IVF0010812 (gene) Melon (IVF77) v1

Overview
NameIVF0010812
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Descriptioncytochrome P450 CYP736A12-like
Locationchr08: 5213032 .. 5214811 (-)
RNA-Seq ExpressionIVF0010812
SyntenyIVF0010812
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACTTGGATTATTTTGGCCACAATTACACTCATCTTCCTCCTCCCTTACCTCTTTCAACAATGGCTTCTCAAAACCAAACCCTTATGCAACAAACTCCCTCCTGGTCCAAAAGGGTTTCCAATTATTGGAAGCCTTCATCTATTAGGCAAACTCATTCACAAAGATCTTCATTACTTATCCCAAATATACGGTCCCATAATGCACATCCAATTAGGTCTTCTCCCCGCCATCATTGTCTCCTCCGCTAGAGCCACCGAGCTCTTCCTTAAAACTCACGACCTTCACTTTGCGAGCCGCCCTCTTACCATCACCTCCAACCATATTTCTTACGGTAGAAAGGGTATAGTTTTTGCTCAATATGGTCCTTATTGGCGCAATATTCGCAAAATGTGCACTCTTGAATTGCTTAGCTCCTTAAAAATCAACTCTTTTTCCTCAATGAGAAAGCAGGAGGTTGGTTCATTGATTAAGGGTCTTGAAGATGCTGCTACAGATGGTGTGGCTGTGGATCTTACTTCTAAAATTTCTTCTGTTATTGGAGATATGATATGTGTAATGGTGTTGGGGAGAAAATATGAAGATAATGAGTTGGGTGAAAAGGGGTTTAAGGGTTTGATACGAGAGGCTACACAATTAGCGGCAGCTCCTAATTTGGGAGATTTTATTCCTTTAATTGCAAGGTTTGATGTCCAAGGATTTGGGGGTCGCGCAAAAGCTGTTGGGAAAATATTTGACGGGTTTTTAGAGAGTATTGTTGAAGAACATGTTGTGTTCGAGAAGGATAATAAAGACAAGGATTTTGTGGACGTTTTGTTGGATTTGATGGGGTCTCGAGAGTACCAAATTGATCGATCAAATATCAAAGCTATAATTCTGGTACGAATTGATTGACTGTAGAATGTGGAGTGTTTTTGGATTTCCTTTTTCTTTGATGATTAAATTGAAAAGAAAAATGAAAATATATTAAGAAAAAATAGCATTTTTAAAAGAGTTGTTTTCAGTAACATGAATAAAAATATTTATAGATATAGTAAAAAGTTGCGCTTTATTAGTGATAAGCGATCATAAATGTAAATAATTGAAAATTTTGGTAAACTCATTTAAAATTATTTTCAAGAATGTAACTAGAGGTAGGAATATTTCTTAATTTTTGCAGGATTTTGTTATTGCAGCCGTGGATAGTACAACAACAACAATCAATTGGATGTTATCGGAACTTATTAAGCATCCACATATAATGAAAAAGTTGCAAGAAGAATTAGAAAAAGTGGTGGGATTGAATAGAATGGTGGAAGAATCAGACTTAAGTAACTTGAAATACTTAGAGATGGTAATGAAAGAGAGCTTGAGAATGCATCCACCAGTTCCATTAATTCCACGAGAATGCATACAAGATTGCAATATAAATGGGTATCATATCCCTAAGAAATCAAGAATAGTAATAAATGCATGGGCCATTGGAAGAGATCCAAATACTTGGGTTGATCCTCATAAGTTTGATCCAGAGAGATTTTTGGAAAGTGAAGTAGATGTGAGAGGGAGAGATTTTGAGCTAATTCCATTTGGATCAGGTCGAAGAGGCTGTGTTGGAATACAATTAGCTTTGGTTGTGGTTCGTCTTGTAGTGGCTCAATTATTACACTGTTTTGATTTGAAGCTTCCCGATGGTATGTCACCACTTGAATTGGATATGACAGAAATTTTAGGGTTGACTTGTCCTAGAGCCCAAAATCTTAGGGTGATTCCCATTTTTCGTCTTTGTCCTAATTATTTATAA

mRNA sequence

ATGACTTGGATTATTTTGGCCACAATTACACTCATCTTCCTCCTCCCTTACCTCTTTCAACAATGGCTTCTCAAAACCAAACCCTTATGCAACAAACTCCCTCCTGGTCCAAAAGGGTTTCCAATTATTGGAAGCCTTCATCTATTAGGCAAACTCATTCACAAAGATCTTCATTACTTATCCCAAATATACGGTCCCATAATGCACATCCAATTAGGTCTTCTCCCCGCCATCATTGTCTCCTCCGCTAGAGCCACCGAGCTCTTCCTTAAAACTCACGACCTTCACTTTGCGAGCCGCCCTCTTACCATCACCTCCAACCATATTTCTTACGGTAGAAAGGGTATAGTTTTTGCTCAATATGGTCCTTATTGGCGCAATATTCGCAAAATGTGCACTCTTGAATTGCTTAGCTCCTTAAAAATCAACTCTTTTTCCTCAATGAGAAAGCAGGAGGTTGGTTCATTGATTAAGGGTCTTGAAGATGCTGCTACAGATGGTGTGGCTGTGGATCTTACTTCTAAAATTTCTTCTGTTATTGGAGATATGATATGTGTAATGGTGTTGGGGAGAAAATATGAAGATAATGAGTTGGGTGAAAAGGGGTTTAAGGGTTTGATACGAGAGGCTACACAATTAGCGGCAGCTCCTAATTTGGGAGATTTTATTCCTTTAATTGCAAGGTTTGATGTCCAAGGATTTGGGGGTCGCGCAAAAGCTGTTGGGAAAATATTTGACGGGTTTTTAGAGAGTATTGTTGAAGAACATGTTGTGTTCGAGAAGGATAATAAAGACAAGGATTTTGTGGACGTTTTGTTGGATTTGATGGGGTCTCGAGAGTACCAAATTGATCGATCAAATATCAAAGCTATAATTCTGGATTTTGTTATTGCAGCCGTGGATAGTACAACAACAACAATCAATTGGATGTTATCGGAACTTATTAAGCATCCACATATAATGAAAAAGTTGCAAGAAGAATTAGAAAAAGTGGTGGGATTGAATAGAATGGTGGAAGAATCAGACTTAAGTAACTTGAAATACTTAGAGATGGTAATGAAAGAGAGCTTGAGAATGCATCCACCAGTTCCATTAATTCCACGAGAATGCATACAAGATTGCAATATAAATGGGTATCATATCCCTAAGAAATCAAGAATAGTAATAAATGCATGGGCCATTGGAAGAGATCCAAATACTTGGGTTGATCCTCATAAGTTTGATCCAGAGAGATTTTTGGAAAGTGAAGTAGATGTGAGAGGGAGAGATTTTGAGCTAATTCCATTTGGATCAGGTCGAAGAGGCTGTGTTGGAATACAATTAGCTTTGGTTGTGGTTCGTCTTGTAGTGGCTCAATTATTACACTGTTTTGATTTGAAGCTTCCCGATGGTATGTCACCACTTGAATTGGATATGACAGAAATTTTAGGGTTGACTTGTCCTAGAGCCCAAAATCTTAGGGTGATTCCCATTTTTCGTCTTTGTCCTAATTATTTATAA

Coding sequence (CDS)

ATGACTTGGATTATTTTGGCCACAATTACACTCATCTTCCTCCTCCCTTACCTCTTTCAACAATGGCTTCTCAAAACCAAACCCTTATGCAACAAACTCCCTCCTGGTCCAAAAGGGTTTCCAATTATTGGAAGCCTTCATCTATTAGGCAAACTCATTCACAAAGATCTTCATTACTTATCCCAAATATACGGTCCCATAATGCACATCCAATTAGGTCTTCTCCCCGCCATCATTGTCTCCTCCGCTAGAGCCACCGAGCTCTTCCTTAAAACTCACGACCTTCACTTTGCGAGCCGCCCTCTTACCATCACCTCCAACCATATTTCTTACGGTAGAAAGGGTATAGTTTTTGCTCAATATGGTCCTTATTGGCGCAATATTCGCAAAATGTGCACTCTTGAATTGCTTAGCTCCTTAAAAATCAACTCTTTTTCCTCAATGAGAAAGCAGGAGGTTGGTTCATTGATTAAGGGTCTTGAAGATGCTGCTACAGATGGTGTGGCTGTGGATCTTACTTCTAAAATTTCTTCTGTTATTGGAGATATGATATGTGTAATGGTGTTGGGGAGAAAATATGAAGATAATGAGTTGGGTGAAAAGGGGTTTAAGGGTTTGATACGAGAGGCTACACAATTAGCGGCAGCTCCTAATTTGGGAGATTTTATTCCTTTAATTGCAAGGTTTGATGTCCAAGGATTTGGGGGTCGCGCAAAAGCTGTTGGGAAAATATTTGACGGGTTTTTAGAGAGTATTGTTGAAGAACATGTTGTGTTCGAGAAGGATAATAAAGACAAGGATTTTGTGGACGTTTTGTTGGATTTGATGGGGTCTCGAGAGTACCAAATTGATCGATCAAATATCAAAGCTATAATTCTGGATTTTGTTATTGCAGCCGTGGATAGTACAACAACAACAATCAATTGGATGTTATCGGAACTTATTAAGCATCCACATATAATGAAAAAGTTGCAAGAAGAATTAGAAAAAGTGGTGGGATTGAATAGAATGGTGGAAGAATCAGACTTAAGTAACTTGAAATACTTAGAGATGGTAATGAAAGAGAGCTTGAGAATGCATCCACCAGTTCCATTAATTCCACGAGAATGCATACAAGATTGCAATATAAATGGGTATCATATCCCTAAGAAATCAAGAATAGTAATAAATGCATGGGCCATTGGAAGAGATCCAAATACTTGGGTTGATCCTCATAAGTTTGATCCAGAGAGATTTTTGGAAAGTGAAGTAGATGTGAGAGGGAGAGATTTTGAGCTAATTCCATTTGGATCAGGTCGAAGAGGCTGTGTTGGAATACAATTAGCTTTGGTTGTGGTTCGTCTTGTAGTGGCTCAATTATTACACTGTTTTGATTTGAAGCTTCCCGATGGTATGTCACCACTTGAATTGGATATGACAGAAATTTTAGGGTTGACTTGTCCTAGAGCCCAAAATCTTAGGGTGATTCCCATTTTTCGTCTTTGTCCTAATTATTTATAA

