IVF0000244 (gene) Melon (IVF77) v1

Overview
NameIVF0000244
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Descriptionprotein NUCLEAR FUSION DEFECTIVE 4
Locationchr03: 1576053 .. 1578641 (+)
RNA-Seq ExpressionIVF0000244
SyntenyIVF0000244
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTCGGTGGAATGACAAACTTGTTGCCTTTATCAACAACAGATGGTTGGTTTTTGTTGCTGCAATATGGCTTCAATCTTGGGCTGGAATTGGGTATCTTTTTGGAAGTATATCGCCGGTAATCAAAACCAATTTGAGTTACAATCAGAGACAAGTTTCTCGTCTTGGTGTCGCTAAAGATCTTGGCGATAGCGTTGGCTTTTTGGCTGCCACGTTAACGGAGATATTGCCCTTTTGGGGAAGTCTCTTGGTCGGTGCTATCCATAACATTGTTGGGTATGGTTGGGTTTGGCTCATCGTCACTGGTCGAGCTCCCGTTTTGCCTCTCTGGGCTGTAAGTTTTTTGCTCTTTATCTTTTAATGCTCTCTGTTTGGGTTTTGACCTTTGTCTTTAGATGTTTCTAATGAGATATCCATATTCAAATCTTGATTGAATGGCTTCTGATTGAGATATTTTGCTTTTGCGAATTAATTTCAAAAGTTACATAGTGATTAGCGGTCAACGAGTTGGAATCTGTTGTTGGCTTCAAAAGTGAATCTGGGGATGGGGAGATGATTCTTTCGTGGTTAGCCTTAATGAGGATTGTTAGATTAAAGTTGCTATTTGTCTTAGAATTTTCTCTGAATGGTTACTCTAAGTTGTGTTGTAGTAAAATGCGATTGGATGCAGATTAACTGTTTGATAATTTGTTTTCTACGACTATAGAAGCAATTTTATTGAATATTTCATGGATGCAGAACTGTTTGTTGTGGATAGTACTTTACAAACAGATCTGCTGAATATATAGTTTGGTTGAGGCTATTGAAAAGTCCTTTTGGAAGTTCCAGAATCAATCCTTCTCAACCTGTTCATTAAGATTGGTAAAATGCATCATCTTCTAGCCTTTTTACTCTAGAATTGAAGGAAATGAGGGGTGAGATGCACGAATGGCAGTGACCAAACCACTCTAACCAATTAAAGTTTGAAGAACAAATTGTTCAGCTATTTTCTTCTTTTACCATTTTTGTTTAGTTAAGTATGGATATCATTGCGCAGATGTGCGTTCTTGTATTTGTGGGAACGAACGGGGAGACGTACTTCAATACAGTTTCTTTGGTTTCTTGTGTACAAAACTTTCCGAAAAGCAGAGGCCCTGTGGTCGGGATTCTGAAAGGCTTTGCTGGTTTAAGTGGTGCAATTTTGACTCAGATATATGCAATCATCCATTCTCCTGATTCAGCCAATCTTATATTCATGGTTGCAGTAGGTCCTGCACTGGTGGCTATTGGTGTGATGTTTTTTATCAGACCTGTTGCCGGTCATCGACAAGTAAGGCCGTCCGATGGCATGAGCTTTTCTAGTGTTTATGGTGTCTGCCTCCTTTTGGCTGCATATTTGATGGGAGTCATGCTTATTGAGGATCTTGTTACATTGAGCCCCACTGTGATAACCATATTTACTGTGGTTATGTTTGTCATCCTTCTTACGCCCTTTTTAATTCCCGTGACATTGACTTTTAGCTCGGAGACGACAACATATGCTGAGCAAGAGGCTCTCCTACCACCCTCTGAGAAAGAGGAACCTGCTAGAACTGAACCAGATGGTAATGAAGTGATATTTAGTGAGGTGGAAGATGAAAAATCCGAGGGAGAGGACTTACTTCCTGCATCAGAGAGACAAAAACGCATTGCTCAGTTGCAAGCAAAACTATTGCAAGCAGCCGCAGAAGGAGCAGTAAGAGTCAAGAGGAGAAAGGGTCCACGCCGAGGGGAAGACTTCACTTTGGGTCAAGCTTTAATCAAAGCAGACTTTTGGCTTATATTTTCCTCCCATCTTCTTGGTTCCGGAACTGGCTTGACCGTGATTGATAACCTCGGACAGATGAGCCAGTCTTTAGGTTACGATAATACACATATTTTTGTATCCCTGATCAGCATATGGAACTTTCTCGGCCGTGTTGGTGGTGGATATCTCTCTGAGATTGTTGTGAGGTACAAAAGTTTGTTTTAGTTGTCTCAATGCCAGTAAATAAAACTTTTTACTTCTTAAAATGTTCCATTGTATCTTACTATCATTTTTTTCAACAGAGATTTTGCTTATCCAAGACCCATTGCAATGACGATTGCGCAAGTCCTTATGATATTTGGACATGTCTTCATCGGTATGGGATGGCCCGGGGCAATGTACATTGGCACTCTCATAACAGGGCTCGGTTACGGGGCTCACTGGGCCATTGTACCTGCTACTGCCTCTGAATTGTTTGGCTTGAAAAAGTTTGGCGCTTTATACAACTTCATCACTCTCTCAACTCCTATGGGATCTTTGATATTTTCTGGCTTAATTGCGAGCAGTATATATGACAGCGAAGCCGAGAAGCAAGCTCGTAATCATCTCACACAGTTCCAGAGCTCCTCGTCATTGTGGTTCACTAGACTTTACGCCGAGGGACCACACAAGTGCGAGGGTGCCATATGCTTCTTTTTAACTTGTATGATAATGGCTGGATTCTGCGCGATTGCAGGGATATTAAGTTTAATTTTGGTGTATCGGACAAAGGGTGTTTACCACAACCTCTATGGAAAATCTCGTACATCAACACTTTCGTAG

mRNA sequence

ATGGGTCGGTGGAATGACAAACTTGTTGCCTTTATCAACAACAGATGGTTGGTTTTTGTTGCTGCAATATGGCTTCAATCTTGGGCTGGAATTGGGTATCTTTTTGGAAGTATATCGCCGGTAATCAAAACCAATTTGAGTTACAATCAGAGACAAGTTTCTCGTCTTGGTGTCGCTAAAGATCTTGGCGATAGCGTTGGCTTTTTGGCTGCCACGTTAACGGAGATATTGCCCTTTTGGGGAAGTCTCTTGGTCGGTGCTATCCATAACATTGTTGGGTATGGTTGGGTTTGGCTCATCGTCACTGGTCGAGCTCCCGTTTTGCCTCTCTGGGCTATGTGCGTTCTTGTATTTGTGGGAACGAACGGGGAGACGTACTTCAATACAGTTTCTTTGGTTTCTTGTGTACAAAACTTTCCGAAAAGCAGAGGCCCTGTGGTCGGGATTCTGAAAGGCTTTGCTGGTTTAAGTGGTGCAATTTTGACTCAGATATATGCAATCATCCATTCTCCTGATTCAGCCAATCTTATATTCATGGTTGCAGTAGGTCCTGCACTGGTGGCTATTGGTGTGATGTTTTTTATCAGACCTGTTGCCGGTCATCGACAAGTAAGGCCGTCCGATGGCATGAGCTTTTCTAGTGTTTATGGTGTCTGCCTCCTTTTGGCTGCATATTTGATGGGAGTCATGCTTATTGAGGATCTTGTTACATTGAGCCCCACTGTGATAACCATATTTACTGTGGTTATGTTTGTCATCCTTCTTACGCCCTTTTTAATTCCCGTGACATTGACTTTTAGCTCGGAGACGACAACATATGCTGAGCAAGAGGCTCTCCTACCACCCTCTGAGAAAGAGGAACCTGCTAGAACTGAACCAGATGGTAATGAAGTGATATTTAGTGAGGTGGAAGATGAAAAATCCGAGGGAGAGGACTTACTTCCTGCATCAGAGAGACAAAAACGCATTGCTCAGTTGCAAGCAAAACTATTGCAAGCAGCCGCAGAAGGAGCAGTAAGAGTCAAGAGGAGAAAGGGTCCACGCCGAGGGGAAGACTTCACTTTGGGTCAAGCTTTAATCAAAGCAGACTTTTGGCTTATATTTTCCTCCCATCTTCTTGGTTCCGGAACTGGCTTGACCGTGATTGATAACCTCGGACAGATGAGCCAGTCTTTAGGTTACGATAATACACATATTTTTGTATCCCTGATCAGCATATGGAACTTTCTCGGCCGTGTTGGTGGTGGATATCTCTCTGAGATTGTTGTGAGAGATTTTGCTTATCCAAGACCCATTGCAATGACGATTGCGCAAGTCCTTATGATATTTGGACATGTCTTCATCGGTATGGGATGGCCCGGGGCAATGTACATTGGCACTCTCATAACAGGGCTCGGTTACGGGGCTCACTGGGCCATTGTACCTGCTACTGCCTCTGAATTGTTTGGCTTGAAAAAGTTTGGCGCTTTATACAACTTCATCACTCTCTCAACTCCTATGGGATCTTTGATATTTTCTGGCTTAATTGCGAGCAGTATATATGACAGCGAAGCCGAGAAGCAAGCTCGTAATCATCTCACACAGTTCCAGAGCTCCTCGTCATTGTGGTTCACTAGACTTTACGCCGAGGGACCACACAAGTGCGAGGGTGCCATATGCTTCTTTTTAACTTGTATGATAATGGCTGGATTCTGCGCGATTGCAGGGATATTAAGTTTAATTTTGGTGTATCGGACAAAGGGTGTTTACCACAACCTCTATGGAAAATCTCGTACATCAACACTTTCGTAG

