSV
Sbjct: 361 ADYVTTVVMQGDVIPRLSVASLTRLRIEILQTDWMSLIDKEDWKSVIGLVTNAKQVVTSV 420

Query: 421 QDVARKLADFAKFTSKKRSSDDSNRKESHAASGPPSSHPTVALQSAAAAENEAAKCKIPE 480
           QDVARKLADFAKF SKKRSSDDSNRK+S  ASG P  HPT ALQSA A +NEAA+CKIP+
Sbjct: 421 QDVARKLADFAKFPSKKRSSDDSNRKKSDVASGSP-PHPTAALQSATATQNEAARCKIPD 480

Query: 481 ELFVPGTVYYLKRHMDSTPEYFTLWKRHPDEHFQQVVLSSNLISNHKCDSHYYALRDVLK 540
           ELFVPGTVYYLKRH DSTPEYFTLWKR+PDEHFQQ+VLSS LIS+H CDSHYYALRDVLK
Sbjct: 481 ELFVPGTVYYLKRHTDSTPEYFTLWKRYPDEHFQQIVLSSILISDHTCDSHYYALRDVLK 540

Query: 541 SLPSCNDE 549
            LPSC+ +
Sbjct: 541 GLPSCSQQ 540

[Close]

BLAST of Lag0001992 vs. NCBI nr
Match: XP_023513693.1 (sn1-specific diacylglycerol lipase beta [Cucurbita pepo subsp. pepo])

HSP 1 Score: 886.3 bits (2289), Expect = 1.3e-253
Identity = 465/555 (83.78%), Postives = 495/555 (89.19%), Query Frame = 0


Query: 1   MASRTARRLLHNFHYARSFLRKQEWQASSVTVNSVLLHFQRSKADKVGQGVHKEGEVLPA 60
           M++ TARRLLHN + ARS LRKQ+WQASSVTVNSVL+H QRSKAD V   V KE EVLP 
Sbjct: 1   MSNGTARRLLHNLYSARSSLRKQKWQASSVTVNSVLMHLQRSKADMVENNVRKENEVLPE 60

Query: 61  VSVRLEMENTSKKGSKPTKYFSDAEDISDSNRRLELAWLTVTKALEPALQLFRWALSSGD 120
           VSVR EME  SK+GSK  KYFSDAEDISDSNRRLELAWL  TKAL+ A QL+RWALSSGD
Sbjct: 61  VSVRSEMEKNSKEGSKTPKYFSDAEDISDSNRRLELAWL--TKALKSASQLYRWALSSGD 120

Query: 121 EIQNQIPPRSRSVSEIIASIQRSKMGIQDWSLSDLTIGLCLIYLRQASTNPLEDIKGVQI 180
            IQNQI PRSRSVSEIIASIQRSKMGIQ+WSLSDLTIGL LIYLRQASTNPL DIKGVQI
Sbjct: 121 GIQNQIQPRSRSVSEIIASIQRSKMGIQEWSLSDLTIGLFLIYLRQASTNPLADIKGVQI 180

Query: 181 SSDAIVEDLIYYLELADGSYKDSAAMLARRTMLRECNILKFVKDSSVMRPGYYIGVDTRK 240
           +S+AIVEDLIYYLELA G YKDSAAMLAR TMLRE NILKF+KDSSVMRPGYYIGVDTRK
Sbjct: 181 TSNAIVEDLIYYLELAKGCYKDSAAMLARNTMLREPNILKFIKDSSVMRPGYYIGVDTRK 240

Query: 241 KLVIFGIRGTRTVYDLITDIITSSDREEVTFEGYSTHFGTSESARWFLDHEIGMIRRCLE 300
           KLVIFGIRGT +VYDLITDI TSSD EEVT EGYSTHFGTSESARWFLDHEI MIRRCL+
Sbjct: 241 KLVIFGIRGTHSVYDLITDITTSSD-EEVTLEGYSTHFGTSESARWFLDHEIEMIRRCLK 300

Query: 301 KYQGFRLRLVGHSLGGATASLLAVMLRKMSNKELGFSPDIVSAIGFATPPCVSRKLAESC 360
           KYQGFRLRLVGHSLGGA ASLLAVMLRK SNKELGFSPDIVSAIGFATPPCVSRKLAESC
Sbjct: 301 KYQGFRLRLVGHSLGGAIASLLAVMLRKKSNKELGFSPDIVSAIGFATPPCVSRKLAESC 360

Query: 361 ADYVTTVVMQDDVIPRLSVASLTRLRIEILQTDWMSVIEKQDLKGIVGLVTNAKQVVTSV 420
           ADYVTTVVMQDD+IPRLS+ASLT+LR+EILQTDW SVIE+QD K I GL+TNAKQVVTSV
Sbjct: 361 ADYVTTVVMQDDIIPRLSLASLTKLRVEILQTDWTSVIEEQDWKNIYGLLTNAKQVVTSV 420

Query: 421 QDVARKLADFAKFTSKKRSSDDSNRKESHAASGPPSSHPTVALQSAAAAENEAAKCKIPE 480
           QDVARKL D+AKFTSKK  SDDS  K SH ASGPP SHPT  +Q A  A+NEAA+CKIPE
Sbjct: 421 QDVARKLGDYAKFTSKKGPSDDSYGKGSHVASGPPPSHPTAVVQRADTAQNEAARCKIPE 480

Query: 481 ELFVPGTVYYLKRH--MDSTPEYFTLWKRHPDEHFQQVVLSSNLISNHKCDSHYYALRDV 540
           ELFVPGTVYYLK+H  ++   EYFTLWKRHPDEHFQQ+VLSSNLIS+HKCDSHY+ALRDV
Sbjct: 481 ELFVPGTVYYLKKHVNLEEAYEYFTLWKRHPDEHFQQIVLSSNLISDHKCDSHYHALRDV 540

Query: 541 LKSLPSCNDEEGIFS 554
           LK LPSCND EGIFS
Sbjct: 541 LKGLPSCND-EGIFS 551

[Close]

BLAST of Lag0001992 vs. NCBI nr
Match: XP_022959891.1 (sn1-specific diacylglycerol lipase beta [Cucurbita moschata])

HSP 1 Score: 882.9 bits (2280), Expect = 1.5e-252
Identity = 464/555 (83.60%), Postives = 494/555 (89.01%), Query Frame = 0


Query: 1   MASRTARRLLHNFHYARSFLRKQEWQASSVTVNSVLLHFQRSKADKVGQGVHKEGEVLPA 60
           M++ TARRLLHN + ARS LRKQ+WQASSVTVNSVL+H QRSKAD V   V KE EVLP 
Sbjct: 1   MSNGTARRLLHNLYSARSSLRKQKWQASSVTVNSVLMHLQRSKADMVENNVRKENEVLPE 60

Query: 61  VSVRLEMENTSKKGSKPTKYFSDAEDISDSNRRLELAWLTVTKALEPALQLFRWALSSGD 120
           VSVR EME  SK+GSK  KYFSDAEDISDSNRRLELAWL  TKAL+ A QL+RWALSSGD
Sbjct: 61  VSVRSEMEKNSKEGSKTPKYFSDAEDISDSNRRLELAWL--TKALKSASQLYRWALSSGD 120

Query: 121 EIQNQIPPRSRSVSEIIASIQRSKMGIQDWSLSDLTIGLCLIYLRQASTNPLEDIKGVQI 180
            IQNQI PRSRSVSEIIASIQRSKMGIQ+WSLSDLTIGL LIYLRQASTNPL DIKGVQI
Sbjct: 121 GIQNQIQPRSRSVSEIIASIQRSKMGIQEWSLSDLTIGLFLIYLRQASTNPLADIKGVQI 180

Query: 181 SSDAIVEDLIYYLELADGSYKDSAAMLARRTMLRECNILKFVKDSSVMRPGYYIGVDTRK 240
           +S+AIVEDLIYYLELA G YKDSAAMLAR TMLRE NILKF+KDSSVMRPGYYIGVDTRK
Sbjct: 181 TSNAIVEDLIYYLELAKGCYKDSAAMLARNTMLREPNILKFIKDSSVMRPGYYIGVDTRK 240

Query: 241 KLVIFGIRGTRTVYDLITDIITSSDREEVTFEGYSTHFGTSESARWFLDHEIGMIRRCLE 300
           KLVIFGIRGT +VYDLITDI TSSD EEVT EGYSTHFGTSESARWFLDHEI MIRRCL+
Sbjct: 241 KLVIFGIRGTHSVYDLITDITTSSD-EEVTLEGYSTHFGTSESARWFLDHEIEMIRRCLK 300