Protein sequence

MTWIILATITLIFLLPYLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYLSQIYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFASRPLTITSNHISYGRKGIVFAQYGPYWRNIRKMCTLELLSSLKINSFSSMRKQEVGSLIKGLEDAATDGVAVDLTSKISSVIGDMICVMVLGRKYEDNELGEKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKAVGKIFDGFLESIVEEHVVFEKDNKDKDFVDVLLDLMGSREYQIDRSNIKAIILDFVIAAVDSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVMKESLRMHPPVPLIPRECIQDCNINGYHIPKKSRIVINAWAIGRDPNTWVDPHKFDPERFLESEVDVRGRDFELIPFGSGRRGCVGIQLALVVVRLVVAQLLHCFDLKLPDGMSPLELDMTEILGLTCPRAQNLRVIPIFRLCPNYL
Homology
BLAST of IVF0010812 vs. ExPASy Swiss-Prot
Match: A0A068Q5V6 (Cytochrome P450 71AU50 OS=Prunus mume OX=102107 GN=CYP71AU50 PE=2 SV=1)

HSP 1 Score: 573.9 bits (1478), Expect = 1.7e-162
Identity = 293/498 (58.84%), Postives = 370/498 (74.30%), Query Frame = 0

Query: 1   MTWIILATITLIFLLPYLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYL 60
           M W I ATI L+ L+  L   W  K K    +LPPGP+GFPI GSLHLLG+  +KDLH L
Sbjct: 1   MVW-IWATIGLLALVHILQAWWKNKKK----RLPPGPRGFPIFGSLHLLGEFPNKDLHRL 60

Query: 61  SQIYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFASRPLTITSNHISYGRKGIVFAQ 120
           ++ YG IM+++LGL+P I++SS  A ELFLKTHDL FASRP    S HIS+G+K ++F++
Sbjct: 61  ARKYGDIMYMRLGLMPTIVISSPEAAELFLKTHDLVFASRPPHEGSKHISFGQKNLIFSE 120

Query: 121 YGPYWRNIRKMCTLELLSSLKINSFSSMRKQEVGSLIKGLEDAATD-GVAVDLTSKISSV 180
           YG YWR+ RKMCT+ELLS+ KINSF SMR++EV   ++ +  AA + GVAVDL+ K+SS+
Sbjct: 121 YGAYWRDTRKMCTIELLSNHKINSFKSMRREEVSLCVESIRAAANNRGVAVDLSDKVSSL 180

Query: 181 IGDMICVMVLGRKYEDNELGEKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAK 240
             DM C MVLG+KY D E  E+GFK ++REA QLA+APNLGD+I  IA  D+QGF  R K
Sbjct: 181 SVDMSCRMVLGKKYRDEEFDERGFKSVVREAIQLASAPNLGDYIRFIAPLDLQGFTKRMK 240

Query: 241 AVGKIFDGFLESIVEEHVVFEKDNKDKDFVDVLLDLMGSR--EYQIDRSNIKAIILDFVI 300
           +V K FD   E I+EEH+      +  DFVDV++  MGS   EY+I+R +IKAI+LD ++
Sbjct: 241 SVNKAFDNLFEKIIEEHLQPNDGERTMDFVDVMVGFMGSEESEYRIERPHIKAIMLDMLV 300

Query: 301 AAVDSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVMKESL 360
           A++D++ TTI W LSEL++HP  MKK+Q+ELE VVGL++MVEESDL  L YL MV+KE+ 
Sbjct: 301 ASMDTSATTIEWALSELMRHPKAMKKVQKELENVVGLDKMVEESDLEKLDYLNMVVKETF 360

Query: 361 RMHPPVP-LIPRECIQDCNINGYHIPKKSRIVINAWAIGRDPNTWVDPHKFDPERFLESE 420
           R+HP  P LIP   I+DC +NGYHIPKKSR++IN WAIGRDPN W D  KF PERF  S 
Sbjct: 361 RLHPVAPLLIPHASIEDCTVNGYHIPKKSRVLINVWAIGRDPNAWTDAEKFIPERFEGSS 420

Query: 421 VDVRGRDFELIPFGSGRRGCVGIQLALVVVRLVVAQLLHCFDLKLPDGMSPLELDMTEIL 480
           VDVRG  F+LIPFGSGRR C GIQL L VV+LV+AQL+HCFD +LP+ M P ELDMTE  
Sbjct: 421 VDVRGNHFQLIPFGSGRRRCPGIQLGLTVVQLVLAQLVHCFDWELPNNMLPEELDMTEEF 480

Query: 481 GLTCPRAQNLRVIPIFRL 495
           GLT PRA++L  IP +RL
Sbjct: 481 GLTVPRAKHLLAIPSYRL 493

BLAST of IVF0010812 vs. ExPASy Swiss-Prot
Match: H2DH18 (Cytochrome P450 CYP736A12 OS=Panax ginseng OX=4054 PE=2 SV=1)

HSP 1 Score: 477.2 bits (1227), Expect = 2.2e-133
Identity = 248/497 (49.90%), Postives = 341/497 (68.61%), Query Frame = 0

Query: 11  LIFLLPYLFQQWLLK-TKPL--CNKLPPGPKGFPIIGSLHLLGKLIHKDLHYLSQIYGPI 70
           L+F+L      W+L    PL   +KLPPGP+G PIIGSLH LG L H+ L  L++ YGPI
Sbjct: 8   LLFVLLGALSWWILPIISPLKRHHKLPPGPRGLPIIGSLHTLGALPHRTLQTLAKKYGPI 67

Query: 71  MHIQLGLLPAIIVSSARATELFLKTHDLHFASRPLTITSNHISYGRKGIVFAQYGPYWRN 130
           M ++LG +P I+VSS +A ELFLKTHD  FASRP    + ++SYG  G+ F  YGP+WRN
Sbjct: 68  MSMRLGSVPTIVVSSPQAAELFLKTHDNIFASRPKLQAAEYMSYGTMGMSFTAYGPHWRN 127

Query: 131 IRKMCTLELLSSLKINSFSSMRKQEVGSLIKGLEDAATDGVAVDLTSKISSVIGDMICVM 190
           IRK   LELL+  KINSF  MR++E+G+++K +++A+     VDL++K++++I +M   +
Sbjct: 128 IRKFVVLELLTPAKINSFVGMRREELGTVVKSIKEASAANEVVDLSAKVANIIENMTYRL 187

Query: 191 VLGRKYEDNELGEKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKAVGKIFDG 250
           +LGR  +D        KG++ EA  LA   N+ DF+P +   D+QG   + K  GK  D 
Sbjct: 188 LLGRTKDD----RYDLKGIMNEALTLAGRFNIADFVPFLGPLDIQGLTRQFKDTGKRLDK 247

Query: 251 FLESIVEEHVVFEKD-NKDKDFVDVLLDLMGSREYQ-------IDRSNIKAIILDFVIAA 310
            LE I++EH     + N   DF+D +L L              IDRS IKAI++D + AA
Sbjct: 248 ILEFIIDEHEQNSSNGNASGDFIDDMLSLKNKPSNTHDELSKVIDRSVIKAIMIDIISAA 307

Query: 311 VDSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVMKESLRM 370
           +D++ T+I W+L+ELIKHP  MKK QEE++ VVG++RMVEE+DL NL+Y+ MV+KE LR+
Sbjct: 308 IDTSDTSIEWILTELIKHPRAMKKCQEEIDAVVGVDRMVEETDLPNLEYVYMVVKEGLRL 367

Query: 371 HPPVPLI-PRECIQDCNINGYHIPKKSRIVINAWAIGRDPNTWVD-PHKFDPERFLESEV 430
           HP  PL+ P E ++D  INGY IPK+SR+++N+WA+GRDPN W +   +F PERF  S V
Sbjct: 368 HPVAPLLGPHESMEDITINGYFIPKQSRVIVNSWALGRDPNVWSENAEEFLPERFEGSNV 427

Query: 431 DVRGRDFELIPFGSGRRGCVGIQLALVVVRLVVAQLLHCFDLKLPDGMSPLELDMTEILG 490
           DVRGRDF+L+PFGSGRRGC G+QL L+ V+LVVA+L+HCFD  LP+G +P  LDMTE  G
Sbjct: 428 DVRGRDFQLLPFGSGRRGCPGMQLGLITVQLVVARLVHCFDWNLPNGTTPDNLDMTEKFG 487

Query: 491 LTCPRAQNLRVIPIFRL 495
           LT PR ++L  +P +RL
Sbjct: 488 LTTPRVKHLLAVPKYRL 500

BLAST of IVF0010812 vs. ExPASy Swiss-Prot
Match: P24465 (Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2)

HSP 1 Score: 383.6 bits (984), Expect = 3.3e-105
Identity = 205/485 (42.27%), Postives = 299/485 (61.65%), Query Frame = 0

Query: 4   IILATITLIFLLPYLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYLSQI 63
           ++   I L F L  L ++   + KP    LPP P   PIIG+LH LG L H+ L  L+  
Sbjct: 7   LLFLAIALTFFLLKLNEK--REKKP---NLPPSPPNLPIIGNLHQLGNLPHRSLRSLANE 66

Query: 64  YGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFASRPLTITSNHISYGRKGIVFAQYGP 123
            GP++ + LG +P +IVS+A   E  LKTHDL FASRP T  +  I Y    + F+ YG 
Sbjct: 67  LGPLILLHLGHIPTLIVSTAEIAEEILKTHDLIFASRPSTTAARRIFYDCTDVAFSPYGE 126

Query: 124 YWRNIRKMCTLELLSSLKINSFSSMRKQEVGSLIKGLEDAATDGVAVDLTSKISSVIGDM 183
           YWR +RK+C LELLS  ++NS+ S+R++EVG +++ +  + + G AV+L+  +  +    
Sbjct: 127 YWRQVRKICVLELLSIKRVNSYRSIREEEVGLMMERISQSCSTGEAVNLSELLLLLSSGT 186

Query: 184 ICVMVLGRKYEDNELGEKGFKGLIREATQLAAAPNLGDFIPLIARFDV-QGFGGRAKAVG 243
           I  +  G+KYE  E  +  F  L  E T L  A  +GD+ P  A  DV  G   R K   
Sbjct: 187 ITRVAFGKKYEGEEERKNKFADLATELTTLMGAFFVGDYFPSFAWVDVLTGMDARLKRNH 246

Query: 244 KIFDGFLESIVEEHVVFEKDN-----KDKDFVDVLLDLM--GSREYQIDRSNIKAIILDF 303
              D F++ ++++H++  K N     + KD VDVLL L    S    ++R+N+KA+ILD 
Sbjct: 247 GELDAFVDHVIDDHLLSRKANGSDGVEQKDLVDVLLHLQKDSSLGVHLNRNNLKAVILDM 306

Query: 304 VIAAVDSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVMKE 363
                D+T  T+ W ++ELIKHP +M+K Q+E+ +VVG    VEE DL  L YL++++KE
Sbjct: 307 FSGGTDTTAVTLEWAMAELIKHPDVMEKAQQEVRRVVGKKAKVEEEDLHQLHYLKLIIKE 366

