IVF0005443 (gene) Melon (IVF77) v1

Overview
NameIVF0005443
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionNudix hydrolase 20
Locationchr02: 15516015 .. 15521257 (-)
RNA-Seq ExpressionIVF0005443
SyntenyIVF0005443
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCTCCAAATCTCTCGCGCACAGCTTTCGTAATTTCTCAACTGTCCCTTCCTTTTGCATCGCTTCAACAATTTTCCGGACGACGTATACAACGATCTCTACATTTCGCCCAACAGTACACCGCTCGATATCGGTTGCCTCTCGTCCTGTATCGGTCACCCCCGGCAGTTTCACTTGGGACGACGTTGTCCATGTTTCTTCACCAGAAAGCTTTCAGGATGATCCTGCGGATTTGACGGGCTATTTCGAGAAGGTCAAGCTCTGCAATCGCGGATCAGTAGGTCTCTTTCTCCTTTTCTTTTCTTGTTTCGCTGTACAAACGAAAGATGTAAAGGCATTCCTTTCGGTACCAATTGTATACATTAGTTGGTTTTATATTTTTTAGATTCAGTGCAGGAAAAGCTATAGACTGATGCAATGTTGTCCCATGTCTGTCTGGAATGGACTAAAATTTTCTTACAGGATACGCAATCTGAATTTTTTCCGTTTGTCATCGATGGCCAAATTGTAGGCTACGTGCACCACGGGTGAGGCTTCTGTATCTTTATGGATGACCTTTGTTGGCTATGTTTGGTCGAAGCCACTTATTTACACTCCTTTTGTCAAAATTAGCTCGTGTGCGGTTGCTATATACAATGGATGGGTTTAATTGGTTTGTTTTAATGAGCTTTATGCAGTTTCGCTAAACACCTGCAGCAGTATCCGAATGTGTTTACCTTCTCTCAAGACGATTCTGTTAAATTTGGGGCTTACTTAACCTTGCATGAATCCTTGAAGACACCCGAAGATAGGACGCGTGCAGTTGGAGATGTAGTTAAATGCTTGGGTGAAGAAGTCATTCCGGGCACACGGAATGAGGTACTGGTTTATGATTCCAATTTAAAGCGATTATTACTTTACTTATCAATACCTGTTTGCAACTGTTTCACTGAAGGAGAAAATAAAAGGAAAGAATTTCCTTCTTCTGTTTAATACTGGAAATAAACTGTTTCTTTTTAGAAGGGTAATTTTAGAAGAAAACAATACTTCATGTGGCTAACTATGGATAACTGTAAACTATGTCAGCTTTTCCCTGTTACTTCGTCATTTGGTGCGCCGAACTTTTTTTCACTCGAACGTGCTGCAGCCCCTTATTTTGGAATAAAGGTGCAAAACGAATGTTCCCCTTTTACTGCTTTGTGGAGACTTTCAAAATTTTGTTCAATTTTTGTGAAATGCACTTCGGAAACTATATTTTGGTCCTTTTTGTTAGGCCCTTGAGCATAGTGGGCCATTTGATTTGTTGAATCTGTTCACACAAGTGTAGTTGTTAGGTCCAAATATAAGTGGAACTTGTTATATTTATGGTGACCACCTACTTAGTAGTTAGTTTTCTACAAGTTTCCTTGATACCCAAATGTTGTAGGGTCAGACACTTAAGTTGGCTTGGACATTCAAAGATATATAGAAAGAAATAAAAAATCTGAATATAAAGATTTGAAATAACAGATGATTTTGCAAACTTTCTGGTGCTAAAAATTCTACTTGGCAACTGCATTTCTTTTACATGTGCCAATCTCCTGCCTTGTTTAGAGGTTTAATCGTGGAATATGAGACGCACACAAAAAAACCGGTATTTTGTCCTCTTTTCTGCTTCTTCTGCAATCATTTTGACCACCTATATAACAAGACTAAAATCATTTTCATTGCTCTTCTTCCCTCCATTGATATTGGATTAAATTCAATCTTCCCTTGATTGAAACTCTGAAGTTTAAACTACACTAAATTCATGTTGGGAATAAATTTTGGTCATGGAAATAACCCTCACAGCAGTCCTATTAGTATTAGCTCTTGGTAAACACATTGCAAGATATCCTCCCTACATCCTCCAGGCTGTTGATCCTGGTGAGGCTAATCTTTTGGAGGCTTCTAGAGAAAACTGCAGGGCTCTGTGGTTACTTTCAAAGATTGTACAGCTTAGCCAACAGAAATATATAAAAGATGTATGGGGAAGTGGTATAAGAGGAAATTTTAATGCTGGTAGTATTTGAGAAGAGATTTCTTTACTTAAATCGATGACTTAATGACTTCTTGCATGATGAGAGTATTTAGGGCTTGACATTGACAACTAGATAAACCTAGAACCTTTCTTGAGATTTTATGATGTGCCACCTTACCATCGTGGTTGATACCTGTCTTGCAGTGTTTGCCAACAATAAACTACAATGGAAATTTAAGCTCCCAAAAGGATCAGATTTTTCTTATGCTCCCTAGCCCATGCCAAAGTTCATTCTACTAACAGTACCTAAAAGAAAAATATCTGTTTAGCCTTTTATTCCTCAGTGCTATGGTTCACTAGAAAATGATTGGTCATTCCTCAAAGTAGCTCAAGAGGGCAATATTAATTTTGCAGGTTAAGTGAAACTCTTTGAAGGGTTAAATATAGAGAATATCTCTCATGGTGTAAATGGAAAATTCATTTTGCATAGGGTAAAGCATACATCACTATAGCTGATATCTGAAGTTATTCACACACTTTGATCTACTTACAAAAGAAGTGGCTAGACATTGAAGGGAAATATATTGTTGAATGAGCATGTTTATTCTTCTTTTTGTCTTTCTTATAATCGTGAGTGTTTGGGCTAGCTTACGTGCACCTTGACTAATCTCACGAGACAACCTGTCTGAACCTACAATATTTAGGTATCAAGGAAACTCGTAGGAAACTAATTCCTAGGTAAGAGACCACTATGGATTGAACTCATGACATCTTAGTTAGTTATTGAGACTATGTCTCCTTTTTTATCATTTGGCTAACCCATGATGATTGTTGAATGTACATGTTGGAGACGGAAATTGGCGGCTTGGTTGTAGATTACTGAAAGTTAGTTTACTTTACTACTTGGTTTCTCTAACTTTTACCTGCCTTCTCTTGGTGCTTTTCTTAGCACAGGTTTATGGTGTTCATATGAATGGTTATGTTGAGAAGGAGGGAAAGAAGTTTTTGTGGGTTGCTAAAAGAAGCCAAACAAAGCCAACTTTTCCTGGAATGTTGGATCATTTAGTAGCTGGAGGGCTGGTATGGAATAAGTGATAGCTAACTGTTGCAAGTTTCCATACATGTGCTTCTGTCCCGTTAAAATATGAAAAATAAAGACTTTTCTGCTTTTTAAGATTGGATGTTATGGTGTGTTAGTTCCCTTAACCTTTTTCTTTTCTTCTGCTGGGTATTTGTAGTTATATAAATACTTTCCAGTGAGTTAATATATCATTTAGCCTTCAAACTCTTTTTCAGTTTTGGTTCATAATATTTTCCAAGTTGTAAGGGTTAATTGTACCCTTAAGATATCAAAATATTTTGAAGTACATGTTATTTGTCGATATTGATAAAGAATGGTGACATGGAATTATTTTTTTTTTATTTGTGCAAAAGTTTTTTGAATATTATCCTATTTAGCTAAAAAATCCAACCTCCTTATTTACTATTTTATAAGATAAGAATAGATAATATATTTTTACCCTGTTTTATATAGTTTCATTGATTGACTAAGGGCTTGATTGAACATTTTGATACCTATATAGATGTAAGCTTTAGAGGCTGAATTATATATTTGAACCTTTCTCGTTAGTAATATTACATCCTGCTCCTGCTATAAAAGAATCTGTTTGTATTTCAGTTTGCATCTCTAGTTTGATAAAGTTAGTATATACATACAGCAATTATTGAGTTTACTTTGTTTGACTGTCCATTTGGATAATAAAGATTAAACGTTATAAAAAATTAGACAAAGTTTTGATACTGCAGTTTTTTTTTTCTCGTTTGATGATTTACAACTCATCAATAAATTAATTGGTTAAAAAGCAATGTGTTATATGGAGGGGTGTGAAATATGATGATGTTCATAGGAGCAGTTTGAAAGTCTGCCTATGAATTTTAAAACGTAGTTATATATGCAAACCTTGATACTAATCAGAGGAAAAAGAGACAAATGTAAAGTTCGAAATGTGATCTTGACTGCTCAGTAGTGTACTTATCGTGCCTCCGTAGCTATTCCCTGACATCTTGATTTTGACAGCCTCAGGGCATCTCTTGTGGGGAGAATCTGATGAAAGAATGCAAGGAGGAAGCAGGCATACCGAGATCAATATCAAAAGAGTAATTTCCTATAAAGAATTTGACAATGGACACGAATGTAACCTCCATGTATTTCACAGTTTTGGTGGATCTCGTGGCTTCTTATGATGCTTTTGATTTAGGGCCATACCAGTTGGTGCCATTTCATACACAGACATCAAAGGTTATGGTTACAAGAGAGATGTTCAATTCTGTTACGATTTAAAGCTTCCTGAAAGCTTTGTACCGGAGAATCAAGGTTAGCTAGGCTGATAACTTTTCTTTACATCGCAATTCAATATTTAGCTTACAAAGTGTGGTACATTGCATTTGACTGGCTCCTAACTACTCTCTTATGCATGCAATTTGTTTTTTTTTTTTCATTACAAATTTTTGTAGTTTGAAATCTCTTGTCACTAACTCGCTGACTGTACTTTTGTTAGATGGAGAGGTTGAAGGATTCATGTTGTTGCCTGTGACAAATGTTGCAAATGTGATCCGGAGGACACAGTTCTTCAAGCCTAATTGTTCTCTTGTTATTATCAATTTCCTGTTTCGACATGGGTACGAAATATTATCTTCTTTGAATATACCTTTGTTCCTCAACCATTATTTTATTTAGTTACAACTCTATTTTAGGTTTTGGTTGTAAATATTGGTGGGTTCAACCAAATTTTCAGAAATAGTTATATTCCATGTTTTCATAATTTTTTAATACGTGGTTGTTTACTTTGGTTTAGATGAGCAGATTTTTATTCTGAAAACAATGAAAATGCATAAATGTTTTGAAAACATTCACTTCCTATGCCACAGAATTTAAACACATTCTGATACTTTCATAAATAAGTCCATAAAATACAATTAATTAATGAATTCATTTGATTCAGTAAATTTGAAGAAAAGAAATTAAACTTGCATTAGATACCAAACTAACTTTTATAAATCCGTTCTTTCCTCCATGTTTTGAATCTTTCTACACGTGTGTGTGTAAGCAACCCAGTATATGCTAATTACCACTCCTCAGGCTATCCTTATATAGTCCACAGTATTTTGTATTAATGCATTTAAAAACTTGCAGATACATTAAACCCGAAAGTTCGGGATACTTGGAGCTACTGCAGGGTTTGAGAAGTGGGACATGCTTCTGA