Coding sequence (CDS)

ATGGGTCGGTGGAATGACAAACTTGTTGCCTTTATCAACAACAGATGGTTGGTTTTTGTTGCTGCAATATGGCTTCAATCTTGGGCTGGAATTGGGTATCTTTTTGGAAGTATATCGCCGGTAATCAAAACCAATTTGAGTTACAATCAGAGACAAGTTTCTCGTCTTGGTGTCGCTAAAGATCTTGGCGATAGCGTTGGCTTTTTGGCTGCCACGTTAACGGAGATATTGCCCTTTTGGGGAAGTCTCTTGGTCGGTGCTATCCATAACATTGTTGGGTATGGTTGGGTTTGGCTCATCGTCACTGGTCGAGCTCCCGTTTTGCCTCTCTGGGCTATGTGCGTTCTTGTATTTGTGGGAACGAACGGGGAGACGTACTTCAATACAGTTTCTTTGGTTTCTTGTGTACAAAACTTTCCGAAAAGCAGAGGCCCTGTGGTCGGGATTCTGAAAGGCTTTGCTGGTTTAAGTGGTGCAATTTTGACTCAGATATATGCAATCATCCATTCTCCTGATTCAGCCAATCTTATATTCATGGTTGCAGTAGGTCCTGCACTGGTGGCTATTGGTGTGATGTTTTTTATCAGACCTGTTGCCGGTCATCGACAAGTAAGGCCGTCCGATGGCATGAGCTTTTCTAGTGTTTATGGTGTCTGCCTCCTTTTGGCTGCATATTTGATGGGAGTCATGCTTATTGAGGATCTTGTTACATTGAGCCCCACTGTGATAACCATATTTACTGTGGTTATGTTTGTCATCCTTCTTACGCCCTTTTTAATTCCCGTGACATTGACTTTTAGCTCGGAGACGACAACATATGCTGAGCAAGAGGCTCTCCTACCACCCTCTGAGAAAGAGGAACCTGCTAGAACTGAACCAGATGGTAATGAAGTGATATTTAGTGAGGTGGAAGATGAAAAATCCGAGGGAGAGGACTTACTTCCTGCATCAGAGAGACAAAAACGCATTGCTCAGTTGCAAGCAAAACTATTGCAAGCAGCCGCAGAAGGAGCAGTAAGAGTCAAGAGGAGAAAGGGTCCACGCCGAGGGGAAGACTTCACTTTGGGTCAAGCTTTAATCAAAGCAGACTTTTGGCTTATATTTTCCTCCCATCTTCTTGGTTCCGGAACTGGCTTGACCGTGATTGATAACCTCGGACAGATGAGCCAGTCTTTAGGTTACGATAATACACATATTTTTGTATCCCTGATCAGCATATGGAACTTTCTCGGCCGTGTTGGTGGTGGATATCTCTCTGAGATTGTTGTGAGAGATTTTGCTTATCCAAGACCCATTGCAATGACGATTGCGCAAGTCCTTATGATATTTGGACATGTCTTCATCGGTATGGGATGGCCCGGGGCAATGTACATTGGCACTCTCATAACAGGGCTCGGTTACGGGGCTCACTGGGCCATTGTACCTGCTACTGCCTCTGAATTGTTTGGCTTGAAAAAGTTTGGCGCTTTATACAACTTCATCACTCTCTCAACTCCTATGGGATCTTTGATATTTTCTGGCTTAATTGCGAGCAGTATATATGACAGCGAAGCCGAGAAGCAAGCTCGTAATCATCTCACACAGTTCCAGAGCTCCTCGTCATTGTGGTTCACTAGACTTTACGCCGAGGGACCACACAAGTGCGAGGGTGCCATATGCTTCTTTTTAACTTGTATGATAATGGCTGGATTCTGCGCGATTGCAGGGATATTAAGTTTAATTTTGGTGTATCGGACAAAGGGTGTTTACCACAACCTCTATGGAAAATCTCGTACATCAACACTTTCGTAG

Protein sequence

MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPVIKTNLSYNQRQVSRLGVAKDLGDSVGFLAATLTEILPFWGSLLVGAIHNIVGYGWVWLIVTGRAPVLPLWAMCVLVFVGTNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIIHSPDSANLIFMVAVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLIEDLVTLSPTVITIFTVVMFVILLTPFLIPVTLTFSSETTTYAEQEALLPPSEKEEPARTEPDGNEVIFSEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALIKADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSEIVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASELFGLKKFGALYNFITLSTPMGSLIFSGLIASSIYDSEAEKQARNHLTQFQSSSSLWFTRLYAEGPHKCEGAICFFLTCMIMAGFCAIAGILSLILVYRTKGVYHNLYGKSRTSTLS
Homology
BLAST of IVF0000244 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 2.4e-30
Identity = 153/593 (25.80%), Postives = 264/593 (44.52%), Query Frame = 0

Query: 2   GRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPVIKTNLSYNQRQVSRLGVAKD 61
           GRW          +W V VAAIW+Q+  G  + F + S  +K+ L  +Q +++ L VA D
Sbjct: 39  GRW---------RKWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASD 98

Query: 62  LGDSVGFLAATLTEILPFWGSLLVGAIHNIVGYGWVWLIVTG--RAPVLPLWAMCVLVFV 121
           LG + G+ +       P    L   A    VGYG  WL++T     P   ++  C+L  +
Sbjct: 99  LGKAFGWSSGIALGYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLPYSLVFLCCLLAGL 158

Query: 122 GTNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIIHSPDSANLIFM 181
                 +FNT   + C+++FP +R   + +   F G+S A+ +  +  I +P S+NL  +
Sbjct: 159 SI---CWFNTACFILCIRHFPNNRALALSLTVSFNGISAALYSLAFNAI-NPSSSNLYLL 218

Query: 182 V-AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFSS-VYGVCLLLAAYLMGVMLIEDLVT 241
           + ++ P +V+   ++ +            D     S V+ +  +LA      +L+    T
Sbjct: 219 LNSLVPLVVSFAALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSST 278

Query: 242 LSPTVITIFTVVMFVI-LLTPFLIPVTLTFSSETTTYAEQEALLPPSEKEEPARTEPDGN 301
            S  +  I  VV+ V  L  P L+            YA ++  LP               
Sbjct: 279 SSARLNFIGAVVLLVFPLCAPLLV------------YA-RDYFLP--------------- 338

Query: 302 EVIFSEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLG 361
            VI + +  E S G  +L   E + +   + +K      E     K     R G++ +  
Sbjct: 339 -VINARLNHE-SSGYVMLNIDELKNQKTSVSSK---TGYEHMGTAKEGNTVRLGDEHSFR 398

Query: 362 QALIKADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGG 421
             + + +FWL + ++  G   GL   +NLGQ++QSLG ++T + V++ S ++F GR+   
Sbjct: 399 LLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLGQNSTTL-VTIYSSFSFFGRLLSA 458

Query: 422 YLSEIVVRDFAYPRP--IAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIV 481
              + + + F   R    A+ +    + F  + +      A+   T + GL  G  +A  
Sbjct: 459 -APDFMHKRFRLTRTGWFAIALLPTPIAFFLLAVSSSQQTALQTATALIGLSSGFIFAAA 518

Query: 482 PATASELFGLKKFGALYNFITLSTPMGSLIFSGLIASSIYDSEAEKQARNHLTQFQSSSS 541
            +  S+LFG    G  +N +  + P+GSL++ G IA+SIY++ A       +T   S S 
Sbjct: 519 VSITSDLFGPNSVGVNHNILITNIPIGSLLY-GYIAASIYEANASPD----ITPIVSDSI 565

Query: 542 LWFTRLYAEGPHKCEGAICFFLTCMIMAGFCAIAGILSLILVY-RTKGVYHNL 587
           +            C G  C+F T  +  G  +I G++S + +Y RTK VYH L
Sbjct: 579 V------------CIGRDCYFKT-FVFWGCLSILGVVSSLSLYIRTKPVYHRL 565

BLAST of IVF0000244 vs. ExPASy TrEMBL
Match: A0A5A7UN83 (Protein NUCLEAR FUSION DEFECTIVE 4 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1154G00120 PE=4 SV=1)

HSP 1 Score: 1157.5 bits (2993), Expect = 0.0e+00
Identity = 596/596 (100.00%), Postives = 596/596 (100.00%), Query Frame = 0

Query: 1   MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPVIKTNLSYNQRQVSRLGVAK 60
           MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPVIKTNLSYNQRQVSRLGVAK
Sbjct: 1   MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPVIKTNLSYNQRQVSRLGVAK 60

