IVF0008225 (gene) Melon (IVF77) v1

Overview
NameIVF0008225
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionDiacylglycerol O-acyltransferase
Locationchr01: 2126946 .. 2129757 (+)
RNA-Seq ExpressionIVF0008225
SyntenyIVF0008225
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTCTTCTCTCTTACAACAACCACTAAGTCCTATTGCTGAATACTTAAGCTCCCCCATGCTCTCTCTTTCGGTTCTAGCTATCTTGGAATTCCAAGTCCCAATTCACACCTTGGATCTTGCTCCCAATCTTCGGAACCTCTTCCTCCCCATCAACCCTCGGTTTTCCTCCATATTGGTAATATGAAGAAAAGATTGTACCTATCAACTTAAACTTTTAAGTTTCGTTCGCATATAGTAGTTTAATATTATATTGTACCATAGCAAGAGACAAAACTTGTATTGAAAATCATTGTAGCATCAATTTTCTTTTGTCTCTATCAACATGAGCTTAGTAGTGGTTTAATTTATTTGTGTTCGAAACAAGATGTCATGTATTCAAACTCATATAATGACATTTTTTCGGAATTAGTGTTGTTGAAGACATATATGTTAACTTGAAGTTGATCATAAGAATTTAAAATTGTATGTTAAAGGCAAGATATCTTGTATTCAAATTCTTCTTTTTGCAAAATTGTATAATCCATGTCGTTTCATTTTAGATTTTGTTTTTTTTCCGATTAATATTAATATTGATAGTCATTTGAACATATTGAAATATTGTGTAAAATAATATATTTAGCTTAATAGCTAGACATACGTCATTGGAGCATATAAACCATTCATATATGTATTTAAAATTCTCATTCAAATGTAAAATTTCTATAGTCACAGCTTAATATCTTCCCTTCTCAACTTGTATCGAATGACAGGTTACAAATGAGCAAGGAGAGAAGAAGTGGAAAAGGGTAAACGTAGAAGTGGAAGAGCATGTGAAAGATCCAATCTTTCCACCTGGTTTAACTATAGAATCATACAAAGCTCATTTTGATGAGTACTTAACGAAGATATCCATGGAAGAATTTCGACCCGACAAACCACTATGGGAAACTCATTTGTTCAAGTATCCAACATCCCCAACCGTCGCCGGAACCATGATTCTAAAGCTCCACCACTCCCTCGGCGATGGCTATTCTCTCATGGGTGTCGTTCTTTCTTGTCTCCACACAGCCTCAGACCCTAATGTTCCTCTCACTTTTCCTTCCCGACGTCAACGACGATTCAACAACGGTGTTGTGAAGTCTTTGACTGGAATTGTGTCCGCTGTTTGTAGAACGGCGTCGGATTTGGGGTGGAGCGTTTTTAAAGGAATAGATGAGAAAACTCCTATAAGGTCAGGACGTGAAGGGCTACAGTTCATGCCGATGAATACTTTTACCGTGTCGTTTTCGCTCAATGACATAAAGAAAATCAAGACGAAGCTCCAAGTGGTGAGTTTTGTAATGAAAAACACATACATATTGAAGCTGCGAAAAAAAAAATCATGTCTCCATGTCTGCAGCATACACCAACCTCAACTGAATGTCGAGAAAATTCATAAAATGATATCATCATGAATTAATTGAATTCATCCTTCTCAGTCACGTATTAAACTCATAACCCTTTTAACCACTAGGGTTAACAATTTATTTTAGTCTCTCAGTTTTTGTAATACCCAATTTAGTCATTAACTCATTTTTTAAGAAAAAAAAAGTTCCATTGTTTGGTCATTACCATTACTATGCATTTTAACTAATGAGTGTTTTAATTTTTTTTTTCTTCACTGAAAAATGCAGACAGTAAATGATGTGATAACAGGGATAATCTTCATGGGACTAAGGTTGTATATGGAAGAAATAGAGAAAAATTCCGGTGAAGTCGCAGCAACGGCATTGACAATAGTGAACACGAGAGTGATTGGAAACTATCGGTCGGCGACAGAGATGGCAAAACCAGAGAGCAAAGGACTATGGGGAAACCAATTATCTTACTTGGAAATTATGATCCCAAAAATGACCAACGTCACTAACCCACTAGAGTTTGTCATGGCTGCTCATCGCTCCATCAACAACAAACGAACCTCCTTCACTATTCATGTTGTGGCTCATCTTTTGAATCTCTTGAGAAAACTTCGAGGTCATCAGGTAAAATAATTATTACTATATAATATATAATATTACTTTAACCATTGTTTTCGATTTGACCTATCCCATTCGATTTAGTTTACTGTGGTTCAAAAGCTCGAACATCGTCATTAGCATTGTTTAACAACAAAAAAAAACTTGGGTGCATCCTGTAATTCTATTATATAACTGCTAAAAAAATATGTAGGTGAGTCCATGTTTTTAACACGATATTAGAGGATAAAAGGGTATGTAGAGATTCAAATTTGAAACTAAGTAAAGTTCAATAATTAAGACTGAATGAAAACTTTTAAAATGGTAATTAAATAGATCGAATGTGATTGAACAATATAGTTTCTTAATTTTTTTAAATGATTTAATGTAGTTTGTAGTTAATTTAGATAATTTATGTAACCCGACACTGTATATATATAATTTGGCAGGAAGTAGCAAAGTTCCTCCACAATTTGTTGAAGAACACCACCACAGTAATCTCAAATGTGATTGGTCCACTTCAGCAAATGGCTTTAGATAATCATCCCATTAGCGGCTTATACTTCACCATTGTGGGCACACCTCAGGTTTTTATTTCATTGTTTATTTGGGTATTTTTTTGTATATAATATATTTTTAATCTCAACTCATATATATGTGTGTGTGTGTGTGTGTGTTTCTTTTGCAGAGTGTTACGGTAACGATGTTGAGTTATATGGGAAACTGAGAGTTGCATTCAGAACAGAAAAGGACTTCATTGATGCCCACAAGTTAAATTGTTGTATGGAAGATGCATTTGAGAAGATATTTGAAGCAGCAAATGATATTCCTTGA

mRNA sequence

ATGGATTCTTCTCTCTTACAACAACCACTAAGTCCTATTGCTGAATACTTAAGCTCCCCCATGCTCTCTCTTTCGGTTCTAGCTATCTTGGAATTCCAAGTCCCAATTCACACCTTGGATCTTGCTCCCAATCTTCGGAACCTCTTCCTCCCCATCAACCCTCGGTTTTCCTCCATATTGGTTACAAATGAGCAAGGAGAGAAGAAGTGGAAAAGGGTAAACGTAGAAGTGGAAGAGCATGTGAAAGATCCAATCTTTCCACCTGGTTTAACTATAGAATCATACAAAGCTCATTTTGATGAGTACTTAACGAAGATATCCATGGAAGAATTTCGACCCGACAAACCACTATGGGAAACTCATTTGTTCAAGTATCCAACATCCCCAACCGTCGCCGGAACCATGATTCTAAAGCTCCACCACTCCCTCGGCGATGGCTATTCTCTCATGGGTGTCGTTCTTTCTTGTCTCCACACAGCCTCAGACCCTAATGTTCCTCTCACTTTTCCTTCCCGACGTCAACGACGATTCAACAACGGTGTTGTGAAGTCTTTGACTGGAATTGTGTCCGCTGTTTGTAGAACGGCGTCGGATTTGGGGTGGAGCGTTTTTAAAGGAATAGATGAGAAAACTCCTATAAGGTCAGGACGTGAAGGGCTACAGTTCATGCCGATGAATACTTTTACCGTGTCGTTTTCGCTCAATGACATAAAGAAAATCAAGACGAAGCTCCAAGTGACAGTAAATGATGTGATAACAGGGATAATCTTCATGGGACTAAGGTTGTATATGGAAGAAATAGAGAAAAATTCCGGTGAAGTCGCAGCAACGGCATTGACAATAGTGAACACGAGAGTGATTGGAAACTATCGGTCGGCGACAGAGATGGCAAAACCAGAGAGCAAAGGACTATGGGGAAACCAATTATCTTACTTGGAAATTATGATCCCAAAAATGACCAACGTCACTAACCCACTAGAGTTTGTCATGGCTGCTCATCGCTCCATCAACAACAAACGAACCTCCTTCACTATTCATGTTGTGGCTCATCTTTTGAATCTCTTGAGAAAACTTCGAGGTCATCAGGAAGTAGCAAAGTTCCTCCACAATTTGTTGAAGAACACCACCACAGTAATCTCAAATGTGATTGGTCCACTTCAGCAAATGGCTTTAGATAATCATCCCATTAGCGGCTTATACTTCACCATTGTGGGCACACCTCAGTGTTACGGTAACGATGTTGAGTTATATGGGAAACTGAGAGTTGCATTCAGAACAGAAAAGGACTTCATTGATGCCCACAAGTTAAATTGTTGTATGGAAGATGCATTTGAGAAGATATTTGAAGCAGCAAATGATATTCCTTGA

Coding sequence (CDS)

ATGGATTCTTCTCTCTTACAACAACCACTAAGTCCTATTGCTGAATACTTAAGCTCCCCCATGCTCTCTCTTTCGGTTCTAGCTATCTTGGAATTCCAAGTCCCAATTCACACCTTGGATCTTGCTCCCAATCTTCGGAACCTCTTCCTCCCCATCAACCCTCGGTTTTCCTCCATATTGGTTACAAATGAGCAAGGAGAGAAGAAGTGGAAAAGGGTAAACGTAGAAGTGGAAGAGCATGTGAAAGATCCAATCTTTCCACCTGGTTTAACTATAGAATCATACAAAGCTCATTTTGATGAGTACTTAACGAAGATATCCATGGAAGAATTTCGACCCGACAAACCACTATGGGAAACTCATTTGTTCAAGTATCCAACATCCCCAACCGTCGCCGGAACCATGATTCTAAAGCTCCACCACTCCCTCGGCGATGGCTATTCTCTCATGGGTGTCGTTCTTTCTTGTCTCCACACAGCCTCAGACCCTAATGTTCCTCTCACTTTTCCTTCCCGACGTCAACGACGATTCAACAACGGTGTTGTGAAGTCTTTGACTGGAATTGTGTCCGCTGTTTGTAGAACGGCGTCGGATTTGGGGTGGAGCGTTTTTAAAGGAATAGATGAGAAAACTCCTATAAGGTCAGGACGTGAAGGGCTACAGTTCATGCCGATGAATACTTTTACCGTGTCGTTTTCGCTCAATGACATAAAGAAAATCAAGACGAAGCTCCAAGTGACAGTAAATGATGTGATAACAGGGATAATCTTCATGGGACTAAGGTTGTATATGGAAGAAATAGAGAAAAATTCCGGTGAAGTCGCAGCAACGGCATTGACAATAGTGAACACGAGAGTGATTGGAAACTATCGGTCGGCGACAGAGATGGCAAAACCAGAGAGCAAAGGACTATGGGGAAACCAATTATCTTACTTGGAAATTATGATCCCAAAAATGACCAACGTCACTAACCCACTAGAGTTTGTCATGGCTGCTCATCGCTCCATCAACAACAAACGAACCTCCTTCACTATTCATGTTGTGGCTCATCTTTTGAATCTCTTGAGAAAACTTCGAGGTCATCAGGAAGTAGCAAAGTTCCTCCACAATTTGTTGAAGAACACCACCACAGTAATCTCAAATGTGATTGGTCCACTTCAGCAAATGGCTTTAGATAATCATCCCATTAGCGGCTTATACTTCACCATTGTGGGCACACCTCAGTGTTACGGTAACGATGTTGAGTTATATGGGAAACTGAGAGTTGCATTCAGAACAGAAAAGGACTTCATTGATGCCCACAAGTTAAATTGTTGTATGGAAGATGCATTTGAGAAGATATTTGAAGCAGCAAATGATATTCCTTGA