mRNA sequence

ATGGCCTCCAAATCTCTCGCGCACAGCTTTCGTAATTTCTCAACTGTCCCTTCCTTTTGCATCGCTTCAACAATTTTCCGGACGACGTATACAACGATCTCTACATTTCGCCCAACAGTACACCGCTCGATATCGGTTGCCTCTCGTCCTGTATCGGTCACCCCCGGCAGTTTCACTTGGGACGACGTTGTCCATGTTTCTTCACCAGAAAGCTTTCAGGATGATCCTGCGGATTTGACGGGCTATTTCGAGAAGGTCAAGCTCTGCAATCGCGGATCAGATACGCAATCTGAATTTTTTCCGTTTGTCATCGATGGCCAAATTGTAGGCTACGTGCACCACGGTTTCGCTAAACACCTGCAGCAGTATCCGAATGTGTTTACCTTCTCTCAAGACGATTCTGTTAAATTTGGGGCTTACTTAACCTTGCATGAATCCTTGAAGACACCCGAAGATAGGACGCGTGCAGTTGGAGATGTAGTTAAATGCTTGGGTGAAGAAGTCATTCCGGGCACACGGAATGAGCTTTTCCCTGTTACTTCGTCATTTGGTGCGCCGAACTTTTTTTCACTCGAACGTGCTGCAGCCCCTTATTTTGGAATAAAGGTTTATGGTGTTCATATGAATGGTTATGTTGAGAAGGAGGGAAAGAAGTTTTTGTGGGTTGCTAAAAGAAGCCAAACAAAGCCAACTTTTCCTGGAATGTTGGATCATTTAGTAGCTGGAGGGCTGCCTCAGGGCATCTCTTGTGGGGAGAATCTGATGAAAGAATGCAAGGAGGAAGCAGGCATACCGAGATCAATATCAAAAGAGGCCATACCAGTTGGTGCCATTTCATACACAGACATCAAAGGTTATGGTTACAAGAGAGATGTTCAATTCTGTTACGATTTAAAGCTTCCTGAAAGCTTTGTACCGGAGAATCAAGATGGAGAGGTTGAAGGATTCATGTTGTTGCCTGTGACAAATGTTGCAAATGTGATCCGGAGGACACAGTTCTTCAAGCCTAATTGTTCTCTTGTTATTATCAATTTCCTGTTTCGACATGGATACATTAAACCCGAAAGTTCGGGATACTTGGAGCTACTGCAGGGTTTGAGAAGTGGGACATGCTTCTGA

Coding sequence (CDS)

ATGGCCTCCAAATCTCTCGCGCACAGCTTTCGTAATTTCTCAACTGTCCCTTCCTTTTGCATCGCTTCAACAATTTTCCGGACGACGTATACAACGATCTCTACATTTCGCCCAACAGTACACCGCTCGATATCGGTTGCCTCTCGTCCTGTATCGGTCACCCCCGGCAGTTTCACTTGGGACGACGTTGTCCATGTTTCTTCACCAGAAAGCTTTCAGGATGATCCTGCGGATTTGACGGGCTATTTCGAGAAGGTCAAGCTCTGCAATCGCGGATCAGATACGCAATCTGAATTTTTTCCGTTTGTCATCGATGGCCAAATTGTAGGCTACGTGCACCACGGTTTCGCTAAACACCTGCAGCAGTATCCGAATGTGTTTACCTTCTCTCAAGACGATTCTGTTAAATTTGGGGCTTACTTAACCTTGCATGAATCCTTGAAGACACCCGAAGATAGGACGCGTGCAGTTGGAGATGTAGTTAAATGCTTGGGTGAAGAAGTCATTCCGGGCACACGGAATGAGCTTTTCCCTGTTACTTCGTCATTTGGTGCGCCGAACTTTTTTTCACTCGAACGTGCTGCAGCCCCTTATTTTGGAATAAAGGTTTATGGTGTTCATATGAATGGTTATGTTGAGAAGGAGGGAAAGAAGTTTTTGTGGGTTGCTAAAAGAAGCCAAACAAAGCCAACTTTTCCTGGAATGTTGGATCATTTAGTAGCTGGAGGGCTGCCTCAGGGCATCTCTTGTGGGGAGAATCTGATGAAAGAATGCAAGGAGGAAGCAGGCATACCGAGATCAATATCAAAAGAGGCCATACCAGTTGGTGCCATTTCATACACAGACATCAAAGGTTATGGTTACAAGAGAGATGTTCAATTCTGTTACGATTTAAAGCTTCCTGAAAGCTTTGTACCGGAGAATCAAGATGGAGAGGTTGAAGGATTCATGTTGTTGCCTGTGACAAATGTTGCAAATGTGATCCGGAGGACACAGTTCTTCAAGCCTAATTGTTCTCTTGTTATTATCAATTTCCTGTTTCGACATGGATACATTAAACCCGAAAGTTCGGGATACTTGGAGCTACTGCAGGGTTTGAGAAGTGGGACATGCTTCTGA

Protein sequence

MASKSLAHSFRNFSTVPSFCIASTIFRTTYTTISTFRPTVHRSISVASRPVSVTPGSFTWDDVVHVSSPESFQDDPADLTGYFEKVKLCNRGSDTQSEFFPFVIDGQIVGYVHHGFAKHLQQYPNVFTFSQDDSVKFGAYLTLHESLKTPEDRTRAVGDVVKCLGEEVIPGTRNELFPVTSSFGAPNFFSLERAAAPYFGIKVYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLVAGGLPQGISCGENLMKECKEEAGIPRSISKEAIPVGAISYTDIKGYGYKRDVQFCYDLKLPESFVPENQDGEVEGFMLLPVTNVANVIRRTQFFKPNCSLVIINFLFRHGYIKPESSGYLELLQGLRSGTCF
Homology
BLAST of IVF0005443 vs. ExPASy Swiss-Prot
Match: Q8VXZ0 (Nudix hydrolase 20, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NUDT20 PE=2 SV=1)

HSP 1 Score: 429.5 bits (1103), Expect = 3.9e-119
Identity = 212/350 (60.57%), Postives = 270/350 (77.14%), Query Frame = 0

Query: 26  FRTTYTTISTFRPTVHRSISVASR-PVSVTPGSFTWDDVVHVSSPESFQDDPADLTGYFE 85
           +R++  ++S  R +  R++S A+  P+S    SFTWDDV+     E    + +DLTG+FE
Sbjct: 32  WRSSSMSLSPLRHS--RALSAATTVPIS---SSFTWDDVIETGRAEY---NSSDLTGFFE 91

