IVF0025385 (gene) Melon (IVF77) v1

Overview
NameIVF0025385
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Descriptionprotein MIZU-KUSSEI 1-like
Locationchr08: 13161134 .. 13162395 (+)
RNA-Seq ExpressionIVF0025385
SyntenyIVF0025385
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCTCATTTCCTGTCATCTTCTTATTTATTCTTCCCTTCTCTCTCTACAAAATGAACATTCTCTCTCCATTCCTTCTCCACTAACTCTATCATTCTTCATCCTTCTTTTCTCTCTCATCGCCATGACAATCCTCCAAAGAACACGAAGTAGCAGTGCCGCCATCAAAGCCACCACAAAATTAATCCCATCTTCCATCTCCGGCCACCGCCACCACGACTTCAGATCCTTCTCCTCCTCTAATCTGCCGCCGGAGTATTCCAATCCAAACAATTTTTCCGGCCACCATGGCCACCGCCACCAAACGGTAACGGGATCAAAATTCTCTATCCTTCTCCACTCACTTCTCAAGCTCCTCTCGGTTCCCACTTGTAAATGGCTCTCCATCCCCACCCACCTCTCCGTTAGGCCGTCTCTTGGCCGGAGAGTTGTCACTGGAACACTCTTTGGTCGACGCCGTGGCCACGTCACCTTTGCGGTTCAAATACACCCCCAATCCGAGCCGGTTTTGTTGCTTGAGTTGGCCACTTCTACTTCATCCCTAGTCAAGGAAATGTCCTCCGGTTTGGTTCGGATAGCCCTCGAATCTGAGGCACCTGCCTATAATGGTATGAAATTAAGGACAATGATCAGTGTATCCTTATGACGCACTATCTCAAATGTTCCATGTTTTAATCTTGTGTGGCGGAGGGATGGAATCATCTACCCACCAAAAATTTGTTTCTTAAATTACTAGCCATTCTAAAAGTATTTTTCTATTTTCCCCCTTTATTTTAAATAGTTTTAAATAGTTTTAGATAATTTCAATTTAAATAACCTTTTATCCATATTCTTTAACAGATGGTCGACCAAAGAAGTTGTTTGAGGAGCCCAGATGGACGATGTATTGTAACGGTAGAAAGTGTGGGCACTCGATGTTGCGTACGTGTGGAGAGTTTGATCGGTATGTGTTGAACATGGTCCAGAGTGTGTCGGTCGGTGCCGGAGTTATTCCAGTGGTGGAGAACGGCGGAAAAAGCAGTAGGTCAGAGGGAGAGTTACTTTACATGAGAGCAAAGTTTGAGCGAGTTGTAGGTAGCCGTGACTCGGAAGCTTTTTACATGATAAATCCAGATAGTAATGGAGGACCTGAACTCAGTATTTTTCTTCTAAGAATATAATTTTTCCAATAAGAAAAAGCCCACAAAAATAGATGGATTAACTTCTATTTCTGACTTCTATCGTTTACTTATTTTAATTAAATGTATAATAACTCAAATTCAAG

mRNA sequence

CCCTCATTTCCTGTCATCTTCTTATTTATTCTTCCCTTCTCTCTCTACAAAATGAACATTCTCTCTCCATTCCTTCTCCACTAACTCTATCATTCTTCATCCTTCTTTTCTCTCTCATCGCCATGACAATCCTCCAAAGAACACGAAGTAGCAGTGCCGCCATCAAAGCCACCACAAAATTAATCCCATCTTCCATCTCCGGCCACCGCCACCACGACTTCAGATCCTTCTCCTCCTCTAATCTGCCGCCGGAGTATTCCAATCCAAACAATTTTTCCGGCCACCATGGCCACCGCCACCAAACGGTAACGGGATCAAAATTCTCTATCCTTCTCCACTCACTTCTCAAGCTCCTCTCGGTTCCCACTTGTAAATGGCTCTCCATCCCCACCCACCTCTCCGTTAGGCCGTCTCTTGGCCGGAGAGTTGTCACTGGAACACTCTTTGGTCGACGCCGTGGCCACGTCACCTTTGCGGTTCAAATACACCCCCAATCCGAGCCGGTTTTGTTGCTTGAGTTGGCCACTTCTACTTCATCCCTAGTCAAGGAAATGTCCTCCGGTTTGGTTCGGATAGCCCTCGAATCTGAGGCACCTGCCTATAATGATGGTCGACCAAAGAAGTTGTTTGAGGAGCCCAGATGGACGATGTATTGTAACGGTAGAAAGTGTGGGCACTCGATGTTGCGTACGTGTGGAGAGTTTGATCGGTATGTGTTGAACATGGTCCAGAGTGTGTCGGTCGGTGCCGGAGTTATTCCAGTGGTGGAGAACGGCGGAAAAAGCAGTAGGTCAGAGGGAGAGTTACTTTACATGAGAGCAAAGTTTGAGCGAGTTGTAGGTAGCCGTGACTCGGAAGCTTTTTACATGATAAATCCAGATAGTAATGGAGGACCTGAACTCAGTATTTTTCTTCTAAGAATATAATTTTTCCAATAAGAAAAAGCCCACAAAAATAGATGGATTAACTTCTATTTCTGACTTCTATCGTTTACTTATTTTAATTAAATGTATAATAACTCAAATTCAAG

Coding sequence (CDS)

