Lag0000323 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0000323
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionnon-specific lipid-transfer protein 2-like
Locationchr4: 3994025 .. 3994333 (-)
RNA-Seq ExpressionLag0000323
SyntenyLag0000323
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATAAACTTCCTATCACAGTTGTTTGGCTCTTTGCGGTGGCAGTAATGGTGGCAGTTCTCACCGGAGCTGGTCTGGCGGGTGCACAAACAACTTGTGACACTGCGCAGTTGAGTCCATGTTTCAATGCTTACGTATTGGGTCAAAAACCAAGCACGAAGTGTTGCAATAAGTTGAAGGAGCAACAACCATGCTACTGTACGTACCTCAAAAATCCTAGTTTGCAGCCTTTTATAGATTCCGATCGCGCAAGAGCGATTGCTGAAGCTTGTGACATCACCTTTCCATCTGAGAAGGATTGTCCGTAG

mRNA sequence

ATGAATAAACTTCCTATCACAGTTGTTTGGCTCTTTGCGGTGGCAGTAATGGTGGCAGTTCTCACCGGAGCTGGTCTGGCGGGTGCACAAACAACTTGTGACACTGCGCAGTTGAGTCCATGTTTCAATGCTTACGTATTGGGTCAAAAACCAAGCACGAAGTGTTGCAATAAGTTGAAGGAGCAACAACCATGCTACTGTACGTACCTCAAAAATCCTAGTTTGCAGCCTTTTATAGATTCCGATCGCGCAAGAGCGATTGCTGAAGCTTGTGACATCACCTTTCCATCTGAGAAGGATTGTCCGTAG

Coding sequence (CDS)

ATGAATAAACTTCCTATCACAGTTGTTTGGCTCTTTGCGGTGGCAGTAATGGTGGCAGTTCTCACCGGAGCTGGTCTGGCGGGTGCACAAACAACTTGTGACACTGCGCAGTTGAGTCCATGTTTCAATGCTTACGTATTGGGTCAAAAACCAAGCACGAAGTGTTGCAATAAGTTGAAGGAGCAACAACCATGCTACTGTACGTACCTCAAAAATCCTAGTTTGCAGCCTTTTATAGATTCCGATCGCGCAAGAGCGATTGCTGAAGCTTGTGACATCACCTTTCCATCTGAGAAGGATTGTCCGTAG

Protein sequence

MNKLPITVVWLFAVAVMVAVLTGAGLAGAQTTCDTAQLSPCFNAYVLGQKPSTKCCNKLKEQQPCYCTYLKNPSLQPFIDSDRARAIAEACDITFPSEKDCP
Homology
BLAST of Lag0000323 vs. NCBI nr
Match: XP_008444079.1 (PREDICTED: non-specific lipid-transfer protein 2P-like [Cucumis melo] >KAA0064163.1 non-specific lipid-transfer protein 2P-like [Cucumis melo var. makuwa] >TYK02866.1 non-specific lipid-transfer protein 2P-like [Cucumis melo var. makuwa])

HSP 1 Score: 113.2 bits (282), Expect = 1.3e-21
Identity = 54/103 (52.43%), Postives = 70/103 (67.96%), Query Frame = 0

Query: 1   MNKLPIT-VVWLFAVAVMVAVLTGAGLAGAQTTCDTAQLSPCFNAYVLGQKPSTKCCNKL 60
           M   PIT VV +F VA  VA+L G     AQT CD +QLS C  A++ G  P+ +CC KL
Sbjct: 1   MKNFPITLVVCIFVVATTVALLNGPPPVAAQTVCDASQLSSCAPAFLFGGNPNQECCTKL 60

Query: 61  KEQQPCYCTYLKNPSLQPFIDSDRARAIAEACDITFPSEKDCP 103
           + +QPCYC Y  +P+LQ F++SD AR +AEAC+ITFP+  DCP
Sbjct: 61  RAEQPCYCEYFGDPNLQNFVNSDAARRVAEACNITFPTMADCP 103

BLAST of Lag0000323 vs. NCBI nr
Match: KAG7026200.1 (hypothetical protein SDJN02_12699, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 108.6 bits (270), Expect = 3.2e-20
Identity = 58/103 (56.31%), Postives = 69/103 (66.99%), Query Frame = 0

Query: 1   MNKLPITVVWLFAVAVMVAVLTGAGLAGAQTTCDTAQLS-PCFNAYVLGQKPSTKCCNKL 60
           M K  ITVV  F    MVA LTGA   GAQ TCD   L+ PC  A+  G +PST+CCNKL
Sbjct: 1   MKKFGITVVCFF----MVAFLTGAHHVGAQQTCDPQLLAIPCGLAF-SGMRPSTRCCNKL 60

Query: 61  KEQQPCYCTYLKNPSLQPFIDSDRARAIAEACDITFPSEKDCP 103
           +EQQPCYC YL NP L+ F+DS  AR IA  C+IT P++ +CP
Sbjct: 61  REQQPCYCAYLNNPDLKGFVDSPAARRIARDCNITIPTQAECP 98