Protein sequence

MDSSLLQQPLSPIAEYLSSPMLSLSVLAILEFQVPIHTLDLAPNLRNLFLPINPRFSSILVTNEQGEKKWKRVNVEVEEHVKDPIFPPGLTIESYKAHFDEYLTKISMEEFRPDKPLWETHLFKYPTSPTVAGTMILKLHHSLGDGYSLMGVVLSCLHTASDPNVPLTFPSRRQRRFNNGVVKSLTGIVSAVCRTASDLGWSVFKGIDEKTPIRSGREGLQFMPMNTFTVSFSLNDIKKIKTKLQVTVNDVITGIIFMGLRLYMEEIEKNSGEVAATALTIVNTRVIGNYRSATEMAKPESKGLWGNQLSYLEIMIPKMTNVTNPLEFVMAAHRSINNKRTSFTIHVVAHLLNLLRKLRGHQEVAKFLHNLLKNTTTVISNVIGPLQQMALDNHPISGLYFTIVGTPQCYGNDVELYGKLRVAFRTEKDFIDAHKLNCCMEDAFEKIFEAANDIP
Homology
BLAST of IVF0008225 vs. ExPASy Swiss-Prot
Match: Q9M3B3 (Wax ester synthase/diacylglycerol acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=WSD4 PE=2 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 1.8e-41
Identity = 131/474 (27.64%), Postives = 225/474 (47.47%), Query Frame = 0

Query: 7   QQPLSPIAEYLSSPMLSLSVLAILEFQVPIHTLDLAPNLRNLFLPINPRFSSILVTNEQG 66
           +QPLSP A    +P  + ++++++ F+  +         +  F+  +PRFSS LVT+E G
Sbjct: 17  EQPLSPAARLFHAPEFNCNIISVIGFKSKLDPCVFIRGFKESFIR-HPRFSSKLVTDENG 76

Query: 67  E-KKWKRVNVEVEEHVKDPIFPPGLTIESYKAHFDEYLTKISMEEFRPDKPLWETHLFKY 126
           + ++W R NV VE+H   P   P   IE+  A  ++Y++ +        +PLWE HL   
Sbjct: 77  QNQRWVRTNVVVEDHFIVPKIKP-QNIENSNAFLEDYVSDLMKIPLDTSRPLWELHLLDL 136

Query: 127 PTSPTVAGTMILKLHHSLGDGYSLMGVVLSCLHTASD----PNVP-----------LTFP 186
            TS       +LK+HHS+GDG S+M +VL+C+   S+    P++P           LT  
Sbjct: 137 KTS-DAENVAVLKIHHSVGDGMSIMSLVLACMRKTSNPDELPSLPYQYRSSSRSSLLTTG 196

Query: 187 SRRQRRFNNGVVKSLTGIV---SAVCRTASDLGWSVFKGIDEKTPIRSGREGLQFMPMNT 246
           SR   R    V    T ++   + VC     +  ++F   D +TPI+      +   +  
Sbjct: 197 SRSDSRLLWLVKVIWTAVILGLNTVCDALEFIVTTLFVK-DTETPIKGDFLSTKSKQLRL 256

Query: 247 FTVSFSLNDIKKIKTKLQVTVNDVITGIIFMGL-----RLYMEEIEKNSGE------VAA 306
              + SL+DIK  K  + +T+NDV+ G+   GL     R Y EE  KN  +      +  
Sbjct: 257 VHRTVSLDDIKLTKNAMNMTINDVVLGVTQAGLSRYLARRYGEEETKNRKQKKLPKRIRL 316

Query: 307 TALTIVNTRVIGNYRSATEMAKPESKGLWGNQLSYLEIMIP-KMTNVTNPLEFVMAAHRS 366
            +  +VN R     +   +M +  SK  WGN   Y  ++ P  +    +PLE +  A ++
Sbjct: 317 RSALLVNLRPTTGIQDLADMMEKGSKCRWGNWFGY--VVFPFSIALRDDPLEHLEIAQKT 376

Query: 367 INNKRTSFTIHVVAHLLNLLRKLRGHQEVAKFLHNLLKNTTTVISNVIGPLQQMALDNHP 426
           I+ K+ S+   +      ++ KL G +  A  ++ ++ NTT   SN++GP+++++   HP
Sbjct: 377 ISRKKNSYGAMLTYIFCRIIVKLLGIKVAASIINRMVSNTTMTFSNMVGPVEEVSFYGHP 436

Query: 427 ISGLYFTIVGTPQCYGNDVELY-GKLRVAFRTEKDFI-DAHKLNCCMEDAFEKI 448
           I+    +  G P       + Y  K+ +    +   I D H+L    E++   I
Sbjct: 437 ITYFASSAYGHPHALTIHCQSYMNKMTITLIVDPTVISDPHRLCDDWEESLRSI 484

BLAST of IVF0008225 vs. ExPASy Swiss-Prot
Match: Q5KS41 (Wax ester synthase/diacylglycerol acyltransferase 11 OS=Arabidopsis thaliana OX=3702 GN=WSD11 PE=1 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 8.9e-41
Identity = 130/455 (28.57%), Postives = 218/455 (47.91%), Query Frame = 0

Query: 7   QQPLSPIAEYLSSPMLSLSVLAILEFQVPIHTLDLAPNLRNLFLPINPRFSSILVTNEQG 66
           ++PLSP +   +SP  + +++  +  +V   T  +   L +  L  +PRFSSIL  N   
Sbjct: 14  EEPLSPCSRLFNSPDFNCAIIVTMGSKVKGDTPAIIHGLEHT-LVNHPRFSSILEMNNGK 73

Query: 67  EKKWKRVNVEVEEHVKDPIFPPGLTIESYKAHFDEYLTKISMEEFRPDKPLWETHLFKYP 126
           + +W R  V+VEEHV  P   P   IE+   + ++Y++K++       KPLWE HL    
Sbjct: 74  KPRWVRTKVKVEEHVIVPDVDP--DIENPDQYLEDYISKLTTIPMDLSKPLWEMHLLGVK 133

Query: 127 TSPTVAGTMILKLHHSLGDGYSLMGVVLSCLHTASDPNVPLTFPSRRQR---RFNNG--- 186
           TS       ILK+HHSLGDG SLM ++L+C    SDP    T    ++R     N+G   
Sbjct: 134 TS-NAESYAILKIHHSLGDGMSLMSLLLACTRKTSDPEALPTVAVHKKRFGPSCNSGFFN 193

Query: 187 ----VVKSLTGIVSAVCRTASDL---GWSVFKGIDEKTPIRSGREGLQFMPMNTFTVSFS 246
               +   L  I+  +  T  D+     ++F   D +TP+ + + G + +P        S
Sbjct: 194 KIWWLFVGLWFILRLLFNTFVDILMFALTIFVLRDTETPLLA-KPGSELIPKRFVHRIIS 253

Query: 247 LNDIKKIKTKLQVTVNDVITGIIFMGL-----RLYMEEIEKNSGE----VAATALTIVNT 306
            +D+K +K  +++TVNDV+ G+   GL     R Y +E    S E    +   +  ++N 
Sbjct: 254 FDDVKLVKNAMKMTVNDVLLGVTQAGLSRYLSRKYDQEATPKSKESMRRIRLRSAIMINL 313

Query: 307 RVIGNYRSATEMAKPESKGLWGNQLSYLEIMIPKMTNV-TNPLEFVMAAHRSINNKRTSF 366
           R      +  +M   +SK  WGN   Y  I++P    + T+PLE+V  A  +I+ K+ S 
Sbjct: 314 RPNAGIEALADMMAKKSKCRWGNLFGY--ILLPFSVGLETDPLEYVRQAKATIDRKKHSL 373

Query: 367 TIHVVAHLLNLLRKLRGHQEVAKFLHNLLKNTTTVISNVIGPLQQMALDNHPISGLYFTI 426
                     L+ K+ G +     +  ++ +TT   SNV+GP +++    HP++ +   +
Sbjct: 374 EAVFSMAFFKLILKVLGLKASVVLVRKVIHSTTLSFSNVVGPKEEITFHGHPLNYISPCV 433

Query: 427 VGTPQCYGNDVELY-GKLRVAFRTEKDFI-DAHKL 437
            G P       + Y  K+ ++   +   I D HK+
Sbjct: 434 FGHPHALTLHFQTYANKVIISVTADPTVIPDPHKM 461

BLAST of IVF0008225 vs. ExPASy Swiss-Prot
Match: Q9M3B2 (Wax ester synthase/diacylglycerol acyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=WSD5 PE=2 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 3.8e-39
Identity = 128/476 (26.89%), Postives = 221/476 (46.43%), Query Frame = 0

Query: 7   QQPLSPIAEYLSSPMLSLSVLAILEFQVPIHTLDLAPNLRNLFLPINPRFSSILV---TN 66
           +QPLSP A    +P  +  +++++  +  I    +   ++   +  +PRFSS LV    N
Sbjct: 25  EQPLSPAARLFHAPEFNCYIISVVGLKNKIEPDMIIEGIKQTLMR-HPRFSSKLVNNCNN 84

Query: 67  EQGEKKWKRVNVEVEEHVKDP-IFPPGLTIESYKAHFDEYLTKISMEEFRPDKPLWETHL 126
            + E+KW R NV VE+HV  P I    +   +     + Y++ ++       KPLWE HL
Sbjct: 85  NRQEQKWVRTNVVVEDHVIIPKIQTQHIENANADVFLESYVSDLTTIPLDTSKPLWEVHL 144

Query: 127 FKYPTSPTVAGTMILKLHHSLGDGYSLMGVVLSCLHTASDPNVPLTFPSRRQRRFNNGVV 186
               TS       +L++HHSLGDG S+M +VL+C    S+PN   + P + +    +  +
Sbjct: 145 LDLKTS-DAENVAVLRIHHSLGDGMSMMSLVLACTRKTSNPNELPSLPYQNRPSSGSSSL 204

Query: 187 KSLTG------------------IVSAVCRTASDLGWSVFKGIDEKTPIRSGREGLQFMP 246
           K+ +                   +++ VC     +  ++F   D +TPI+   +  +   
Sbjct: 205 KTSSRCYSRFFWLVMVLWSAALLVLNTVCDALEFIATALFLK-DTETPIKGDFKLSKGKR 264

Query: 247 MNTFTVSFSLNDIKKIKTKLQVTVNDVITGIIFMGLRLYM-------EEIEKNSGE---- 306
           M     + SL+DIK IK  +++TVNDV+ G+   GL  Y+       EE ++NS      
Sbjct: 265 MCMVHRTVSLDDIKLIKNAMKMTVNDVVLGVSQAGLSQYLKRRYGEQEESKRNSSNIPKG 324