ATGACAATCCTCCAAAGAACACGAAGTAGCAGTGCCGCCATCAAAGCCACCACAAAATTAATCCCATCTTCCATCTCCGGCCACCGCCACCACGACTTCAGATCCTTCTCCTCCTCTAATCTGCCGCCGGAGTATTCCAATCCAAACAATTTTTCCGGCCACCATGGCCACCGCCACCAAACGGTAACGGGATCAAAATTCTCTATCCTTCTCCACTCACTTCTCAAGCTCCTCTCGGTTCCCACTTGTAAATGGCTCTCCATCCCCACCCACCTCTCCGTTAGGCCGTCTCTTGGCCGGAGAGTTGTCACTGGAACACTCTTTGGTCGACGCCGTGGCCACGTCACCTTTGCGGTTCAAATACACCCCCAATCCGAGCCGGTTTTGTTGCTTGAGTTGGCCACTTCTACTTCATCCCTAGTCAAGGAAATGTCCTCCGGTTTGGTTCGGATAGCCCTCGAATCTGAGGCACCTGCCTATAATGATGGTCGACCAAAGAAGTTGTTTGAGGAGCCCAGATGGACGATGTATTGTAACGGTAGAAAGTGTGGGCACTCGATGTTGCGTACGTGTGGAGAGTTTGATCGGTATGTGTTGAACATGGTCCAGAGTGTGTCGGTCGGTGCCGGAGTTATTCCAGTGGTGGAGAACGGCGGAAAAAGCAGTAGGTCAGAGGGAGAGTTACTTTACATGAGAGCAAAGTTTGAGCGAGTTGTAGGTAGCCGTGACTCGGAAGCTTTTTACATGATAAATCCAGATAGTAATGGAGGACCTGAACTCAGTATTTTTCTTCTAAGAATATAA

Protein sequence

MTILQRTRSSSAAIKATTKLIPSSISGHRHHDFRSFSSSNLPPEYSNPNNFSGHHGHRHQTVTGSKFSILLHSLLKLLSVPTCKWLSIPTHLSVRPSLGRRVVTGTLFGRRRGHVTFAVQIHPQSEPVLLLELATSTSSLVKEMSSGLVRIALESEAPAYNDGRPKKLFEEPRWTMYCNGRKCGHSMLRTCGEFDRYVLNMVQSVSVGAGVIPVVENGGKSSRSEGELLYMRAKFERVVGSRDSEAFYMINPDSNGGPELSIFLLRI
Homology
BLAST of IVF0025385 vs. ExPASy Swiss-Prot
Match: O22227 (Protein MIZU-KUSSEI 1 OS=Arabidopsis thaliana OX=3702 GN=MIZ1 PE=1 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 3.6e-58
Identity = 146/282 (51.77%), Postives = 182/282 (64.54%), Query Frame = 0

Query: 8   RSSSAAIKATTKLIPSSISGHRHHDFRSFSSSNLPPEYSNPNNFSGHHGHRHQTVTGSKF 67
           RSS     +++ LIP SI  H          S +P   S+ ++ S + G  H      KF
Sbjct: 28  RSSHVRSPSSSALIP-SIPEHELFLVPCRRCSYVP--LSSSSSASHNIGKFH-----LKF 87

Query: 68  SILLHSLLKLLSVPTCKWLSIPTHLSV---------------RPSLGRRVVTGTLFGRRR 127
           S+LL S + ++++P CK LS+P+  S                  SLGRR VTGTL+G +R
Sbjct: 88  SLLLRSFINIINIPACKMLSLPSPPSSSSSVSNQLISLVTGGSSSLGRR-VTGTLYGHKR 147

Query: 128 GHVTFAVQIHPQSEPVLLLELATSTSSLVKEMSSGLVRIALESEAPAYNDGRPKKLFEEP 187
           GHVTF+VQ + +S+PVLLL+LA ST++LVKEMSSGLVRIALE E   +  G   KLF+EP
Sbjct: 148 GHVTFSVQYNQRSDPVLLLDLAMSTATLVKEMSSGLVRIALECE-KRHRSG--TKLFQEP 207

Query: 188 RWTMYCNGRKCGHSMLR--TCGEFDRYVLNMVQSVSVGAGVIPVVE-----NGGKSSRSE 247
           +WTMYCNGRKCG+++ R   C + D  VLN V  V+VGAGVIP  +     +G  S    
Sbjct: 208 KWTMYCNGRKCGYAVSRGGACTDTDWRVLNTVSRVTVGAGVIPTPKTIDDVSGVGSGTEL 267

Query: 248 GELLYMRAKFERVVGSRDSEAFYMINPDSNGGPELSIFLLRI 268
           GELLYMR KFERVVGSRDSEAFYM+NPD NGGPELSIFLLRI
Sbjct: 268 GELLYMRGKFERVVGSRDSEAFYMMNPDKNGGPELSIFLLRI 297

BLAST of IVF0025385 vs. ExPASy TrEMBL
Match: A0A5A7SPQ9 (Protein MIZU-KUSSEI 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1702G00210 PE=4 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 1.9e-147
Identity = 267/267 (100.00%), Postives = 267/267 (100.00%), Query Frame = 0

Query: 1   MTILQRTRSSSAAIKATTKLIPSSISGHRHHDFRSFSSSNLPPEYSNPNNFSGHHGHRHQ 60
           MTILQRTRSSSAAIKATTKLIPSSISGHRHHDFRSFSSSNLPPEYSNPNNFSGHHGHRHQ
Sbjct: 1   MTILQRTRSSSAAIKATTKLIPSSISGHRHHDFRSFSSSNLPPEYSNPNNFSGHHGHRHQ 60

Query: 61  TVTGSKFSILLHSLLKLLSVPTCKWLSIPTHLSVRPSLGRRVVTGTLFGRRRGHVTFAVQ 120
           TVTGSKFSILLHSLLKLLSVPTCKWLSIPTHLSVRPSLGRRVVTGTLFGRRRGHVTFAVQ
Sbjct: 61  TVTGSKFSILLHSLLKLLSVPTCKWLSIPTHLSVRPSLGRRVVTGTLFGRRRGHVTFAVQ 120

Query: 121 IHPQSEPVLLLELATSTSSLVKEMSSGLVRIALESEAPAYNDGRPKKLFEEPRWTMYCNG 180
           IHPQSEPVLLLELATSTSSLVKEMSSGLVRIALESEAPAYNDGRPKKLFEEPRWTMYCNG
Sbjct: 121 IHPQSEPVLLLELATSTSSLVKEMSSGLVRIALESEAPAYNDGRPKKLFEEPRWTMYCNG 180