BLAST of Lag0000323 vs. NCBI nr
Match: KAG6581747.1 (hypothetical protein SDJN03_21749, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 107.1 bits (266), Expect = 9.3e-20
Identity = 57/103 (55.34%), Postives = 69/103 (66.99%), Query Frame = 0

Query: 1   MNKLPITVVWLFAVAVMVAVLTGAGLAGAQTTCDTAQLS-PCFNAYVLGQKPSTKCCNKL 60
           M K  ITVV  F    +VA LTGA   GAQ TCD   L+ PC  A+  G +PST+CCNKL
Sbjct: 1   MKKFGITVVCFF----VVAFLTGAHHVGAQQTCDPQLLAIPCGLAF-SGMRPSTRCCNKL 60

Query: 61  KEQQPCYCTYLKNPSLQPFIDSDRARAIAEACDITFPSEKDCP 103
           +EQQPCYC YL NP L+ F+DS  AR IA  C+IT P++ +CP
Sbjct: 61  REQQPCYCAYLNNPDLKGFVDSPAARRIARDCNITIPTQAECP 98

BLAST of Lag0000323 vs. NCBI nr
Match: KAE8648021.1 (hypothetical protein Csa_005848 [Cucumis sativus])

HSP 1 Score: 101.7 bits (252), Expect = 3.9e-18
Identity = 52/102 (50.98%), Postives = 67/102 (65.69%), Query Frame = 0

Query: 1   MNKLPIT-VVWLFAVAVMVAVLTGAGLAGAQTTCDTAQLSPCFNAYVLGQKPSTKCCNKL 60
           M   PIT VV +F VA  VA+L GA    AQ  CD +QLS C  A+  G  PS  CCNKL
Sbjct: 1   MKNFPITIVVCIFVVATTVALLNGAPPVVAQVECDPSQLSSCTAAF-FGMTPSQTCCNKL 60

Query: 61  KEQQPCYCTYLKNPSLQPFIDSDRARAIAEACDITFPSEKDC 102
           +E QPCYC Y+ +P L+ F++S  AR IA++C+I+ P+E DC
Sbjct: 61  REAQPCYCEYINDPDLRSFVNSSAARRIAKSCNISLPTEADC 101

BLAST of Lag0000323 vs. NCBI nr
Match: XP_022143278.1 (non-specific lipid-transfer protein 2-like [Momordica charantia])

HSP 1 Score: 100.1 bits (248), Expect = 1.1e-17
Identity = 50/97 (51.55%), Postives = 65/97 (67.01%), Query Frame = 0

Query: 1  MNKLPITVVWLFAVAVMVAVLTGAGLAGAQTTCDTAQLSPCFNAYVLGQKPSTKCCNKLK 60
          M  LPIT +WL  VA+M A+LTGA +A A  TC   QLS C  A     +PS  CC+KL+
Sbjct: 1  MKTLPITGLWLLVVAIM-ALLTGAHVAEA-VTCSPLQLSSCAGAISSSTRPSASCCSKLR 60

Query: 61 EQQPCYCTYLKNPSLQPFIDSDRARAIAEACDITFPS 98
          EQ+PC C YLKNP+L+P++ S  A+ +A AC + FPS
Sbjct: 61 EQRPCLCGYLKNPNLRPYVQSPGAKTVASACGVAFPS 95

BLAST of Lag0000323 vs. ExPASy Swiss-Prot
Match: Q43681 (Probable non-specific lipid-transfer protein AKCS9 OS=Vigna unguiculata OX=3917 PE=2 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 4.6e-14
Identity = 36/95 (37.89%), Postives = 56/95 (58.95%), Query Frame = 0

Query: 3  KLPITVVWLFAVAVMVAVLTGAGLAGAQTTCDTAQLSPCFNAYVLGQKPSTKCCNKLKEQ 62
          K+ + +  + AV V+   L   G      TC+  +LS C  A   G KPS+ CC+KLK Q
Sbjct: 4  KMKMKMSVVCAVVVVALFLIDVGPVAEAVTCNPTELSSCVPAITGGSKPSSTCCSKLKVQ 63

Query: 63 QPCYCTYLKNPSLQPFIDSDRARAIAEACDITFPS 98
          +PC C Y+KNPSL+ +++S  A+ +   C +T+P+
Sbjct: 64 EPCLCNYIKNPSLKQYVNSPGAKKVLSNCGVTYPN 98

BLAST of Lag0000323 vs. ExPASy Swiss-Prot
Match: P86809 (Non-specific lipid-transfer protein 2 OS=Apium graveolens var. rapaceum OX=278110 PE=1 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 8.8e-13
Identity = 31/62 (50.00%), Postives = 40/62 (64.52%), Query Frame = 0

Query: 32 TCDTAQLSPCFNAYVLGQKPSTKCCNKLKEQQPCYCTYLKNPSLQPFIDSDRARAIAEAC 91
          TC   QLSPC  A      PS  CCNKLKEQ+PC C YLK+P+L+ +++S  AR  A +C
Sbjct: 2  TCSAVQLSPCLAAITKNTPPSAACCNKLKEQKPCLCGYLKDPNLKNYVNSPGARKTASSC 61

