IVF0023421 (gene) Melon (IVF77) v1

Overview


Name	IVF0023421
Type	gene
Organism	Cucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Description	Cysteine protease
Location	chr01: 492618 .. 495016 (+)
RNA-Seq Expression	IVF0023421
Synteny	IVF0023421

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGATTTCTTCACCCATTTTCTTCGCTTTTCTTGCCCTTTTCCTCTGTCTTTCCCCTTTTTCATCCGCTTCCCATTCATCCACCTTCTCCATCATTGATGAAAACGCTAAACACCACTTGGGTATCCCTGAAATTCCCCATTCCGATGCCCATCAACGCACCGACGAGGAAGTTGCCGCTTTGTACGAGTCCTGGTTGGTCCATCATGGAAAAGCCTACAATGCTCTTGGTGAGAAGGAGAGACGGTTTGAGATTTTTAAGGATAATCTCATGTTTATTGATGAACATAACCGGGAATCGCGGACGTATAAAGTTGGATTAACCCGTTTTGCTGATCTTACCAATGAGGAATATCGGGCAAGGTTTTGGGTGGCCGATTCTCCCGGAAGCCTAGCTTATCCGCCGCCAAGAGCGGAAGGTACGCGGCGGCGCTCGGTGATGATCTTCCGGATGATGTCGATTGGAGAAAAAAGGGCGCTGTTGCTAATGTTAAAGATCAAGGACAGTGTGGTGAGTTTTCTTTTTATTTTTTCTCTCTCTTTTTTTAAACTTTTAATTTTTTTTGTTGAATTGATCCCATGAGAATGATGCTTGGATTTTGTGTTTTCTTTTCGTTTTCTCATTTGGTCTTTGATATTTTGACTATTGTGTTTGGGTCGTTGCTGATTTTCCCAGGAAATTTGTTATGAGATCCATATTTATAGGTAACATTTCTCACAAATAGATTCATTAGATATTTGTCAATACTCAACCTTGATCGTAACTTGTATGTGTTATAATTAGCTTCGCTTCATCTCTGATGATTTGTTGATGGCCATCGATAACATCAGAAAACGTATTAGAAACTTTCCGCTCAAAACTTGTTTTCCAAGTAACCTTTTATCTCGAAGGATTGTTTTAGTAATGGCTTTTGATGGATGGCGATTGACGTAATTGGGTTATTTTTGGAATGAAGGGAGCTGTTGGGCTTTCTCAACAGTTGCTGCAGTGGAAGGAATAAACCAAATTGTCACTGGCGAATTGATCTCTCTGTCAGAGCAGGAACTTGTGGATTGTGACAAATCATTTAACATGGGTTGCAATGGTGGTCTTATGGACTATGCTTTCCAGTTCATCATTGACAATGGTGGAATTGACACCGACGAAGATTACCCTTACAAGGGACGAGATGGCGCTTGCGATCCCAATCGGGTTTGTCCCCAATCAACTTCACTTGATTCATTTATCTTACCATTTATCGTAATTTGACTGGTTTTTAATGTTTTGTCCAAAGAAAAATGCCAAGGTTGTAACCATAGATGGATATGAAGATGTTCCTGAGAATGATGAGAGCTCCTTGAAAAAGGCTGTAGCAAATCAACCAGTTAGTGTTGCAATTGAAGCTGGTGGCAGAGCCTTCCAACTTTACCAATCGGTAATCTCTTCAACTTTGAGCAATTTCCTGAATGTTGTTTCACAAAGCTAAGGTTTTGACTTCTCTGGCTTTGTAATGGTGGTATCTGTTTTGGAAGCTTATTCGAACATATTTTGTTGTGTGTTAATTCATCATTCTGGTTTGGGAATCAATATTCGAGTTTAATCTGTTGTGTTTGCGTGTCTTAATGACAAAGATAATCTGTATTGATCCACCATTTAGGCATGGGAAATGTTAGCTATTTCAAACACTTTGTCCTTATGCATCATATAAGGTGTTTTGTGTGTTTTAAATACAATATGTGCTTATGCATCAATTCAGGGTGTCTTCACTGGCCGTTGCGGAACCAATCTAGATCATGGTGTTGTTGCTGTTGGATATGGAACCGACAACGGTACAGATTATTGGATTGTGAGGAATTCATGGGGTAAAGACTGGGGAGAGAATGGTTATATCAGGTTGGAGAGAAATGTGGCCAACAGTACCACAGGCAAGTGTGGTATTGCAGTAGAGCCTTCATACCCCATCAAATCCGGATCAAACCCTCCAAAACCTTCTGCTTCTCCCCCATCTCCTGTCAACCCTCCAACTGAATGCGACGAATACTTCTCGTGCGATGAAGGAAGTACCTGCTGCTGTATCTATCAGTATGGAAGCACCTGCTTTGCTTGGGGATGCTGCCCCCTCGAGTCCGCAACCTGCTGTGATGACCATTACAGTTGCTGCCCACATGAATATCCCGTTTGTGACCTTGAGGCAGGAACTTGCCGAGCGGTACGTCACATCACATAGTTTTCTAACCAATGCTCATTCTTCAACGAGTTCTTTGTCATTCAAGTTCATGTTTGATATAATCTAATCATTGTAAATGGATGAAACACAGAGCAAAGACAGCTTGATGGGCGTCAATCTGCTGAAGCGCCTTCCTGCAAATCAGACAAAGCGGATTCAAAAGCTTGGGAAGTTGTTCGTTGGTGCTTGA

mRNA sequence

ATGGCGATTTCTTCACCCATTTTCTTCGCTTTTCTTGCCCTTTTCCTCTGTCTTTCCCCTTTTTCATCCGCTTCCCATTCATCCACCTTCTCCATCATTGATGAAAACGCTAAACACCACTTGGGTATCCCTGAAATTCCCCATTCCGATGCCCATCAACGCACCGACGAGGAAGTTGCCGCTTTGTACGAGTCCTGGTTGGTCCATCATGGAAAAGCCTACAATGCTCTTGGTGAGAAGGAGAGACGGTTTGAGATTTTTAAGGATAATCTCATGTTTATTGATGAACATAACCGGGAATCGCGGACGTATAAAGTTGGATTAACCCGTTTTGCTGATCTTACCAATGAGGAATATCGGGCAAGCTTATCCGCCGCCAAGAGCGGAAGGTACGCGGCGGCGCTCGGTGATGATCTTCCGGATGATGTCGATTGGAGAAAAAAGGGCGCTGTTGCTAATGTTAAAGATCAAGGACAGTGTGGGAGCTGTTGGGCTTTCTCAACAGTTGCTGCAGTGGAAGGAATAAACCAAATTGTCACTGGCGAATTGATCTCTCTGTCAGAGCAGGAACTTGTGGATTGTGACAAATCATTTAACATGGGTTGCAATGGTGGTCTTATGGACTATGCTTTCCAGTTCATCATTGACAATGGTGGAATTGACACCGACGAAGATTACCCTTACAAGGGACGAGATGGCGCTTGCGATCCCAATCGGAAAAATGCCAAGGTTGTAACCATAGATGGATATGAAGATGTTCCTGAGAATGATGAGAGCTCCTTGAAAAAGGCTGTAGCAAATCAACCAGTTAGTGTTGCAATTGAAGCTGGTGGCAGAGCCTTCCAACTTTACCAATCGGGTGTCTTCACTGGCCGTTGCGGAACCAATCTAGATCATGGTGTTGTTGCTGTTGGATATGGAACCGACAACGGTACAGATTATTGGATTGTGAGGAATTCATGGGGTAAAGACTGGGGAGAGAATGGTTATATCAGGTTGGAGAGAAATGTGGCCAACAGTACCACAGGCAAGTGTGGTATTGCAGTAGAGCCTTCATACCCCATCAAATCCGGATCAAACCCTCCAAAACCTTCTGCTTCTCCCCCATCTCCTGTCAACCCTCCAACTGAATGCGACGAATACTTCTCGTGCGATGAAGGAAGTACCTGCTGCTGTATCTATCAGTATGGAAGCACCTGCTTTGCTTGGGGATGCTGCCCCCTCGAGTCCGCAACCTGCTGTGATGACCATTACAGTTGCTGCCCACATGAATATCCCGTTTGTGACCTTGAGGCAGGAACTTGCCGAGCGAGCAAAGACAGCTTGATGGGCGTCAATCTGCTGAAGCGCCTTCCTGCAAATCAGACAAAGCGGATTCAAAAGCTTGGGAAGTTGTTCGTTGGTGCTTGA

Coding sequence (CDS)