Query: 181 RKCGHSMLRTCGEFDRYVLNMVQSVSVGAGVIPVVENGGKSSRSEGELLYMRAKFERVVG 240
           RKCGHSMLRTCGEFDRYVLNMVQSVSVGAGVIPVVENGGKSSRSEGELLYMRAKFERVVG
Sbjct: 181 RKCGHSMLRTCGEFDRYVLNMVQSVSVGAGVIPVVENGGKSSRSEGELLYMRAKFERVVG 240

Query: 241 SRDSEAFYMINPDSNGGPELSIFLLRI 268
           SRDSEAFYMINPDSNGGPELSIFLLRI
Sbjct: 241 SRDSEAFYMINPDSNGGPELSIFLLRI 267

BLAST of IVF0025385 vs. ExPASy TrEMBL
Match: A0A1S3CNY4 (protein MIZU-KUSSEI 1-like OS=Cucumis melo OX=3656 GN=LOC103502949 PE=4 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 1.9e-147
Identity = 267/267 (100.00%), Postives = 267/267 (100.00%), Query Frame = 0

Query: 1   MTILQRTRSSSAAIKATTKLIPSSISGHRHHDFRSFSSSNLPPEYSNPNNFSGHHGHRHQ 60
           MTILQRTRSSSAAIKATTKLIPSSISGHRHHDFRSFSSSNLPPEYSNPNNFSGHHGHRHQ
Sbjct: 1   MTILQRTRSSSAAIKATTKLIPSSISGHRHHDFRSFSSSNLPPEYSNPNNFSGHHGHRHQ 60

Query: 61  TVTGSKFSILLHSLLKLLSVPTCKWLSIPTHLSVRPSLGRRVVTGTLFGRRRGHVTFAVQ 120
           TVTGSKFSILLHSLLKLLSVPTCKWLSIPTHLSVRPSLGRRVVTGTLFGRRRGHVTFAVQ
Sbjct: 61  TVTGSKFSILLHSLLKLLSVPTCKWLSIPTHLSVRPSLGRRVVTGTLFGRRRGHVTFAVQ 120

Query: 121 IHPQSEPVLLLELATSTSSLVKEMSSGLVRIALESEAPAYNDGRPKKLFEEPRWTMYCNG 180
           IHPQSEPVLLLELATSTSSLVKEMSSGLVRIALESEAPAYNDGRPKKLFEEPRWTMYCNG
Sbjct: 121 IHPQSEPVLLLELATSTSSLVKEMSSGLVRIALESEAPAYNDGRPKKLFEEPRWTMYCNG 180

Query: 181 RKCGHSMLRTCGEFDRYVLNMVQSVSVGAGVIPVVENGGKSSRSEGELLYMRAKFERVVG 240
           RKCGHSMLRTCGEFDRYVLNMVQSVSVGAGVIPVVENGGKSSRSEGELLYMRAKFERVVG
Sbjct: 181 RKCGHSMLRTCGEFDRYVLNMVQSVSVGAGVIPVVENGGKSSRSEGELLYMRAKFERVVG 240

Query: 241 SRDSEAFYMINPDSNGGPELSIFLLRI 268
           SRDSEAFYMINPDSNGGPELSIFLLRI
Sbjct: 241 SRDSEAFYMINPDSNGGPELSIFLLRI 267

BLAST of IVF0025385 vs. ExPASy TrEMBL
Match: A0A6J1HPB2 (protein MIZU-KUSSEI 1-like OS=Cucurbita maxima OX=3661 GN=LOC111466084 PE=4 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 1.1e-97
Identity = 201/271 (74.17%), Postives = 219/271 (80.81%), Query Frame = 0

Query: 1   MTILQRTRSSSAAIKATTKLIPSSISGHRHHDFRSFSSSNLPPEYSNPNNFSGHHGHRHQ 60
           MT LQRT+S SAAIK TTK+IPSSISG  HH   S  + N   ++    N S H  HR  
Sbjct: 1   MTGLQRTKSCSAAIKTTTKIIPSSISG--HHLRSSSDADNFSAKFLLQKN-SSHRCHRQH 60

Query: 61  TV--TGSKFSILLHSLLKLLSVPTCKWLSIPTHLSVRPSLGRRVVTGTLFGRRRGHVTFA 120
               T  KFS LLHSLLKL+S+PTCKWLSIPTHLSV PSLGRR VTGTLFGRRRGHVTFA
Sbjct: 61  LAASTTGKFSSLLHSLLKLISIPTCKWLSIPTHLSVSPSLGRR-VTGTLFGRRRGHVTFA 120

Query: 121 VQIHPQSEPVLLLELATSTSSLVKEMSSGLVRIALESEAPAYNDGRP--KKLFEEPRWTM 180
           VQ+ P+SEPVLLLE ATSTSSL+KEMSSGLVRIALES+A    DG    +KL EEPRWTM
Sbjct: 121 VQLDPRSEPVLLLEFATSTSSLIKEMSSGLVRIALESDALTTYDGNRSIRKLLEEPRWTM 180

Query: 181 YCNGRKCGHSMLRTCGEFDRYVLNMVQSVSVGAGVIPVVENGGKSSRSEGELLYMRAKFE 240
           YCNGRKCG +M RTCGEF+ YVLN VQSVSVGAGVIP+V++G   SRSEGELLYMRAKFE
Sbjct: 181 YCNGRKCGQAMSRTCGEFEWYVLNTVQSVSVGAGVIPMVDDGHMGSRSEGELLYMRAKFE 240

Query: 241 RVVGSRDSEAFYMINPDSNGGPELSIFLLRI 268
           RVVGSRDSEAFYM+NPD NGGPELSIFLLRI
Sbjct: 241 RVVGSRDSEAFYMMNPDGNGGPELSIFLLRI 267