Query: 92 DI 94
           +
Sbjct: 62 GV 63

BLAST of Lag0000323 vs. ExPASy Swiss-Prot
Match: P82353 (Non-specific lipid-transfer protein 2 OS=Prunus armeniaca OX=36596 PE=1 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 1.5e-12
Identity = 31/65 (47.69%), Postives = 39/65 (60.00%), Query Frame = 0

Query: 32 TCDTAQLSPCFNAYVLGQKPSTKCCNKLKEQQPCYCTYLKNPSLQPFIDSDRARAIAEAC 91
          TC   QLSPC      G    T CC KL+EQ+PC C YLKNPSL+ +++S  AR +A  C
Sbjct: 2  TCSPVQLSPCLGPINSGAPSPTTCCQKLREQRPCLCGYLKNPSLRQYVNSPNARKLASNC 61

Query: 92 DITFP 97
           +  P
Sbjct: 62 GVPVP 66

BLAST of Lag0000323 vs. ExPASy Swiss-Prot
Match: P20145 (Probable non-specific lipid-transfer protein OS=Hordeum vulgare OX=4513 GN=LTP2 PE=2 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 6.3e-11
Identity = 33/81 (40.74%), Postives = 44/81 (54.32%), Query Frame = 0

Query: 16  VMVAVLTGAGLAGAQTTCDTAQLSPCFNAYVLGQKPSTKCCNKLKEQQPCYCTYLKNPSL 75
           VMV  L     AGA   C+ AQL+ C +A + G KPS +CC  L+ QQ C C Y+K+P+ 
Sbjct: 20  VMVVTLAAGADAGAGAACEPAQLAVCASAILGGTKPSGECCGNLRAQQGCLCQYVKDPNY 79

Query: 76  QPFIDSDRARAIAEACDITFP 97
             ++ S  AR     C I  P
Sbjct: 80  GHYVSSPHARDTLNLCGIPVP 100

BLAST of Lag0000323 vs. ExPASy Swiss-Prot
Match: P82900 (Non-specific lipid-transfer protein 2G OS=Triticum aestivum OX=4565 PE=1 SV=2)

HSP 1 Score: 65.5 bits (158), Expect = 4.1e-10
Identity = 32/83 (38.55%), Postives = 48/83 (57.83%), Query Frame = 0

Query: 15 AVMVA-VLTGAGLAGAQTTCDTAQLSPCFNAYVLGQKPSTKCCNKLKEQQPCYCTYLKNP 74
          A+M+A V+  A   GA+  C  +QL+ C +A + G KPS +CC  L+ QQ C+C Y K+P
Sbjct: 12 ALMLALVVLAAAPGGARAACQASQLAVCASAILSGAKPSGECCGNLRAQQGCFCQYAKDP 71

Query: 75 SLQPFIDSDRARAIAEACDITFP 97
          +   +I S  AR    +C +  P
Sbjct: 72 TYGQYIRSPHARDTLTSCGLAVP 94

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008444079.11.3e-2152.43PREDICTED: non-specific lipid-transfer protein 2P-like [Cucumis melo] >KAA006416... [more]
KAG7026200.13.2e-2056.31hypothetical protein SDJN02_12699, partial [Cucurbita argyrosperma subsp. argyro... [more]
KAG6581747.19.3e-2055.34hypothetical protein SDJN03_21749, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAE8648021.13.9e-1850.98hypothetical protein Csa_005848 [Cucumis sativus][more]
XP_022143278.11.1e-1751.55non-specific lipid-transfer protein 2-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q436814.6e-1437.89Probable non-specific lipid-transfer protein AKCS9 OS=Vigna unguiculata OX=3917 ... [more]
P868098.8e-1350.00Non-specific lipid-transfer protein 2 OS=Apium graveolens var. rapaceum OX=27811... [more]
P823531.5e-1247.69Non-specific lipid-transfer protein 2 OS=Prunus armeniaca OX=36596 PE=1 SV=1[more]
P201456.3e-1140.74Probable non-specific lipid-transfer protein OS=Hordeum vulgare OX=4513 GN=LTP2 ... [more]
P829004.1e-1038.55Non-specific lipid-transfer protein 2G OS=Triticum aestivum OX=4565 PE=1 SV=2[more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036312Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamilyGENE3D1.10.110.10coord: 31..98
e-value: 5.8E-22
score: 79.7
IPR036312Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamilySUPERFAMILY47699Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumincoord: 32..97
IPR016140Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domainPFAMPF14368LTP_2coord: 14..101
e-value: 4.4E-10
score: 39.6
IPR033872Non-specific lipid-transfer protein type 2PANTHERPTHR33214BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEINcoord: 11..97
IPR033872Non-specific lipid-transfer protein type 2CDDcd01959nsLTP2coord: 33..97
e-value: 9.79489E-21
score: 76.5834
NoneNo IPR availablePANTHERPTHR33214:SF52NON-SPECIFIC LIPID-TRANSFER PROTEIN 2coord: 11..97

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0000323.1Lag0000323.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006869 lipid transport