Query: 364 SLRMHPPVP-LIPRECIQDCNINGYHIPKKSRIVINAWAIGRDPNTWVDPHKFDPERFLE 423
           +LR+HP  P L+PRE  +D  I GYHIP K+R+ INAWAIGRDP +W +  +F PERF+ 
Sbjct: 367 TLRLHPVAPLLVPRESTRDVVIRGYHIPAKTRVFINAWAIGRDPKSWENAEEFLPERFVN 426

Query: 424 SEVDVRGRDFELIPFGSGRRGCVGIQLALVVVRLVVAQLLHCFDLKLPDGMSPLELDMTE 480
           + VD +G+DF+LIPFG+GRRGC GI   +  V + +A LL+ F+ +LP  ++  +LDM+E
Sbjct: 427 NSVDFKGQDFQLIPFGAGRRGCPGIAFGISSVEISLANLLYWFNWELPGDLTKEDLDMSE 486

BLAST of IVF0010812 vs. ExPASy Swiss-Prot
Match: A0A068Q721 (Cytochrome P450 71AP13 OS=Prunus mume OX=102107 GN=CYP71AP13 PE=2 SV=1)

HSP 1 Score: 381.7 bits (979), Expect = 1.3e-104
Identity = 200/491 (40.73%), Postives = 304/491 (61.91%), Query Frame = 0

Query: 5   ILATITLIFLLPYLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYLSQIY 64
           + A   LI LL ++++    K++    KLPP P   P+IG+LH LG   H  L  L++ Y
Sbjct: 23  LFAFSLLILLLKFIYKD---KSRKRRVKLPPSPPKLPVIGNLHQLGNKPHLSLRCLAEKY 82

Query: 65  GPIMHIQLGLLPAIIVSSARATELFLKTHDLHFASRPLTITSNHISYGRKGIVFAQYGPY 124
           GPI+++QLG +P ++VSSAR  +  LKTHDL  +SRP   ++ H+ Y    +VF+ YG Y
Sbjct: 83  GPIIYLQLGEIPTVVVSSARLAKEVLKTHDLALSSRPQIFSAKHLFYNCTDVVFSPYGAY 142

Query: 125 WRNIRKMCTLELLSSLKINSFSSMRKQEVGSLIKGLEDAATDGVAVDLTSKISSVIGDMI 184
           WR+IRK+C LELLS+ ++ SFS +R++EV  L++ + +        +LT  +     D++
Sbjct: 143 WRHIRKICILELLSAKRVQSFSHVREEEVARLVRRVAEFYPG--TTNLTKMLGLYANDVL 202

Query: 185 CVMVLGRKY-EDNELGEKGFKGLIREATQLAAAPNLGDFIPLIARF-DVQGFGGRAKAVG 244
           C +  GR + E  +    GF+ ++ E  +L    ++GDF P +     + G   R +   
Sbjct: 203 CRVAFGRGFSEGGDYDRHGFQKMLEEYQELLGGFSIGDFFPSMEFIHSLTGMKSRLQETF 262

Query: 245 KIFDGFLESIVEEHVVFEKDNKD-KDFVDVLLDLM--GSREYQIDRSNIKAIILDFVIAA 304
           + FD   + +V +H+  +++ ++ KD VDVLLD+    S E  +   N+KAIILD   A 
Sbjct: 263 RRFDELFDQMVTDHLSPKREKEEHKDLVDVLLDIQKKESTEMPLTMDNVKAIILDMFAAG 322

Query: 305 VDSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVMKESLRM 364
            D+T  T++W ++EL+ +  ++++ Q E+  VVG  R+V ESDL  L Y++ V+KE  R+
Sbjct: 323 TDTTFITLDWGMTELLMNRKVLERAQAEVRGVVGERRVVLESDLPQLDYMKAVIKEIFRL 382

Query: 365 HPPVP-LIPRECIQDCNINGYHIPKKSRIVINAWAIGRDPNTWVDPHKFDPERFLESEVD 424
           HPP P L+PRE ++D  I+GY I  K+RI +NAWAIGRDP +W DP  F+PERF+ S +D
Sbjct: 383 HPPAPVLVPRESMEDVTIDGYDILAKTRIFVNAWAIGRDPESWEDPEAFEPERFIGSTID 442

Query: 425 VRGRDFELIPFGSGRRGCVGIQLALVVVRLVVAQLLHCFDLKLPDGMSPLELDMTEILGL 484
            +G+DFELIPFG+GRRGC  +      + L +AQLLH FD +LP   +  +LDMTE+ G+
Sbjct: 443 FKGQDFELIPFGAGRRGCPAVTFGTATIELALAQLLHTFDWELPLDTAAKDLDMTEVFGI 502

Query: 485 TCPRAQNLRVI 490
           T  R  NL V+
Sbjct: 503 TMHRIANLIVV 508

BLAST of IVF0010812 vs. ExPASy Swiss-Prot
Match: A0A068Q6L2 (Cytochrome P450 736A117 OS=Prunus mume OX=102107 GN=CYP736A117 PE=2 SV=1)

HSP 1 Score: 377.5 bits (968), Expect = 2.4e-103
Identity = 198/493 (40.16%), Postives = 296/493 (60.04%), Query Frame = 0

Query: 10  TLIFLLPYLFQQWLLKTKPLCNK--LPPGPKGFPIIGSLHLLGKLIHKDLHYLSQIYGPI 69
           TL+ +   L   W   TK    K   PP P   PIIG+LH +G   H+ L  LSQ +GP+
Sbjct: 24  TLLAIFLVLLYTWYSSTKTTTQKSTQPPSPPKLPIIGNLHQIGSYPHRSLQALSQRHGPL 83

Query: 70  MHIQLGLLPAIIVSSARATELFLKTHDLHFASRPLTITSNHISYGRKGIVFAQYGPYWRN 129
           M +  G +P ++VSSA A    LKTHDL F+ RP +     + Y  K +  A YG YWR 
Sbjct: 84  MLLHFGSVPVLVVSSAEAAREILKTHDLTFSDRPKSTIFEKLLYNYKDVASAPYGEYWRQ 143

Query: 130 IRKMCTLELLSSLKINSFSSMRKQEVGSLIKGLEDAATDGVAVDLTSKISSVIGDMICVM 189
           +R +C L LLS+ ++ SF S+R++E  S+I+ ++ +++    ++L+     +  D++C +
Sbjct: 144 VRSICVLNLLSNRRVRSFRSVREEETKSMIRNIKGSSSS--VLNLSEMFVRLTNDVVCKV 203

Query: 190 VLGRKYEDNELGEKG--FKGLIREATQLAAAPNLGDFIPLIARFD-VQGFGGRAKAVGKI 249
            LGRKY D E GE G  FK ++ E   L    N+GD++P ++    V G G +   V K 
Sbjct: 204 ALGRKYSDGEGGESGRMFKEILGEFGDLLGTVNIGDYVPWLSWLSHVNGLGAKLDKVAKQ 263

Query: 250 FDGFLESIVEEHVVFEK---DNKDKDFVDVLLDLM--GSREYQIDRSNIKAIILDFVIAA 309
            D F++++V+EH+       D+  KDF+D+LL +    S    ID  ++K IILD   A 
Sbjct: 264 LDDFIDTVVQEHMNHSSRSGDDDQKDFLDILLAIQKETSAGIPIDGVSVKGIILDMFAAG 323

Query: 310 VDSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNR--MVEESDLSNLKYLEMVMKESL 369
            D+T + + W ++EL++HP +M KLQ E+  +VG NR  ++ E DL  + YL+ V KE+L
Sbjct: 324 TDTTYSALEWAMTELLRHPRVMNKLQNEVRGIVG-NRTDVITEDDLVEMHYLKAVTKETL 383

Query: 370 RMHPPVP-LIPRECIQDCNINGYHIPKKSRIVINAWAIGRDPNTWVDPHKFDPERFLESE 429
           R+HPP+P L+PR   +D  +NGY+I   +++ I+AW IGRDP  +  P +F+PERFL + 
Sbjct: 384 RLHPPIPLLVPRMSTRDVEVNGYNIKANTQVFISAWQIGRDPKLYDKPEEFEPERFLNNG 443

Query: 430 VDVRGRDFELIPFGSGRRGCVGIQLALVVVRLVVAQLLHCFDLKLPDGMSPLELDMTEIL 489
           +D +G DFELIPFG+GRR C GIQ A+ V  + +A ++H FD  LPD  S  +LDMTE  
Sbjct: 444 IDYKGNDFELIPFGAGRRVCPGIQFAMAVNEIALANIVHKFDWALPDEASGEDLDMTETT 503

BLAST of IVF0010812 vs. ExPASy TrEMBL
Match: A0A5D3CM91 (Cytochrome P450 CYP736A12-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002430 PE=3 SV=1)

HSP 1 Score: 998.4 bits (2580), Expect = 1.0e-287
Identity = 499/499 (100.00%), Postives = 499/499 (100.00%), Query Frame = 0

Query: 1   MTWIILATITLIFLLPYLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYL 60
           MTWIILATITLIFLLPYLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYL
Sbjct: 1   MTWIILATITLIFLLPYLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYL 60

Query: 61  SQIYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFASRPLTITSNHISYGRKGIVFAQ 120
           SQIYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFASRPLTITSNHISYGRKGIVFAQ
Sbjct: 61  SQIYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFASRPLTITSNHISYGRKGIVFAQ 120

Query: 121 YGPYWRNIRKMCTLELLSSLKINSFSSMRKQEVGSLIKGLEDAATDGVAVDLTSKISSVI 180
           YGPYWRNIRKMCTLELLSSLKINSFSSMRKQEVGSLIKGLEDAATDGVAVDLTSKISSVI
Sbjct: 121 YGPYWRNIRKMCTLELLSSLKINSFSSMRKQEVGSLIKGLEDAATDGVAVDLTSKISSVI 180

Query: 181 GDMICVMVLGRKYEDNELGEKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKA 240
           GDMICVMVLGRKYEDNELGEKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKA
Sbjct: 181 GDMICVMVLGRKYEDNELGEKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKA 240

Query: 241 VGKIFDGFLESIVEEHVVFEKDNKDKDFVDVLLDLMGSREYQIDRSNIKAIILDFVIAAV 300
           VGKIFDGFLESIVEEHVVFEKDNKDKDFVDVLLDLMGSREYQIDRSNIKAIILDFVIAAV
Sbjct: 241 VGKIFDGFLESIVEEHVVFEKDNKDKDFVDVLLDLMGSREYQIDRSNIKAIILDFVIAAV 300

Query: 301 DSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVMKESLRMH 360
           DSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVMKESLRMH
Sbjct: 301 DSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVMKESLRMH 360

Query: 361 PPVPLIPRECIQDCNINGYHIPKKSRIVINAWAIGRDPNTWVDPHKFDPERFLESEVDVR 420
           PPVPLIPRECIQDCNINGYHIPKKSRIVINAWAIGRDPNTWVDPHKFDPERFLESEVDVR
Sbjct: 361 PPVPLIPRECIQDCNINGYHIPKKSRIVINAWAIGRDPNTWVDPHKFDPERFLESEVDVR 420