BLAST of IVF0025385 vs. ExPASy TrEMBL
Match: A0A6J1EAS0 (protein MIZU-KUSSEI 1 OS=Cucurbita moschata OX=3662 GN=LOC111432316 PE=4 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 2.4e-97
Identity = 200/271 (73.80%), Postives = 221/271 (81.55%), Query Frame = 0

Query: 1   MTILQRTRSSSAAIKATTKLIPSSISGHRHHDFRSFSSSNLPPEYSNPNNFSGHHGHRHQ 60
           MT LQRT+S SAAI+ TTK+IPSSIS   HH   S  + N   ++    N S H  HR  
Sbjct: 1   MTGLQRTKSCSAAIQTTTKIIPSSISA--HHLRSSSDADNFSGKFLLQKN-SSHRCHRQH 60

Query: 61  TV--TGSKFSILLHSLLKLLSVPTCKWLSIPTHLSVRPSLGRRVVTGTLFGRRRGHVTFA 120
               T  KFS LLHSLLKLLS+PTCKWLSIPTHLSV PSLGRR VTGTLFGRRRGHVTFA
Sbjct: 61  LAASTTGKFSSLLHSLLKLLSIPTCKWLSIPTHLSVSPSLGRR-VTGTLFGRRRGHVTFA 120

Query: 121 VQIHPQSEPVLLLELATSTSSLVKEMSSGLVRIALESEAPAYNDG-RP-KKLFEEPRWTM 180
           +Q+ P+S+PVLLLE ATSTSSL+KEMSSGLVRIALES+AP   DG RP +KL EEPRWTM
Sbjct: 121 IQLDPRSQPVLLLEFATSTSSLIKEMSSGLVRIALESDAPTTFDGNRPIRKLLEEPRWTM 180

Query: 181 YCNGRKCGHSMLRTCGEFDRYVLNMVQSVSVGAGVIPVVENGGKSSRSEGELLYMRAKFE 240
           YCNGRKCG +M RTCGEF+ YVLN VQSVSVGAGVIP+V++G K S SEGELLYMRAKFE
Sbjct: 181 YCNGRKCGQAMSRTCGEFEWYVLNTVQSVSVGAGVIPMVDDGRKGSGSEGELLYMRAKFE 240

Query: 241 RVVGSRDSEAFYMINPDSNGGPELSIFLLRI 268
           RV+GSRDSEAFYM+NPD NGGPELSIFLLRI
Sbjct: 241 RVIGSRDSEAFYMMNPDGNGGPELSIFLLRI 267

BLAST of IVF0025385 vs. ExPASy TrEMBL
Match: A0A6J1DFH1 (protein MIZU-KUSSEI 1 OS=Momordica charantia OX=3673 GN=LOC111020015 PE=4 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 6.8e-92
Identity = 191/271 (70.48%), Postives = 213/271 (78.60%), Query Frame = 0

Query: 1   MTILQRTRSSSAAIKATTKLIP-SSISGHRHHDFRSFSSSNLPPEYSNPNNFSGHHGHRH 60
           M+ LQRTRS SAAIK TTK+ P +S+SGH HH  R   SS+    +S         G  H
Sbjct: 1   MSTLQRTRSCSAAIKTTTKITPAASLSGH-HHRHRHVRSSSSSDGFSGNLLLQKKSGDHH 60

Query: 61  QTVTGSKFSILLHSLLKLLSVPTCKWLSIPTHLSVRPSLGRRVVTGTLFGRRRGHVTFAV 120
               G KFS L HSLL+LLS+PTCKWLSIP HLS  PSL RR VTGTLFG RRGHVTFAV
Sbjct: 61  HHPAG-KFSSLFHSLLRLLSIPTCKWLSIPNHLSATPSL-RRKVTGTLFGHRRGHVTFAV 120

Query: 121 QIHPQSEPVLLLELATSTSSLVKEMSSGLVRIALESEAPAYNDGRPKKLFEEPRWTMYCN 180
           Q+ P+SEPV LLELA ST+SLVKEMSSGLVRIALESEAPA  DGR ++LFEEPRWTMYCN
Sbjct: 121 QLEPRSEPVFLLELAVSTASLVKEMSSGLVRIALESEAPANEDGR-RRLFEEPRWTMYCN 180

Query: 181 GRKCGHSMLRTCGEFDRYVLNMVQSVSVGAGVIPVVEN---GGKSSRSEGELLYMRAKFE 240
           GRKCGH+  RTCG+F+ +VLN VQSVSVGAGVIPVV++   GG    S+GELLYMRA+FE
Sbjct: 181 GRKCGHATSRTCGDFEWHVLNTVQSVSVGAGVIPVVDDGRKGGGGCGSDGELLYMRARFE 240

Query: 241 RVVGSRDSEAFYMINPDSNGGPELSIFLLRI 268
           RVVGS DSEAFYM+NPD NGGPELSIFLLRI
Sbjct: 241 RVVGSHDSEAFYMMNPDGNGGPELSIFLLRI 267

BLAST of IVF0025385 vs. NCBI nr
Match: XP_008465295.1 (PREDICTED: protein MIZU-KUSSEI 1-like [Cucumis melo] >KAA0033204.1 protein MIZU-KUSSEI 1-like [Cucumis melo var. makuwa] >TYK07504.1 protein MIZU-KUSSEI 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 529 bits (1363), Expect = 1.88e-189
Identity = 267/267 (100.00%), Postives = 267/267 (100.00%), Query Frame = 0

Query: 1   MTILQRTRSSSAAIKATTKLIPSSISGHRHHDFRSFSSSNLPPEYSNPNNFSGHHGHRHQ 60
           MTILQRTRSSSAAIKATTKLIPSSISGHRHHDFRSFSSSNLPPEYSNPNNFSGHHGHRHQ
Sbjct: 1   MTILQRTRSSSAAIKATTKLIPSSISGHRHHDFRSFSSSNLPPEYSNPNNFSGHHGHRHQ 60