Query: 307 VAATALTIVNTRVIGNYRSATEMAKPESKGLWGNQLSYLEIMIPKMTNVTNPLEFVMAAH 366
           +   A  +VN R     +   +M    SK  WGN + Y+ I    +    +PL+ +  A 
Sbjct: 325 IRLRAALLVNLRPTTGIQDLADMMTKGSKCRWGNWIGYI-IFPFSIALCDDPLKHLRRAK 384

Query: 367 RSINNKRTSFTIHVVAHLLNLLRKLRGHQEVAKFLHNLLKNTTTVISNVIGPLQQMALDN 426
            +I+ K+ S    +   +  +L    G Q  A  L+  L NTT   SN++GP+++++   
Sbjct: 385 STIDRKKNSLEAVLTFVVGKILLNTLGVQRAANVLNRALSNTTMSFSNLVGPVEEISFYG 444

Query: 427 HPISGLYFTIVGTPQCYGNDVELY-GKLRVAFRTEKDFI-DAHKLNCCMEDAFEKI 448
           H ++ +  ++ G P       + Y  KL ++   +   I D HKL    E++   I
Sbjct: 445 HTVTYIAPSVYGHPHALTMHFQSYMNKLTISLTVDPTVISDPHKLCDDWEESLRSI 496

BLAST of IVF0008225 vs. ExPASy Swiss-Prot
Match: Q94CK0 (Wax ester synthase/diacylglycerol acyltransferase 7 OS=Arabidopsis thaliana OX=3702 GN=WSD7 PE=1 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 6.4e-39
Identity = 129/477 (27.04%), Postives = 228/477 (47.80%), Query Frame = 0

Query: 7   QQPLSPIAEYLSSPMLSLSVLAILEFQVPIHTLDLAPNLRNLFLPINPRFSSILVTNEQG 66
           ++P+SP+A    SP + L  + I+ F+  I+  D+  +     +  +PRFSSIL  N   
Sbjct: 6   EEPVSPMARVFQSPGIDLCAVTIMGFKTKINP-DVVLDALKQNVSKHPRFSSILSDN--- 65

Query: 67  EKKWKRVNVEVEEHVKDPIFPPGLTIESYKAHFDEYLTKISMEEFRPDKPLWETHLFKYP 126
             KW    V VE+HV  P        E  ++  D+Y+++++M      +PLW+ H+    
Sbjct: 66  GAKWIETEVNVEDHVIVPYIDAEEIGEGGQSFIDDYMSRLTMIPLDRSRPLWDIHILNVK 125

Query: 127 TSPTVAGTMILKLHHSLGDGYSLMGVVLSCLHTASDPNV-PLTFPSRRQRRFNNGVVKSL 186
           TS   A    ++ HHSLGDG SL+ ++L+C H  SDP++     PS ++R   +  +K+ 
Sbjct: 126 TSEAEA-VGFIRSHHSLGDGMSLISLMLACTHKTSDPDMFSNAIPSMKRRATMSHSLKTK 185

Query: 187 TGIVSAVCRTASD--LGWSVFKGI-----------DEKTPIRSGREGLQFMPMNTFTVSF 246
              + ++    S   L W+    +           D KTP+++G + ++  P   +    
Sbjct: 186 GWFLRSIFTIGSTMRLLWNTTIDMLLLLATVLFLKDTKTPLKAGAD-VRSNPKRFYHRII 245

Query: 247 SLNDIKKIKTKLQVTVNDVITGIIFMGLRLYMEEI--EKNSGEVAATA------------ 306
           SL+DIK IK  + +T+NDV+ GI    L  Y+      K   + A T+            
Sbjct: 246 SLDDIKLIKNAMNMTINDVLFGITQASLSHYLNRQYGTKKEEDGALTSYRNNLPDGIRFR 305

Query: 307 -LTIVNTRVIGNYRSATEMAKPESKGLWGNQLSYLEIMIPKMTNV-TNPLEFVMAAHRSI 366
               VN R    ++   +M   +SK  WGN  S+  I +P    + T+PL ++  +   +
Sbjct: 306 VACTVNLRSDIGFKPLADMMVKDSKCRWGNYFSF--IFLPFTIGLQTDPLVYLKMSKSMM 365

Query: 367 NNKRTSFTIHVVAHLLNLLRKLRGHQEVAKFLHNLLKNTTTVISNVIGPLQQMALDNHPI 426
             K+ S+   +V  ++ ++ K+ G +  A+     ++NTTT +SNVIGP+++++   HP+
Sbjct: 366 ARKKHSYHAALVYFIIKIVLKVFGAKAAAELFDRPVRNTTTCVSNVIGPMEEISFRGHPV 425

Query: 427 SGLYFTIVGTPQCYGNDVELY-------GKLRVAFRTEKDFI-DAHKLNCCMEDAFE 446
           S +       P  YG+   L         K+ ++   +   I D HK+   ME++ +
Sbjct: 426 SYI------APSSYGHSHALLIHLMSYADKMIISLAYDPTVISDPHKICDDMEESLK 468

BLAST of IVF0008225 vs. ExPASy Swiss-Prot
Match: Q9FK89 (Wax ester synthase/diacylglycerol acyltransferase 9 OS=Arabidopsis thaliana OX=3702 GN=WSD9 PE=2 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 8.4e-39
Identity = 136/474 (28.69%), Postives = 224/474 (47.26%), Query Frame = 0

Query: 7   QQPLSPIAEYLSSPMLSLSVLAILEFQVPIHTLDLAPNLR-NLFLPINPRFSSILVTNEQ 66
           ++PLSP+A     P +   ++     +  I+   +  +L+ N+F   +PRF S+L   + 
Sbjct: 10  EEPLSPMARAFQEPSIDCGIVIKFGCKTKINPDVIVDSLKLNVFK--HPRFCSLL---DD 69

Query: 67  GEKKWKRVN-VEVEEHVKDPIFPPGLTIESYKAHFDEYLTKISMEEFRPDKPLWETHLFK 126
              KW R + V VEEHV  P   P LT E  +   ++Y++ I+M      KPLWE H+  
Sbjct: 70  DGTKWLRTDVVNVEEHVFVPDIDPKLTEEDVEWFVEDYISSITMIPLDRTKPLWEVHILN 129

Query: 127 YPTSPTVAGTMILKLHHSLGDGYSLMGVVLSCLHTASDPNVPLTFPSRRQRR-FNNGVVK 186
             TS   A   +++ HH+LGDG S++ ++L+     S+P    T P  + R  +N+    
Sbjct: 130 AKTSDAEA-ICVIRCHHALGDGVSILSLILASTRKTSEPEAFSTLPVPKCRESYNHRRGF 189

Query: 187 SLTGIVSAVCRTASDLGW-----------SVFKGIDEKTPIRSGREGLQFMPMNTFTVSF 246
           S   +V  VC T   L W           ++F   D  TP++ G+ G     +  F+   
Sbjct: 190 SFFRLVLVVCSTVR-LIWNTLVDSFLCMATIFFLKDTDTPLK-GKPG----AIKKFSHRI 249

Query: 247 -SLNDIKKIKTKLQVTVNDVITGIIFMGLRLYMEEIEKNSGEVAATALT----------- 306
            SL+DIK IK  +++T+NDV+ G+    L  Y+ +    + E A T+LT           
Sbjct: 250 VSLDDIKLIKNAMEMTINDVLLGVTEAALTRYLHQSYDKTNEEAGTSLTPNRQDLLDRIR 309

Query: 307 -----IVNTRVIGNYRSATEMAKPESKGLWGNQLSYLEIMIP-KMTNVTNPLEFVMAAHR 366
                +VN R  G+   A  MAK  SK  WGN +S   I+ P  +   ++PL ++     
Sbjct: 310 LRSLIVVNLRPTGSQSIADMMAK-GSKCRWGNYISV--ILFPFTIALQSDPLVYLSNVKS 369

Query: 367 SINNKRTSFTIHVVAHLLNLLRKLRGHQEVAKFLHNLLKNTTTVISNVIGPLQQMALDNH 426
            I+ K+ S   +++      + K  G      F   ++ NTT  ISN+ GP ++++   H
Sbjct: 370 MIDRKKNSLITYIIYTFSEFVIKAFGINVAVAFQRKIMLNTTMCISNLPGPTEEVSFHGH 429

Query: 427 PISGLYFTIVGTPQCYGNDVELY-GKLRVAFRTEKDFIDAHKLNCCMEDAFEKI 448
           PI+    +I G PQ        Y  K+ ++   +   IDAHKL   +E++ + +
Sbjct: 430 PIAYFAPSIYGLPQALTIHYLSYANKMIISVAVDPMIIDAHKLCDELEESLKNM 468

BLAST of IVF0008225 vs. ExPASy TrEMBL
Match: A0A5A7SKE3 (Diacylglycerol O-acyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G003010 PE=4 SV=1)

HSP 1 Score: 884.4 bits (2284), Expect = 2.0e-253
Identity = 447/456 (98.03%), Postives = 448/456 (98.25%), Query Frame = 0

Query: 1   MDSSLLQQPLSPIAEYLSSPMLSLSVLAILEFQVPIHTLDLAPNLRNLFLPINPRFSSIL 60
           MDSSLLQQPLSPIAEYLSSPMLSLSVLAILEFQVPIHTLDLAPNLRNLFLPINPRFSSIL
Sbjct: 1   MDSSLLQQPLSPIAEYLSSPMLSLSVLAILEFQVPIHTLDLAPNLRNLFLPINPRFSSIL 60

Query: 61  VTNEQGEKKWKRVNVEVEEHVKDPIFPPGLTIESYKAHFDEYLTKISMEEFRPDKPLWET 120
           VTNEQGEKKWKRVNVEVEEHVKDPIFPPGLTIESYKAHFDEYLTKISMEEFRPDKPLWET
Sbjct: 61  VTNEQGEKKWKRVNVEVEEHVKDPIFPPGLTIESYKAHFDEYLTKISMEEFRPDKPLWET 120

Query: 121 HLFKYPTSPTVAGTMILKLHHSLGDGYSLMGVVLSCLHTASDPNVPLTFPSRRQRRFNNG 180
           HLFKYPTSPTVAGTMILKLHHSLGDGYSLMGVVLSCLHTASDPNVPLTFPSRRQRRFNNG
Sbjct: 121 HLFKYPTSPTVAGTMILKLHHSLGDGYSLMGVVLSCLHTASDPNVPLTFPSRRQRRFNNG 180

Query: 181 VVKSLTGIVSAVCRTASDLGWSVFKGIDEKTPIRSGREGLQFMPMNTFTVSFSLNDIKKI 240
           VVKSLTGIVSAVCRTASDLGWSVFKGIDEKTPIRSGREGLQFMPMNTFTVSFSLNDIKKI
Sbjct: 181 VVKSLTGIVSAVCRTASDLGWSVFKGIDEKTPIRSGREGLQFMPMNTFTVSFSLNDIKKI 240

Query: 241 KTKLQVTVNDVITGIIFMGLRLYMEEIEKNSGEVAATALTIVNTRVIGNYRSATEMAKPE 300
           KTKLQVTVNDVITGIIFMGLRLYMEEIEKNSGEVAATALTIVNTRVIGNYRSATEMAKPE
Sbjct: 241 KTKLQVTVNDVITGIIFMGLRLYMEEIEKNSGEVAATALTIVNTRVIGNYRSATEMAKPE 300

Query: 301 SKGLWGNQLSYLEIMIPKMTNVTNPLEFVMAAHRSINNKRTSFTIHVVAHLLNLLRKLRG 360
           SKGLWGNQLSYLEIMIPKMTNVTNPLEFVMAAHRSINNKRTSFTIHVVAHLLNLLRKLRG
Sbjct: 301 SKGLWGNQLSYLEIMIPKMTNVTNPLEFVMAAHRSINNKRTSFTIHVVAHLLNLLRKLRG 360