Query: 421 GRDFELIPFGSGRRGCVGIQLALVVVRLVVAQLLHCFDLKLPDGMSPLELDMTEILGLTC 480
           GRDFELIPFGSGRRGCVGIQLALVVVRLVVAQLLHCFDLKLPDGMSPLELDMTEILGLTC
Sbjct: 421 GRDFELIPFGSGRRGCVGIQLALVVVRLVVAQLLHCFDLKLPDGMSPLELDMTEILGLTC 480

Query: 481 PRAQNLRVIPIFRLCPNYL 500
           PRAQNLRVIPIFRLCPNYL
Sbjct: 481 PRAQNLRVIPIFRLCPNYL 499

BLAST of IVF0010812 vs. ExPASy TrEMBL
Match: A0A1S3B2K5 (cytochrome P450 CYP736A12-like OS=Cucumis melo OX=3656 GN=LOC103485291 PE=3 SV=1)

HSP 1 Score: 998.4 bits (2580), Expect = 1.0e-287
Identity = 499/499 (100.00%), Postives = 499/499 (100.00%), Query Frame = 0

Query: 1   MTWIILATITLIFLLPYLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYL 60
           MTWIILATITLIFLLPYLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYL
Sbjct: 1   MTWIILATITLIFLLPYLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYL 60

Query: 61  SQIYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFASRPLTITSNHISYGRKGIVFAQ 120
           SQIYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFASRPLTITSNHISYGRKGIVFAQ
Sbjct: 61  SQIYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFASRPLTITSNHISYGRKGIVFAQ 120

Query: 121 YGPYWRNIRKMCTLELLSSLKINSFSSMRKQEVGSLIKGLEDAATDGVAVDLTSKISSVI 180
           YGPYWRNIRKMCTLELLSSLKINSFSSMRKQEVGSLIKGLEDAATDGVAVDLTSKISSVI
Sbjct: 121 YGPYWRNIRKMCTLELLSSLKINSFSSMRKQEVGSLIKGLEDAATDGVAVDLTSKISSVI 180

Query: 181 GDMICVMVLGRKYEDNELGEKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKA 240
           GDMICVMVLGRKYEDNELGEKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKA
Sbjct: 181 GDMICVMVLGRKYEDNELGEKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKA 240

Query: 241 VGKIFDGFLESIVEEHVVFEKDNKDKDFVDVLLDLMGSREYQIDRSNIKAIILDFVIAAV 300
           VGKIFDGFLESIVEEHVVFEKDNKDKDFVDVLLDLMGSREYQIDRSNIKAIILDFVIAAV
Sbjct: 241 VGKIFDGFLESIVEEHVVFEKDNKDKDFVDVLLDLMGSREYQIDRSNIKAIILDFVIAAV 300

Query: 301 DSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVMKESLRMH 360
           DSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVMKESLRMH
Sbjct: 301 DSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVMKESLRMH 360

Query: 361 PPVPLIPRECIQDCNINGYHIPKKSRIVINAWAIGRDPNTWVDPHKFDPERFLESEVDVR 420
           PPVPLIPRECIQDCNINGYHIPKKSRIVINAWAIGRDPNTWVDPHKFDPERFLESEVDVR
Sbjct: 361 PPVPLIPRECIQDCNINGYHIPKKSRIVINAWAIGRDPNTWVDPHKFDPERFLESEVDVR 420

Query: 421 GRDFELIPFGSGRRGCVGIQLALVVVRLVVAQLLHCFDLKLPDGMSPLELDMTEILGLTC 480
           GRDFELIPFGSGRRGCVGIQLALVVVRLVVAQLLHCFDLKLPDGMSPLELDMTEILGLTC
Sbjct: 421 GRDFELIPFGSGRRGCVGIQLALVVVRLVVAQLLHCFDLKLPDGMSPLELDMTEILGLTC 480

Query: 481 PRAQNLRVIPIFRLCPNYL 500
           PRAQNLRVIPIFRLCPNYL
Sbjct: 481 PRAQNLRVIPIFRLCPNYL 499

BLAST of IVF0010812 vs. ExPASy TrEMBL
Match: A0A5A7T3X6 (Cytochrome P450 CYP736A12-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold18G00600 PE=3 SV=1)

HSP 1 Score: 991.1 bits (2561), Expect = 1.7e-285
Identity = 496/499 (99.40%), Postives = 496/499 (99.40%), Query Frame = 0

Query: 1   MTWIILATITLIFLLPYLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYL 60
           MTWIILATITLIFLLPYLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYL
Sbjct: 1   MTWIILATITLIFLLPYLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYL 60

Query: 61  SQIYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFASRPLTITSNHISYGRKGIVFAQ 120
           SQIYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFASRPLTITSNHISYGRKGI FAQ
Sbjct: 61  SQIYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFASRPLTITSNHISYGRKGIAFAQ 120

Query: 121 YGPYWRNIRKMCTLELLSSLKINSFSSMRKQEVGSLIKGLEDAATDGVAVDLTSKISSVI 180
           YGPYWRNIRKMCTLELLSSLKINSFSS RKQEVGSLIKGLEDAATDGVAVDLTSKISSVI
Sbjct: 121 YGPYWRNIRKMCTLELLSSLKINSFSSTRKQEVGSLIKGLEDAATDGVAVDLTSKISSVI 180

Query: 181 GDMICVMVLGRKYEDNELGEKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKA 240
           GDMICVMVLGRKYEDNELGEKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKA
Sbjct: 181 GDMICVMVLGRKYEDNELGEKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKA 240

Query: 241 VGKIFDGFLESIVEEHVVFEKDNKDKDFVDVLLDLMGSREYQIDRSNIKAIILDFVIAAV 300
           VGKIFDGFLESIVEEHVVFEKDNKDKDFVDVLLDLMGSREYQIDRSNIKAIILDFVIAAV
Sbjct: 241 VGKIFDGFLESIVEEHVVFEKDNKDKDFVDVLLDLMGSREYQIDRSNIKAIILDFVIAAV 300

Query: 301 DSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVMKESLRMH 360
           DSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVMKESLRMH
Sbjct: 301 DSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVMKESLRMH 360

Query: 361 PPVPLIPRECIQDCNINGYHIPKKSRIVINAWAIGRDPNTWVDPHKFDPERFLESEVDVR 420
           PPVPLIPRECIQDCNINGYHIPKKSRIVINAWAIGRDPNTWVDPHKFDPERFLESEVDVR
Sbjct: 361 PPVPLIPRECIQDCNINGYHIPKKSRIVINAWAIGRDPNTWVDPHKFDPERFLESEVDVR 420

Query: 421 GRDFELIPFGSGRRGCVGIQLALVVVRLVVAQLLHCFDLKLPDGMSPLELDMTEILGLTC 480
           GRDFELIPFGSGRRGCVGIQLALVVVRLVVAQLLHCFDLKLPDGMSPLELDMTEILGLTC
Sbjct: 421 GRDFELIPFGSGRRGCVGIQLALVVVRLVVAQLLHCFDLKLPDGMSPLELDMTEILGLTC 480

Query: 481 PRAQNLRVIPIFRLCPNYL 500
           PRAQNLRVIPIFRLCPN L
Sbjct: 481 PRAQNLRVIPIFRLCPNNL 499

BLAST of IVF0010812 vs. ExPASy TrEMBL
Match: A0A6J1BYU0 (cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005927 PE=3 SV=1)

HSP 1 Score: 646.4 bits (1666), Expect = 1.0e-181
Identity = 315/497 (63.38%), Postives = 398/497 (80.08%), Query Frame = 0

Query: 1   MTWIILATITLIFLLPYLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYL 60
           M WI+LA + L  L   + + WL +++   NKLPPGP+GFP+ GSLHLLGKL H+DL  L
Sbjct: 1   MAWILLA-VALAGLALLILKPWLFRSQ---NKLPPGPRGFPVFGSLHLLGKLPHRDLRDL 60

Query: 61  SQIYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFASRPLTITSNHISYGRKGIVFAQ 120
           SQ YG IM+++LG++P IIV+S +A ELFLKTHDL FASRP    S ++SYG+K + FAQ
Sbjct: 61  SQKYGSIMYMRLGIVPTIIVTSPQAAELFLKTHDLVFASRPFFEASKYMSYGQKNLGFAQ 120

Query: 121 YGPYWRNIRKMCTLELLSSLKINSFSSMRKQEVGSLIKGLEDAATDGVAVDLTSKISSVI 180
           YG YWRN+RKMCTLELLS  K+NSF SMR++EVG L+  L DAA D V ++L+SKI+S+ 
Sbjct: 121 YGSYWRNMRKMCTLELLSHHKVNSFRSMRREEVGLLVHYLRDAAKDHVVINLSSKIASLN 180

Query: 181 GDMICVMVLGRKYEDNELGEKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKA 240
            DM C+MV G+KY D E  E+GFK +I+EA QL A PNLGDFIP IA  D+QG   R+KA
Sbjct: 181 TDMTCLMVFGKKYADAEFDERGFKSVIQEAMQLVATPNLGDFIPCIAALDLQGLNRRSKA 240

Query: 241 VGKIFDGFLESIVEEHVVFEKDNKDKDFVDVLLDLMGSR--EYQIDRSNIKAIILDFVIA 300
           + KIFDGF E I++EH+  + +NK KDFVDV+LD+MGS+  EYQIDRS+IKAIILD ++A
Sbjct: 241 ISKIFDGFFEKIIDEHLESKNENKTKDFVDVMLDIMGSQETEYQIDRSSIKAIILDMLVA 300

Query: 301 AVDSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVMKESLR 360
           A+D++ TT+ W ++ELIKHP +MKKLQEEL+KVVGL+R VEESDL +L+YL+M +KE LR
Sbjct: 301 AMDTSATTVGWAIAELIKHPQVMKKLQEELQKVVGLDRRVEESDLDHLEYLDMFVKEILR 360

Query: 361 MHPPVP-LIPRECIQDCNINGYHIPKKSRIVINAWAIGRDPNTWVDPHKFDPERFLESEV 420
           +HP  P L+P E ++DC +NGYHIPKKSRI++NAWAIG+DPN+WVDP KF PERF+ S+V
Sbjct: 361 VHPAAPLLVPHESLEDCTVNGYHIPKKSRIIVNAWAIGQDPNSWVDPEKFFPERFIGSQV 420

Query: 421 DVRGRDFELIPFGSGRRGCVGIQLALVVVRLVVAQLLHCFDLKLPDGMSPLELDMTEILG 480
           DV+GRDF+LIPFGSGRRGC G+ + LVVV  V+AQL+HCFD KLP+GM P ELDMTE  G
Sbjct: 421 DVKGRDFQLIPFGSGRRGCPGMHMGLVVVHFVIAQLVHCFDWKLPNGMLPAELDMTEEFG 480