Query: 86  KVKLCNRGSDTQSEFFPFVIDGQIVGYVHHGFAKHLQQYPNVFTFSQDDSV--KFGAYLT 145
           K+  CNRGS+   EF PFVI+ QIVGY+H  F ++L+++ ++FTFSQ+ S   +   Y+T
Sbjct: 92  KINRCNRGSEKLGEFIPFVIEEQIVGYIHKRFTEYLREFHDIFTFSQNGSCPDRVDGYVT 151

Query: 146 LHESLKTPEDRTRAVGDVVKCLGEE-VIPGTRNELFPVTSSFGAPNFFSLERAAAPYFGI 205
           L+  L+ PEDRTRAV DV+K LG++ +IPG RNEL+PV  SF AP FFSLERAAAPYFGI
Sbjct: 152 LNLMLQKPEDRTRAVADVIKILGDKGIIPGIRNELYPVKPSFNAPVFFSLERAAAPYFGI 211

Query: 206 KVYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLVAGGLPQGISCGENLMKECKEE 265
           K YGVHMNGYVE++G+K LW+ KRS +K T+PGMLDHLVAGGLP GISCG NL+KEC+EE
Sbjct: 212 KGYGVHMNGYVERDGQKLLWIGKRSLSKSTYPGMLDHLVAGGLPHGISCGGNLVKECEEE 271

Query: 266 AGIPRSISKEAIPVGAISYTDIKGYGYKRDVQFCYDLKLPESFVPENQDGEVEGFMLLPV 325
           AGI R+I+  AI VGA+SY DI  Y +KRDV FCYDL+LPE FVP+NQDGEVE F L+PV
Sbjct: 272 AGISRAIADRAIAVGAVSYLDIDQYCFKRDVLFCYDLELPEDFVPKNQDGEVESFKLIPV 331

Query: 326 TNVANVIRRTQFFKPNCSLVIINFLFRHGYIKPESSGYLELLQGLRSGTC 372
             VA+VI++T FFK NCSLVII+FLFRHG+I+PESSGYL+L Q LR+  C
Sbjct: 332 AQVASVIKKTSFFKANCSLVIIDFLFRHGFIRPESSGYLDLYQRLRNRDC 373

BLAST of IVF0005443 vs. ExPASy Swiss-Prot
Match: P0C026 (Nudix hydrolase 24, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NUDT24 PE=2 SV=1)

HSP 1 Score: 402.1 bits (1032), Expect = 6.7e-111
Identity = 200/370 (54.05%), Postives = 262/370 (70.81%), Query Frame = 0

Query: 6   LAHSFRNFSTVPSFCIASTIFRTTY---TTISTFRPTVHRSISVASRPVSVTPGSFTWDD 65
           +A +F +    P+   +S     T    ++ S+  P +H S  ++   V ++P SFTW+D
Sbjct: 1   MASAFCSLCPTPTSLFSSHALIPTLQWRSSSSSRSPPLHISRVLSVETVPLSP-SFTWND 60

Query: 66  VVHVSSPESFQDDPADLTGYFEKVKLCNRGSDTQSEFFPFVIDGQIVGYVHHGFAKHLQQ 125
           V   S  E    + +DLTG+ EKV  CNRG +   EF PFVI+ QIVGY+H GF K+L+ 
Sbjct: 61  VFENSRKEYVPQNSSDLTGFLEKVDRCNRGLEKLGEFIPFVIEEQIVGYIHKGFTKYLRD 120

Query: 126 YPNVFTFSQDDSVKFGAYLTLHESLKTPEDRTRAVGDVVKCLGEE-VIPGTRNELFPVTS 185
           + ++FTFSQ     +G ++TL+  L  PE+RTRAV  V+K LG + +IPG RNEL+PV  
Sbjct: 121 FNDIFTFSQ-----YGGHVTLNMMLDKPEERTRAVAHVIKILGNKGIIPGIRNELYPVKP 180

Query: 186 SFGAPNFFSLERAAAPYFGIKVYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLVA 245
           SF AP FFS+ERAAAPYFG+K Y +H+NGYVE++G+KFLW+ KRS  K T+PG LDHLVA
Sbjct: 181 SFNAPAFFSIERAAAPYFGLKGYAIHVNGYVERDGQKFLWIGKRSLAKSTYPGKLDHLVA 240

Query: 246 GGLPQGISCGENLMKECKEEAGIPRSISKEAIPVGAISYTDIKGYGYKRDVQFCYDLKLP 305
           GGLP GIS  ENL+KEC+EEAGI + ++  AI VG +SY DI  Y + RDV FCYDL+LP
Sbjct: 241 GGLPHGISVCENLVKECEEEAGISKVLADRAIAVGVVSYMDIDRYCFTRDVLFCYDLELP 300

Query: 306 ESFVPENQDGEVEGFMLLPVTNVANVIRRTQFFKPNCSLVIINFLFRHGYIKPESSGYLE 365
           + FVP NQDGEV+ F L+PV  VANV+R+T FFK +CSLVII+FLFRHG I+PES GYL+
Sbjct: 301 QDFVPTNQDGEVDSFRLIPVAQVANVVRKTSFFKDSCSLVIIDFLFRHGLIRPESPGYLD 360

Query: 366 LLQGLRSGTC 372
           L + LR+G C
Sbjct: 361 LYRRLRNGDC 364

BLAST of IVF0005443 vs. ExPASy Swiss-Prot
Match: P47173 (Uncharacterized protein YJR142W OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YJR142W PE=1 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 4.6e-35
Identity = 86/210 (40.95%), Postives = 123/210 (58.57%), Query Frame = 0

Query: 169 IPGTRNELFPVTSSFGAPNFFSLERAAAPYFGIKVYGVHMNGYV--EKEGKKFLWVAKRS 228
           + G RNE + V        +  +ERA A   GI  YG+H+NGYV   K  K   WV +RS
Sbjct: 123 VKGWRNEKYAVW--VNKKPYVLVERAVAGVLGIITYGIHINGYVLDPKSKKVQFWVPRRS 182

Query: 229 QTKPTFPGMLDHLVAGGLPQGISCGENLMKECKEEAGIPRSISKEAI-PVGAISYT---- 288
           +TK T+P MLD+++AGGL       E ++KE  EEA + +S+ ++ I   G++SY     
Sbjct: 183 KTKQTWPLMLDNIIAGGLGYPYGIYETVLKESMEEANLEKSVIEDNIKATGSVSYLYFTG 242

Query: 289 DIKGYGYKR-------DVQFCYDLKLPESFVPENQDGEVEGFMLLPVTNVANVIRRTQFF 348
           DI    + +       +VQ+ YDLKL E  +P+  DGEVE F L  +    N +R+ + F
Sbjct: 243 DISVTKFNKESDFIVGEVQYVYDLKLSEDIIPKPNDGEVESFNLFSLQETINALRKKE-F 302

Query: 349 KPNCSLVIINFLFRHGYIKPESS-GYLELL 364
           KPNC+LV+++FL RHGYI PE+   YLEL+
Sbjct: 303 KPNCALVMVDFLIRHGYITPENEPNYLELV 329

BLAST of IVF0005443 vs. ExPASy Swiss-Prot
Match: P41888 (Thiamine pyrophosphokinase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=tnr3 PE=4 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 2.6e-30
Identity = 93/271 (34.32%), Postives = 139/271 (51.29%), Query Frame = 0

Query: 101 PFVIDGQIVGYVHHGFAKHLQQYPNVFTFSQDDSVKFGAYLTLHESLKTPEDRTRAVGDV 160
           PF  +G  +GYV     + L +  N   F  +     G Y+ ++ S  T E RT  +  V
Sbjct: 34  PFRANGAAIGYVTPLVLEILIKADN---FKFNWVYVPGEYIEINAS--TFEKRTDILAKV 93

Query: 161 VKCLGEE----VIPGTRNELFPVTSSFGAPNFFSLERAAAPYFGIKVYGVHMNGYV--EK 220
           ++         +    RNEL+ V      P   ++ER     FG    GVH   Y+   K
Sbjct: 94  LEHWRHNNTFGIADQWRNELYTVYGKSKKP-VLAVERGGFWLFGFLSTGVHCTMYIPATK 153

Query: 221 EGKKFLWVAKRSQTKPTFPGMLDHLVAGGLPQGISCGENLMKECKEEAGIPRSISKEAIP 280
           E    +WV +RS TK T+P  LD+ VAGG+  G S    ++KE  EEA +  S S   IP
Sbjct: 154 EHPLRIWVPRRSPTKQTWPNYLDNSVAGGIAHGDSVIGTMIKEFSEEANLDVS-SMNLIP 213

Query: 281 VGAISYTDI-KGYGYKRDVQFCYDLKLPESFVPENQDGEVEGFMLLPVTNVANVIRRTQF 340
            G +SY  + K +  + ++Q+ +DL + +  +P   DGEV GF LLP+  V + +   + 
Sbjct: 214 CGTVSYIKMEKRHWIQPELQYVFDLPVDDLVIPRINDGEVAGFSLLPLNQVLHEL-ELKS 273

Query: 341 FKPNCSLVIINFLFRHGYIKPESSGYLELLQ 365
           FKPNC+LV+++FL RHG I P+   YL+ L+
Sbjct: 274 FKPNCALVLLDFLIRHGIITPQHPQYLQTLE 296

BLAST of IVF0005443 vs. ExPASy TrEMBL
Match: A0A1S3CJ09 (nudix hydrolase 20, chloroplastic-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103501537 PE=4 SV=1)

HSP 1 Score: 755.7 bits (1950), Expect = 8.8e-215
Identity = 367/372 (98.66%), Postives = 368/372 (98.92%), Query Frame = 0

Query: 1   MASKSLAHSFRNFSTVPSFCIASTIFRTTYTTISTFRPTVHRSISVASRPVSVTPGSFTW 60
           MASKSLAHSFRNFSTVPSFC ASTIFRTTYTTISTFRPTVHRSISVASRPVSVTPGSFTW
Sbjct: 1   MASKSLAHSFRNFSTVPSFCNASTIFRTTYTTISTFRPTVHRSISVASRPVSVTPGSFTW 60

Query: 61  DDVVHVSSPESFQDDPADLTGYFEKVKLCNRGSDTQSEFFPFVIDGQIVGYVHHGFAKHL 120
           DDVVHVSSPESFQDDPADLTGYFEKVKLCNRGSDTQSEFFPFVIDGQIVGYVHHGFAKHL
Sbjct: 61  DDVVHVSSPESFQDDPADLTGYFEKVKLCNRGSDTQSEFFPFVIDGQIVGYVHHGFAKHL 120

Query: 121 QQYPNVFTFSQDDSVKFGAYLTLHESLKTPEDRTRAVGDVVKCLGEEVIPGTRNELFPVT 180
           QQYPNVFTFSQDDSVK GA LTLHESLKTPEDRTRAVGDVVKCLGEEVIPGTRNELFPVT
Sbjct: 121 QQYPNVFTFSQDDSVKVGACLTLHESLKTPEDRTRAVGDVVKCLGEEVIPGTRNELFPVT 180

Query: 181 SSFGAPNFFSLERAAAPYFGIKVYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLV 240
           SSFGAPNFFSLERAAAPYFGIKVYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLV
Sbjct: 181 SSFGAPNFFSLERAAAPYFGIKVYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLV 240

Query: 241 AGGLPQGISCGENLMKECKEEAGIPRSISKEAIPVGAISYTDIKGYGYKRDVQFCYDLKL 300
           AGGLPQGISCGENLMKECKEEAGIPRS+SKEAIPVGAISYTDIKGYGYKRDVQFCYDLKL
Sbjct: 241 AGGLPQGISCGENLMKECKEEAGIPRSVSKEAIPVGAISYTDIKGYGYKRDVQFCYDLKL 300

Query: 301 PESFVPENQDGEVEGFMLLPVTNVANVIRRTQFFKPNCSLVIINFLFRHGYIKPESSGYL 360
           PESFVPENQDGEVEGFMLLPVTNVANVIRRTQFFKPNCSLVIINFLFRHGYIKPESSGYL
Sbjct: 301 PESFVPENQDGEVEGFMLLPVTNVANVIRRTQFFKPNCSLVIINFLFRHGYIKPESSGYL 360

Query: 361 ELLQGLRSGTCF 373
           ELLQ LRSGTCF
Sbjct: 361 ELLQSLRSGTCF 372

BLAST of IVF0005443 vs. ExPASy TrEMBL
Match: A0A5A7TT66 (Nudix hydrolase 20 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold114G001740 PE=4 SV=1)

HSP 1 Score: 742.7 bits (1916), Expect = 7.7e-211
Identity = 367/395 (92.91%), Postives = 368/395 (93.16%), Query Frame = 0

Query: 1   MASKSLAHSFRNFSTVPSFCIASTIFRTTYTTISTFRPTVHRSISVASRPVSVTPGSFTW 60
           MASKSLAHSFRNFSTVPSFC ASTIFRTTYTTISTFRPTVHRSISVASRPVSVTPGSFTW
Sbjct: 1   MASKSLAHSFRNFSTVPSFCNASTIFRTTYTTISTFRPTVHRSISVASRPVSVTPGSFTW 60

Query: 61  DDVVHVSSPESFQDDPADLTGYFEKVKLCNRG-----------------------SDTQS 120
           DDVVHVSSPESFQDDPADLTGYFEKVKLCNRG                       SDTQS
Sbjct: 61  DDVVHVSSPESFQDDPADLTGYFEKVKLCNRGSVGLFLLFFSCFAVQTKDVKAFLSDTQS 120

Query: 121 EFFPFVIDGQIVGYVHHGFAKHLQQYPNVFTFSQDDSVKFGAYLTLHESLKTPEDRTRAV 180
           EFFPFVIDGQIVGYVHHGFAKHLQQYPNVFTFSQDDSVK GA LTLHESLKTPEDRTRAV
Sbjct: 121 EFFPFVIDGQIVGYVHHGFAKHLQQYPNVFTFSQDDSVKVGACLTLHESLKTPEDRTRAV 180

Query: 181 GDVVKCLGEEVIPGTRNELFPVTSSFGAPNFFSLERAAAPYFGIKVYGVHMNGYVEKEGK 240
           GDVVKCLGEEVIPGTRNELFPVTSSFGAPNFFSLERAAAPYFGIKVYGVHMNGYVEKEGK
Sbjct: 181 GDVVKCLGEEVIPGTRNELFPVTSSFGAPNFFSLERAAAPYFGIKVYGVHMNGYVEKEGK 240

Query: 241 KFLWVAKRSQTKPTFPGMLDHLVAGGLPQGISCGENLMKECKEEAGIPRSISKEAIPVGA 300
           KFLWVAKRSQTKPTFPGMLDHLVAGGLPQGISCGENLMKECKEEAGIPRS+SKEAIPVGA
Sbjct: 241 KFLWVAKRSQTKPTFPGMLDHLVAGGLPQGISCGENLMKECKEEAGIPRSVSKEAIPVGA 300

Query: 301 ISYTDIKGYGYKRDVQFCYDLKLPESFVPENQDGEVEGFMLLPVTNVANVIRRTQFFKPN 360
           ISYTDIKGYGYKRDVQFCYDLKLPESFVPENQDGEVEGFMLLPVTNVANVIRRTQFFKPN
Sbjct: 301 ISYTDIKGYGYKRDVQFCYDLKLPESFVPENQDGEVEGFMLLPVTNVANVIRRTQFFKPN 360

Query: 361 CSLVIINFLFRHGYIKPESSGYLELLQGLRSGTCF 373
           CSLVIINFLFRHGYIKPESSGYLELLQ LRSGTCF
Sbjct: 361 CSLVIINFLFRHGYIKPESSGYLELLQSLRSGTCF 395

BLAST of IVF0005443 vs. ExPASy TrEMBL
Match: A0A0A0LWL2 (Nudix hydrolase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G386580 PE=4 SV=1)

HSP 1 Score: 714.1 bits (1842), Expect = 2.9e-202
Identity = 350/372 (94.09%), Postives = 356/372 (95.70%), Query Frame = 0

Query: 1   MASKSLAHSFRNFSTVPSFCIASTIFRTTYTTISTFRPTVHRSISVASRPVSVTPGSFTW 60
           MASKSLAHS RNFSTV SFC ASTI RTTYTTISTFRP VHRSISVASRPVSVT GS TW
Sbjct: 1   MASKSLAHSLRNFSTVSSFCNASTISRTTYTTISTFRPKVHRSISVASRPVSVTAGSLTW 60

Query: 61  DDVVHVSSPESFQDDPADLTGYFEKVKLCNRGSDTQSEFFPFVIDGQIVGYVHHGFAKHL 120
           DDVVHVS PESFQDDPADLTGYFEKVKLCNRGSDTQSEFFPFVIDGQIVGY+HHGF KHL
Sbjct: 61  DDVVHVSLPESFQDDPADLTGYFEKVKLCNRGSDTQSEFFPFVIDGQIVGYMHHGFVKHL 120

Query: 121 QQYPNVFTFSQDDSVKFGAYLTLHESLKTPEDRTRAVGDVVKCLGEEVIPGTRNELFPVT 180
           QQYP VFT  +DDSVKFGA+LTLHESLKTPEDRTRAVGDVVKCLGEEVIPGTRNELFPVT
Sbjct: 121 QQYPKVFT--RDDSVKFGAFLTLHESLKTPEDRTRAVGDVVKCLGEEVIPGTRNELFPVT 180

Query: 181 SSFGAPNFFSLERAAAPYFGIKVYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLV 240
           SSFGA +FFSLERAAAPYFGIKVYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLV
Sbjct: 181 SSFGALSFFSLERAAAPYFGIKVYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLV 240

Query: 241 AGGLPQGISCGENLMKECKEEAGIPRSISKEAIPVGAISYTDIKGYGYKRDVQFCYDLKL 300
           AGGLPQGI CGENLMKEC+EEAGIPRSISKEAIPVGAISYTDIKGYGYKRDVQFCYDLKL
Sbjct: 241 AGGLPQGIPCGENLMKECQEEAGIPRSISKEAIPVGAISYTDIKGYGYKRDVQFCYDLKL 300

Query: 301 PESFVPENQDGEVEGFMLLPVTNVANVIRRTQFFKPNCSLVIINFLFRHGYIKPESSGYL 360
           PESFVPENQDGEVEGFMLLPVTNVANVIRRTQFFKPNCSLVII+FLFRHGYIKPESSGYL
Sbjct: 301 PESFVPENQDGEVEGFMLLPVTNVANVIRRTQFFKPNCSLVIIDFLFRHGYIKPESSGYL 360

Query: 361 ELLQGLRSGTCF 373
           ELLQ LR GTCF
Sbjct: 361 ELLQSLRGGTCF 370

BLAST of IVF0005443 vs. ExPASy TrEMBL
Match: A0A1S3CJI7 (nudix hydrolase 20, chloroplastic-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103501537 PE=4 SV=1)

HSP 1 Score: 686.8 bits (1771), Expect = 5.0e-194
Identity = 340/372 (91.40%), Postives = 341/372 (91.67%), Query Frame = 0

Query: 1   MASKSLAHSFRNFSTVPSFCIASTIFRTTYTTISTFRPTVHRSISVASRPVSVTPGSFTW 60
           MASKSLAHSFRNFSTVPSFC ASTIFRTTYTTISTFRPTVHRSISVASRPVSVTPGSFTW
Sbjct: 1   MASKSLAHSFRNFSTVPSFCNASTIFRTTYTTISTFRPTVHRSISVASRPVSVTPGSFTW 60

Query: 61  DDVVHVSSPESFQDDPADLTGYFEKVKLCNRGSDTQSEFFPFVIDGQIVGYVHHGFAKHL 120
           DDVVHVSSPESFQDDPADLTGYFEKVKLCNRGSDTQSEFFPFVIDGQIVGYVHHGFAKHL
Sbjct: 61  DDVVHVSSPESFQDDPADLTGYFEKVKLCNRGSDTQSEFFPFVIDGQIVGYVHHGFAKHL 120

Query: 121 QQYPNVFTFSQDDSVKFGAYLTLHESLKTPEDRTRAVGDVVKCLGEEVIPGTRNELFPVT 180
           QQYPNVFTFSQDDSVK GA LTLHESLKTPEDRTRAVGDVVKCLGEEVIPGTRNE     
Sbjct: 121 QQYPNVFTFSQDDSVKVGACLTLHESLKTPEDRTRAVGDVVKCLGEEVIPGTRNE----- 180

Query: 181 SSFGAPNFFSLERAAAPYFGIKVYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLV 240
                                 VYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLV
Sbjct: 181 ----------------------VYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLV 240

Query: 241 AGGLPQGISCGENLMKECKEEAGIPRSISKEAIPVGAISYTDIKGYGYKRDVQFCYDLKL 300
           AGGLPQGISCGENLMKECKEEAGIPRS+SKEAIPVGAISYTDIKGYGYKRDVQFCYDLKL
Sbjct: 241 AGGLPQGISCGENLMKECKEEAGIPRSVSKEAIPVGAISYTDIKGYGYKRDVQFCYDLKL 300

Query: 301 PESFVPENQDGEVEGFMLLPVTNVANVIRRTQFFKPNCSLVIINFLFRHGYIKPESSGYL 360
           PESFVPENQDGEVEGFMLLPVTNVANVIRRTQFFKPNCSLVIINFLFRHGYIKPESSGYL
Sbjct: 301 PESFVPENQDGEVEGFMLLPVTNVANVIRRTQFFKPNCSLVIINFLFRHGYIKPESSGYL 345

Query: 361 ELLQGLRSGTCF 373
           ELLQ LRSGTCF
Sbjct: 361 ELLQSLRSGTCF 345

BLAST of IVF0005443 vs. ExPASy TrEMBL
Match: A0A5D3BJT0 (Nudix hydrolase 20 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold596G00320 PE=4 SV=1)

HSP 1 Score: 681.8 bits (1758), Expect = 1.6e-192
Identity = 344/398 (86.43%), Postives = 344/398 (86.43%), Query Frame = 0

Query: 1   MASKSLAHSFRNFSTVPSFCIASTIFRTTYTTISTFRPTVHRSISVASRPVSVTPGSFTW 60
           MASKSLAHSFRNFSTVPSFCIASTIFRTTYTTISTFRPTVHRSISVASRPVSVTPGSFTW
Sbjct: 1   MASKSLAHSFRNFSTVPSFCIASTIFRTTYTTISTFRPTVHRSISVASRPVSVTPGSFTW 60

Query: 61  DDVVHVSSPESFQDDPADLTGYFEKVKLCNRGS--------------------------D 120
           DDVVHVSSPESFQDDPADLTGYFEKVKLCNRGS                          D
Sbjct: 61  DDVVHVSSPESFQDDPADLTGYFEKVKLCNRGSVGLFLLFFSCFAVQTKDVKAFLSLPID 120

Query: 121 TQSEFFPFVIDGQIVGYVHHGFAKHLQQYPNVFTFSQDDSVKFGAYLTLHESLKTPEDRT 180
           TQSEFFPFVIDGQIVGYVHHGFAKHLQQYPNVFTFSQDDSVKFGAYLTLHESLKTPEDRT
Sbjct: 121 TQSEFFPFVIDGQIVGYVHHGFAKHLQQYPNVFTFSQDDSVKFGAYLTLHESLKTPEDRT 180

Query: 181 RAVGDVVKCLGEEVIPGTRNELFPVTSSFGAPNFFSLERAAAPYFGIKVYGVHMNGYVEK 240
           RAVGDVVKCLGEEVIPGTRNE                           VYGVHMNGYVEK
Sbjct: 181 RAVGDVVKCLGEEVIPGTRNE---------------------------VYGVHMNGYVEK 240

Query: 241 EGKKFLWVAKRSQTKPTFPGMLDHLVAGGLPQGISCGENLMKECKEEAGIPRSISKEAIP 300
           EGKKFLWVAKRSQTKPTFPGMLDHLVAGGLPQGISCGENLMKECKEEAGIPRSISKEAIP
Sbjct: 241 EGKKFLWVAKRSQTKPTFPGMLDHLVAGGLPQGISCGENLMKECKEEAGIPRSISKEAIP 300

Query: 301 VGAISYTDIKGYGYKRDVQFCYDLKLPESFVPENQDGEVEGFMLLPVTNVANVIRRTQFF 360
           VGAISYTDIKGYGYKRDVQFCYDLKLPESFVPENQDGEVEGFMLLPVTNVANVIRRTQFF
Sbjct: 301 VGAISYTDIKGYGYKRDVQFCYDLKLPESFVPENQDGEVEGFMLLPVTNVANVIRRTQFF 360

Query: 361 KPNCSLVIINFLFRHGYIKPESSGYLELLQGLRSGTCF 373
           KPNCSLVIINFLFRHGYIKPESSGYLELLQ LRSGTCF
Sbjct: 361 KPNCSLVIINFLFRHGYIKPESSGYLELLQSLRSGTCF 371

BLAST of IVF0005443 vs. NCBI nr
Match: XP_008463362.1 (PREDICTED: nudix hydrolase 20, chloroplastic-like isoform X1 [Cucumis melo])

HSP 1 Score: 748 bits (1930), Expect = 2.44e-272
Identity = 367/372 (98.66%), Postives = 368/372 (98.92%), Query Frame = 0

Query: 1   MASKSLAHSFRNFSTVPSFCIASTIFRTTYTTISTFRPTVHRSISVASRPVSVTPGSFTW 60
           MASKSLAHSFRNFSTVPSFC ASTIFRTTYTTISTFRPTVHRSISVASRPVSVTPGSFTW
Sbjct: 1   MASKSLAHSFRNFSTVPSFCNASTIFRTTYTTISTFRPTVHRSISVASRPVSVTPGSFTW 60

Query: 61  DDVVHVSSPESFQDDPADLTGYFEKVKLCNRGSDTQSEFFPFVIDGQIVGYVHHGFAKHL 120
           DDVVHVSSPESFQDDPADLTGYFEKVKLCNRGSDTQSEFFPFVIDGQIVGYVHHGFAKHL
Sbjct: 61  DDVVHVSSPESFQDDPADLTGYFEKVKLCNRGSDTQSEFFPFVIDGQIVGYVHHGFAKHL 120