ATGGCGATTTCTTCACCCATTTTCTTCGCTTTTCTTGCCCTTTTCCTCTGTCTTTCCCCTTTTTCATCCGCTTCCCATTCATCCACCTTCTCCATCATTGATGAAAACGCTAAACACCACTTGGGTATCCCTGAAATTCCCCATTCCGATGCCCATCAACGCACCGACGAGGAAGTTGCCGCTTTGTACGAGTCCTGGTTGGTCCATCATGGAAAAGCCTACAATGCTCTTGGTGAGAAGGAGAGACGGTTTGAGATTTTTAAGGATAATCTCATGTTTATTGATGAACATAACCGGGAATCGCGGACGTATAAAGTTGGATTAACCCGTTTTGCTGATCTTACCAATGAGGAATATCGGGCAAGCTTATCCGCCGCCAAGAGCGGAAGGTACGCGGCGGCGCTCGGTGATGATCTTCCGGATGATGTCGATTGGAGAAAAAAGGGCGCTGTTGCTAATGTTAAAGATCAAGGACAGTGTGGGAGCTGTTGGGCTTTCTCAACAGTTGCTGCAGTGGAAGGAATAAACCAAATTGTCACTGGCGAATTGATCTCTCTGTCAGAGCAGGAACTTGTGGATTGTGACAAATCATTTAACATGGGTTGCAATGGTGGTCTTATGGACTATGCTTTCCAGTTCATCATTGACAATGGTGGAATTGACACCGACGAAGATTACCCTTACAAGGGACGAGATGGCGCTTGCGATCCCAATCGGAAAAATGCCAAGGTTGTAACCATAGATGGATATGAAGATGTTCCTGAGAATGATGAGAGCTCCTTGAAAAAGGCTGTAGCAAATCAACCAGTTAGTGTTGCAATTGAAGCTGGTGGCAGAGCCTTCCAACTTTACCAATCGGGTGTCTTCACTGGCCGTTGCGGAACCAATCTAGATCATGGTGTTGTTGCTGTTGGATATGGAACCGACAACGGTACAGATTATTGGATTGTGAGGAATTCATGGGGTAAAGACTGGGGAGAGAATGGTTATATCAGGTTGGAGAGAAATGTGGCCAACAGTACCACAGGCAAGTGTGGTATTGCAGTAGAGCCTTCATACCCCATCAAATCCGGATCAAACCCTCCAAAACCTTCTGCTTCTCCCCCATCTCCTGTCAACCCTCCAACTGAATGCGACGAATACTTCTCGTGCGATGAAGGAAGTACCTGCTGCTGTATCTATCAGTATGGAAGCACCTGCTTTGCTTGGGGATGCTGCCCCCTCGAGTCCGCAACCTGCTGTGATGACCATTACAGTTGCTGCCCACATGAATATCCCGTTTGTGACCTTGAGGCAGGAACTTGCCGAGCGAGCAAAGACAGCTTGATGGGCGTCAATCTGCTGAAGCGCCTTCCTGCAAATCAGACAAAGCGGATTCAAAAGCTTGGGAAGTTGTTCGTTGGTGCTTGA

Protein sequence

MAISSPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVAALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYRASLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQCGSCWAFSTVAAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGIDTDEDYPYKGRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVFTGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTTGKCGIAVEPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCFAWGCCPLESATCCDDHYSCCPHEYPVCDLEAGTCRASKDSLMGVNLLKRLPANQTKRIQKLGKLFVGA

Homology

[Close]

BLAST of IVF0023421 vs. ExPASy Swiss-Prot
Match: P43297 (Cysteine proteinase RD21A OS=Arabidopsis thaliana OX=3702 GN=RD21A PE=1 SV=1)

HSP 1 Score: 630.2 bits (1624), Expect = 1.9e-179
Identity = 304/451 (67.41%), Postives = 357/451 (79.16%), Query Frame = 0


Query: 14  LFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVAALYESWLVHHGKA 73
           LFL +   SSA      SII  + KH +       S    R++ EV ++YE+WLV HGKA
Sbjct: 11  LFLAMVAVSSA---VDMSIISYDEKHGV-------STTGGRSEAEVMSIYEAWLVKHGKA 70

Query: 74  --YNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYRASLSAAK---- 133
              N+L EK+RRFEIFKDNL F+DEHN ++ +Y++GLTRFADLTN+EYR+    AK    
Sbjct: 71  QSQNSLVEKDRRFEIFKDNLRFVDEHNEKNLSYRLGLTRFADLTNDEYRSKYLGAKMEKK 130

Query: 134 -----SGRYAAALGDDLPDDVDWRKKGAVANVKDQGQCGSCWAFSTVAAVEGINQIVTGE 193
                S RY A +GD+LP+ +DWRKKGAVA VKDQG CGSCWAFST+ AVEGINQIVTG+
Sbjct: 131 GERRTSLRYEARVGDELPESIDWRKKGAVAEVKDQGGCGSCWAFSTIGAVEGINQIVTGD 190

Query: 194 LISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGIDTDEDYPYKGRDGACDPNRKNA 253
           LI+LSEQELVDCD S+N GCNGGLMDYAF+FII NGGIDTD+DYPYKG DG CD  RKNA
Sbjct: 191 LITLSEQELVDCDTSYNEGCNGGLMDYAFEFIIKNGGIDTDKDYPYKGVDGTCDQIRKNA 250

Query: 254 KVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVFTGRCGTNLDHGVV 313
           KVVTID YEDVP   E SLKKAVA+QP+S+AIEAGGRAFQLY SG+F G CGT LDHGVV
Sbjct: 251 KVVTIDSYEDVPTYSEESLKKAVAHQPISIAIEAGGRAFQLYDSGIFDGSCGTQLDHGVV 310

Query: 314 AVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTTGKCGIAVEPSYPIKSGSNPP 373
           AVGYGT+NG DYWIVRNSWGK WGE+GY+R+ RN+A S++GKCGIA+EPSYPIK+G NPP
Sbjct: 311 AVGYGTENGKDYWIVRNSWGKSWGESGYLRMARNIA-SSSGKCGIAIEPSYPIKNGENPP 370

Query: 374 KPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCFAWGCCPLESATCCDDHYSCCP 433
            P  SPPSP+ PPT+CD Y++C E +TCCC+++YG  CFAWGCCPLE+ATCCDD+YSCCP
Sbjct: 371 NPGPSPPSPIKPPTQCDSYYTCPESNTCCCLFEYGKYCFAWGCCPLEAATCCDDNYSCCP 430

Query: 434 HEYPVCDLEAGTCRASKDSLMGVNLLKRLPA 454
           HEYPVCDL+ GTC  SK+S   V  LKR PA
Sbjct: 431 HEYPVCDLDQGTCLLSKNSPFSVKALKRKPA 450

[Close]

BLAST of IVF0023421 vs. ExPASy Swiss-Prot
Match: Q9FMH8 (Probable cysteine protease RD21B OS=Arabidopsis thaliana OX=3702 GN=RD21B PE=1 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 8.8e-177
Identity = 303/457 (66.30%), Postives = 350/457 (76.59%), Query Frame = 0


Query: 9   FAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVAALYESWLV 68
           F  L+  + L      S++   SII  +  HH+       +    R+D EV  +YE+W+V
Sbjct: 3   FLKLSPMILLLAMIGVSYAMDMSIISYDENHHI-------TTETSRSDSEVERIYEAWMV 62

Query: 69  HHGKA---YNALG-EKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYRASLS 128
            HGK     N LG EK++RFEIFKDNL FIDEHN ++ +YK+GLTRFADLTNEEYR+   
Sbjct: 63  EHGKKKMNQNGLGAEKDQRFEIFKDNLRFIDEHNTKNLSYKLGLTRFADLTNEEYRSMYL 122

Query: 129 AAK--------SGRYAAALGDDLPDDVDWRKKGAVANVKDQGQCGSCWAFSTVAAVEGIN 188
            AK        S RY A +GD LPD VDWRK+GAVA+VKDQG CGSCWAFST+ AVEGIN
Sbjct: 123 GAKPTKRVLKTSDRYQARVGDALPDSVDWRKEGAVADVKDQGSCGSCWAFSTIGAVEGIN 182

Query: 189 QIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGIDTDEDYPYKGRDGACD 248
           +IVTG+LISLSEQELVDCD S+N GCNGGLMDYAF+FII NGGIDT+ DYPYK  DG CD
Sbjct: 183 KIVTGDLISLSEQELVDCDTSYNQGCNGGLMDYAFEFIIKNGGIDTEADYPYKAADGRCD 242

Query: 249 PNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVFTGRCGTN 308
            NRKNAKVVTID YEDVPEN E+SLKKA+A+QP+SVAIEAGGRAFQLY SGVF G CGT 
Sbjct: 243 QNRKNAKVVTIDSYEDVPENSEASLKKALAHQPISVAIEAGGRAFQLYSSGVFDGLCGTE 302

Query: 309 LDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTTGKCGIAVEPSYPIK 368
           LDHGVVAVGYGT+NG DYWIVRNSWG  WGE+GYI++ RN+  + TGKCGIA+E SYPIK
Sbjct: 303 LDHGVVAVGYGTENGKDYWIVRNSWGNRWGESGYIKMARNI-EAPTGKCGIAMEASYPIK 362

Query: 369 SGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCFAWGCCPLESATCCDD 428
            G NPP P  SPPSP+ PPT CD+YFSC E +TCCC+Y+YG  CF WGCCPLE+ATCCDD
Sbjct: 363 KGQNPPNPGPSPPSPIKPPTTCDKYFSCPESNTCCCLYKYGKYCFGWGCCPLEAATCCDD 422

Query: 429 HYSCCPHEYPVCDLEAGTCRASKDSLMGVNLLKRLPA 454
           + SCCPHEYPVCD+  GTC  SK+S   V  LKR PA
Sbjct: 423 NSSCCPHEYPVCDVNRGTCLMSKNSPFSVKALKRTPA 451

[Close]

BLAST of IVF0023421 vs. ExPASy Swiss-Prot
Match: P25776 (Oryzain alpha chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0650000 PE=1 SV=2)