Query: 481 LTCPRAQNLRVIPIFRL 495
           LTCPRAQ+L V PI+RL
Sbjct: 481 LTCPRAQDLMVTPIYRL 493

BLAST of IVF0010812 vs. ExPASy TrEMBL
Match: A0A6J1BTE3 (cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005393 PE=3 SV=1)

HSP 1 Score: 643.7 bits (1659), Expect = 6.5e-181
Identity = 318/495 (64.24%), Postives = 399/495 (80.61%), Query Frame = 0

Query: 3   WIILATITLIFLLPYLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYLSQ 62
           W+I+ TI  +FL   LF  WL   +    +LPPGPKGFPI GSLHLLGKL H+ LH LSQ
Sbjct: 5   WLII-TILPLFL---LFSSWLWVLRSHA-RLPPGPKGFPIFGSLHLLGKLPHRALHRLSQ 64

Query: 63  IYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFASRPLTITSNHISYGRKGIVFAQYG 122
            YGPIMH++LGL+  I+VSS  A ELFLKTHD  FASRPL   SNHISYGRKG+ FA+YG
Sbjct: 65  KYGPIMHLRLGLVSTIVVSSPAAAELFLKTHDHVFASRPLIQASNHISYGRKGLGFAEYG 124

Query: 123 PYWRNIRKMCTLELLSSLKINSFSSMRKQEVGSLIKGLEDAATDGVAVDLTSKISSVIGD 182
           PYWRNIRKMCT+ELLS+LK++SF SMR +EVG LI  L +AA   V V+L+SK++S++ D
Sbjct: 125 PYWRNIRKMCTVELLSNLKVSSFRSMRSEEVGLLIDHLREAAKSDVVVNLSSKVTSLVAD 184

Query: 183 MICVMVLGRKYEDNELGEKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKAVG 242
           M C+MV G+KY D +L E+GFK +I+E  QLAAAPNLGDFIP +A  D QGF  RAK++ 
Sbjct: 185 MTCLMVFGKKYGDEQLDERGFKAVIQEVMQLAAAPNLGDFIPYVAGLDFQGFNRRAKSIS 244

Query: 243 KIFDGFLESIVEEHVVFEKDNKDKDFVDVLLDLMGSR--EYQIDRSNIKAIILDFVIAAV 302
           K+ DGFLE I++EH+  +  NK KDFVDV+LDLM S+  EYQI+RS+IKAIILD + AAV
Sbjct: 245 KVIDGFLERIIDEHLQPKIGNKTKDFVDVMLDLMNSQATEYQIERSSIKAIILDLLAAAV 304

Query: 303 DSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVMKESLRMH 362
           D++ TTI+W LSELIKHP  MKK+Q+ELEKVVGL+RMVE+SDL NLKYL+MV+KE  RMH
Sbjct: 305 DTSATTISWALSELIKHPLSMKKIQDELEKVVGLDRMVEDSDLCNLKYLDMVIKEIFRMH 364

Query: 363 PPVP-LIPRECIQDCNINGYHIPKKSRIVINAWAIGRDPNTWVDPHKFDPERFLESEVDV 422
           PP P L+P E + DC ++ ++IPKKSRI++NAWAIGRDPN W+D  KF PERF+ S+VD+
Sbjct: 365 PPAPLLVPHESLDDCTVDNFYIPKKSRIIVNAWAIGRDPNVWIDAEKFFPERFIGSQVDI 424

Query: 423 RGRDFELIPFGSGRRGCVGIQLALVVVRLVVAQLLHCFDLKLPDGMSPLELDMTEILGLT 482
           +GRDF+LIPFGSGRRGC G+Q+ L+VVRLV+AQL+HCF+ +LP+G  P +L+MTE  GLT
Sbjct: 425 KGRDFQLIPFGSGRRGCPGMQMGLIVVRLVLAQLVHCFNWELPNGTLPTKLNMTEEFGLT 484

Query: 483 CPRAQNLRVIPIFRL 495
           CPRA++L+VIPI+R+
Sbjct: 485 CPRAEDLKVIPIYRI 494

BLAST of IVF0010812 vs. NCBI nr
Match: XP_008441066.1 (PREDICTED: cytochrome P450 CYP736A12-like [Cucumis melo] >TYK12655.1 cytochrome P450 CYP736A12-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 998 bits (2580), Expect = 0.0
Identity = 499/499 (100.00%), Postives = 499/499 (100.00%), Query Frame = 0

Query: 1   MTWIILATITLIFLLPYLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYL 60
           MTWIILATITLIFLLPYLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYL
Sbjct: 1   MTWIILATITLIFLLPYLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYL 60

Query: 61  SQIYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFASRPLTITSNHISYGRKGIVFAQ 120
           SQIYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFASRPLTITSNHISYGRKGIVFAQ
Sbjct: 61  SQIYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFASRPLTITSNHISYGRKGIVFAQ 120

Query: 121 YGPYWRNIRKMCTLELLSSLKINSFSSMRKQEVGSLIKGLEDAATDGVAVDLTSKISSVI 180
           YGPYWRNIRKMCTLELLSSLKINSFSSMRKQEVGSLIKGLEDAATDGVAVDLTSKISSVI
Sbjct: 121 YGPYWRNIRKMCTLELLSSLKINSFSSMRKQEVGSLIKGLEDAATDGVAVDLTSKISSVI 180

Query: 181 GDMICVMVLGRKYEDNELGEKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKA 240
           GDMICVMVLGRKYEDNELGEKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKA
Sbjct: 181 GDMICVMVLGRKYEDNELGEKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKA 240

Query: 241 VGKIFDGFLESIVEEHVVFEKDNKDKDFVDVLLDLMGSREYQIDRSNIKAIILDFVIAAV 300
           VGKIFDGFLESIVEEHVVFEKDNKDKDFVDVLLDLMGSREYQIDRSNIKAIILDFVIAAV
Sbjct: 241 VGKIFDGFLESIVEEHVVFEKDNKDKDFVDVLLDLMGSREYQIDRSNIKAIILDFVIAAV 300

Query: 301 DSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVMKESLRMH 360
           DSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVMKESLRMH
Sbjct: 301 DSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVMKESLRMH 360

Query: 361 PPVPLIPRECIQDCNINGYHIPKKSRIVINAWAIGRDPNTWVDPHKFDPERFLESEVDVR 420
           PPVPLIPRECIQDCNINGYHIPKKSRIVINAWAIGRDPNTWVDPHKFDPERFLESEVDVR
Sbjct: 361 PPVPLIPRECIQDCNINGYHIPKKSRIVINAWAIGRDPNTWVDPHKFDPERFLESEVDVR 420

Query: 421 GRDFELIPFGSGRRGCVGIQLALVVVRLVVAQLLHCFDLKLPDGMSPLELDMTEILGLTC 480
           GRDFELIPFGSGRRGCVGIQLALVVVRLVVAQLLHCFDLKLPDGMSPLELDMTEILGLTC
Sbjct: 421 GRDFELIPFGSGRRGCVGIQLALVVVRLVVAQLLHCFDLKLPDGMSPLELDMTEILGLTC 480

Query: 481 PRAQNLRVIPIFRLCPNYL 499
           PRAQNLRVIPIFRLCPNYL
Sbjct: 481 PRAQNLRVIPIFRLCPNYL 499

BLAST of IVF0010812 vs. NCBI nr
Match: KAA0036261.1 (cytochrome P450 CYP736A12-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 991 bits (2561), Expect = 0.0
Identity = 496/499 (99.40%), Postives = 496/499 (99.40%), Query Frame = 0

Query: 1   MTWIILATITLIFLLPYLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYL 60
           MTWIILATITLIFLLPYLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYL
Sbjct: 1   MTWIILATITLIFLLPYLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYL 60

Query: 61  SQIYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFASRPLTITSNHISYGRKGIVFAQ 120
           SQIYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFASRPLTITSNHISYGRKGI FAQ
Sbjct: 61  SQIYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFASRPLTITSNHISYGRKGIAFAQ 120

Query: 121 YGPYWRNIRKMCTLELLSSLKINSFSSMRKQEVGSLIKGLEDAATDGVAVDLTSKISSVI 180
           YGPYWRNIRKMCTLELLSSLKINSFSS RKQEVGSLIKGLEDAATDGVAVDLTSKISSVI
Sbjct: 121 YGPYWRNIRKMCTLELLSSLKINSFSSTRKQEVGSLIKGLEDAATDGVAVDLTSKISSVI 180

Query: 181 GDMICVMVLGRKYEDNELGEKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKA 240
           GDMICVMVLGRKYEDNELGEKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKA
Sbjct: 181 GDMICVMVLGRKYEDNELGEKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKA 240

Query: 241 VGKIFDGFLESIVEEHVVFEKDNKDKDFVDVLLDLMGSREYQIDRSNIKAIILDFVIAAV 300
           VGKIFDGFLESIVEEHVVFEKDNKDKDFVDVLLDLMGSREYQIDRSNIKAIILDFVIAAV
Sbjct: 241 VGKIFDGFLESIVEEHVVFEKDNKDKDFVDVLLDLMGSREYQIDRSNIKAIILDFVIAAV 300

Query: 301 DSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVMKESLRMH 360
           DSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVMKESLRMH
Sbjct: 301 DSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVMKESLRMH 360

Query: 361 PPVPLIPRECIQDCNINGYHIPKKSRIVINAWAIGRDPNTWVDPHKFDPERFLESEVDVR 420
           PPVPLIPRECIQDCNINGYHIPKKSRIVINAWAIGRDPNTWVDPHKFDPERFLESEVDVR
Sbjct: 361 PPVPLIPRECIQDCNINGYHIPKKSRIVINAWAIGRDPNTWVDPHKFDPERFLESEVDVR 420

Query: 421 GRDFELIPFGSGRRGCVGIQLALVVVRLVVAQLLHCFDLKLPDGMSPLELDMTEILGLTC 480
           GRDFELIPFGSGRRGCVGIQLALVVVRLVVAQLLHCFDLKLPDGMSPLELDMTEILGLTC
Sbjct: 421 GRDFELIPFGSGRRGCVGIQLALVVVRLVVAQLLHCFDLKLPDGMSPLELDMTEILGLTC 480

Query: 481 PRAQNLRVIPIFRLCPNYL 499
           PRAQNLRVIPIFRLCPN L
Sbjct: 481 PRAQNLRVIPIFRLCPNNL 499

BLAST of IVF0010812 vs. NCBI nr
Match: XP_023543453.1 (cytochrome P450 CYP736A12-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 711 bits (1835), Expect = 1.29e-253
Identity = 358/493 (72.62%), Postives = 415/493 (84.18%), Query Frame = 0

Query: 4   IILATITLIFLLPYLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYLSQI 63
           +ILAT+ LI LL YL Q W+ K+    NKLPPGPKGFPIIGSLHLL KLIH+DLH+LSQ 
Sbjct: 3   LILATMILISLLTYLIQGWVFKSNTFGNKLPPGPKGFPIIGSLHLLSKLIHRDLHHLSQR 62

