IVF0014656 (gene) Melon (IVF77) v1

Overview
NameIVF0014656
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionRNA pseudouridine synthase 1
Locationchr05: 22807312 .. 22809801 (+)
RNA-Seq ExpressionIVF0014656
SyntenyIVF0014656
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCCTCTTCATCTCACTTCATCCCAATTTCTCAATTTTCCCCAAAACCTTCGCCAAATTCCCATTCACAAAACCCTTCTGTTTCCACAGATTCAAATCCGTCAGCAATAGCATGACGGACTCATCGGAACCGGTGACCGGAAACTGCCCATCAACCGGAGAGAATTACCCGGTCCCCTTATCGCCTCCACTGCCGGCCATATCCAAGAACTTAGAGCTCGCCAGAGCCATGGCTGCCTCCTCTAAATCCAGCCTCTTTGCTTTATCGGCAAACGATGTTATATACGAAGACGAGTGGCTCATTGCCGTCAATAAACCTCAAGGAATCTACTGTGAAAATGTTTTGGCTGCTGTTCCTCGTCTTCTTTGTGATTCGGCTAACGCTGGTTTGTGAATCTTACTGTGAAAGCGTTTTTTTTTCAGACGAATTCAACTTTTTACTACATTTTGGATTTTCTAATCGAATGGTAATGGTGGGTTTGCAACTTTTAACTTGCTTTCGTTTGACGATTTGTGGAAAGGGTATGTATAATCATGGACTTCCCGTGGATAACGAAAGATGTTAGAACTGAGTTTTCGTTAAAATTGACGGGTTTTGAAAGATTTTGTTCCATATAGGTCATTTGCATTGATTTTCTAGATCAAACTTTTGTATATTGATAATGTAACTTGGTTTTTGTTGAAAGTTAGCTGAAAGTAATGGGCGAATGCGATGAAAGTTTAACAGAAGTTTAAAATTGTAATGCTTATCTATGTGTACGGTCCAATTTGTTGAAAATTTGAAGTTTGGAGTAATAATTGATTCGTATTGCCAAGCTTTTGGGTAGGATTTGATCTTTCATCAAATTTGTTTAGAACGAGTGATTTTCTATTGATTTAAGCTGATTAAAAATGCAATTCTCAAGTTCTATTTCCTCATCTGTAACTATCTTCTTGTAATTATTATGTAGGTGTTATTTTGCATAGTTGGAGTCTCTTCATATAGGGAGAGGCGCCTTTTTTCTGGGCTGTGCTTTTATTTTTATGCCGGTGTATTTTTTCTTTTTTTTTCCAATAAAGGCCGGTGTTTCTAAAAGAAAATAATACGTTTACAGAAGAAGCAAGGGCGAGTAACTGATTTCTGATTCTTTTCCTACAATCTACATAGCCTAGCAATGTTGCTGATTGTTAGATAGTTGATATAAATGTTTTAGATGTATCCTGGTTGTTGAAGAGAGAAGGGTGCTTGAATGTTTCTGAAGTGTTTTAACGATTGTCCAAAACAATGCCATGTAATTACATCTTTTGCTAATTCAATCATTATCAATTTGTTGGGAGTGTTCTTAGCTCGAATTCTTCATATGCAGGGATCAAAACCAATTTGCCAGAGCTTCATCTTGCTAACCGCCTTGATCGTGACACAAGTGGGGTTATGGTTATAACGAAGTCACATAAAGTTGCTTCTAAATTAGTGAAGGCATTTACCGACCACACGGTTTCAAAATCATATATCGCCTTCTGTGTTGGCACATCTCCAAAATGGAAAAAAATCCACGTCAAATCTGGTCATGGAAGGTCAAAGTTTGGGGTCTGGCGTGTCTATGCTGCAGCTGATGTGGGTCGTTCCTTACCTGGCGGTTCCATAGTCAGAGATATGGAAACATATTTTGAAGTACTGTCTGTAAATGGAAAAAACACCATGGAGGAATTACAAAAATTCAGGAAAGAGGAAGAAGAAACTATTGTAGTCCATACAAAATCTTTGGTGGACATTGATAGTCAGAAGGACGAGGTCTTGATAAGAGCAAGACCTCGCAGTGGAAGAACTCATCAAATCCGACTGCACTGCCAATATCTTGGAATTCCTATAAGAGGGGATGTGAAATATGAGGGTGTTACAGAGTGGAATGGGAAAACTTACGATAGCCATGAGCTTCATGCTGAAAGCTTGTACTTTGTGCATCCTATTACAGGTATTCCTCTCAAACTACAAGCTCCTTTACCATCATGGGCCAGCCAAGCGTTGCAGCCCCAACAACAAGAAGTGAATTCTCCACAGTCCTTCAAAACTATGCCTTAAGAAAATCATTGGCGCAATACAGTTAAGTGAGAACCAAATATATCCCTTATTATACAAGGAGATTCGGTGTTGAAGAATGTCGCTGAAGCAAAATGGAGCGATCTTCAATCAAATTCATAAAGTGCGACTGCAGACAGTTTTCGTACTGTCAAGTTGATGTCTGCAATTTTTCATCTATTGAATGAGCTCTTGTTTGCACGACAGTGTCTACAAAAATATCAGCAGTCGCAAACCAAGAAACCATCCGAGTTCTTGAAAATTTGAGCTCGAAATGGATTCTGGAAATGACCGAAGAATCATGTGTGTCAAAGGCTGATAAATTTGTGCAAAGCCCTTTGGAAATGGTTCTTCTGCAGTGGTTTTATCGATAAAATTGATGTACGGTGCTAAAACAGCCAAATTCTGTGGTTGGCATGTTGATTTATATTG