Query: 61  TVTGSKFSILLHSLLKLLSVPTCKWLSIPTHLSVRPSLGRRVVTGTLFGRRRGHVTFAVQ 120
           TVTGSKFSILLHSLLKLLSVPTCKWLSIPTHLSVRPSLGRRVVTGTLFGRRRGHVTFAVQ
Sbjct: 61  TVTGSKFSILLHSLLKLLSVPTCKWLSIPTHLSVRPSLGRRVVTGTLFGRRRGHVTFAVQ 120

Query: 121 IHPQSEPVLLLELATSTSSLVKEMSSGLVRIALESEAPAYNDGRPKKLFEEPRWTMYCNG 180
           IHPQSEPVLLLELATSTSSLVKEMSSGLVRIALESEAPAYNDGRPKKLFEEPRWTMYCNG
Sbjct: 121 IHPQSEPVLLLELATSTSSLVKEMSSGLVRIALESEAPAYNDGRPKKLFEEPRWTMYCNG 180

Query: 181 RKCGHSMLRTCGEFDRYVLNMVQSVSVGAGVIPVVENGGKSSRSEGELLYMRAKFERVVG 240
           RKCGHSMLRTCGEFDRYVLNMVQSVSVGAGVIPVVENGGKSSRSEGELLYMRAKFERVVG
Sbjct: 181 RKCGHSMLRTCGEFDRYVLNMVQSVSVGAGVIPVVENGGKSSRSEGELLYMRAKFERVVG 240

Query: 241 SRDSEAFYMINPDSNGGPELSIFLLRI 267
           SRDSEAFYMINPDSNGGPELSIFLLRI
Sbjct: 241 SRDSEAFYMINPDSNGGPELSIFLLRI 267

BLAST of IVF0025385 vs. NCBI nr
Match: XP_038877466.1 (protein MIZU-KUSSEI 1-like [Benincasa hispida])

HSP 1 Score: 441 bits (1134), Expect = 2.22e-154
Identity = 229/278 (82.37%), Postives = 240/278 (86.33%), Query Frame = 0

Query: 1   MTILQRTRSSSAAIKATTKLIPSSISGH--RHHDFRSFSSSNLPPEYSNPNNFSG----- 60
           MT LQRTRS  AAIK +TKLIPSSIS H  RHH  RSFSSSNLPPEYSNP+NFSG     
Sbjct: 1   MTGLQRTRSCMAAIKTSTKLIPSSISDHQHRHHHLRSFSSSNLPPEYSNPDNFSGRFLLH 60

Query: 61  ----HHGHRHQTVTGSKFSILLHSLLKLLSVPTCKWLSIPTHLSVRPSLGRRVVTGTLFG 120
               HHGH HQ   GSKFS LLHSLLKLLSVPTCKW SIPTHLSV  SLGRRVVTGTLFG
Sbjct: 61  KNSGHHGHHHQAAAGSKFSTLLHSLLKLLSVPTCKWFSIPTHLSVSSSLGRRVVTGTLFG 120

Query: 121 RRRGHVTFAVQIHPQSEPVLLLELATSTSSLVKEMSSGLVRIALESEAPAYNDGRPKKLF 180
           RRRGHVTFAVQ+ P+SEPVLLLELATSTS LVKEMSSGLVRIALESEAPAYN+ RP+KLF
Sbjct: 121 RRRGHVTFAVQLDPRSEPVLLLELATSTSFLVKEMSSGLVRIALESEAPAYNESRPRKLF 180

Query: 181 EEPRWTMYCNGRKCGHSMLRTCGEFDRYVLNMVQSVSVGAGVIPVVENGGKSSRSEGELL 240
           EEPRWTMYCNGRKCGHS LR CGEF+ YVLN VQ VSVGAGVIPVV++G K SRSEGELL
Sbjct: 181 EEPRWTMYCNGRKCGHSTLRVCGEFEWYVLNTVQRVSVGAGVIPVVDDGRKGSRSEGELL 240

Query: 241 YMRAKFERVVGSRDSEAFYMINPDSNGGPELSIFLLRI 267
           YMRA +ERVVGSRDSEAFYM+NPD NGGPELSIFLLRI
Sbjct: 241 YMRANYERVVGSRDSEAFYMMNPDGNGGPELSIFLLRI 278

BLAST of IVF0025385 vs. NCBI nr
Match: KAG7027634.1 (Protein MIZU-KUSSEI 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 365 bits (937), Expect = 1.46e-124
Identity = 201/271 (74.17%), Postives = 221/271 (81.55%), Query Frame = 0

Query: 1   MTILQRTRSSSAAIKATTKLIPSSISGHRHHDFRSFSSSNLPPEYSNPNNFSGHHGHRHQ 60
           MT LQRT+S SAAIK TTK+IPSSIS H  H   S  + N   ++    N S H  HR  
Sbjct: 1   MTGLQRTKSCSAAIKTTTKIIPSSISAH--HLRSSSDADNFSGKFLLQKN-SSHRCHRQH 60

Query: 61  TV--TGSKFSILLHSLLKLLSVPTCKWLSIPTHLSVRPSLGRRVVTGTLFGRRRGHVTFA 120
               T  KFS LLHSLLKLLS+PTCKWLSIPTHLSV PSLGRRV TGTLFGRRRGHVTFA
Sbjct: 61  LAASTTGKFSFLLHSLLKLLSIPTCKWLSIPTHLSVSPSLGRRV-TGTLFGRRRGHVTFA 120

Query: 121 VQIHPQSEPVLLLELATSTSSLVKEMSSGLVRIALESEAPAYNDG-RP-KKLFEEPRWTM 180
           +Q+ P+S+PVLLLE ATSTSSL+KEMSSGLVRIALES+AP   DG RP +KL EEPRWTM
Sbjct: 121 IQLDPRSQPVLLLEFATSTSSLIKEMSSGLVRIALESDAPTTFDGNRPIRKLLEEPRWTM 180

Query: 181 YCNGRKCGHSMLRTCGEFDRYVLNMVQSVSVGAGVIPVVENGGKSSRSEGELLYMRAKFE 240
           YCNGRKCG +M RTCGEF+ YVLN VQSVSVGAGVIP+V++G K S SEGELLYMRAKFE
Sbjct: 181 YCNGRKCGQAMSRTCGEFEWYVLNTVQSVSVGAGVIPMVDDGRKGSGSEGELLYMRAKFE 240

Query: 241 RVVGSRDSEAFYMINPDSNGGPELSIFLLRI 267
           RV+GSRDSEAFYM+NPD NGGPELSIFLLRI
Sbjct: 241 RVIGSRDSEAFYMMNPDGNGGPELSIFLLRI 267

BLAST of IVF0025385 vs. NCBI nr
Match: XP_022966431.1 (protein MIZU-KUSSEI 1-like [Cucurbita maxima])

HSP 1 Score: 364 bits (934), Expect = 4.19e-124
Identity = 201/271 (74.17%), Postives = 219/271 (80.81%), Query Frame = 0

Query: 1   MTILQRTRSSSAAIKATTKLIPSSISGHRHHDFRSFSSSNLPPEYSNPNNFSGHHGHRHQ 60
           MT LQRT+S SAAIK TTK+IPSSISGH  H   S  + N   ++    N S H  HR  
Sbjct: 1   MTGLQRTKSCSAAIKTTTKIIPSSISGH--HLRSSSDADNFSAKFLLQKN-SSHRCHRQH 60

Query: 61  TV--TGSKFSILLHSLLKLLSVPTCKWLSIPTHLSVRPSLGRRVVTGTLFGRRRGHVTFA 120
               T  KFS LLHSLLKL+S+PTCKWLSIPTHLSV PSLGRRV TGTLFGRRRGHVTFA
Sbjct: 61  LAASTTGKFSSLLHSLLKLISIPTCKWLSIPTHLSVSPSLGRRV-TGTLFGRRRGHVTFA 120

Query: 121 VQIHPQSEPVLLLELATSTSSLVKEMSSGLVRIALESEAPAYNDGRP--KKLFEEPRWTM 180
           VQ+ P+SEPVLLLE ATSTSSL+KEMSSGLVRIALES+A    DG    +KL EEPRWTM
Sbjct: 121 VQLDPRSEPVLLLEFATSTSSLIKEMSSGLVRIALESDALTTYDGNRSIRKLLEEPRWTM 180

Query: 181 YCNGRKCGHSMLRTCGEFDRYVLNMVQSVSVGAGVIPVVENGGKSSRSEGELLYMRAKFE 240
           YCNGRKCG +M RTCGEF+ YVLN VQSVSVGAGVIP+V++G   SRSEGELLYMRAKFE
Sbjct: 181 YCNGRKCGQAMSRTCGEFEWYVLNTVQSVSVGAGVIPMVDDGHMGSRSEGELLYMRAKFE 240

Query: 241 RVVGSRDSEAFYMINPDSNGGPELSIFLLRI 267
           RVVGSRDSEAFYM+NPD NGGPELSIFLLRI
Sbjct: 241 RVVGSRDSEAFYMMNPDGNGGPELSIFLLRI 267

BLAST of IVF0025385 vs. NCBI nr
Match: XP_022924911.1 (protein MIZU-KUSSEI 1 [Cucurbita moschata])

HSP 1 Score: 363 bits (931), Expect = 1.20e-123
Identity = 200/271 (73.80%), Postives = 221/271 (81.55%), Query Frame = 0

Query: 1   MTILQRTRSSSAAIKATTKLIPSSISGHRHHDFRSFSSSNLPPEYSNPNNFSGHHGHRHQ 60
           MT LQRT+S SAAI+ TTK+IPSSIS H  H   S  + N   ++    N S H  HR  
Sbjct: 1   MTGLQRTKSCSAAIQTTTKIIPSSISAH--HLRSSSDADNFSGKFLLQKN-SSHRCHRQH 60

Query: 61  TV--TGSKFSILLHSLLKLLSVPTCKWLSIPTHLSVRPSLGRRVVTGTLFGRRRGHVTFA 120
               T  KFS LLHSLLKLLS+PTCKWLSIPTHLSV PSLGRRV TGTLFGRRRGHVTFA
Sbjct: 61  LAASTTGKFSSLLHSLLKLLSIPTCKWLSIPTHLSVSPSLGRRV-TGTLFGRRRGHVTFA 120

Query: 121 VQIHPQSEPVLLLELATSTSSLVKEMSSGLVRIALESEAPAYNDG-RP-KKLFEEPRWTM 180
           +Q+ P+S+PVLLLE ATSTSSL+KEMSSGLVRIALES+AP   DG RP +KL EEPRWTM
Sbjct: 121 IQLDPRSQPVLLLEFATSTSSLIKEMSSGLVRIALESDAPTTFDGNRPIRKLLEEPRWTM 180

Query: 181 YCNGRKCGHSMLRTCGEFDRYVLNMVQSVSVGAGVIPVVENGGKSSRSEGELLYMRAKFE 240
           YCNGRKCG +M RTCGEF+ YVLN VQSVSVGAGVIP+V++G K S SEGELLYMRAKFE
Sbjct: 181 YCNGRKCGQAMSRTCGEFEWYVLNTVQSVSVGAGVIPMVDDGRKGSGSEGELLYMRAKFE 240

Query: 241 RVVGSRDSEAFYMINPDSNGGPELSIFLLRI 267
           RV+GSRDSEAFYM+NPD NGGPELSIFLLRI
Sbjct: 241 RVIGSRDSEAFYMMNPDGNGGPELSIFLLRI 267