Query: 64  YGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFASRPLTITSNHISYGRKGIVFAQYGP 123
           YGPIM++QLG + AI+VSS RA ELFLKTHDL F SRPLT TSN ISYGRKGI F+QY  
Sbjct: 63  YGPIMYMQLGRVHAIVVSSPRAAELFLKTHDLDFPSRPLTKTSNQISYGRKGIAFSQYDS 122

Query: 124 YWRNIRKMCTLELLSSLKINSFSSMRKQEVGSLIKGLEDAATDGVAVDLTSKISSVIGDM 183
           +WRN+RKMCTLEL S LKINSFSSMRKQEV  L+  L++AA + VAVDL+SKIS +I D+
Sbjct: 123 HWRNMRKMCTLELFSFLKINSFSSMRKQEVRLLVDDLQEAAENRVAVDLSSKISCLIVDI 182

Query: 184 ICVMVLGRKYEDNELGEKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKAVGK 243
           +C+MV+GRKY D EL  KGFK +I+EAT+LAAAPNLGDF P IAR D+QG   RAK+VG 
Sbjct: 183 MCLMVVGRKYRDKELDAKGFKAVIQEATRLAAAPNLGDFFPFIARLDLQGLCRRAKSVGD 242

Query: 244 IFDGFLESIVEEHVVFEKDNKDKDFVDVLLDLMGSRE--YQIDRSNIKAIILDFVIAAVD 303
           +FDGFLE IVEEHVV  K++K+KDFVDV+LDLMGSRE  YQID S IKA+ILD +IAAVD
Sbjct: 243 VFDGFLEKIVEEHVV-SKNDKNKDFVDVMLDLMGSRENDYQIDCSTIKAMILDLLIAAVD 302

Query: 304 STTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVMKESLRMHP 363
           S+TT INW L ELIKHPHIMKK+QEELEKVVGLNRMVEESDL+NLKYLEMV+KE+ RMHP
Sbjct: 303 SSTTIINWALPELIKHPHIMKKMQEELEKVVGLNRMVEESDLNNLKYLEMVIKETFRMHP 362

Query: 364 PVPLIPRECIQDCNINGYHIPKKSRIVINAWAIGRDPNTWVDPHKFDPERFLESEVDVRG 423
           P+PLIPRE IQDC +NGYHIP KSR+VINAWAIGRD  TW+DP +F PERF++ +VD++G
Sbjct: 363 PIPLIPRESIQDCMVNGYHIPNKSRLVINAWAIGRDARTWLDPDEFIPERFVDEQVDMKG 422

Query: 424 RDFELIPFGSGRRGCVGIQLALVVVRLVVAQLLHCFDLKLPDGMSPLELDMTEILGLTCP 483
           +DF+LIPFG GRRGC G QLAL  VRLVVAQL+HCFD +LP+GM   ELDMTE  GLTCP
Sbjct: 423 KDFKLIPFGYGRRGCPGTQLALTTVRLVVAQLVHCFDWELPNGMMQAELDMTEEFGLTCP 482

Query: 484 RAQNLRVIPIFRL 494
           RAQNL +IP +RL
Sbjct: 483 RAQNLVLIPRYRL 494

BLAST of IVF0010812 vs. NCBI nr
Match: XP_022133318.1 (cytochrome P450 CYP736A12-like [Momordica charantia])

HSP 1 Score: 646 bits (1666), Expect = 5.33e-228
Identity = 315/497 (63.38%), Postives = 398/497 (80.08%), Query Frame = 0

Query: 1   MTWIILATITLIFLLPYLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYL 60
           M WI+LA + L  L   + + WL +++   NKLPPGP+GFP+ GSLHLLGKL H+DL  L
Sbjct: 1   MAWILLA-VALAGLALLILKPWLFRSQ---NKLPPGPRGFPVFGSLHLLGKLPHRDLRDL 60

Query: 61  SQIYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFASRPLTITSNHISYGRKGIVFAQ 120
           SQ YG IM+++LG++P IIV+S +A ELFLKTHDL FASRP    S ++SYG+K + FAQ
Sbjct: 61  SQKYGSIMYMRLGIVPTIIVTSPQAAELFLKTHDLVFASRPFFEASKYMSYGQKNLGFAQ 120

Query: 121 YGPYWRNIRKMCTLELLSSLKINSFSSMRKQEVGSLIKGLEDAATDGVAVDLTSKISSVI 180
           YG YWRN+RKMCTLELLS  K+NSF SMR++EVG L+  L DAA D V ++L+SKI+S+ 
Sbjct: 121 YGSYWRNMRKMCTLELLSHHKVNSFRSMRREEVGLLVHYLRDAAKDHVVINLSSKIASLN 180

Query: 181 GDMICVMVLGRKYEDNELGEKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKA 240
            DM C+MV G+KY D E  E+GFK +I+EA QL A PNLGDFIP IA  D+QG   R+KA
Sbjct: 181 TDMTCLMVFGKKYADAEFDERGFKSVIQEAMQLVATPNLGDFIPCIAALDLQGLNRRSKA 240

Query: 241 VGKIFDGFLESIVEEHVVFEKDNKDKDFVDVLLDLMGSRE--YQIDRSNIKAIILDFVIA 300
           + KIFDGF E I++EH+  + +NK KDFVDV+LD+MGS+E  YQIDRS+IKAIILD ++A
Sbjct: 241 ISKIFDGFFEKIIDEHLESKNENKTKDFVDVMLDIMGSQETEYQIDRSSIKAIILDMLVA 300

Query: 301 AVDSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVMKESLR 360
           A+D++ TT+ W ++ELIKHP +MKKLQEEL+KVVGL+R VEESDL +L+YL+M +KE LR
Sbjct: 301 AMDTSATTVGWAIAELIKHPQVMKKLQEELQKVVGLDRRVEESDLDHLEYLDMFVKEILR 360

Query: 361 MHPPVPL-IPRECIQDCNINGYHIPKKSRIVINAWAIGRDPNTWVDPHKFDPERFLESEV 420
           +HP  PL +P E ++DC +NGYHIPKKSRI++NAWAIG+DPN+WVDP KF PERF+ S+V
Sbjct: 361 VHPAAPLLVPHESLEDCTVNGYHIPKKSRIIVNAWAIGQDPNSWVDPEKFFPERFIGSQV 420

Query: 421 DVRGRDFELIPFGSGRRGCVGIQLALVVVRLVVAQLLHCFDLKLPDGMSPLELDMTEILG 480
           DV+GRDF+LIPFGSGRRGC G+ + LVVV  V+AQL+HCFD KLP+GM P ELDMTE  G
Sbjct: 421 DVKGRDFQLIPFGSGRRGCPGMHMGLVVVHFVIAQLVHCFDWKLPNGMLPAELDMTEEFG 480

Query: 481 LTCPRAQNLRVIPIFRL 494
           LTCPRAQ+L V PI+RL
Sbjct: 481 LTCPRAQDLMVTPIYRL 493

BLAST of IVF0010812 vs. NCBI nr
Match: XP_022132564.1 (cytochrome P450 CYP736A12-like [Momordica charantia])

HSP 1 Score: 643 bits (1658), Expect = 8.75e-227
Identity = 318/495 (64.24%), Postives = 399/495 (80.61%), Query Frame = 0

Query: 3   WIILATITLIFLLPYLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYLSQ 62
           W+I+ TI  +FLL   F  WL   +    +LPPGPKGFPI GSLHLLGKL H+ LH LSQ
Sbjct: 5   WLII-TILPLFLL---FSSWLWVLRSHA-RLPPGPKGFPIFGSLHLLGKLPHRALHRLSQ 64

Query: 63  IYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFASRPLTITSNHISYGRKGIVFAQYG 122
            YGPIMH++LGL+  I+VSS  A ELFLKTHD  FASRPL   SNHISYGRKG+ FA+YG
Sbjct: 65  KYGPIMHLRLGLVSTIVVSSPAAAELFLKTHDHVFASRPLIQASNHISYGRKGLGFAEYG 124

Query: 123 PYWRNIRKMCTLELLSSLKINSFSSMRKQEVGSLIKGLEDAATDGVAVDLTSKISSVIGD 182
           PYWRNIRKMCT+ELLS+LK++SF SMR +EVG LI  L +AA   V V+L+SK++S++ D
Sbjct: 125 PYWRNIRKMCTVELLSNLKVSSFRSMRSEEVGLLIDHLREAAKSDVVVNLSSKVTSLVAD 184

Query: 183 MICVMVLGRKYEDNELGEKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKAVG 242
           M C+MV G+KY D +L E+GFK +I+E  QLAAAPNLGDFIP +A  D QGF  RAK++ 
Sbjct: 185 MTCLMVFGKKYGDEQLDERGFKAVIQEVMQLAAAPNLGDFIPYVAGLDFQGFNRRAKSIS 244

Query: 243 KIFDGFLESIVEEHVVFEKDNKDKDFVDVLLDLMGSR--EYQIDRSNIKAIILDFVIAAV 302
           K+ DGFLE I++EH+  +  NK KDFVDV+LDLM S+  EYQI+RS+IKAIILD + AAV
Sbjct: 245 KVIDGFLERIIDEHLQPKIGNKTKDFVDVMLDLMNSQATEYQIERSSIKAIILDLLAAAV 304

Query: 303 DSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVMKESLRMH 362
           D++ TTI+W LSELIKHP  MKK+Q+ELEKVVGL+RMVE+SDL NLKYL+MV+KE  RMH
Sbjct: 305 DTSATTISWALSELIKHPLSMKKIQDELEKVVGLDRMVEDSDLCNLKYLDMVIKEIFRMH 364

Query: 363 PPVPL-IPRECIQDCNINGYHIPKKSRIVINAWAIGRDPNTWVDPHKFDPERFLESEVDV 422
           PP PL +P E + DC ++ ++IPKKSRI++NAWAIGRDPN W+D  KF PERF+ S+VD+
Sbjct: 365 PPAPLLVPHESLDDCTVDNFYIPKKSRIIVNAWAIGRDPNVWIDAEKFFPERFIGSQVDI 424

Query: 423 RGRDFELIPFGSGRRGCVGIQLALVVVRLVVAQLLHCFDLKLPDGMSPLELDMTEILGLT 482
           +GRDF+LIPFGSGRRGC G+Q+ L+VVRLV+AQL+HCF+ +LP+G  P +L+MTE  GLT
Sbjct: 425 KGRDFQLIPFGSGRRGCPGMQMGLIVVRLVLAQLVHCFNWELPNGTLPTKLNMTEEFGLT 484

Query: 483 CPRAQNLRVIPIFRL 494
           CPRA++L+VIPI+R+
Sbjct: 485 CPRAEDLKVIPIYRI 494

BLAST of IVF0010812 vs. TAIR 10
Match: AT5G07990.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 370.9 bits (951), Expect = 1.6e-102
Identity = 204/505 (40.40%), Postives = 306/505 (60.59%), Query Frame = 0