mRNA sequence

ATGGCCCTCTTCATCTCACTTCATCCCAATTTCTCAATTTTCCCCAAAACCTTCGCCAAATTCCCATTCACAAAACCCTTCTGTTTCCACAGATTCAAATCCGTCAGCAATAGCATGACGGACTCATCGGAACCGGTGACCGGAAACTGCCCATCAACCGGAGAGAATTACCCGGTCCCCTTATCGCCTCCACTGCCGGCCATATCCAAGAACTTAGAGCTCGCCAGAGCCATGGCTGCCTCCTCTAAATCCAGCCTCTTTGCTTTATCGGCAAACGATGTTATATACGAAGACGAGTGGCTCATTGCCGTCAATAAACCTCAAGGAATCTACTGTGAAAATGTTTTGGCTGCTGTTCCTCGTCTTCTTTGTGATTCGGCTAACGCTGGGATCAAAACCAATTTGCCAGAGCTTCATCTTGCTAACCGCCTTGATCGTGACACAAGTGGGGTTATGGTTATAACGAAGTCACATAAAGTTGCTTCTAAATTAGTGAAGGCATTTACCGACCACACGGTTTCAAAATCATATATCGCCTTCTGTGTTGGCACATCTCCAAAATGGAAAAAAATCCACGTCAAATCTGGTCATGGAAGGTCAAAGTTTGGGGTCTGGCGTGTCTATGCTGCAGCTGATGTGGGTCGTTCCTTACCTGGCGGTTCCATAGTCAGAGATATGGAAACATATTTTGAAGTACTGTCTGTAAATGGAAAAAACACCATGGAGGAATTACAAAAATTCAGGAAAGAGGAAGAAGAAACTATTGTAGTCCATACAAAATCTTTGGTGGACATTGATAGTCAGAAGGACGAGGTCTTGATAAGAGCAAGACCTCGCAGTGGAAGAACTCATCAAATCCGACTGCACTGCCAATATCTTGGAATTCCTATAAGAGGGGATGTGAAATATGAGGGTGTTACAGAGTGGAATGGGAAAACTTACGATAGCCATGAGCTTCATGCTGAAAGCTTGTACTTTGTGCATCCTATTACAGGTATTCCTCTCAAACTACAAGCTCCTTTACCATCATGGGCCAGCCAAGCGTTGCAGCCCCAACAACAAGAAGTGAATTCTCCACAGTCCTTCAAAACTATGCCTTAAGAAAATCATTGGCGCAATACAGTTAAGTGAGAACCAAATATATCCCTTATTATACAAGGAGATTCGGTGTTGAAGAATGTCGCTGAAGCAAAATGGAGCGATCTTCAATCAAATTCATAAAGTGCGACTGCAGACAGTTTTCGTACTGTCAAGTTGATGTCTGCAATTTTTCATCTATTGAATGAGCTCTTGTTTGCACGACAGTGTCTACAAAAATATCAGCAGTCGCAAACCAAGAAACCATCCGAGTTCTTGAAAATTTGAGCTCGAAATGGATTCTGGAAATGACCGAAGAATCATGTGTGTCAAAGGCTGATAAATTTGTGCAAAGCCCTTTGGAAATGGTTCTTCTGCAGTGGTTTTATCGATAAAATTGATGTACGGTGCTAAAACAGCCAAATTCTGTGGTTGGCATGTTGATTTATATTG

Coding sequence (CDS)

ATGGCCCTCTTCATCTCACTTCATCCCAATTTCTCAATTTTCCCCAAAACCTTCGCCAAATTCCCATTCACAAAACCCTTCTGTTTCCACAGATTCAAATCCGTCAGCAATAGCATGACGGACTCATCGGAACCGGTGACCGGAAACTGCCCATCAACCGGAGAGAATTACCCGGTCCCCTTATCGCCTCCACTGCCGGCCATATCCAAGAACTTAGAGCTCGCCAGAGCCATGGCTGCCTCCTCTAAATCCAGCCTCTTTGCTTTATCGGCAAACGATGTTATATACGAAGACGAGTGGCTCATTGCCGTCAATAAACCTCAAGGAATCTACTGTGAAAATGTTTTGGCTGCTGTTCCTCGTCTTCTTTGTGATTCGGCTAACGCTGGGATCAAAACCAATTTGCCAGAGCTTCATCTTGCTAACCGCCTTGATCGTGACACAAGTGGGGTTATGGTTATAACGAAGTCACATAAAGTTGCTTCTAAATTAGTGAAGGCATTTACCGACCACACGGTTTCAAAATCATATATCGCCTTCTGTGTTGGCACATCTCCAAAATGGAAAAAAATCCACGTCAAATCTGGTCATGGAAGGTCAAAGTTTGGGGTCTGGCGTGTCTATGCTGCAGCTGATGTGGGTCGTTCCTTACCTGGCGGTTCCATAGTCAGAGATATGGAAACATATTTTGAAGTACTGTCTGTAAATGGAAAAAACACCATGGAGGAATTACAAAAATTCAGGAAAGAGGAAGAAGAAACTATTGTAGTCCATACAAAATCTTTGGTGGACATTGATAGTCAGAAGGACGAGGTCTTGATAAGAGCAAGACCTCGCAGTGGAAGAACTCATCAAATCCGACTGCACTGCCAATATCTTGGAATTCCTATAAGAGGGGATGTGAAATATGAGGGTGTTACAGAGTGGAATGGGAAAACTTACGATAGCCATGAGCTTCATGCTGAAAGCTTGTACTTTGTGCATCCTATTACAGGTATTCCTCTCAAACTACAAGCTCCTTTACCATCATGGGCCAGCCAAGCGTTGCAGCCCCAACAACAAGAAGTGAATTCTCCACAGTCCTTCAAAACTATGCCTTAA

Protein sequence

MALFISLHPNFSIFPKTFAKFPFTKPFCFHRFKSVSNSMTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCENVLAAVPRLLCDSANAGIKTNLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQAPLPSWASQALQPQQQEVNSPQSFKTMP
Homology
BLAST of IVF0014656 vs. ExPASy Swiss-Prot
Match: Q2QNM3 (RNA pseudouridine synthase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g0560500 PE=2 SV=1)

HSP 1 Score: 371.3 bits (952), Expect = 1.2e-101
Identity = 183/300 (61.00%), Postives = 226/300 (75.33%), Query Frame = 0

Query: 52  STGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIY 111
           +TGE YPVP+SPP PA SK++EL RAM AS++S+  A S+  V++EDEWL  V+KP G+Y
Sbjct: 47  ATGE-YPVPVSPPYPAASKDVELRRAMTASARSA--AYSSAPVVFEDEWLAVVDKPAGVY 106

Query: 112 CENVLAAVPRLLCDSANAGIKTNLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDH 171
           C+ +L+A+P   C +A  G +   P LHLANRLDRDTSG+MVITK +KVA KLVKAFT+H
Sbjct: 107 CDALLSALP---CSAATLGDEATKPNLHLANRLDRDTSGLMVITKCNKVAGKLVKAFTEH 166

Query: 172 TVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDMETYFE 231
            V K+Y+A C+G  P W+KI + SGHGRSK G WRVYA +DVGRSLPGGS+VRDM T FE
Sbjct: 167 KVKKTYLALCIGYPPAWEKIKICSGHGRSKHGAWRVYAMSDVGRSLPGGSVVRDMSTRFE 226

Query: 232 VLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDE----VLIRARPRSGRTHQIR 291
           VL +NGK    E   F  +E E+I V  K+  D+ S  DE    +L+RA P+SGRTHQIR
Sbjct: 227 VLGINGKGQFREPSNFEVDETESITVQEKA-ADLTSDGDEKNSIILVRAYPQSGRTHQIR 286

Query: 292 LHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQAPLPSWASQ 348
           LHCQYLG PIRGDVKY GV EWNG  YD H LHAESL FVHP+TG+P+  ++PLPSWA++
Sbjct: 287 LHCQYLGFPIRGDVKYSGVIEWNGVDYDGHALHAESLSFVHPVTGLPVTFRSPLPSWANE 339

BLAST of IVF0014656 vs. ExPASy Swiss-Prot
Match: Q7XA65 (RNA pseudouridine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g56345 PE=2 SV=1)

HSP 1 Score: 361.3 bits (926), Expect = 1.3e-98
Identity = 181/294 (61.56%), Postives = 223/294 (75.85%), Query Frame = 0

Query: 56  NYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCENV 115
           NYP P+S P P ISK++EL RAM ASSKSSLF L+ +D++YEDE+L+AVNKP+G+YCE V
Sbjct: 39  NYPKPISAPPPPISKDIELRRAMEASSKSSLFNLTRDDILYEDEYLMAVNKPKGVYCEAV 98

Query: 116 LAAVPRLLCDSANAGIKTNLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSK 175
           L + P+++ DS++        E HLANRLDRDTSGVM+ITKSHKVA+KLVKAFT+H + K
Sbjct: 99  LRSAPQIVLDSSS--------EYHLANRLDRDTSGVMIITKSHKVAAKLVKAFTEHKIRK 158

Query: 176 SYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDMETYFEVLSV 235
           SYIA C+G+SP W+++ V SGHGRSK G WRVYAA DVGR LPGGS VRDMET FEV+SV
Sbjct: 159 SYIALCIGSSPNWRRVTVSSGHGRSKHGAWRVYAALDVGRVLPGGSFVRDMETTFEVVSV 218

Query: 236 NG-KNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDE--VLIRARPRSGRTHQIRLHCQY 295
           N  KN   EL+    +    IV   +  +      D+  V++RA PRSGRTHQIRLHCQY
Sbjct: 219 NSVKNESCELE----DVNHVIVAEGERELSCGGDDDDVVVVVRAFPRSGRTHQIRLHCQY 278

Query: 296 LGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQAPLPSWAS 347
           LGIPIRGDVKY GV EWNG+T++ HELHAE L   HP+TG  + ++APLP WA+
Sbjct: 279 LGIPIRGDVKYHGVYEWNGRTFEGHELHAECLSLDHPVTGDSIVIRAPLPYWAA 320

BLAST of IVF0014656 vs. ExPASy Swiss-Prot
Match: Q68XB2 (Ribosomal large subunit pseudouridine synthase C OS=Rickettsia typhi (strain ATCC VR-144 / Wilmington) OX=257363 GN=rluC PE=3 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 2.9e-18
Identity = 80/256 (31.25%), Postives = 117/256 (45.70%), Query Frame = 0

Query: 94  VIYEDEWLIAVNKPQGIYCENVLAAVPRLLCDSANAGIKTNLPELHLANRLDRDTSGVMV 153
           +IYED+ LIA+NKP  +  +    +   L  DSA   +     +  L +RLD++TSG+++
Sbjct: 91  LIYEDDHLIAINKPASLATQG--GSKINLSIDSALKYLNYQGADFKLVHRLDKETSGLLL 150

Query: 154 ITKSHKVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADV 213
           I K++  + KL  AF +  V K Y A   G   K   I VKS  G+SK    R++   D+
Sbjct: 151 IAKNYLSSVKLHDAFKEKLVIKKYFAITYGRPVKNVGI-VKSNIGKSK---GRMFKITDI 210

Query: 214 GRSLPGGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVL 273
                               S NGK                 + + K L  +++  +  L
Sbjct: 211 D-------------------SDNGK---------------LAITYYKLLKSLNN--NLFL 270

Query: 274 IRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGI 333
           I   P +GR HQ+RLH Q LG PI GD KY          Y    LHA ++Y    I G 
Sbjct: 271 IEFMPVTGRMHQLRLHAQLLGCPILGDDKYGNKEVMPYSKY--MFLHANNIYLSESIVGK 302

Query: 334 PLKLQAPLPSWASQAL 350
            +KL+A LP + ++ L
Sbjct: 331 EIKLEAKLPFYFTRRL 302

BLAST of IVF0014656 vs. ExPASy Swiss-Prot
Match: Q87S65 (Ribosomal large subunit pseudouridine synthase D OS=Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633) OX=223926 GN=rluD PE=3 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 4.2e-17
Identity = 81/292 (27.74%), Postives = 126/292 (43.15%), Query Frame = 0

Query: 93  DVIYEDEWLIAVNKPQ--------GIYCENVLAAVPRLLCDSANAGIKTNLPELHLANRL 152
           D++YED+ +I +NKP+        G     VL A+     D A       +P   + +RL
Sbjct: 85  DIVYEDDDIIVINKPRDFVVHPGAGTPDGTVLNALLHHYPDIA------EVPRAGIVHRL 144

Query: 153 DRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGV 212
           D+DT+G+MV+ K+    ++LV+A     +++ Y A  +G      K+    G   +K   
Sbjct: 145 DKDTTGLMVVAKTVPAQTRLVRALQKRNITREYEAIAIGRMTAGGKVDQPIGRHSTK--- 204

Query: 213 WRVYAAADVGRSLPGGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVD 272
             + A A +G         +   T++ V            + FR+        HT+    
Sbjct: 205 RTLMAVAPLG---------KPAVTHYRV-----------AEHFRE--------HTR---- 264

Query: 273 IDSQKDEVLIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEG-----------VTEWNGKT 332
                    IR R  +GRTHQIR+H  YL  P+ GD  Y G           +T+   + 
Sbjct: 265 ---------IRLRLETGRTHQIRVHMSYLQHPLLGDTAYGGRARIPTGASQELTDMI-RG 324

Query: 333 YDSHELHAESLYFVHPITGIPLKLQAPLPS---WASQALQPQQQEVNSPQSF 363
           +D   LHA  L F HPITG  L+  AP+P      ++AL+   +E   P  F
Sbjct: 325 FDRQALHAVMLRFEHPITGEELEFHAPVPDDMVAMTEALRKDTEEYGLPDEF 325

BLAST of IVF0014656 vs. ExPASy Swiss-Prot
Match: Q9ZDR7 (Ribosomal large subunit pseudouridine synthase C OS=Rickettsia prowazekii (strain Madrid E) OX=272947 GN=rluC PE=3 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 3.6e-16
Identity = 72/256 (28.12%), Postives = 113/256 (44.14%), Query Frame = 0

Query: 94  VIYEDEWLIAVNKPQGIYCENVLAAVPRLLCDSANAGIKTNLPELHLANRLDRDTSGVMV 153
           +IYED+ LIA+NKP  +  +    +   L  DSA   +     +  L +RLD++TSG+++
Sbjct: 91  LIYEDDHLIAINKPASLATQG--GSKINLSIDSALKYLNYQGADFKLVHRLDKETSGLLL 150

Query: 154 ITKSHKVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADV 213
           I K++  + KL  AF +  V K Y A   G  P      VKS  G+S             
Sbjct: 151 IAKNYLSSVKLHNAFKEKLVIKKYFAITYG-RPVKNVGTVKSNIGKS------------- 210

Query: 214 GRSLPGGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVL 273
                                   K  + ++     ++ +  + + K L  +++  +  L
Sbjct: 211 ------------------------KRRLSKIANIDSDDGKLAITYYKLLKSLNN--NLFL 270

Query: 274 IRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGI 333
           I   P +GR HQ+RLH Q LG PI GD KY          Y    LHA ++Y    I G 
Sbjct: 271 IEFMPVTGRMHQLRLHAQLLGCPILGDDKYGNKEIMPYSKY--MFLHANNIYLSETIVGK 302

Query: 334 PLKLQAPLPSWASQAL 350
            +KL+A LP + ++ +
Sbjct: 331 EIKLEAKLPFYFTRRI 302

BLAST of IVF0014656 vs. ExPASy TrEMBL
Match: A0A0A0LJY6 (PseudoU_synth_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G033960 PE=4 SV=1)

HSP 1 Score: 699.9 bits (1805), Expect = 5.6e-198
Identity = 347/366 (94.81%), Postives = 355/366 (96.99%), Query Frame = 0

Query: 1   MALFISLHPNFSIFPKTFAKFPFTKPFCFHRFKSVSNSMTDSSEPVTGNCPSTGENYPVP 60
           MALFISLHPNFSI PKTF +FPFTK FCFHRFKSVSNSMTDSSEPVTGNCPST ENYPVP
Sbjct: 1   MALFISLHPNFSISPKTFTEFPFTKSFCFHRFKSVSNSMTDSSEPVTGNCPSTAENYPVP 60

Query: 61  LSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCENVLAAVP 120
           LSPPLPAISKNLELARAM ASSKSSL+ALSANDVIYEDEWLIAVNKPQGIYCENVLAAVP
Sbjct: 61  LSPPLPAISKNLELARAMVASSKSSLYALSANDVIYEDEWLIAVNKPQGIYCENVLAAVP 120

Query: 121 RLLCDSANAGIKTNLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAF 180
           RLL DSANAGIKT+LPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAF
Sbjct: 121 RLLGDSANAGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAF 180

Query: 181 CVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDMETYFEVLSVNGKNT 240
           CVGTSPKWKKI+VKSGHGRSKFGVWRVYAAADVGRSLPGGS+VRDMETYFEVLSVNGKNT
Sbjct: 181 CVGTSPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGKNT 240

Query: 241 MEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARPRSGRTHQIRLHCQYLGIPIRGD 300
           MEELQKFR+ EEETIVVHTKSLVDIDS KDE+LIRARPRSGRTHQIRLHCQYLGIPIRGD
Sbjct: 241 MEELQKFRRGEEETIVVHTKSLVDIDSHKDEILIRARPRSGRTHQIRLHCQYLGIPIRGD 300

Query: 301 VKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQAPLPSWASQALQPQQQEVNSPQ 360
           VKYEGVTEWN K YDSHELHAESLYFVHP+TGIPLKLQAPLPSWASQALQPQQ EVNSPQ
Sbjct: 301 VKYEGVTEWNEKIYDSHELHAESLYFVHPVTGIPLKLQAPLPSWASQALQPQQHEVNSPQ 360

Query: 361 SFKTMP 367
           SFKTMP
Sbjct: 361 SFKTMP 366

BLAST of IVF0014656 vs. ExPASy TrEMBL
Match: A0A5A7TX25 (RNA pseudouridine synthase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G001190 PE=4 SV=1)

HSP 1 Score: 660.2 bits (1702), Expect = 5.0e-186
Identity = 328/328 (100.00%), Postives = 328/328 (100.00%), Query Frame = 0

Query: 39  MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 98
           MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED
Sbjct: 1   MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 60

Query: 99  EWLIAVNKPQGIYCENVLAAVPRLLCDSANAGIKTNLPELHLANRLDRDTSGVMVITKSH 158
           EWLIAVNKPQGIYCENVLAAVPRLLCDSANAGIKTNLPELHLANRLDRDTSGVMVITKSH
Sbjct: 61  EWLIAVNKPQGIYCENVLAAVPRLLCDSANAGIKTNLPELHLANRLDRDTSGVMVITKSH 120

Query: 159 KVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLP 218
           KVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLP
Sbjct: 121 KVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLP 180

Query: 219 GGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARP 278
           GGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARP
Sbjct: 181 GGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARP 240

Query: 279 RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQ 338
           RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQ
Sbjct: 241 RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQ 300

Query: 339 APLPSWASQALQPQQQEVNSPQSFKTMP 367
           APLPSWASQALQPQQQEVNSPQSFKTMP
Sbjct: 301 APLPSWASQALQPQQQEVNSPQSFKTMP 328

BLAST of IVF0014656 vs. ExPASy TrEMBL
Match: A0A1S3BW87 (RNA pseudouridine synthase 1 OS=Cucumis melo OX=3656 GN=LOC103494301 PE=4 SV=1)

HSP 1 Score: 660.2 bits (1702), Expect = 5.0e-186
Identity = 328/328 (100.00%), Postives = 328/328 (100.00%), Query Frame = 0

Query: 39  MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 98
           MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED
Sbjct: 1   MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 60

Query: 99  EWLIAVNKPQGIYCENVLAAVPRLLCDSANAGIKTNLPELHLANRLDRDTSGVMVITKSH 158
           EWLIAVNKPQGIYCENVLAAVPRLLCDSANAGIKTNLPELHLANRLDRDTSGVMVITKSH
Sbjct: 61  EWLIAVNKPQGIYCENVLAAVPRLLCDSANAGIKTNLPELHLANRLDRDTSGVMVITKSH 120

Query: 159 KVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLP 218
           KVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLP
Sbjct: 121 KVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLP 180

Query: 219 GGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARP 278
           GGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARP
Sbjct: 181 GGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARP 240

Query: 279 RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQ 338
           RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQ
Sbjct: 241 RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQ 300

Query: 339 APLPSWASQALQPQQQEVNSPQSFKTMP 367
           APLPSWASQALQPQQQEVNSPQSFKTMP
Sbjct: 301 APLPSWASQALQPQQQEVNSPQSFKTMP 328

BLAST of IVF0014656 vs. ExPASy TrEMBL
Match: A0A6J1DQ12 (RNA pseudouridine synthase 1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111023261 PE=4 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 6.3e-157
Identity = 279/330 (84.55%), Postives = 305/330 (92.42%), Query Frame = 0

Query: 39  MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 98
           M D S PVTGN P+TG+NYP+PLSPPLPAISKNLELARAMAASSKSSLFALSA DVI+ED
Sbjct: 1   MADPSIPVTGNSPATGQNYPIPLSPPLPAISKNLELARAMAASSKSSLFALSATDVIFED 60

Query: 99  EWLIAVNKPQGIYCENVLAAVPRLLCDSANA-----GIKTNLPELHLANRLDRDTSGVMV 158
           EWLIAVNKPQG+YCE+VLA+VPRLL DSA A     GI+ +LPELHLANRLDRDTSGVMV
Sbjct: 61  EWLIAVNKPQGVYCESVLASVPRLLGDSAMAGEQSGGIQISLPELHLANRLDRDTSGVMV 120

Query: 159 ITKSHKVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADV 218
           ITKSHKVASKLVKAFTDH VSKSYIAFCVG++PKWKKI+VKSGHGRSKFGVWRVYAAADV
Sbjct: 121 ITKSHKVASKLVKAFTDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADV 180

Query: 219 GRSLPGGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVL 278
           GRSLPGGS+VRDMETYFEVLSVNG++TM+EL + RK+EEETIVV +KSL+DID+ KDE+L
Sbjct: 181 GRSLPGGSVVRDMETYFEVLSVNGQSTMDELPEVRKDEEETIVVQSKSLIDIDAHKDEIL 240

Query: 279 IRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGI 338
           IRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGV EWNG  YDSHELHAESL+FVHP+TGI
Sbjct: 241 IRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVIEWNGNIYDSHELHAESLFFVHPVTGI 300

Query: 339 PLKLQAPLPSWASQALQPQQQEVNSPQSFK 364
           PLKLQAPLPSWASQAL  QQQEV+SP + K
Sbjct: 301 PLKLQAPLPSWASQALLSQQQEVSSPSTLK 330

BLAST of IVF0014656 vs. ExPASy TrEMBL
Match: A0A6J1DT56 (RNA pseudouridine synthase 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111023261 PE=4 SV=1)

HSP 1 Score: 559.7 bits (1441), Expect = 9.1e-156
Identity = 279/340 (82.06%), Postives = 305/340 (89.71%), Query Frame = 0

Query: 39  MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 98
           M D S PVTGN P+TG+NYP+PLSPPLPAISKNLELARAMAASSKSSLFALSA DVI+ED
Sbjct: 1   MADPSIPVTGNSPATGQNYPIPLSPPLPAISKNLELARAMAASSKSSLFALSATDVIFED 60

Query: 99  EWLIAVNKPQGIYCENVLAAVPRLLCDSANAG---------------IKTNLPELHLANR 158
           EWLIAVNKPQG+YCE+VLA+VPRLL DSA AG               I+ +LPELHLANR
Sbjct: 61  EWLIAVNKPQGVYCESVLASVPRLLGDSAMAGEQSGGTTTLCLCLARIQISLPELHLANR 120

Query: 159 LDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFG 218
           LDRDTSGVMVITKSHKVASKLVKAFTDH VSKSYIAFCVG++PKWKKI+VKSGHGRSKFG
Sbjct: 121 LDRDTSGVMVITKSHKVASKLVKAFTDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFG 180

Query: 219 VWRVYAAADVGRSLPGGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLV 278
           VWRVYAAADVGRSLPGGS+VRDMETYFEVLSVNG++TM+EL + RK+EEETIVV +KSL+
Sbjct: 181 VWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGQSTMDELPEVRKDEEETIVVQSKSLI 240

Query: 279 DIDSQKDEVLIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAES 338
           DID+ KDE+LIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGV EWNG  YDSHELHAES
Sbjct: 241 DIDAHKDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVIEWNGNIYDSHELHAES 300

Query: 339 LYFVHPITGIPLKLQAPLPSWASQALQPQQQEVNSPQSFK 364
           L+FVHP+TGIPLKLQAPLPSWASQAL  QQQEV+SP + K
Sbjct: 301 LFFVHPVTGIPLKLQAPLPSWASQALLSQQQEVSSPSTLK 340

BLAST of IVF0014656 vs. NCBI nr
Match: XP_004144814.3 (RNA pseudouridine synthase 1 [Cucumis sativus] >KGN60992.1 hypothetical protein Csa_021313 [Cucumis sativus])

HSP 1 Score: 696 bits (1796), Expect = 4.07e-252
Identity = 347/366 (94.81%), Postives = 355/366 (96.99%), Query Frame = 0

Query: 1   MALFISLHPNFSIFPKTFAKFPFTKPFCFHRFKSVSNSMTDSSEPVTGNCPSTGENYPVP 60
           MALFISLHPNFSI PKTF +FPFTK FCFHRFKSVSNSMTDSSEPVTGNCPST ENYPVP
Sbjct: 1   MALFISLHPNFSISPKTFTEFPFTKSFCFHRFKSVSNSMTDSSEPVTGNCPSTAENYPVP 60

Query: 61  LSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCENVLAAVP 120
           LSPPLPAISKNLELARAM ASSKSSL+ALSANDVIYEDEWLIAVNKPQGIYCENVLAAVP
Sbjct: 61  LSPPLPAISKNLELARAMVASSKSSLYALSANDVIYEDEWLIAVNKPQGIYCENVLAAVP 120

Query: 121 RLLCDSANAGIKTNLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAF 180
           RLL DSANAGIKT+LPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAF
Sbjct: 121 RLLGDSANAGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAF 180

Query: 181 CVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDMETYFEVLSVNGKNT 240
           CVGTSPKWKKI+VKSGHGRSKFGVWRVYAAADVGRSLPGGS+VRDMETYFEVLSVNGKNT
Sbjct: 181 CVGTSPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGKNT 240

Query: 241 MEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARPRSGRTHQIRLHCQYLGIPIRGD 300
           MEELQKFR+ EEETIVVHTKSLVDIDS KDE+LIRARPRSGRTHQIRLHCQYLGIPIRGD
Sbjct: 241 MEELQKFRRGEEETIVVHTKSLVDIDSHKDEILIRARPRSGRTHQIRLHCQYLGIPIRGD 300

Query: 301 VKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQAPLPSWASQALQPQQQEVNSPQ 360
           VKYEGVTEWN K YDSHELHAESLYFVHP+TGIPLKLQAPLPSWASQALQPQQ EVNSPQ
Sbjct: 301 VKYEGVTEWNEKIYDSHELHAESLYFVHPVTGIPLKLQAPLPSWASQALQPQQHEVNSPQ 360

Query: 361 SFKTMP 366
           SFKTMP
Sbjct: 361 SFKTMP 366

BLAST of IVF0014656 vs. NCBI nr
Match: XP_008453651.1 (PREDICTED: RNA pseudouridine synthase 1 [Cucumis melo] >KAA0048062.1 RNA pseudouridine synthase 1 [Cucumis melo var. makuwa] >TYJ96474.1 RNA pseudouridine synthase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 656 bits (1692), Expect = 6.94e-237
Identity = 328/328 (100.00%), Postives = 328/328 (100.00%), Query Frame = 0

Query: 39  MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 98
           MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED
Sbjct: 1   MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 60

Query: 99  EWLIAVNKPQGIYCENVLAAVPRLLCDSANAGIKTNLPELHLANRLDRDTSGVMVITKSH 158
           EWLIAVNKPQGIYCENVLAAVPRLLCDSANAGIKTNLPELHLANRLDRDTSGVMVITKSH
Sbjct: 61  EWLIAVNKPQGIYCENVLAAVPRLLCDSANAGIKTNLPELHLANRLDRDTSGVMVITKSH 120

Query: 159 KVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLP 218
           KVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLP
Sbjct: 121 KVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLP 180

Query: 219 GGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARP 278
           GGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARP
Sbjct: 181 GGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARP 240

Query: 279 RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQ 338
           RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQ
Sbjct: 241 RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQ 300

Query: 339 APLPSWASQALQPQQQEVNSPQSFKTMP 366
           APLPSWASQALQPQQQEVNSPQSFKTMP
Sbjct: 301 APLPSWASQALQPQQQEVNSPQSFKTMP 328

BLAST of IVF0014656 vs. NCBI nr
Match: XP_038889468.1 (RNA pseudouridine synthase 1 [Benincasa hispida])

HSP 1 Score: 632 bits (1630), Expect = 5.53e-227
Identity = 320/359 (89.14%), Postives = 334/359 (93.04%), Query Frame = 0

Query: 1   MALFISLHPNFSIFPKTFAKFPFTKPFCFHRFKSVSNSMTDSSEPVTGNCPSTGENYPVP 60
           MAL ISL P FS   KTF +FP  KPFC  RFKS+S SM DSSEPVTGNCPST ENYPVP
Sbjct: 1   MALLISLQPTFSTPHKTFTEFP--KPFCSGRFKSISKSMADSSEPVTGNCPSTRENYPVP 60

Query: 61  LSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCENVLAAVP 120
           LSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCE+VLAAVP
Sbjct: 61  LSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCESVLAAVP 120

Query: 121 RLLCDSANAGIKTNLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAF 180
           RLL DSA AGIKT+LPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAF
Sbjct: 121 RLLSDSAMAGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAF 180

Query: 181 CVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDMETYFEVLSVNGKNT 240
           CVG++PKWKKI++KSGHGRSKFGVWRVYAAADVGRSLPGGS+VRDMETYFEVLS+NG+N 
Sbjct: 181 CVGSAPKWKKINIKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSINGQNA 240

Query: 241 MEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARPRSGRTHQIRLHCQYLGIPIRGD 300
           MEE QKF K+EE T+VV TKSLVDID QKDE+LIRARPRSGRTHQIRLHCQYLGIPIRGD
Sbjct: 241 MEEFQKFGKDEE-TVVVQTKSLVDIDGQKDEILIRARPRSGRTHQIRLHCQYLGIPIRGD 300

Query: 301 VKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQAPLPSWASQALQPQQQEVNSP 359
           VKYEGVTEWNGK YD HELHAESLYFVHPITGIPLKLQAPLPSWASQA QPQQQEVNSP
Sbjct: 301 VKYEGVTEWNGKIYDCHELHAESLYFVHPITGIPLKLQAPLPSWASQAWQPQQQEVNSP 356

BLAST of IVF0014656 vs. NCBI nr
Match: KAG7021056.1 (RNA pseudouridine synthase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 583 bits (1502), Expect = 3.12e-207
Identity = 305/375 (81.33%), Postives = 329/375 (87.73%), Query Frame = 0

Query: 1   MALFISLHPNFSIFPKTFAKFPFTKPFCFHRFKSVSNSMTDSSEPVTGNCPSTGENYPVP 60
           MAL +++ P FSI  K+FAKFP  KPF FHRF+ V+NSM DSS+PVTG      ENYPVP
Sbjct: 6   MALVVTIQPPFSIPHKSFAKFP--KPFRFHRFRPVTNSMADSSKPVTG------ENYPVP 65

Query: 61  LSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCENVLAAVP 120
           LSPPLPAISKNLELARAMAA SKSSLF+LSA DVIYEDEWLIAVNKPQG+YCE+VLA+VP
Sbjct: 66  LSPPLPAISKNLELARAMAACSKSSLFSLSACDVIYEDEWLIAVNKPQGVYCESVLASVP 125

Query: 121 RLLCDSANAG-----IKTNLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSK 180
           R L DSA AG     IK +LPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDH VSK
Sbjct: 126 RFLGDSAMAGAQSGEIKISLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHKVSK 185

Query: 181 SYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDMETYFEVLSV 240
           SYIAFCVG++PKWKKI VKSGHGRSKFGVWRVYAAADVGRSLPGGS+VRDMET FEVLSV
Sbjct: 186 SYIAFCVGSAPKWKKIIVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETSFEVLSV 245

Query: 241 NGKNTMEELQKFRK----EEEETIVVHTKSLVDIDSQKDEVLIRARPRSGRTHQIRLHCQ 300
           NG+NT EEL + RK    EEEETIV  +KSL+DID++KDE+LIRARPRSGRTHQIRLH Q
Sbjct: 246 NGQNTKEELPESRKDEEEEEEETIVAQSKSLIDIDARKDEILIRARPRSGRTHQIRLHSQ 305

Query: 301 YLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQAPLPSWASQALQP 360
           YLGIPI GDVKYEGVTEWNGK YDSHELHAESLYFVHP+TGIPLKLQAPLPSWA+QALQP
Sbjct: 306 YLGIPIVGDVKYEGVTEWNGKIYDSHELHAESLYFVHPVTGIPLKLQAPLPSWATQALQP 365

Query: 361 QQQEVNSPQSFKTMP 366
           QQQEVNS  S K MP
Sbjct: 366 QQQEVNSAASLKPMP 372

BLAST of IVF0014656 vs. NCBI nr
Match: KAG6586224.1 (RNA pseudouridine synthase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 576 bits (1485), Expect = 1.08e-204
Identity = 303/377 (80.37%), Postives = 327/377 (86.74%), Query Frame = 0

Query: 1   MALFISLHPNFSIFPKTFAKFPFTKPFCFHRFKSVSNSMTDSSEPVTGNCPSTGENYPVP 60
           MAL ++L   FSI  K+FAKFP  KPF FHRF+ V+NSM DSS+PVTG      ENYPVP
Sbjct: 1   MALVVTLQAPFSIPNKSFAKFP--KPFRFHRFRPVTNSMADSSKPVTG------ENYPVP 60

Query: 61  LSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCENVLAAVP 120
           LSPPLPAISKNLELARAMAA SKSSLF+LSA DV+YEDEWLIAVNKPQG+YCE+VLA+VP
Sbjct: 61  LSPPLPAISKNLELARAMAACSKSSLFSLSACDVVYEDEWLIAVNKPQGVYCESVLASVP 120

Query: 121 RLLCDSANAG-----IKTNLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSK 180
             L DSA AG     IK +LPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDH VSK
Sbjct: 121 CFLGDSAMAGEQSGEIKISLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHKVSK 180

Query: 181 SYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDMETYFEVLSV 240
           SYIAFCVG++PKWKKI VKSGHGRSKFGVWRVYAAADVGRSLPGGS+VRDMET FEVLSV
Sbjct: 181 SYIAFCVGSAPKWKKIIVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETSFEVLSV 240

Query: 241 NGKNTMEELQKFRK------EEEETIVVHTKSLVDIDSQKDEVLIRARPRSGRTHQIRLH 300
           NG+NT EEL + RK      EEEETIV  +KSL+DID++KDE+LIRARPRSGRTHQIRLH
Sbjct: 241 NGQNTKEELPESRKDEEEEEEEEETIVAQSKSLIDIDARKDEILIRARPRSGRTHQIRLH 300

Query: 301 CQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQAPLPSWASQAL 360
            QYLGIPI GDVKYEGVTEWNGK YDSHELHAESLYFVHP+TGIPLKLQAPLPSWA+QAL
Sbjct: 301 SQYLGIPIVGDVKYEGVTEWNGKIYDSHELHAESLYFVHPVTGIPLKLQAPLPSWATQAL 360

Query: 361 QPQQQEVNSPQSFKTMP 366
           QPQQQEVNS  S K MP
Sbjct: 361 QPQQQEVNSAASLKPMP 369

BLAST of IVF0014656 vs. TAIR 10
Match: AT1G56345.1 (Pseudouridine synthase family protein )

HSP 1 Score: 361.3 bits (926), Expect = 9.1e-100
Identity = 181/294 (61.56%), Postives = 223/294 (75.85%), Query Frame = 0

Query: 56  NYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCENV 115
           NYP P+S P P ISK++EL RAM ASSKSSLF L+ +D++YEDE+L+AVNKP+G+YCE V
Sbjct: 39  NYPKPISAPPPPISKDIELRRAMEASSKSSLFNLTRDDILYEDEYLMAVNKPKGVYCEAV 98

Query: 116 LAAVPRLLCDSANAGIKTNLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSK 175
           L + P+++ DS++        E HLANRLDRDTSGVM+ITKSHKVA+KLVKAFT+H + K
Sbjct: 99  LRSAPQIVLDSSS--------EYHLANRLDRDTSGVMIITKSHKVAAKLVKAFTEHKIRK 158

Query: 176 SYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDMETYFEVLSV 235
           SYIA C+G+SP W+++ V SGHGRSK G WRVYAA DVGR LPGGS VRDMET FEV+SV
Sbjct: 159 SYIALCIGSSPNWRRVTVSSGHGRSKHGAWRVYAALDVGRVLPGGSFVRDMETTFEVVSV 218

Query: 236 NG-KNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDE--VLIRARPRSGRTHQIRLHCQY 295
           N  KN   EL+    +    IV   +  +      D+  V++RA PRSGRTHQIRLHCQY
Sbjct: 219 NSVKNESCELE----DVNHVIVAEGERELSCGGDDDDVVVVVRAFPRSGRTHQIRLHCQY 278

Query: 296 LGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQAPLPSWAS 347
           LGIPIRGDVKY GV EWNG+T++ HELHAE L   HP+TG  + ++APLP WA+
Sbjct: 279 LGIPIRGDVKYHGVYEWNGRTFEGHELHAECLSLDHPVTGDSIVIRAPLPYWAA 320

BLAST of IVF0014656 vs. TAIR 10
Match: AT1G76050.2 (Pseudouridine synthase family protein )

HSP 1 Score: 72.0 bits (175), Expect = 1.1e-12
Identity = 73/304 (24.01%), Postives = 114/304 (37.50%), Query Frame = 0

Query: 93  DVIYEDEWLIAVNK------------PQGIYCENVLAAVPRLLCDSANA----------- 152
           D++YED+ ++ VNK            P G     +L        D +N+           
Sbjct: 149 DIVYEDKHVLVVNKPAHMVVHPAPGNPTGTLVNGILHHCSLPCVDYSNSEEDDDSDEETF 208

Query: 153 ---GIKTNLPELHLA-------NRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIA 212
                 T  P  + A       +RLD+ T+G++V+ K     + L + F  HT+ + Y++
Sbjct: 209 SDDEEMTTSPSSYAASVRPGIVHRLDKGTTGLLVVAKDEHSHAHLAEQFKLHTIERVYVS 268

Query: 213 FCVGT-SPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDMETYFEVLSVNGK 272
              G  SP   +I +  G   S     R+  AA     +PGG              V G 
Sbjct: 269 LTTGVPSPPQGRIEIPIGRDSSN----RIRMAA-----IPGG--------------VRGG 328

Query: 273 NTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARPRSGRTHQIRLHCQYLGIPIR 332
                  +++             +++  +     L+  R  +GRTHQIR H +Y+G+P+ 
Sbjct: 329 RARHAASRYK-------------VIETFAGGGSALVEWRLETGRTHQIRAHAKYMGVPLL 388

Query: 333 GDVKYEG-------VTEWNGKTYDSHE------------LHAESLYFVHPITGIPLKLQA 344
           GD  Y G       + +      D  E            LHA  L F HP TG  +K   
Sbjct: 389 GDEVYGGTKSMALSLLQKRVSRSDQEEIIELISRMDRPCLHAIVLGFTHPCTGEIVKFSC 416

BLAST of IVF0014656 vs. TAIR 10
Match: AT3G19440.1 (Pseudouridine synthase family protein )

HSP 1 Score: 61.2 bits (147), Expect = 2.0e-09
Identity = 76/279 (27.24%), Postives = 110/279 (39.43%), Query Frame = 0

Query: 52  STGENYPVPLS-----PPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNK 111
           + G+   +PLS     P  P   K      +   S +   F  S   V+Y+D  +I +NK
Sbjct: 145 NVGDRIYLPLSVDNDTPQTPPAKK-----ESFQCSDEERKFVCSL--VLYKDPAIIVLNK 204

Query: 112 PQGIYCENVLAAVPRLLCDSANAGIKTNLPEL-------------HLANRLDRDTSGVMV 171
           P G             L     +GIKT++ EL              L +RLDRD SG++V
Sbjct: 205 PHG-------------LAVQGGSGIKTSIDELAASCLKFDKSESPRLVHRLDRDCSGLLV 264

Query: 172 ITKSHKVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADV 231
           + ++   A+ L   F + T          G S    K +VKS        + R Y A  +
Sbjct: 265 LARTQTAATVLHSIFREKT---------TGASAYGVKKNVKS--------LKRKYMALVI 324

Query: 232 G--------RSLPGGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDI 291
           G         S P   +V D +   E ++VN    +   Q             T+  V  
Sbjct: 325 GCPPRQRGQISAPLRKVVVD-DGKSERITVNDNGELVSTQH----------AITEYRVIE 375

Query: 292 DSQKDEVLIRARPRSGRTHQIRLHC-QYLGIPIRGDVKY 304
            S      +  RP +GR HQ+R+HC + LG PI GD KY
Sbjct: 385 SSPHGYTWLELRPLTGRKHQLRVHCAEVLGTPIVGDYKY 375

BLAST of IVF0014656 vs. TAIR 10
Match: AT3G52260.2 (Pseudouridine synthase family protein )

HSP 1 Score: 55.8 bits (133), Expect = 8.2e-08
Identity = 58/223 (26.01%), Postives = 92/223 (41.26%), Query Frame = 0

Query: 93  DVIYEDEWLIAVNKPQGIYC--------ENVLAAVPRLLCDSANAGIKTNLPELHLANRL 152
           +V+YED+ LIA+NKP G+            VL  +      + +       P     +RL
Sbjct: 98  EVLYEDDDLIALNKPSGLQVLPGGLFQQRTVLTQLQWCFGKNDSYIGSRESPHPVPVHRL 157

Query: 153 DRDTSGVMVITKSHKVASKLVKAFTDHT--VSKSYIAFCVGTSPKWKKIHVKSGHGRSKF 212
            R TSG+++  K+    +KL   F + T  V    +    GT  K  KI+     G    
Sbjct: 158 GRGTSGILLCAKTKLAKTKLAAYFAEGTSLVGSGNLDQECGTGRKLSKIYRALADG---- 217

Query: 213 GVWRVYAAADVGRSLPGGSIVR--DMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTK 272
               +    +V    P G +VR   +     V S  GK    ++                
Sbjct: 218 ----IVEEDEVVIKQPIG-VVRYPGVAQGLYVASPEGKPAFSKV---------------- 277

Query: 273 SLVDIDSQKDEVLIRARPRSGRTHQIRLHCQYLGIPIRGDVKY 304
            +++ D +K+  L++   +SGR HQIR+H  Y+G P+ GD  Y
Sbjct: 278 FVLERDREKNCSLVKVEIQSGRPHQIRIHLAYMGHPLVGDPLY 295

BLAST of IVF0014656 vs. TAIR 10
Match: AT5G51140.1 (Pseudouridine synthase family protein )

HSP 1 Score: 52.4 bits (124), Expect = 9.1e-07
Identity = 52/196 (26.53%), Postives = 75/196 (38.27%), Query Frame = 0

Query: 134 NLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHV 193
           +L  L   +RLDR  SG+++I ++   A    +      V K YIA  +G  P+ + I  
Sbjct: 176 DLGTLFPIHRLDRLVSGLLIIARTAAKADFFRQQIEGGMVKKRYIAKVIGVFPEDEMI-- 235

Query: 194 KSGHGRSKFGVWRVYAAADVGRSLPGGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEE 253
                        V A  +   S  G S   D  +  +   V GK    +  +       
Sbjct: 236 -------------VDANINYNGS-EGRSTAEDANSSGDDKKVKGKPACTKFTR------- 295

Query: 254 TIVVHTKSLVDIDSQKDEVLIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKT 313
                      ID+     L+   P +GRTHQIR+H QY G PI  D  Y      N +T
Sbjct: 296 -----------IDTNGTHSLVSCEPVTGRTHQIRVHLQYTGHPIANDPLYLNQHIDNLET 337

Query: 314 YDSHELHAESLYFVHP 330
           Y +  + A     V P
Sbjct: 356 YIAKRIDAGERKIVSP 337

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q2QNM31.2e-10161.00RNA pseudouridine synthase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g05... [more]
Q7XA651.3e-9861.56RNA pseudouridine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g56345 PE=2 S... [more]
Q68XB22.9e-1831.25Ribosomal large subunit pseudouridine synthase C OS=Rickettsia typhi (strain ATC... [more]
Q87S654.2e-1727.74Ribosomal large subunit pseudouridine synthase D OS=Vibrio parahaemolyticus sero... [more]
Q9ZDR73.6e-1628.13Ribosomal large subunit pseudouridine synthase C OS=Rickettsia prowazekii (strai... [more]
Match NameE-valueIdentityDescription
A0A0A0LJY65.6e-19894.81PseudoU_synth_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G03... [more]
A0A5A7TX255.0e-186100.00RNA pseudouridine synthase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3BW875.0e-186100.00RNA pseudouridine synthase 1 OS=Cucumis melo OX=3656 GN=LOC103494301 PE=4 SV=1[more]
A0A6J1DQ126.3e-15784.55RNA pseudouridine synthase 1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A6J1DT569.1e-15682.06RNA pseudouridine synthase 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111... [more]
Match NameE-valueIdentityDescription
XP_004144814.34.07e-25294.81RNA pseudouridine synthase 1 [Cucumis sativus] >KGN60992.1 hypothetical protein ... [more]
XP_008453651.16.94e-237100.00PREDICTED: RNA pseudouridine synthase 1 [Cucumis melo] >KAA0048062.1 RNA pseudou... [more]
XP_038889468.15.53e-22789.14RNA pseudouridine synthase 1 [Benincasa hispida][more]
KAG7021056.13.12e-20781.33RNA pseudouridine synthase 1, partial [Cucurbita argyrosperma subsp. argyrosperm... [more]
KAG6586224.11.08e-20480.37RNA pseudouridine synthase 1, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT1G56345.19.1e-10061.56Pseudouridine synthase family protein [more]
AT1G76050.21.1e-1224.01Pseudouridine synthase family protein [more]
AT3G19440.12.0e-0927.24Pseudouridine synthase family protein [more]
AT3G52260.28.2e-0826.01Pseudouridine synthase family protein [more]
AT5G51140.19.1e-0726.53Pseudouridine synthase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006145Pseudouridine synthase, RsuA/RluA-likePFAMPF00849PseudoU_synth_2coord: 101..291
e-value: 1.1E-24
score: 87.4
IPR006145Pseudouridine synthase, RsuA/RluA-likeCDDcd02869PseudoU_synth_RluA_likecoord: 101..326
e-value: 1.52769E-40
score: 139.395
NoneNo IPR availableGENE3D3.30.2350.10Pseudouridine synthasecoord: 89..342
e-value: 4.6E-46
score: 159.1
NoneNo IPR availablePANTHERPTHR21600MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASEcoord: 45..353
NoneNo IPR availablePANTHERPTHR21600:SF47RNA PSEUDOURIDINE SYNTHASE 1coord: 45..353
IPR006224Pseudouridine synthase, RluC/RluD, conserved sitePROSITEPS01129PSI_RLUcoord: 141..155
IPR020103Pseudouridine synthase, catalytic domain superfamilySUPERFAMILY55120Pseudouridine synthasecoord: 92..351

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0014656.1IVF0014656.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0001522 pseudouridine synthesis
biological_process GO:0009451 RNA modification
molecular_function GO:0009982 pseudouridine synthase activity
molecular_function GO:0003723 RNA binding