BLAST of IVF0025385 vs. TAIR 10
Match: AT2G41660.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 226.5 bits (576), Expect = 2.6e-59
Identity = 146/282 (51.77%), Postives = 182/282 (64.54%), Query Frame = 0

Query: 8   RSSSAAIKATTKLIPSSISGHRHHDFRSFSSSNLPPEYSNPNNFSGHHGHRHQTVTGSKF 67
           RSS     +++ LIP SI  H          S +P   S+ ++ S + G  H      KF
Sbjct: 28  RSSHVRSPSSSALIP-SIPEHELFLVPCRRCSYVP--LSSSSSASHNIGKFH-----LKF 87

Query: 68  SILLHSLLKLLSVPTCKWLSIPTHLSV---------------RPSLGRRVVTGTLFGRRR 127
           S+LL S + ++++P CK LS+P+  S                  SLGRR VTGTL+G +R
Sbjct: 88  SLLLRSFINIINIPACKMLSLPSPPSSSSSVSNQLISLVTGGSSSLGRR-VTGTLYGHKR 147

Query: 128 GHVTFAVQIHPQSEPVLLLELATSTSSLVKEMSSGLVRIALESEAPAYNDGRPKKLFEEP 187
           GHVTF+VQ + +S+PVLLL+LA ST++LVKEMSSGLVRIALE E   +  G   KLF+EP
Sbjct: 148 GHVTFSVQYNQRSDPVLLLDLAMSTATLVKEMSSGLVRIALECE-KRHRSG--TKLFQEP 207

Query: 188 RWTMYCNGRKCGHSMLR--TCGEFDRYVLNMVQSVSVGAGVIPVVE-----NGGKSSRSE 247
           +WTMYCNGRKCG+++ R   C + D  VLN V  V+VGAGVIP  +     +G  S    
Sbjct: 208 KWTMYCNGRKCGYAVSRGGACTDTDWRVLNTVSRVTVGAGVIPTPKTIDDVSGVGSGTEL 267

Query: 248 GELLYMRAKFERVVGSRDSEAFYMINPDSNGGPELSIFLLRI 268
           GELLYMR KFERVVGSRDSEAFYM+NPD NGGPELSIFLLRI
Sbjct: 268 GELLYMRGKFERVVGSRDSEAFYMMNPDKNGGPELSIFLLRI 297

BLAST of IVF0025385 vs. TAIR 10
Match: AT5G23100.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 193.0 bits (489), Expect = 3.1e-49
Identity = 115/243 (47.33%), Postives = 144/243 (59.26%), Query Frame = 0

Query: 54  HHGHRHQTVTGSKFSILLHSLLKLLSVPTCKWLSIPTHLS-VRP-----SLGRRVVTGTL 113
           H+  +  + + S  S ++    KL S    +  S+   LS  RP      LG RVV GTL
Sbjct: 36  HNNTKPSSSSSSSSSNVITHKKKLQSFAVSRLRSVIATLSRARPGNQNSGLGSRVV-GTL 95

Query: 114 FGRRRGHVTFAVQIHPQSEPVLLLELATSTSSLVKEMSSGLVRIALE-SEAPAYNDGRPK 173
           FG RRGHV F++Q  P S P  L+ELAT  S LVKEM+SGLVRIALE  +     +G  K
Sbjct: 96  FGSRRGHVHFSIQKDPNSPPAFLIELATPISGLVKEMASGLVRIALECDKGKEEEEGEEK 155

Query: 174 ----------------------KLFEEPRWTMYCNGRKCGHSMLRTCGEFDRYVLNMVQS 233
                                 +L EEP W  YCNG+KCG +  R CGE ++ VL  ++ 
Sbjct: 156 NGTLRHGGGDKTKTTTTAAVSRRLVEEPMWRTYCNGKKCGFATRRECGEKEKKVLKALEM 215

Query: 234 VSVGAGVIPVVENGGKSSRSEGELLYMRAKFERVVGSRDSEAFYMINPDSNGGPELSIFL 268
           VS+GAGV+P  E  G      G+++YMRAKFER+VGSRDSEAFYM+NPDSNG PELSI+L
Sbjct: 216 VSMGAGVLPETEEIGGGGGGGGDIMYMRAKFERIVGSRDSEAFYMMNPDSNGAPELSIYL 275

BLAST of IVF0025385 vs. TAIR 10
Match: AT3G25640.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 188.0 bits (476), Expect = 1.0e-47
Identity = 101/177 (57.06%), Postives = 124/177 (70.06%), Query Frame = 0

Query: 98  LGRRVVTGTLFGRRRGHVTFAVQIHPQSEPVLLLELATSTSSLVKEMSSGLVRIALESEA 157
           LG RVV GTLFG RRGHV FAVQ  P   P +L++L T TS LV+EM+SGLVRIALE+  
Sbjct: 94  LGFRVV-GTLFGNRRGHVYFAVQDDPTRLPAVLIQLPTPTSVLVREMASGLVRIALET-- 153

Query: 158 PAYNDGRPKKLFEEPRWTMYCNGRKCGHSMLRTCGEFDRYVLNMVQSVSVGAGVIPVV-- 217
            AY     KKL EE  W  YCNG+KCG++  + CGE +  VL  V  +++GAGV+P    
Sbjct: 154 AAYKTDSKKKLLEESTWRTYCNGKKCGYAARKECGEAEWKVLKAVGPITMGAGVLPATTT 213

Query: 218 ----ENGGKSSRSEGELLYMRAKFERVVGSRDSEAFYMINPD-SNGGPELSIFLLRI 268
               E  G     +GEL+YMRA+FERVVGSRDSEAFYM+NPD S+GGPELS++ LR+
Sbjct: 214 TVDEEGNGAVGSEKGELMYMRARFERVVGSRDSEAFYMMNPDVSSGGPELSVYFLRV 267

BLAST of IVF0025385 vs. TAIR 10
Match: AT5G06990.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 170.2 bits (430), Expect = 2.2e-42
Identity = 100/218 (45.87%), Postives = 133/218 (61.01%), Query Frame = 0

Query: 63  TGSKFSIL---LHSLLKLLSV--PTCKW-LSIPTHLSVRPSLGRRVVTGTLFGRRRGHVT 122
           TGSK + L   + S+ + L +  P CK+ +     L      G   VTGTLFG R+  V 
Sbjct: 47  TGSKSTKLFRRVRSVFRSLPIMSPMCKFPVGGGGRLHENHVHGGTRVTGTLFGYRKTRVN 106

Query: 123 FAVQIHPQSEPVLLLELATSTSSLVKEMSSGLVRIALESEAPAYNDGRPKKLFEEPRWTM 182
            AVQ +P+S P+LLLELA  T  L++++  GLVRIALE E          K+ +EP W +
Sbjct: 107 LAVQENPRSLPILLLELAIPTGKLLQDLGVGLVRIALECEK---KPSEKTKIIDEPIWAL 166

Query: 183 YCNGRKCGHSMLRTCGEFDRYVLNMVQSVSVGAGVIPV-------VENGGKSSRSEGELL 242
           YCNG+K G+ + R   E D  V+ M+ +VS+GAGV+PV          GG   + EG+L 
Sbjct: 167 YCNGKKSGYGVKRQPTEEDLVVMQMLHAVSMGAGVLPVSSGAITEQSGGGGGGQQEGDLT 226

Query: 243 YMRAKFERVVGSRDSEAFYMINPDSNGGPELSIFLLRI 268
           YMRA FERV+GSRDSE +YM+NPD N GPELSIF +R+
Sbjct: 227 YMRAHFERVIGSRDSETYYMMNPDGNSGPELSIFFVRV 261

BLAST of IVF0025385 vs. TAIR 10
Match: AT4G39610.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 163.3 bits (412), Expect = 2.7e-40
Identity = 81/171 (47.37%), Postives = 115/171 (67.25%), Query Frame = 0

Query: 103 VTGTLFGRRRGHVTFAVQIHPQSEPVLLLELATSTSSLVKEMSSGLVRIALESEAPAYND 162
           +TGTLFG R+G V+ ++Q +P+  P L++ELA  T++L KE+S+G+VRIALE+E     D
Sbjct: 97  ITGTLFGYRKGRVSLSIQENPKCLPSLVVELAMQTTTLQKELSTGMVRIALETEKQPRAD 156

Query: 163 GRPKK------LFEEPRWTMYCNGRKCGHSMLRTCGEFDRYVLNMVQSVSVGAGVIPVVE 222
               K      + EEP WTMYC G K G+ + R   E D  V+ +++ VS+GAGV+P   
Sbjct: 157 NNNSKTEKKTDILEEPLWTMYCKGEKTGYGVKREATEEDLNVMELLRPVSMGAGVLP--- 216

Query: 223 NGGKSSRSEGELLYMRAKFERVVGSRDSEAFYMINPDSNGGPELSIFLLRI 268
              +S   +GE+ YMRA FERV+GS+DSE FYM++P+ N GPELS F +R+
Sbjct: 217 GNSESEGPDGEMAYMRAYFERVIGSKDSETFYMLSPEGNNGPELSFFFVRV 264

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O222273.6e-5851.77Protein MIZU-KUSSEI 1 OS=Arabidopsis thaliana OX=3702 GN=MIZ1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7SPQ91.9e-147100.00Protein MIZU-KUSSEI 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A1S3CNY41.9e-147100.00protein MIZU-KUSSEI 1-like OS=Cucumis melo OX=3656 GN=LOC103502949 PE=4 SV=1[more]
A0A6J1HPB21.1e-9774.17protein MIZU-KUSSEI 1-like OS=Cucurbita maxima OX=3661 GN=LOC111466084 PE=4 SV=1[more]
A0A6J1EAS02.4e-9773.80protein MIZU-KUSSEI 1 OS=Cucurbita moschata OX=3662 GN=LOC111432316 PE=4 SV=1[more]
A0A6J1DFH16.8e-9270.48protein MIZU-KUSSEI 1 OS=Momordica charantia OX=3673 GN=LOC111020015 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_008465295.11.88e-189100.00PREDICTED: protein MIZU-KUSSEI 1-like [Cucumis melo] >KAA0033204.1 protein MIZU-... [more]
XP_038877466.12.22e-15482.37protein MIZU-KUSSEI 1-like [Benincasa hispida][more]
KAG7027634.11.46e-12474.17Protein MIZU-KUSSEI 1, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022966431.14.19e-12474.17protein MIZU-KUSSEI 1-like [Cucurbita maxima][more]
XP_022924911.11.20e-12373.80protein MIZU-KUSSEI 1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT2G41660.12.6e-5951.77Protein of unknown function, DUF617 [more]
AT5G23100.13.1e-4947.33Protein of unknown function, DUF617 [more]
AT3G25640.11.0e-4757.06Protein of unknown function, DUF617 [more]
AT5G06990.12.2e-4245.87Protein of unknown function, DUF617 [more]
AT4G39610.12.7e-4047.37Protein of unknown function, DUF617 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006460Protein MIZU-KUSSEI 1-like, plantPFAMPF04759DUF617coord: 103..266
e-value: 8.8E-66
score: 221.0
IPR006460Protein MIZU-KUSSEI 1-like, plantTIGRFAMTIGR01570TIGR01570coord: 103..267
e-value: 2.2E-67
score: 224.0
IPR006460Protein MIZU-KUSSEI 1-like, plantPANTHERPTHR31696PROTEIN MIZU-KUSSEI 1coord: 14..267
NoneNo IPR availablePANTHERPTHR31696:SF71PROTEIN MIZU-KUSSEI 1coord: 14..267

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0025385.2IVF0025385.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009658 chloroplast organization
biological_process GO:0010274 hydrotropism
cellular_component GO:0032541 cortical endoplasmic reticulum
cellular_component GO:0005789 endoplasmic reticulum membrane