Query: 301 KYQGFRLRLVGHSLGGATASLLAVMLRKMSNKELGFSPDIVSAIGFATPPCVSRKLAESC 360
           KYQGFRLRLVGHSLGGA ASLLAVMLRK SNKELGFSPDIVSAIGFATPPCVSRKLAESC
Sbjct: 301 KYQGFRLRLVGHSLGGAIASLLAVMLRKKSNKELGFSPDIVSAIGFATPPCVSRKLAESC 360

Query: 361 ADYVTTVVMQDDVIPRLSVASLTRLRIEILQTDWMSVIEKQDLKGIVGLVTNAKQVVTSV 420
           ADYVTTVVMQDD+IPRLS+ASLT+LR+EILQTDW SVIE+QD K I GL+TNAKQVVTSV
Sbjct: 361 ADYVTTVVMQDDIIPRLSLASLTKLRVEILQTDWTSVIEEQDWKNIYGLLTNAKQVVTSV 420

Query: 421 QDVARKLADFAKFTSKKRSSDDSNRKESHAASGPPSSHPTVALQSAAAAENEAAKCKIPE 480
           QDVARKL D+AKFTSKK  SDDS  K SH ASGPP S PT  +Q A  A+NEAA+CKIPE
Sbjct: 421 QDVARKLGDYAKFTSKKGPSDDSYGKGSHVASGPPPSQPTALVQRADTAQNEAARCKIPE 480

Query: 481 ELFVPGTVYYLKRH--MDSTPEYFTLWKRHPDEHFQQVVLSSNLISNHKCDSHYYALRDV 540
           ELFVPGTVYYLK+H  ++   EYFTLWKRHPDEHFQQ+VLSSNLIS+HKCDSHY+ALRDV
Sbjct: 481 ELFVPGTVYYLKKHVNLEEAYEYFTLWKRHPDEHFQQIVLSSNLISDHKCDSHYHALRDV 540

Query: 541 LKSLPSCNDEEGIFS 554
           LK LPSCND EGIFS
Sbjct: 541 LKGLPSCND-EGIFS 551

[Close]

BLAST of Lag0001992 vs. NCBI nr
Match: XP_023004816.1 (sn1-specific diacylglycerol lipase beta [Cucurbita maxima])

HSP 1 Score: 880.6 bits (2274), Expect = 7.3e-252
Identity = 463/555 (83.42%), Postives = 493/555 (88.83%), Query Frame = 0


Query: 1   MASRTARRLLHNFHYARSFLRKQEWQASSVTVNSVLLHFQRSKADKVGQGVHKEGEVLPA 60
           M++ TARRLL N + ARS+LRKQ+WQASSVTVNSVL+H QRSKAD V   V KE EVLP 
Sbjct: 1   MSNGTARRLLRNLYSARSYLRKQKWQASSVTVNSVLMHLQRSKADMVENNVRKENEVLPE 60

Query: 61  VSVRLEMENTSKKGSKPTKYFSDAEDISDSNRRLELAWLTVTKALEPALQLFRWALSSGD 120
           VSVR EME   KKGSK  KYFSDAEDISDSNRRLELAWL  TKAL+ A QL+RWALSSGD
Sbjct: 61  VSVRSEMEKNIKKGSKTPKYFSDAEDISDSNRRLELAWL--TKALKSASQLYRWALSSGD 120

Query: 121 EIQNQIPPRSRSVSEIIASIQRSKMGIQDWSLSDLTIGLCLIYLRQASTNPLEDIKGVQI 180
            IQNQI PRSRSVSEIIASIQRSKMGIQ+WSLSDLTIGL LIYLRQASTNPL DIKGVQI
Sbjct: 121 GIQNQIQPRSRSVSEIIASIQRSKMGIQEWSLSDLTIGLFLIYLRQASTNPLADIKGVQI 180

Query: 181 SSDAIVEDLIYYLELADGSYKDSAAMLARRTMLRECNILKFVKDSSVMRPGYYIGVDTRK 240
           +S+AIVEDLIYYLELA G YKDSAAMLAR TMLRE NILKF+KDSSVMRPGYYIGVDTRK
Sbjct: 181 TSNAIVEDLIYYLELAKGCYKDSAAMLARNTMLREPNILKFIKDSSVMRPGYYIGVDTRK 240

Query: 241 KLVIFGIRGTRTVYDLITDIITSSDREEVTFEGYSTHFGTSESARWFLDHEIGMIRRCLE 300
           KLVIFGIRGT +VYDLITDI TSSD EEVTFEGYSTHFGTSESARWFLDHEI MIR+CL+
Sbjct: 241 KLVIFGIRGTHSVYDLITDITTSSD-EEVTFEGYSTHFGTSESARWFLDHEIEMIRQCLK 300

Query: 301 KYQGFRLRLVGHSLGGATASLLAVMLRKMSNKELGFSPDIVSAIGFATPPCVSRKLAESC 360
           KYQGFRLRLVGHSLGGA ASLLAVMLRK SNKELGF PDIVSAIGFATPPCVSRKLAESC
Sbjct: 301 KYQGFRLRLVGHSLGGAIASLLAVMLRKKSNKELGFRPDIVSAIGFATPPCVSRKLAESC 360

Query: 361 ADYVTTVVMQDDVIPRLSVASLTRLRIEILQTDWMSVIEKQDLKGIVGLVTNAKQVVTSV 420
           ADYVTTVVMQDD+IPRLS+ASLT+LRIEILQTDW SVIE+QD K I GL+TNAKQVVTSV
Sbjct: 361 ADYVTTVVMQDDIIPRLSLASLTKLRIEILQTDWTSVIEEQDWKNIYGLLTNAKQVVTSV 420

Query: 421 QDVARKLADFAKFTSKKRSSDDSNRKESHAASGPPSSHPTVALQSAAAAENEAAKCKIPE 480
           QDVARKL D+AKFTSKK  SDDS  K SH ASGPP S PT  +Q A  A+NEAA+CKIPE
Sbjct: 421 QDVARKLGDYAKFTSKKGPSDDSYGKGSHVASGPPPSQPTAVVQRADTAQNEAARCKIPE 480

Query: 481 ELFVPGTVYYLKRH--MDSTPEYFTLWKRHPDEHFQQVVLSSNLISNHKCDSHYYALRDV 540
           ELFVPGTVYYLK+H  ++   EYFTLWKRHPDEHFQQ+VLSSNLIS+HKCDSHY+ALRDV
Sbjct: 481 ELFVPGTVYYLKKHVNLEEAYEYFTLWKRHPDEHFQQIVLSSNLISDHKCDSHYHALRDV 540

Query: 541 LKSLPSCNDEEGIFS 554
           LK LPSCND EGIFS
Sbjct: 541 LKGLPSCND-EGIFS 551

[Close]

BLAST of Lag0001992 vs. NCBI nr
Match: XP_022156622.1 (uncharacterized protein LOC111023479 [Momordica charantia])

HSP 1 Score: 866.7 bits (2238), Expect = 1.1e-247
Identity = 460/554 (83.03%), Postives = 491/554 (88.63%), Query Frame = 0


Query: 1   MASRTARRLLHNFHYARSFLRKQEWQASSVTVNSVLLHFQRSKADKVGQGVHKEGEVLPA 60
           MAS   RRLLHNFH+ARSFLRKQE QASSVTVNSVL+HF RSKADKV  GVHKEGEVLP 
Sbjct: 1   MASGAVRRLLHNFHHARSFLRKQELQASSVTVNSVLMHFHRSKADKVEIGVHKEGEVLPE 60

Query: 61  VSVRLEMENTSKKGSKPTKYFSDAEDISDSNRRLELAWLTVTKALEPALQLFRWALS-SG 120
           VSVR EME  SKKGS+PTKYFSD E+ISDS RR ELAWL  TKALEPALQL+RWALS SG
Sbjct: 61  VSVRSEMEKNSKKGSEPTKYFSDDENISDSKRRSELAWL--TKALEPALQLYRWALSASG 120

Query: 121 DEIQNQIPPRSRSVSEIIASIQRSKMGIQDWSLSDLTIGLCLIYLRQASTNPLEDIKGVQ 180
           D I++Q PPRSRSVSEIIASIQ SKMGIQDWSLSDLTIGL LIYLRQASTNP EDI GVQ
Sbjct: 121 DGIRDQTPPRSRSVSEIIASIQWSKMGIQDWSLSDLTIGLYLIYLRQASTNPFEDISGVQ 180

Query: 181 ISSDAIVEDLIYYLELADGSYKDSAAMLARRTMLRECNILKFVKDSSVMRPGYYIGVDTR 240
           ISSDAIV+DLIYYLELA GSYKDSAA LAR +MLRECNILKFV DSSVMRPGYYIGVDTR
Sbjct: 181 ISSDAIVQDLIYYLELAKGSYKDSAASLARNSMLRECNILKFVGDSSVMRPGYYIGVDTR 240

Query: 241 KKLVIFGIRGTRTVYDLITDIITSSDREEVTFEGYSTHFGTSESARWFLDHEIGMIRRCL 300
           KKLVIFGIRGT T+ DLITDI+TSSD  EVTFEGY THFGT+ESARWFLDHE+GMIRRCL
Sbjct: 241 KKLVIFGIRGTHTLSDLITDIVTSSD-GEVTFEGYVTHFGTAESARWFLDHEMGMIRRCL 300

Query: 301 EKYQGFRLRLVGHSLGGATASLLAVMLRKMSNKELGFSPDIVSAIGFATPPCVSRKLAES 360
           EKYQGFRLRLVGHSLGGA ASLLAVMLRK SNKELGFSPDIVSAIG+ATPPCVSRKLAES
Sbjct: 301 EKYQGFRLRLVGHSLGGAIASLLAVMLRKKSNKELGFSPDIVSAIGYATPPCVSRKLAES 360

Query: 361 CADYVTTVVMQDDVIPRLSVASLTRLRIEILQTDWMSVIEKQDLKGIVGLVTNAKQVVTS 420
           CADYVTTVVMQDD+IPRLS+ASLTRLR EILQTDW SVIEKQD K  +GLVTNA+QVV+S
Sbjct: 361 CADYVTTVVMQDDIIPRLSIASLTRLRNEILQTDWTSVIEKQDWKSFIGLVTNARQVVSS 420

Query: 421 VQDVARKLADFAKFTSKKRSSDDSNRKESHAASGPPSSHPTVALQSAAAAENEAAKCKIP 480
           VQDVARKLAD+AKFTSKK SSD S RKESH AS PP  HPT A ++ AA     A+ K P
Sbjct: 421 VQDVARKLADYAKFTSKKISSDGSIRKESHVASAPP--HPTAAARNKAA----TARYKTP 480

Query: 481 EELFVPGTVYYLKRHMDSTPEYFTLWKRHPDEHFQQVVLSSNLISNHKCDSHYYALRDVL 540
           EELFVPGTVYYLKR+ + TPEYFTLWKRHP EHFQ++VLSSNLIS+H+CDSHYYALRDVL
Sbjct: 481 EELFVPGTVYYLKRNTNGTPEYFTLWKRHPGEHFQRIVLSSNLISDHRCDSHYYALRDVL 540

Query: 541 KSLPSCNDEEGIFS 554
           K LP+ +D E IFS
Sbjct: 541 KGLPARSD-ESIFS 544

[Close]

BLAST of Lag0001992 vs. ExPASy Swiss-Prot
Match: P0C1S9 (Diacylglycerol lipase-beta OS=Rattus norvegicus OX=10116 GN=Daglb PE=1 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 2.3e-14
Identity = 56/165 (33.94%), Postives = 93/165 (56.36%), Query Frame = 0


Query: 232 YYIGVDTRKKLVIFGIRGTRTVYDLITDIITSSDREEVTFE--GYSTHFGTSESARWFLD 291
           + + +D RK+ V+  +RGT ++ D++TD+   S+  E+  E      H G +++AR+   
Sbjct: 358 FIVVLDHRKESVVVAVRGTMSLQDVLTDLSAESENLELDIELQDCVAHKGIAQAARYIYR 417

Query: 292 HEI--GMIRRCLEKYQGFRLRLVGHSLGGATASLLAVMLRKMSNKELGFSPDIVSAIGFA 351
             +  G++ +       +RL +VGHSLG   A+LLA+MLR       G  P  V A  F+
Sbjct: 418 RLVNDGILSQAFSVAPEYRLVVVGHSLGAGAAALLAIMLR-------GAYPQ-VRAYAFS 477

Query: 352 TP-PCVSRKLAESCADYVTTVVMQDDVIPRLSVASLTRLRIEILQ 392
            P   +S+ L E   D+V ++++  DVIPRLSVA++  L+  IL+
Sbjct: 478 PPRGLLSKSLFEYSKDFVVSLILGMDVIPRLSVANMEDLKRRILR 514

[Close]

BLAST of Lag0001992 vs. ExPASy Swiss-Prot
Match: Q91WC9 (Diacylglycerol lipase-beta OS=Mus musculus OX=10090 GN=Daglb PE=1 SV=2)

HSP 1 Score: 79.7 bits (195), Expect = 1.1e-13
Identity = 58/192 (30.21%), Postives = 101/192 (52.60%), Query Frame = 0


Query: 205 AMLARRTMLRECNILKFVKDSSVMRPGYYIGVDTRKKLVIFGIRGTRTVYDLITDIITSS 264
           A + + T L+  + +       V    + + +D RK+ V+  +RGT ++ D++TD+   S
Sbjct: 331 ASILKTTGLQYRDFIHISFHDKVYELPFIVVLDHRKESVVVAVRGTMSLQDVLTDLSAES 390

Query: 265 DREE--VTFEGYSTHFGTSESARWFLDHEI--GMIRRCLEKYQGFRLRLVGHSLGGATAS 324
           +  E  +  +    H G +++AR+     +  G++ +       ++L LVGHSLG   A+
Sbjct: 391 ETLELGIELQDCVAHKGIAQAARYIHRRLVNDGILSQAFSVAPEYQLVLVGHSLGAGAAA 450

Query: 325 LLAVMLRKMSNKELGFSPDIVSAIGFATP-PCVSRKLAESCADYVTTVVMQDDVIPRLSV 384
           LLA+MLR       G  P  V A  F+ P   +S+ L E   D+V ++++  DVIPRLSV
Sbjct: 451 LLAIMLR-------GAYPQ-VRAYAFSPPRGLLSKSLYEYSKDFVVSLILGMDVIPRLSV 510

Query: 385 ASLTRLRIEILQ 392
            ++  L+  IL+
Sbjct: 511 TNMEDLKRRILR 514

[Close]

BLAST of Lag0001992 vs. ExPASy Swiss-Prot
Match: Q8NCG7 (Diacylglycerol lipase-beta OS=Homo sapiens OX=9606 GN=DAGLB PE=1 SV=2)

HSP 1 Score: 79.3 bits (194), Expect = 1.5e-13
Identity = 55/165 (33.33%), Postives = 89/165 (53.94%), Query Frame = 0


Query: 232 YYIGVDTRKKLVIFGIRGTRTVYDLITDIITSSDREEVTFEGYS--THFGTSESARWFLD 291
           + + +D RK+ V+  +RGT ++ D++TD+   S+  +V  E      H G S++AR+   
Sbjct: 358 FLVALDHRKESVVVAVRGTMSLQDVLTDLSAESEVLDVECEVQDRLAHKGISQAARYVYQ 417

Query: 292 HEI--GMIRRCLEKYQGFRLRLVGHSLGGATASLLAVMLRKMSNKELGFSPDIVSAIGFA 351
             I  G++ +       +RL +VGHSLGG  A+LLA MLR    +        V    F+
Sbjct: 418 RLINDGILSQAFSIAPEYRLVIVGHSLGGGAAALLATMLRAAYPQ--------VRCYAFS 477

Query: 352 TPPCV-SRKLAESCADYVTTVVMQDDVIPRLSVASLTRLRIEILQ 392
            P  + S+ L E    ++ ++V+  DVIPRLSV +L  L+  IL+
Sbjct: 478 PPRGLWSKALQEYSQSFIVSLVLGKDVIPRLSVTNLEDLKRRILR 514

[Close]

BLAST of Lag0001992 vs. ExPASy Swiss-Prot
Match: Q9Y4D2 (Diacylglycerol lipase-alpha OS=Homo sapiens OX=9606 GN=DAGLA PE=1 SV=3)

HSP 1 Score: 61.2 bits (147), Expect = 4.2e-08
Identity = 54/180 (30.00%), Postives = 90/180 (50.00%), Query Frame = 0


Query: 226 SVMRPGYYIGVDTRKKLVIFGIRGTRTVYDLITDIITSSDREEVTFEGYS----THFGTS 285
           +V    +Y+ VD  KK V+  IRGT +  D +TD+  + D E +  EG+      H G  
Sbjct: 376 AVYETPFYVAVDHDKKKVVISIRGTLSPKDALTDL--TGDAERLPVEGHHGTWLGHKGMV 435

Query: 286 ESARWF---LDHEIGMIRRCLEKYQG-----FRLRLVGHSLGGATASLLAVMLRKMSNKE 345
            SA +    L+ E+ ++ +   +  G     + L +VGHSLG  TA++L+ +LR      
Sbjct: 436 LSAEYIKKKLEQEM-VLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLR------ 495

Query: 346 LGFSPDIVSAIGFA-TPP--CVSRKLAESCADYVTTVVMQDDVIPRLSVASLTRLRIEIL 391
               P   +   FA +PP   +S    E   ++VT VV+  D++PR+ ++ L   R ++L
Sbjct: 496 ----PQYPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQLL 542

[Close]

BLAST of Lag0001992 vs. ExPASy Swiss-Prot
Match: Q6WQJ1 (Diacylglycerol lipase-alpha OS=Mus musculus OX=10090 GN=Dagla PE=1 SV=2)

HSP 1 Score: 61.2 bits (147), Expect = 4.2e-08
Identity = 54/180 (30.00%), Postives = 90/180 (50.00%), Query Frame = 0


Query: 226 SVMRPGYYIGVDTRKKLVIFGIRGTRTVYDLITDIITSSDREEVTFEGYS----THFGTS 285
           +V    +Y+ VD  KK V+  IRGT +  D +TD+  + D E +  EG+      H G  
Sbjct: 376 AVYETPFYVAVDHDKKKVVISIRGTLSPKDALTDL--TGDAERLPVEGHRGTWLGHKGMV 435

Query: 286 ESARWF---LDHEIGMIRRCLEKYQG-----FRLRLVGHSLGGATASLLAVMLRKMSNKE 345
            SA +    L+ E+ ++ +   +  G     + L +VGHSLG  TA++L+ +LR      
Sbjct: 436 LSAEYIKKKLEQEM-VLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLR------ 495

Query: 346 LGFSPDIVSAIGFA-TPP--CVSRKLAESCADYVTTVVMQDDVIPRLSVASLTRLRIEIL 391
               P   +   FA +PP   +S    E   ++VT VV+  D++PR+ ++ L   R ++L
Sbjct: 496 ----PQYPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQLL 542

[Close]

BLAST of Lag0001992 vs. ExPASy TrEMBL
Match: A0A6J1H649 (sn1-specific diacylglycerol lipase beta OS=Cucurbita moschata OX=3662 GN=LOC111460812 PE=4 SV=1)

HSP 1 Score: 882.9 bits (2280), Expect = 7.1e-253
Identity = 464/555 (83.60%), Postives = 494/555 (89.01%), Query Frame = 0


Query: 1   MASRTARRLLHNFHYARSFLRKQEWQASSVTVNSVLLHFQRSKADKVGQGVHKEGEVLPA 60
           M++ TARRLLHN + ARS LRKQ+WQASSVTVNSVL+H QRSKAD V   V KE EVLP 
Sbjct: 1   MSNGTARRLLHNLYSARSSLRKQKWQASSVTVNSVLMHLQRSKADMVENNVRKENEVLPE 60

Query: 61  VSVRLEMENTSKKGSKPTKYFSDAEDISDSNRRLELAWLTVTKALEPALQLFRWALSSGD 120
           VSVR EME  SK+GSK  KYFSDAEDISDSNRRLELAWL  TKAL+ A QL+RWALSSGD
Sbjct: 61  VSVRSEMEKNSKEGSKTPKYFSDAEDISDSNRRLELAWL--TKALKSASQLYRWALSSGD 120

Query: 121 EIQNQIPPRSRSVSEIIASIQRSKMGIQDWSLSDLTIGLCLIYLRQASTNPLEDIKGVQI 180
            IQNQI PRSRSVSEIIASIQRSKMGIQ+WSLSDLTIGL LIYLRQASTNPL DIKGVQI
Sbjct: 121 GIQNQIQPRSRSVSEIIASIQRSKMGIQEWSLSDLTIGLFLIYLRQASTNPLADIKGVQI 180

Query: 181 SSDAIVEDLIYYLELADGSYKDSAAMLARRTMLRECNILKFVKDSSVMRPGYYIGVDTRK 240
           +S+AIVEDLIYYLELA G YKDSAAMLAR TMLRE NILKF+KDSSVMRPGYYIGVDTRK
Sbjct: 181 TSNAIVEDLIYYLELAKGCYKDSAAMLARNTMLREPNILKFIKDSSVMRPGYYIGVDTRK 240

Query: 241 KLVIFGIRGTRTVYDLITDIITSSDREEVTFEGYSTHFGTSESARWFLDHEIGMIRRCLE 300
           KLVIFGIRGT +VYDLITDI TSSD EEVT EGYSTHFGTSESARWFLDHEI MIRRCL+
Sbjct: 241 KLVIFGIRGTHSVYDLITDITTSSD-EEVTLEGYSTHFGTSESARWFLDHEIEMIRRCLK 300

Query: 301 KYQGFRLRLVGHSLGGATASLLAVMLRKMSNKELGFSPDIVSAIGFATPPCVSRKLAESC 360
           KYQGFRLRLVGHSLGGA ASLLAVMLRK SNKELGFSPDIVSAIGFATPPCVSRKLAESC
Sbjct: 301 KYQGFRLRLVGHSLGGAIASLLAVMLRKKSNKELGFSPDIVSAIGFATPPCVSRKLAESC 360

Query: 361 ADYVTTVVMQDDVIPRLSVASLTRLRIEILQTDWMSVIEKQDLKGIVGLVTNAKQVVTSV 420
           ADYVTTVVMQDD+IPRLS+ASLT+LR+EILQTDW SVIE+QD K I GL+TNAKQVVTSV
Sbjct: 361 ADYVTTVVMQDDIIPRLSLASLTKLRVEILQTDWTSVIEEQDWKNIYGLLTNAKQVVTSV 420

Query: 421 QDVARKLADFAKFTSKKRSSDDSNRKESHAASGPPSSHPTVALQSAAAAENEAAKCKIPE 480
           QDVARKL D+AKFTSKK  SDDS  K SH ASGPP S PT  +Q A  A+NEAA+CKIPE
Sbjct: 421 QDVARKLGDYAKFTSKKGPSDDSYGKGSHVASGPPPSQPTALVQRADTAQNEAARCKIPE 480

Query: 481 ELFVPGTVYYLKRH--MDSTPEYFTLWKRHPDEHFQQVVLSSNLISNHKCDSHYYALRDV 540
           ELFVPGTVYYLK+H  ++   EYFTLWKRHPDEHFQQ+VLSSNLIS+HKCDSHY+ALRDV
Sbjct: 481 ELFVPGTVYYLKKHVNLEEAYEYFTLWKRHPDEHFQQIVLSSNLISDHKCDSHYHALRDV 540

Query: 541 LKSLPSCNDEEGIFS 554
           LK LPSCND EGIFS
Sbjct: 541 LKGLPSCND-EGIFS 551

[Close]

BLAST of Lag0001992 vs. ExPASy TrEMBL
Match: A0A6J1KT65 (sn1-specific diacylglycerol lipase beta OS=Cucurbita maxima OX=3661 GN=LOC111498003 PE=4 SV=1)

HSP 1 Score: 880.6 bits (2274), Expect = 3.5e-252
Identity = 463/555 (83.42%), Postives = 493/555 (88.83%), Query Frame = 0


Query: 1   MASRTARRLLHNFHYARSFLRKQEWQASSVTVNSVLLHFQRSKADKVGQGVHKEGEVLPA 60
           M++ TARRLL N + ARS+LRKQ+WQASSVTVNSVL+H QRSKAD V   V KE EVLP 
Sbjct: 1   MSNGTARRLLRNLYSARSYLRKQKWQASSVTVNSVLMHLQRSKADMVENNVRKENEVLPE 60

Query: 61  VSVRLEMENTSKKGSKPTKYFSDAEDISDSNRRLELAWLTVTKALEPALQLFRWALSSGD 120
           VSVR EME   KKGSK  KYFSDAEDISDSNRRLELAWL  TKAL+ A QL+RWALSSGD
Sbjct: 61  VSVRSEMEKNIKKGSKTPKYFSDAEDISDSNRRLELAWL--TKALKSASQLYRWALSSGD 120

Query: 121 EIQNQIPPRSRSVSEIIASIQRSKMGIQDWSLSDLTIGLCLIYLRQASTNPLEDIKGVQI 180
            IQNQI PRSRSVSEIIASIQRSKMGIQ+WSLSDLTIGL LIYLRQASTNPL DIKGVQI
Sbjct: 121 GIQNQIQPRSRSVSEIIASIQRSKMGIQEWSLSDLTIGLFLIYLRQASTNPLADIKGVQI 180

Query: 181 SSDAIVEDLIYYLELADGSYKDSAAMLARRTMLRECNILKFVKDSSVMRPGYYIGVDTRK 240
           +S+AIVEDLIYYLELA G YKDSAAMLAR TMLRE NILKF+KDSSVMRPGYYIGVDTRK
Sbjct: 181 TSNAIVEDLIYYLELAKGCYKDSAAMLARNTMLREPNILKFIKDSSVMRPGYYIGVDTRK 240

Query: 241 KLVIFGIRGTRTVYDLITDIITSSDREEVTFEGYSTHFGTSESARWFLDHEIGMIRRCLE 300
           KLVIFGIRGT +VYDLITDI TSSD EEVTFEGYSTHFGTSESARWFLDHEI MIR+CL+
Sbjct: 241 KLVIFGIRGTHSVYDLITDITTSSD-EEVTFEGYSTHFGTSESARWFLDHEIEMIRQCLK 300

Query: 301 KYQGFRLRLVGHSLGGATASLLAVMLRKMSNKELGFSPDIVSAIGFATPPCVSRKLAESC 360
           KYQGFRLRLVGHSLGGA ASLLAVMLRK SNKELGF PDIVSAIGFATPPCVSRKLAESC
Sbjct: 301 KYQGFRLRLVGHSLGGAIASLLAVMLRKKSNKELGFRPDIVSAIGFATPPCVSRKLAESC 360

Query: 361 ADYVTTVVMQDDVIPRLSVASLTRLRIEILQTDWMSVIEKQDLKGIVGLVTNAKQVVTSV 420
           ADYVTTVVMQDD+IPRLS+ASLT+LRIEILQTDW SVIE+QD K I GL+TNAKQVVTSV
Sbjct: 361 ADYVTTVVMQDDIIPRLSLASLTKLRIEILQTDWTSVIEEQDWKNIYGLLTNAKQVVTSV 420

Query: 421 QDVARKLADFAKFTSKKRSSDDSNRKESHAASGPPSSHPTVALQSAAAAENEAAKCKIPE 480
           QDVARKL D+AKFTSKK  SDDS  K SH ASGPP S PT  +Q A  A+NEAA+CKIPE
Sbjct: 421 QDVARKLGDYAKFTSKKGPSDDSYGKGSHVASGPPPSQPTAVVQRADTAQNEAARCKIPE 480

Query: 481 ELFVPGTVYYLKRH--MDSTPEYFTLWKRHPDEHFQQVVLSSNLISNHKCDSHYYALRDV 540
           ELFVPGTVYYLK+H  ++   EYFTLWKRHPDEHFQQ+VLSSNLIS+HKCDSHY+ALRDV
Sbjct: 481 ELFVPGTVYYLKKHVNLEEAYEYFTLWKRHPDEHFQQIVLSSNLISDHKCDSHYHALRDV 540

Query: 541 LKSLPSCNDEEGIFS 554
           LK LPSCND EGIFS
Sbjct: 541 LKGLPSCND-EGIFS 551

[Close]

BLAST of Lag0001992 vs. ExPASy TrEMBL
Match: A0A6J1DSG2 (uncharacterized protein LOC111023479 OS=Momordica charantia OX=3673 GN=LOC111023479 PE=4 SV=1)

HSP 1 Score: 866.7 bits (2238), Expect = 5.3e-248
Identity = 460/554 (83.03%), Postives = 491/554 (88.63%), Query Frame = 0


Query: 1   MASRTARRLLHNFHYARSFLRKQEWQASSVTVNSVLLHFQRSKADKVGQGVHKEGEVLPA 60
           MAS   RRLLHNFH+ARSFLRKQE QASSVTVNSVL+HF RSKADKV  GVHKEGEVLP 
Sbjct: 1   MASGAVRRLLHNFHHARSFLRKQELQASSVTVNSVLMHFHRSKADKVEIGVHKEGEVLPE 60

Query: 61  VSVRLEMENTSKKGSKPTKYFSDAEDISDSNRRLELAWLTVTKALEPALQLFRWALS-SG 120
           VSVR EME  SKKGS+PTKYFSD E+ISDS RR ELAWL  TKALEPALQL+RWALS SG
Sbjct: 61  VSVRSEMEKNSKKGSEPTKYFSDDENISDSKRRSELAWL--TKALEPALQLYRWALSASG 120

Query: 121 DEIQNQIPPRSRSVSEIIASIQRSKMGIQDWSLSDLTIGLCLIYLRQASTNPLEDIKGVQ 180
           D I++Q PPRSRSVSEIIASIQ SKMGIQDWSLSDLTIGL LIYLRQASTNP EDI GVQ
Sbjct: 121 DGIRDQTPPRSRSVSEIIASIQWSKMGIQDWSLSDLTIGLYLIYLRQASTNPFEDISGVQ 180

Query: 181 ISSDAIVEDLIYYLELADGSYKDSAAMLARRTMLRECNILKFVKDSSVMRPGYYIGVDTR 240
           ISSDAIV+DLIYYLELA GSYKDSAA LAR +MLRECNILKFV DSSVMRPGYYIGVDTR
Sbjct: 181 ISSDAIVQDLIYYLELAKGSYKDSAASLARNSMLRECNILKFVGDSSVMRPGYYIGVDTR 240

Query: 241 KKLVIFGIRGTRTVYDLITDIITSSDREEVTFEGYSTHFGTSESARWFLDHEIGMIRRCL 300
           KKLVIFGIRGT T+ DLITDI+TSSD  EVTFEGY THFGT+ESARWFLDHE+GMIRRCL
Sbjct: 241 KKLVIFGIRGTHTLSDLITDIVTSSD-GEVTFEGYVTHFGTAESARWFLDHEMGMIRRCL 300

Query: 301 EKYQGFRLRLVGHSLGGATASLLAVMLRKMSNKELGFSPDIVSAIGFATPPCVSRKLAES 360
           EKYQGFRLRLVGHSLGGA ASLLAVMLRK SNKELGFSPDIVSAIG+ATPPCVSRKLAES
Sbjct: 301 EKYQGFRLRLVGHSLGGAIASLLAVMLRKKSNKELGFSPDIVSAIGYATPPCVSRKLAES 360

Query: 361 CADYVTTVVMQDDVIPRLSVASLTRLRIEILQTDWMSVIEKQDLKGIVGLVTNAKQVVTS 420
           CADYVTTVVMQDD+IPRLS+ASLTRLR EILQTDW SVIEKQD K  +GLVTNA+QVV+S
Sbjct: 361 CADYVTTVVMQDDIIPRLSIASLTRLRNEILQTDWTSVIEKQDWKSFIGLVTNARQVVSS 420

Query: 421 VQDVARKLADFAKFTSKKRSSDDSNRKESHAASGPPSSHPTVALQSAAAAENEAAKCKIP 480
           VQDVARKLAD+AKFTSKK SSD S RKESH AS PP  HPT A ++ AA     A+ K P
Sbjct: 421 VQDVARKLADYAKFTSKKISSDGSIRKESHVASAPP--HPTAAARNKAA----TARYKTP 480

Query: 481 EELFVPGTVYYLKRHMDSTPEYFTLWKRHPDEHFQQVVLSSNLISNHKCDSHYYALRDVL 540
           EELFVPGTVYYLKR+ + TPEYFTLWKRHP EHFQ++VLSSNLIS+H+CDSHYYALRDVL
Sbjct: 481 EELFVPGTVYYLKRNTNGTPEYFTLWKRHPGEHFQRIVLSSNLISDHRCDSHYYALRDVL 540

Query: 541 KSLPSCNDEEGIFS 554
           K LP+ +D E IFS
Sbjct: 541 KGLPARSD-ESIFS 544

[Close]

BLAST of Lag0001992 vs. ExPASy TrEMBL
Match: A0A5A7TF50 (Alpha/beta-Hydrolases superfamily protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold67G005250 PE=4 SV=1)

HSP 1 Score: 850.9 bits (2197), Expect = 3.0e-243
Identity = 446/550 (81.09%), Postives = 490/550 (89.09%), Query Frame = 0


Query: 1   MASRTARRLLHNFHYARSFLRKQEWQASSVTVNSVLLHFQRSKADKVGQGVHKEGEVLPA 60
           MA+ TARRL+HNFHYAR+FLRKQEWQA SVTVN+VL+HFQ SK DKV   V KEGEVL  
Sbjct: 1   MATGTARRLIHNFHYARNFLRKQEWQARSVTVNNVLMHFQSSKVDKVENDVIKEGEVLRE 60

Query: 61  VSVRLEMENTSKKGSKPTKYFSDAEDISDSNRRLELAWLTVTKALEPALQLFRWALSSGD 120
           VS+R EME  S    K ++Y    ED +DSN R ELAWL  TK LEPALQL+RWALSSGD
Sbjct: 61  VSLRSEMEKNSTIDCKRSEY---VEDSTDSNWRSELAWL--TKTLEPALQLYRWALSSGD 120

Query: 121 EIQNQIPPRSRSVSEIIASIQRSKMGIQDWSLSDLTIGLCLIYLRQASTNPLEDIKGVQI 180
                IP RSRSVSEIIASIQRSK GIQDWSLSDLTIGLCLIYLRQASTNPLED+KGVQI
Sbjct: 121 ----GIPHRSRSVSEIIASIQRSKTGIQDWSLSDLTIGLCLIYLRQASTNPLEDLKGVQI 180

Query: 181 SSDAIVEDLIYYLELADGSYKDSAAMLARRTMLRECNILKFVKDSSVMRPGYYIGVDTRK 240
           SS+AIVEDLIY++ELA+GSYK+S +MLA+ TMLRECNILKFVK+SSVMRPGYYIGVDTRK
Sbjct: 181 SSNAIVEDLIYFVELAEGSYKNSTSMLAKTTMLRECNILKFVKNSSVMRPGYYIGVDTRK 240

Query: 241 KLVIFGIRGTRTVYDLITDIITSSDREEVTFEGYSTHFGTSESARWFLDHEIGMIRRCLE 300
           KLVIFGIRGT TVYDLITDIIT+SDR +VTFEGYSTHFGTSESA+WFL +EIGMIRRCLE
Sbjct: 241 KLVIFGIRGTHTVYDLITDIITTSDR-DVTFEGYSTHFGTSESAQWFLQNEIGMIRRCLE 300

Query: 301 KYQGFRLRLVGHSLGGATASLLAVMLRKMSNKELGFSPDIVSAIGFATPPCVSRKLAESC 360
           KYQGFRLRLVGHSLGGA ASLLAVMLRK S KELGFSPDIVSAIGF TPPCVSRKLAESC
Sbjct: 301 KYQGFRLRLVGHSLGGAIASLLAVMLRKKSKKELGFSPDIVSAIGFGTPPCVSRKLAESC 360

Query: 361 ADYVTTVVMQDDVIPRLSVASLTRLRIEILQTDWMSVIEKQDLKGIVGLVTNAKQVVTSV 420
           ADYVTTVVMQDDVIPRLSVASLTRLRIEILQTDWMS+I K+D K I+GLVTNAKQVVTSV
Sbjct: 361 ADYVTTVVMQDDVIPRLSVASLTRLRIEILQTDWMSLIGKEDWKSIIGLVTNAKQVVTSV 420

Query: 421 QDVARKLADFAKFTSKKRSSDDSNRKESHAASGPPSSHPTVALQSAAAAENEAAKCKIPE 480
           QDVA+KLAD+AKFTSKK+SSDD+N+KES  ASG P S  T ALQSA AA+N+AA+CKI +
Sbjct: 421 QDVAQKLADYAKFTSKKKSSDDNNKKESDVASGSPRSCATSALQSATAAQNKAARCKISD 480

Query: 481 ELFVPGTVYYLKRHMDSTPEYFTLWKRHPDEHFQQVVLSSNLISNHKCDSHYYALRDVLK 540
           ELF+PGTVYYLKRH+DSTPEYF+LWKRHPDEHFQQ+VLS+ L+S+HKCDSHYYALRDVLK
Sbjct: 481 ELFIPGTVYYLKRHVDSTPEYFSLWKRHPDEHFQQIVLSNILLSDHKCDSHYYALRDVLK 540

Query: 541 SLP--SCNDE 549
            LP  SC++E
Sbjct: 541 GLPISSCSNE 540

[Close]

BLAST of Lag0001992 vs. ExPASy TrEMBL
Match: A0A1S3BHV5 (uncharacterized protein LOC103489737 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103489737 PE=4 SV=1)

HSP 1 Score: 850.9 bits (2197), Expect = 3.0e-243
Identity = 446/551 (80.94%), Postives = 491/551 (89.11%), Query Frame = 0


Query: 1   MASRTARRLLHNFHYARSFLRKQEWQASSVTVNSVLLHFQRSKADKVGQGVHKEGEVLPA 60
           MA+ TARRL+HNFHYAR+FLRKQEWQA SVTVN+VL+HFQ SK DKV   V+KEGEVL  
Sbjct: 1   MATGTARRLIHNFHYARNFLRKQEWQARSVTVNNVLMHFQSSKVDKVENDVNKEGEVLRE 60

Query: 61  VSVRLEMENTSKKGSKPTKYFSDAEDISDSNRRLELAWLTVTKALEPALQLFRWALSSGD 120
           VS+R EM   S    K ++Y    ED +DSN R ELAWL  TKALEPALQL+RWALSSGD
Sbjct: 61  VSLRSEMGKNSTIDCKRSEY---VEDTTDSNWRSELAWL--TKALEPALQLYRWALSSGD 120

Query: 121 EIQNQIPPRSRSVSEIIASIQRSKMGIQDWSLSDLTIGLCLIYLRQASTNPLEDIKGVQI 180
                IP RSRSVSEIIASIQRSK GIQDWSLSDLTIGLCLIYLRQASTNPLED+KGVQI
Sbjct: 121 ----GIPHRSRSVSEIIASIQRSKTGIQDWSLSDLTIGLCLIYLRQASTNPLEDLKGVQI 180

Query: 181 SSDAIVEDLIYYLELADGSYKDSAAMLARRTMLRECNILKFVKDSSVMRPGYYIGVDTRK 240
           SS+AIVEDLIY++ELA+GSYK+S +MLA+ TMLRECNILKFVK+SSVMRPGYYIGVDTRK
Sbjct: 181 SSNAIVEDLIYFVELAEGSYKNSTSMLAKTTMLRECNILKFVKNSSVMRPGYYIGVDTRK 240

Query: 241 KLVIFGIRGTRTVYDLITDIITSSDREEVTFEGYSTHFGTSESARWFLDHEIGMIRRCLE 300
           KLVIFGIRGT TVYDLITDIIT+SDR +VTFEGYSTHFGTSESA+WFL +EIGMIRRCLE
Sbjct: 241 KLVIFGIRGTHTVYDLITDIITTSDR-DVTFEGYSTHFGTSESAQWFLQNEIGMIRRCLE 300

Query: 301 KYQGFRLRLVGHSLGGATASLLAVMLRKMSNKELGFSPDIVSAIGFATPPCVSRKLAESC 360
           KYQGFRLRLVGHSLGGA ASLLAVMLRK S KELGFSPDIVSAIGF TPPCVSRKLAESC
Sbjct: 301 KYQGFRLRLVGHSLGGAIASLLAVMLRKKSKKELGFSPDIVSAIGFGTPPCVSRKLAESC 360

Query: 361 ADYVTTVVMQDDVIPRLSVASLTRLRIEILQTDWMSVIEKQDLKGIVGLVTNAKQVVTSV 420
           ADYVTTVVMQDDVIPRLSVASLTRLRIEILQTDWMS+I K+D K I+ LVTNAKQVVTSV
Sbjct: 361 ADYVTTVVMQDDVIPRLSVASLTRLRIEILQTDWMSLIGKEDWKSIIDLVTNAKQVVTSV 420

Query: 421 QDVARKLADFAKFTSKKRSSDDSNRKESHAASGPPSSHPTVALQSAAAAENEAAKCKIPE 480
           QDVA+KLAD+AKFTSKK+SSDD+N+KES  ASG P S  T ALQSA AA+N+AA+CKI +
Sbjct: 421 QDVAQKLADYAKFTSKKKSSDDNNKKESDVASGSPRSCATSALQSATAAQNKAARCKISD 480

Query: 481 ELFVPGTVYYLKRHMDSTPEYFTLWKRHPDEHFQQVVLSSNLISNHKCDSHYYALRDVLK 540
           ELF+PGTVYYLKRH+DSTPEYF+LWKRHPDEHFQQ+VLS+ L+S+HKCDSHYYALRDVLK
Sbjct: 481 ELFIPGTVYYLKRHVDSTPEYFSLWKRHPDEHFQQIVLSNILLSDHKCDSHYYALRDVLK 540

Query: 541 SLP--SCNDEE 550
            LP  SC++EE
Sbjct: 541 GLPISSCSNEE 541

[Close]

BLAST of Lag0001992 vs. TAIR 10
Match: AT2G42450.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 585.5 bits (1508), Expect = 4.5e-167
Identity = 323/566 (57.07%), Postives = 404/566 (71.38%), Query Frame = 0


Query: 1   MASRTARRLLHNFHYARSFLRKQEWQASSVTVNSVLLHFQRSKADKVGQGVHKEGEVLPA 60
           MA+R   RLL NF    S  RK+      V  N V+  FQ S   + G+ V KE E   A
Sbjct: 1   MANRALNRLLRNFFVESSPFRKRSGLYQGVVYN-VIKRFQ-SHLSESGK-VEKELEEQEA 60

Query: 61  VSVRLEMENTSKKGSKPTKYF--------SDAEDISDSNRRLELAWLTVTKALEPALQLF 120
              R     +S+ G +  + F        S  ED  D   +L+LAWL  TKALEPA+QL 
Sbjct: 61  EKRRPSSVTSSRSGLEKRRQFESGIDNDSSSDEDTGDGKWKLDLAWL--TKALEPAMQLR 120

Query: 121 RWALSSGDEIQNQIPPRSRSVSEIIASIQRSKMGIQDWSLSDLTIGLCLIYLRQASTNPL 180
           RWAL +G++      P SRS+SEIIASIQRS++GI+ W+  DLTIGL LIYLRQAS +P 
Sbjct: 121 RWALPTGEKSL----PGSRSLSEIIASIQRSRLGIEGWTFGDLTIGLYLIYLRQASLSPF 180

Query: 181 EDIKGVQISSDAIVEDLIYYLELADGSYKDSAAMLARRTMLRECNILKFVKDSSVMRPGY 240
           ED+KGV++ S++ V DLIY  ELA G Y+DS + LA+ TMLRE NILKFVKDSSVMRPGY
Sbjct: 181 EDVKGVEVVSESTVYDLIYNAELAKGCYRDSVSGLAKNTMLRENNILKFVKDSSVMRPGY 240

Query: 241 YIGVDTRKKLVIFGIRGTRTVYDLITDIITSSDREEVTFEGYSTHFGTSESARWFLDHEI 300
           YIGVD R+KLV+FGIRGT T+YDLITDI++SSD EEVTFEGYSTHFGT+E+ARWFL+HE+
Sbjct: 241 YIGVDHRRKLVVFGIRGTHTIYDLITDIVSSSD-EEVTFEGYSTHFGTAEAARWFLNHEL 300

Query: 301 GMIRRCLEKYQGFRLRLVGHSLGGATASLLAVMLRKMSNKELGFSPDIVSAIGFATPPCV 360
             IRRCL KY+G++LRLVGHSLGGA ASL+A+ML+KM  +ELGF  +I+SA+G+ATPPCV
Sbjct: 301 QTIRRCLAKYEGYKLRLVGHSLGGAIASLMAIMLKKMPREELGFDAEIISAVGYATPPCV 360

Query: 361 SRKLAESCADYVTTVVMQDDVIPRLSVASLTRLRIEILQTDWMSVIEKQDLKGIVGLVTN 420
           S++LAE+C+++VTT+VMQDD+IPRLS ASL RLR EILQTDW SVIEK++ K ++ LVTN
Sbjct: 361 SKELAENCSEFVTTIVMQDDIIPRLSAASLARLRNEILQTDWTSVIEKEEWKNVLDLVTN 420

Query: 421 AKQVVTSVQDVARKLADFAKFTSKKRSSDDSNRKESHAASGPPSSHPTVALQSAAAAENE 480
           AKQVVTSVQDVARK++D+A F +KK   +  + K +               QS     + 
Sbjct: 421 AKQVVTSVQDVARKVSDYANFGNKKEVPEIPSSKNN---------------QSGILISDS 480

Query: 481 AAK--CKIPEELFVPGTVYYLKRHMDSTP--------EYFTLWKRHPDEHFQQVVLSSNL 540
             K   K+PEEL+VPG VYYL R +  TP        EY++LWKR P +HFQ+++LS N 
Sbjct: 481 TTKDVVKLPEELYVPGAVYYLMRSLRGTPKSSAGKQVEYYSLWKRDPGQHFQRILLSGNF 540

Query: 541 ISNHKCDSHYYALRDVLKSLPSCNDE 549
           I++HKCDSHYYALRDVLK  PS  +E
Sbjct: 541 ITDHKCDSHYYALRDVLKGFPSFINE 541

[Close]

BLAST of Lag0001992 vs. TAIR 10
Match: AT4G16070.1 (Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 )

HSP 1 Score: 99.8 bits (247), Expect = 7.5e-21
Identity = 74/272 (27.21%), Postives = 130/272 (47.79%), Query Frame = 0


Query: 150 WSLSDLTIGLCLIYLRQ---------ASTNPLEDIKGVQISSDAIVEDLIYYLELADGSY 209
           W ++DL  G+  +  RQ         A +N +E +KG +I  D  + +L+ +L L     
Sbjct: 81  WPIADLAFGINYLMRRQGNFPTASVYAGSNCIE-LKGPEIIMD--LTELLRFLTLCMLFS 140

Query: 210 KDSAAMLARRTMLRECNILKFVKDSSVMRPGYYIGVDTRKKLVIFGIRGTRTVYDLIT-- 269
           K   A+          ++L     + +M+P + I  DT  K ++  IRGT ++ D +T  
Sbjct: 141 KKPFAVFLESAGYTHEDVLLQKPKAGIMQPAFTIIRDTNSKCILLLIRGTHSIKDTLTAA 200

Query: 270 ---------DIITSSDREEVTFEGYSTHFGTSESARWFLDHEIGMIRRCLEKYQGFRLRL 329
                     ++       +   GY+ H G   +ARW     +  + + L++   F++++
Sbjct: 201 TGAVVPFHHSVLHDGGLSNLVL-GYA-HCGMVAAARWIAKLSVPCLLKALDENPSFKVQI 260

Query: 330 VGHSLGGATASLLAVMLRKMSNKELGFSPDIVSAIGFATPPCVSRKLAESCADYVTTVVM 389
           VGHSLGG TASLL  +LR+   KE   +    +   FA   C++  LAES   ++TT++ 
Sbjct: 261 VGHSLGGGTASLLTYILRE--QKEFASA----TCFTFAPAACMTWDLAESGKHFITTIIN 320

Query: 390 QDDVIPRLSVASLTRLRIEILQTDWMSVIEKQ 402
             D++P  S +S+  LR E+  + W + +  Q
Sbjct: 321 GSDLVPTFSASSVDDLRSEVTSSSWSNDLRDQ 341

[Close]

BLAST of Lag0001992 vs. TAIR 10
Match: AT4G16070.2 (Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 )

HSP 1 Score: 94.7 bits (234), Expect = 2.4e-19
Identity = 71/272 (26.10%), Postives = 125/272 (45.96%), Query Frame = 0


Query: 150 WSLSDLTIGLCLIYLRQ---------ASTNPLEDIKGVQISSDAIVEDLIYYLELADGSY 209
           W ++DL  G+  +  RQ         A +N +E +KG +I  D  + +L+ +L L     
Sbjct: 81  WPIADLAFGINYLMRRQGNFPTASVYAGSNCIE-LKGPEIIMD--LTELLRFLTLCMLFS 140

Query: 210 KDSAAMLARRTMLRECNILKFVKDSSVMRPGYYIGVDTRKKLVIFGIRGTRTVYDLIT-- 269
           K   A+          ++L     + +M+P + I  DT  K ++  IRGT ++ D +T  
Sbjct: 141 KKPFAVFLESAGYTHEDVLLQKPKAGIMQPAFTIIRDTNSKCILLLIRGTHSIKDTLTAA 200

Query: 270 ---------DIITSSDREEVTFEGYSTHFGTSESARWFLDHEIGMIRRCLEKYQGFRLRL 329
                     ++       +   GY+ H G   +ARW     +  + + L++   F++++
Sbjct: 201 TGAVVPFHHSVLHDGGLSNLVL-GYA-HCGMVAAARWIAKLSVPCLLKALDENPSFKVQI 260

Query: 330 VGHSLGGATASLLAVMLRKMSNKELGFSPDIVSAIGFATPPCVSRKLAESCADYVTTVVM 389
           VGHSLGG TASLL  +LR+                 FA+  C +   AES   ++TT++ 
Sbjct: 261 VGHSLGGGTASLLTYILREQKE--------------FASATCFTFAPAESGKHFITTIIN 320

Query: 390 QDDVIPRLSVASLTRLRIEILQTDWMSVIEKQ 402
             D++P  S +S+  LR E+  + W + +  Q
Sbjct: 321 GSDLVPTFSASSVDDLRSEVTSSSWSNDLRDQ 333

[Close]

BLAST of Lag0001992 vs. TAIR 10
Match: AT3G14075.1 (Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 )

HSP 1 Score: 92.0 bits (227), Expect = 1.6e-18
Identity = 67/267 (25.09%), Postives = 120/267 (44.94%), Query Frame = 0


Query: 150 WSLSDLTIGLCLIYLRQASTNPLEDIKG---VQISSDAIVEDLIYYLELADGSY---KDS 209
           W + DL  G+  +  RQ   +      G   V++    +  +L Y L L    +   K S
Sbjct: 83  WPIGDLAFGINFLIKRQGLLHVDRVFGGKDSVELRGSEVATELKYLLHLLTLCWHFSKKS 142

Query: 210 AAMLARRTMLRECNILKFVKDSSVMRPGYYIGVDTRKKLVIFGIRGTRTVYDLI---TDI 269
                  T   + N+L     + +++P + + VD   K  +  IRGT ++ D +   T  
Sbjct: 143 FPFFLEETGFTKENVLIHEPKAGILKPAFTVLVDHNTKYFLLLIRGTHSIKDTLTAATGA 202

Query: 270 ITSSDREEVTFEGYS------THFGTSESARWFLDHEIGMIRRCLEKYQGFRLRLVGHSL 329
           I       V   G S       H G   +AR         + + LE+Y  +++++VGHSL
Sbjct: 203 IVPFHHTVVNERGVSNLVLGYAHCGMVAAARCIAKLATPCLLKGLEQYPDYKIKIVGHSL 262

Query: 330 GGATASLLAVMLRKMSNKELGFSPDIVSAIGFATPPCVSRKLAESCADYVTTVVMQDDVI 389
           GG TA+LL  ++R+             + + FA   C++ +LA+S  D++ +V+   D++
Sbjct: 263 GGGTAALLTYIMREQK------MLSTATCVTFAPAACMTWELADSGNDFIVSVINGADLV 322

Query: 390 PRLSVASLTRLRIEILQTDWMSVIEKQ 402
           P  S A++  LR E+  + W++ +  Q
Sbjct: 323 PTFSAAAVDDLRAEVTASAWLNDLRNQ 343

[Close]

BLAST of Lag0001992 vs. TAIR 10
Match: AT3G14075.2 (Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 )

HSP 1 Score: 92.0 bits (227), Expect = 1.6e-18
Identity = 67/267 (25.09%), Postives = 120/267 (44.94%), Query Frame = 0


Query: 150 WSLSDLTIGLCLIYLRQASTNPLEDIKG---VQISSDAIVEDLIYYLELADGSY---KDS 209
           W + DL  G+  +  RQ   +      G   V++    +  +L Y L L    +   K S
Sbjct: 83  WPIGDLAFGINFLIKRQGLLHVDRVFGGKDSVELRGSEVATELKYLLHLLTLCWHFSKKS 142

Query: 210 AAMLARRTMLRECNILKFVKDSSVMRPGYYIGVDTRKKLVIFGIRGTRTVYDLI---TDI 269
                  T   + N+L     + +++P + + VD   K  +  IRGT ++ D +   T  
Sbjct: 143 FPFFLEETGFTKENVLIHEPKAGILKPAFTVLVDHNTKYFLLLIRGTHSIKDTLTAATGA 202

Query: 270 ITSSDREEVTFEGYS------THFGTSESARWFLDHEIGMIRRCLEKYQGFRLRLVGHSL 329
           I       V   G S       H G   +AR         + + LE+Y  +++++VGHSL
Sbjct: 203 IVPFHHTVVNERGVSNLVLGYAHCGMVAAARCIAKLATPCLLKGLEQYPDYKIKIVGHSL 262

Query: 330 GGATASLLAVMLRKMSNKELGFSPDIVSAIGFATPPCVSRKLAESCADYVTTVVMQDDVI 389
           GG TA+LL  ++R+             + + FA   C++ +LA+S  D++ +V+   D++
Sbjct: 263 GGGTAALLTYIMREQK------MLSTATCVTFAPAACMTWELADSGNDFIVSVINGADLV 322

Query: 390 PRLSVASLTRLRIEILQTDWMSVIEKQ 402
           P  S A++  LR E+  + W++ +  Q
Sbjct: 323 PTFSAAAVDDLRAEVTASAWLNDLRNQ 343

The following BLAST results are available for this feature:

BLAST of Lag0001992 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038897252.1	7.0e-255	84.12	uncharacterized protein LOC120085373 isoform X1 [Benincasa hispida]	[more]
XP_023513693.1	1.3e-253	83.78	sn1-specific diacylglycerol lipase beta [Cucurbita pepo subsp. pepo]	[more]
XP_022959891.1	1.5e-252	83.60	sn1-specific diacylglycerol lipase beta [Cucurbita moschata]	[more]
XP_023004816.1	7.3e-252	83.42	sn1-specific diacylglycerol lipase beta [Cucurbita maxima]	[more]
XP_022156622.1	1.1e-247	83.03	uncharacterized protein LOC111023479 [Momordica charantia]	[more]

BLAST of Lag0001992 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P0C1S9	2.3e-14	33.94	Diacylglycerol lipase-beta OS=Rattus norvegicus OX=10116 GN=Daglb PE=1 SV=1	[more]
Q91WC9	1.1e-13	30.21	Diacylglycerol lipase-beta OS=Mus musculus OX=10090 GN=Daglb PE=1 SV=2	[more]
Q8NCG7	1.5e-13	33.33	Diacylglycerol lipase-beta OS=Homo sapiens OX=9606 GN=DAGLB PE=1 SV=2	[more]
Q9Y4D2	4.2e-08	30.00	Diacylglycerol lipase-alpha OS=Homo sapiens OX=9606 GN=DAGLA PE=1 SV=3	[more]
Q6WQJ1	4.2e-08	30.00	Diacylglycerol lipase-alpha OS=Mus musculus OX=10090 GN=Dagla PE=1 SV=2	[more]

BLAST of Lag0001992 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H649	7.1e-253	83.60	sn1-specific diacylglycerol lipase beta OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1KT65	3.5e-252	83.42	sn1-specific diacylglycerol lipase beta OS=Cucurbita maxima OX=3661 GN=LOC111498...	[more]
A0A6J1DSG2	5.3e-248	83.03	uncharacterized protein LOC111023479 OS=Momordica charantia OX=3673 GN=LOC111023...	[more]
A0A5A7TF50	3.0e-243	81.09	Alpha/beta-Hydrolases superfamily protein OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A1S3BHV5	3.0e-243	80.94	uncharacterized protein LOC103489737 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10...	[more]

BLAST of Lag0001992 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G42450.1	4.5e-167	57.07	alpha/beta-Hydrolases superfamily protein	[more]
AT4G16070.1	7.5e-21	27.21	Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3	[more]
AT4G16070.2	2.4e-19	26.10	Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3	[more]
AT3G14075.1	1.6e-18	25.09	Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3	[more]
AT3G14075.2	1.6e-18	25.09	Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002921	Fungal lipase-like domain	PFAM	PF01764	Lipase_3	coord: 245..379 e-value: 3.0E-22 score: 79.0
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 175..422 e-value: 3.4E-34 score: 120.2
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 187..380
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 437..461
None	No IPR available	PANTHER	PTHR47418	ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN	coord: 101..548
None	No IPR available	CDD	cd00519	Lipase_3	coord: 189..384 e-value: 2.26082E-39 score: 141.46

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0001992.1	Lag0001992.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
molecular_function	GO:0016787	hydrolase activity