HSP 1 Score: 603.2 bits (1554), Expect = 2.5e-171
Identity = 278/411 (67.64%), Postives = 330/411 (80.29%), Query Frame = 0


Query: 53  QRTDEEVAALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRES----RTYKVGL 112
           +R++EE   LY  W   HGK+YNA+GE+ERR+  F+DNL +IDEHN  +     ++++GL
Sbjct: 30  ERSEEEARRLYAEWKAEHGKSYNAVGEEERRYAAFRDNLRYIDEHNAAADAGVHSFRLGL 89

Query: 113 TRFADLTNEEYRASLSAAK---------SGRYAAALGDDLPDDVDWRKKGAVANVKDQGQ 172
            RFADLTNEEYR +    +         S RY AA  + LP+ VDWR KGAVA +KDQG 
Sbjct: 90  NRFADLTNEEYRDTYLGLRNKPRRERKVSDRYLAADNEALPESVDWRTKGAVAEIKDQGG 149

Query: 173 CGSCWAFSTVAAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGG 232
           CGSCWAFS +AAVEGINQIVTG+LISLSEQELVDCD S+N GCNGGLMDYAF FII+NGG
Sbjct: 150 CGSCWAFSAIAAVEGINQIVTGDLISLSEQELVDCDTSYNEGCNGGLMDYAFDFIINNGG 209

Query: 233 IDTDEDYPYKGRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGR 292
           IDT++DYPYKG+D  CD NRKNAKVVTID YEDV  N E+SL+KAVANQPVSVAIEAGGR
Sbjct: 210 IDTEDDYPYKGKDERCDVNRKNAKVVTIDSYEDVTPNSETSLQKAVANQPVSVAIEAGGR 269

Query: 293 AFQLYQSGVFTGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVAN 352
           AFQLY SG+FTG+CGT LDHGV AVGYGT+NG DYWIVRNSWGK WGE+GY+R+ERN+  
Sbjct: 270 AFQLYSSGIFTGKCGTALDHGVAAVGYGTENGKDYWIVRNSWGKSWGESGYVRMERNI-K 329

Query: 353 STTGKCGIAVEPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGST 412
           +++GKCGIAVEPSYP+K G NPP P  +PPSP  PPT CD Y++C + +TCCCIY+YG  
Sbjct: 330 ASSGKCGIAVEPSYPLKKGENPPNPGPTPPSPTPPPTVCDNYYTCPDSTTCCCIYEYGKY 389

Query: 413 CFAWGCCPLESATCCDDHYSCCPHEYPVCDLEAGTCRASKDSLMGVNLLKR 451
           C+AWGCCPLE ATCCDDHYSCCPHEYP+C+++ GTC  +KDS + V  LKR
Sbjct: 390 CYAWGCCPLEGATCCDDHYSCCPHEYPICNVQQGTCLMAKDSPLAVKALKR 439

[Close]

BLAST of IVF0023421 vs. ExPASy Swiss-Prot
Match: Q9LT78 (Probable cysteine protease RD21C OS=Arabidopsis thaliana OX=3702 GN=RD21C PE=1 SV=1)

HSP 1 Score: 547.7 bits (1410), Expect = 1.2e-154
Identity = 271/417 (64.99%), Postives = 317/417 (76.02%), Query Frame = 0


Query: 54  RTDEEVAALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNR-ESRTYKVGLTRFA 113
           R + E   +YE WLV + K YN LGEKERRFEIFKDNL F++EH+   +RTY+VGLTRFA
Sbjct: 34  RNEAEARRMYERWLVENRKNYNGLGEKERRFEIFKDNLKFVEEHSSIPNRTYEVGLTRFA 93

Query: 114 DLTNEEYRASLSAAKSGR---------YAAALGDDLPDDVDWRKKGAVANVKDQGQCGSC 173
           DLTN+E+RA    +K  R         Y   +GD LPD +DWR KGAV  VKDQG CGSC
Sbjct: 94  DLTNDEFRAIYLRSKMERTRVPVKGEKYLYKVGDSLPDAIDWRAKGAVNPVKDQGSCGSC 153

Query: 174 WAFSTVAAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGIDTD 233
           WAFS + AVEGINQI TGELISLSEQELVDCD S+N GC GGLMDYAF+FII+NGGIDT+
Sbjct: 154 WAFSAIGAVEGINQIKTGELISLSEQELVDCDTSYNDGCGGGLMDYAFKFIIENGGIDTE 213

Query: 234 EDYPYKGRD-GACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQ 293
           EDYPY   D   C+ ++KN +VVTIDGYEDVP+NDE SLKKA+ANQP+SVAIEAGGRAFQ
Sbjct: 214 EDYPYIATDVNVCNSDKKNTRVVTIDGYEDVPQNDEKSLKKALANQPISVAIEAGGRAFQ 273

Query: 294 LYQSGVFTGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTT 353
           LY SGVFTG CGT+LDHGVVAVGYG++ G DYWIVRNSWG +WGE+GY +LERN+  S +
Sbjct: 274 LYTSGVFTGTCGTSLDHGVVAVGYGSEGGQDYWIVRNSWGSNWGESGYFKLERNIKES-S 333

Query: 354 GKCGIAVEPSYPIK-SGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCF 413
           GKCG+A+  SYP K SGSNPPKP A  PSPV     CD+  +C   STCCC+Y+Y   C+
Sbjct: 334 GKCGVAMMASYPTKSSGSNPPKPPA--PSPV----VCDKSNTCPAKSTCCCLYEYNGKCY 393

Query: 414 AWGCCPLESATCCDDHYSCCPHEYPVCDLEAGTCRASKDSLMGVNLLKRLPANQTKR 459
           +WGCCP ESATCCDD  SCCP  YPVCDL+A TCR   +S + +  L R PA  T +
Sbjct: 394 SWGCCPYESATCCDDGSSCCPQSYPVCDLKANTCRMKGNSPLSIKALTRGPAIATTK 443

[Close]

BLAST of IVF0023421 vs. ExPASy Swiss-Prot
Match: P25777 (Oryzain beta chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0670200 PE=1 SV=2)

HSP 1 Score: 540.8 bits (1392), Expect = 1.5e-152
Identity = 280/462 (60.61%), Postives = 335/462 (72.51%), Query Frame = 0


Query: 10  AFLALFLCLSPFSSASHSSTFSIIDENAKHHL-GIPEIPHSDAHQRTDEEVAALYESWLV 69
           A  A FL L    +A+ +   SII  NA+H   G+ E P       T+ E  A Y+ WL 
Sbjct: 5   AAAAAFLLLLIVGAATAAPDMSIISYNAEHGARGLEEGP-------TEAEARAAYDLWLA 64

Query: 70  HH-GKAYNAL-GEKERRFEIFKDNLMFIDEHNR---ESRTYKVGLTRFADLTNEEYRASL 129
            + G + NAL GE ERRF +F DNL F+D HN    E   +++G+ RFADLTNEE+RA+ 
Sbjct: 65  ENGGGSPNALGGEHERRFLVFWDNLKFVDAHNARADERGGFRLGMNRFADLTNEEFRATF 124

Query: 130 SAAKSGRYAAALG--------DDLPDDVDWRKKGAVANVKDQGQCGSCWAFSTVAAVEGI 189
             AK    + A G        ++LP+ VDWR+KGAVA VK+QGQCGSCWAFS V+ VE I
Sbjct: 125 LGAKVAERSRAAGERYRHDGVEELPESVDWREKGAVAPVKNQGQCGSCWAFSAVSTVESI 184

Query: 190 NQIVTGELISLSEQELVDCD-KSFNMGCNGGLMDYAFQFIIDNGGIDTDEDYPYKGRDGA 249
           NQ+VTGE+I+LSEQELV+C     N GCNGGLMD AF FII NGGIDT++DYPYK  DG 
Sbjct: 185 NQLVTGEMITLSEQELVECSTNGQNSGCNGGLMDDAFDFIIKNGGIDTEDDYPYKAVDGK 244

Query: 250 CDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVFTGRCG 309
           CD NR+NAKVV+IDG+EDVP+NDE SL+KAVA+QPVSVAIEAGGR FQLY SGVF+GRCG
Sbjct: 245 CDINRENAKVVSIDGFEDVPQNDEKSLQKAVAHQPVSVAIEAGGREFQLYHSGVFSGRCG 304

Query: 310 TNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTTGKCGIAVEPSYP 369
           T+LDHGVVAVGYGTDNG DYWIVRNSWG  WGE+GY+R+ERN+ N TTGKCGIA+  SYP
Sbjct: 305 TSLDHGVVAVGYGTDNGKDYWIVRNSWGPKWGESGYVRMERNI-NVTTGKCGIAMMASYP 364

Query: 370 IKSGSNPPKPSASPPSPVNPPTE------CDEYFSCDEGSTCCCIYQYGSTCFAWGCCPL 429
            KSG+NPPKPS +PP+P  PP        CD+ FSC  GSTCCC + + + C  WGCCP+
Sbjct: 365 TKSGANPPKPSPTPPTPPTPPPPSAPDHVCDDNFSCPAGSTCCCAFGFRNLCLVWGCCPV 424

Query: 430 ESATCCDDHYSCCPHEYPVCDLEAGTCRASKDSLMGVNLLKR 451
           E ATCC DH SCCP +YPVC+  AGTC ASK+S + V  LKR
Sbjct: 425 EGATCCKDHASCCPPDYPVCNTRAGTCSASKNSPLSVKALKR 458