Query: 61  DLGDSVGFLAATLTEILPFWGSLLVGAIHNIVGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120
           DLGDSVGFLAATLTEILPFWGSLLVGAIHNIVGYGWVWLIVTGRAPVLPLWAMCVLVFVG
Sbjct: 61  DLGDSVGFLAATLTEILPFWGSLLVGAIHNIVGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120

Query: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIIHSPDSANLIFMV 180
           TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIIHSPDSANLIFMV
Sbjct: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIIHSPDSANLIFMV 180

Query: 181 AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLIEDLVTLSP 240
           AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLIEDLVTLSP
Sbjct: 181 AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLIEDLVTLSP 240

Query: 241 TVITIFTVVMFVILLTPFLIPVTLTFSSETTTYAEQEALLPPSEKEEPARTEPDGNEVIF 300
           TVITIFTVVMFVILLTPFLIPVTLTFSSETTTYAEQEALLPPSEKEEPARTEPDGNEVIF
Sbjct: 241 TVITIFTVVMFVILLTPFLIPVTLTFSSETTTYAEQEALLPPSEKEEPARTEPDGNEVIF 300

Query: 301 SEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360
           SEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI
Sbjct: 301 SEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360

Query: 361 KADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSE 420
           KADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSE
Sbjct: 361 KADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSE 420

Query: 421 IVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480
           IVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE
Sbjct: 421 IVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480

Query: 481 LFGLKKFGALYNFITLSTPMGSLIFSGLIASSIYDSEAEKQARNHLTQFQSSSSLWFTRL 540
           LFGLKKFGALYNFITLSTPMGSLIFSGLIASSIYDSEAEKQARNHLTQFQSSSSLWFTRL
Sbjct: 481 LFGLKKFGALYNFITLSTPMGSLIFSGLIASSIYDSEAEKQARNHLTQFQSSSSLWFTRL 540

Query: 541 YAEGPHKCEGAICFFLTCMIMAGFCAIAGILSLILVYRTKGVYHNLYGKSRTSTLS 597
           YAEGPHKCEGAICFFLTCMIMAGFCAIAGILSLILVYRTKGVYHNLYGKSRTSTLS
Sbjct: 541 YAEGPHKCEGAICFFLTCMIMAGFCAIAGILSLILVYRTKGVYHNLYGKSRTSTLS 596

BLAST of IVF0000244 vs. ExPASy TrEMBL
Match: A0A1S3B357 (protein NUCLEAR FUSION DEFECTIVE 4 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103485475 PE=4 SV=1)

HSP 1 Score: 1157.5 bits (2993), Expect = 0.0e+00
Identity = 596/596 (100.00%), Postives = 596/596 (100.00%), Query Frame = 0

Query: 1   MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPVIKTNLSYNQRQVSRLGVAK 60
           MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPVIKTNLSYNQRQVSRLGVAK
Sbjct: 1   MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPVIKTNLSYNQRQVSRLGVAK 60

Query: 61  DLGDSVGFLAATLTEILPFWGSLLVGAIHNIVGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120
           DLGDSVGFLAATLTEILPFWGSLLVGAIHNIVGYGWVWLIVTGRAPVLPLWAMCVLVFVG
Sbjct: 61  DLGDSVGFLAATLTEILPFWGSLLVGAIHNIVGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120

Query: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIIHSPDSANLIFMV 180
           TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIIHSPDSANLIFMV
Sbjct: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIIHSPDSANLIFMV 180

Query: 181 AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLIEDLVTLSP 240
           AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLIEDLVTLSP
Sbjct: 181 AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLIEDLVTLSP 240

Query: 241 TVITIFTVVMFVILLTPFLIPVTLTFSSETTTYAEQEALLPPSEKEEPARTEPDGNEVIF 300
           TVITIFTVVMFVILLTPFLIPVTLTFSSETTTYAEQEALLPPSEKEEPARTEPDGNEVIF
Sbjct: 241 TVITIFTVVMFVILLTPFLIPVTLTFSSETTTYAEQEALLPPSEKEEPARTEPDGNEVIF 300

Query: 301 SEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360
           SEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI
Sbjct: 301 SEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360

Query: 361 KADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSE 420
           KADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSE
Sbjct: 361 KADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSE 420

Query: 421 IVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480
           IVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE
Sbjct: 421 IVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480

Query: 481 LFGLKKFGALYNFITLSTPMGSLIFSGLIASSIYDSEAEKQARNHLTQFQSSSSLWFTRL 540
           LFGLKKFGALYNFITLSTPMGSLIFSGLIASSIYDSEAEKQARNHLTQFQSSSSLWFTRL
Sbjct: 481 LFGLKKFGALYNFITLSTPMGSLIFSGLIASSIYDSEAEKQARNHLTQFQSSSSLWFTRL 540

Query: 541 YAEGPHKCEGAICFFLTCMIMAGFCAIAGILSLILVYRTKGVYHNLYGKSRTSTLS 597
           YAEGPHKCEGAICFFLTCMIMAGFCAIAGILSLILVYRTKGVYHNLYGKSRTSTLS
Sbjct: 541 YAEGPHKCEGAICFFLTCMIMAGFCAIAGILSLILVYRTKGVYHNLYGKSRTSTLS 596

BLAST of IVF0000244 vs. ExPASy TrEMBL
Match: A0A0A0LQ60 (Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G406130 PE=4 SV=1)

HSP 1 Score: 1128.2 bits (2917), Expect = 0.0e+00
Identity = 576/596 (96.64%), Postives = 584/596 (97.99%), Query Frame = 0

Query: 1   MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPVIKTNLSYNQRQVSRLGVAK 60
           MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISP+IKTNLSYNQRQ+SRLGVAK
Sbjct: 1   MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPIIKTNLSYNQRQISRLGVAK 60

Query: 61  DLGDSVGFLAATLTEILPFWGSLLVGAIHNIVGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120
           DLGDSVGFLAATLTEILPFWGSLLVGAIHN VGYGWVWLIVTGRAPVLPLWAMC LVF+G
Sbjct: 61  DLGDSVGFLAATLTEILPFWGSLLVGAIHNFVGYGWVWLIVTGRAPVLPLWAMCALVFIG 120

Query: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIIHSPDSANLIFMV 180
           TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQ YAI HSP+SANLIFMV
Sbjct: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQTYAIFHSPESANLIFMV 180

Query: 181 AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLIEDLVTLSP 240
           AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSF+SVYGVCLLLAAYLMGVMLIEDLVTLSP
Sbjct: 181 AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFTSVYGVCLLLAAYLMGVMLIEDLVTLSP 240

Query: 241 TVITIFTVVMFVILLTPFLIPVTLTFSSETTTYAEQEALLPPSEKEEPARTEPDGNEVIF 300
            VITIFTVVMFVILLTPF IPV+LT SSE TTYAEQEALLPPSEKEEPARTEPDGNEVIF
Sbjct: 241 IVITIFTVVMFVILLTPFFIPVSLTLSSEATTYAEQEALLPPSEKEEPARTEPDGNEVIF 300

Query: 301 SEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360
           SEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI
Sbjct: 301 SEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360

Query: 361 KADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSE 420
           KADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSE
Sbjct: 361 KADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSE 420

Query: 421 IVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480
           IVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE
Sbjct: 421 IVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480

Query: 481 LFGLKKFGALYNFITLSTPMGSLIFSGLIASSIYDSEAEKQARNHLTQFQSSSSLWFTRL 540
           LFGLKKFGALYNFITLSTPMGSL+FSGLIASSIYDSEAEKQARNHLTQFQSSSS WFTRL
Sbjct: 481 LFGLKKFGALYNFITLSTPMGSLVFSGLIASSIYDSEAEKQARNHLTQFQSSSSFWFTRL 540

Query: 541 YAEGPHKCEGAICFFLTCMIMAGFCAIAGILSLILVYRTKGVYHNLYGKSRTSTLS 597
           Y EGPHKCEGAICFFLTCMIM GFCAIA ILSLILV+RTKGVYHNLYGKSRTSTLS
Sbjct: 541 YTEGPHKCEGAICFFLTCMIMGGFCAIAAILSLILVHRTKGVYHNLYGKSRTSTLS 596

BLAST of IVF0000244 vs. ExPASy TrEMBL
Match: A0A6J1E533 (protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucurbita moschata OX=3662 GN=LOC111430673 PE=4 SV=1)

HSP 1 Score: 1045.8 bits (2703), Expect = 6.8e-302
Identity = 535/596 (89.77%), Postives = 568/596 (95.30%), Query Frame = 0

Query: 1   MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPVIKTNLSYNQRQVSRLGVAK 60
           MGR N+KLVAF+NNRWLVFVAAIW+QS AGIGYLFGSISPVIKTNLSYNQ+Q++RLGVAK
Sbjct: 1   MGRVNEKLVAFLNNRWLVFVAAIWVQSCAGIGYLFGSISPVIKTNLSYNQKQIARLGVAK 60

Query: 61  DLGDSVGFLAATLTEILPFWGSLLVGAIHNIVGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120
           DLGDSVG LA TL+EILPFWG+LLVGA++N +GYGWVWLIVTGRAPVLPLWAMCVLVFVG
Sbjct: 61  DLGDSVGILAGTLSEILPFWGTLLVGALNNFIGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120

Query: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIIHSPDSANLIFMV 180
           TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAI+H PDSANLIFM+
Sbjct: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIMHFPDSANLIFMI 180

Query: 181 AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLIEDLVTLSP 240
           AVGPALVAIG+MFFIRPVAGHRQVRPSDG+SFSSVYGVCLLLAAYLMGVML+EDLV LSP
Sbjct: 181 AVGPALVAIGMMFFIRPVAGHRQVRPSDGVSFSSVYGVCLLLAAYLMGVMLVEDLVDLSP 240

Query: 241 TVITIFTVVMFVILLTPFLIPVTLTFSSETTTYAEQEALLPPSEKEEPARTEPDGNEVIF 300
           TVI IFT VMFVILLTPFLIPV LTFSSETT Y EQEALL  S K+EPAR+EPDG+EVIF
Sbjct: 241 TVIAIFTAVMFVILLTPFLIPVILTFSSETTAYPEQEALLQQSPKQEPARSEPDGHEVIF 300

Query: 301 SEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360
           SEVEDEKSEGEDLLPASERQKRIAQLQA+LLQAAAEGAVRVKRRKGPRRGEDFTLGQALI
Sbjct: 301 SEVEDEKSEGEDLLPASERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360

Query: 361 KADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSE 420
           KADFWLIF S LLGSGTGLTVIDNLGQMSQSLGY+NTHIFVSLISIWNFLGRVGGGY SE
Sbjct: 361 KADFWLIFFSLLLGSGTGLTVIDNLGQMSQSLGYNNTHIFVSLISIWNFLGRVGGGYFSE 420

Query: 421 IVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480
           IVVRD+AYPRPIAM  AQ LMIFGH+FIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE
Sbjct: 421 IVVRDYAYPRPIAMATAQFLMIFGHIFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480

Query: 481 LFGLKKFGALYNFITLSTPMGSLIFSGLIASSIYDSEAEKQARNHLTQFQSSSSLWFTRL 540
           LFGLKKFGALYNF+TLSTPMGSLIFSGLIASSIYDSEAEKQA N L+Q QSSSS+WF+RL
Sbjct: 481 LFGLKKFGALYNFLTLSTPMGSLIFSGLIASSIYDSEAEKQAHNGLSQLQSSSSVWFSRL 540

Query: 541 YAEGPHKCEGAICFFLTCMIMAGFCAIAGILSLILVYRTKGVYHNLYGKSRTSTLS 597
           + + P KC+GAICFFLTCMIMAGFCAIAG+LSLILV+RTKGVY+NLYGKSR STLS
Sbjct: 541 HVDAPLKCDGAICFFLTCMIMAGFCAIAGMLSLILVHRTKGVYYNLYGKSRASTLS 596

BLAST of IVF0000244 vs. ExPASy TrEMBL
Match: A0A6J1JLV2 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111487059 PE=4 SV=1)

HSP 1 Score: 1044.6 bits (2700), Expect = 1.5e-301
Identity = 535/596 (89.77%), Postives = 566/596 (94.97%), Query Frame = 0

Query: 1   MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPVIKTNLSYNQRQVSRLGVAK 60
           MGR N+KLVAF+NNRWLVFVAAIW+QS AGIGYLFGSISPVIKTNLSYNQ+Q++RLGVAK
Sbjct: 1   MGRVNEKLVAFLNNRWLVFVAAIWVQSCAGIGYLFGSISPVIKTNLSYNQKQIARLGVAK 60

Query: 61  DLGDSVGFLAATLTEILPFWGSLLVGAIHNIVGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120
           DLGDSVG LA TL+EILPFWG+LLVGA++N +GYGWVWLIVTGRAPVLPLWAMCVLVFVG
Sbjct: 61  DLGDSVGILAGTLSEILPFWGTLLVGALNNFIGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120

Query: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIIHSPDSANLIFMV 180
           TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAI+H PDSANLIFM+
Sbjct: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIMHFPDSANLIFMI 180

Query: 181 AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLIEDLVTLSP 240
           AVGPALVAIG+MFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVML+EDLV LSP
Sbjct: 181 AVGPALVAIGMMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLVEDLVDLSP 240

Query: 241 TVITIFTVVMFVILLTPFLIPVTLTFSSETTTYAEQEALLPPSEKEEPARTEPDGNEVIF 300
           TVI IFT VMFVILLTPFLIPV LTFSSET  Y EQEALL  S K+EPAR+EPDG+EVIF
Sbjct: 241 TVIAIFTAVMFVILLTPFLIPVILTFSSETMAYPEQEALLQQSPKQEPARSEPDGHEVIF 300

Query: 301 SEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360
           SEVEDEKSEGEDLLPA ERQKRIAQLQA+LLQAAAEGAVRVKRRKGPRRGEDFTLGQALI
Sbjct: 301 SEVEDEKSEGEDLLPALERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360

Query: 361 KADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSE 420
           KADFWLIF S LLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGY SE
Sbjct: 361 KADFWLIFFSLLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYFSE 420

Query: 421 IVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480
           IVVRD+AYPRPIAM  AQ LMIFGH+FIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE
Sbjct: 421 IVVRDYAYPRPIAMATAQFLMIFGHIFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480

Query: 481 LFGLKKFGALYNFITLSTPMGSLIFSGLIASSIYDSEAEKQARNHLTQFQSSSSLWFTRL 540
           LFGLKKFGALYNF+TLSTPMGSLIFSGLIASSIYDSEAEKQA N L+Q QSSSS+WF+RL
Sbjct: 481 LFGLKKFGALYNFLTLSTPMGSLIFSGLIASSIYDSEAEKQAHNGLSQLQSSSSVWFSRL 540

Query: 541 YAEGPHKCEGAICFFLTCMIMAGFCAIAGILSLILVYRTKGVYHNLYGKSRTSTLS 597
           + + P KC+GAICFFLTCMIMAGFCAIAG+LSLILV+RTKGVY+NLYGKSR STLS
Sbjct: 541 HVDAPLKCDGAICFFLTCMIMAGFCAIAGMLSLILVHRTKGVYYNLYGKSRASTLS 596

BLAST of IVF0000244 vs. NCBI nr
Match: XP_008441326.1 (PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4 isoform X1 [Cucumis melo] >KAA0056548.1 protein NUCLEAR FUSION DEFECTIVE 4 isoform X1 [Cucumis melo var. makuwa] >TYK02714.1 protein NUCLEAR FUSION DEFECTIVE 4 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1155 bits (2988), Expect = 0.0
Identity = 596/596 (100.00%), Postives = 596/596 (100.00%), Query Frame = 0

Query: 1   MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPVIKTNLSYNQRQVSRLGVAK 60
           MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPVIKTNLSYNQRQVSRLGVAK
Sbjct: 1   MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPVIKTNLSYNQRQVSRLGVAK 60

Query: 61  DLGDSVGFLAATLTEILPFWGSLLVGAIHNIVGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120
           DLGDSVGFLAATLTEILPFWGSLLVGAIHNIVGYGWVWLIVTGRAPVLPLWAMCVLVFVG
Sbjct: 61  DLGDSVGFLAATLTEILPFWGSLLVGAIHNIVGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120

Query: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIIHSPDSANLIFMV 180
           TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIIHSPDSANLIFMV
Sbjct: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIIHSPDSANLIFMV 180

Query: 181 AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLIEDLVTLSP 240
           AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLIEDLVTLSP
Sbjct: 181 AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLIEDLVTLSP 240

Query: 241 TVITIFTVVMFVILLTPFLIPVTLTFSSETTTYAEQEALLPPSEKEEPARTEPDGNEVIF 300
           TVITIFTVVMFVILLTPFLIPVTLTFSSETTTYAEQEALLPPSEKEEPARTEPDGNEVIF
Sbjct: 241 TVITIFTVVMFVILLTPFLIPVTLTFSSETTTYAEQEALLPPSEKEEPARTEPDGNEVIF 300

Query: 301 SEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360
           SEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI
Sbjct: 301 SEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360

Query: 361 KADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSE 420
           KADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSE
Sbjct: 361 KADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSE 420

Query: 421 IVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480
           IVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE
Sbjct: 421 IVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480

Query: 481 LFGLKKFGALYNFITLSTPMGSLIFSGLIASSIYDSEAEKQARNHLTQFQSSSSLWFTRL 540
           LFGLKKFGALYNFITLSTPMGSLIFSGLIASSIYDSEAEKQARNHLTQFQSSSSLWFTRL
Sbjct: 481 LFGLKKFGALYNFITLSTPMGSLIFSGLIASSIYDSEAEKQARNHLTQFQSSSSLWFTRL 540

Query: 541 YAEGPHKCEGAICFFLTCMIMAGFCAIAGILSLILVYRTKGVYHNLYGKSRTSTLS 596
           YAEGPHKCEGAICFFLTCMIMAGFCAIAGILSLILVYRTKGVYHNLYGKSRTSTLS
Sbjct: 541 YAEGPHKCEGAICFFLTCMIMAGFCAIAGILSLILVYRTKGVYHNLYGKSRTSTLS 596