Query: 121 QQYPNVFTFSQDDSVKFGAYLTLHESLKTPEDRTRAVGDVVKCLGEEVIPGTRNELFPVT 180
           QQYPNVFTFSQDDSVK GA LTLHESLKTPEDRTRAVGDVVKCLGEEVIPGTRNELFPVT
Sbjct: 121 QQYPNVFTFSQDDSVKVGACLTLHESLKTPEDRTRAVGDVVKCLGEEVIPGTRNELFPVT 180

Query: 181 SSFGAPNFFSLERAAAPYFGIKVYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLV 240
           SSFGAPNFFSLERAAAPYFGIKVYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLV
Sbjct: 181 SSFGAPNFFSLERAAAPYFGIKVYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLV 240

Query: 241 AGGLPQGISCGENLMKECKEEAGIPRSISKEAIPVGAISYTDIKGYGYKRDVQFCYDLKL 300
           AGGLPQGISCGENLMKECKEEAGIPRS+SKEAIPVGAISYTDIKGYGYKRDVQFCYDLKL
Sbjct: 241 AGGLPQGISCGENLMKECKEEAGIPRSVSKEAIPVGAISYTDIKGYGYKRDVQFCYDLKL 300

Query: 301 PESFVPENQDGEVEGFMLLPVTNVANVIRRTQFFKPNCSLVIINFLFRHGYIKPESSGYL 360
           PESFVPENQDGEVEGFMLLPVTNVANVIRRTQFFKPNCSLVIINFLFRHGYIKPESSGYL
Sbjct: 301 PESFVPENQDGEVEGFMLLPVTNVANVIRRTQFFKPNCSLVIINFLFRHGYIKPESSGYL 360

Query: 361 ELLQGLRSGTCF 372
           ELLQ LRSGTCF
Sbjct: 361 ELLQSLRSGTCF 372

BLAST of IVF0005443 vs. NCBI nr
Match: KAA0046622.1 (nudix hydrolase 20 [Cucumis melo var. makuwa])

HSP 1 Score: 734 bits (1896), Expect = 8.86e-267
Identity = 367/395 (92.91%), Postives = 368/395 (93.16%), Query Frame = 0

Query: 1   MASKSLAHSFRNFSTVPSFCIASTIFRTTYTTISTFRPTVHRSISVASRPVSVTPGSFTW 60
           MASKSLAHSFRNFSTVPSFC ASTIFRTTYTTISTFRPTVHRSISVASRPVSVTPGSFTW
Sbjct: 1   MASKSLAHSFRNFSTVPSFCNASTIFRTTYTTISTFRPTVHRSISVASRPVSVTPGSFTW 60

Query: 61  DDVVHVSSPESFQDDPADLTGYFEKVKLCNRGS-----------------------DTQS 120
           DDVVHVSSPESFQDDPADLTGYFEKVKLCNRGS                       DTQS
Sbjct: 61  DDVVHVSSPESFQDDPADLTGYFEKVKLCNRGSVGLFLLFFSCFAVQTKDVKAFLSDTQS 120

Query: 121 EFFPFVIDGQIVGYVHHGFAKHLQQYPNVFTFSQDDSVKFGAYLTLHESLKTPEDRTRAV 180
           EFFPFVIDGQIVGYVHHGFAKHLQQYPNVFTFSQDDSVK GA LTLHESLKTPEDRTRAV
Sbjct: 121 EFFPFVIDGQIVGYVHHGFAKHLQQYPNVFTFSQDDSVKVGACLTLHESLKTPEDRTRAV 180

Query: 181 GDVVKCLGEEVIPGTRNELFPVTSSFGAPNFFSLERAAAPYFGIKVYGVHMNGYVEKEGK 240
           GDVVKCLGEEVIPGTRNELFPVTSSFGAPNFFSLERAAAPYFGIKVYGVHMNGYVEKEGK
Sbjct: 181 GDVVKCLGEEVIPGTRNELFPVTSSFGAPNFFSLERAAAPYFGIKVYGVHMNGYVEKEGK 240

Query: 241 KFLWVAKRSQTKPTFPGMLDHLVAGGLPQGISCGENLMKECKEEAGIPRSISKEAIPVGA 300
           KFLWVAKRSQTKPTFPGMLDHLVAGGLPQGISCGENLMKECKEEAGIPRS+SKEAIPVGA
Sbjct: 241 KFLWVAKRSQTKPTFPGMLDHLVAGGLPQGISCGENLMKECKEEAGIPRSVSKEAIPVGA 300

Query: 301 ISYTDIKGYGYKRDVQFCYDLKLPESFVPENQDGEVEGFMLLPVTNVANVIRRTQFFKPN 360
           ISYTDIKGYGYKRDVQFCYDLKLPESFVPENQDGEVEGFMLLPVTNVANVIRRTQFFKPN
Sbjct: 301 ISYTDIKGYGYKRDVQFCYDLKLPESFVPENQDGEVEGFMLLPVTNVANVIRRTQFFKPN 360

Query: 361 CSLVIINFLFRHGYIKPESSGYLELLQGLRSGTCF 372
           CSLVIINFLFRHGYIKPESSGYLELLQ LRSGTCF
Sbjct: 361 CSLVIINFLFRHGYIKPESSGYLELLQSLRSGTCF 395

BLAST of IVF0005443 vs. NCBI nr
Match: XP_004148712.1 (nudix hydrolase 20, chloroplastic isoform X3 [Cucumis sativus] >KGN65374.1 hypothetical protein Csa_020063 [Cucumis sativus])

HSP 1 Score: 706 bits (1823), Expect = 4.63e-256
Identity = 350/372 (94.09%), Postives = 356/372 (95.70%), Query Frame = 0

Query: 1   MASKSLAHSFRNFSTVPSFCIASTIFRTTYTTISTFRPTVHRSISVASRPVSVTPGSFTW 60
           MASKSLAHS RNFSTV SFC ASTI RTTYTTISTFRP VHRSISVASRPVSVT GS TW
Sbjct: 1   MASKSLAHSLRNFSTVSSFCNASTISRTTYTTISTFRPKVHRSISVASRPVSVTAGSLTW 60

Query: 61  DDVVHVSSPESFQDDPADLTGYFEKVKLCNRGSDTQSEFFPFVIDGQIVGYVHHGFAKHL 120
           DDVVHVS PESFQDDPADLTGYFEKVKLCNRGSDTQSEFFPFVIDGQIVGY+HHGF KHL
Sbjct: 61  DDVVHVSLPESFQDDPADLTGYFEKVKLCNRGSDTQSEFFPFVIDGQIVGYMHHGFVKHL 120

Query: 121 QQYPNVFTFSQDDSVKFGAYLTLHESLKTPEDRTRAVGDVVKCLGEEVIPGTRNELFPVT 180
           QQYP VFT  +DDSVKFGA+LTLHESLKTPEDRTRAVGDVVKCLGEEVIPGTRNELFPVT
Sbjct: 121 QQYPKVFT--RDDSVKFGAFLTLHESLKTPEDRTRAVGDVVKCLGEEVIPGTRNELFPVT 180

Query: 181 SSFGAPNFFSLERAAAPYFGIKVYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLV 240
           SSFGA +FFSLERAAAPYFGIKVYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLV
Sbjct: 181 SSFGALSFFSLERAAAPYFGIKVYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLV 240

Query: 241 AGGLPQGISCGENLMKECKEEAGIPRSISKEAIPVGAISYTDIKGYGYKRDVQFCYDLKL 300
           AGGLPQGI CGENLMKEC+EEAGIPRSISKEAIPVGAISYTDIKGYGYKRDVQFCYDLKL
Sbjct: 241 AGGLPQGIPCGENLMKECQEEAGIPRSISKEAIPVGAISYTDIKGYGYKRDVQFCYDLKL 300

Query: 301 PESFVPENQDGEVEGFMLLPVTNVANVIRRTQFFKPNCSLVIINFLFRHGYIKPESSGYL 360
           PESFVPENQDGEVEGFMLLPVTNVANVIRRTQFFKPNCSLVII+FLFRHGYIKPESSGYL
Sbjct: 301 PESFVPENQDGEVEGFMLLPVTNVANVIRRTQFFKPNCSLVIIDFLFRHGYIKPESSGYL 360

Query: 361 ELLQGLRSGTCF 372
           ELLQ LR GTCF
Sbjct: 361 ELLQSLRGGTCF 370

BLAST of IVF0005443 vs. NCBI nr
Match: XP_038879265.1 (nudix hydrolase 20, chloroplastic-like isoform X3 [Benincasa hispida])

HSP 1 Score: 683 bits (1762), Expect = 1.02e-246
Identity = 337/373 (90.35%), Postives = 349/373 (93.57%), Query Frame = 0

Query: 1   MASKSLA-HSFRNFSTVPSFCIASTIFRTTYTTISTFRPTVHRSISVASRPVSVTPGSFT 60
           MA KS+A HSFRNFSTV SFC ASTI RTTY   STFRPT+  SISVASR VSV  GSFT
Sbjct: 1   MAYKSMATHSFRNFSTVSSFCNASTISRTTYPMTSTFRPTLLHSISVASRSVSVAAGSFT 60

Query: 61  WDDVVHVSSPESFQDDPADLTGYFEKVKLCNRGSDTQSEFFPFVIDGQIVGYVHHGFAKH 120
           WDDVVHVS PESFQDDP+DLTGYFEKVKLCNRGSDTQSE  PFVID QIVGYVHHGFAKH
Sbjct: 61  WDDVVHVSLPESFQDDPSDLTGYFEKVKLCNRGSDTQSEILPFVIDDQIVGYVHHGFAKH 120

Query: 121 LQQYPNVFTFSQDDSVKFGAYLTLHESLKTPEDRTRAVGDVVKCLGEEVIPGTRNELFPV 180
           L+QYPNVFTF +DDSVKFGAYLTLHESLKTP+DRT AVGDVVKCLGE+VIPGTRNEL+PV
Sbjct: 121 LKQYPNVFTFPRDDSVKFGAYLTLHESLKTPKDRTLAVGDVVKCLGEKVIPGTRNELYPV 180

Query: 181 TSSFGAPNFFSLERAAAPYFGIKVYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHL 240
           TSSFGAPNFFSLERAAAPYFGIKVYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHL
Sbjct: 181 TSSFGAPNFFSLERAAAPYFGIKVYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHL 240

Query: 241 VAGGLPQGISCGENLMKECKEEAGIPRSISKEAIPVGAISYTDIKGYGYKRDVQFCYDLK 300
           VAGGLPQGI CGENLMKEC+EEAGIPRSISKEAIPVGAISYTDIKGYGYKRDVQFCYDLK
Sbjct: 241 VAGGLPQGIPCGENLMKECEEEAGIPRSISKEAIPVGAISYTDIKGYGYKRDVQFCYDLK 300

Query: 301 LPESFVPENQDGEVEGFMLLPVTNVANVIRRTQFFKPNCSLVIINFLFRHGYIKPESSGY 360
           LPE F+PENQDGEVEGFMLLPV NVANVIRRTQ FKPNCSLVI++FLFRHGYIKPESSGY
Sbjct: 301 LPEDFIPENQDGEVEGFMLLPVANVANVIRRTQLFKPNCSLVIMDFLFRHGYIKPESSGY 360

Query: 361 LELLQGLRSGTCF 372
           LELLQ LRSGTCF
Sbjct: 361 LELLQSLRSGTCF 373

BLAST of IVF0005443 vs. NCBI nr
Match: XP_008463363.1 (PREDICTED: nudix hydrolase 20, chloroplastic-like isoform X2 [Cucumis melo])

HSP 1 Score: 679 bits (1752), Expect = 1.20e-245
Identity = 340/372 (91.40%), Postives = 341/372 (91.67%), Query Frame = 0

Query: 1   MASKSLAHSFRNFSTVPSFCIASTIFRTTYTTISTFRPTVHRSISVASRPVSVTPGSFTW 60
           MASKSLAHSFRNFSTVPSFC ASTIFRTTYTTISTFRPTVHRSISVASRPVSVTPGSFTW
Sbjct: 1   MASKSLAHSFRNFSTVPSFCNASTIFRTTYTTISTFRPTVHRSISVASRPVSVTPGSFTW 60

Query: 61  DDVVHVSSPESFQDDPADLTGYFEKVKLCNRGSDTQSEFFPFVIDGQIVGYVHHGFAKHL 120
           DDVVHVSSPESFQDDPADLTGYFEKVKLCNRGSDTQSEFFPFVIDGQIVGYVHHGFAKHL
Sbjct: 61  DDVVHVSSPESFQDDPADLTGYFEKVKLCNRGSDTQSEFFPFVIDGQIVGYVHHGFAKHL 120

Query: 121 QQYPNVFTFSQDDSVKFGAYLTLHESLKTPEDRTRAVGDVVKCLGEEVIPGTRNELFPVT 180
           QQYPNVFTFSQDDSVK GA LTLHESLKTPEDRTRAVGDVVKCLGEEVIPGTRNE     
Sbjct: 121 QQYPNVFTFSQDDSVKVGACLTLHESLKTPEDRTRAVGDVVKCLGEEVIPGTRNE----- 180

Query: 181 SSFGAPNFFSLERAAAPYFGIKVYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLV 240
                                 VYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLV
Sbjct: 181 ----------------------VYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLV 240

Query: 241 AGGLPQGISCGENLMKECKEEAGIPRSISKEAIPVGAISYTDIKGYGYKRDVQFCYDLKL 300
           AGGLPQGISCGENLMKECKEEAGIPRS+SKEAIPVGAISYTDIKGYGYKRDVQFCYDLKL
Sbjct: 241 AGGLPQGISCGENLMKECKEEAGIPRSVSKEAIPVGAISYTDIKGYGYKRDVQFCYDLKL 300

Query: 301 PESFVPENQDGEVEGFMLLPVTNVANVIRRTQFFKPNCSLVIINFLFRHGYIKPESSGYL 360
           PESFVPENQDGEVEGFMLLPVTNVANVIRRTQFFKPNCSLVIINFLFRHGYIKPESSGYL
Sbjct: 301 PESFVPENQDGEVEGFMLLPVTNVANVIRRTQFFKPNCSLVIINFLFRHGYIKPESSGYL 345

Query: 361 ELLQGLRSGTCF 372
           ELLQ LRSGTCF
Sbjct: 361 ELLQSLRSGTCF 345

BLAST of IVF0005443 vs. TAIR 10
Match: AT5G19460.1 (nudix hydrolase homolog 20 )

HSP 1 Score: 429.5 bits (1103), Expect = 2.8e-120
Identity = 212/350 (60.57%), Postives = 270/350 (77.14%), Query Frame = 0

Query: 26  FRTTYTTISTFRPTVHRSISVASR-PVSVTPGSFTWDDVVHVSSPESFQDDPADLTGYFE 85
           +R++  ++S  R +  R++S A+  P+S    SFTWDDV+     E    + +DLTG+FE
Sbjct: 32  WRSSSMSLSPLRHS--RALSAATTVPIS---SSFTWDDVIETGRAEY---NSSDLTGFFE 91

Query: 86  KVKLCNRGSDTQSEFFPFVIDGQIVGYVHHGFAKHLQQYPNVFTFSQDDSV--KFGAYLT 145
           K+  CNRGS+   EF PFVI+ QIVGY+H  F ++L+++ ++FTFSQ+ S   +   Y+T
Sbjct: 92  KINRCNRGSEKLGEFIPFVIEEQIVGYIHKRFTEYLREFHDIFTFSQNGSCPDRVDGYVT 151

Query: 146 LHESLKTPEDRTRAVGDVVKCLGEE-VIPGTRNELFPVTSSFGAPNFFSLERAAAPYFGI 205
           L+  L+ PEDRTRAV DV+K LG++ +IPG RNEL+PV  SF AP FFSLERAAAPYFGI
Sbjct: 152 LNLMLQKPEDRTRAVADVIKILGDKGIIPGIRNELYPVKPSFNAPVFFSLERAAAPYFGI 211

Query: 206 KVYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLVAGGLPQGISCGENLMKECKEE 265
           K YGVHMNGYVE++G+K LW+ KRS +K T+PGMLDHLVAGGLP GISCG NL+KEC+EE
Sbjct: 212 KGYGVHMNGYVERDGQKLLWIGKRSLSKSTYPGMLDHLVAGGLPHGISCGGNLVKECEEE 271

Query: 266 AGIPRSISKEAIPVGAISYTDIKGYGYKRDVQFCYDLKLPESFVPENQDGEVEGFMLLPV 325
           AGI R+I+  AI VGA+SY DI  Y +KRDV FCYDL+LPE FVP+NQDGEVE F L+PV
Sbjct: 272 AGISRAIADRAIAVGAVSYLDIDQYCFKRDVLFCYDLELPEDFVPKNQDGEVESFKLIPV 331

Query: 326 TNVANVIRRTQFFKPNCSLVIINFLFRHGYIKPESSGYLELLQGLRSGTC 372
             VA+VI++T FFK NCSLVII+FLFRHG+I+PESSGYL+L Q LR+  C
Sbjct: 332 AQVASVIKKTSFFKANCSLVIIDFLFRHGFIRPESSGYLDLYQRLRNRDC 373

BLAST of IVF0005443 vs. TAIR 10
Match: AT5G19470.1 (nudix hydrolase homolog 24 )

HSP 1 Score: 402.1 bits (1032), Expect = 4.8e-112
Identity = 200/370 (54.05%), Postives = 262/370 (70.81%), Query Frame = 0

Query: 6   LAHSFRNFSTVPSFCIASTIFRTTY---TTISTFRPTVHRSISVASRPVSVTPGSFTWDD 65
           +A +F +    P+   +S     T    ++ S+  P +H S  ++   V ++P SFTW+D
Sbjct: 1   MASAFCSLCPTPTSLFSSHALIPTLQWRSSSSSRSPPLHISRVLSVETVPLSP-SFTWND 60

Query: 66  VVHVSSPESFQDDPADLTGYFEKVKLCNRGSDTQSEFFPFVIDGQIVGYVHHGFAKHLQQ 125
           V   S  E    + +DLTG+ EKV  CNRG +   EF PFVI+ QIVGY+H GF K+L+ 
Sbjct: 61  VFENSRKEYVPQNSSDLTGFLEKVDRCNRGLEKLGEFIPFVIEEQIVGYIHKGFTKYLRD 120

Query: 126 YPNVFTFSQDDSVKFGAYLTLHESLKTPEDRTRAVGDVVKCLGEE-VIPGTRNELFPVTS 185
           + ++FTFSQ     +G ++TL+  L  PE+RTRAV  V+K LG + +IPG RNEL+PV  
Sbjct: 121 FNDIFTFSQ-----YGGHVTLNMMLDKPEERTRAVAHVIKILGNKGIIPGIRNELYPVKP 180

Query: 186 SFGAPNFFSLERAAAPYFGIKVYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLVA 245
           SF AP FFS+ERAAAPYFG+K Y +H+NGYVE++G+KFLW+ KRS  K T+PG LDHLVA
Sbjct: 181 SFNAPAFFSIERAAAPYFGLKGYAIHVNGYVERDGQKFLWIGKRSLAKSTYPGKLDHLVA 240

Query: 246 GGLPQGISCGENLMKECKEEAGIPRSISKEAIPVGAISYTDIKGYGYKRDVQFCYDLKLP 305
           GGLP GIS  ENL+KEC+EEAGI + ++  AI VG +SY DI  Y + RDV FCYDL+LP
Sbjct: 241 GGLPHGISVCENLVKECEEEAGISKVLADRAIAVGVVSYMDIDRYCFTRDVLFCYDLELP 300

Query: 306 ESFVPENQDGEVEGFMLLPVTNVANVIRRTQFFKPNCSLVIINFLFRHGYIKPESSGYLE 365
           + FVP NQDGEV+ F L+PV  VANV+R+T FFK +CSLVII+FLFRHG I+PES GYL+
Sbjct: 301 QDFVPTNQDGEVDSFRLIPVAQVANVVRKTSFFKDSCSLVIIDFLFRHGLIRPESPGYLD 360

Query: 366 LLQGLRSGTC 372
           L + LR+G C
Sbjct: 361 LYRRLRNGDC 364

BLAST of IVF0005443 vs. TAIR 10
Match: AT5G19470.2 (nudix hydrolase homolog 24 )

HSP 1 Score: 386.3 bits (991), Expect = 2.7e-107
Identity = 195/370 (52.70%), Postives = 257/370 (69.46%), Query Frame = 0

Query: 6   LAHSFRNFSTVPSFCIASTIFRTTY---TTISTFRPTVHRSISVASRPVSVTPGSFTWDD 65
           +A +F +    P+   +S     T    ++ S+  P +H S  ++   V ++P SFTW+D
Sbjct: 1   MASAFCSLCPTPTSLFSSHALIPTLQWRSSSSSRSPPLHISRVLSVETVPLSP-SFTWND 60

Query: 66  VVHVSSPESFQDDPADLTGYFEKVKLCNRGSDTQSEFFPFVIDGQIVGYVHHGFAKHLQQ 125
           V   S  E    + +DLTG+ EKV  CNRG +   EF PFVI+ QIVGY+H GF K+L+ 
Sbjct: 61  VFENSRKEYVPQNSSDLTGFLEKVDRCNRGLEKLGEFIPFVIEEQIVGYIHKGFTKYLRD 120

Query: 126 YPNVFTFSQDDSVKFGAYLTLHESLKTPEDRTRAVGDVVKCLGEE-VIPGTRNELFPVTS 185
           + ++FTFSQ     +G ++TL+  L  PE+RTRAV  V+K LG + +IPG RNEL+PV  
Sbjct: 121 FNDIFTFSQ-----YGGHVTLNMMLDKPEERTRAVAHVIKILGNKGIIPGIRNELYPVKP 180

Query: 186 SFGAPNFFSLERAAAPYFGIKVYGVHMNGYVEKEGKKFLWVAKRSQTKPTFPGMLDHLVA 245
           SF AP FFS+ERAAAPYFG+K Y +H+NGYVE++G+KFLW+ KRS  K T+PG LDHLVA
Sbjct: 181 SFNAPAFFSIERAAAPYFGLKGYAIHVNGYVERDGQKFLWIGKRSLAKSTYPGKLDHLVA 240

Query: 246 GGLPQGISCGENLMKECKEEAGIPRSISKEAIPVGAISYTDIKGYGYKRDVQFCYDLKLP 305
           GGL        NL+KEC+EEAGI + ++  AI VG +SY DI  Y + RDV FCYDL+LP
Sbjct: 241 GGL--------NLVKECEEEAGISKVLADRAIAVGVVSYMDIDRYCFTRDVLFCYDLELP 300

Query: 306 ESFVPENQDGEVEGFMLLPVTNVANVIRRTQFFKPNCSLVIINFLFRHGYIKPESSGYLE 365
           + FVP NQDGEV+ F L+PV  VANV+R+T FFK +CSLVII+FLFRHG I+PES GYL+
Sbjct: 301 QDFVPTNQDGEVDSFRLIPVAQVANVVRKTSFFKDSCSLVIIDFLFRHGLIRPESPGYLD 356

Query: 366 LLQGLRSGTC 372
           L + LR+G C
Sbjct: 361 LYRRLRNGDC 356

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8VXZ03.9e-11960.57Nudix hydrolase 20, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NUDT20 PE=2... [more]
P0C0266.7e-11154.05Nudix hydrolase 24, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NUDT24 PE=2... [more]
P471734.6e-3540.95Uncharacterized protein YJR142W OS=Saccharomyces cerevisiae (strain ATCC 204508 ... [more]
P418882.6e-3034.32Thiamine pyrophosphokinase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843... [more]
Match NameE-valueIdentityDescription
A0A1S3CJ098.8e-21598.66nudix hydrolase 20, chloroplastic-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC... [more]
A0A5A7TT667.7e-21192.91Nudix hydrolase 20 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold114G0... [more]
A0A0A0LWL22.9e-20294.09Nudix hydrolase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G38... [more]
A0A1S3CJI75.0e-19491.40nudix hydrolase 20, chloroplastic-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC... [more]
A0A5D3BJT01.6e-19286.43Nudix hydrolase 20 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold596G0... [more]
Match NameE-valueIdentityDescription
XP_008463362.12.44e-27298.66PREDICTED: nudix hydrolase 20, chloroplastic-like isoform X1 [Cucumis melo][more]
KAA0046622.18.86e-26792.91nudix hydrolase 20 [Cucumis melo var. makuwa][more]
XP_004148712.14.63e-25694.09nudix hydrolase 20, chloroplastic isoform X3 [Cucumis sativus] >KGN65374.1 hypot... [more]
XP_038879265.11.02e-24690.35nudix hydrolase 20, chloroplastic-like isoform X3 [Benincasa hispida][more]
XP_008463363.11.20e-24591.40PREDICTED: nudix hydrolase 20, chloroplastic-like isoform X2 [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT5G19460.12.8e-12060.57nudix hydrolase homolog 20 [more]
AT5G19470.14.8e-11254.05nudix hydrolase homolog 24 [more]
AT5G19470.22.7e-10752.70nudix hydrolase homolog 24 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.90.79.10Nucleoside Triphosphate Pyrophosphohydrolasecoord: 188..368
e-value: 1.7E-69
score: 235.2
NoneNo IPR availablePANTHERPTHR13622THIAMIN PYROPHOSPHOKINASEcoord: 58..369
NoneNo IPR availablePANTHERPTHR13622:SF10SI:DKEY-6N6.2coord: 58..369
NoneNo IPR availableCDDcd03676Nudix_hydrolase_3coord: 171..350
e-value: 1.47978E-80
score: 242.555
IPR031804Domain of unknown function DUF4743PFAMPF15916DUF4743coord: 82..202
e-value: 1.0E-36
score: 125.5
IPR000086NUDIX hydrolase domainPFAMPF00293NUDIXcoord: 215..324
e-value: 2.4E-6
score: 27.6
IPR000086NUDIX hydrolase domainPROSITEPS51462NUDIXcoord: 203..344
score: 11.430145
IPR015797NUDIX hydrolase-like domain superfamilySUPERFAMILY55811Nudixcoord: 183..351

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0005443.1IVF0005443.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0044715 8-oxo-dGDP phosphatase activity