[Close]

BLAST of IVF0023421 vs. ExPASy TrEMBL
Match: A0A1S3BZW8 (low-temperature-induced cysteine proteinase-like OS=Cucumis melo OX=3656 GN=LOC103495259 PE=3 SV=1)

HSP 1 Score: 960.3 bits (2481), Expect = 3.0e-276
Identity = 469/480 (97.71%), Postives = 469/480 (97.71%), Query Frame = 0


Query: 1   MAISSPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVA 60
           MAISSPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVA
Sbjct: 1   MAISSPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVA 60

Query: 61  ALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYR 120
           ALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYR
Sbjct: 61  ALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYR 120

Query: 121 A-----------SLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQCGSCWAFSTV 180
           A           SLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQCGSCWAFSTV
Sbjct: 121 ARFLGGRFSRKPSLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQCGSCWAFSTV 180

Query: 181 AAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGIDTDEDYPYK 240
           AAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGIDTDEDYPYK
Sbjct: 181 AAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGIDTDEDYPYK 240

Query: 241 GRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVF 300
           GRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVF
Sbjct: 241 GRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVF 300

Query: 301 TGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTTGKCGIAV 360
           TGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTTGKCGIAV
Sbjct: 301 TGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTTGKCGIAV 360

Query: 361 EPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCFAWGCCPLE 420
           EPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCFAWGCCPLE
Sbjct: 361 EPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCFAWGCCPLE 420

Query: 421 SATCCDDHYSCCPHEYPVCDLEAGTCRASKDSLMGVNLLKRLPANQTKRIQKLGKLFVGA 470
           SATCCDDHYSCCPHEYPVCDLEAGTCRASKDSLMGVNLLKRLPANQTKRIQKLGKLFVGA
Sbjct: 421 SATCCDDHYSCCPHEYPVCDLEAGTCRASKDSLMGVNLLKRLPANQTKRIQKLGKLFVGA 480

[Close]

BLAST of IVF0023421 vs. ExPASy TrEMBL
Match: A0A5A7SJU5 (Low-temperature-induced cysteine proteinase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00750 PE=3 SV=1)

HSP 1 Score: 949.1 bits (2452), Expect = 6.8e-273
Identity = 468/488 (95.90%), Postives = 468/488 (95.90%), Query Frame = 0


Query: 1   MAISSPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVA 60
           MAISSPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVA
Sbjct: 1   MAISSPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVA 60

Query: 61  ALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYR 120
           ALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYR
Sbjct: 61  ALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYR 120

Query: 121 A-----------SLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQ--------CG 180
           A           SLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQ         G
Sbjct: 121 ARFLGGRFSRKPSLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQKFVMRSIFIG 180

Query: 181 SCWAFSTVAAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGID 240
           SCWAFSTVAAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGID
Sbjct: 181 SCWAFSTVAAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGID 240

Query: 241 TDEDYPYKGRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAF 300
           TDEDYPYKGRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAF
Sbjct: 241 TDEDYPYKGRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAF 300

Query: 301 QLYQSGVFTGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANST 360
           QLYQSGVFTGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANST
Sbjct: 301 QLYQSGVFTGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANST 360

Query: 361 TGKCGIAVEPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCF 420
           TGKCGIAVEPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCF
Sbjct: 361 TGKCGIAVEPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCF 420

Query: 421 AWGCCPLESATCCDDHYSCCPHEYPVCDLEAGTCRASKDSLMGVNLLKRLPANQTKRIQK 470
           AWGCCPLESATCCDDHYSCCPHEYPVCDLEAGTCRASKDSLMGVNLLKRLPANQTKRIQK
Sbjct: 421 AWGCCPLESATCCDDHYSCCPHEYPVCDLEAGTCRASKDSLMGVNLLKRLPANQTKRIQK 480

[Close]

BLAST of IVF0023421 vs. ExPASy TrEMBL
Match: A0A0A0K4N3 (Cysteine protease OS=Cucumis sativus OX=3659 GN=Csa_7G073400 PE=3 SV=1)

HSP 1 Score: 908.3 bits (2346), Expect = 1.3e-260
Identity = 443/480 (92.29%), Postives = 454/480 (94.58%), Query Frame = 0


Query: 1   MAISSPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVA 60
           MAI SPIFFAFLALF CLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQR DEEVA
Sbjct: 1   MAI-SPIFFAFLALFFCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRPDEEVA 60

Query: 61  ALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYR 120
           ALYESWLVHHGKAYNA+GEKERRFEIFKDNL FIDEHNRESRTYKVGLTRFADLTNEEYR
Sbjct: 61  ALYESWLVHHGKAYNAIGEKERRFEIFKDNLRFIDEHNRESRTYKVGLTRFADLTNEEYR 120

Query: 121 A-----------SLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQCGSCWAFSTV 180
           A            LSAAKSGRYAAALGDDLPDDVDWRKKGAVA VKDQGQCGSCWAFS+V
Sbjct: 121 ARFLGGRFSRKPRLSAAKSGRYAAALGDDLPDDVDWRKKGAVATVKDQGQCGSCWAFSSV 180

Query: 181 AAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGIDTDEDYPYK 240
           AAVEGINQIVTGELI LSEQELVDCDKSFNMGCNGGLMDYAFQFII NGGIDT+EDYPYK
Sbjct: 181 AAVEGINQIVTGELIPLSEQELVDCDKSFNMGCNGGLMDYAFQFIIGNGGIDTEEDYPYK 240

Query: 241 GRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVF 300
           GRD ACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVF
Sbjct: 241 GRDAACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVF 300

Query: 301 TGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTTGKCGIAV 360
           TGRCGT+LDHGVVAVGYGTDNGTDYWIVRNSWGKDWGE+GYIRLERNVAN TTGKCGIAV
Sbjct: 301 TGRCGTDLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGESGYIRLERNVANITTGKCGIAV 360

Query: 361 EPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCFAWGCCPLE 420
           +PSYP KSG+NPPKPSASPPSPV PPTECDEYFSC+EGSTCCCIYQ+GSTCFAWGCCPLE
Sbjct: 361 QPSYPTKSGANPPKPSASPPSPVKPPTECDEYFSCEEGSTCCCIYQFGSTCFAWGCCPLE 420

Query: 421 SATCCDDHYSCCPHEYPVCDLEAGTCRASKDSLMGVNLLKRLPANQTKRIQKLGKLFVGA 470
           SATCCDDHYSCCPHEYPVCDLEAGTCR SKDS MGVNLLKRLPA QTK++QKLGKLFVGA
Sbjct: 421 SATCCDDHYSCCPHEYPVCDLEAGTCRVSKDSSMGVNLLKRLPAIQTKKVQKLGKLFVGA 479

[Close]

BLAST of IVF0023421 vs. ExPASy TrEMBL
Match: A0A6J1D149 (low-temperature-induced cysteine proteinase-like OS=Momordica charantia OX=3673 GN=LOC111016644 PE=3 SV=1)

HSP 1 Score: 794.7 bits (2051), Expect = 2.2e-226
Identity = 387/486 (79.63%), Postives = 422/486 (86.83%), Query Frame = 0


Query: 1   MAISSPIFF---AFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQ---R 60
           MAI S   F   A LALFLC   FSSAS SS+FSIIDENAKHHLG P+I  SDA     R
Sbjct: 1   MAIFSSFSFPILASLALFLCFLSFSSASDSSSFSIIDENAKHHLGFPDIAGSDAGTPPLR 60

Query: 61  TDEEVAALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADL 120
           T E+VAALY+SWLV HGKAYNALGE+ERRFEIFKDNL FIDEHNRE R+Y +GLTRFADL
Sbjct: 61  TQEQVAALYKSWLVKHGKAYNALGERERRFEIFKDNLRFIDEHNREPRSYTLGLTRFADL 120

Query: 121 TNEEYRASL-----------SAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQCGSC 180
           TNEEYRA             SAAK+GRYA++LG DLP+ VDWR+KGAV  VKDQGQCGSC
Sbjct: 121 TNEEYRARFLGGRFSPKPRPSAAKNGRYASSLGGDLPEHVDWREKGAVTAVKDQGQCGSC 180

Query: 181 WAFSTVAAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGIDTD 240
           WAFST+AAVEGINQIVTGELISLSEQELVDCDKS+NMGCNGGLMDYAFQFI+DNGGIDT+
Sbjct: 181 WAFSTIAAVEGINQIVTGELISLSEQELVDCDKSYNMGCNGGLMDYAFQFIVDNGGIDTE 240

Query: 241 EDYPYKGRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQL 300
           EDYPYKGRDG CDPNR+NA VVTIDGYEDVPENDE +LKKAVA+QPVSVAIEAGGRAFQL
Sbjct: 241 EDYPYKGRDGVCDPNRRNANVVTIDGYEDVPENDEGALKKAVASQPVSVAIEAGGRAFQL 300

Query: 301 YQSGVFTGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTTG 360
           YQSGVFTGRCGT+LDHGVVAVGYGT+NG DYWIVRNSWGK+WGENGYIRLERNVAN TTG
Sbjct: 301 YQSGVFTGRCGTDLDHGVVAVGYGTENGLDYWIVRNSWGKNWGENGYIRLERNVANITTG 360

Query: 361 KCGIAVEPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCFAW 420
           KCGIA+EPSYP+K+G NPPKP+ SPPSPV PPTECD+Y+SC EG+TCCCIYQYGSTCF W
Sbjct: 361 KCGIAIEPSYPVKTGKNPPKPAPSPPSPVKPPTECDDYYSCPEGTTCCCIYQYGSTCFGW 420

Query: 421 GCCPLESATCCDDHYSCCPHEYPVCDLEAGTCRASKDSLMGVNLLKRLPANQTKRIQKLG 470
           GCCPLESATCCDD YSCCP EYPVCDL  GTCR SK SL+GV+LLKRLPA     +QKLG
Sbjct: 421 GCCPLESATCCDDQYSCCPREYPVCDLAEGTCRMSKGSLIGVSLLKRLPAKHKGVVQKLG 480

[Close]

BLAST of IVF0023421 vs. ExPASy TrEMBL
Match: A0A6J1JKG6 (low-temperature-induced cysteine proteinase-like OS=Cucurbita maxima OX=3661 GN=LOC111486001 PE=3 SV=1)

HSP 1 Score: 781.9 bits (2018), Expect = 1.4e-222
Identity = 385/485 (79.38%), Postives = 416/485 (85.77%), Query Frame = 0


Query: 1   MAISSPIFF---AFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIP-EIPHSDAHQRTD 60
           MAIS+   F   AFLALFLC S  S AS SS+FSIIDENAKHHL +    PH     R+D
Sbjct: 1   MAISTTFSFSIVAFLALFLCFSSSSLASDSSSFSIIDENAKHHLDVQIGSPH-----RSD 60

Query: 61  EEVAALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTN 120
           EEVAALYESWLVH+GKAYNALGEKERRFEIFKDNL FIDEHNRESR+YKVGL RFADL+N
Sbjct: 61  EEVAALYESWLVHNGKAYNALGEKERRFEIFKDNLRFIDEHNRESRSYKVGLNRFADLSN 120

Query: 121 EEYRA-----------SLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQCGSCWA 180
           EEYRA            LSA KS RYA  LGDDLPD VDWRKKGAV +VKDQGQCGSCWA
Sbjct: 121 EEYRAMFLSGRLSRKNRLSATKSRRYAITLGDDLPDHVDWRKKGAVTDVKDQGQCGSCWA 180

Query: 181 FSTVAAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGIDTDED 240
           FSTVAAVEGINQI TGELISLSEQELVDCD+S+NMGCNGGLMDYAFQFIIDNGGIDT+ED
Sbjct: 181 FSTVAAVEGINQITTGELISLSEQELVDCDRSYNMGCNGGLMDYAFQFIIDNGGIDTEED 240

Query: 241 YPYKGRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQ 300
           YPYKGRDG CDPNR+N+K VTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQ
Sbjct: 241 YPYKGRDGTCDPNRRNSKAVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQ 300

Query: 301 SGVFTGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTTGKC 360
           SGVF G CGT LDHGVVAVGYGT++G DYWIVRNSWGK+WGENGYI+LERNV N TTGKC
Sbjct: 301 SGVFNGLCGTELDHGVVAVGYGTEDGKDYWIVRNSWGKNWGENGYIKLERNVGNITTGKC 360

Query: 361 GIAVEPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCFAWGC 420
           GIA+E SYP K+G NPPKP+ SPPSPV+PP +CDEYFSC EG+TCCCIY+YGSTCF WGC
Sbjct: 361 GIAIEASYPTKTGKNPPKPAPSPPSPVSPPNQCDEYFSCQEGTTCCCIYKYGSTCFGWGC 420

Query: 421 CPLESATCCDDHYSCCPHEYPVCDLEAGTCRASKDSLMGVNLLKRLPANQTKR-IQKLGK 470
           CPLESATCCDDHYSCCPHEYP+CDL+AGTC  SK S  GV LLKR+PA   +  +QKLGK
Sbjct: 421 CPLESATCCDDHYSCCPHEYPICDLDAGTCLKSKGSTTGVKLLKRVPAKYNRGCVQKLGK 480

[Close]

BLAST of IVF0023421 vs. NCBI nr
Match: XP_008454976.1 (PREDICTED: low-temperature-induced cysteine proteinase-like [Cucumis melo])

HSP 1 Score: 957 bits (2475), Expect = 0.0
Identity = 469/480 (97.71%), Postives = 469/480 (97.71%), Query Frame = 0


Query: 1   MAISSPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVA 60
           MAISSPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVA
Sbjct: 1   MAISSPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVA 60

Query: 61  ALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYR 120
           ALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYR
Sbjct: 61  ALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYR 120

Query: 121 A-----------SLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQCGSCWAFSTV 180
           A           SLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQCGSCWAFSTV
Sbjct: 121 ARFLGGRFSRKPSLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQCGSCWAFSTV 180

Query: 181 AAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGIDTDEDYPYK 240
           AAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGIDTDEDYPYK
Sbjct: 181 AAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGIDTDEDYPYK 240

Query: 241 GRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVF 300
           GRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVF
Sbjct: 241 GRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVF 300

Query: 301 TGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTTGKCGIAV 360
           TGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTTGKCGIAV
Sbjct: 301 TGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTTGKCGIAV 360

Query: 361 EPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCFAWGCCPLE 420
           EPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCFAWGCCPLE
Sbjct: 361 EPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCFAWGCCPLE 420

Query: 421 SATCCDDHYSCCPHEYPVCDLEAGTCRASKDSLMGVNLLKRLPANQTKRIQKLGKLFVGA 469
           SATCCDDHYSCCPHEYPVCDLEAGTCRASKDSLMGVNLLKRLPANQTKRIQKLGKLFVGA
Sbjct: 421 SATCCDDHYSCCPHEYPVCDLEAGTCRASKDSLMGVNLLKRLPANQTKRIQKLGKLFVGA 480

[Close]

BLAST of IVF0023421 vs. NCBI nr
Match: KAA0031322.1 (low-temperature-induced cysteine proteinase-like [Cucumis melo var. makuwa] >TYK06773.1 low-temperature-induced cysteine proteinase-like [Cucumis melo var. makuwa])

HSP 1 Score: 946 bits (2446), Expect = 0.0
Identity = 468/488 (95.90%), Postives = 468/488 (95.90%), Query Frame = 0


Query: 1   MAISSPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVA 60
           MAISSPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVA
Sbjct: 1   MAISSPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVA 60

Query: 61  ALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYR 120
           ALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYR
Sbjct: 61  ALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYR 120

Query: 121 A-----------SLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQ--------CG 180
           A           SLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQ         G
Sbjct: 121 ARFLGGRFSRKPSLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQKFVMRSIFIG 180

Query: 181 SCWAFSTVAAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGID 240
           SCWAFSTVAAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGID
Sbjct: 181 SCWAFSTVAAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGID 240

Query: 241 TDEDYPYKGRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAF 300
           TDEDYPYKGRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAF
Sbjct: 241 TDEDYPYKGRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAF 300

Query: 301 QLYQSGVFTGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANST 360
           QLYQSGVFTGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANST
Sbjct: 301 QLYQSGVFTGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANST 360

Query: 361 TGKCGIAVEPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCF 420
           TGKCGIAVEPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCF
Sbjct: 361 TGKCGIAVEPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCF 420

Query: 421 AWGCCPLESATCCDDHYSCCPHEYPVCDLEAGTCRASKDSLMGVNLLKRLPANQTKRIQK 469
           AWGCCPLESATCCDDHYSCCPHEYPVCDLEAGTCRASKDSLMGVNLLKRLPANQTKRIQK
Sbjct: 421 AWGCCPLESATCCDDHYSCCPHEYPVCDLEAGTCRASKDSLMGVNLLKRLPANQTKRIQK 480

[Close]

BLAST of IVF0023421 vs. NCBI nr
Match: XP_004136967.1 (low-temperature-induced cysteine proteinase [Cucumis sativus] >KGN43904.1 hypothetical protein Csa_017340 [Cucumis sativus])

HSP 1 Score: 906 bits (2341), Expect = 0.0
Identity = 440/476 (92.44%), Postives = 451/476 (94.75%), Query Frame = 0


Query: 5   SPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVAALYE 64
           SPIFFAFLALF CLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQR DEEVAALYE
Sbjct: 4   SPIFFAFLALFFCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRPDEEVAALYE 63

Query: 65  SWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYRAS-- 124
           SWLVHHGKAYNA+GEKERRFEIFKDNL FIDEHNRESRTYKVGLTRFADLTNEEYRA   
Sbjct: 64  SWLVHHGKAYNAIGEKERRFEIFKDNLRFIDEHNRESRTYKVGLTRFADLTNEEYRARFL 123

Query: 125 ---------LSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQCGSCWAFSTVAAVE 184
                    LSAAKSGRYAAALGDDLPDDVDWRKKGAVA VKDQGQCGSCWAFS+VAAVE
Sbjct: 124 GGRFSRKPRLSAAKSGRYAAALGDDLPDDVDWRKKGAVATVKDQGQCGSCWAFSSVAAVE 183

Query: 185 GINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGIDTDEDYPYKGRDG 244
           GINQIVTGELI LSEQELVDCDKSFNMGCNGGLMDYAFQFII NGGIDT+EDYPYKGRD 
Sbjct: 184 GINQIVTGELIPLSEQELVDCDKSFNMGCNGGLMDYAFQFIIGNGGIDTEEDYPYKGRDA 243

Query: 245 ACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVFTGRC 304
           ACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVFTGRC
Sbjct: 244 ACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVFTGRC 303

Query: 305 GTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTTGKCGIAVEPSY 364
           GT+LDHGVVAVGYGTDNGTDYWIVRNSWGKDWGE+GYIRLERNVAN TTGKCGIAV+PSY
Sbjct: 304 GTDLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGESGYIRLERNVANITTGKCGIAVQPSY 363

Query: 365 PIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCFAWGCCPLESATC 424
           P KSG+NPPKPSASPPSPV PPTECDEYFSC+EGSTCCCIYQ+GSTCFAWGCCPLESATC
Sbjct: 364 PTKSGANPPKPSASPPSPVKPPTECDEYFSCEEGSTCCCIYQFGSTCFAWGCCPLESATC 423

Query: 425 CDDHYSCCPHEYPVCDLEAGTCRASKDSLMGVNLLKRLPANQTKRIQKLGKLFVGA 469
           CDDHYSCCPHEYPVCDLEAGTCR SKDS MGVNLLKRLPA QTK++QKLGKLFVGA
Sbjct: 424 CDDHYSCCPHEYPVCDLEAGTCRVSKDSSMGVNLLKRLPAIQTKKVQKLGKLFVGA 479

[Close]

BLAST of IVF0023421 vs. NCBI nr
Match: XP_038889032.1 (low-temperature-induced cysteine proteinase-like [Benincasa hispida])

HSP 1 Score: 861 bits (2224), Expect = 1.41e-313
Identity = 424/486 (87.24%), Postives = 444/486 (91.36%), Query Frame = 0


Query: 1   MAISSPIFF---AFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSD---AHQR 60
           MAISS   F   AFLALFLC SPFSSASHSSTFSIIDENAKHH+GIP+I  SD   + QR
Sbjct: 1   MAISSTFSFLTLAFLALFLCFSPFSSASHSSTFSIIDENAKHHMGIPDIADSDHLRSPQR 60

Query: 61  TDEEVAALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADL 120
           TD+EVAALYESWLVHH KAYNALGEKERRFEIFKDNL FIDEHNRESRTYKVGLTRFADL
Sbjct: 61  TDDEVAALYESWLVHHRKAYNALGEKERRFEIFKDNLRFIDEHNRESRTYKVGLTRFADL 120

Query: 121 TNEEYRAS-----------LSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQCGSC 180
           TNEEYRA            LSAAKSGRYAAALGDDLPD VDWRKKGAVA VKDQGQCGSC
Sbjct: 121 TNEEYRARFLGGRFPGKPRLSAAKSGRYAAALGDDLPDHVDWRKKGAVAAVKDQGQCGSC 180

Query: 181 WAFSTVAAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGIDTD 240
           WAFSTVAAVEGINQIVTGELISLSEQELVDCDKS+NMGCNGGLMDYAFQFIIDNGGIDT+
Sbjct: 181 WAFSTVAAVEGINQIVTGELISLSEQELVDCDKSYNMGCNGGLMDYAFQFIIDNGGIDTE 240

Query: 241 EDYPYKGRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQL 300
           EDYPYKG D ACDPNRKNAKVVTIDG+EDVPENDESSLKKAVANQPVSVAIEAGGRAFQL
Sbjct: 241 EDYPYKGHDAACDPNRKNAKVVTIDGFEDVPENDESSLKKAVANQPVSVAIEAGGRAFQL 300

Query: 301 YQSGVFTGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTTG 360
           YQSGVFTGRCGTNLDHGVVAVGYGT+NGTDYWIVRNSWGK+WGE+GYIRLERNVAN TTG
Sbjct: 301 YQSGVFTGRCGTNLDHGVVAVGYGTENGTDYWIVRNSWGKNWGESGYIRLERNVANITTG 360

Query: 361 KCGIAVEPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCFAW 420
           KCGIAV+PSYPIK+GSNPPKP+ASPPSPV PPTECD+Y+SC+EG+TCCCIYQYGSTCF W
Sbjct: 361 KCGIAVQPSYPIKTGSNPPKPAASPPSPVKPPTECDDYYSCEEGTTCCCIYQYGSTCFGW 420

Query: 421 GCCPLESATCCDDHYSCCPHEYPVCDLEAGTCRASKDSLMGVNLLKRLPANQTKRIQKLG 469
           GCCPLESATCCDDHYSCCPHEYP+CDLEAGTC   KDS MGV LLKRLPA +TK IQK G
Sbjct: 421 GCCPLESATCCDDHYSCCPHEYPICDLEAGTCLIDKDSTMGVKLLKRLPAKRTKGIQKFG 480

[Close]

BLAST of IVF0023421 vs. NCBI nr
Match: XP_022147795.1 (low-temperature-induced cysteine proteinase-like [Momordica charantia])

HSP 1 Score: 792 bits (2045), Expect = 2.60e-286
Identity = 382/474 (80.59%), Postives = 417/474 (87.97%), Query Frame = 0


Query: 10  AFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQ---RTDEEVAALYESW 69
           A LALFLC   FSSAS SS+FSIIDENAKHHLG P+I  SDA     RT E+VAALY+SW
Sbjct: 13  ASLALFLCFLSFSSASDSSSFSIIDENAKHHLGFPDIAGSDAGTPPLRTQEQVAALYKSW 72

Query: 70  LVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYRASL--- 129
           LV HGKAYNALGE+ERRFEIFKDNL FIDEHNRE R+Y +GLTRFADLTNEEYRA     
Sbjct: 73  LVKHGKAYNALGERERRFEIFKDNLRFIDEHNREPRSYTLGLTRFADLTNEEYRARFLGG 132

Query: 130 --------SAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQCGSCWAFSTVAAVEGI 189
                   SAAK+GRYA++LG DLP+ VDWR+KGAV  VKDQGQCGSCWAFST+AAVEGI
Sbjct: 133 RFSPKPRPSAAKNGRYASSLGGDLPEHVDWREKGAVTAVKDQGQCGSCWAFSTIAAVEGI 192

Query: 190 NQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGIDTDEDYPYKGRDGAC 249
           NQIVTGELISLSEQELVDCDKS+NMGCNGGLMDYAFQFI+DNGGIDT+EDYPYKGRDG C
Sbjct: 193 NQIVTGELISLSEQELVDCDKSYNMGCNGGLMDYAFQFIVDNGGIDTEEDYPYKGRDGVC 252

Query: 250 DPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVFTGRCGT 309
           DPNR+NA VVTIDGYEDVPENDE +LKKAVA+QPVSVAIEAGGRAFQLYQSGVFTGRCGT
Sbjct: 253 DPNRRNANVVTIDGYEDVPENDEGALKKAVASQPVSVAIEAGGRAFQLYQSGVFTGRCGT 312

Query: 310 NLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTTGKCGIAVEPSYPI 369
           +LDHGVVAVGYGT+NG DYWIVRNSWGK+WGENGYIRLERNVAN TTGKCGIA+EPSYP+
Sbjct: 313 DLDHGVVAVGYGTENGLDYWIVRNSWGKNWGENGYIRLERNVANITTGKCGIAIEPSYPV 372

Query: 370 KSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCFAWGCCPLESATCCD 429
           K+G NPPKP+ SPPSPV PPTECD+Y+SC EG+TCCCIYQYGSTCF WGCCPLESATCCD
Sbjct: 373 KTGKNPPKPAPSPPSPVKPPTECDDYYSCPEGTTCCCIYQYGSTCFGWGCCPLESATCCD 432

Query: 430 DHYSCCPHEYPVCDLEAGTCRASKDSLMGVNLLKRLPANQTKRIQKLGKLFVGA 469
           D YSCCP EYPVCDL  GTCR SK SL+GV+LLKRLPA     +QKLGK+ +G+
Sbjct: 433 DQYSCCPREYPVCDLAEGTCRMSKGSLIGVSLLKRLPAKHKGVVQKLGKMIIGS 486

[Close]

BLAST of IVF0023421 vs. TAIR 10
Match: AT1G47128.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 630.2 bits (1624), Expect = 1.4e-180
Identity = 304/451 (67.41%), Postives = 357/451 (79.16%), Query Frame = 0


Query: 14  LFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVAALYESWLVHHGKA 73
           LFL +   SSA      SII  + KH +       S    R++ EV ++YE+WLV HGKA
Sbjct: 11  LFLAMVAVSSA---VDMSIISYDEKHGV-------STTGGRSEAEVMSIYEAWLVKHGKA 70

Query: 74  --YNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYRASLSAAK---- 133
              N+L EK+RRFEIFKDNL F+DEHN ++ +Y++GLTRFADLTN+EYR+    AK    
Sbjct: 71  QSQNSLVEKDRRFEIFKDNLRFVDEHNEKNLSYRLGLTRFADLTNDEYRSKYLGAKMEKK 130

Query: 134 -----SGRYAAALGDDLPDDVDWRKKGAVANVKDQGQCGSCWAFSTVAAVEGINQIVTGE 193
                S RY A +GD+LP+ +DWRKKGAVA VKDQG CGSCWAFST+ AVEGINQIVTG+
Sbjct: 131 GERRTSLRYEARVGDELPESIDWRKKGAVAEVKDQGGCGSCWAFSTIGAVEGINQIVTGD 190

Query: 194 LISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGIDTDEDYPYKGRDGACDPNRKNA 253
           LI+LSEQELVDCD S+N GCNGGLMDYAF+FII NGGIDTD+DYPYKG DG CD  RKNA
Sbjct: 191 LITLSEQELVDCDTSYNEGCNGGLMDYAFEFIIKNGGIDTDKDYPYKGVDGTCDQIRKNA 250

Query: 254 KVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVFTGRCGTNLDHGVV 313
           KVVTID YEDVP   E SLKKAVA+QP+S+AIEAGGRAFQLY SG+F G CGT LDHGVV
Sbjct: 251 KVVTIDSYEDVPTYSEESLKKAVAHQPISIAIEAGGRAFQLYDSGIFDGSCGTQLDHGVV 310

Query: 314 AVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTTGKCGIAVEPSYPIKSGSNPP 373
           AVGYGT+NG DYWIVRNSWGK WGE+GY+R+ RN+A S++GKCGIA+EPSYPIK+G NPP
Sbjct: 311 AVGYGTENGKDYWIVRNSWGKSWGESGYLRMARNIA-SSSGKCGIAIEPSYPIKNGENPP 370

Query: 374 KPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCFAWGCCPLESATCCDDHYSCCP 433
            P  SPPSP+ PPT+CD Y++C E +TCCC+++YG  CFAWGCCPLE+ATCCDD+YSCCP
Sbjct: 371 NPGPSPPSPIKPPTQCDSYYTCPESNTCCCLFEYGKYCFAWGCCPLEAATCCDDNYSCCP 430

Query: 434 HEYPVCDLEAGTCRASKDSLMGVNLLKRLPA 454
           HEYPVCDL+ GTC  SK+S   V  LKR PA
Sbjct: 431 HEYPVCDLDQGTCLLSKNSPFSVKALKRKPA 450

[Close]

BLAST of IVF0023421 vs. TAIR 10
Match: AT5G43060.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 621.3 bits (1601), Expect = 6.3e-178
Identity = 303/457 (66.30%), Postives = 350/457 (76.59%), Query Frame = 0


Query: 9   FAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVAALYESWLV 68
           F  L+  + L      S++   SII  +  HH+       +    R+D EV  +YE+W+V
Sbjct: 3   FLKLSPMILLLAMIGVSYAMDMSIISYDENHHI-------TTETSRSDSEVERIYEAWMV 62

Query: 69  HHGKA---YNALG-EKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYRASLS 128
            HGK     N LG EK++RFEIFKDNL FIDEHN ++ +YK+GLTRFADLTNEEYR+   
Sbjct: 63  EHGKKKMNQNGLGAEKDQRFEIFKDNLRFIDEHNTKNLSYKLGLTRFADLTNEEYRSMYL 122

Query: 129 AAK--------SGRYAAALGDDLPDDVDWRKKGAVANVKDQGQCGSCWAFSTVAAVEGIN 188
            AK        S RY A +GD LPD VDWRK+GAVA+VKDQG CGSCWAFST+ AVEGIN
Sbjct: 123 GAKPTKRVLKTSDRYQARVGDALPDSVDWRKEGAVADVKDQGSCGSCWAFSTIGAVEGIN 182

Query: 189 QIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGIDTDEDYPYKGRDGACD 248
           +IVTG+LISLSEQELVDCD S+N GCNGGLMDYAF+FII NGGIDT+ DYPYK  DG CD
Sbjct: 183 KIVTGDLISLSEQELVDCDTSYNQGCNGGLMDYAFEFIIKNGGIDTEADYPYKAADGRCD 242

Query: 249 PNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVFTGRCGTN 308
            NRKNAKVVTID YEDVPEN E+SLKKA+A+QP+SVAIEAGGRAFQLY SGVF G CGT 
Sbjct: 243 QNRKNAKVVTIDSYEDVPENSEASLKKALAHQPISVAIEAGGRAFQLYSSGVFDGLCGTE 302

Query: 309 LDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTTGKCGIAVEPSYPIK 368
           LDHGVVAVGYGT+NG DYWIVRNSWG  WGE+GYI++ RN+  + TGKCGIA+E SYPIK
Sbjct: 303 LDHGVVAVGYGTENGKDYWIVRNSWGNRWGESGYIKMARNI-EAPTGKCGIAMEASYPIK 362

Query: 369 SGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCFAWGCCPLESATCCDD 428
            G NPP P  SPPSP+ PPT CD+YFSC E +TCCC+Y+YG  CF WGCCPLE+ATCCDD
Sbjct: 363 KGQNPPNPGPSPPSPIKPPTTCDKYFSCPESNTCCCLYKYGKYCFGWGCCPLEAATCCDD 422

Query: 429 HYSCCPHEYPVCDLEAGTCRASKDSLMGVNLLKRLPA 454
           + SCCPHEYPVCD+  GTC  SK+S   V  LKR PA
Sbjct: 423 NSSCCPHEYPVCDVNRGTCLMSKNSPFSVKALKRTPA 451

[Close]

BLAST of IVF0023421 vs. TAIR 10
Match: AT3G19390.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 547.7 bits (1410), Expect = 8.8e-156
Identity = 271/417 (64.99%), Postives = 317/417 (76.02%), Query Frame = 0


Query: 54  RTDEEVAALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNR-ESRTYKVGLTRFA 113
           R + E   +YE WLV + K YN LGEKERRFEIFKDNL F++EH+   +RTY+VGLTRFA
Sbjct: 34  RNEAEARRMYERWLVENRKNYNGLGEKERRFEIFKDNLKFVEEHSSIPNRTYEVGLTRFA 93

Query: 114 DLTNEEYRASLSAAKSGR---------YAAALGDDLPDDVDWRKKGAVANVKDQGQCGSC 173
           DLTN+E+RA    +K  R         Y   +GD LPD +DWR KGAV  VKDQG CGSC
Sbjct: 94  DLTNDEFRAIYLRSKMERTRVPVKGEKYLYKVGDSLPDAIDWRAKGAVNPVKDQGSCGSC 153

Query: 174 WAFSTVAAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGIDTD 233
           WAFS + AVEGINQI TGELISLSEQELVDCD S+N GC GGLMDYAF+FII+NGGIDT+
Sbjct: 154 WAFSAIGAVEGINQIKTGELISLSEQELVDCDTSYNDGCGGGLMDYAFKFIIENGGIDTE 213

Query: 234 EDYPYKGRD-GACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQ 293
           EDYPY   D   C+ ++KN +VVTIDGYEDVP+NDE SLKKA+ANQP+SVAIEAGGRAFQ
Sbjct: 214 EDYPYIATDVNVCNSDKKNTRVVTIDGYEDVPQNDEKSLKKALANQPISVAIEAGGRAFQ 273

Query: 294 LYQSGVFTGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTT 353
           LY SGVFTG CGT+LDHGVVAVGYG++ G DYWIVRNSWG +WGE+GY +LERN+  S +
Sbjct: 274 LYTSGVFTGTCGTSLDHGVVAVGYGSEGGQDYWIVRNSWGSNWGESGYFKLERNIKES-S 333

Query: 354 GKCGIAVEPSYPIK-SGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCF 413
           GKCG+A+  SYP K SGSNPPKP A  PSPV     CD+  +C   STCCC+Y+Y   C+
Sbjct: 334 GKCGVAMMASYPTKSSGSNPPKPPA--PSPV----VCDKSNTCPAKSTCCCLYEYNGKCY 393

Query: 414 AWGCCPLESATCCDDHYSCCPHEYPVCDLEAGTCRASKDSLMGVNLLKRLPANQTKR 459
           +WGCCP ESATCCDD  SCCP  YPVCDL+A TCR   +S + +  L R PA  T +
Sbjct: 394 SWGCCPYESATCCDDGSSCCPQSYPVCDLKANTCRMKGNSPLSIKALTRGPAIATTK 443

[Close]

BLAST of IVF0023421 vs. TAIR 10
Match: AT1G09850.1 (xylem bark cysteine peptidase 3 )

HSP 1 Score: 436.8 bits (1122), Expect = 2.2e-122
Identity = 212/391 (54.22%), Postives = 268/391 (68.54%), Query Frame = 0


Query: 55  TDEEVAALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNR-ESRTYKVGLTRFAD 114
           + ++++ L++ W   HGK Y +  E+++R +IFKDN  F+ +HN   + TY + L  FAD
Sbjct: 24  SSDDISELFDDWCQKHGKTYGSEEERQQRIQIFKDNHDFVTQHNLITNATYSLSLNAFAD 83

Query: 115 LTNEEYRAS---LSAAKSGRYAAALGDDL------PDDVDWRKKGAVANVKDQGQCGSCW 174
           LT+ E++AS   LS +      A+ G  L      PD VDWRKKGAV NVKDQG CG+CW
Sbjct: 84  LTHHEFKASRLGLSVSAPSVIMASKGQSLGGSVKVPDSVDWRKKGAVTNVKDQGSCGACW 143

Query: 175 AFSTVAAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGIDTDE 234
           +FS   A+EGINQIVTG+LISLSEQEL+DCDKS+N GCNGGLMDYAF+F+I N GIDT++
Sbjct: 144 SFSATGAMEGINQIVTGDLISLSEQELIDCDKSYNAGCNGGLMDYAFEFVIKNHGIDTEK 203

Query: 235 DYPYKGRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLY 294
           DYPY+ RDG C  ++   KVVTID Y  V  NDE +L +AVA QPVSV I    RAFQLY
Sbjct: 204 DYPYQERDGTCKKDKLKQKVVTIDSYAGVKSNDEKALMEAVAAQPVSVGICGSERAFQLY 263

Query: 295 QSGVFTGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTTGK 354
            SG+F+G C T+LDH V+ VGYG+ NG DYWIV+NSWGK WG +G++ ++RN  NS  G 
Sbjct: 264 SSGIFSGPCSTSLDHAVLIVGYGSQNGVDYWIVKNSWGKSWGMDGFMHMQRNTENS-DGV 323

Query: 355 CGIAVEPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCFAWG 414
           CGI +  SYPIK+  NPP P  SPP     PT+C+ +  C  G TCCC  +    CF+W 
Sbjct: 324 CGINMLASYPIKTHPNPPPP--SPPG----PTKCNLFTYCSSGETCCCARELFGLCFSWK 383

Query: 415 CCPLESATCCDDHYSCCPHEYPVCDLEAGTC 436
           CC +ESA CC D   CCPH+YPVCD     C
Sbjct: 384 CCEIESAVCCKDGRHCCPHDYPVCDTTRSLC 407

[Close]

BLAST of IVF0023421 vs. TAIR 10
Match: AT4G36880.1 (cysteine proteinase1 )

HSP 1 Score: 432.6 bits (1111), Expect = 4.1e-121
Identity = 224/370 (60.54%), Postives = 273/370 (73.78%), Query Frame = 0


Query: 12  LALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVAALYESWLVHHG 71
           L+L L     S AS     SII++    HL +P    SD   RTDEEV ++Y  W   HG
Sbjct: 8   LSLLLLYVVVSLASGDE--SIIND----HLQLP----SDGKWRTDEEVRSIYLQWSAEHG 67

Query: 72  KAYN----ALGEKERRFEIFKDNLMFIDEHNRESR--TYKVGLTRFADLTNEEYRASLSA 131
           K  N     + ++++RF IFKDNL FID HN  ++  TYK+GLT+F DLTN+EYR     
Sbjct: 68  KTNNNNNGIINDQDKRFNIFKDNLRFIDLHNENNKNATYKLGLTKFTDLTNDEYRKLYLG 127

Query: 132 AK-------------SGRYAAAL-GDDLPDDVDWRKKGAVANVKDQGQCGSCWAFSTVAA 191
           A+             + +Y+AA+ G ++P+ VDWR+KGAV  +KDQG CGSCWAFST AA
Sbjct: 128 ARTEPARRIAKAKNVNQKYSAAVNGKEVPETVDWRQKGAVNPIKDQGTCGSCWAFSTTAA 187

Query: 192 VEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGIDTDEDYPYKGR 251
           VEGIN+IVTGELISLSEQELVDCDKS+N GCNGGLMDYAFQFI+ NGG++T++DYPY+G 
Sbjct: 188 VEGINKIVTGELISLSEQELVDCDKSYNQGCNGGLMDYAFQFIMKNGGLNTEKDYPYRGF 247

Query: 252 DGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVFTG 311
            G C+   KN++VV+IDGYEDVP  DE++LKKA++ QPVSVAIEAGGR FQ YQSG+FTG
Sbjct: 248 GGKCNSFLKNSRVVSIDGYEDVPTKDETALKKAISYQPVSVAIEAGGRIFQHYQSGIFTG 307

Query: 312 RCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTTGKCGIAVEP 362
            CGTNLDH VVAVGYG++NG DYWIVRNSWG  WGE GYIR+ERN+A S +GKCGIAVE 
Sbjct: 308 SCGTNLDHAVVAVGYGSENGVDYWIVRNSWGPRWGEEGYIRMERNLAASKSGKCGIAVEA 367

The following BLAST results are available for this feature:

BLAST of IVF0023421 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P43297	1.9e-179	67.41	Cysteine proteinase RD21A OS=Arabidopsis thaliana OX=3702 GN=RD21A PE=1 SV=1	[more]
Q9FMH8	8.8e-177	66.30	Probable cysteine protease RD21B OS=Arabidopsis thaliana OX=3702 GN=RD21B PE=1 S...	[more]
P25776	2.5e-171	67.64	Oryzain alpha chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0650000 PE=...	[more]
Q9LT78	1.2e-154	64.99	Probable cysteine protease RD21C OS=Arabidopsis thaliana OX=3702 GN=RD21C PE=1 S...	[more]
P25777	1.5e-152	60.61	Oryzain beta chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0670200 PE=1...	[more]

BLAST of IVF0023421 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BZW8	3.0e-276	97.71	low-temperature-induced cysteine proteinase-like OS=Cucumis melo OX=3656 GN=LOC1...	[more]
A0A5A7SJU5	6.8e-273	95.90	Low-temperature-induced cysteine proteinase-like OS=Cucumis melo var. makuwa OX=...	[more]
A0A0A0K4N3	1.3e-260	92.29	Cysteine protease OS=Cucumis sativus OX=3659 GN=Csa_7G073400 PE=3 SV=1	[more]
A0A6J1D149	2.2e-226	79.63	low-temperature-induced cysteine proteinase-like OS=Momordica charantia OX=3673 ...	[more]
A0A6J1JKG6	1.4e-222	79.38	low-temperature-induced cysteine proteinase-like OS=Cucurbita maxima OX=3661 GN=...	[more]

BLAST of IVF0023421 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008454976.1	0.0	97.71	PREDICTED: low-temperature-induced cysteine proteinase-like [Cucumis melo]	[more]
KAA0031322.1	0.0	95.90	low-temperature-induced cysteine proteinase-like [Cucumis melo var. makuwa] >TYK...	[more]
XP_004136967.1	0.0	92.44	low-temperature-induced cysteine proteinase [Cucumis sativus] >KGN43904.1 hypoth...	[more]
XP_038889032.1	1.41e-313	87.24	low-temperature-induced cysteine proteinase-like [Benincasa hispida]	[more]
XP_022147795.1	2.60e-286	80.59	low-temperature-induced cysteine proteinase-like [Momordica charantia]	[more]

BLAST of IVF0023421 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G47128.1	1.4e-180	67.41	Granulin repeat cysteine protease family protein	[more]
AT5G43060.1	6.3e-178	66.30	Granulin repeat cysteine protease family protein	[more]
AT3G19390.1	8.8e-156	64.99	Granulin repeat cysteine protease family protein	[more]
AT1G09850.1	2.2e-122	54.22	xylem bark cysteine peptidase 3	[more]
AT4G36880.1	4.1e-121	60.54	cysteine proteinase1	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000668	Peptidase C1A, papain C-terminal	PRINTS	PR00705	PAPAIN	coord: 299..309 score: 57.41 coord: 314..320 score: 73.06 coord: 157..172 score: 66.81
IPR000668	Peptidase C1A, papain C-terminal	SMART	SM00645	pept_c1	coord: 139..355 e-value: 3.2E-128 score: 441.9
IPR000668	Peptidase C1A, papain C-terminal	PFAM	PF00112	Peptidase_C1	coord: 139..355 e-value: 8.8E-86 score: 287.5
IPR000118	Granulin	SMART	SM00277	GRAN_2	coord: 378..435 e-value: 4.7E-26 score: 102.6
IPR000118	Granulin	PFAM	PF00396	Granulin	coord: 389..435 e-value: 3.9E-10 score: 39.9
IPR013201	Cathepsin propeptide inhibitor domain (I29)	SMART	SM00848	Inhibitor_I29_2	coord: 63..119 e-value: 3.0E-25 score: 99.9
IPR013201	Cathepsin propeptide inhibitor domain (I29)	PFAM	PF08246	Inhibitor_I29	coord: 63..119 e-value: 1.6E-17 score: 63.7
None	No IPR available	GENE3D	3.90.70.10	Cysteine proteinases	coord: 45..357 e-value: 1.9E-120 score: 404.2
None	No IPR available	PANTHER	PTHR12411	CYSTEINE PROTEASE FAMILY C1-RELATED	coord: 44..385
None	No IPR available	PANTHER	PTHR12411:SF749	CYSTEINE PROTEASE	coord: 44..385
None	No IPR available	SUPERFAMILY	57277	Granulin repeat	coord: 375..409
IPR037277	Granulin superfamily	GENE3D	2.10.25.160	Granulin	coord: 375..438 e-value: 5.1E-13 score: 51.1
IPR025661	Cysteine peptidase, asparagine active site	PROSITE	PS00640	THIOL_PROTEASE_ASN	coord: 314..333
IPR025660	Cysteine peptidase, histidine active site	PROSITE	PS00639	THIOL_PROTEASE_HIS	coord: 297..307
IPR000169	Cysteine peptidase, cysteine active site	PROSITE	PS00139	THIOL_PROTEASE_CYS	coord: 157..168
IPR039417	Papain-like cysteine endopeptidase	CDD	cd02248	Peptidase_C1A	coord: 140..354 e-value: 4.58073E-113 score: 329.97
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	54001	Cysteine proteinases	coord: 55..355

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
IVF0023421.2	IVF0023421.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051603	proteolysis involved in cellular protein catabolic process
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005615	extracellular space
cellular_component	GO:0005764	lysosome
molecular_function	GO:0004197	cysteine-type endopeptidase activity
molecular_function	GO:0008234	cysteine-type peptidase activity