BLAST of IVF0000244 vs. NCBI nr
Match: XP_011649956.1 (protein NUCLEAR FUSION DEFECTIVE 4 isoform X1 [Cucumis sativus] >KGN63164.1 hypothetical protein Csa_021870 [Cucumis sativus])

HSP 1 Score: 1126 bits (2912), Expect = 0.0
Identity = 576/596 (96.64%), Postives = 584/596 (97.99%), Query Frame = 0

Query: 1   MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPVIKTNLSYNQRQVSRLGVAK 60
           MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISP+IKTNLSYNQRQ+SRLGVAK
Sbjct: 1   MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPIIKTNLSYNQRQISRLGVAK 60

Query: 61  DLGDSVGFLAATLTEILPFWGSLLVGAIHNIVGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120
           DLGDSVGFLAATLTEILPFWGSLLVGAIHN VGYGWVWLIVTGRAPVLPLWAMC LVF+G
Sbjct: 61  DLGDSVGFLAATLTEILPFWGSLLVGAIHNFVGYGWVWLIVTGRAPVLPLWAMCALVFIG 120

Query: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIIHSPDSANLIFMV 180
           TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQ YAI HSP+SANLIFMV
Sbjct: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQTYAIFHSPESANLIFMV 180

Query: 181 AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLIEDLVTLSP 240
           AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSF+SVYGVCLLLAAYLMGVMLIEDLVTLSP
Sbjct: 181 AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFTSVYGVCLLLAAYLMGVMLIEDLVTLSP 240

Query: 241 TVITIFTVVMFVILLTPFLIPVTLTFSSETTTYAEQEALLPPSEKEEPARTEPDGNEVIF 300
            VITIFTVVMFVILLTPF IPV+LT SSE TTYAEQEALLPPSEKEEPARTEPDGNEVIF
Sbjct: 241 IVITIFTVVMFVILLTPFFIPVSLTLSSEATTYAEQEALLPPSEKEEPARTEPDGNEVIF 300

Query: 301 SEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360
           SEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI
Sbjct: 301 SEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360

Query: 361 KADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSE 420
           KADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSE
Sbjct: 361 KADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSE 420

Query: 421 IVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480
           IVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE
Sbjct: 421 IVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480

Query: 481 LFGLKKFGALYNFITLSTPMGSLIFSGLIASSIYDSEAEKQARNHLTQFQSSSSLWFTRL 540
           LFGLKKFGALYNFITLSTPMGSL+FSGLIASSIYDSEAEKQARNHLTQFQSSSS WFTRL
Sbjct: 481 LFGLKKFGALYNFITLSTPMGSLVFSGLIASSIYDSEAEKQARNHLTQFQSSSSFWFTRL 540

Query: 541 YAEGPHKCEGAICFFLTCMIMAGFCAIAGILSLILVYRTKGVYHNLYGKSRTSTLS 596
           Y EGPHKCEGAICFFLTCMIM GFCAIA ILSLILV+RTKGVYHNLYGKSRTSTLS
Sbjct: 541 YTEGPHKCEGAICFFLTCMIMGGFCAIAAILSLILVHRTKGVYHNLYGKSRTSTLS 596

BLAST of IVF0000244 vs. NCBI nr
Match: XP_038885267.1 (protein NUCLEAR FUSION DEFECTIVE 4 isoform X1 [Benincasa hispida])

HSP 1 Score: 1066 bits (2757), Expect = 0.0
Identity = 546/596 (91.61%), Postives = 570/596 (95.64%), Query Frame = 0

Query: 1   MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPVIKTNLSYNQRQVSRLGVAK 60
           MGRWN+KLVAF NNRWLVFVAAIWLQS AGIGYLFGSISPVIKTNLSYNQRQ++RLGVAK
Sbjct: 1   MGRWNEKLVAFFNNRWLVFVAAIWLQSCAGIGYLFGSISPVIKTNLSYNQRQIARLGVAK 60

Query: 61  DLGDSVGFLAATLTEILPFWGSLLVGAIHNIVGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120
           DLGDSVG LAATL+EILPFWGSLLVGAIHN VGYGW+WLIVTGRAPVLPLWAMC+LVF+G
Sbjct: 61  DLGDSVGILAATLSEILPFWGSLLVGAIHNFVGYGWIWLIVTGRAPVLPLWAMCILVFLG 120

Query: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIIHSPDSANLIFMV 180
           TNGETYFNTV+LVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAI+H+PDSANLIFMV
Sbjct: 121 TNGETYFNTVALVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIVHAPDSANLIFMV 180

Query: 181 AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLIEDLVTLSP 240
           AVGPALVAIGVMFFIRPVAGHRQVRPSD MSF+SVYGVCLLLAAYLMGVML+EDLVTL+P
Sbjct: 181 AVGPALVAIGVMFFIRPVAGHRQVRPSDSMSFTSVYGVCLLLAAYLMGVMLVEDLVTLNP 240

Query: 241 TVITIFTVVMFVILLTPFLIPVTLTFSSETTTYAEQEALLPPSEKEEPARTEPDGNEVIF 300
           TVI IFT VMFVILLTPFLIPVTLTFSSET TYAEQEALLPPSEK+EPAR+EPDGNEVIF
Sbjct: 241 TVIAIFTGVMFVILLTPFLIPVTLTFSSETMTYAEQEALLPPSEKQEPARSEPDGNEVIF 300

Query: 301 SEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360
           SEVEDEKSEGEDLLPA ERQKRIAQLQA+LLQAAAEGAVRVKRRKGPRRGEDFTLGQALI
Sbjct: 301 SEVEDEKSEGEDLLPALERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360

Query: 361 KADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSE 420
           KADFWLIF S LLGSGTGLTVIDNLGQMS+SLGYDNTHIFVSLISIWNFLGRVGGGYLSE
Sbjct: 361 KADFWLIFVSLLLGSGTGLTVIDNLGQMSESLGYDNTHIFVSLISIWNFLGRVGGGYLSE 420

Query: 421 IVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480
           IVVRDFAYPRPIAM IAQ LMIFGH+FIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE
Sbjct: 421 IVVRDFAYPRPIAMAIAQFLMIFGHLFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480

Query: 481 LFGLKKFGALYNFITLSTPMGSLIFSGLIASSIYDSEAEKQARNHLTQFQSSSSLWFTRL 540
           LFGLKKFGALYNF+TLS PMGSLIFS LIAS IYDSEAEKQA NH    QSSSS   +RL
Sbjct: 481 LFGLKKFGALYNFLTLSNPMGSLIFSSLIASGIYDSEAEKQAHNHPPHLQSSSSFLLSRL 540

Query: 541 YAEGPHKCEGAICFFLTCMIMAGFCAIAGILSLILVYRTKGVYHNLYGKSRTSTLS 596
           YA+GPH+CEGAICFFLTCM+MAG CAIAG+LSLILVYRTKGVY NLYGKSRTSTLS
Sbjct: 541 YADGPHECEGAICFFLTCMLMAGLCAIAGMLSLILVYRTKGVYSNLYGKSRTSTLS 596

BLAST of IVF0000244 vs. NCBI nr
Match: XP_023544039.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1046 bits (2706), Expect = 0.0
Identity = 537/596 (90.10%), Postives = 567/596 (95.13%), Query Frame = 0

Query: 1   MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPVIKTNLSYNQRQVSRLGVAK 60
           MGR N+KLVAF+NNRWLVFVAAIW+QS AGIGYLFGSISPVIKTNLSYNQ+Q++RLGVAK
Sbjct: 1   MGRVNEKLVAFLNNRWLVFVAAIWIQSCAGIGYLFGSISPVIKTNLSYNQKQIARLGVAK 60

Query: 61  DLGDSVGFLAATLTEILPFWGSLLVGAIHNIVGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120
           DLGDSVG LA TL+EILPFWG+LLVGA++N +GYGWVWLIVTGRAPVLPLWAMCVLVFVG
Sbjct: 61  DLGDSVGILAGTLSEILPFWGTLLVGALNNFIGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120

Query: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIIHSPDSANLIFMV 180
           TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAI+H PDSANLIFM+
Sbjct: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIMHFPDSANLIFMI 180

Query: 181 AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLIEDLVTLSP 240
           AVGPALVAIG+MFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVML+EDLV LSP
Sbjct: 181 AVGPALVAIGMMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLVEDLVDLSP 240

Query: 241 TVITIFTVVMFVILLTPFLIPVTLTFSSETTTYAEQEALLPPSEKEEPARTEPDGNEVIF 300
           TVI IFT VMFVILLTPFLIPV LTFSSETT Y EQEALL  S K EPAR+EPDG+EVIF
Sbjct: 241 TVIAIFTAVMFVILLTPFLIPVILTFSSETTAYPEQEALLQQSPKHEPARSEPDGHEVIF 300

Query: 301 SEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360
           SEVEDEKSEGEDLLPASERQKRIAQLQA+LLQAAAEGAVRVKRRKGPRRGEDFTLGQALI
Sbjct: 301 SEVEDEKSEGEDLLPASERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360

Query: 361 KADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSE 420
           KADFWLIF S LLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGY SE
Sbjct: 361 KADFWLIFFSLLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYFSE 420

Query: 421 IVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480
           IVVRD+AYPRPIAM  AQ LMIFGH+FIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE
Sbjct: 421 IVVRDYAYPRPIAMATAQFLMIFGHIFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480

Query: 481 LFGLKKFGALYNFITLSTPMGSLIFSGLIASSIYDSEAEKQARNHLTQFQSSSSLWFTRL 540
           LFGLKKFGALYNF+TLSTPMGSLIFSGLIASSIYDSEAEKQA N L+Q QSSSS+WF+RL
Sbjct: 481 LFGLKKFGALYNFLTLSTPMGSLIFSGLIASSIYDSEAEKQAHNGLSQLQSSSSVWFSRL 540

Query: 541 YAEGPHKCEGAICFFLTCMIMAGFCAIAGILSLILVYRTKGVYHNLYGKSRTSTLS 596
           + + P KC+GAICFFLTCMIMAGFCAIAG+LSLILV+RTKGVY+NLYGKSR STLS
Sbjct: 541 HVDAPLKCDGAICFFLTCMIMAGFCAIAGMLSLILVHRTKGVYYNLYGKSRASTLS 596

BLAST of IVF0000244 vs. NCBI nr
Match: KAG6602685.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1045 bits (2703), Expect = 0.0
Identity = 536/596 (89.93%), Postives = 568/596 (95.30%), Query Frame = 0

Query: 1   MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPVIKTNLSYNQRQVSRLGVAK 60
           MGR N+KLVAF+NNRWLVFVAAIW+QS AGIGYLFGSISPVIKTNLSYNQ+Q++RLGVAK
Sbjct: 1   MGRVNEKLVAFLNNRWLVFVAAIWVQSCAGIGYLFGSISPVIKTNLSYNQKQIARLGVAK 60

Query: 61  DLGDSVGFLAATLTEILPFWGSLLVGAIHNIVGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120
           DLGDSVG LA TL+EILPFWG+LLVGA++N +GYGWVWLIVTGRAPVLPLWAMCVLVFVG
Sbjct: 61  DLGDSVGILAGTLSEILPFWGTLLVGALNNFIGYGWVWLIVTGRAPVLPLWAMCVLVFVG 120

Query: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIIHSPDSANLIFMV 180
           TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAI+H PDSANLIFM+
Sbjct: 121 TNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIMHFPDSANLIFMI 180

Query: 181 AVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLIEDLVTLSP 240
           AVGPALVAIG+MFFIRPVAGHRQVRPSDG+SFSSVYGVCLLLAAYLMGVML+EDLV LSP
Sbjct: 181 AVGPALVAIGMMFFIRPVAGHRQVRPSDGVSFSSVYGVCLLLAAYLMGVMLVEDLVDLSP 240

Query: 241 TVITIFTVVMFVILLTPFLIPVTLTFSSETTTYAEQEALLPPSEKEEPARTEPDGNEVIF 300
           TVI IFT VMFVILLTPFLIPV LTFSSETT Y EQEALL  S K+EPAR+EPDG+EVIF
Sbjct: 241 TVIAIFTAVMFVILLTPFLIPVILTFSSETTAYPEQEALLQQSPKQEPARSEPDGHEVIF 300

Query: 301 SEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360
           SEVEDEKSEGEDLLPASERQKRIAQLQA+LLQAAAEGAVRVKRRKGPRRGEDFTLGQALI
Sbjct: 301 SEVEDEKSEGEDLLPASERQKRIAQLQARLLQAAAEGAVRVKRRKGPRRGEDFTLGQALI 360

Query: 361 KADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSE 420
           KADFWLIF S LLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGY SE
Sbjct: 361 KADFWLIFFSLLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYFSE 420

Query: 421 IVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480
           IVVRD+AYPRPIAM  AQ LMIFGH+FIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE
Sbjct: 421 IVVRDYAYPRPIAMATAQFLMIFGHIFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASE 480

Query: 481 LFGLKKFGALYNFITLSTPMGSLIFSGLIASSIYDSEAEKQARNHLTQFQSSSSLWFTRL 540
           LFGLKKFGALYNF+TLSTPMGSLIFSGLIASSIYDSEAEKQA N L+Q QSSSS+WF+RL
Sbjct: 481 LFGLKKFGALYNFLTLSTPMGSLIFSGLIASSIYDSEAEKQAHNGLSQLQSSSSVWFSRL 540

Query: 541 YAEGPHKCEGAICFFLTCMIMAGFCAIAGILSLILVYRTKGVYHNLYGKSRTSTLS 596
           + + P KC+GAICFFLTCMIMAGFCAIAG+LSLILV+RTKGVY+NLYGKSR STLS
Sbjct: 541 HVDAPLKCDGAICFFLTCMIMAGFCAIAGMLSLILVHRTKGVYYNLYGKSRASTLS 596

BLAST of IVF0000244 vs. TAIR 10
Match: AT5G14120.1 (Major facilitator superfamily protein )

HSP 1 Score: 813.1 bits (2099), Expect = 1.4e-235
Identity = 415/589 (70.46%), Postives = 494/589 (83.87%), Query Frame = 0

Query: 6   DKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPVIKTNLSYNQRQVSRLGVAKDLGDS 65
           +K V+FINNRWLVFVAA+W+QS AGIGYLFGSISPVIK++L+YNQ+++SRLGVAKDLGDS
Sbjct: 7   EKFVSFINNRWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKELSRLGVAKDLGDS 66

Query: 66  VGFLAATLTEILPFWGSLLVGAIHNIVGYGWVWLIVTGRAPVLPLWAMCVLVFVGTNGET 125
           VGF+A TL+EILP W +LLVGA+ N++GYGWVWLIVTGRAP+LPLWAMCVL+FVG NGET
Sbjct: 67  VGFIAGTLSEILPLWAALLVGAVQNLIGYGWVWLIVTGRAPILPLWAMCVLIFVGNNGET 126

Query: 126 YFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIIHSPDSANLIFMVAVGPA 185
           YFNT +LVS VQNFPKSRGPVVGILKGFAGL GAI++QIY +IHS + A+LI MVAV PA
Sbjct: 127 YFNTGALVSGVQNFPKSRGPVVGILKGFAGLGGAIISQIYTMIHSSNPASLILMVAVTPA 186

Query: 186 LVAIGVMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLIEDLVTLSPTVITI 245
           +V + +MFFIRPV GH+Q+RP+DG SF+ +YGVCLLLAAYLM VMLI+DLV +S  VIT+
Sbjct: 187 VVVVCLMFFIRPVGGHKQIRPTDGASFTFIYGVCLLLAAYLMSVMLIQDLVVVSHNVITV 246

Query: 246 FTVVMFVILLTPFLIPVTLTFSSETTTYAE--QEALLPPSEKEEPARTEPDGNEVIFSEV 305
           FT+V+FVIL+ P L+P+  +F +ET    +  +E L+P  E +EP    PD   +I SEV
Sbjct: 247 FTIVLFVILVVPILVPIMTSFFTETNEPDDTIEEPLVPKREDQEPGLQTPD---LILSEV 306

Query: 306 EDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALIKAD 365
           EDEK +  DLLPASER KRIA LQA+L+QAAAEGAVRV RR+GP RGEDFTL QAL+KAD
Sbjct: 307 EDEKPKDVDLLPASERHKRIAHLQAQLMQAAAEGAVRVNRRRGPHRGEDFTLTQALVKAD 366

Query: 366 FWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSEIVV 425
           FWLIF S LLGSG+GLTVIDNLGQMSQSLGYDNTH+ VS+ISIWNFLGR+GGGY SE+VV
Sbjct: 367 FWLIFFSLLLGSGSGLTVIDNLGQMSQSLGYDNTHVLVSMISIWNFLGRIGGGYFSELVV 426

Query: 426 RDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASELFG 485
           RD+AYPRP+AM +AQ++M  GH+F   GWPGAMYIGTL+ GLGYGAHWAIVPATASELFG
Sbjct: 427 RDYAYPRPVAMAVAQLIMSVGHIFFAYGWPGAMYIGTLLIGLGYGAHWAIVPATASELFG 486

Query: 486 LKKFGALYNFITLSTPMGSLIFSGLIASSIYDSEAEKQARNHLTQFQSSSSLWFTRLYAE 545
           LKKFGALYNF+TL+ P GSL+FSG+IASSIYD EAE+QA  H + F    +L        
Sbjct: 487 LKKFGALYNFLTLANPAGSLVFSGMIASSIYDREAERQA--HGSVFDPDDAL-------- 546

Query: 546 GPHKCEGAICFFLTCMIMAGFCAIAGILSLILVYRTKGVYHNLYGKSRT 593
              +C G+ICFFLT +IM+GFC IA +LS+ILV RTK VY +LYGK+RT
Sbjct: 547 ---RCNGSICFFLTSLIMSGFCIIACMLSMILVRRTKSVYTHLYGKTRT 579

BLAST of IVF0000244 vs. TAIR 10
Match: AT3G01930.2 (Major facilitator superfamily protein )

HSP 1 Score: 801.2 bits (2068), Expect = 5.6e-232
Identity = 411/590 (69.66%), Postives = 490/590 (83.05%), Query Frame = 0

Query: 6   DKLVAFINNRWLVFVAAIWLQSWAGIGYLFGSISPVIKTNLSYNQRQVSRLGVAKDLGDS 65
           +++ +FINNRWLVFVAA+W+QS AGIGYLFGSISPVIK++L+YNQ+Q+SRLGVAKDLGDS
Sbjct: 7   ERVKSFINNRWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKQLSRLGVAKDLGDS 66

Query: 66  VGFLAATLTEILPFWGSLLVGAIHNIVGYGWVWLIVTGRAPVLPLWAMCVLVFVGTNGET 125
           VGFLA TL+EILP W +LLVG++ N+VGYGWVWLIVTGRAP+LPLWAMC+L+FVG NGET
Sbjct: 67  VGFLAGTLSEILPLWAALLVGSVQNLVGYGWVWLIVTGRAPILPLWAMCILIFVGNNGET 126

Query: 126 YFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIIHSPDSANLIFMVAVGPA 185
           YFNT +LVS VQNFPKSRGPVVGILKGFAGL GAIL+Q+Y +IHS D A+LIFMVAV P+
Sbjct: 127 YFNTAALVSGVQNFPKSRGPVVGILKGFAGLGGAILSQVYTMIHSSDRASLIFMVAVAPS 186

Query: 186 LVAIGVMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLIEDLVTLSPTVITI 245
           +V + +MFFIRPV GHRQ+R SD  SF+ +Y VC+LLAAYLM VML+ED + LS ++I  
Sbjct: 187 VVVVPLMFFIRPVGGHRQIRSSDATSFTVIYAVCILLAAYLMAVMLVEDFIDLSHSIIIA 246

Query: 246 FTVVMFVILLTPFLIPVTLTFSSETTTYAE--QEALLPPSEKEEPAR-TEPD-GNEVIFS 305
           FTVV+F ILL P  IP+  +  + +T   +  +E LL   + ++P + T PD G E+IFS
Sbjct: 247 FTVVLFAILLVPIFIPIATSCFTASTDPCDTLEEPLLGDQQGQDPGQSTTPDHGPELIFS 306

Query: 306 EVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQALIK 365
           EVEDEK +  DLLPA ER KRIAQLQAKL+QAAAEGAVRVKRR+GP RGEDFTL QAL+K
Sbjct: 307 EVEDEKPKEVDLLPAVERHKRIAQLQAKLMQAAAEGAVRVKRRRGPHRGEDFTLTQALVK 366

Query: 366 ADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYLSEI 425
           ADFWLIF S LLGSG+GLTVIDNLGQMSQSLGYDNTH+FVS+ISIWNFLGR+GGGY SE+
Sbjct: 367 ADFWLIFFSLLLGSGSGLTVIDNLGQMSQSLGYDNTHVFVSMISIWNFLGRIGGGYFSEL 426

Query: 426 VVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATASEL 485
           +VRD+AYPRP+A+ +AQ++M  GH+F   GWPGAM+IGTL+ GLGYGAHWAIVPATASEL
Sbjct: 427 IVRDYAYPRPVAIAVAQLVMSVGHIFFAYGWPGAMHIGTLLIGLGYGAHWAIVPATASEL 486

Query: 486 FGLKKFGALYNFITLSTPMGSLIFSGLIASSIYDSEAEKQARNHLTQFQSSSSLWFTRLY 545
           FGLKKFGALYNF+TL+ P GSL+FSGLIASSIYD EAE+QA+  L  F     L      
Sbjct: 487 FGLKKFGALYNFLTLANPAGSLVFSGLIASSIYDREAERQAQGSL--FNPDDVL------ 546

Query: 546 AEGPHKCEGAICFFLTCMIMAGFCAIAGILSLILVYRTKGVYHNLYGKSR 592
                +C G+IC+FLT +IM+GFC IA  LS+ILV RTK VY NLYGK+R
Sbjct: 547 -----RCRGSICYFLTSLIMSGFCLIAAALSMILVQRTKPVYTNLYGKTR 583

BLAST of IVF0000244 vs. TAIR 10
Match: AT3G01930.1 (Major facilitator superfamily protein )

HSP 1 Score: 620.2 bits (1598), Expect = 1.8e-177
Identity = 327/483 (67.70%), Postives = 389/483 (80.54%), Query Frame = 0

Query: 113 MCVLVFVGTNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIIHSPD 172
           MC+L+FVG NGETYFNT +LVS VQNFPKSRGPVVGILKGFAGL GAIL+Q+Y +IHS D
Sbjct: 1   MCILIFVGNNGETYFNTAALVSGVQNFPKSRGPVVGILKGFAGLGGAILSQVYTMIHSSD 60

Query: 173 SANLIFMVAVGPALVAIGVMFFIRPVAGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLI 232
            A+LIFMVAV P++V + +MFFIRPV GHRQ+R SD  SF+ +Y VC+LLAAYLM VML+
Sbjct: 61  RASLIFMVAVAPSVVVVPLMFFIRPVGGHRQIRSSDATSFTVIYAVCILLAAYLMAVMLV 120

Query: 233 EDLVTLSPTVITIFTVVMFVILLTPFLIPVTLTFSSETTTYAE--QEALLPPSEKEEPAR 292
           ED + LS ++I  FTVV+F ILL P  IP+  +  + +T   +  +E LL   + ++P +
Sbjct: 121 EDFIDLSHSIIIAFTVVLFAILLVPIFIPIATSCFTASTDPCDTLEEPLLGDQQGQDPGQ 180

Query: 293 -TEPD-GNEVIFSEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPR 352
            T PD G E+IFSEVEDEK +  DLLPA ER KRIAQLQAKL+QAAAEGAVRVKRR+GP 
Sbjct: 181 STTPDHGPELIFSEVEDEKPKEVDLLPAVERHKRIAQLQAKLMQAAAEGAVRVKRRRGPH 240

Query: 353 RGEDFTLGQALIKADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWN 412
           RGEDFTL QAL+KADFWLIF S LLGSG+GLTVIDNLGQMSQSLGYDNTH+FVS+ISIWN
Sbjct: 241 RGEDFTLTQALVKADFWLIFFSLLLGSGSGLTVIDNLGQMSQSLGYDNTHVFVSMISIWN 300

Query: 413 FLGRVGGGYLSEIVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYG 472
           FLGR+GGGY SE++VRD+AYPRP+A+ +AQ++M  GH+F   GWPGAM+IGTL+ GLGYG
Sbjct: 301 FLGRIGGGYFSELIVRDYAYPRPVAIAVAQLVMSVGHIFFAYGWPGAMHIGTLLIGLGYG 360

Query: 473 AHWAIVPATASELFGLKKFGALYNFITLSTPMGSLIFSGLIASSIYDSEAEKQARNHLTQ 532
           AHWAIVPATASELFGLKKFGALYNF+TL+ P GSL+FSGLIASSIYD EAE+QA+  L  
Sbjct: 361 AHWAIVPATASELFGLKKFGALYNFLTLANPAGSLVFSGLIASSIYDREAERQAQGSL-- 420

Query: 533 FQSSSSLWFTRLYAEGPHKCEGAICFFLTCMIMAGFCAIAGILSLILVYRTKGVYHNLYG 592
           F     L           +C G+IC+FLT +IM+GFC IA  LS+ILV RTK VY NLYG
Sbjct: 421 FNPDDVL-----------RCRGSICYFLTSLIMSGFCLIAAALSMILVQRTKPVYTNLYG 470

BLAST of IVF0000244 vs. TAIR 10
Match: AT5G50630.1 (Major facilitator superfamily protein )

HSP 1 Score: 505.8 bits (1301), Expect = 4.9e-143
Identity = 272/589 (46.18%), Postives = 384/589 (65.20%), Query Frame = 0

Query: 1   MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLF-GSISPVIKTNLSYNQRQVSRLGVA 60
           M  W  KL   +N+RWLVFV A+W+QS AG+GYLF GS+SP IKT+L YNQ+Q++ LGVA
Sbjct: 2   MTLWRHKLELLVNDRWLVFVCAMWVQSVAGVGYLFGGSMSPAIKTSLGYNQKQIALLGVA 61

Query: 61  KDLGDSVGFLAATLTEILPFWGSLLVGAIHNIVGYGWVWLIVTGRAPVLPLWAMCVLVFV 120
           K+LGD++GF++  L+E+ P W  LLVGA  N+ GYG VWL+VTG+ P LPLW + V +FV
Sbjct: 62  KNLGDAIGFVSGALSEVSPSWVVLLVGATQNLFGYGVVWLVVTGQLPNLPLWMLFVAIFV 121

Query: 121 GTNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIIHSPDSANLIFM 180
           GTNGETY+NT SLVSC+ NFP+SRGPVVGILKGF+GLSGAILTQ+Y + +    +++I M
Sbjct: 122 GTNGETYYNTASLVSCIHNFPESRGPVVGILKGFSGLSGAILTQVYLMFNPSHDSSVILM 181

Query: 181 VAVGPALVAIGVMFFIRPV-AGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLIEDLVTL 240
           VA+GP +V + ++F +RPV    R    SD + F ++YG C++LA YL+G+++++ +  +
Sbjct: 182 VALGPPVVVLALLFIVRPVERSCRTNLRSDDLRFLAIYGFCVVLAVYLLGLLVLQSVFDM 241

Query: 241 SPTVITIFTVVMFVILLTPFLIPVTLTFSSETTTYAEQEALLPPSEKEEPARTEPDGNEV 300
           + T+IT    ++ + ++ P L+P +  F S              S K E   +  D    
Sbjct: 242 TQTIITTSGAILVIFMVVPVLVPFSSVFISGNNV---------TSVKPEEGTSNVD---- 301

Query: 301 IFSEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQA 360
                   + E   L+  S+R                      ++++ P  GEDFTL QA
Sbjct: 302 --------QHEARTLIERSDRPP--------------------EKKRAPCIGEDFTLLQA 361

Query: 361 LIKADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYL 420
           L +ADFWLIF S +LG G+G+T+IDNLGQ+  SLGY NT IFVSLISI NFLGRV GGY 
Sbjct: 362 LGQADFWLIFMSLVLGVGSGITIIDNLGQICYSLGYSNTKIFVSLISISNFLGRVAGGYF 421

Query: 421 SEIVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATA 480
           SE+++R  + PR +AM++ Q +M  G ++  + WPG +Y+ T++ G+GYGAHWAI PA+ 
Sbjct: 422 SELIIRKLSLPRTLAMSVVQAIMSLGLIYYAIDWPGKIYVVTIVIGMGYGAHWAIAPASV 481

Query: 481 SELFGLKKFGALYNFITLSTPMGSLIFSGLIASSIYDSEAEKQARNHLTQFQSSSSLWFT 540
           S++FGLK FG+LYNF   + P+GS +FSG+IAS+IYD  A KQA    T+ +S       
Sbjct: 482 SDIFGLKSFGSLYNFQITALPIGSFVFSGVIASNIYDYYARKQA-GPTTETESL------ 534

Query: 541 RLYAEGPHKCEGAICFFLTCMIMAGFCAIAGILSLILVYRTKGVYHNLY 588
                    C G++C+ +TC +M+  C +A +LSL +VYRT+  Y  L+
Sbjct: 542 --------VCTGSVCYSVTCSLMSMLCLMAMVLSLSVVYRTRKFYLRLH 534

BLAST of IVF0000244 vs. TAIR 10
Match: AT5G50520.1 (Major facilitator superfamily protein )

HSP 1 Score: 505.8 bits (1301), Expect = 4.9e-143
Identity = 272/589 (46.18%), Postives = 384/589 (65.20%), Query Frame = 0

Query: 1   MGRWNDKLVAFINNRWLVFVAAIWLQSWAGIGYLF-GSISPVIKTNLSYNQRQVSRLGVA 60
           M  W  KL   +N+RWLVFV A+W+QS AG+GYLF GS+SP IKT+L YNQ+Q++ LGVA
Sbjct: 2   MTLWRHKLELLVNDRWLVFVCAMWVQSVAGVGYLFGGSMSPAIKTSLGYNQKQIALLGVA 61

Query: 61  KDLGDSVGFLAATLTEILPFWGSLLVGAIHNIVGYGWVWLIVTGRAPVLPLWAMCVLVFV 120
           K+LGD++GF++  L+E+ P W  LLVGA  N+ GYG VWL+VTG+ P LPLW + V +FV
Sbjct: 62  KNLGDAIGFVSGALSEVSPSWVVLLVGATQNLFGYGVVWLVVTGQLPNLPLWMLFVAIFV 121

Query: 121 GTNGETYFNTVSLVSCVQNFPKSRGPVVGILKGFAGLSGAILTQIYAIIHSPDSANLIFM 180
           GTNGETY+NT SLVSC+ NFP+SRGPVVGILKGF+GLSGAILTQ+Y + +    +++I M
Sbjct: 122 GTNGETYYNTASLVSCIHNFPESRGPVVGILKGFSGLSGAILTQVYLMFNPSHDSSVILM 181

Query: 181 VAVGPALVAIGVMFFIRPV-AGHRQVRPSDGMSFSSVYGVCLLLAAYLMGVMLIEDLVTL 240
           VA+GP +V + ++F +RPV    R    SD + F ++YG C++LA YL+G+++++ +  +
Sbjct: 182 VALGPPVVVLALLFIVRPVERSCRTNLRSDDLRFLAIYGFCVVLAVYLLGLLVLQSVFDM 241

Query: 241 SPTVITIFTVVMFVILLTPFLIPVTLTFSSETTTYAEQEALLPPSEKEEPARTEPDGNEV 300
           + T+IT    ++ + ++ P L+P +  F S              S K E   +  D    
Sbjct: 242 TQTIITTSGAILVIFMVVPVLVPFSSVFISGNNV---------TSVKPEEGTSNVD---- 301

Query: 301 IFSEVEDEKSEGEDLLPASERQKRIAQLQAKLLQAAAEGAVRVKRRKGPRRGEDFTLGQA 360
                   + E   L+  S+R                      ++++ P  GEDFTL QA
Sbjct: 302 --------QHEARTLIERSDRPP--------------------EKKRAPCIGEDFTLLQA 361

Query: 361 LIKADFWLIFSSHLLGSGTGLTVIDNLGQMSQSLGYDNTHIFVSLISIWNFLGRVGGGYL 420
           L +ADFWLIF S +LG G+G+T+IDNLGQ+  SLGY NT IFVSLISI NFLGRV GGY 
Sbjct: 362 LGQADFWLIFMSLVLGVGSGITIIDNLGQICYSLGYSNTKIFVSLISISNFLGRVAGGYF 421

Query: 421 SEIVVRDFAYPRPIAMTIAQVLMIFGHVFIGMGWPGAMYIGTLITGLGYGAHWAIVPATA 480
           SE+++R  + PR +AM++ Q +M  G ++  + WPG +Y+ T++ G+GYGAHWAI PA+ 
Sbjct: 422 SELIIRKLSLPRTLAMSVVQAIMSLGLIYYAIDWPGKIYVVTIVIGMGYGAHWAIAPASV 481

Query: 481 SELFGLKKFGALYNFITLSTPMGSLIFSGLIASSIYDSEAEKQARNHLTQFQSSSSLWFT 540
           S++FGLK FG+LYNF   + P+GS +FSG+IAS+IYD  A KQA    T+ +S       
Sbjct: 482 SDIFGLKSFGSLYNFQITALPIGSFVFSGVIASNIYDYYARKQA-GPTTETESL------ 534

Query: 541 RLYAEGPHKCEGAICFFLTCMIMAGFCAIAGILSLILVYRTKGVYHNLY 588
                    C G++C+ +TC +M+  C +A +LSL +VYRT+  Y  L+
Sbjct: 542 --------VCTGSVCYSVTCSLMSMLCLMAMVLSLSVVYRTRKFYLRLH 534

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4I9E12.4e-3025.80Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ... [more]
Match NameE-valueIdentityDescription
A0A5A7UN830.0e+00100.00Protein NUCLEAR FUSION DEFECTIVE 4 isoform X1 OS=Cucumis melo var. makuwa OX=119... [more]
A0A1S3B3570.0e+00100.00protein NUCLEAR FUSION DEFECTIVE 4 isoform X1 OS=Cucumis melo OX=3656 GN=LOC1034... [more]
A0A0A0LQ600.0e+0096.64Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G40613... [more]
A0A6J1E5336.8e-30289.77protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucurbita moschata OX=3662 GN=LOC111430673... [more]
A0A6J1JLV21.5e-30189.77protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111487... [more]
Match NameE-valueIdentityDescription
XP_008441326.10.0100.00PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4 isoform X1 [Cucumis melo] >KAA0056... [more]
XP_011649956.10.096.64protein NUCLEAR FUSION DEFECTIVE 4 isoform X1 [Cucumis sativus] >KGN63164.1 hypo... [more]
XP_038885267.10.091.61protein NUCLEAR FUSION DEFECTIVE 4 isoform X1 [Benincasa hispida][more]
XP_023544039.10.090.10protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo][more]
KAG6602685.10.089.93Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. soror... [more]
Match NameE-valueIdentityDescription
AT5G14120.11.4e-23570.46Major facilitator superfamily protein [more]
AT3G01930.25.6e-23269.66Major facilitator superfamily protein [more]
AT3G01930.11.8e-17767.70Major facilitator superfamily protein [more]
AT5G50630.14.9e-14346.18Major facilitator superfamily protein [more]
AT5G50520.14.9e-14346.18Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 316..336
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 278..297
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 4..592
NoneNo IPR availablePANTHERPTHR21576:SF136PROTEIN NUCLEAR FUSION DEFECTIVE 4-LIKEcoord: 4..592
NoneNo IPR availableCDDcd17354MFS_Mch1p_likecoord: 15..568
e-value: 1.53699E-109
score: 331.909
IPR010658Nodulin-likePFAMPF06813Nodulin-likecoord: 15..262
e-value: 6.1E-88
score: 294.5
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 365..593
e-value: 4.3E-7
score: 31.1
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 212..582
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 10..202

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0000244.1IVF0000244.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane