IVF0020638 (gene) Melon (IVF77) v1

Overview
NameIVF0020638
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionReticulocalbin-2
Locationchr06: 2102070 .. 2106209 (-)
RNA-Seq ExpressionIVF0020638
SyntenyIVF0020638
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTATTGCAAAGCTTAGTGAACGGTACTCCGTTCATCTTCTTCCCCTACCCTAACCTCCACCCATTTTCCCCTTCTGTTTTCTCAAATGTGGCCTTCTCTAGGTATCCGTTTTCCCCATCTAACCTTATCTCGATCCCTTCCGCCGCCGAAAAAATGGGGAAGGCGGTGGTATATGTTCTTACAGCCACCGCCTTCGCTGTCTTCTTCCTAATTTCTCCCTCCAATTTCCACAATCGGAACTATCAACAACCAACTCGTCGTCTAGGATTCAAATTCCCAAACCCTACTTTCGATCCTCTTGTTACGGAAATGGAGAGATTAGAAGCAGAGGAACGTGGTGAAAATGCGATTGGAATGGAAAATCAAAATCATAAGATTACTGATGCTTATAGAAATTACTACGATGAAGGGAGATTGAACATTTCGTTGAGGTTACTTGTGTTGTTTCCTTTGCTGGATAATTCGCCTAAAGATGGGGTTATCAGTTACGAGGAGTTGAGTGATTGGATTAATGGACAAGCTATTGAAAGATTGAATTATAGAACGACGAAACAATTGGAGTTCTACGACAAGAATGGAGATAACGCCATTTCATTCCATGAATATCTGCCGCAGTTCACTAAGGAAGATATTGGTACGCGTTTCTTCATTTCTTTGATTATGTCTACTTTCTACGAATGTTCTCAGGAAGTACACGATTTAGATCGAAAGCAAAAATATTTTATTTTGGTGATTTTGGGTTTAATTTCTTTCTAGTGTTGTGTTTCATAATGTTACATTTTGAAGGTTTTTGTTTCAATTTAGTTTGTTTTTGAAGGTTTGAATTTTGTTTCTATTTATACTTAGGTTTATAACCCTTTTTAATCTTCATTTTATGTTAATGTCTTATTTATTATTTTAAATAAATTAATTTCAAAGAATTAAAATTAATTAAACATCACCGTTTTTGTGTCTTTATTAGATTTTAAATTTTCGTTTCAAAATTATTAGCAAAGATGGGAGGGAGTATTTGTGAGTTGGATTGAAGTACTTTTTAGGCCCTACCTTATTATTCAGGTCCTATATATTTCTTCTACCGAAATAATTTGAATTACAAATTTAACTCAATCCAACCATAGAATTTTTTTATTTAATTTTTTCCAGTTTGCTTAGACCATTTATGTAATTTTTTGTTCTAAATGTCAATGCATACATTTGTTTTAATTATTTTTACATAGATTAAAAACTCAATTTTAATTTATATAATAGAAAATAAATTGCAAAAAAAAAAAAACAAATTTTAAGAATCGGAGGAGAATAAATTATCAAAACATTACTGAAATAAAATAAAATAAAATAAAGATTAGGAATAGAATAGTACCATGCAAATGGAAATTTTAAAAAATTGACAAAAATAACAAATTATTTATCTTCACGAAAAAAAACAGTAGGTTAAATCTTTAGTGCATTTTGTTTTTTTTTTTTTTTTTTCTTTCTTCAAGTCAAAAAAGAATATACGTTTGAAAGTTAGTAATAAATTTGGGTTAGTTTGGTATGATTTAGATGAACCGCTGAACAGTTCAACTTAGTCCAAATGATAACCTAATTTAAATAAAGTTAATTTTTTGAATGCTCCTAATAAAATGTATATATTTTGAAATTTTAGGGATGGAATTGAACCAAAAGTACTAACACTTTGAAGCCTAAAAACTAAATTGAAATCAAATTCAAAACTTAAAAAGAATAATATTTTTTAGATTTGAGATGCAAAAACCTAATAACCAAAAAAACTTATTTCACCTAATTAAAATATAGAAGTGTAAGGAATCTGTTGAGCAAAAATGTTTGACTGAAAAATTGATAATAGGAACAATGTGAGTGAGGAAAAGTACAGTACATTAGATTGAGTACAAAGGAACAACATTTAGACATGTAAAACCTTTTATCCAACATTGAGACGAATGGGAACTTAGTAATTTTTCCAAAAAAATTGTATATCACTTTAAGAAAAAGCAGAAGGTAACTTTTGTAAGTCAGGCTATTATTTTGACATTGTGGGAGTGAGTGAAATAAAATACTGAAAATGATATTATTAAATGAAACAGCAAGAAATGACACGGGATATGGAGAAGCTGGATGGTGGATAAAGCAATTCACAAATGCAGATGTTGACCAGAATGGCTTACTACATTTTGATGAACTGAAAGAGTAATGTCCCATCTTCCTCTCCCATAAATAATAAGGCCTACTTCACTATTTTTAACCTTAATTACTACACATTAATATATCTACATATAAAGCGAACTGTCACATGGCAGCGTTTAGTTAGGTGTAGTATGGACGCACAATAGATTTATACTCTTCTTTTCCAAATTTTATTACAAAATTATCATTAACATTTCGTAGTGTGTCAAAATTAGAGGAAAATCGAGACCTTGGACACGTGTGGTGATTATCACCCAGAACGGTGTGGTAGTGTTCAAGTTTCATTCTTCATTCTACGTCACCATCATATTCTTTCAAGTTTCAATTCACACTCAAATGTAGCATTGTAATTTTTTAAATGATTTATAATTTTTCTAAGTTTAAAGTCCTTTAGTTTGTTTAATTTTAGTCTCTAATTTTCAATAAATCTTAATTTTAGTCTCTAATTTTCAATAAATCTTAAATTTAGTCTCCGATATTAATTTGTTTGCTTCTAAAAATTTATTGTGATCTTGCACTATTTTTCTATAAGTTCTTAAAAATACTAATGGTTTAATCAATTCAGGTAATGACTAAAATTAAAATTTAATTAAAAGTACAGACTTAAACTGAATCTAACCGAGACAAAAAGGTTTTCGATTTTTATTTTTAAAGGGTATGAGATACTTGTATCTTGCAGTTTCCTCCACCCAGAAGATAGCAGCAATTACCGAATACAAAACTGGCTGTTAGCACAGAAAATGAAGTAACTTCTTCCCCTATACTTGAATCCATAAACTTCTCTCATCTTCATTGTAATGTAAAAAGAAAACCAAACCATCATCTTCCTGTTTAATTCATTCATATGCAGGCGCATGGACCATGACAGAGATGGAAAGCTCAATTTTGATGAATTCCTTCACCATACATACAATATATACAAGAACTATATCGAATTTGAAACTCAAGGCGACGACATACCGACCGCTGAAGAGAAGTTTGATGAGCTTGATCTTGACGAGGATGAGTATGGCAATGCATTTGTTTGGAGAATTATTTTTTGGGAAATTTTATATATACCAATGTGATAAAAGTTTCTATCAGCTCGTCGAAATGACTTTTTTTTTCTAGTTACTATTTTAGTTTTTATACTTTTGACGTTTAATTTTAGTGATACTTTCAATATGTTCATTTTAGTTTACACACTTTTAACTTTGGTTCTTGTAATTTCAAAATGTTCATTTTGGTTTTTGTACTTTTGAGAGTGACCGTGTTGATCACTTTATTTCATTTTTATATCATTTTCAAGGGACCAAAGTTGTCACATTTTCTAAAGTTCAAGGACCAAAATGAATAAAAAACAAAAAGTATAGGGACCAACGTAATATTTAGACTTGTTTCGGTGTATTTGAAAATATTTATAATGGTTTTGTTGGTTCTCTCTTATTTATGTTGTTAATATATTTCTTATATCAAACATTTTTGTGCAGAGTGCTGTCAGTGGAAGAAATAAGACCGTTGTTCCAGTATCTTCATCCTGGAGAAGTGTCTTATGCTCAACATTACACAAGTCATTTGATTAATGAGGTACTTAATTAGTAGTGGAATTGAAGAGATTAAGTTAAAATAATCAAACTCAAAGATGAGTTAATAATGTGTTAGTTAATGTGTGGTAATTAAAATTGAACAATGCAGGCTGATGACAATAAAGATGGGTACTTAACAATTGATGAAATGCTTAATCATGAGTATGCTTTCTACAGCACAGTATATGAAAATCAGAATGGAGATTATGAAGATGATTATTACCATGATGAACTTTGATTTTTCTTAATTTTTTGCCCCACTTTTTGAAGCTTAGATTTTAGATTTCATTTGCATATAAAGTGTATTGGGTTACAATTTTACTAAACCCTAAACCCACCCCTTTGGATTTTGATTCATTTTCAACCAGTAATTGTTGTAGAGAAAGCTCC

mRNA sequence

ATTATTGCAAAGCTTAGTGAACGGTACTCCGTTCATCTTCTTCCCCTACCCTAACCTCCACCCATTTTCCCCTTCTGTTTTCTCAAATGTGGCCTTCTCTAGGTATCCGTTTTCCCCATCTAACCTTATCTCGATCCCTTCCGCCGCCGAAAAAATGGGGAAGGCGGTGGTATATGTTCTTACAGCCACCGCCTTCGCTGTCTTCTTCCTAATTTCTCCCTCCAATTTCCACAATCGGAACTATCAACAACCAACTCGTCGTCTAGGATTCAAATTCCCAAACCCTACTTTCGATCCTCTTGTTACGGAAATGGAGAGATTAGAAGCAGAGGAACGTGGTGAAAATGCGATTGGAATGGAAAATCAAAATCATAAGATTACTGATGCTTATAGAAATTACTACGATGAAGGGAGATTGAACATTTCGTTGAGGTTACTTGTGTTGTTTCCTTTGCTGGATAATTCGCCTAAAGATGGGGTTATCAGTTACGAGGAGTTGAGTGATTGGATTAATGGACAAGCTATTGAAAGATTGAATTATAGAACGACGAAACAATTGGAGTTCTACGACAAGAATGGAGATAACGCCATTTCATTCCATGAATATCTGCCGCAGTTCACTAAGGAAGATATTGCAAGAAATGACACGGGATATGGAGAAGCTGGATGGTGGATAAAGCAATTCACAAATGCAGATGTTGACCAGAATGGCTTACTACATTTTGATGAACTGAAAGATTTCCTCCACCCAGAAGATAGCAGCAATTACCGAATACAAAACTGGCTGTTAGCACAGAAAATGAAGCGCATGGACCATGACAGAGATGGAAAGCTCAATTTTGATGAATTCCTTCACCATACATACAATATATACAAGAACTATATCGAATTTGAAACTCAAGGCGACGACATACCGACCGCTGAAGAGAAGTTTGATGAGCTTGATCTTGACGAGGATGAAGTGCTGTCAGTGGAAGAAATAAGACCGTTGTTCCAGTATCTTCATCCTGGAGAAGTGTCTTATGCTCAACATTACACAAGTCATTTGATTAATGAGGCTGATGACAATAAAGATGGGTACTTAACAATTGATGAAATGCTTAATCATGAGTATGCTTTCTACAGCACAGTATATGAAAATCAGAATGGAGATTATGAAGATGATTATTACCATGATGAACTTTGATTTTTCTTAATTTTTTGCCCCACTTTTTGAAGCTTAGATTTTAGATTTCATTTGCATATAAAGTGTATTGGGTTACAATTTTACTAAACCCTAAACCCACCCCTTTGGATTTTGATTCATTTTCAACCAGTAATTGTTGTAGAGAAAGCTCC

Coding sequence (CDS)

ATGGGGAAGGCGGTGGTATATGTTCTTACAGCCACCGCCTTCGCTGTCTTCTTCCTAATTTCTCCCTCCAATTTCCACAATCGGAACTATCAACAACCAACTCGTCGTCTAGGATTCAAATTCCCAAACCCTACTTTCGATCCTCTTGTTACGGAAATGGAGAGATTAGAAGCAGAGGAACGTGGTGAAAATGCGATTGGAATGGAAAATCAAAATCATAAGATTACTGATGCTTATAGAAATTACTACGATGAAGGGAGATTGAACATTTCGTTGAGGTTACTTGTGTTGTTTCCTTTGCTGGATAATTCGCCTAAAGATGGGGTTATCAGTTACGAGGAGTTGAGTGATTGGATTAATGGACAAGCTATTGAAAGATTGAATTATAGAACGACGAAACAATTGGAGTTCTACGACAAGAATGGAGATAACGCCATTTCATTCCATGAATATCTGCCGCAGTTCACTAAGGAAGATATTGCAAGAAATGACACGGGATATGGAGAAGCTGGATGGTGGATAAAGCAATTCACAAATGCAGATGTTGACCAGAATGGCTTACTACATTTTGATGAACTGAAAGATTTCCTCCACCCAGAAGATAGCAGCAATTACCGAATACAAAACTGGCTGTTAGCACAGAAAATGAAGCGCATGGACCATGACAGAGATGGAAAGCTCAATTTTGATGAATTCCTTCACCATACATACAATATATACAAGAACTATATCGAATTTGAAACTCAAGGCGACGACATACCGACCGCTGAAGAGAAGTTTGATGAGCTTGATCTTGACGAGGATGAAGTGCTGTCAGTGGAAGAAATAAGACCGTTGTTCCAGTATCTTCATCCTGGAGAAGTGTCTTATGCTCAACATTACACAAGTCATTTGATTAATGAGGCTGATGACAATAAAGATGGGTACTTAACAATTGATGAAATGCTTAATCATGAGTATGCTTTCTACAGCACAGTATATGAAAATCAGAATGGAGATTATGAAGATGATTATTACCATGATGAACTTTGA

Protein sequence

MGKAVVYVLTATAFAVFFLISPSNFHNRNYQQPTRRLGFKFPNPTFDPLVTEMERLEAEERGENAIGMENQNHKITDAYRNYYDEGRLNISLRLLVLFPLLDNSPKDGVISYEELSDWINGQAIERLNYRTTKQLEFYDKNGDNAISFHEYLPQFTKEDIARNDTGYGEAGWWIKQFTNADVDQNGLLHFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDRDGKLNFDEFLHHTYNIYKNYIEFETQGDDIPTAEEKFDELDLDEDEVLSVEEIRPLFQYLHPGEVSYAQHYTSHLINEADDNKDGYLTIDEMLNHEYAFYSTVYENQNGDYEDDYYHDEL
Homology
BLAST of IVF0020638 vs. ExPASy Swiss-Prot
Match: J3S9D9 (Reticulocalbin-2 OS=Crotalus adamanteus OX=8729 PE=1 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 3.8e-12
Identity = 69/263 (26.24%), Postives = 123/263 (46.77%), Query Frame = 0

Query: 93  RLLVLFPLLDNSPKDGVISYEELSDWINGQAIERLNYRTTKQLEFYDKNGDNAISFHEY- 152
           RL V+   +D    DG ++  ELS WI       +     +Q + YDK+GD  +S+ EY 
Sbjct: 54  RLKVIISRID-VDLDGFLTEAELSSWIQHSFKSYIIEDAKQQFQHYDKDGDGRVSWEEYN 113

Query: 153 LPQFTKEDIARNDTGYGEAGWWI---------KQFTNADVDQNGLLHFDELKDFLHPEDS 212
           +  + +      DT   +A             K+F  A+ D +  L F+E   F HPE++
Sbjct: 114 IQMYDRVIDFEEDTTLDDAEEESFRQLHLKDKKRFQKANKDGDSHLDFEEFAAFEHPEEA 173

Query: 213 SNYRIQNWLLAQKMKRMDHDRDGKLNFDEFLHHTYNIYKNYIEFETQGDDIPTAEEKF-D 272
               ++ +++ + ++  D D DG ++  EFL      Y+     +   + I   E++F +
Sbjct: 174 D--YMKEFVIQESLEEHDKDGDGFVSLQEFL----GDYRRDPAAKEDPEWIVVEEDRFKN 233

Query: 273 ELDLDEDEVLSVEEIRPLFQYLHPGEVSYAQHYTSHLINEADDNKDGYLTIDEMLNHEYA 332
           + D D+D  LS +E   L  ++ P     AQ    HL++E D + D  L+ +E+L ++  
Sbjct: 234 DYDKDKDGKLSPKE---LLTWVMPNNEGLAQEEAVHLLDEMDLDGDRRLSANEILENQDL 293

Query: 333 FYSTVYENQNGDYED-DYYHDEL 344
           F ++   +      D  +YH+EL
Sbjct: 294 FLNSEATDYGRQLHDKSFYHEEL 306

BLAST of IVF0020638 vs. ExPASy Swiss-Prot
Match: Q8BP92 (Reticulocalbin-2 OS=Mus musculus OX=10090 GN=Rcn2 PE=1 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 3.8e-12
Identity = 72/256 (28.12%), Postives = 115/256 (44.92%), Query Frame = 0

Query: 103 NSPKDGVISYEELSDWINGQAIERLNYRTTKQLEFYDKNGDNAISFHEYLPQF------T 162
           +S  DG ++  ELS WI             +Q   YDKN D A+++ EY  Q        
Sbjct: 77  DSDSDGFLTENELSQWIQMSFKHYAMQEAKQQFVEYDKNSDGAVTWDEYNIQMYDRVIDF 136

Query: 163 KEDIARNDTGYGEAGWW----IKQFTNADVDQNGLLHFDELKDFLHPEDSSNYRIQNWLL 222
            E+ A +DT  G          K+F  A+ D    L  +E   F HPE+     +  +++
Sbjct: 137 DENTALDDTEEGSFRQLHLKDKKRFEKANQDSGPGLSLEEFIAFEHPEEVD--YMTEFVI 196

Query: 223 AQKMKRMDHDRDGKLNFDEFLHHTYNIYKNYIEFETQGDD---IPTAEEKF-DELDLDED 282
            + ++  D + DG ++ +EFL        +Y    T  +D   I   +++F ++ D D D
Sbjct: 197 QEALEEHDKNGDGFVSLEEFL-------GDYRRDPTANEDPEWILVEKDRFVNDYDKDND 256

Query: 283 EVLSVEEIRPLFQYLHPGEVSYAQHYTSHLINEADDNKDGYLTIDEMLNHEYAFYSTVYE 342
             L  +E   L  ++ P     AQ    HLI+E D N D  L+ +E+L ++  F ++   
Sbjct: 257 GRLDPQE---LLSWVVPNNQGIAQEEALHLIDEMDLNSDKKLSEEEILENQDLFLTSEAT 316

Query: 343 NQNGDYEDDY-YHDEL 344
           +      DDY YHDEL
Sbjct: 317 DYGRQLHDDYFYHDEL 320

BLAST of IVF0020638 vs. ExPASy Swiss-Prot
Match: B5X4E0 (Calumenin-B OS=Salmo salar OX=8030 GN=calub PE=2 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 1.5e-11
Identity = 63/240 (26.25%), Postives = 117/240 (48.75%), Query Frame = 0

Query: 93  RLLVLFPLLDNSPKDGVISYEELSDWINGQAIERLNYRTTKQLEFYDKNGDNAISFHEY- 152
           RL +L   +D   KDG +S EE+  WI       +     +Q + +D NGD  +S+ EY 
Sbjct: 73  RLGMLVERIDED-KDGYVSVEEMKKWIKHSQKRWIYDDVDRQWKGHDHNGDGLVSWEEYK 132

Query: 153 -------LPQFTKED-------IARNDTGYGEAGWWIKQFTNADVDQNGLLHFDELKDFL 212
                  L     ED       I+R++          ++F  +D+D +   + +E   FL
Sbjct: 133 NATYGYILDDPDPEDGFSYRQMISRDE----------RRFKMSDLDADLKANKEEFTAFL 192

Query: 213 HPEDSSNYRIQNWLLAQKMKRMDHDRDGKLNFDEFLHHTYNIYKNYIEFETQGDDIPTAE 272
           HPE+  +  +++ ++ + M+ +D + DG ++ +E++   YN   +  E E     + T  
Sbjct: 193 HPEEYDH--MKDIVVLETMEDIDKNGDGFIDLEEYIGDMYNQEGDPSEPEW----VRTER 252

Query: 273 EKFDEL-DLDEDEVLSVEEIRPLFQYLHPGEVSYAQHYTSHLINEADDNKDGYLTIDEML 317
           E+F E  D ++D  +  EE +    ++ P +  +A+    HL+ E+D++KDG LT  E++
Sbjct: 253 EQFTEFRDTNKDGRMDKEETK---DWILPSDYDHAEAEAKHLVYESDNDKDGKLTKAEIV 292

BLAST of IVF0020638 vs. ExPASy Swiss-Prot
Match: O93434 (Reticulocalbin-1 OS=Takifugu rubripes OX=31033 GN=rcn1 PE=3 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 2.5e-11
Identity = 69/263 (26.24%), Postives = 122/263 (46.39%), Query Frame = 0

Query: 97  LFPLLDNSPKDG--VISYEELSDWINGQAIERLNYRTTKQLEFYDKNGDNAISFHEYLPQ 156
           L  ++D    DG   I+ +EL  WI       +     K    YD N DN IS+ EY  Q
Sbjct: 75  LSKIVDRIDGDGNSYITTDELKAWIKRVQKRYVYENVVKVWADYDLNKDNKISWEEY-KQ 134

Query: 157 FTKEDIARNDTGYGEAGWWI----------KQFTNADVDQNGLLHFDELKDFLHPEDSSN 216
            T      N   + E               ++F  AD+D +   + +E   FLHPE+  +
Sbjct: 135 ATYGYYLSNPEEFDETTDQFSFKKMLPRDERRFKRADLDGDSAANREEFTSFLHPEEFEH 194

Query: 217 YRIQNWLLAQKMKRMDHDRDGKLNFDEFLHHTYNIYKNYIEFETQGDD---IPTAEEKFD 276
             +++ ++ + ++ +D + DG ++ DE++        +    E +G +   + T  E+F 
Sbjct: 195 --MKDIVVLETLEDIDKNSDGHVDEDEYI-------ADMFAHEDRGPEPEWVKTEREQFS 254

Query: 277 EL-DLDEDEVLSVEEIRPLFQYLHPGEVSYAQHYTSHLINEADDNKDGYLTIDEMLNHEY 336
           +  DL++D  + ++EIR    ++ P +  +AQ    HL+ E+D +KD  LT +E+L++  
Sbjct: 255 DFRDLNKDGKMDLDEIR---HWIMPQDYDHAQAEARHLVYESDKDKDQMLTKEEILDNWN 314

Query: 337 AFYSTVYENQNGDYEDDYYHDEL 344
            F  +   N   D   +  HDEL
Sbjct: 315 MFVGSQATNYGEDLTRN--HDEL 322

BLAST of IVF0020638 vs. ExPASy Swiss-Prot
Match: Q62703 (Reticulocalbin-2 OS=Rattus norvegicus OX=10116 GN=Rcn2 PE=1 SV=2)

HSP 1 Score: 70.5 bits (171), Expect = 4.3e-11
Identity = 70/256 (27.34%), Postives = 114/256 (44.53%), Query Frame = 0

Query: 103 NSPKDGVISYEELSDWINGQAIERLNYRTTKQLEFYDKNGDNAISFHEYLPQF------T 162
           +S  DG ++  ELS WI             +Q   YDKN D  +++ EY  Q        
Sbjct: 77  DSDSDGFLTENELSQWIQMSFKHYAMQEAKQQFVEYDKNSDGTVTWDEYNVQMYDRVIDF 136

Query: 163 KEDIARNDTGYGEAGWW----IKQFTNADVDQNGLLHFDELKDFLHPEDSSNYRIQNWLL 222
            E+ A +DT             K+F  A+ D    L+ +E   F HPE+     +  +++
Sbjct: 137 DENTALDDTEEESFRQLHLKDKKRFEKANQDSGPGLNLEEFIAFEHPEEVD--YMTEFVI 196

Query: 223 AQKMKRMDHDRDGKLNFDEFLHHTYNIYKNYIEFETQGDD---IPTAEEKF-DELDLDED 282
            + ++  D + DG ++ +EFL        +Y    T  +D   I   +++F ++ D D D
Sbjct: 197 QEALEEHDKNGDGFVSLEEFL-------GDYRRDPTANEDPEWILVEKDRFVNDYDKDSD 256

Query: 283 EVLSVEEIRPLFQYLHPGEVSYAQHYTSHLINEADDNKDGYLTIDEMLNHEYAFYSTVYE 342
             L  +E   L  ++ P     AQ    HLI+E D N D  L+ +E+L ++  F ++   
Sbjct: 257 GRLDPQE---LLSWVVPNNQGIAQEEALHLIDEMDLNSDKKLSEEEILENQDLFLTSEAT 316

Query: 343 NQNGDYEDDY-YHDEL 344
           +      DDY YHDEL
Sbjct: 317 DYGRQLHDDYFYHDEL 320

BLAST of IVF0020638 vs. ExPASy TrEMBL
Match: A0A5D3CJD2 (Reticulocalbin-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold227G001480 PE=4 SV=1)

HSP 1 Score: 710.3 bits (1832), Expect = 3.9e-201
Identity = 343/343 (100.00%), Postives = 343/343 (100.00%), Query Frame = 0

Query: 1   MGKAVVYVLTATAFAVFFLISPSNFHNRNYQQPTRRLGFKFPNPTFDPLVTEMERLEAEE 60
           MGKAVVYVLTATAFAVFFLISPSNFHNRNYQQPTRRLGFKFPNPTFDPLVTEMERLEAEE
Sbjct: 1   MGKAVVYVLTATAFAVFFLISPSNFHNRNYQQPTRRLGFKFPNPTFDPLVTEMERLEAEE 60

Query: 61  RGENAIGMENQNHKITDAYRNYYDEGRLNISLRLLVLFPLLDNSPKDGVISYEELSDWIN 120
           RGENAIGMENQNHKITDAYRNYYDEGRLNISLRLLVLFPLLDNSPKDGVISYEELSDWIN
Sbjct: 61  RGENAIGMENQNHKITDAYRNYYDEGRLNISLRLLVLFPLLDNSPKDGVISYEELSDWIN 120

Query: 121 GQAIERLNYRTTKQLEFYDKNGDNAISFHEYLPQFTKEDIARNDTGYGEAGWWIKQFTNA 180
           GQAIERLNYRTTKQLEFYDKNGDNAISFHEYLPQFTKEDIARNDTGYGEAGWWIKQFTNA
Sbjct: 121 GQAIERLNYRTTKQLEFYDKNGDNAISFHEYLPQFTKEDIARNDTGYGEAGWWIKQFTNA 180

Query: 181 DVDQNGLLHFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDRDGKLNFDEFLHHTYNIY 240
           DVDQNGLLHFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDRDGKLNFDEFLHHTYNIY
Sbjct: 181 DVDQNGLLHFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDRDGKLNFDEFLHHTYNIY 240

Query: 241 KNYIEFETQGDDIPTAEEKFDELDLDEDEVLSVEEIRPLFQYLHPGEVSYAQHYTSHLIN 300
           KNYIEFETQGDDIPTAEEKFDELDLDEDEVLSVEEIRPLFQYLHPGEVSYAQHYTSHLIN
Sbjct: 241 KNYIEFETQGDDIPTAEEKFDELDLDEDEVLSVEEIRPLFQYLHPGEVSYAQHYTSHLIN 300

Query: 301 EADDNKDGYLTIDEMLNHEYAFYSTVYENQNGDYEDDYYHDEL 344
           EADDNKDGYLTIDEMLNHEYAFYSTVYENQNGDYEDDYYHDEL
Sbjct: 301 EADDNKDGYLTIDEMLNHEYAFYSTVYENQNGDYEDDYYHDEL 343

BLAST of IVF0020638 vs. ExPASy TrEMBL
Match: A0A5A7U5L5 (Reticulocalbin-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold175G001680 PE=4 SV=1)

HSP 1 Score: 702.2 bits (1811), Expect = 1.1e-198
Identity = 338/343 (98.54%), Postives = 343/343 (100.00%), Query Frame = 0

Query: 1   MGKAVVYVLTATAFAVFFLISPSNFHNRNYQQPTRRLGFKFPNPTFDPLVTEMERLEAEE 60
           MGKAVVYVLTATAFAVFFLISPSNFH+RNYQQPTRRLGFKFPNPTFDPLVTEMERLEAEE
Sbjct: 1   MGKAVVYVLTATAFAVFFLISPSNFHSRNYQQPTRRLGFKFPNPTFDPLVTEMERLEAEE 60

Query: 61  RGENAIGMENQNHKITDAYRNYYDEGRLNISLRLLVLFPLLDNSPKDGVISYEELSDWIN 120
           RGENAIG+ENQNHKITDAYRNYYD+GRLNISLRLLVLFPLLDNSPKDGVISYEELSDWIN
Sbjct: 61  RGENAIGVENQNHKITDAYRNYYDDGRLNISLRLLVLFPLLDNSPKDGVISYEELSDWIN 120

Query: 121 GQAIERLNYRTTKQLEFYDKNGDNAISFHEYLPQFTKEDIARNDTGYGEAGWWIKQFTNA 180
           GQAIERLNYRTTKQLEFYDKNGDNAISFHEYLPQFTKEDIARN+TGYGEAGWWIKQFTNA
Sbjct: 121 GQAIERLNYRTTKQLEFYDKNGDNAISFHEYLPQFTKEDIARNETGYGEAGWWIKQFTNA 180

Query: 181 DVDQNGLLHFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDRDGKLNFDEFLHHTYNIY 240
           DVDQNGLLHFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDRDGKLNFDEFLHHTY+IY
Sbjct: 181 DVDQNGLLHFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDRDGKLNFDEFLHHTYDIY 240

Query: 241 KNYIEFETQGDDIPTAEEKFDELDLDEDEVLSVEEIRPLFQYLHPGEVSYAQHYTSHLIN 300
           KNYIEFETQGDDIPTAEEKFDELDLDEDEVLSVEEIRPLFQYLHPGEVSYAQHYTSHLIN
Sbjct: 241 KNYIEFETQGDDIPTAEEKFDELDLDEDEVLSVEEIRPLFQYLHPGEVSYAQHYTSHLIN 300

Query: 301 EADDNKDGYLTIDEMLNHEYAFYSTVYENQNGDYEDDYYHDEL 344
           EADDNKDGYLTIDEMLNHEYAFYSTVYENQNGDYEDDYYHDEL
Sbjct: 301 EADDNKDGYLTIDEMLNHEYAFYSTVYENQNGDYEDDYYHDEL 343

BLAST of IVF0020638 vs. ExPASy TrEMBL
Match: A0A1S3C255 (reticulocalbin-2 OS=Cucumis melo OX=3656 GN=LOC103496051 PE=4 SV=1)

HSP 1 Score: 702.2 bits (1811), Expect = 1.1e-198
Identity = 338/343 (98.54%), Postives = 343/343 (100.00%), Query Frame = 0

Query: 1   MGKAVVYVLTATAFAVFFLISPSNFHNRNYQQPTRRLGFKFPNPTFDPLVTEMERLEAEE 60
           MGKAVVYVLTATAFAVFFLISPSNFH+RNYQQPTRRLGFKFPNPTFDPLVTEMERLEAEE
Sbjct: 1   MGKAVVYVLTATAFAVFFLISPSNFHSRNYQQPTRRLGFKFPNPTFDPLVTEMERLEAEE 60

Query: 61  RGENAIGMENQNHKITDAYRNYYDEGRLNISLRLLVLFPLLDNSPKDGVISYEELSDWIN 120
           RGENAIG+ENQNHKITDAYRNYYD+GRLNISLRLLVLFPLLDNSPKDGVISYEELSDWIN
Sbjct: 61  RGENAIGVENQNHKITDAYRNYYDDGRLNISLRLLVLFPLLDNSPKDGVISYEELSDWIN 120

Query: 121 GQAIERLNYRTTKQLEFYDKNGDNAISFHEYLPQFTKEDIARNDTGYGEAGWWIKQFTNA 180
           GQAIERLNYRTTKQLEFYDKNGDNAISFHEYLPQFTKEDIARN+TGYGEAGWWIKQFTNA
Sbjct: 121 GQAIERLNYRTTKQLEFYDKNGDNAISFHEYLPQFTKEDIARNETGYGEAGWWIKQFTNA 180

Query: 181 DVDQNGLLHFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDRDGKLNFDEFLHHTYNIY 240
           DVDQNGLLHFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDRDGKLNFDEFLHHTY+IY
Sbjct: 181 DVDQNGLLHFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDRDGKLNFDEFLHHTYDIY 240

Query: 241 KNYIEFETQGDDIPTAEEKFDELDLDEDEVLSVEEIRPLFQYLHPGEVSYAQHYTSHLIN 300
           KNYIEFETQGDDIPTAEEKFDELDLDEDEVLSVEEIRPLFQYLHPGEVSYAQHYTSHLIN
Sbjct: 241 KNYIEFETQGDDIPTAEEKFDELDLDEDEVLSVEEIRPLFQYLHPGEVSYAQHYTSHLIN 300

Query: 301 EADDNKDGYLTIDEMLNHEYAFYSTVYENQNGDYEDDYYHDEL 344
           EADDNKDGYLTIDEMLNHEYAFYSTVYENQNGDYEDDYYHDEL
Sbjct: 301 EADDNKDGYLTIDEMLNHEYAFYSTVYENQNGDYEDDYYHDEL 343

BLAST of IVF0020638 vs. ExPASy TrEMBL
Match: A0A0A0LAH3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G238180 PE=4 SV=1)

HSP 1 Score: 663.3 bits (1710), Expect = 5.5e-187
Identity = 318/343 (92.71%), Postives = 334/343 (97.38%), Query Frame = 0

Query: 1   MGKAVVYVLTATAFAVFFLISPSNFHNRNYQQPTRRLGFKFPNPTFDPLVTEMERLEAEE 60
           MGKAVVYVLTAT FA+FFLISPSNFHNR++QQ TRRLGFKFPNPTFDPLVTEMERL AEE
Sbjct: 1   MGKAVVYVLTATVFALFFLISPSNFHNRSHQQATRRLGFKFPNPTFDPLVTEMERLAAEE 60

Query: 61  RGENAIGMENQNHKITDAYRNYYDEGRLNISLRLLVLFPLLDNSPKDGVISYEELSDWIN 120
           RGENAIG++NQNHKI D+YRNYYDEGRLNISLRLLVLFPLLDNSPKDGVISYEELSDWIN
Sbjct: 61  RGENAIGVDNQNHKIIDSYRNYYDEGRLNISLRLLVLFPLLDNSPKDGVISYEELSDWIN 120

Query: 121 GQAIERLNYRTTKQLEFYDKNGDNAISFHEYLPQFTKEDIARNDTGYGEAGWWIKQFTNA 180
           GQAIERLNYRTTKQLEFYDKNGD+AISFHEYLPQFT+EDIARN+TGYGEAGWW KQFTNA
Sbjct: 121 GQAIERLNYRTTKQLEFYDKNGDDAISFHEYLPQFTEEDIARNETGYGEAGWWRKQFTNA 180

Query: 181 DVDQNGLLHFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDRDGKLNFDEFLHHTYNIY 240
           DVD NGLL+FDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHD+DGKLNFDEFLHHTY+IY
Sbjct: 181 DVDNNGLLYFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDKDGKLNFDEFLHHTYDIY 240

Query: 241 KNYIEFETQGDDIPTAEEKFDELDLDEDEVLSVEEIRPLFQYLHPGEVSYAQHYTSHLIN 300
           KNYIEFETQG+D+P+AEEKFDELDLDEDEVLS EE+RPLFQYLHPGEVSYAQHYTSHLIN
Sbjct: 241 KNYIEFETQGEDVPSAEEKFDELDLDEDEVLSTEELRPLFQYLHPGEVSYAQHYTSHLIN 300

Query: 301 EADDNKDGYLTIDEMLNHEYAFYSTVYENQNGDYEDDYYHDEL 344
           EADDNKDGYLTIDEMLNHEY FYSTVYENQNGDYEDD YHDEL
Sbjct: 301 EADDNKDGYLTIDEMLNHEYVFYSTVYENQNGDYEDD-YHDEL 342

BLAST of IVF0020638 vs. ExPASy TrEMBL
Match: A0A6J1CHQ2 (reticulocalbin-2 OS=Momordica charantia OX=3673 GN=LOC111011736 PE=4 SV=1)

HSP 1 Score: 538.9 bits (1387), Expect = 1.6e-149
Identity = 262/348 (75.29%), Postives = 301/348 (86.49%), Query Frame = 0

Query: 1   MGKAVVYVLTATAFAVFFLISPSNFHNRNYQQPTRRLGFKFPNPTFDPLVTEMERLEAEE 60
           MG+AVVY+   TAFA FF+  PSN  +RN+Q+ TRRLG+K   PTFDPLV EMER   E 
Sbjct: 1   MGRAVVYLFLITAFAFFFVFYPSNSPDRNHQELTRRLGYKLRAPTFDPLVEEMERYAEEH 60

Query: 61  ---RGENAIGMENQN--HKITDAYRNYYDEGRLNISLRLLVLFPLLDNSPKDGVISYEEL 120
              RG NAI  +     +++ DAY++YYDEGRL ISLRLLVLFPLLD+SPKDGVISYEEL
Sbjct: 61  GSGRGRNAISGQRTTTANQVPDAYKDYYDEGRLKISLRLLVLFPLLDHSPKDGVISYEEL 120

Query: 121 SDWINGQAIERLNYRTTKQLEFYDKNGDNAISFHEYLPQFTKEDIARNDTGYGEAGWWIK 180
           SDWI  QA++RLNYRT K+L+F+DKNGD AISF EYLPQFT+EDIA+  T +GEAGWW +
Sbjct: 121 SDWIIEQAVQRLNYRTRKELDFHDKNGDGAISFREYLPQFTEEDIAKRGTAHGEAGWWKR 180

Query: 181 QFTNADVDQNGLLHFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDRDGKLNFDEFLHH 240
           QFTNAD D NGLL+F+ELKDFLHPEDSSNYRIQNWLL +KMKRMD+DRDGKLNFDEFLHH
Sbjct: 181 QFTNADTDPNGLLNFEELKDFLHPEDSSNYRIQNWLLREKMKRMDYDRDGKLNFDEFLHH 240

Query: 241 TYNIYKNYIEFETQGDDIPTAEEKFDELDLDEDEVLSVEEIRPLFQYLHPGEVSYAQHYT 300
            ++IYK+YIEFETQGDD+PTAEEKFDELDLDEDE+LSVEE+RPLFQYLHPGE+SYAQHYT
Sbjct: 241 VFDIYKSYIEFETQGDDVPTAEEKFDELDLDEDELLSVEELRPLFQYLHPGELSYAQHYT 300

Query: 301 SHLINEADDNKDGYLTIDEMLNHEYAFYSTVYENQNGDYEDDYYHDEL 344
           SHLI EADDNKDGYL++DEMLNHEY FY++VYE +NGDYEDD YHDEL
Sbjct: 301 SHLIYEADDNKDGYLSLDEMLNHEYVFYNSVYEGRNGDYEDD-YHDEL 347

BLAST of IVF0020638 vs. NCBI nr
Match: TYK11288.1 (reticulocalbin-2 [Cucumis melo var. makuwa])

HSP 1 Score: 711 bits (1834), Expect = 1.09e-258
Identity = 343/343 (100.00%), Postives = 343/343 (100.00%), Query Frame = 0

Query: 1   MGKAVVYVLTATAFAVFFLISPSNFHNRNYQQPTRRLGFKFPNPTFDPLVTEMERLEAEE 60
           MGKAVVYVLTATAFAVFFLISPSNFHNRNYQQPTRRLGFKFPNPTFDPLVTEMERLEAEE
Sbjct: 1   MGKAVVYVLTATAFAVFFLISPSNFHNRNYQQPTRRLGFKFPNPTFDPLVTEMERLEAEE 60

Query: 61  RGENAIGMENQNHKITDAYRNYYDEGRLNISLRLLVLFPLLDNSPKDGVISYEELSDWIN 120
           RGENAIGMENQNHKITDAYRNYYDEGRLNISLRLLVLFPLLDNSPKDGVISYEELSDWIN
Sbjct: 61  RGENAIGMENQNHKITDAYRNYYDEGRLNISLRLLVLFPLLDNSPKDGVISYEELSDWIN 120

Query: 121 GQAIERLNYRTTKQLEFYDKNGDNAISFHEYLPQFTKEDIARNDTGYGEAGWWIKQFTNA 180
           GQAIERLNYRTTKQLEFYDKNGDNAISFHEYLPQFTKEDIARNDTGYGEAGWWIKQFTNA
Sbjct: 121 GQAIERLNYRTTKQLEFYDKNGDNAISFHEYLPQFTKEDIARNDTGYGEAGWWIKQFTNA 180

Query: 181 DVDQNGLLHFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDRDGKLNFDEFLHHTYNIY 240
           DVDQNGLLHFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDRDGKLNFDEFLHHTYNIY
Sbjct: 181 DVDQNGLLHFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDRDGKLNFDEFLHHTYNIY 240

Query: 241 KNYIEFETQGDDIPTAEEKFDELDLDEDEVLSVEEIRPLFQYLHPGEVSYAQHYTSHLIN 300
           KNYIEFETQGDDIPTAEEKFDELDLDEDEVLSVEEIRPLFQYLHPGEVSYAQHYTSHLIN
Sbjct: 241 KNYIEFETQGDDIPTAEEKFDELDLDEDEVLSVEEIRPLFQYLHPGEVSYAQHYTSHLIN 300

Query: 301 EADDNKDGYLTIDEMLNHEYAFYSTVYENQNGDYEDDYYHDEL 343
           EADDNKDGYLTIDEMLNHEYAFYSTVYENQNGDYEDDYYHDEL
Sbjct: 301 EADDNKDGYLTIDEMLNHEYAFYSTVYENQNGDYEDDYYHDEL 343

BLAST of IVF0020638 vs. NCBI nr
Match: XP_008455987.1 (PREDICTED: reticulocalbin-2 [Cucumis melo] >KAA0050524.1 reticulocalbin-2 [Cucumis melo var. makuwa])

HSP 1 Score: 702 bits (1813), Expect = 1.73e-255
Identity = 338/343 (98.54%), Postives = 343/343 (100.00%), Query Frame = 0

Query: 1   MGKAVVYVLTATAFAVFFLISPSNFHNRNYQQPTRRLGFKFPNPTFDPLVTEMERLEAEE 60
           MGKAVVYVLTATAFAVFFLISPSNFH+RNYQQPTRRLGFKFPNPTFDPLVTEMERLEAEE
Sbjct: 1   MGKAVVYVLTATAFAVFFLISPSNFHSRNYQQPTRRLGFKFPNPTFDPLVTEMERLEAEE 60

Query: 61  RGENAIGMENQNHKITDAYRNYYDEGRLNISLRLLVLFPLLDNSPKDGVISYEELSDWIN 120
           RGENAIG+ENQNHKITDAYRNYYD+GRLNISLRLLVLFPLLDNSPKDGVISYEELSDWIN
Sbjct: 61  RGENAIGVENQNHKITDAYRNYYDDGRLNISLRLLVLFPLLDNSPKDGVISYEELSDWIN 120

Query: 121 GQAIERLNYRTTKQLEFYDKNGDNAISFHEYLPQFTKEDIARNDTGYGEAGWWIKQFTNA 180
           GQAIERLNYRTTKQLEFYDKNGDNAISFHEYLPQFTKEDIARN+TGYGEAGWWIKQFTNA
Sbjct: 121 GQAIERLNYRTTKQLEFYDKNGDNAISFHEYLPQFTKEDIARNETGYGEAGWWIKQFTNA 180

Query: 181 DVDQNGLLHFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDRDGKLNFDEFLHHTYNIY 240
           DVDQNGLLHFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDRDGKLNFDEFLHHTY+IY
Sbjct: 181 DVDQNGLLHFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDRDGKLNFDEFLHHTYDIY 240

Query: 241 KNYIEFETQGDDIPTAEEKFDELDLDEDEVLSVEEIRPLFQYLHPGEVSYAQHYTSHLIN 300
           KNYIEFETQGDDIPTAEEKFDELDLDEDEVLSVEEIRPLFQYLHPGEVSYAQHYTSHLIN
Sbjct: 241 KNYIEFETQGDDIPTAEEKFDELDLDEDEVLSVEEIRPLFQYLHPGEVSYAQHYTSHLIN 300

Query: 301 EADDNKDGYLTIDEMLNHEYAFYSTVYENQNGDYEDDYYHDEL 343
           EADDNKDGYLTIDEMLNHEYAFYSTVYENQNGDYEDDYYHDEL
Sbjct: 301 EADDNKDGYLTIDEMLNHEYAFYSTVYENQNGDYEDDYYHDEL 343

BLAST of IVF0020638 vs. NCBI nr
Match: XP_011651314.1 (reticulocalbin-2 [Cucumis sativus] >KGN57652.1 hypothetical protein Csa_010748 [Cucumis sativus])

HSP 1 Score: 664 bits (1712), Expect = 4.17e-240
Identity = 318/343 (92.71%), Postives = 334/343 (97.38%), Query Frame = 0

Query: 1   MGKAVVYVLTATAFAVFFLISPSNFHNRNYQQPTRRLGFKFPNPTFDPLVTEMERLEAEE 60
           MGKAVVYVLTAT FA+FFLISPSNFHNR++QQ TRRLGFKFPNPTFDPLVTEMERL AEE
Sbjct: 1   MGKAVVYVLTATVFALFFLISPSNFHNRSHQQATRRLGFKFPNPTFDPLVTEMERLAAEE 60

Query: 61  RGENAIGMENQNHKITDAYRNYYDEGRLNISLRLLVLFPLLDNSPKDGVISYEELSDWIN 120
           RGENAIG++NQNHKI D+YRNYYDEGRLNISLRLLVLFPLLDNSPKDGVISYEELSDWIN
Sbjct: 61  RGENAIGVDNQNHKIIDSYRNYYDEGRLNISLRLLVLFPLLDNSPKDGVISYEELSDWIN 120

Query: 121 GQAIERLNYRTTKQLEFYDKNGDNAISFHEYLPQFTKEDIARNDTGYGEAGWWIKQFTNA 180
           GQAIERLNYRTTKQLEFYDKNGD+AISFHEYLPQFT+EDIARN+TGYGEAGWW KQFTNA
Sbjct: 121 GQAIERLNYRTTKQLEFYDKNGDDAISFHEYLPQFTEEDIARNETGYGEAGWWRKQFTNA 180

Query: 181 DVDQNGLLHFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDRDGKLNFDEFLHHTYNIY 240
           DVD NGLL+FDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHD+DGKLNFDEFLHHTY+IY
Sbjct: 181 DVDNNGLLYFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDKDGKLNFDEFLHHTYDIY 240

Query: 241 KNYIEFETQGDDIPTAEEKFDELDLDEDEVLSVEEIRPLFQYLHPGEVSYAQHYTSHLIN 300
           KNYIEFETQG+D+P+AEEKFDELDLDEDEVLS EE+RPLFQYLHPGEVSYAQHYTSHLIN
Sbjct: 241 KNYIEFETQGEDVPSAEEKFDELDLDEDEVLSTEELRPLFQYLHPGEVSYAQHYTSHLIN 300

Query: 301 EADDNKDGYLTIDEMLNHEYAFYSTVYENQNGDYEDDYYHDEL 343
           EADDNKDGYLTIDEMLNHEY FYSTVYENQNGDYEDD YHDEL
Sbjct: 301 EADDNKDGYLTIDEMLNHEYVFYSTVYENQNGDYEDD-YHDEL 342

BLAST of IVF0020638 vs. NCBI nr
Match: XP_038880351.1 (calumenin-B [Benincasa hispida])

HSP 1 Score: 552 bits (1422), Expect = 6.37e-196
Identity = 276/347 (79.54%), Postives = 301/347 (86.74%), Query Frame = 0

Query: 1   MGKAVVYVLTATAFAVFFLISPSNFHNRNYQQPTRRLGFKFPNPTFDPLVTEMERLEAEE 60
           MG+AV YVL +TAFA FFL SPSN  +  +Q+ +RRLGFKF  PTFDPL  EMERL AEE
Sbjct: 1   MGRAVAYVLISTAFAAFFLFSPSNLQDWKHQELSRRLGFKFRTPTFDPLFAEMERL-AEE 60

Query: 61  RGENAIGMENQN----HKITDAYRNYYDEGRLNISLRLLVLFPLLDNSPKDGVISYEELS 120
              NAIG+ENQN    +++ D  ++YYDEG LNISLRLLVLFPLLD+SPKDG ISYEELS
Sbjct: 61  ---NAIGVENQNSAKRNQVRDDCKHYYDEGSLNISLRLLVLFPLLDHSPKDGAISYEELS 120

Query: 121 DWINGQAIERLNYRTTKQLEFYDKNGDNAISFHEYLPQFTKEDIARNDTGYGEAGWWIKQ 180
           DWI GQAIERLNYRT KQL F DKNGD AISFHEYLPQFT+EDIARN+TGYGEAGWW KQ
Sbjct: 121 DWITGQAIERLNYRTGKQLAFNDKNGDGAISFHEYLPQFTEEDIARNETGYGEAGWWRKQ 180

Query: 181 FTNADVDQNGLLHFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDRDGKLNFDEFLHHT 240
           FTNADVDQN LL+FDELKDFLHPEDSSNYRIQNWLL QKM RMDHD DGKL+FDEFL   
Sbjct: 181 FTNADVDQNNLLNFDELKDFLHPEDSSNYRIQNWLLRQKMNRMDHDSDGKLSFDEFLRQA 240

Query: 241 YNIYKNYIEFETQGDDIPTAEEKFDELDLDEDEVLSVEEIRPLFQYLHPGEVSYAQHYTS 300
           Y IYK+YIEFE QG+DIP+AEEKFDELDLDEDEVLSVEE+RPLFQYLHPGEVSYAQHYTS
Sbjct: 241 YGIYKSYIEFEMQGEDIPSAEEKFDELDLDEDEVLSVEELRPLFQYLHPGEVSYAQHYTS 300

Query: 301 HLINEADDNKDGYLTIDEMLNHEYAFYSTVYENQNGDYEDDYYHDEL 343
           HLIN+ADDNKDGYLT+DEMLNH+  FYSTVYEN NG+ EDD YHDEL
Sbjct: 301 HLINKADDNKDGYLTLDEMLNHDNVFYSTVYENNNGESEDD-YHDEL 342

BLAST of IVF0020638 vs. NCBI nr
Match: XP_022141310.1 (reticulocalbin-2 [Momordica charantia])

HSP 1 Score: 539 bits (1389), Expect = 8.09e-191
Identity = 262/348 (75.29%), Postives = 301/348 (86.49%), Query Frame = 0

Query: 1   MGKAVVYVLTATAFAVFFLISPSNFHNRNYQQPTRRLGFKFPNPTFDPLVTEMERLEAEE 60
           MG+AVVY+   TAFA FF+  PSN  +RN+Q+ TRRLG+K   PTFDPLV EMER   E 
Sbjct: 1   MGRAVVYLFLITAFAFFFVFYPSNSPDRNHQELTRRLGYKLRAPTFDPLVEEMERYAEEH 60

Query: 61  ---RGENAIGMENQN--HKITDAYRNYYDEGRLNISLRLLVLFPLLDNSPKDGVISYEEL 120
              RG NAI  +     +++ DAY++YYDEGRL ISLRLLVLFPLLD+SPKDGVISYEEL
Sbjct: 61  GSGRGRNAISGQRTTTANQVPDAYKDYYDEGRLKISLRLLVLFPLLDHSPKDGVISYEEL 120

Query: 121 SDWINGQAIERLNYRTTKQLEFYDKNGDNAISFHEYLPQFTKEDIARNDTGYGEAGWWIK 180
           SDWI  QA++RLNYRT K+L+F+DKNGD AISF EYLPQFT+EDIA+  T +GEAGWW +
Sbjct: 121 SDWIIEQAVQRLNYRTRKELDFHDKNGDGAISFREYLPQFTEEDIAKRGTAHGEAGWWKR 180

Query: 181 QFTNADVDQNGLLHFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDRDGKLNFDEFLHH 240
           QFTNAD D NGLL+F+ELKDFLHPEDSSNYRIQNWLL +KMKRMD+DRDGKLNFDEFLHH
Sbjct: 181 QFTNADTDPNGLLNFEELKDFLHPEDSSNYRIQNWLLREKMKRMDYDRDGKLNFDEFLHH 240

Query: 241 TYNIYKNYIEFETQGDDIPTAEEKFDELDLDEDEVLSVEEIRPLFQYLHPGEVSYAQHYT 300
            ++IYK+YIEFETQGDD+PTAEEKFDELDLDEDE+LSVEE+RPLFQYLHPGE+SYAQHYT
Sbjct: 241 VFDIYKSYIEFETQGDDVPTAEEKFDELDLDEDELLSVEELRPLFQYLHPGELSYAQHYT 300

Query: 301 SHLINEADDNKDGYLTIDEMLNHEYAFYSTVYENQNGDYEDDYYHDEL 343
           SHLI EADDNKDGYL++DEMLNHEY FY++VYE +NGDYEDD YHDEL
Sbjct: 301 SHLIYEADDNKDGYLSLDEMLNHEYVFYNSVYEGRNGDYEDD-YHDEL 347

BLAST of IVF0020638 vs. TAIR 10
Match: AT4G27790.1 (Calcium-binding EF hand family protein )

HSP 1 Score: 295.4 bits (755), Expect = 5.8e-80
Identity = 157/348 (45.11%), Postives = 226/348 (64.94%), Query Frame = 0

Query: 1   MGKAVVY-VLTATAFAVFFLISPSNFHNRNYQ-QPTRRLGFKFPNPTFDPLVTEMERLEA 60
           M K VVY +LTAT   +  L        ++ +   TRR+G +   P FDPLVT +ERL  
Sbjct: 1   MAKVVVYTILTATIIFLILLAHKKQNQTQSIEGLITRRIGRRLEMPVFDPLVTRIERLSH 60

Query: 61  E-ERGENAIGMENQNHKITDAYRNYY-DEGRLNISLRLLVLFPLLDNSPKDGVISYEELS 120
           E E G   +  E    +  D +  Y+  E RLN ++R+  LFPLLD SP+DG +S +EL 
Sbjct: 61  EKEAGTKTV--EAAKEEKDDMFEEYFAQERRLNTTMRIKFLFPLLDASPRDGFVSLKELQ 120

Query: 121 DWINGQAIERLNYRTTKQLEFYDKNGDNAISFHEYLPQFTKEDIARNDTGYGEAGWWIKQ 180
            W+  Q  + + YRT K+LE  DK+ D  I+F EYLPQF+K+DI +N+ G+GEAGWW++Q
Sbjct: 121 TWMMQQTEDNMVYRTAKELELQDKDKDGVITFEEYLPQFSKQDIEKNEKGHGEAGWWMEQ 180

Query: 181 FTNADVDQNGLLHFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDRDGKLNFDEFLHHT 240
           F N+D D NG L  +E  +FLHPEDS N   Q W+L ++M  MD + DGKL + EF+ + 
Sbjct: 181 FKNSDFDHNGSLDIEEFNNFLHPEDSRNGDTQRWVLKERMTGMDTNGDGKLEYKEFVKNA 240

Query: 241 YNIYKNYIEFETQGDD-IPTAEEKFDELDLDEDEVLSVEEIRPLFQYLHPGEVSYAQHYT 300
           Y +YK + +FE + D+ +PT +  F E+D D+D  L  +E+RP+ QYL PGE+SYA+ Y+
Sbjct: 241 YEMYKEFAKFEKEEDENVPTPQLLFAEMDRDKDRFLVADELRPILQYLQPGEMSYAKFYS 300

Query: 301 SHLINEADDNKDGYLTIDEMLNHEYAFYSTVYENQNGDYEDDYYHDEL 344
           + L +EAD++KDG L+++EML+HE  FY  V+ +++ D ED + HDEL
Sbjct: 301 TFLCHEADEDKDGKLSLEEMLHHEDVFYKAVH-HEDLDDEDYFDHDEL 345

BLAST of IVF0020638 vs. TAIR 10
Match: AT5G08580.1 (Calcium-binding EF hand family protein )

HSP 1 Score: 194.5 bits (493), Expect = 1.4e-49
Identity = 131/393 (33.33%), Postives = 202/393 (51.40%), Query Frame = 0

Query: 1   MGKAVVYVLTATAFAVFFLISPS-------NFHNRNYQQPTR---RLGFKFPNPT----- 60
           M KA V +       V FL+S S       + H+  + Q  R   R  F F  PT     
Sbjct: 1   MSKASVILYITVGILVLFLVSYSPKKKGDHDHHHGGHNQHHRLKLRSSFNF-KPTRHDPV 60

Query: 61  -FDPLVTEMERLEAEER-------------------------GENAIGMENQN--HKITD 120
            FDPLV +MER   ++                           E+A G E+Q    +  D
Sbjct: 61  PFDPLVADMERRREDKEWERQYIEHSHPELVSHSQKETTGGGHEHAPGHESQPEWEEFMD 120

Query: 121 AYRNYYDEGRLNISLRLLVLFPLLDNSPKDGVISYEELSDWINGQAIERLNYRTTKQLEF 180
           A     DE + N++ RL++LFP +D SP DG ++  EL++W    + + + +RT + L+ 
Sbjct: 121 AEDYLNDEEKFNVTDRLILLFPKIDVSPADGFMTESELTEWTMQSSAKEVVHRTQRDLDV 180

Query: 181 YDKNGDNAISFHEYLPQFTKEDIARNDTGYGEAGWWIKQFTNA-DVDQNGLLHFDELKDF 240
           +D+N D  ISF EY P         N  GY + GWW ++  NA D + +GLL+  E  DF
Sbjct: 181 HDRNKDGFISFSEYEPPSWVRKSDNNSFGY-DMGWWKEEHFNASDANGDGLLNLTEFNDF 240

Query: 241 LHPEDSSNYRIQNWLLAQKMKRMDHDRDGKLNFDEFLHHTYNIYKNYIEFETQG----DD 300
           LHP D+ N ++  WL  ++++  D D+DGK++F+EF H  ++  +NY E          D
Sbjct: 241 LHPADTKNPKLLLWLCKEEVRERDSDKDGKISFEEFFHGLFDTVRNYEEDNHNSTHPYHD 300

Query: 301 IP--TAEEKFDELDLDEDEVLSVEEIRPLFQYLHPGEVSYAQHYTSHLINEADDNKDGYL 343
           +P   A++ F +LD ++D  LS  E+ P+   +HP E  YA+    ++I++AD +KD  L
Sbjct: 301 LPEGPAKQLFSQLDKNDDGYLSDVELLPIISKIHPTEHYYAKQQADYIISQADSDKDRRL 360

BLAST of IVF0020638 vs. TAIR 10
Match: AT1G74740.1 (calcium-dependent protein kinase 30 )

HSP 1 Score: 51.6 bits (122), Expect = 1.5e-06
Identity = 37/137 (27.01%), Postives = 63/137 (45.99%), Query Frame = 0

Query: 97  LFPLLDNSPKDGVISYEELSDWINGQAIERLNYRTTKQLEFYDKNGDNAISFHEYLPQFT 156
           +F L+D+   DG ISY EL   +     +         +E  D NG+  + + E++    
Sbjct: 368 MFTLMDDD-NDGKISYLELRAGLRKVGSQLGEPEIKLLMEVADVNGNGCLDYGEFVAVII 427

Query: 157 KEDIARNDTGYGEAGWWIKQFTNADVDQNGLLHFDELKDFLHPEDSSNYRIQNWLLAQKM 216
                 ND  + +A      F   D D +G +  +EL++ L  E        N ++   M
Sbjct: 428 HLQKMENDEHFRQA------FMFFDKDGSGYIESEELREALTDELGEP---DNSVIIDIM 487

Query: 217 KRMDHDRDGKLNFDEFL 234
           + +D D+DGK+N+DEF+
Sbjct: 488 REVDTDKDGKINYDEFV 494

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
J3S9D93.8e-1226.24Reticulocalbin-2 OS=Crotalus adamanteus OX=8729 PE=1 SV=1[more]
Q8BP923.8e-1228.13Reticulocalbin-2 OS=Mus musculus OX=10090 GN=Rcn2 PE=1 SV=1[more]
B5X4E01.5e-1126.25Calumenin-B OS=Salmo salar OX=8030 GN=calub PE=2 SV=1[more]
O934342.5e-1126.24Reticulocalbin-1 OS=Takifugu rubripes OX=31033 GN=rcn1 PE=3 SV=1[more]
Q627034.3e-1127.34Reticulocalbin-2 OS=Rattus norvegicus OX=10116 GN=Rcn2 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A5D3CJD23.9e-201100.00Reticulocalbin-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold227G001... [more]
A0A5A7U5L51.1e-19898.54Reticulocalbin-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold175G001... [more]
A0A1S3C2551.1e-19898.54reticulocalbin-2 OS=Cucumis melo OX=3656 GN=LOC103496051 PE=4 SV=1[more]
A0A0A0LAH35.5e-18792.71Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G238180 PE=4 SV=1[more]
A0A6J1CHQ21.6e-14975.29reticulocalbin-2 OS=Momordica charantia OX=3673 GN=LOC111011736 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
TYK11288.11.09e-258100.00reticulocalbin-2 [Cucumis melo var. makuwa][more]
XP_008455987.11.73e-25598.54PREDICTED: reticulocalbin-2 [Cucumis melo] >KAA0050524.1 reticulocalbin-2 [Cucum... [more]
XP_011651314.14.17e-24092.71reticulocalbin-2 [Cucumis sativus] >KGN57652.1 hypothetical protein Csa_010748 [... [more]
XP_038880351.16.37e-19679.54calumenin-B [Benincasa hispida][more]
XP_022141310.18.09e-19175.29reticulocalbin-2 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT4G27790.15.8e-8045.11Calcium-binding EF hand family protein [more]
AT5G08580.11.4e-4933.33Calcium-binding EF hand family protein [more]
AT1G74740.11.5e-0627.01calcium-dependent protein kinase 30 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 211..239
e-value: 2.3
score: 14.8
coord: 172..200
e-value: 4.5
score: 12.5
coord: 294..322
e-value: 42.0
score: 4.6
coord: 130..158
e-value: 59.0
score: 3.5
coord: 255..283
e-value: 60.0
score: 3.4
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 257..317
e-value: 5.1E-7
score: 30.0
IPR002048EF-hand domainPFAMPF13202EF-hand_5coord: 216..232
e-value: 0.014
score: 14.9
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 251..286
score: 9.408546
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 212..242
score: 8.432208
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 168..203
score: 8.962221
NoneNo IPR availableGENE3D1.10.238.10coord: 43..165
e-value: 1.2E-6
score: 30.5
coord: 236..320
e-value: 2.3E-9
score: 39.3
NoneNo IPR availableGENE3D1.10.238.10coord: 175..235
e-value: 1.5E-9
score: 39.8
NoneNo IPR availablePANTHERPTHR10827:SF77CALCIUM-BINDING EF HAND FAMILY PROTEINcoord: 1..343
NoneNo IPR availablePANTHERPTHR10827RETICULOCALBINcoord: 1..343
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 139..151
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 303..315
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 220..232
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 170..334
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 94..156

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0020638.1IVF0020638.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005509 calcium ion binding