Query: 361 HQEVAKFLHNLLKNTTTVISNVIGPLQQMALDNHPISGLYFTIVGTPQCYGNDVELY-GK 420
           HQEVAKFLHNLLKNTTTVISNVIGPLQQMALDNHPISGLYFTIVGTPQ     +  Y GK
Sbjct: 361 HQEVAKFLHNLLKNTTTVISNVIGPLQQMALDNHPISGLYFTIVGTPQSVTVTMLSYMGK 420

Query: 421 LRVAFRTEKDFIDAHKLNCCMEDAFEKIFEAANDIP 456
           LRVAFRTEKDFIDAHKLNCCMEDAFEKIFEAANDIP
Sbjct: 421 LRVAFRTEKDFIDAHKLNCCMEDAFEKIFEAANDIP 456

BLAST of IVF0008225 vs. ExPASy TrEMBL
Match: A0A1S3C1V4 (Diacylglycerol O-acyltransferase OS=Cucumis melo OX=3656 GN=LOC103495688 PE=4 SV=1)

HSP 1 Score: 884.4 bits (2284), Expect = 2.0e-253
Identity = 447/456 (98.03%), Postives = 448/456 (98.25%), Query Frame = 0

Query: 1   MDSSLLQQPLSPIAEYLSSPMLSLSVLAILEFQVPIHTLDLAPNLRNLFLPINPRFSSIL 60
           MDSSLLQQPLSPIAEYLSSPMLSLSVLAILEFQVPIHTLDLAPNLRNLFLPINPRFSSIL
Sbjct: 1   MDSSLLQQPLSPIAEYLSSPMLSLSVLAILEFQVPIHTLDLAPNLRNLFLPINPRFSSIL 60

Query: 61  VTNEQGEKKWKRVNVEVEEHVKDPIFPPGLTIESYKAHFDEYLTKISMEEFRPDKPLWET 120
           VTNEQGEKKWKRVNVEVEEHVKDPIFPPGLTIESYKAHFDEYLTKISMEEFRPDKPLWET
Sbjct: 61  VTNEQGEKKWKRVNVEVEEHVKDPIFPPGLTIESYKAHFDEYLTKISMEEFRPDKPLWET 120

Query: 121 HLFKYPTSPTVAGTMILKLHHSLGDGYSLMGVVLSCLHTASDPNVPLTFPSRRQRRFNNG 180
           HLFKYPTSPTVAGTMILKLHHSLGDGYSLMGVVLSCLHTASDPNVPLTFPSRRQRRFNNG
Sbjct: 121 HLFKYPTSPTVAGTMILKLHHSLGDGYSLMGVVLSCLHTASDPNVPLTFPSRRQRRFNNG 180

Query: 181 VVKSLTGIVSAVCRTASDLGWSVFKGIDEKTPIRSGREGLQFMPMNTFTVSFSLNDIKKI 240
           VVKSLTGIVSAVCRTASDLGWSVFKGIDEKTPIRSGREGLQFMPMNTFTVSFSLNDIKKI
Sbjct: 181 VVKSLTGIVSAVCRTASDLGWSVFKGIDEKTPIRSGREGLQFMPMNTFTVSFSLNDIKKI 240

Query: 241 KTKLQVTVNDVITGIIFMGLRLYMEEIEKNSGEVAATALTIVNTRVIGNYRSATEMAKPE 300
           KTKLQVTVNDVITGIIFMGLRLYMEEIEKNSGEVAATALTIVNTRVIGNYRSATEMAKPE
Sbjct: 241 KTKLQVTVNDVITGIIFMGLRLYMEEIEKNSGEVAATALTIVNTRVIGNYRSATEMAKPE 300

Query: 301 SKGLWGNQLSYLEIMIPKMTNVTNPLEFVMAAHRSINNKRTSFTIHVVAHLLNLLRKLRG 360
           SKGLWGNQLSYLEIMIPKMTNVTNPLEFVMAAHRSINNKRTSFTIHVVAHLLNLLRKLRG
Sbjct: 301 SKGLWGNQLSYLEIMIPKMTNVTNPLEFVMAAHRSINNKRTSFTIHVVAHLLNLLRKLRG 360

Query: 361 HQEVAKFLHNLLKNTTTVISNVIGPLQQMALDNHPISGLYFTIVGTPQCYGNDVELY-GK 420
           HQEVAKFLHNLLKNTTTVISNVIGPLQQMALDNHPISGLYFTIVGTPQ     +  Y GK
Sbjct: 361 HQEVAKFLHNLLKNTTTVISNVIGPLQQMALDNHPISGLYFTIVGTPQSVTVTMLSYMGK 420

Query: 421 LRVAFRTEKDFIDAHKLNCCMEDAFEKIFEAANDIP 456
           LRVAFRTEKDFIDAHKLNCCMEDAFEKIFEAANDIP
Sbjct: 421 LRVAFRTEKDFIDAHKLNCCMEDAFEKIFEAANDIP 456

BLAST of IVF0008225 vs. ExPASy TrEMBL
Match: A0A438JK81 (Diacylglycerol O-acyltransferase OS=Vitis vinifera OX=29760 GN=WSD1_5 PE=4 SV=1)

HSP 1 Score: 453.4 bits (1165), Expect = 1.1e-123
Identity = 218/457 (47.70%), Postives = 318/457 (69.58%), Query Frame = 0

Query: 8   QPLSPIAEYLSSPMLSLSVLAILEFQVPIHTLDLAPNLRNLFLPINPRFSSILVTNEQGE 67
           QP+SP  +Y +S +L +S+++ILE  +PI  L     L+++FLPINPRFSS++V ++ G 
Sbjct: 13  QPVSPTGQYFNSSVLQISIMSILELDIPIDDLPTLSLLKDVFLPINPRFSSLMVEDKNGV 72

Query: 68  KKWKRVNVEVEEHVKDPIFPPGLTIESYKAHFDEYLTKISMEEFRPDKPLWETHLFKYPT 127
           K+WKRV V++++HV  P+FP GL+ ESY  +FD+YLTKI+M+EF   +PLWE H+ KYPT
Sbjct: 73  KQWKRVEVKLQDHVNVPVFPDGLSPESYDDYFDDYLTKIAMKEFPQSRPLWEIHIIKYPT 132

Query: 128 SPTVAGTMILKLHHSLGDGYSLMGVVLSCLHTASDPNVPLTFPS-RRQRRFNNG--VVKS 187
           S   AGT++ KLHH+LGDG+SLMG +LSCL  A +PN+P+TFPS +     + G  ++++
Sbjct: 133 S-NAAGTVVFKLHHALGDGFSLMGALLSCLQRADNPNLPITFPSLKASSNLDKGRSIIRA 192

Query: 188 LTGIVSAVCRTASDLGWSVFKGI---DEKTPIRSGREGLQFMPMNTFTVSFSLNDIKKIK 247
           +   +SA   T SD GWS+ K     D++TPIRSG  G++F P++  T+SFSL+ I+KIK
Sbjct: 193 VPRALSAAFNTVSDFGWSLLKSTVVEDDRTPIRSGDPGVEFRPLSIATISFSLDYIQKIK 252

Query: 248 TKLQVTVNDVITGIIFMGLRLYMEEIEKNSGEVAATALTIVNTRVIGNYRSATEMAKPES 307
            KL VT+NDV+TGIIF G RLYM+ +   S    +TAL ++NTRVI  Y+S  EM   +S
Sbjct: 253 AKLGVTINDVLTGIIFFGTRLYMQSMNHASRNANSTALVLLNTRVISGYKSLKEMTASDS 312

Query: 308 KGLWGNQLSYLEIMIPKMTNV--TNPLEFVMAAHRSINNKRTSFTIHVVAHLLNLLRKLR 367
              WGNQ ++L + +P++ +   T+PL+FV  A ++I  KR S  +H+   LL  LRK R
Sbjct: 313 SSQWGNQFAFLHVTLPELADAKFTSPLDFVAKAQQTIQRKRNSLAVHLTGRLLETLRKYR 372

Query: 368 GHQEVAKFLHNLLKNTTTVISNVIGPLQQMALDNHPISGLYFTIVGTPQCYGNDVELY-G 427
           G +  A+++H  LKN++  I+N+IGP +Q+ALDNHP  G+YF ++G+P+     +  Y G
Sbjct: 373 GPEVTARYIHGTLKNSSMAITNMIGPTEQVALDNHPCRGIYFMVLGSPESMTISIVSYMG 432

Query: 428 KLRVAFRTEKDFIDAHKLNCCMEDAFEKIFEAANDIP 456
           K+R+A  TEK FID  K N C+E+AF+++FEAA   P
Sbjct: 433 KVRIAVGTEKGFIDPRKFNACIENAFQRVFEAALGTP 468

BLAST of IVF0008225 vs. ExPASy TrEMBL
Match: F6H5E6 (Diacylglycerol O-acyltransferase OS=Vitis vinifera OX=29760 GN=VIT_12s0028g03480 PE=4 SV=1)

HSP 1 Score: 451.1 bits (1159), Expect = 5.7e-123
Identity = 219/457 (47.92%), Postives = 318/457 (69.58%), Query Frame = 0

Query: 8   QPLSPIAEYLSSPMLSLSVLAILEFQVPIHTLDLAPNLRNLFLPINPRFSSILVTNEQGE 67
           QP+SP  +Y +S +L +S+++ILE  +PI        L+++FLPINPRFSS++V +++G 
Sbjct: 13  QPVSPTGQYFNSSVLQVSIMSILESDIPIDDSPTLSLLKDVFLPINPRFSSLMVEDKKGV 72

Query: 68  KKWKRVNVEVEEHVKDPIFPPGLTIESYKAHFDEYLTKISMEEFRPDKPLWETHLFKYPT 127
           K WKRV V++E+HV  PIFP GL+ ESY  +FD+YLTKI+M+EF   +PLWE H+ KYPT
Sbjct: 73  KHWKRVEVKLEDHVNVPIFPDGLSPESYDDYFDDYLTKIAMKEFPQSRPLWEIHIIKYPT 132

Query: 128 SPTVAGTMILKLHHSLGDGYSLMGVVLSCLHTASDPNVPLTFPS-RRQRRFNNG--VVKS 187
           S   AGT++ KLHH+LGDG+SLMG +LSCL  A +PN+P+TFPS +     ++G  ++++
Sbjct: 133 S-NAAGTVVFKLHHALGDGFSLMGALLSCLQRADNPNLPITFPSFKVSSNLDSGRSIIRA 192

Query: 188 LTGIVSAVCRTASDLGWSVFKGI---DEKTPIRSGREGLQFMPMNTFTVSFSLNDIKKIK 247
           +   +SA   T SD GW + K     D++TPIRSG  G++F PM+  T+SFSL++I+KIK
Sbjct: 193 VPRALSAAFNTVSDFGWGLLKSTAVEDDRTPIRSGDPGVEFRPMSIATISFSLDNIQKIK 252

Query: 248 TKLQVTVNDVITGIIFMGLRLYMEEIEKNSGEVAATALTIVNTRVIGNYRSATEMAKPES 307
            KL VT+NDV+TGIIF G RLYM+ +   S    +TAL ++NTRVI  Y+S  EM   +S
Sbjct: 253 AKLGVTINDVLTGIIFFGTRLYMQSMNHASRNANSTALVLLNTRVISGYKSIKEMTASDS 312

Query: 308 KGLWGNQLSYLEIMIPKMTNV--TNPLEFVMAAHRSINNKRTSFTIHVVAHLLNLLRKLR 367
              WGNQ ++L + +P++ +   T+PL+FV  A ++I  KR S  +H+   LL  LRK R
Sbjct: 313 SSQWGNQFAFLHVTLPELADAKFTSPLDFVAKAQQTIQRKRNSLAVHLTGRLLETLRKYR 372

Query: 368 GHQEVAKFLHNLLKNTTTVISNVIGPLQQMALDNHPISGLYFTIVGTPQCYGNDVELY-G 427
           G +  A+++H  LKN++  ISN+IGP++Q+AL NHP  G+YF ++G+P+     +  Y G
Sbjct: 373 GPEVTARYIHGTLKNSSMTISNMIGPMEQLALANHPCRGIYFMVLGSPESMTISILSYMG 432

Query: 428 KLRVAFRTEKDFIDAHKLNCCMEDAFEKIFEAANDIP 456
           K+R+A  TEK FID  K N C+E+AF+++FEAA   P
Sbjct: 433 KVRIAVGTEKGFIDPRKFNACIENAFQRVFEAAVGTP 468

BLAST of IVF0008225 vs. ExPASy TrEMBL
Match: A0A1Q3CK04 (Diacylglycerol O-acyltransferase OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_24056 PE=4 SV=1)

HSP 1 Score: 447.2 bits (1149), Expect = 8.2e-122
Identity = 223/458 (48.69%), Postives = 312/458 (68.12%), Query Frame = 0

Query: 8   QPLSPIAEYLSSPMLSLSVLAILEFQVPIHTLDLAPNLRNLFLPINPRFSSILVTNEQGE 67
           QP+SP  +Y +S +LS+S+LA+LEF++P+H L     L+N+FLPINPRFSSI+V  ++GE
Sbjct: 10  QPVSPTGQYFTSTVLSISILAVLEFKIPLHALSTMLLLKNVFLPINPRFSSIMVRGKKGE 69

Query: 68  KKWKRVNVEVEEHVKDPIFPPGLTIESYKAHFDEYLTKISMEEFRPDKPLWETHLFKYPT 127
           K+WKRV V++E HV  PIFP GLT +SY+ +  +YL++I++E F   +PLWE H+ KYPT
Sbjct: 70  KQWKRVEVKLENHVNHPIFPAGLTPDSYEQYLTDYLSRIALERFPQSQPLWEVHIIKYPT 129

Query: 128 SPTVAGTMILKLHHSLGDGYSLMGVVLSCLHTASDPNVPLTFPSRR----QRRFNNGVVK 187
               AG++I KLHH+LGDGYSLMG +LSCLH A DP++ LTFPS R        N  +VK
Sbjct: 130 I-NAAGSVIFKLHHALGDGYSLMGALLSCLHRADDPSLSLTFPSLRLLSKTGEENKRIVK 189

Query: 188 SLTGIVSAVCRTASDLGWSVFKGI---DEKTPIRSGREGLQFMPMNTFTVSFSLNDIKKI 247
            +    S V  T  D GW + K     D++T IRSG  G++F P+   T++FSL+ IK+I
Sbjct: 190 RVQKRFSWVINTVRDFGWGLLKSSLVEDDRTLIRSGDAGVEFKPITITTITFSLDHIKQI 249

Query: 248 KTKLQVTVNDVITGIIFMGLRLYMEEIEKNSGEVAATALTIVNTRVIGNYRSATEMAKPE 307
           K KL VT+NDVITG IF G RLYM+   +   +   TAL ++NTR I  Y+S  EM  P+
Sbjct: 250 KYKLGVTINDVITGTIFFGTRLYMQTASQKLKDEHTTALVLLNTRDIDGYKSVKEMVNPD 309

Query: 308 SKGLWGNQLSYLEIMIPKMTNV--TNPLEFVMAAHRSINNKRTSFTIHVVAHLLNLLRKL 367
           +  LWGN+ ++L + +PK+ +   TNPL+F++ A ++I  KR+S  + +   LL  L+  
Sbjct: 310 ADSLWGNKFAFLHVSLPKLIDAEFTNPLKFILKARKNIKRKRSSLAVILTGRLLEALKSY 369

Query: 368 RGHQEVAKFLHNLLKNTTTVISNVIGPLQQMALDNHPISGLYFTIVGTPQCYGNDVELY- 427
           R  +  A+++H+ LKN++  ISNVIGP+++MALDNHPI GLYF +VG PQ     V  Y 
Sbjct: 370 RSPEVAARYIHSTLKNSSMTISNVIGPVEEMALDNHPIKGLYFMVVGVPQNLTISVLSYM 429

Query: 428 GKLRVAFRTEKDFIDAHKLNCCMEDAFEKIFEAANDIP 456
           GKLRVA  TEK FID  K   C+E A++K+ +AA++IP
Sbjct: 430 GKLRVAVGTEKGFIDPQKFKACIEIAYQKMLKAASEIP 466

BLAST of IVF0008225 vs. NCBI nr
Match: XP_008455536.1 (PREDICTED: O-acyltransferase WSD1-like [Cucumis melo] >KAA0031532.1 O-acyltransferase WSD1-like [Cucumis melo var. makuwa] >TYK06985.1 O-acyltransferase WSD1-like [Cucumis melo var. makuwa])

HSP 1 Score: 878 bits (2269), Expect = 0.0
Identity = 447/456 (98.03%), Postives = 448/456 (98.25%), Query Frame = 0

Query: 1   MDSSLLQQPLSPIAEYLSSPMLSLSVLAILEFQVPIHTLDLAPNLRNLFLPINPRFSSIL 60
           MDSSLLQQPLSPIAEYLSSPMLSLSVLAILEFQVPIHTLDLAPNLRNLFLPINPRFSSIL
Sbjct: 1   MDSSLLQQPLSPIAEYLSSPMLSLSVLAILEFQVPIHTLDLAPNLRNLFLPINPRFSSIL 60

Query: 61  VTNEQGEKKWKRVNVEVEEHVKDPIFPPGLTIESYKAHFDEYLTKISMEEFRPDKPLWET 120
           VTNEQGEKKWKRVNVEVEEHVKDPIFPPGLTIESYKAHFDEYLTKISMEEFRPDKPLWET
Sbjct: 61  VTNEQGEKKWKRVNVEVEEHVKDPIFPPGLTIESYKAHFDEYLTKISMEEFRPDKPLWET 120

Query: 121 HLFKYPTSPTVAGTMILKLHHSLGDGYSLMGVVLSCLHTASDPNVPLTFPSRRQRRFNNG 180
           HLFKYPTSPTVAGTMILKLHHSLGDGYSLMGVVLSCLHTASDPNVPLTFPSRRQRRFNNG
Sbjct: 121 HLFKYPTSPTVAGTMILKLHHSLGDGYSLMGVVLSCLHTASDPNVPLTFPSRRQRRFNNG 180

Query: 181 VVKSLTGIVSAVCRTASDLGWSVFKGIDEKTPIRSGREGLQFMPMNTFTVSFSLNDIKKI 240
           VVKSLTGIVSAVCRTASDLGWSVFKGIDEKTPIRSGREGLQFMPMNTFTVSFSLNDIKKI
Sbjct: 181 VVKSLTGIVSAVCRTASDLGWSVFKGIDEKTPIRSGREGLQFMPMNTFTVSFSLNDIKKI 240

Query: 241 KTKLQVTVNDVITGIIFMGLRLYMEEIEKNSGEVAATALTIVNTRVIGNYRSATEMAKPE 300
           KTKLQVTVNDVITGIIFMGLRLYMEEIEKNSGEVAATALTIVNTRVIGNYRSATEMAKPE
Sbjct: 241 KTKLQVTVNDVITGIIFMGLRLYMEEIEKNSGEVAATALTIVNTRVIGNYRSATEMAKPE 300

Query: 301 SKGLWGNQLSYLEIMIPKMTNVTNPLEFVMAAHRSINNKRTSFTIHVVAHLLNLLRKLRG 360
           SKGLWGNQLSYLEIMIPKMTNVTNPLEFVMAAHRSINNKRTSFTIHVVAHLLNLLRKLRG
Sbjct: 301 SKGLWGNQLSYLEIMIPKMTNVTNPLEFVMAAHRSINNKRTSFTIHVVAHLLNLLRKLRG 360

Query: 361 HQEVAKFLHNLLKNTTTVISNVIGPLQQMALDNHPISGLYFTIVGTPQCYGNDVELY-GK 420
           HQEVAKFLHNLLKNTTTVISNVIGPLQQMALDNHPISGLYFTIVGTPQ     +  Y GK
Sbjct: 361 HQEVAKFLHNLLKNTTTVISNVIGPLQQMALDNHPISGLYFTIVGTPQSVTVTMLSYMGK 420

Query: 421 LRVAFRTEKDFIDAHKLNCCMEDAFEKIFEAANDIP 455
           LRVAFRTEKDFIDAHKLNCCMEDAFEKIFEAANDIP
Sbjct: 421 LRVAFRTEKDFIDAHKLNCCMEDAFEKIFEAANDIP 456

BLAST of IVF0008225 vs. NCBI nr
Match: XP_031280304.1 (O-acyltransferase WSD1-like [Pistacia vera])

HSP 1 Score: 470 bits (1210), Expect = 7.55e-160
Identity = 235/460 (51.09%), Postives = 327/460 (71.09%), Query Frame = 0

Query: 4   SLLQQPLSPIAEYLSSPMLSLSVLAILEFQVPIHTLDLAPNLRNLFLPINPRFSSILVTN 63
           S+ ++P+SP  +YL+S  LSLS+L +LEF+VPI  L +   +++LFLPINPRFSSI+V +
Sbjct: 3   SITKEPVSPTGQYLNSSALSLSILGVLEFEVPIDNLPVMSLIKDLFLPINPRFSSIMVVD 62

Query: 64  EQGEKKWKRVNVEVEEHVKDPIFPPGLTIESYKAHFDEYLTKISMEEFRPDKPLWETHLF 123
           E GEK+WKRV VE+  HVK PIFP GL+ E Y  +FD+Y++KI ME+FR  +PLWE H+ 
Sbjct: 63  ENGEKQWKRVEVELHNHVKMPIFPSGLSPEFYDKYFDDYISKIGMEQFRRTRPLWEIHVI 122

Query: 124 KYPTSPTVAGTMILKLHHSLGDGYSLMGVVLSCLHTASDPNVPLTFPS-----RRQRRFN 183
           KYPT+ T A  MI KLHHSLGDG+SLMG +LSCL  A +P++PLTFPS      R    N
Sbjct: 123 KYPTT-TAAAVMIFKLHHSLGDGFSLMGALLSCLQRADNPSLPLTFPSVHLHSNRNGSNN 182

Query: 184 NGVVKSLTGIVSAVCRTASDLGWSVFKGI---DEKTPIRSGREGLQFMPMNTFTVSFSLN 243
           N ++KS++ ++S +  T SD  WS+ K     D+KTPIRSG  G++F P  T T+ FSL+
Sbjct: 183 NNILKSVSKVLSVISMTVSDFSWSIIKSTLVEDDKTPIRSGDGGIEFRPTTTATMIFSLD 242

Query: 244 DIKKIKTKLQVTVNDVITGIIFMGLRLYMEEIEKNSGEVAATALTIVNTRVIGNYRSATE 303
            I++IKTKL+ T+NDVITGIIF+G RLYM+E+ ++S +  +TA+ ++NTRV  +Y S  +
Sbjct: 243 KIRQIKTKLRATINDVITGIIFLGTRLYMQEMSQDSSKAQSTAVILLNTRVFRSYESVKD 302

Query: 304 MAKPESKGLWGNQLSYLEIMIPKMTN---VTNPLEFVMAAHRSINNKRTSFTIHVVAHLL 363
           M KP +K  WGN+ ++L + IP +T+    +NPLEFV+ A + I+ K+ S  +++ A LL
Sbjct: 303 MVKPNAKAPWGNRFAFLHVSIPHLTDDATSSNPLEFVLKAQQIISAKKNSLAVYLTAQLL 362

Query: 364 NLLRKLRGHQEVAKFLHNLLKNTTTVISNVIGPLQQMALDNHPISGLYFTIVGTPQCYGN 423
            +L+K +G +  A F+H  LKNT+  ISN++GP++QMAL NHP+ GLYFT+VG PQ    
Sbjct: 363 EILKKFKGPEAAAGFIHGTLKNTSMGISNLMGPVEQMALANHPVRGLYFTVVGVPQSLCI 422

Query: 424 DVELY-GKLRVAFRTEKDFIDAHKLNCCMEDAFEKIFEAA 451
            +  Y GKLRVA   EK FID  K  CC+E+AF+ I +AA
Sbjct: 423 TIVSYTGKLRVAVGAEKGFIDTPKFKCCVENAFQMILKAA 461

BLAST of IVF0008225 vs. NCBI nr
Match: XP_034680668.1 (O-acyltransferase WSD1-like [Vitis riparia])

HSP 1 Score: 448 bits (1152), Expect = 1.02e-150
Identity = 218/451 (48.34%), Postives = 317/451 (70.29%), Query Frame = 0

Query: 8   QPLSPIAEYLSSPMLSLSVLAILEFQVPIHTLDLAPNLRNLFLPINPRFSSILVTNEQGE 67
           QP+SP  +Y +S +L +S+++ILE  +PI  L     L+++FLPINPRFSS++V ++ G 
Sbjct: 13  QPVSPTGQYFNSSVLQISIMSILELDIPIDDLPTLSLLKDVFLPINPRFSSLMVEDKNGV 72

Query: 68  KKWKRVNVEVEEHVKDPIFPPGLTIESYKAHFDEYLTKISMEEFRPDKPLWETHLFKYPT 127
           K+WKRV V++E+HV  PIFP GL+ ESY  +FD+YLTKI+M+EF   +PLWETH+ KYPT
Sbjct: 73  KQWKRVEVKLEDHVNVPIFPDGLSPESYDDYFDDYLTKIAMKEFPQSRPLWETHIIKYPT 132

Query: 128 SPTVAGTMILKLHHSLGDGYSLMGVVLSCLHTASDPNVPLTFPSRR-QRRFNNG--VVKS 187
           S   AGT++ KLHH+LGDG+SLMG +LSCL  A +PN+P+TFPS +     + G  ++++
Sbjct: 133 S-NAAGTVVFKLHHALGDGFSLMGALLSCLQRADNPNLPITFPSFKVSSNLDKGRSIIRA 192

Query: 188 LTGIVSAVCRTASDLGWSVFKGI---DEKTPIRSGREGLQFMPMNTFTVSFSLNDIKKIK 247
           +   +SA   T SD GWS+ K     D++TPIRSG  G++F P++  T+SFSL++I+KIK
Sbjct: 193 VPRALSAAFNTVSDFGWSLLKSTVVEDDRTPIRSGDPGVEFRPLSITTISFSLDNIQKIK 252

Query: 248 TKLQVTVNDVITGIIFMGLRLYMEEIEKNSGEVAATALTIVNTRVIGNYRSATEMAKPES 307
            KL VT+NDV+TGIIF G RLYM+ +   S    +TAL ++NTRVI  Y+S  EM   +S
Sbjct: 253 AKLGVTINDVLTGIIFFGTRLYMQSMNHASRNANSTALVLLNTRVISGYKSVKEMTASDS 312

Query: 308 KGLWGNQLSYLEIMIPKMTNV--TNPLEFVMAAHRSINNKRTSFTIHVVAHLLNLLRKLR 367
              WGNQ ++L + +P++ +   T+PL+FV  A ++I  KR S  +H+   LL  LRK R
Sbjct: 313 SSQWGNQFAFLHVTLPELADAKFTSPLDFVAKAQQTIQRKRNSLAVHLTGRLLETLRKYR 372

Query: 368 GHQEVAKFLHNLLKNTTTVISNVIGPLQQMALDNHPISGLYFTIVGTPQCYGNDVELY-G 427
           G +  A+++H  LKN++  ISN+IGP++Q+ALDNHP  G+YF ++G+P+     +  Y G
Sbjct: 373 GPEVTARYIHGTLKNSSMAISNMIGPIEQVALDNHPCRGIYFMVLGSPESMTISIVSYMG 432

Query: 428 KLRVAFRTEKDFIDAHKLNCCMEDAFEKIFE 449
           K+R+A  TEK FID  K N C+EDAF+++ +
Sbjct: 433 KVRIAVGTEKGFIDPRKFNACIEDAFQRVLK 462

BLAST of IVF0008225 vs. NCBI nr
Match: RVX09350.1 (O-acyltransferase WSD1 [Vitis vinifera])

HSP 1 Score: 447 bits (1149), Expect = 2.89e-150
Identity = 218/457 (47.70%), Postives = 318/457 (69.58%), Query Frame = 0

Query: 8   QPLSPIAEYLSSPMLSLSVLAILEFQVPIHTLDLAPNLRNLFLPINPRFSSILVTNEQGE 67
           QP+SP  +Y +S +L +S+++ILE  +PI  L     L+++FLPINPRFSS++V ++ G 
Sbjct: 13  QPVSPTGQYFNSSVLQISIMSILELDIPIDDLPTLSLLKDVFLPINPRFSSLMVEDKNGV 72

Query: 68  KKWKRVNVEVEEHVKDPIFPPGLTIESYKAHFDEYLTKISMEEFRPDKPLWETHLFKYPT 127
           K+WKRV V++++HV  P+FP GL+ ESY  +FD+YLTKI+M+EF   +PLWE H+ KYPT
Sbjct: 73  KQWKRVEVKLQDHVNVPVFPDGLSPESYDDYFDDYLTKIAMKEFPQSRPLWEIHIIKYPT 132

Query: 128 SPTVAGTMILKLHHSLGDGYSLMGVVLSCLHTASDPNVPLTFPS-RRQRRFNNG--VVKS 187
           S   AGT++ KLHH+LGDG+SLMG +LSCL  A +PN+P+TFPS +     + G  ++++
Sbjct: 133 S-NAAGTVVFKLHHALGDGFSLMGALLSCLQRADNPNLPITFPSLKASSNLDKGRSIIRA 192

Query: 188 LTGIVSAVCRTASDLGWSVFKGI---DEKTPIRSGREGLQFMPMNTFTVSFSLNDIKKIK 247
           +   +SA   T SD GWS+ K     D++TPIRSG  G++F P++  T+SFSL+ I+KIK
Sbjct: 193 VPRALSAAFNTVSDFGWSLLKSTVVEDDRTPIRSGDPGVEFRPLSIATISFSLDYIQKIK 252

Query: 248 TKLQVTVNDVITGIIFMGLRLYMEEIEKNSGEVAATALTIVNTRVIGNYRSATEMAKPES 307
            KL VT+NDV+TGIIF G RLYM+ +   S    +TAL ++NTRVI  Y+S  EM   +S
Sbjct: 253 AKLGVTINDVLTGIIFFGTRLYMQSMNHASRNANSTALVLLNTRVISGYKSLKEMTASDS 312

Query: 308 KGLWGNQLSYLEIMIPKMTNV--TNPLEFVMAAHRSINNKRTSFTIHVVAHLLNLLRKLR 367
              WGNQ ++L + +P++ +   T+PL+FV  A ++I  KR S  +H+   LL  LRK R
Sbjct: 313 SSQWGNQFAFLHVTLPELADAKFTSPLDFVAKAQQTIQRKRNSLAVHLTGRLLETLRKYR 372

Query: 368 GHQEVAKFLHNLLKNTTTVISNVIGPLQQMALDNHPISGLYFTIVGTPQCYGNDVELY-G 427
           G +  A+++H  LKN++  I+N+IGP +Q+ALDNHP  G+YF ++G+P+     +  Y G
Sbjct: 373 GPEVTARYIHGTLKNSSMAITNMIGPTEQVALDNHPCRGIYFMVLGSPESMTISIVSYMG 432

Query: 428 KLRVAFRTEKDFIDAHKLNCCMEDAFEKIFEAANDIP 455
           K+R+A  TEK FID  K N C+E+AF+++FEAA   P
Sbjct: 433 KVRIAVGTEKGFIDPRKFNACIENAFQRVFEAALGTP 468

BLAST of IVF0008225 vs. NCBI nr
Match: XP_002274522.1 (PREDICTED: O-acyltransferase WSD1 [Vitis vinifera] >XP_019078819.1 PREDICTED: O-acyltransferase WSD1 [Vitis vinifera])

HSP 1 Score: 444 bits (1143), Expect = 1.05e-149
Identity = 219/457 (47.92%), Postives = 318/457 (69.58%), Query Frame = 0

Query: 8   QPLSPIAEYLSSPMLSLSVLAILEFQVPIHTLDLAPNLRNLFLPINPRFSSILVTNEQGE 67
           QP+SP  +Y +S +L +S+++ILE  +PI        L+++FLPINPRFSS++V +++G 
Sbjct: 13  QPVSPTGQYFNSSVLQVSIMSILESDIPIDDSPTLSLLKDVFLPINPRFSSLMVEDKKGV 72

Query: 68  KKWKRVNVEVEEHVKDPIFPPGLTIESYKAHFDEYLTKISMEEFRPDKPLWETHLFKYPT 127
           K WKRV V++E+HV  PIFP GL+ ESY  +FD+YLTKI+M+EF   +PLWE H+ KYPT
Sbjct: 73  KHWKRVEVKLEDHVNVPIFPDGLSPESYDDYFDDYLTKIAMKEFPQSRPLWEIHIIKYPT 132

Query: 128 SPTVAGTMILKLHHSLGDGYSLMGVVLSCLHTASDPNVPLTFPSRR-QRRFNNG--VVKS 187
           S   AGT++ KLHH+LGDG+SLMG +LSCL  A +PN+P+TFPS +     ++G  ++++
Sbjct: 133 S-NAAGTVVFKLHHALGDGFSLMGALLSCLQRADNPNLPITFPSFKVSSNLDSGRSIIRA 192

Query: 188 LTGIVSAVCRTASDLGWSVFKGI---DEKTPIRSGREGLQFMPMNTFTVSFSLNDIKKIK 247
           +   +SA   T SD GW + K     D++TPIRSG  G++F PM+  T+SFSL++I+KIK
Sbjct: 193 VPRALSAAFNTVSDFGWGLLKSTAVEDDRTPIRSGDPGVEFRPMSIATISFSLDNIQKIK 252

Query: 248 TKLQVTVNDVITGIIFMGLRLYMEEIEKNSGEVAATALTIVNTRVIGNYRSATEMAKPES 307
            KL VT+NDV+TGIIF G RLYM+ +   S    +TAL ++NTRVI  Y+S  EM   +S
Sbjct: 253 AKLGVTINDVLTGIIFFGTRLYMQSMNHASRNANSTALVLLNTRVISGYKSIKEMTASDS 312

Query: 308 KGLWGNQLSYLEIMIPKMTNV--TNPLEFVMAAHRSINNKRTSFTIHVVAHLLNLLRKLR 367
              WGNQ ++L + +P++ +   T+PL+FV  A ++I  KR S  +H+   LL  LRK R
Sbjct: 313 SSQWGNQFAFLHVTLPELADAKFTSPLDFVAKAQQTIQRKRNSLAVHLTGRLLETLRKYR 372

Query: 368 GHQEVAKFLHNLLKNTTTVISNVIGPLQQMALDNHPISGLYFTIVGTPQCYGNDVELY-G 427
           G +  A+++H  LKN++  ISN+IGP++Q+AL NHP  G+YF ++G+P+     +  Y G
Sbjct: 373 GPEVTARYIHGTLKNSSMTISNMIGPMEQLALANHPCRGIYFMVLGSPESMTISILSYMG 432

Query: 428 KLRVAFRTEKDFIDAHKLNCCMEDAFEKIFEAANDIP 455
           K+R+A  TEK FID  K N C+E+AF+++FEAA   P
Sbjct: 433 KVRIAVGTEKGFIDPRKFNACIENAFQRVFEAAVGTP 468

BLAST of IVF0008225 vs. TAIR 10
Match: AT3G49190.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 171.8 bits (434), Expect = 1.3e-42
Identity = 131/474 (27.64%), Postives = 225/474 (47.47%), Query Frame = 0

Query: 7   QQPLSPIAEYLSSPMLSLSVLAILEFQVPIHTLDLAPNLRNLFLPINPRFSSILVTNEQG 66
           +QPLSP A    +P  + ++++++ F+  +         +  F+  +PRFSS LVT+E G
Sbjct: 17  EQPLSPAARLFHAPEFNCNIISVIGFKSKLDPCVFIRGFKESFIR-HPRFSSKLVTDENG 76

Query: 67  E-KKWKRVNVEVEEHVKDPIFPPGLTIESYKAHFDEYLTKISMEEFRPDKPLWETHLFKY 126
           + ++W R NV VE+H   P   P   IE+  A  ++Y++ +        +PLWE HL   
Sbjct: 77  QNQRWVRTNVVVEDHFIVPKIKP-QNIENSNAFLEDYVSDLMKIPLDTSRPLWELHLLDL 136

Query: 127 PTSPTVAGTMILKLHHSLGDGYSLMGVVLSCLHTASD----PNVP-----------LTFP 186
            TS       +LK+HHS+GDG S+M +VL+C+   S+    P++P           LT  
Sbjct: 137 KTS-DAENVAVLKIHHSVGDGMSIMSLVLACMRKTSNPDELPSLPYQYRSSSRSSLLTTG 196

Query: 187 SRRQRRFNNGVVKSLTGIV---SAVCRTASDLGWSVFKGIDEKTPIRSGREGLQFMPMNT 246
           SR   R    V    T ++   + VC     +  ++F   D +TPI+      +   +  
Sbjct: 197 SRSDSRLLWLVKVIWTAVILGLNTVCDALEFIVTTLFVK-DTETPIKGDFLSTKSKQLRL 256

Query: 247 FTVSFSLNDIKKIKTKLQVTVNDVITGIIFMGL-----RLYMEEIEKNSGE------VAA 306
              + SL+DIK  K  + +T+NDV+ G+   GL     R Y EE  KN  +      +  
Sbjct: 257 VHRTVSLDDIKLTKNAMNMTINDVVLGVTQAGLSRYLARRYGEEETKNRKQKKLPKRIRL 316

Query: 307 TALTIVNTRVIGNYRSATEMAKPESKGLWGNQLSYLEIMIP-KMTNVTNPLEFVMAAHRS 366
            +  +VN R     +   +M +  SK  WGN   Y  ++ P  +    +PLE +  A ++
Sbjct: 317 RSALLVNLRPTTGIQDLADMMEKGSKCRWGNWFGY--VVFPFSIALRDDPLEHLEIAQKT 376

Query: 367 INNKRTSFTIHVVAHLLNLLRKLRGHQEVAKFLHNLLKNTTTVISNVIGPLQQMALDNHP 426
           I+ K+ S+   +      ++ KL G +  A  ++ ++ NTT   SN++GP+++++   HP
Sbjct: 377 ISRKKNSYGAMLTYIFCRIIVKLLGIKVAASIINRMVSNTTMTFSNMVGPVEEVSFYGHP 436

Query: 427 ISGLYFTIVGTPQCYGNDVELY-GKLRVAFRTEKDFI-DAHKLNCCMEDAFEKI 448
           I+    +  G P       + Y  K+ +    +   I D H+L    E++   I
Sbjct: 437 ITYFASSAYGHPHALTIHCQSYMNKMTITLIVDPTVISDPHRLCDDWEESLRSI 484

BLAST of IVF0008225 vs. TAIR 10
Match: AT5G53390.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 169.5 bits (428), Expect = 6.3e-42
Identity = 130/455 (28.57%), Postives = 218/455 (47.91%), Query Frame = 0

Query: 7   QQPLSPIAEYLSSPMLSLSVLAILEFQVPIHTLDLAPNLRNLFLPINPRFSSILVTNEQG 66
           ++PLSP +   +SP  + +++  +  +V   T  +   L +  L  +PRFSSIL  N   
Sbjct: 14  EEPLSPCSRLFNSPDFNCAIIVTMGSKVKGDTPAIIHGLEHT-LVNHPRFSSILEMNNGK 73

Query: 67  EKKWKRVNVEVEEHVKDPIFPPGLTIESYKAHFDEYLTKISMEEFRPDKPLWETHLFKYP 126
           + +W R  V+VEEHV  P   P   IE+   + ++Y++K++       KPLWE HL    
Sbjct: 74  KPRWVRTKVKVEEHVIVPDVDP--DIENPDQYLEDYISKLTTIPMDLSKPLWEMHLLGVK 133

Query: 127 TSPTVAGTMILKLHHSLGDGYSLMGVVLSCLHTASDPNVPLTFPSRRQR---RFNNG--- 186
           TS       ILK+HHSLGDG SLM ++L+C    SDP    T    ++R     N+G   
Sbjct: 134 TS-NAESYAILKIHHSLGDGMSLMSLLLACTRKTSDPEALPTVAVHKKRFGPSCNSGFFN 193

Query: 187 ----VVKSLTGIVSAVCRTASDL---GWSVFKGIDEKTPIRSGREGLQFMPMNTFTVSFS 246
               +   L  I+  +  T  D+     ++F   D +TP+ + + G + +P        S
Sbjct: 194 KIWWLFVGLWFILRLLFNTFVDILMFALTIFVLRDTETPLLA-KPGSELIPKRFVHRIIS 253

Query: 247 LNDIKKIKTKLQVTVNDVITGIIFMGL-----RLYMEEIEKNSGE----VAATALTIVNT 306
            +D+K +K  +++TVNDV+ G+   GL     R Y +E    S E    +   +  ++N 
Sbjct: 254 FDDVKLVKNAMKMTVNDVLLGVTQAGLSRYLSRKYDQEATPKSKESMRRIRLRSAIMINL 313

Query: 307 RVIGNYRSATEMAKPESKGLWGNQLSYLEIMIPKMTNV-TNPLEFVMAAHRSINNKRTSF 366
           R      +  +M   +SK  WGN   Y  I++P    + T+PLE+V  A  +I+ K+ S 
Sbjct: 314 RPNAGIEALADMMAKKSKCRWGNLFGY--ILLPFSVGLETDPLEYVRQAKATIDRKKHSL 373

Query: 367 TIHVVAHLLNLLRKLRGHQEVAKFLHNLLKNTTTVISNVIGPLQQMALDNHPISGLYFTI 426
                     L+ K+ G +     +  ++ +TT   SNV+GP +++    HP++ +   +
Sbjct: 374 EAVFSMAFFKLILKVLGLKASVVLVRKVIHSTTLSFSNVVGPKEEITFHGHPLNYISPCV 433

Query: 427 VGTPQCYGNDVELY-GKLRVAFRTEKDFI-DAHKL 437
            G P       + Y  K+ ++   +   I D HK+
Sbjct: 434 FGHPHALTLHFQTYANKVIISVTADPTVIPDPHKM 461

BLAST of IVF0008225 vs. TAIR 10
Match: AT3G49200.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 164.1 bits (414), Expect = 2.7e-40
Identity = 128/476 (26.89%), Postives = 221/476 (46.43%), Query Frame = 0

Query: 7   QQPLSPIAEYLSSPMLSLSVLAILEFQVPIHTLDLAPNLRNLFLPINPRFSSILV---TN 66
           +QPLSP A    +P  +  +++++  +  I    +   ++   +  +PRFSS LV    N
Sbjct: 25  EQPLSPAARLFHAPEFNCYIISVVGLKNKIEPDMIIEGIKQTLMR-HPRFSSKLVNNCNN 84

Query: 67  EQGEKKWKRVNVEVEEHVKDP-IFPPGLTIESYKAHFDEYLTKISMEEFRPDKPLWETHL 126
            + E+KW R NV VE+HV  P I    +   +     + Y++ ++       KPLWE HL
Sbjct: 85  NRQEQKWVRTNVVVEDHVIIPKIQTQHIENANADVFLESYVSDLTTIPLDTSKPLWEVHL 144

Query: 127 FKYPTSPTVAGTMILKLHHSLGDGYSLMGVVLSCLHTASDPNVPLTFPSRRQRRFNNGVV 186
               TS       +L++HHSLGDG S+M +VL+C    S+PN   + P + +    +  +
Sbjct: 145 LDLKTS-DAENVAVLRIHHSLGDGMSMMSLVLACTRKTSNPNELPSLPYQNRPSSGSSSL 204

Query: 187 KSLTG------------------IVSAVCRTASDLGWSVFKGIDEKTPIRSGREGLQFMP 246
           K+ +                   +++ VC     +  ++F   D +TPI+   +  +   
Sbjct: 205 KTSSRCYSRFFWLVMVLWSAALLVLNTVCDALEFIATALFLK-DTETPIKGDFKLSKGKR 264

Query: 247 MNTFTVSFSLNDIKKIKTKLQVTVNDVITGIIFMGLRLYM-------EEIEKNSGE---- 306
           M     + SL+DIK IK  +++TVNDV+ G+   GL  Y+       EE ++NS      
Sbjct: 265 MCMVHRTVSLDDIKLIKNAMKMTVNDVVLGVSQAGLSQYLKRRYGEQEESKRNSSNIPKG 324

Query: 307 VAATALTIVNTRVIGNYRSATEMAKPESKGLWGNQLSYLEIMIPKMTNVTNPLEFVMAAH 366
           +   A  +VN R     +   +M    SK  WGN + Y+ I    +    +PL+ +  A 
Sbjct: 325 IRLRAALLVNLRPTTGIQDLADMMTKGSKCRWGNWIGYI-IFPFSIALCDDPLKHLRRAK 384

Query: 367 RSINNKRTSFTIHVVAHLLNLLRKLRGHQEVAKFLHNLLKNTTTVISNVIGPLQQMALDN 426
            +I+ K+ S    +   +  +L    G Q  A  L+  L NTT   SN++GP+++++   
Sbjct: 385 STIDRKKNSLEAVLTFVVGKILLNTLGVQRAANVLNRALSNTTMSFSNLVGPVEEISFYG 444

Query: 427 HPISGLYFTIVGTPQCYGNDVELY-GKLRVAFRTEKDFI-DAHKLNCCMEDAFEKI 448
           H ++ +  ++ G P       + Y  KL ++   +   I D HKL    E++   I
Sbjct: 445 HTVTYIAPSVYGHPHALTMHFQSYMNKLTISLTVDPTVISDPHKLCDDWEESLRSI 496

BLAST of IVF0008225 vs. TAIR 10
Match: AT5G12420.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 163.3 bits (412), Expect = 4.5e-40
Identity = 129/477 (27.04%), Postives = 228/477 (47.80%), Query Frame = 0

Query: 7   QQPLSPIAEYLSSPMLSLSVLAILEFQVPIHTLDLAPNLRNLFLPINPRFSSILVTNEQG 66
           ++P+SP+A    SP + L  + I+ F+  I+  D+  +     +  +PRFSSIL  N   
Sbjct: 6   EEPVSPMARVFQSPGIDLCAVTIMGFKTKINP-DVVLDALKQNVSKHPRFSSILSDN--- 65

Query: 67  EKKWKRVNVEVEEHVKDPIFPPGLTIESYKAHFDEYLTKISMEEFRPDKPLWETHLFKYP 126
             KW    V VE+HV  P        E  ++  D+Y+++++M      +PLW+ H+    
Sbjct: 66  GAKWIETEVNVEDHVIVPYIDAEEIGEGGQSFIDDYMSRLTMIPLDRSRPLWDIHILNVK 125

Query: 127 TSPTVAGTMILKLHHSLGDGYSLMGVVLSCLHTASDPNV-PLTFPSRRQRRFNNGVVKSL 186
           TS   A    ++ HHSLGDG SL+ ++L+C H  SDP++     PS ++R   +  +K+ 
Sbjct: 126 TSEAEA-VGFIRSHHSLGDGMSLISLMLACTHKTSDPDMFSNAIPSMKRRATMSHSLKTK 185

Query: 187 TGIVSAVCRTASD--LGWSVFKGI-----------DEKTPIRSGREGLQFMPMNTFTVSF 246
              + ++    S   L W+    +           D KTP+++G + ++  P   +    
Sbjct: 186 GWFLRSIFTIGSTMRLLWNTTIDMLLLLATVLFLKDTKTPLKAGAD-VRSNPKRFYHRII 245

Query: 247 SLNDIKKIKTKLQVTVNDVITGIIFMGLRLYMEEI--EKNSGEVAATA------------ 306
           SL+DIK IK  + +T+NDV+ GI    L  Y+      K   + A T+            
Sbjct: 246 SLDDIKLIKNAMNMTINDVLFGITQASLSHYLNRQYGTKKEEDGALTSYRNNLPDGIRFR 305

Query: 307 -LTIVNTRVIGNYRSATEMAKPESKGLWGNQLSYLEIMIPKMTNV-TNPLEFVMAAHRSI 366
               VN R    ++   +M   +SK  WGN  S+  I +P    + T+PL ++  +   +
Sbjct: 306 VACTVNLRSDIGFKPLADMMVKDSKCRWGNYFSF--IFLPFTIGLQTDPLVYLKMSKSMM 365

Query: 367 NNKRTSFTIHVVAHLLNLLRKLRGHQEVAKFLHNLLKNTTTVISNVIGPLQQMALDNHPI 426
             K+ S+   +V  ++ ++ K+ G +  A+     ++NTTT +SNVIGP+++++   HP+
Sbjct: 366 ARKKHSYHAALVYFIIKIVLKVFGAKAAAELFDRPVRNTTTCVSNVIGPMEEISFRGHPV 425

Query: 427 SGLYFTIVGTPQCYGNDVELY-------GKLRVAFRTEKDFI-DAHKLNCCMEDAFE 446
           S +       P  YG+   L         K+ ++   +   I D HK+   ME++ +
Sbjct: 426 SYI------APSSYGHSHALLIHLMSYADKMIISLAYDPTVISDPHKICDDMEESLK 468

BLAST of IVF0008225 vs. TAIR 10
Match: AT5G22490.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 162.9 bits (411), Expect = 5.9e-40
Identity = 136/474 (28.69%), Postives = 224/474 (47.26%), Query Frame = 0

Query: 7   QQPLSPIAEYLSSPMLSLSVLAILEFQVPIHTLDLAPNLR-NLFLPINPRFSSILVTNEQ 66
           ++PLSP+A     P +   ++     +  I+   +  +L+ N+F   +PRF S+L   + 
Sbjct: 10  EEPLSPMARAFQEPSIDCGIVIKFGCKTKINPDVIVDSLKLNVFK--HPRFCSLL---DD 69

Query: 67  GEKKWKRVN-VEVEEHVKDPIFPPGLTIESYKAHFDEYLTKISMEEFRPDKPLWETHLFK 126
              KW R + V VEEHV  P   P LT E  +   ++Y++ I+M      KPLWE H+  
Sbjct: 70  DGTKWLRTDVVNVEEHVFVPDIDPKLTEEDVEWFVEDYISSITMIPLDRTKPLWEVHILN 129

Query: 127 YPTSPTVAGTMILKLHHSLGDGYSLMGVVLSCLHTASDPNVPLTFPSRRQRR-FNNGVVK 186
             TS   A   +++ HH+LGDG S++ ++L+     S+P    T P  + R  +N+    
Sbjct: 130 AKTSDAEA-ICVIRCHHALGDGVSILSLILASTRKTSEPEAFSTLPVPKCRESYNHRRGF 189

Query: 187 SLTGIVSAVCRTASDLGW-----------SVFKGIDEKTPIRSGREGLQFMPMNTFTVSF 246
           S   +V  VC T   L W           ++F   D  TP++ G+ G     +  F+   
Sbjct: 190 SFFRLVLVVCSTVR-LIWNTLVDSFLCMATIFFLKDTDTPLK-GKPG----AIKKFSHRI 249

Query: 247 -SLNDIKKIKTKLQVTVNDVITGIIFMGLRLYMEEIEKNSGEVAATALT----------- 306
            SL+DIK IK  +++T+NDV+ G+    L  Y+ +    + E A T+LT           
Sbjct: 250 VSLDDIKLIKNAMEMTINDVLLGVTEAALTRYLHQSYDKTNEEAGTSLTPNRQDLLDRIR 309

Query: 307 -----IVNTRVIGNYRSATEMAKPESKGLWGNQLSYLEIMIP-KMTNVTNPLEFVMAAHR 366
                +VN R  G+   A  MAK  SK  WGN +S   I+ P  +   ++PL ++     
Sbjct: 310 LRSLIVVNLRPTGSQSIADMMAK-GSKCRWGNYISV--ILFPFTIALQSDPLVYLSNVKS 369

Query: 367 SINNKRTSFTIHVVAHLLNLLRKLRGHQEVAKFLHNLLKNTTTVISNVIGPLQQMALDNH 426
            I+ K+ S   +++      + K  G      F   ++ NTT  ISN+ GP ++++   H
Sbjct: 370 MIDRKKNSLITYIIYTFSEFVIKAFGINVAVAFQRKIMLNTTMCISNLPGPTEEVSFHGH 429

Query: 427 PISGLYFTIVGTPQCYGNDVELY-GKLRVAFRTEKDFIDAHKLNCCMEDAFEKI 448
           PI+    +I G PQ        Y  K+ ++   +   IDAHKL   +E++ + +
Sbjct: 430 PIAYFAPSIYGLPQALTIHYLSYANKMIISVAVDPMIIDAHKLCDELEESLKNM 468

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M3B31.8e-4127.64Wax ester synthase/diacylglycerol acyltransferase 4 OS=Arabidopsis thaliana OX=3... [more]
Q5KS418.9e-4128.57Wax ester synthase/diacylglycerol acyltransferase 11 OS=Arabidopsis thaliana OX=... [more]
Q9M3B23.8e-3926.89Wax ester synthase/diacylglycerol acyltransferase 5 OS=Arabidopsis thaliana OX=3... [more]
Q94CK06.4e-3927.04Wax ester synthase/diacylglycerol acyltransferase 7 OS=Arabidopsis thaliana OX=3... [more]
Q9FK898.4e-3928.69Wax ester synthase/diacylglycerol acyltransferase 9 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A5A7SKE32.0e-25398.03Diacylglycerol O-acyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3C1V42.0e-25398.03Diacylglycerol O-acyltransferase OS=Cucumis melo OX=3656 GN=LOC103495688 PE=4 SV... [more]
A0A438JK811.1e-12347.70Diacylglycerol O-acyltransferase OS=Vitis vinifera OX=29760 GN=WSD1_5 PE=4 SV=1[more]
F6H5E65.7e-12347.92Diacylglycerol O-acyltransferase OS=Vitis vinifera OX=29760 GN=VIT_12s0028g03480... [more]
A0A1Q3CK048.2e-12248.69Diacylglycerol O-acyltransferase OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_2... [more]
Match NameE-valueIdentityDescription
XP_008455536.10.098.03PREDICTED: O-acyltransferase WSD1-like [Cucumis melo] >KAA0031532.1 O-acyltransf... [more]
XP_031280304.17.55e-16051.09O-acyltransferase WSD1-like [Pistacia vera][more]
XP_034680668.11.02e-15048.34O-acyltransferase WSD1-like [Vitis riparia][more]
RVX09350.12.89e-15047.70O-acyltransferase WSD1 [Vitis vinifera][more]
XP_002274522.11.05e-14947.92PREDICTED: O-acyltransferase WSD1 [Vitis vinifera] >XP_019078819.1 PREDICTED: O-... [more]
Match NameE-valueIdentityDescription
AT3G49190.11.3e-4227.64O-acyltransferase (WSD1-like) family protein [more]
AT5G53390.16.3e-4228.57O-acyltransferase (WSD1-like) family protein [more]
AT3G49200.12.7e-4026.89O-acyltransferase (WSD1-like) family protein [more]
AT5G12420.14.5e-4027.04O-acyltransferase (WSD1-like) family protein [more]
AT5G22490.15.9e-4028.69O-acyltransferase (WSD1-like) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR009721O-acyltransferase WSD1, C-terminalPFAMPF06974WS_DGAT_Ccoord: 305..447
e-value: 4.3E-27
score: 94.8
IPR004255O-acyltransferase, WSD1, N-terminalPFAMPF03007WES_acyltransfcoord: 97..252
e-value: 6.0E-9
score: 36.1
IPR045034O-acyltransferase WSD1-likePANTHERPTHR31650O-ACYLTRANSFERASE (WSD1-LIKE) FAMILY PROTEINcoord: 7..454
NoneNo IPR availablePANTHERPTHR31650:SF34O-ACYLTRANSFERASE WSD1-LIKEcoord: 7..454

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0008225.1IVF0008225.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019432 triglyceride biosynthetic process
biological_process GO:0045017 glycerolipid biosynthetic process
cellular_component GO:0005886 plasma membrane
molecular_function GO:0004144 diacylglycerol O-acyltransferase activity
molecular_function GO:0047196 long-chain-alcohol O-fatty-acyltransferase activity
molecular_function GO:0008374 O-acyltransferase activity