Query: 4   IILATITLIFLLPYLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYLSQI 63
           I+LAT+  +FL+  +F     + +   N+LPPGP  +PIIG+L  +G   H+ L  +   
Sbjct: 8   ILLATV--LFLILRIFSH--RRNRSHNNRLPPGPNPWPIIGNLPHMGTKPHRTLSAMVTT 67

Query: 64  YGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFASRPLTITSNHISYGRKGIVFAQYGP 123
           YGPI+H++LG +  ++ +S    E FLK HD +FASRP    + H++Y  + +VFA YG 
Sbjct: 68  YGPILHLRLGFVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVFAPYGH 127

Query: 124 YWRNIRKMCTLELLSSLKINSFSSMRKQEVGSLIKGLEDAATDGVAVDLTSKISSVIGDM 183
            WR +RK+ ++ L S+  +  F  +R++EVG+L + L    T    V+L   ++  + + 
Sbjct: 128 RWRLLRKISSVHLFSAKALEDFKHVRQEEVGTLTRELVRVGTK--PVNLGQLVNMCVVNA 187

Query: 184 ICVMVLGRKYEDNELGEKG--FKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKAV 243
           +   ++GR+    +   K   F+ ++ E   LA   N+GDF+P +   D+QG  G+ K +
Sbjct: 188 LGREMIGRRLFGADADHKADEFRSMVTEMMALAGVFNIGDFVPSLDWLDLQGVAGKMKRL 247

Query: 244 GKIFDGFLESIVEEHVVFEKDNKDKDFVDVLLDLMGS----REYQIDRSNIKAIILDFVI 303
            K FD FL SI++EH +  +D K  D +  L+ L G+        +  + IKA++L+   
Sbjct: 248 HKRFDAFLSSILKEHEMNGQDQKHTDMLSTLISLKGTDLDGDGGSLTDTEIKALLLNMFT 307

Query: 304 AAVDSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVMKESL 363
           A  D++ +T++W ++ELI+HP IM K QEEL+ VVG +R V ESD++ L YL+ V+KE+ 
Sbjct: 308 AGTDTSASTVDWAIAELIRHPDIMVKAQEELDIVVGRDRPVNESDIAQLPYLQAVIKENF 367

Query: 364 RMHPPVPL-IPRECIQDCNINGYHIPKKSRIVINAWAIGRDPNTWVDPHKFDPERFL--- 423
           R+HPP PL +P    + C INGYHIPK S ++ N WAI RDP+ W DP  F PERFL   
Sbjct: 368 RLHPPTPLSLPHIASESCEINGYHIPKGSTLLTNIWAIARDPDQWSDPLAFKPERFLPGG 427

Query: 424 -ESEVDVRGRDFELIPFGSGRRGCVGIQLALVVVRLVVAQLLHCFDLKLPDGMSPLELDM 483
            +S VDV+G DFELIPFG+GRR C G+ L L  ++ + A L+  FD +L  G++P +L+M
Sbjct: 428 EKSGVDVKGSDFELIPFGAGRRICAGLSLGLRTIQFLTATLVQGFDWELAGGVTPEKLNM 487

Query: 484 TEILGLTCPRAQNLRVIPIFRLCPN 498
            E  GLT  RA  L V P  RL PN
Sbjct: 488 EESYGLTLQRAVPLVVHPKPRLAPN 506

BLAST of IVF0010812 vs. TAIR 10
Match: AT4G36220.1 (ferulic acid 5-hydroxylase 1 )

HSP 1 Score: 366.3 bits (939), Expect = 3.9e-101
Identity = 195/484 (40.29%), Postives = 292/484 (60.33%), Query Frame = 0

Query: 34  PPGPKGFPIIGSLHLLGKLIHKDLHYLSQIYGPIMHIQLGLLPAIIVSSARATELFLKTH 93
           PPGP+G+PIIG++ ++ +L H+ L  L++ YG + H+++G L    VSS       L+  
Sbjct: 41  PPGPRGWPIIGNMLMMDQLTHRGLANLAKKYGGLCHLRMGFLHMYAVSSPEVARQVLQVQ 100

Query: 94  DLHFASRPLTITSNHISYGRKGIVFAQYGPYWRNIRKMCTLELLSSLKINSFSSMRKQEV 153
           D  F++RP TI  ++++Y R  + FA YGP+WR +RK+C +++ S  +  S++S+R  EV
Sbjct: 101 DSVFSNRPATIAISYLTYDRADMAFAHYGPFWRQMRKVCVMKVFSRKRAESWASVR-DEV 160

Query: 154 GSLIKGLEDAATDGVAVDLTSKISSVIGDMICVMVLGRKYEDNELGEKGFKGLIREATQL 213
             +++ +  +   G  +++  +I ++  ++      G   E    G+  F  +++E ++L
Sbjct: 161 DKMVRSV--SCNVGKPINVGEQIFALTRNITYRAAFGSACEK---GQDEFIRILQEFSKL 220

Query: 214 AAAPNLGDFIPLIARFDVQGFGGRAKAVGKIFDGFLESIVEEHV--------VFEKDNKD 273
             A N+ DFIP     D QG   R        DGF++ I++EH+        V + D  D
Sbjct: 221 FGAFNVADFIPYFGWIDPQGINKRLVKARNDLDGFIDDIIDEHMKKKENQNAVDDGDVVD 280

Query: 274 KDFVDVLL--------------DLMGSREYQIDRSNIKAIILDFVIAAVDSTTTTINWML 333
            D VD LL              DL  S   ++ R NIKAII+D +    ++  + I W L
Sbjct: 281 TDMVDDLLAFYSEEAKLVSETADLQNS--IKLTRDNIKAIIMDVMFGGTETVASAIEWAL 340

Query: 334 SELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVMKESLRMHPPVPLIPRECI 393
           +EL++ P  +K++Q+EL +VVGL+R VEESD+  L YL+  +KE+LRMHPP+PL+  E  
Sbjct: 341 TELLRSPEDLKRVQQELAEVVGLDRRVEESDIEKLTYLKCTLKETLRMHPPIPLLLHETA 400

Query: 394 QDCNINGYHIPKKSRIVINAWAIGRDPNTWVDPHKFDPERFLESEV-DVRGRDFELIPFG 453
           +D +I+G+ IPKKSR++INA+AIGRDP +W DP  F P RFLE  V D +G +FE IPFG
Sbjct: 401 EDTSIDGFFIPKKSRVMINAFAIGRDPTSWTDPDTFRPSRFLEPGVPDFKGSNFEFIPFG 460

Query: 454 SGRRGCVGIQLALVVVRLVVAQLLHCFDLKLPDGMSPLELDMTEILGLTCPRAQNLRVIP 495
           SGRR C G+QL L  + L VA +LHCF  KLPDGM P ELDM ++ GLT P+A  L  +P
Sbjct: 461 SGRRSCPGMQLGLYALDLAVAHILHCFTWKLPDGMKPSELDMNDVFGLTAPKATRLFAVP 516

BLAST of IVF0010812 vs. TAIR 10
Match: AT1G13080.1 (cytochrome P450, family 71, subfamily B, polypeptide 2 )

HSP 1 Score: 355.9 bits (912), Expect = 5.2e-98
Identity = 187/501 (37.33%), Postives = 301/501 (60.08%), Query Frame = 0

Query: 5   ILATITLIFLLPYLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYLSQIY 64
           IL    L+ LL  +   +L + K     LPP P   PIIG+LH L  L H+  H LS  Y
Sbjct: 3   ILLCFFLVSLLTIVSSIFLKQNKTSKFNLPPSPSSLPIIGNLHHLAGLPHRCFHKLSIKY 62

Query: 65  GPIMHIQLGLLPAIIVSSARATELFLKTHDLHFASRPLTITSNHISYGRKGIVFAQYGPY 124
           GP++ ++LG +P +++SS+ A E  LKT+DL   SRP T+ S  +SYG K I FA YG Y
Sbjct: 63  GPLVFLRLGSVPVVVISSSEAAEAVLKTNDLECCSRPKTVGSGKLSYGFKDITFAPYGEY 122

Query: 125 WRNIRKMCTLELLSSLKINSFSSMRKQEVGSLIKGLEDAATDGVAVDLTSKISSVIGDMI 184
           WR +RK+  +EL SS K+ SF  +R++EV  ++K + ++A     VDL+    S+   +I
Sbjct: 123 WREVRKLAVIELFSSKKVQSFRYIREEEVDFVVKKVSESALKQSPVDLSKTFFSLTASII 182

Query: 185 CVMVLGRKYEDN--ELGEKGFKGLIREATQLAAAPNLGDFIP-LIARFDVQGFGGRAKAV 244
           C + LG+ + ++   + +   + L+ E+ +        DF P  + RF V     R K +
Sbjct: 183 CRVALGQNFNESGFVIDQDRIEELVTESAEALGTFTFSDFFPGGLGRF-VDWLFQRHKKI 242

Query: 245 GKIF---DGFLESIVEEHVVFEKDNKDKDFVDVLLDLMGSRE----YQIDRSNIKAIILD 304
            K+F   D F + ++++H+  E   K++D V ++LD++  +E    ++++  N+KAI++D
Sbjct: 243 NKVFKELDAFYQHVIDDHLKPE-GRKNQDIVTLILDMIDKQEDSDSFKLNMDNLKAIVMD 302

Query: 305 FVIAAVDSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNR-MVEESDLSNLKYLEMVM 364
             +A +D++  T+ W ++ELI++P +MKK QE +   +GL +  + E DL  ++YL  ++
Sbjct: 303 VFLAGIDTSAVTMIWAMTELIRNPRVMKKAQESIRTTLGLKKERITEEDLGKVEYLNHIL 362

Query: 365 KESLRMHPPVP-LIPRECIQDCNINGYHIPKKSRIVINAWAIGRDPNTWVDPHKFDPERF 424
           KE+ R+HP +P ++PRE +    I GY IP K++I +N W IGRDP  W DP +F+PERF
Sbjct: 363 KETFRLHPALPFVVPRETMSHIKIQGYDIPPKTQIQLNVWTIGRDPKRWNDPEEFNPERF 422

Query: 425 LESEVDVRGRDFELIPFGSGRRGCVGIQLALVVVRLVVAQLLHCFDLKLPDGMSPLELDM 484
             S VD RG+ F+L+PFGSGRR C G+ +A+  V L +  LL+ FD  +PDG    ++DM
Sbjct: 423 ANSSVDFRGQHFDLLPFGSGRRICPGMPMAIASVELALMNLLYYFDWSMPDGTKGEDIDM 482

Query: 485 TEILGLTCPRAQNLRVIPIFR 494
            E   ++  +   L+++P+ R
Sbjct: 483 EEAGNISIVKKIPLQLVPVQR 501

BLAST of IVF0010812 vs. TAIR 10
Match: AT3G26310.1 (cytochrome P450, family 71, subfamily B, polypeptide 35 )

HSP 1 Score: 349.7 bits (896), Expect = 3.8e-96
Identity = 183/492 (37.20%), Postives = 293/492 (59.55%), Query Frame = 0

Query: 9   ITLIFLLPYLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYLSQIYGPIM 68
           + LIFL+  L   +  K  P   +  P P GFPIIG+LH +G+L H+ L  LS+ YGP+M
Sbjct: 7   LPLIFLVCILLAVFNHKKHPKYRQF-PCPPGFPIIGNLHQIGELPHQTLWKLSKKYGPVM 66

Query: 69  HIQLGLLPAIIVSSARATELFLKTHDLHFASRPLTITSNHISYGRKGIVFAQYGPYWRNI 128
           H+ LG +P ++VSS+      L+ HDLH  +RP       +SY    I F+ Y  YW+ +
Sbjct: 67  HLMLGRVPTVVVSSSDTARQVLRVHDLHCCTRPSLSGPRELSYNYLDIAFSPYDDYWKEV 126

Query: 129 RKMCTLELLSSLKINSFSSMRKQEVGSLIKGLEDAATDGVAVDLTSKISSVIGDMICVMV 188
           RK+C  EL S+ +++S   ++ +EV  +I  + ++A+    V+L +K   +   ++C   
Sbjct: 127 RKLCVQELFSTKQVHSIQPIKDEEVKKMIDSIAESASQKNPVNLNNKCLELTVSVVCRTA 186

Query: 189 LGRKYEDNELGEKGFKGLIREATQLAAAPNLGDFIPLIA-RFDV-QGFGGRAKAVGKIFD 248
            G  +E   L    F  ++REA ++  + +  DFIP +    DV  G  GR +   +  +
Sbjct: 187 FGVSFEGTVLNSDRFNKIVREALEMLGSFSAADFIPYVGWIIDVLTGLQGRRERSKRDLN 246

Query: 249 GFLESIVEEHVVFEKDNKDKDFVDVLLDLMGSREY----QIDRSNIKAIILDFVIAAVDS 308
            F E + + H   +K+  ++DFVD+LL L          ++ R++IKAI+LD ++A +D+
Sbjct: 247 AFFEQMFDLHKEGKKEG-NEDFVDLLLRLEKEEAVLGNDKLTRNHIKAILLDVLLAGIDT 306

Query: 309 TTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVMKESLRMHPP 368
           +  T+ W ++EL ++P +MKK+Q E+   +G   M+   D+  L+YL+MV+KE+ R+HP 
Sbjct: 307 SAITMTWAMTELARNPRVMKKVQSEIRTQMGNRSMISFEDMDQLEYLKMVIKETWRLHPT 366

Query: 369 VP-LIPRECIQDCNINGYHIPKKSRIVINAWAIGRDPNTWVDPHKFDPERFLESEVDVRG 428
            P L+PRE + + +INGY IP K+R+ +N WAIGRDP+TW DP  F PERF+++ +D +G
Sbjct: 367 TPLLLPREAMSEFDINGYTIPVKTRLHVNVWAIGRDPDTWKDPEVFLPERFMDNNIDAKG 426

Query: 429 RDFELIPFGSGRRGCVGIQLALVVVRLVVAQLLHCFDLKLPDGMSPLELDMTEILGLTCP 488
           + FEL+PFG GRR C  I +   +V   +A LL+ FD KLP+G+   ++D+ E  GLT  
Sbjct: 427 QHFELLPFGGGRRICPAIYMGTTMVEFGLANLLYHFDWKLPEGVEVKDIDVEEAPGLTVN 486

Query: 489 RAQNLRVIPIFR 494
           +   L ++P  R
Sbjct: 487 KKNELLLVPEMR 496

BLAST of IVF0010812 vs. TAIR 10
Match: AT3G26300.1 (cytochrome P450, family 71, subfamily B, polypeptide 34 )

HSP 1 Score: 349.0 bits (894), Expect = 6.4e-96
Identity = 183/495 (36.97%), Postives = 289/495 (58.38%), Query Frame = 0

Query: 9   ITLIFLLPYLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYLSQIYGPIM 68
           ++LIF++  L   +  K +    + PP P G PIIG+LH LG+L H+ L  LS+ YGP+M
Sbjct: 7   LSLIFVICILVAVFNHKNRRNYQRTPPSPPGCPIIGNLHQLGELPHQSLWKLSKKYGPVM 66

Query: 69  HIQLGLLPAIIVSSARATELFLKTHDLHFASRPLTITSNHISYGRKGIVFAQYGPYWRNI 128
            ++LG +P +IVSS+   +  LK HDLH  SRP    +  +SY    I F+ Y  YW+ +
Sbjct: 67  LLKLGRVPTVIVSSSETAKQALKIHDLHCCSRPGFAGARELSYNYLDIAFSPYDDYWKEV 126

Query: 129 RKMCTLELLSSLKINSFSSMRKQEVGSLIKGLEDAATDGVAVDLTSKISSVIGDMICVMV 188
           RK+   EL SS +++S   ++ +EV  LI  + ++A     ++L   + ++   ++C   
Sbjct: 127 RKLAVQELFSSKQVHSIQPIKDEEVKKLIDSISESAAQKTPINLNKTLLALTVSVVCRTA 186

Query: 189 LGRKYEDNELGEKGFKGLIREATQLAAAPNLGDFIPLIARFD--VQGFGGRAKAVGKIFD 248
               +E   L  + F  ++REA ++  + +  DFIP + R    + G  GR +   +  D
Sbjct: 187 FSVNFEGTVLNSERFNNIVREALEMLGSFSASDFIPYVGRIIDLLTGLQGRRERSMRDLD 246

Query: 249 GFLESIVEEHVVFEKDNKDKDFVDVLLDLMGSREY----QIDRSNIKAIILDFVIAAVDS 308
            F E + + H   +K+   +DFVD+LL L          ++ R++IKAI++D ++A +D+
Sbjct: 247 AFYEQMFDLHKQ-KKEEGSEDFVDLLLRLEKEEAVLGNDKLTRNHIKAILMDVLLAGMDT 306

Query: 309 TTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVMKESLRMHPP 368
           +  T+ W ++EL K+P +MKK+Q E+   +     +   D   L+YL+MV+KE+ R+HP 
Sbjct: 307 SAITMTWAMAELAKNPRVMKKVQSEIRSQIKNKERISFDDTDKLEYLKMVIKETWRLHPT 366

Query: 369 VP-LIPRECIQDCNINGYHIPKKSRIVINAWAIGRDPNTWVDPHKFDPERFLESEVDVRG 428
            P LIPRE + +  INGY IP K+R+ +N WAIGRDP+TW DP  F PERF ++ +D +G
Sbjct: 367 TPLLIPREAMSEFEINGYTIPVKTRLHVNVWAIGRDPDTWKDPEVFLPERFTDNNIDAKG 426

Query: 429 RDFELIPFGSGRRGCVGIQLALVVVRLVVAQLLHCFDLKLPDGMSPLELDMTEILGLTCP 488
           + FEL+PFG GRR C  + +   +V   +A LL+ FD KLP+GM   ++DM E  GLT  
Sbjct: 427 QHFELLPFGGGRRMCPAVYMGTTMVEFGLANLLYHFDWKLPEGMKVDDIDMEEAPGLTVN 486

Query: 489 RAQNLRVIPIFRLCP 497
           +   L ++P   L P
Sbjct: 487 KKNELILVPTKFLDP 500

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0A068Q5V61.7e-16258.84Cytochrome P450 71AU50 OS=Prunus mume OX=102107 GN=CYP71AU50 PE=2 SV=1[more]
H2DH182.2e-13349.90Cytochrome P450 CYP736A12 OS=Panax ginseng OX=4054 PE=2 SV=1[more]
P244653.3e-10542.27Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2[more]
A0A068Q7211.3e-10440.73Cytochrome P450 71AP13 OS=Prunus mume OX=102107 GN=CYP71AP13 PE=2 SV=1[more]
A0A068Q6L22.4e-10340.16Cytochrome P450 736A117 OS=Prunus mume OX=102107 GN=CYP736A117 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3CM911.0e-287100.00Cytochrome P450 CYP736A12-like protein OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3B2K51.0e-287100.00cytochrome P450 CYP736A12-like OS=Cucumis melo OX=3656 GN=LOC103485291 PE=3 SV=1[more]
A0A5A7T3X61.7e-28599.40Cytochrome P450 CYP736A12-like protein OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A6J1BYU01.0e-18163.38cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005927 PE... [more]
A0A6J1BTE36.5e-18164.24cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005393 PE... [more]
Match NameE-valueIdentityDescription
XP_008441066.10.0100.00PREDICTED: cytochrome P450 CYP736A12-like [Cucumis melo] >TYK12655.1 cytochrome ... [more]
KAA0036261.10.099.40cytochrome P450 CYP736A12-like protein [Cucumis melo var. makuwa][more]
XP_023543453.11.29e-25372.62cytochrome P450 CYP736A12-like [Cucurbita pepo subsp. pepo][more]
XP_022133318.15.33e-22863.38cytochrome P450 CYP736A12-like [Momordica charantia][more]
XP_022132564.18.75e-22764.24cytochrome P450 CYP736A12-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT5G07990.11.6e-10240.40Cytochrome P450 superfamily protein [more]
AT4G36220.13.9e-10140.29ferulic acid 5-hydroxylase 1 [more]
AT1G13080.15.2e-9837.33cytochrome P450, family 71, subfamily B, polypeptide 2 [more]
AT3G26310.13.8e-9637.20cytochrome P450, family 71, subfamily B, polypeptide 35 [more]
AT3G26300.16.4e-9636.97cytochrome P450, family 71, subfamily B, polypeptide 34 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 321..341
NoneNo IPR availablePANTHERPTHR47943:SF2CYTOCHROME P450 FAMILY 71 PROTEINcoord: 1..494
NoneNo IPR availablePANTHERPTHR47943CYTOCHROME P450 93A3-LIKEcoord: 1..494
IPR001128Cytochrome P450PRINTSPR00385P450coord: 298..315
score: 29.63
coord: 427..436
score: 57.4
coord: 436..447
score: 34.65
coord: 351..362
score: 37.98
IPR001128Cytochrome P450PFAMPF00067p450coord: 34..478
e-value: 6.3E-103
score: 345.0
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 179..197
score: 18.51
coord: 350..368
score: 27.73
coord: 390..414
score: 40.81
coord: 287..304
score: 24.66
coord: 307..333
score: 37.39
coord: 436..459
score: 33.45
coord: 61..80
score: 30.12
coord: 426..436
score: 53.79
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 26..491
e-value: 5.8E-128
score: 429.4
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 34..493
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 429..438

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0010812.1IVF0010812.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen