Lag0000532 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0000532 vs. NCBI nr
Match: XP_023552915.1 (uncharacterized protein LOC111810441 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 123.2 bits (308), Expect = 2.8e-24
Identity = 72/201 (35.82%), Postives = 119/201 (59.20%), Query Frame = 0

Query: 1   MFISRVYYMKDPDWNHWKEKFIDELPSYVSERVYMTLRERFDGNINWSSLTYDDLTSYVI 60
           M+ S+V    D     WKE F++ELP + S R+   L+ +++G I W +L+Y  + S++I
Sbjct: 138 MYFSKVLIRTDSSLEFWKENFVNELPKHFSRRIKDGLKTKYNGTIPWQTLSYGSIASFII 197

Query: 61  NCGLHICNEIKVQRKVQKNEKSRLRDMKAFCSQYGCERIEEPSRPSNKIIRRRNSRRYYK 120
             GL +CNE K+Q K+  +  +R +++  FC QYGC+ IE PS       RR+  + + K
Sbjct: 198 EEGLRLCNESKIQNKLNSSVSNR-KELGRFCDQYGCKGIEAPSTS-----RRKKFKTHPK 257

Query: 121 NQYNDRP-QFYQRQPRKYIPRKQYTPNPRYKKRQSRHPAKPNQTTYRNKGKTFCFKCQKE 180
             ++ RP + Y+ +P +       +  P Y +R+   P KP    ++ K +  CFKC++E
Sbjct: 258 PYHSYRPRETYRNKPVQ-------SQKPTYSRRKYT-PTKP----HKGKKRQTCFKCREE 317

Query: 181 GHYANRCPTR-RINELDLEEE 200
           GHYAN+CP R +INEL++++E
Sbjct: 318 GHYANKCPIRGKINELEIDQE 320

BLAST of Lag0000532 vs. NCBI nr
Match: XP_023522280.1 (uncharacterized protein LOC111786173, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 121.3 bits (303), Expect = 1.1e-23
Identity = 80/227 (35.24%), Postives = 119/227 (52.42%), Query Frame = 0

Query: 1   MFISRVYYMKDPDWNHWKEKFIDELPSYVSERVYMTLRERFDGNINWSSLTYDDLTSYVI 60
           M++S+V    D     WKE F++ LP + S R+   L+ +++G I W +L+Y  + S++I
Sbjct: 129 MYLSKVLIRTDSSLEFWKENFVNGLPKHFSRRIKDGLKTKYNGTIPWQTLSYGSIASFII 188

Query: 61  NCGLHICNEIKVQRKVQKNEKSRLRDMKAFCSQYGCERIEEPSRPSNKIIRRRNSRRYYK 120
             GL +CNE K+Q K+  +  S  +++  FC QYGC+ IE PS        RR   + + 
Sbjct: 189 EEGLRLCNESKIQNKL--SSISNRKELGRFCDQYGCKGIEAPS------TSRRKKVKTHP 248

Query: 121 NQYNDRPQFYQRQPRKYIPRKQYTPNP--RYKKRQSRHPAKPNQTTYRNKGKTFCFKCQK 180
             YN            Y PR++Y   P    K   SR    P + T+R K K  CFKC++
Sbjct: 249 KPYN-----------SYRPREKYRNKPVQSQKPTYSRRKYTPTK-THRGKKKQTCFKCRE 308

Query: 181 EGHYANRCPTR-RINELDLEEEYSSDY---------DSSDQELNEFQ 216
           EGHYAN CP R +INELD+++E  +            SS+ E+ E Q
Sbjct: 309 EGHYANECPIRGKINELDIDQELKNQLLRLALTDSEQSSEGEILELQ 335

BLAST of Lag0000532 vs. NCBI nr
Match: XP_022930200.1 (uncharacterized protein LOC111436716 [Cucurbita moschata])

HSP 1 Score: 119.4 bits (298), Expect = 4.0e-23
Identity = 71/199 (35.68%), Postives = 116/199 (58.29%), Query Frame = 0

Query: 1   MFISRVYYMKDPDWNHWKEKFIDELPSYVSERVYMTLRERFDGNINWSSLTYDDLTSYVI 60
           ++ S+V    D     WKE F++ LP + S R+   L+ +++G I W +L+Y  + S++I
Sbjct: 129 IYFSKVLIRTDSSLEFWKENFVNGLPKHFSRRIKDGLKTKYNGTILWQTLSYRSIASFII 188

Query: 61  NCGLHICNEIKVQRKVQKNEKSRLRDMKAFCSQYGCERIEEPSRPSNKIIRRRNSRRYYK 120
             GL +CNE K+Q K+  +  +R +++  FC QYGC+ IE PS       RR+  + + K
Sbjct: 189 EEGLRLCNESKIQNKLNSSISNR-KELGRFCDQYGCKEIEAPSTS-----RRKKVKTHPK 248

Query: 121 NQYNDRP-QFYQRQPRKYIPRKQYTPNPRYKKRQSRHPAKPNQTTYRNKGKTFCFKCQKE 180
             ++ RP + Y+ +P +       +  P Y +R+   P KP    ++ K +  CFKC++E
Sbjct: 249 PYHSYRPREAYRNKPVQ-------SQKPTYSRRRYT-PTKP----HKGKKRKTCFKCREE 308

Query: 181 GHYANRCPTR-RINELDLE 198
           GHYAN+CP R +INELD+E
Sbjct: 309 GHYANKCPIRGKINELDIE 309

BLAST of Lag0000532 vs. NCBI nr
Match: XP_022974313.1 (uncharacterized protein LOC111472952 [Cucurbita maxima])

HSP 1 Score: 117.9 bits (294), Expect = 1.2e-22
Identity = 69/200 (34.50%), Postives = 112/200 (56.00%), Query Frame = 0

Query: 1   MFISRVYYMKDPDWNHWKEKFIDELPSYVSERVYMTLRERFDGNINWSSLTYDDLTSYVI 60
           M+ ++V    +     WKE F++ LP Y S R+   L+ +++G I W + +Y+ + S++I
Sbjct: 66  MYFNKVLIRTNSSLEFWKENFVNGLPKYFSRRIKDGLKTKYNGTIPWQTFSYESIVSFII 125

Query: 61  NCGLHICNEIKVQRKVQKNEKSRLRDMKAFCSQYGCERIEEPSRPSNKIIRRRNSRRYYK 120
             GL +CNE K+Q K+  +   + +++  FC QYGC+ I+ PS        RR   + Y+
Sbjct: 126 E-GLRLCNESKIQNKLSSSISDK-KELGRFCDQYGCKGIKAPS------TSRRKKVKTYQ 185

Query: 121 NQYNDRPQFYQRQPRKYIPRKQYTPNPRYKKRQSRHPAKPNQTTYRNKGKTFCFKCQKEG 180
             YN       R   KY  +   +  P Y K++   P K    T+R K +  CFKC++EG
Sbjct: 186 KPYNS-----YRPREKYRSKPMQSQKPTYSKKRYT-PTK----THRGKKRQACFKCREEG 245

Query: 181 HYANRCPTR-RINELDLEEE 200
           HYAN+CP + +INELD+++E
Sbjct: 246 HYANKCPIKGKINELDIDQE 247

BLAST of Lag0000532 vs. NCBI nr
Match: XP_023520850.1 (uncharacterized protein LOC111784362 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 117.1 bits (292), Expect = 2.0e-22
Identity = 70/200 (35.00%), Postives = 113/200 (56.50%), Query Frame = 0

Query: 1   MFISRVYYMKDPDWNHWKEKFIDELPSYVSERVYMTLRERFDGNINWSSLTYDDLTSYVI 60
           M+ S+V    D     WKE F++ LP + S R+   L+ +++G I W +L+Y  + S++I
Sbjct: 447 MYFSKVLIRTDSSLEFWKENFVNGLPKHFSRRIKDGLKTKYNGTIPWQTLSYGSIASFII 506

Query: 61  NCGLHICNEIKVQRKVQKNEKSRLRDMKAFCSQYGCERIEEPSRPSNKIIRRRNSRRYYK 120
             GL +CNE K+Q K+  +  +R +++  FC QYGC+ IE P     K  R++  + + K
Sbjct: 507 EEGLRLCNESKIQNKLNSSISNR-KELGRFCDQYGCKGIEAP-----KTSRQKKVKTHPK 566

Query: 121 NQYNDRPQFYQRQPRKYIPRKQYTPNPRYKKRQSRHPAKPNQTTYRNKGKTFCFKCQKEG 180
             ++ RP+        Y  +   +  P Y +R+   P K    T+R K K  CFKC+ EG
Sbjct: 567 PYHSYRPR------EAYRSKPVQSQKPTYSRRK-YIPTK----THRGKKKQTCFKCRVEG 626

Query: 181 HYANRCPTR-RINELDLEEE 200
           HYA +CP + +INELD+++E
Sbjct: 627 HYAKKCPIKGKINELDIDQE 629

BLAST of Lag0000532 vs. ExPASy TrEMBL
Match: A0A6J1EWB4 (uncharacterized protein LOC111436716 OS=Cucurbita moschata OX=3662 GN=LOC111436716 PE=4 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 1.9e-23
Identity = 71/199 (35.68%), Postives = 116/199 (58.29%), Query Frame = 0

Query: 1   MFISRVYYMKDPDWNHWKEKFIDELPSYVSERVYMTLRERFDGNINWSSLTYDDLTSYVI 60
           ++ S+V    D     WKE F++ LP + S R+   L+ +++G I W +L+Y  + S++I
Sbjct: 129 IYFSKVLIRTDSSLEFWKENFVNGLPKHFSRRIKDGLKTKYNGTILWQTLSYRSIASFII 188

Query: 61  NCGLHICNEIKVQRKVQKNEKSRLRDMKAFCSQYGCERIEEPSRPSNKIIRRRNSRRYYK 120
             GL +CNE K+Q K+  +  +R +++  FC QYGC+ IE PS       RR+  + + K
Sbjct: 189 EEGLRLCNESKIQNKLNSSISNR-KELGRFCDQYGCKEIEAPSTS-----RRKKVKTHPK 248

Query: 121 NQYNDRP-QFYQRQPRKYIPRKQYTPNPRYKKRQSRHPAKPNQTTYRNKGKTFCFKCQKE 180
             ++ RP + Y+ +P +       +  P Y +R+   P KP    ++ K +  CFKC++E
Sbjct: 249 PYHSYRPREAYRNKPVQ-------SQKPTYSRRRYT-PTKP----HKGKKRKTCFKCREE 308

Query: 181 GHYANRCPTR-RINELDLE 198
           GHYAN+CP R +INELD+E
Sbjct: 309 GHYANKCPIRGKINELDIE 309

BLAST of Lag0000532 vs. ExPASy TrEMBL
Match: A0A6J1EW44 (uncharacterized protein LOC111436618 OS=Cucurbita moschata OX=3662 GN=LOC111436618 PE=4 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 1.6e-22
Identity = 77/225 (34.22%), Postives = 122/225 (54.22%), Query Frame = 0

Query: 1   MFISRVYYMKDPDWNHWKEKFIDELPSYVSERVYMTLRERFDGNINWSSLTYDDLTSYVI 60
           M+ S+V    D     WKE F++ LP + S R+   L+ +++G I W +L+Y  + S +I
Sbjct: 17  MYFSKVLIRTDSSLEFWKENFVNGLPKHFSRRIKDGLKTKYNGTIPWQTLSYGSIASVII 76

Query: 61  NCGLHICNEIKVQRKVQKNEKSRLRDMKAFCSQYGCERIEEPSRPSNKIIRRRNSRRYYK 120
             GL +CNE K+Q K+  +  +R +++  FC QYGC+ IE PS       RR+  + + K
Sbjct: 77  EEGLRLCNESKIQNKLNSSISNR-KELGRFCDQYGCKGIEAPSTS-----RRKKVKTHPK 136

Query: 121 NQYNDRPQFYQRQPRKYIPRKQYTPNPRYKKRQSRHPAKPNQTTYRNKGKTFCFKCQKEG 180
             ++ RP+        Y+ +   +  PRY +R+   P K    T+R K K   FKC++EG
Sbjct: 137 PYHSYRPR------ETYLNKPVQSQKPRYSRRK-YIPTK----THRGKKKQTSFKCREEG 196

Query: 181 HYANRCPTR-RINELDLEEEYSSDY---------DSSDQELNEFQ 216
           HY N+CP R +INELD+++E  +            SS+ E+ E Q
Sbjct: 197 HYVNKCPIRGKINELDIDQELKNQLLRLTLTDSEQSSEGEILELQ 224

BLAST of Lag0000532 vs. ExPASy TrEMBL
Match: A0A6J1EYM2 (uncharacterized protein LOC111439730 OS=Cucurbita moschata OX=3662 GN=LOC111439730 PE=4 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 1.8e-21
Identity = 78/230 (33.91%), Postives = 118/230 (51.30%), Query Frame = 0

Query: 1   MFISRVYYMKDPDWNHWKEKFIDELPSYVSERVYMTLRERFDGNINWSSLTYDDLTSYVI 60
           M+ S+V    D     WKE F++ LP + S R+   L+ + +G I W +L+Y  + S++I
Sbjct: 17  MYFSKVLIRTDSSLEFWKENFVNGLPKHFSRRIKDGLKTKHNGTIPWQTLSYGSIASFII 76

Query: 61  NCGLHICNEIKVQRKVQKNEKSRLRDMKAFCSQYGCERIEEPSRPSNKIIRRRNSRRYYK 120
             GL + NE K+Q K+  +  +R +++  FC QYGC+ IE PS                +
Sbjct: 77  EEGLRLYNESKIQNKLNSSISNR-KELGRFCDQYGCKGIEAPSTS-------------IR 136

Query: 121 NQYNDRPQFYQRQPRKYIPRKQYTPNPRYKKRQSRHPAKPNQ-----TTYRNKGKTFCFK 180
           N+    P+ Y      Y PR+ Y    R K  QS+ PA   +      T+  K K  CFK
Sbjct: 137 NKVKTHPKPY----HSYRPRETY----RNKPVQSQKPAYSRRKYIPTKTHGGKKKQTCFK 196

Query: 181 CQKEGHYANRCPTR-RINELDLEEEYSSDY---------DSSDQELNEFQ 216
           C++EGHYAN+CP R +INELD+++E  +            SS+ E+ E Q
Sbjct: 197 CREEGHYANKCPIRGKINELDIDQELKNQLLPLTLTDSEQSSEGEILELQ 224

BLAST of Lag0000532 vs. ExPASy TrEMBL
Match: A0A151R9C8 (Polyprotein OS=Cajanus cajan OX=3821 GN=KK1_039609 PE=4 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 1.5e-20
Identity = 69/236 (29.24%), Postives = 124/236 (52.54%), Query Frame = 0

Query: 1   MFISRVYYMKDPDWNHWKEKFIDELPSYVSERVYMTLRERFDGNINWSSLTYDDLTSYVI 60
           +F+S++   +D     WKE+FI  LP   SE+V M L   +   I +++LTY  + S ++
Sbjct: 361 VFMSQITLREDGYQGFWKERFISGLPKLFSEKVKMKLESHYGKPIPYNTLTYGQIHSIIV 420

Query: 61  NCGLHICNEIKVQRKVQKNEKSRLRDMKAFCSQYGCERIEEPSRPSNKIIRRRNSRRYYK 120
           + G+ +CN+ K+Q K++K   +  R++  FC QYG   I  PS      ++++  +R + 
Sbjct: 421 HTGIQVCNDFKLQNKLRKEASTNKREIGNFCEQYGLAPIRAPS-----AVKKKTQKRQFI 480

Query: 121 NQYNDRPQFYQRQPRKYIPRKQYTPNPRYKKRQSRHPAKPNQTTYRNKGKTFCFKCQKEG 180
           +Q + +  +Y++  +K         N  YKK  S++  KP++    +K    C+KC + G
Sbjct: 481 SQKSYQKPYYKKPYKK-------PYNKTYKKNYSKNIEKPHKKFNNSKKNVTCWKCGRTG 540

Query: 181 HYANRCPT-RRINELDL-----------------EEEYSSDYDSSDQELNEFQFND 219
           H+AN+C   ++IN+L++                 EEE +SD D  +QE+ E   N+
Sbjct: 541 HFANKCKVQQKINQLEIDENLKKTLLTIMINSESEEEETSDEDEKEQEVKEIDLNE 584

BLAST of Lag0000532 vs. ExPASy TrEMBL
Match: A0A392M7U4 (CCHC-type domain-containing protein (Fragment) OS=Trifolium medium OX=97028 GN=A2U01_0004383 PE=4 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 8.4e-19
Identity = 84/242 (34.71%), Postives = 134/242 (55.37%), Query Frame = 0

Query: 2   FISRVYYMKDPDWNHWKEKFIDELPSYVSERVYMTLRERFDG-NINWSSLTYDDLTSYVI 61
           F++RV+ +K+P+  HWK KFID LP   +E+V  +LR + DG NIN++ LTY  + S  I
Sbjct: 240 FLTRVFQIKEPNTTHWKSKFIDGLPHLFAEKVRHSLRNKNDGININYADLTYGQIISTCI 299

Query: 62  NCGLHICNEIKVQRKVQKNEKSRLRDMKAFCSQYGCERIEEPSRPSNKIIRRRNSRR--- 121
           N GL +CN+IK++ +++K + +    +  FC Q+  + +++P  P NK   R N  R   
Sbjct: 300 NEGLSLCNDIKLKNQLKKQKLTEKHQIGEFCEQFAFD-LDKP--PGNKGRERGNKPRSGK 359

Query: 122 ---YYKNQYNDRPQFYQRQPRKYIPRKQYTPNPRYKKRQSRHPAKPNQTTYRNKGKTF-- 181
              Y+K  YN  P  Y+++      R++  P+P+ +K         N T  + K K    
Sbjct: 360 KNPYHKKDYNKYP--YKKR------RRKTNPDPKIRKY--------NITPKKRKAKKLDI 419

Query: 182 -CFKCQKEGHYANRCPTRR-INELDLEEEYSSDYD-----SSDQELN----EFQF-NDTS 223
            C KC K GHYAN+C  +R +NE++ ++   +  +     SSD ELN    E  F +D+S
Sbjct: 420 TCHKCGKPGHYANQCRVKRALNEIE-DDNLRNQLEKILLISSDSELNISPDEVDFISDSS 461