Homology
BLAST of HG10021867 vs. NCBI nr
Match:
XP_038892835.1 (neutral ceramidase 2-like [Benincasa hispida])
HSP 1 Score: 1514.2 bits (3919), Expect = 0.0e+00
Identity = 739/771 (95.85%), Postives = 761/771 (98.70%), Query Frame = 0
Query: 1 MELSSLFDLIVRRSLGSIWLCVYMLVLLQSNRSVFSESKYLIGLGSHDITGPAADVNMMG 60
MELSSLFDL VRRSLG+IWLC+YMLVLLQSNRSV SESKYLIGLGSHDITGPAADVNMMG
Sbjct: 1 MELSSLFDLTVRRSLGTIWLCIYMLVLLQSNRSVLSESKYLIGLGSHDITGPAADVNMMG 60
Query: 61 YANAEQIASGIHFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
YANAEQIASG+HFRLRARAFIVAEPQGKRV+FVNLDACMASQIVTIKVLERLKARYGDLY
Sbjct: 61 YANAEQIASGVHFRLRARAFIVAEPQGKRVIFVNLDACMASQIVTIKVLERLKARYGDLY 120
Query: 121 TEKNVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQAHENLLPGSI 180
TEKNVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSF+VLVDGIEKSIIQAHENLLPGSI
Sbjct: 121 TEKNVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSI 180
Query: 181 LINKGELIDAGVNRSPSAYLNNPASERSRYQYDVDKEMTLLKFVDDEWGPVGSFNWFATH 240
LINKGELIDAGVNRSPSAYLNNPASERSRY+YDVDKEMTLLKFVDDEWGPVGSFNWFATH
Sbjct: 181 LINKGELIDAGVNRSPSAYLNNPASERSRYKYDVDKEMTLLKFVDDEWGPVGSFNWFATH 240
Query: 241 GTSMSRTNSLISGDNKGAAARFMEDWFEQKGIGTLYPGESEADSIPRRVSNIIPEVYENK 300
GTSMSRTNSLISGDNKGAAARFMEDWFEQKG GT++PGESE DSIPRRVSNIIPEV++NK
Sbjct: 241 GTSMSRTNSLISGDNKGAAARFMEDWFEQKGTGTMHPGESEVDSIPRRVSNIIPEVFKNK 300
Query: 301 QELLERAASFQSRPGRPATRFLSISRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAF 360
QELLERAASFQS+PGRPATR LS+SRRVRNVLRQA+RPQFVSAFCQSNCGDVSPNTLGAF
Sbjct: 301 QELLERAASFQSQPGRPATRILSVSRRVRNVLRQANRPQFVSAFCQSNCGDVSPNTLGAF 360
Query: 361 CIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLFSKASEQLTG 420
CIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLFSKASEQLTG
Sbjct: 361 CIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLFSKASEQLTG 420
Query: 421 KVDYRHSYVDFSQLEVSLTKQGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN 480
KVD+RHSYVDFSQLEVSL+KQGGATEV++TCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN
Sbjct: 421 KVDFRHSYVDFSQLEVSLSKQGGATEVIRTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN 480
Query: 481 AFWKLVRNLLKAPGNEQIACQSPKPILLDTGEMKTPYDWAPSILPIQILRVGQLVILGVP 540
AFWKLVRNLLKAPGNEQIACQSPKPILLDTGEMK PYDWAPSILPIQILR+GQLVILGVP
Sbjct: 481 AFWKLVRNLLKAPGNEQIACQSPKPILLDTGEMKIPYDWAPSILPIQILRIGQLVILGVP 540
Query: 541 GEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTIEEYEMQRYEGA 600
GEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTT EEYEMQRYEGA
Sbjct: 541 GEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTFEEYEMQRYEGA 600
Query: 601 STLYGPHTLDAYVQEFKKLAQSLIDGRPVEPGPQPPDLLARQISLLTPVIIDVTPIGVSF 660
STLYGPHTLDAY+QEFKKLAQSLIDGRPVEPGPQPPDLLARQISLLTPVI+D TPIGVSF
Sbjct: 601 STLYGPHTLDAYIQEFKKLAQSLIDGRPVEPGPQPPDLLARQISLLTPVIMDATPIGVSF 660
Query: 661 GDVKSDVPHNSTFKRGNLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVPAYDDDDFC 720
GDVKSDVP NSTFKRGNLVTV+FWSGCPRNDLMTEGTFALVEILQKKDTWVPAYDDDDFC
Sbjct: 661 GDVKSDVPLNSTFKRGNLVTVSFWSGCPRNDLMTEGTFALVEILQKKDTWVPAYDDDDFC 720
Query: 721 LRFKWSRPSRLSPQSYATIEWRIPQTAVSGVYRIRHFGAAKIILGSIRHFT 772
LRFKWSRPSRLSPQSYATIEWRIPQTAVSGVYRIRHFGAAK +LGSIRHFT
Sbjct: 721 LRFKWSRPSRLSPQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFT 771
BLAST of HG10021867 vs. NCBI nr
Match:
KAG6583392.1 (Neutral ceramidase 1, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1478.0 bits (3825), Expect = 0.0e+00
Identity = 722/771 (93.64%), Postives = 748/771 (97.02%), Query Frame = 0
Query: 1 MELSSLFDLIVRRSLGSIWLCVYMLVLLQSNRSVFSESKYLIGLGSHDITGPAADVNMMG 60
M LSSLFD VRRSLGSIWLCVYMLV+LQSNR V SESKYLIGLGSHDITGPAADVNMMG
Sbjct: 1 MGLSSLFDHTVRRSLGSIWLCVYMLVILQSNRRVLSESKYLIGLGSHDITGPAADVNMMG 60
Query: 61 YANAEQIASGIHFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
YAN EQIASG+HFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY
Sbjct: 61 YANTEQIASGVHFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
Query: 121 TEKNVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQAHENLLPGSI 180
TE+NVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSF+VLVDGIEKSIIQAHENLLPGSI
Sbjct: 121 TEENVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSI 180
Query: 181 LINKGELIDAGVNRSPSAYLNNPASERSRYQYDVDKEMTLLKFVDDEWGPVGSFNWFATH 240
L+NKGELIDAGVNRSPSAYLNNPASER+RY+YDVDKEMTLLKF+DD+WGPVGSFNWFATH
Sbjct: 181 LVNKGELIDAGVNRSPSAYLNNPASERNRYKYDVDKEMTLLKFIDDQWGPVGSFNWFATH 240
Query: 241 GTSMSRTNSLISGDNKGAAARFMEDWFEQKGIGTLYPGESEADSIPRRVSNIIPEVYENK 300
GTSMSRTNSLISGDNKGAAARFMEDWF QKGIGTL G+SEADSIPRRVSNIIPE Y+++
Sbjct: 241 GTSMSRTNSLISGDNKGAAARFMEDWFVQKGIGTLQTGKSEADSIPRRVSNIIPEAYKDE 300
Query: 301 QELLERAASFQSRPGRPATRFLSISRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAF 360
QELL RAASFQSRPGRPATR LS+SRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAF
Sbjct: 301 QELLARAASFQSRPGRPATRILSVSRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAF 360
Query: 361 CIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLFSKASEQLTG 420
CIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLFSKASEQL G
Sbjct: 361 CIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLFSKASEQLKG 420
Query: 421 KVDYRHSYVDFSQLEVSLTKQGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN 480
VDYRHSYVDFSQLEVS+TK GGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN
Sbjct: 421 TVDYRHSYVDFSQLEVSVTKAGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN 480
Query: 481 AFWKLVRNLLKAPGNEQIACQSPKPILLDTGEMKTPYDWAPSILPIQILRVGQLVILGVP 540
AFWKLVRNLLK PG++QIACQSPKPILLDTGEMK PYDWAPSILPIQILR+GQLVILGVP
Sbjct: 481 AFWKLVRNLLKTPGSKQIACQSPKPILLDTGEMKIPYDWAPSILPIQILRIGQLVILGVP 540
Query: 541 GEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTIEEYEMQRYEGA 600
GEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTT EEYEMQRYEGA
Sbjct: 541 GEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTFEEYEMQRYEGA 600
Query: 601 STLYGPHTLDAYVQEFKKLAQSLIDGRPVEPGPQPPDLLARQISLLTPVIIDVTPIGVSF 660
STLYGPHTLDAY+QEF+KLAQSLIDGRPVEPGPQPPDLLA+QISLLTPV++D TP GVSF
Sbjct: 601 STLYGPHTLDAYIQEFRKLAQSLIDGRPVEPGPQPPDLLAKQISLLTPVVMDATPFGVSF 660
Query: 661 GDVKSDVPHNSTFKRGNLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVPAYDDDDFC 720
GDVKSDVP NSTFKRG+LVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVP+YDDDDFC
Sbjct: 661 GDVKSDVPLNSTFKRGDLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVPSYDDDDFC 720
Query: 721 LRFKWSRPSRLSPQSYATIEWRIPQTAVSGVYRIRHFGAAKIILGSIRHFT 772
LRFKWSRPSRLSPQS ATIEWRIPQTAVSGVYRIRHFGAAK +LGSIRHFT
Sbjct: 721 LRFKWSRPSRLSPQSLATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFT 771
BLAST of HG10021867 vs. NCBI nr
Match:
KAG7019159.1 (Neutral ceramidase 1 [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 1478.0 bits (3825), Expect = 0.0e+00
Identity = 722/771 (93.64%), Postives = 747/771 (96.89%), Query Frame = 0
Query: 1 MELSSLFDLIVRRSLGSIWLCVYMLVLLQSNRSVFSESKYLIGLGSHDITGPAADVNMMG 60
M LSSLFD VRRSLGSIWLCVYMLV+LQSNR V SESKYLIGLGSHDITGPAADVNMMG
Sbjct: 1 MGLSSLFDRTVRRSLGSIWLCVYMLVILQSNRRVLSESKYLIGLGSHDITGPAADVNMMG 60
Query: 61 YANAEQIASGIHFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
YAN EQIASG+HFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY
Sbjct: 61 YANTEQIASGVHFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
Query: 121 TEKNVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQAHENLLPGSI 180
TE+NVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSF+VLVDGIEKSIIQAHENLLPGSI
Sbjct: 121 TEENVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSI 180
Query: 181 LINKGELIDAGVNRSPSAYLNNPASERSRYQYDVDKEMTLLKFVDDEWGPVGSFNWFATH 240
L+NKGELIDAGVNRSPSAYLNNPASER+RY+YDVDKEMTLLKF+DD+WGPVGSFNWFATH
Sbjct: 181 LVNKGELIDAGVNRSPSAYLNNPASERNRYKYDVDKEMTLLKFIDDQWGPVGSFNWFATH 240
Query: 241 GTSMSRTNSLISGDNKGAAARFMEDWFEQKGIGTLYPGESEADSIPRRVSNIIPEVYENK 300
GTSMSRTNSLISGDNKGAAARFMEDWF QKGIGTL G+SEADSIPRRVSNIIPE Y ++
Sbjct: 241 GTSMSRTNSLISGDNKGAAARFMEDWFVQKGIGTLQTGKSEADSIPRRVSNIIPEAYRDE 300
Query: 301 QELLERAASFQSRPGRPATRFLSISRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAF 360
QELL RAASFQSRPGRPATR LS+SRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAF
Sbjct: 301 QELLARAASFQSRPGRPATRILSVSRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAF 360
Query: 361 CIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLFSKASEQLTG 420
CIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLFSKASEQL G
Sbjct: 361 CIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLFSKASEQLKG 420
Query: 421 KVDYRHSYVDFSQLEVSLTKQGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN 480
VDYRHSYVDFSQLEVS+TK GGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN
Sbjct: 421 TVDYRHSYVDFSQLEVSVTKAGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN 480
Query: 481 AFWKLVRNLLKAPGNEQIACQSPKPILLDTGEMKTPYDWAPSILPIQILRVGQLVILGVP 540
AFWKLVRNLLK PG++QIACQSPKPILLDTGEMK PYDWAPSILPIQILR+GQLVILGVP
Sbjct: 481 AFWKLVRNLLKTPGSKQIACQSPKPILLDTGEMKIPYDWAPSILPIQILRIGQLVILGVP 540
Query: 541 GEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTIEEYEMQRYEGA 600
GEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTT EEYEMQRYEGA
Sbjct: 541 GEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTFEEYEMQRYEGA 600
Query: 601 STLYGPHTLDAYVQEFKKLAQSLIDGRPVEPGPQPPDLLARQISLLTPVIIDVTPIGVSF 660
STLYGPHTLDAY+QEF+KLAQSLIDGRPVEPGPQPPDLLA+QISLLTPV++D TP GVSF
Sbjct: 601 STLYGPHTLDAYIQEFRKLAQSLIDGRPVEPGPQPPDLLAKQISLLTPVVMDATPFGVSF 660
Query: 661 GDVKSDVPHNSTFKRGNLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVPAYDDDDFC 720
GDVKSDVP NSTFKRG+LVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVP+YDDDDFC
Sbjct: 661 GDVKSDVPLNSTFKRGDLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVPSYDDDDFC 720
Query: 721 LRFKWSRPSRLSPQSYATIEWRIPQTAVSGVYRIRHFGAAKIILGSIRHFT 772
LRFKWSRPSRLSPQS ATIEWRIPQTAVSGVYRIRHFGAAK +LGSIRHFT
Sbjct: 721 LRFKWSRPSRLSPQSLATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFT 771
BLAST of HG10021867 vs. NCBI nr
Match:
XP_022964852.1 (neutral ceramidase 2-like [Cucurbita moschata])
HSP 1 Score: 1476.8 bits (3822), Expect = 0.0e+00
Identity = 721/771 (93.51%), Postives = 747/771 (96.89%), Query Frame = 0
Query: 1 MELSSLFDLIVRRSLGSIWLCVYMLVLLQSNRSVFSESKYLIGLGSHDITGPAADVNMMG 60
M LSSLFD VRRSLGSIWLCVYMLV+LQSNR V SESKYLIGLGSHDITGPAADVNMMG
Sbjct: 1 MGLSSLFDRTVRRSLGSIWLCVYMLVILQSNRRVLSESKYLIGLGSHDITGPAADVNMMG 60
Query: 61 YANAEQIASGIHFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
YAN EQIASG+HFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY
Sbjct: 61 YANTEQIASGVHFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
Query: 121 TEKNVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQAHENLLPGSI 180
TE+NVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSF+VLVDGIEKSIIQAHENLLPGSI
Sbjct: 121 TEENVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSI 180
Query: 181 LINKGELIDAGVNRSPSAYLNNPASERSRYQYDVDKEMTLLKFVDDEWGPVGSFNWFATH 240
L+NKGELIDAGVNRSPSAYLNNPASER+RY+YDVDKEMTLLKF+DD+WGPVGSFNWFATH
Sbjct: 181 LVNKGELIDAGVNRSPSAYLNNPASERNRYKYDVDKEMTLLKFIDDQWGPVGSFNWFATH 240
Query: 241 GTSMSRTNSLISGDNKGAAARFMEDWFEQKGIGTLYPGESEADSIPRRVSNIIPEVYENK 300
GTSMSRTNSLISGDNKGAAARFMEDWF QKGIGTL G+SEADSIPRRVSNIIPE Y ++
Sbjct: 241 GTSMSRTNSLISGDNKGAAARFMEDWFVQKGIGTLQTGKSEADSIPRRVSNIIPEAYNDE 300
Query: 301 QELLERAASFQSRPGRPATRFLSISRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAF 360
QELL RAASFQSRPGRPATR LS+SRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAF
Sbjct: 301 QELLARAASFQSRPGRPATRILSVSRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAF 360
Query: 361 CIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLFSKASEQLTG 420
CIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLFSKASEQL G
Sbjct: 361 CIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLFSKASEQLKG 420
Query: 421 KVDYRHSYVDFSQLEVSLTKQGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN 480
VDYRHSYVDFSQLEVS+TK GGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN
Sbjct: 421 TVDYRHSYVDFSQLEVSVTKAGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN 480
Query: 481 AFWKLVRNLLKAPGNEQIACQSPKPILLDTGEMKTPYDWAPSILPIQILRVGQLVILGVP 540
AFWKLVRNLLK PG++QIACQSPKPILLDTGEMK PYDWAPSILPIQILR+GQLVILGVP
Sbjct: 481 AFWKLVRNLLKTPGSKQIACQSPKPILLDTGEMKIPYDWAPSILPIQILRIGQLVILGVP 540
Query: 541 GEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTIEEYEMQRYEGA 600
GEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTT EEYEMQRYEGA
Sbjct: 541 GEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTFEEYEMQRYEGA 600
Query: 601 STLYGPHTLDAYVQEFKKLAQSLIDGRPVEPGPQPPDLLARQISLLTPVIIDVTPIGVSF 660
STLYGPHTLDAY+QEF+KLAQSLIDGRPVEPGPQPPDLLA+QISLLTPV++D TP GVSF
Sbjct: 601 STLYGPHTLDAYIQEFRKLAQSLIDGRPVEPGPQPPDLLAKQISLLTPVVMDATPFGVSF 660
Query: 661 GDVKSDVPHNSTFKRGNLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVPAYDDDDFC 720
GDV+SDVP NSTFKRG+LVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVP+YDDDDFC
Sbjct: 661 GDVRSDVPLNSTFKRGDLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVPSYDDDDFC 720
Query: 721 LRFKWSRPSRLSPQSYATIEWRIPQTAVSGVYRIRHFGAAKIILGSIRHFT 772
LRFKWSRPSRLSPQS ATIEWRIPQTAVSGVYRIRHFGAAK +LGSIRHFT
Sbjct: 721 LRFKWSRPSRLSPQSLATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFT 771
BLAST of HG10021867 vs. NCBI nr
Match:
XP_022970515.1 (neutral ceramidase 2-like [Cucurbita maxima])
HSP 1 Score: 1465.3 bits (3792), Expect = 0.0e+00
Identity = 718/771 (93.13%), Postives = 742/771 (96.24%), Query Frame = 0
Query: 1 MELSSLFDLIVRRSLGSIWLCVYMLVLLQSNRSVFSESKYLIGLGSHDITGPAADVNMMG 60
M LSSLFD VRRSLGSIWLCVYMLV+LQSNR V SESKYLIGLGSHDITGPAADVNMMG
Sbjct: 1 MGLSSLFDRTVRRSLGSIWLCVYMLVILQSNRRVLSESKYLIGLGSHDITGPAADVNMMG 60
Query: 61 YANAEQIASGIHFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
YAN EQIASG+HFRLRARAFIVAEPQGK+VVFVNLDACMASQIVTIKVLERLKARYGDLY
Sbjct: 61 YANTEQIASGVHFRLRARAFIVAEPQGKQVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
Query: 121 TEKNVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQAHENLLPGSI 180
TE+NVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSF+VLVDGIEKSIIQAHENLLPGSI
Sbjct: 121 TEENVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSI 180
Query: 181 LINKGELIDAGVNRSPSAYLNNPASERSRYQYDVDKEMTLLKFVDDEWGPVGSFNWFATH 240
L+NKGELIDA VNRSPSAYLNNPASER RY+YDVDKEMTLLKFVDD+WGPVGSFNWFATH
Sbjct: 181 LVNKGELIDASVNRSPSAYLNNPASERDRYKYDVDKEMTLLKFVDDQWGPVGSFNWFATH 240
Query: 241 GTSMSRTNSLISGDNKGAAARFMEDWFEQKGIGTLYPGESEADSIPRRVSNIIPEVYENK 300
GTSMSRTNSLISGDNKGAAARFMEDWF QKGI TL G+SEADSIPRRVSNIIPE Y+++
Sbjct: 241 GTSMSRTNSLISGDNKGAAARFMEDWFVQKGIRTLQTGKSEADSIPRRVSNIIPEAYKDE 300
Query: 301 QELLERAASFQSRPGRPATRFLSISRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAF 360
QELLERAA+FQSRPGRPATR LS+SRRVRN LRQADRPQFVSAFCQSNCGDVSPNTLGAF
Sbjct: 301 QELLERAAAFQSRPGRPATRILSVSRRVRNALRQADRPQFVSAFCQSNCGDVSPNTLGAF 360
Query: 361 CIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLFSKASEQLTG 420
CIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLFSKASEQL G
Sbjct: 361 CIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLFSKASEQLKG 420
Query: 421 KVDYRHSYVDFSQLEVSLTKQGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN 480
VDYRHSYVDFSQLEVS+TK GGAT VVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN
Sbjct: 421 TVDYRHSYVDFSQLEVSVTKPGGATAVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN 480
Query: 481 AFWKLVRNLLKAPGNEQIACQSPKPILLDTGEMKTPYDWAPSILPIQILRVGQLVILGVP 540
AFWKLVRNLLK PG+EQIACQSPKPILLDTGEMK PYDWAPSILPIQILR+GQLVILGVP
Sbjct: 481 AFWKLVRNLLKTPGSEQIACQSPKPILLDTGEMKIPYDWAPSILPIQILRIGQLVILGVP 540
Query: 541 GEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTIEEYEMQRYEGA 600
GEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTT EEYEMQRYEGA
Sbjct: 541 GEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTFEEYEMQRYEGA 600
Query: 601 STLYGPHTLDAYVQEFKKLAQSLIDGRPVEPGPQPPDLLARQISLLTPVIIDVTPIGVSF 660
STLYGPHTLDAY+QEFKKLAQSLIDGRPVEPGPQPPDLLA+QISLLTPV++D TP GVSF
Sbjct: 601 STLYGPHTLDAYIQEFKKLAQSLIDGRPVEPGPQPPDLLAKQISLLTPVVMDATPFGVSF 660
Query: 661 GDVKSDVPHNSTFKRGNLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVPAYDDDDFC 720
GDVKSDVP NSTFKRG+LVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVP+YDDDDFC
Sbjct: 661 GDVKSDVPLNSTFKRGDLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVPSYDDDDFC 720
Query: 721 LRFKWSRPSRLSPQSYATIEWRIPQTAVSGVYRIRHFGAAKIILGSIRHFT 772
LRFKW RPSRLSPQS ATIEWRIPQTAVSGVYRIRHFGAAK +L SIRHFT
Sbjct: 721 LRFKWWRPSRLSPQSLATIEWRIPQTAVSGVYRIRHFGAAKNLLRSIRHFT 771
BLAST of HG10021867 vs. ExPASy Swiss-Prot
Match:
F4HQM3 (Neutral ceramidase 1 OS=Arabidopsis thaliana OX=3702 GN=NCER1 PE=2 SV=1)
HSP 1 Score: 1169.8 bits (3025), Expect = 0.0e+00
Identity = 563/749 (75.17%), Postives = 640/749 (85.45%), Query Frame = 0
Query: 25 LVLLQSNRSVFSESKYLIGLGSHDITGPAADVNMMGYANAEQIASGIHFRLRARAFIVAE 84
++ L + V+S S+YLIGLGS+DITGPAADVNMMGYAN EQ+ASGIHFRLRAR FIV+E
Sbjct: 22 VLFLLTGGGVYSHSEYLIGLGSYDITGPAADVNMMGYANMEQVASGIHFRLRARTFIVSE 81
Query: 85 PQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLYTEKNVAISGIHTHAGPGGYLQYVV 144
PQGKRVVFVNLDACMASQIV +KV+ERLKARYGDLYTE+NV ISGIHTHAGPGGYLQYVV
Sbjct: 82 PQGKRVVFVNLDACMASQIVKLKVIERLKARYGDLYTEQNVGISGIHTHAGPGGYLQYVV 141
Query: 145 YIVTSLGFVRQSFNVLVDGIEKSIIQAHENLLPGSILINKGELIDAGVNRSPSAYLNNPA 204
YIVTSLGFVRQSF+ LVDGIE SIIQAHENL PGSI +N GEL+DAGVNRSPSAYLNNP+
Sbjct: 142 YIVTSLGFVRQSFDALVDGIENSIIQAHENLRPGSIFLNNGELLDAGVNRSPSAYLNNPS 201
Query: 205 SERSRYQYDVDKEMTLLKFVDDEWGPVGSFNWFATHGTSMSRTNSLISGDNKGAAARFME 264
ERS+++Y+VDKEMTLLKFVDD+WGPVGSFNWFATHGTSMSRTNSLISGDNKGAA+RFME
Sbjct: 202 KERSKHKYNVDKEMTLLKFVDDQWGPVGSFNWFATHGTSMSRTNSLISGDNKGAASRFME 261
Query: 265 DWFEQKGIGTLYPGESEADSIPRRVSNIIPEVYENKQELLERAASFQSRPGRPATRFLSI 324
DW+EQ Y E +D IPRRVS++I ++ ELLE A+ F+S+PG+P TR S
Sbjct: 262 DWYEQNTAERSYSEEFISDEIPRRVSSLIENHQDSHHELLELASYFESQPGKPVTRISSS 321
Query: 325 SRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAFCIDTGLPCDFNHSTCGGKNELCYG 384
+RRVR+ LR+AD+P FVSAFCQ+NCGDVSPN LGAFC+DTGLPCDFNHSTCGGKNE+CYG
Sbjct: 322 ARRVRSALRKADKPGFVSAFCQTNCGDVSPNVLGAFCLDTGLPCDFNHSTCGGKNEMCYG 381
Query: 385 RGPGYPDEFESTRIIGERQFRKAVDLFSKASEQLTGKVDYRHSYVDFSQLEVSLTKQGGA 444
RGPGYPDEFESTRIIGERQF+ A++LF+KASEQL GKVDYRH YVDFSQL V+L K+ G
Sbjct: 382 RGPGYPDEFESTRIIGERQFKMALELFNKASEQLQGKVDYRHVYVDFSQLNVTLPKKDGK 441
Query: 445 TEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGNAFWKLVRNLLKAPGNEQIACQSPK 504
+EVVKTCPAAMGFAFAAGTTDGPGAFDF QGDDKGN FW+LVRN+LK P +QI C PK
Sbjct: 442 SEVVKTCPAAMGFAFAAGTTDGPGAFDFTQGDDKGNPFWRLVRNVLKTPDKKQIDCHYPK 501
Query: 505 PILLDTGEMKTPYDWAPSILPIQILRVGQLVILGVPGEFTTMAGRRLRDAVKTVLTTGAK 564
PILLDTGEM PYDWAPSIL +Q+LR+GQL IL VPGEFTTMAGRRLR AVKT L
Sbjct: 502 PILLDTGEMTKPYDWAPSILSLQVLRIGQLFILSVPGEFTTMAGRRLRYAVKTQLKNSGN 561
Query: 565 REFNGNVHVVIAGLTNTYSQYVTTIEEYEMQRYEGASTLYGPHTLDAYVQEFKKLAQSLI 624
++ +G +HVVIAGL N YSQYVTT EEY++QRYEGASTLYGPHTL Y+QEFKKL++SL+
Sbjct: 562 KDLSGEIHVVIAGLANGYSQYVTTFEEYQVQRYEGASTLYGPHTLSGYIQEFKKLSKSLV 621
Query: 625 DGRPVEPGPQPPDLLARQISLLTPVIIDVTPIGVSFGDVKSDVPHNSTFKRGN-LVTVTF 684
PV+PGPQPPDLL +Q+S LTPV++D TP G SFGDV SDVP N + KRGN VTV F
Sbjct: 622 LDMPVQPGPQPPDLLDKQLSFLTPVMMDTTPSGDSFGDVISDVPKNLSLKRGNGQVTVVF 681
Query: 685 WSGCPRNDLMTEGTFALVEILQKKD-TWVPAYDDDDFCLRFKWSRPSRLSPQSYATIEWR 744
S CPRNDL+TEGTF LVE L++KD TW P YDDDD CLRFKWSR +LS +S AT+EWR
Sbjct: 682 RSACPRNDLLTEGTFTLVERLEQKDKTWTPVYDDDDLCLRFKWSRHKKLSSRSQATVEWR 741
Query: 745 IPQTAVSGVYRIRHFGAAKIILGSIRHFT 772
IP++A GVYRI HFGAAK + GS+ HFT
Sbjct: 742 IPESASPGVYRITHFGAAKKLFGSVHHFT 770
BLAST of HG10021867 vs. ExPASy Swiss-Prot
Match:
Q304B9 (Neutral ceramidase 2 OS=Arabidopsis thaliana OX=3702 GN=NCER2 PE=3 SV=1)
HSP 1 Score: 1142.5 bits (2954), Expect = 0.0e+00
Identity = 545/750 (72.67%), Postives = 643/750 (85.73%), Query Frame = 0
Query: 22 VYMLVLLQSNRSVFSESKYLIGLGSHDITGPAADVNMMGYANAEQIASGIHFRLRARAFI 81
+ L+LL +R+V++ YLIG+GS+DITGPAADVNMMGYAN++QIASGIHFRLRARAFI
Sbjct: 11 ILFLLLLLLSRTVYA---YLIGVGSYDITGPAADVNMMGYANSDQIASGIHFRLRARAFI 70
Query: 82 VAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLYTEKNVAISGIHTHAGPGGYLQ 141
VAEPQG RVVFVNLDACMASQIVTIKVLERLKARYG+LYTEKNVAISGIHTHAGPGGYLQ
Sbjct: 71 VAEPQGNRVVFVNLDACMASQIVTIKVLERLKARYGELYTEKNVAISGIHTHAGPGGYLQ 130
Query: 142 YVVYIVTSLGFVRQSFNVLVDGIEKSIIQAHENLLPGSILINKGELIDAGVNRSPSAYLN 201
YV YIVTSLGFVRQSF+V+V+GIE+SI+QAHE+L PGS +NKG+L+DAGVNRSPS+YLN
Sbjct: 131 YVTYIVTSLGFVRQSFDVVVNGIEQSIVQAHESLRPGSAFVNKGDLLDAGVNRSPSSYLN 190
Query: 202 NPASERSRYQYDVDKEMTLLKFVDDEWGPVGSFNWFATHGTSMSRTNSLISGDNKGAAAR 261
NPA+ERS+Y+YDVDKEMTL+KFVD + GP GSFNWFATHGTSMSRTNSLISGDNKGAAAR
Sbjct: 191 NPAAERSKYKYDVDKEMTLVKFVDSQLGPTGSFNWFATHGTSMSRTNSLISGDNKGAAAR 250
Query: 262 FMEDWFEQKGIGTLYPGESEADSIPRRVSNIIPEVYENKQELLERAASFQSRPGRPATRF 321
FMEDWFE ++ + +IPRRVS I+ + N+ LL+ AA+++S G +
Sbjct: 251 FMEDWFENGQKNSV-----SSRNIPRRVSTIVSDFSRNRDRLLDIAATYKSSRGHSVDKS 310
Query: 322 LSISRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAFCIDTGLPCDFNHSTCGGKNEL 381
L + RVRN + +FVSAFCQSNCGDVSPNTLG FCIDTGLPCDFNHSTC G+NEL
Sbjct: 311 LDVKTRVRN----GSKRKFVSAFCQSNCGDVSPNTLGTFCIDTGLPCDFNHSTCNGQNEL 370
Query: 382 CYGRGPGYPDEFESTRIIGERQFRKAVDLFSKASEQLTGKVDYRHSYVDFSQLEVSLTKQ 441
CYGRGPGYPDEFESTRIIGE+QF+ AV+LF+KA+E+L GK+ Y+H+Y+DFS L+V++ K
Sbjct: 371 CYGRGPGYPDEFESTRIIGEKQFKMAVELFNKATEKLQGKIGYQHAYLDFSNLDVTVPKA 430
Query: 442 GGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGNAFWKLVRNLLKAPGNEQIACQ 501
GG +E VKTCPAAMGF FAAGTTDGPGAFDFKQGDD+GN FW+LVRN+L+ PG EQ+ CQ
Sbjct: 431 GGGSETVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNVFWRLVRNVLRTPGPEQVQCQ 490
Query: 502 SPKPILLDTGEMKTPYDWAPSILPIQILRVGQLVILGVPGEFTTMAGRRLRDAVKTVLTT 561
PKPILLDTGEMK PYDWAPSILPIQILR+GQLVIL VPGEFTTMAGRRLRDA+K+ L +
Sbjct: 491 KPKPILLDTGEMKEPYDWAPSILPIQILRIGQLVILSVPGEFTTMAGRRLRDAIKSFLIS 550
Query: 562 GAKREFNGNVHVVIAGLTNTYSQYVTTIEEYEMQRYEGASTLYGPHTLDAYVQEFKKLAQ 621
+EF+ N+HVVIAGLTNTYSQY+ T EEYE+QRYEGASTLYG HTL AY+QEFKKLA
Sbjct: 551 SDPKEFSNNMHVVIAGLTNTYSQYIATFEEYEVQRYEGASTLYGRHTLTAYIQEFKKLAT 610
Query: 622 SLIDGRPVEPGPQPPDLLARQISLLTPVIIDVTPIGVSFGDVKSDVPHNSTFKRGNLVTV 681
+L++G + GPQPPDLL +QISLL+PV++D TP+GV FGDVK+DVP STF+RG V
Sbjct: 611 ALVNGLTLPRGPQPPDLLDKQISLLSPVVVDSTPLGVKFGDVKADVPPKSTFRRGQQVNA 670
Query: 682 TFWSGCPRNDLMTEGTFALVEILQKKDTWVPAYDDDDFCLRFKWSRPSRLSPQSYATIEW 741
TFWSGCPRNDLMTEG+FA+VE L++ W P YDDDDF L+FKWSRP++LS +S ATIEW
Sbjct: 671 TFWSGCPRNDLMTEGSFAVVETLREGGKWAPVYDDDDFSLKFKWSRPAKLSSESQATIEW 730
Query: 742 RIPQTAVSGVYRIRHFGAAKIILGSIRHFT 772
R+P++AV+GVYRIRH+GA+K + GSI F+
Sbjct: 731 RVPESAVAGVYRIRHYGASKSLFGSISSFS 748
BLAST of HG10021867 vs. ExPASy Swiss-Prot
Match:
Q0JL46 (Neutral ceramidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0624000 PE=1 SV=1)
HSP 1 Score: 1132.1 bits (2927), Expect = 0.0e+00
Identity = 547/769 (71.13%), Postives = 639/769 (83.09%), Query Frame = 0
Query: 13 RSLGS--IWLCVYMLVLLQSNRSVFSESKYLIGLGSHDITGPAADVNMMGYANAEQIASG 72
R GS +WL + + ++L + V S S YL+G+GS DITGPAADVNMMGYAN EQIASG
Sbjct: 12 RGFGSSRVWLWLLLALVLLNCSLVLSASPYLVGMGSFDITGPAADVNMMGYANTEQIASG 71
Query: 73 IHFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLYTEKNVAISGI 132
IHFRL++RAFIVAEP GKRVVFVN+DACMASQIVTIKVLERLKARYGDLY E NVAISGI
Sbjct: 72 IHFRLKSRAFIVAEPNGKRVVFVNIDACMASQIVTIKVLERLKARYGDLYNENNVAISGI 131
Query: 133 HTHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQAHENLLPGSILINKGELIDA 192
HTHAGPGGYLQYVVYIVTSLGFVRQSF+V+VDGIE+SI++AH NL PG I +NKG+L+DA
Sbjct: 132 HTHAGPGGYLQYVVYIVTSLGFVRQSFDVIVDGIEQSIVEAHNNLRPGKIFVNKGDLLDA 191
Query: 193 GVNRSPSAYLNNPASERSRYQYDVDKEMTLLKFVDDEWGPVGSFNWFATHGTSMSRTNSL 252
GVNRSPSAYLNNPA ERS+Y+Y+VDKEMTL+KFVDDE GPVGSFNWFATHGTSMSRTNSL
Sbjct: 192 GVNRSPSAYLNNPAEERSKYEYNVDKEMTLIKFVDDELGPVGSFNWFATHGTSMSRTNSL 251
Query: 253 ISGDNKGAAARFMEDWFEQKGIGTLYPGESEAD--------SIPRRVSNIIPEVYENKQE 312
ISGDNKGAAARFMEDW EQ G+ + D +PRRVS IIPE E +
Sbjct: 252 ISGDNKGAAARFMEDWAEQMGLPKQSAHANSDDLRSLHKTSVLPRRVSTIIPEPNEITDD 311
Query: 313 LLERAASFQSRPGRPATRFLSISRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAFCI 372
L++ A+S+++ GR SI+RR+R+ Q ++P+FVSAFCQSNCGDVSPN LG FCI
Sbjct: 312 LIQLASSYEASGGRRLAG-SSITRRIRST--QQNKPKFVSAFCQSNCGDVSPNVLGTFCI 371
Query: 373 DTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLFSKASEQLTGKV 432
DT LPCDFNHSTC GKNELCYGRGPGYPDEFESTR+IG RQF KA DLF ASE++ GK+
Sbjct: 372 DTNLPCDFNHSTCNGKNELCYGRGPGYPDEFESTRVIGNRQFLKARDLFDSASEEIQGKI 431
Query: 433 DYRHSYVDFSQLEVSLTKQGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGNAF 492
DYRH+Y+DFS+LEV ++ G + VKTCPAAMGFAFAAGTTDGPGAFDF+QGD KGN F
Sbjct: 432 DYRHTYLDFSKLEVKVSTSAGGQQTVKTCPAAMGFAFAAGTTDGPGAFDFRQGDVKGNPF 491
Query: 493 WKLVRNLLKAPGNEQIACQSPKPILLDTGEMKTPYDWAPSILPIQILRVGQLVILGVPGE 552
WKLVRNLLK PG +Q+ C SPKPILLDTGEMK PYDWAP+ILP+Q++R+GQLVIL VPGE
Sbjct: 492 WKLVRNLLKTPGKDQVECHSPKPILLDTGEMKEPYDWAPAILPVQMIRIGQLVILCVPGE 551
Query: 553 FTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTIEEYEMQRYEGAST 612
FTTMAGRRLRDAVKTVLT+G EF+ N+HVV+AGLTN+YSQY+TT EEY++QRYEGAST
Sbjct: 552 FTTMAGRRLRDAVKTVLTSG-NSEFDKNIHVVLAGLTNSYSQYITTFEEYQIQRYEGAST 611
Query: 613 LYGPHTLDAYVQEFKKLAQSLIDGRPVEPGPQPPDLLARQISLLTPVIIDVTPIGVSFGD 672
LYGPHTL AY+QEF+KLA ++I + V QPPD+L +QI LL V+ D TP+GV FGD
Sbjct: 612 LYGPHTLSAYIQEFQKLAMAMIANKEVPTNFQPPDMLDKQIGLLPGVVFDSTPLGVKFGD 671
Query: 673 VKSDVPHNSTFKRGNLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVPAYDDDDFCLR 732
V SDVP NSTF +G+ V TF+S CPRNDL+T+GTFALVE L + WVP YDDDD+ LR
Sbjct: 672 VNSDVPGNSTFNKGSTVNATFYSACPRNDLLTDGTFALVEKLDGNNNWVPVYDDDDWSLR 731
Query: 733 FKWSRPSRLSPQSYATIEWRIPQTAVSGVYRIRHFGAAKIILGSIRHFT 772
FKWSRP+RLS +S+AT+EW +P+ A +GVYR+RHFGA+K + GS+RHFT
Sbjct: 732 FKWSRPARLSSRSFATLEWTVPEDAAAGVYRLRHFGASKPMFGSVRHFT 776
BLAST of HG10021867 vs. ExPASy Swiss-Prot
Match:
F4KHQ8 (Neutral ceramidase 3 OS=Arabidopsis thaliana OX=3702 GN=NCER3 PE=2 SV=1)
HSP 1 Score: 1075.5 bits (2780), Expect = 0.0e+00
Identity = 536/754 (71.09%), Postives = 612/754 (81.17%), Query Frame = 0
Query: 21 CVYMLVLLQSNRSVFSESKYLIGLGSHDITGPAADVNMMGYANAEQIASGIHFRLRARAF 80
C L+ L +FS+S YL+GLGS+DITGPAADVNMMGYAN EQ+ASG+HFRLRARAF
Sbjct: 10 CTLFLLFLLRLTCIFSDSDYLMGLGSYDITGPAADVNMMGYANMEQVASGVHFRLRARAF 69
Query: 81 IVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLYTEKNVAISGIHTHAGPGGYL 140
IVAEP KR+ FVNLDA MASQ+VTIKV+ERLK RYG+LYTE+NVAISG HTHAGPGGYL
Sbjct: 70 IVAEPYKKRIAFVNLDAGMASQLVTIKVIERLKQRYGELYTEENVAISGTHTHAGPGGYL 129
Query: 141 QYVVYIVTSLGFVRQSFNVLVDGIEKSIIQAHENLLPGSILINKGELIDAGVNRSPSAYL 200
QY++Y+VTSLGFV QSFN LVDGIE+SIIQAHENL PGSILINKGEL+DAGVNRSPSAYL
Sbjct: 130 QYILYLVTSLGFVHQSFNALVDGIEQSIIQAHENLRPGSILINKGELLDAGVNRSPSAYL 189
Query: 201 NNPASERSRYQYDVDKEMTLLKFVDDEWGPVGSFNWFATHGTSMSRTNSLISGDNKGAAA 260
NNPA ERS+Y+YDVDKEMTL+KFVDD+WGPV A
Sbjct: 190 NNPAHERSKYEYDVDKEMTLVKFVDDQWGPV----------------------------A 249
Query: 261 RFMEDWFEQKGIGTLYPGESEADSIPRRVSNIIPEVYENKQELLERAASFQSRPGRPATR 320
R MEDWFE++ ES PRRVS+II + Y+ Q+L+E A+S S G+ TR
Sbjct: 250 RIMEDWFERENGCRSVDVES-----PRRVSSIISDPYD--QDLMEMASSLLSTGGKTVTR 309
Query: 321 FLSISRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAFCIDTGLPCDFNHSTCGGKNE 380
S++RRVR+ R AD+P+FVSAFCQ+NCGDVSPN LGAFCIDTGLPC+FN STCGGKNE
Sbjct: 310 MSSVARRVRSRFRHADKPRFVSAFCQTNCGDVSPNVLGAFCIDTGLPCEFNQSTCGGKNE 369
Query: 381 LCYGRGPGYPDEFESTRIIGERQFRKAVDLFSKASEQLTGKVDYRHSYVDFSQLEVSLTK 440
CYGRGPGYPDEFESTRIIGERQF+KA DLF+KASE++ GKVDYRH+YVDFSQLEV++
Sbjct: 370 QCYGRGPGYPDEFESTRIIGERQFKKAADLFTKASEEIQGKVDYRHAYVDFSQLEVTING 429
Query: 441 QGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGNAFWKLVRNLLKAPGNEQIAC 500
Q G +EVVKTCPAAMGF FAAGTTDGPGAFDFKQGDD+GN FW+LVRNLLK P EQ+ C
Sbjct: 430 QNGGSEVVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNPFWRLVRNLLKNPTEEQVRC 489
Query: 501 QSPKPILLDTGEMKTPYDWAPSILPIQILRVGQLVILGVPGEFTTMAGRRLRDAVKTVLT 560
Q PKPILLDTGEMK PYDWAPSILP+QILR+GQLVIL VPGEFTTMAGRRLRDAVKTVL
Sbjct: 490 QRPKPILLDTGEMKQPYDWAPSILPVQILRIGQLVILCVPGEFTTMAGRRLRDAVKTVLK 549
Query: 561 TGAK-REFNGNVHVVIAGLTNTYSQYVTTIEEYEMQRYEGASTLYGPHTLDAYVQEFKKL 620
G+ REF+ VVIAGLTN+YSQY+ T EEY++QRYEGASTLYGPHTL Y+QEFKKL
Sbjct: 550 EGSNGREFS----VVIAGLTNSYSQYIATFEEYQVQRYEGASTLYGPHTLSGYIQEFKKL 609
Query: 621 AQSLIDGRPVEPGPQPPDLLARQISLLTPVIIDVTPIGVSFGDVKSDVPHNSTFKRG-NL 680
A L+ + +PGPQPPDLL +QISLLTPV+ D+TPIG +FGDV SDVP S F++G ++
Sbjct: 610 ANDLLSAQTTDPGPQPPDLLHKQISLLTPVVADMTPIGTAFGDVTSDVPRLSKFRKGADI 669
Query: 681 VTVTFWSGCPRNDLMTEGTFALVE-ILQKKDTWVPAYDDDDFCLRFKWSRPSRLSPQSYA 740
V V F S PRNDLMTEGTFALVE L+ ++TWVP YDDDDFCLRFKWSRP +LS QS A
Sbjct: 670 VRVQFRSANPRNDLMTEGTFALVERWLEGRETWVPVYDDDDFCLRFKWSRPFKLSTQSTA 724
Query: 741 TIEWRIPQTAVSGVYRIRHFGAAKIILGSIRHFT 772
TIEWRIP+TA GVYRI HFG+AK + SI HF+
Sbjct: 730 TIEWRIPETASPGVYRITHFGSAKTPISSIHHFS 724
BLAST of HG10021867 vs. ExPASy Swiss-Prot
Match:
Q55G11 (Neutral ceramidase B OS=Dictyostelium discoideum OX=44689 GN=dcd2B PE=3 SV=1)
HSP 1 Score: 602.1 bits (1551), Expect = 1.0e-170
Identity = 330/757 (43.59%), Postives = 447/757 (59.05%), Query Frame = 0
Query: 9 LIVRRSLGSIWLCVYMLVLLQSNRSVFSESKYLIGLGSHDITGPAADVNMMGYANAEQIA 68
+I+ S +I++ + + + Q N +S Y IG G +DITG +A+VN+MGYAN Q+
Sbjct: 15 VIILLSSNNIFIDSFKIPVNQKNVKSSGDSSYQIGAGIYDITGASAEVNLMGYANPLQVG 74
Query: 69 SGIHFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYG-DLYTEKNVAI 128
+GIHFR RARAF+ + G R V+V+ D+CM Q V I V+E L+ +G ++YTE NV +
Sbjct: 75 AGIHFRQRARAFVFVDSNGNRAVYVSTDSCMIFQEVKIHVVELLQDIFGPNVYTEANVLL 134
Query: 129 SGIHTHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQAHENLLPGSILINKGEL 188
SG HTH+GP G+ QY +Y +TSLGF +++F+ + +GI ++I++AH+++ P ++ GEL
Sbjct: 135 SGTHTHSGPAGFSQYALYGITSLGFYKKNFDTICNGIVQAIVKAHKSVQPANMFTETGEL 194
Query: 189 IDAGVNRSPSAYLNNPASERSRYQYDVDKEMTLLKFVDDEWGPVGSFNWFATHGTSMSRT 248
+ +NRSP AY NNP E++ Y +VDK MT+L+ D P + ++FA H TSM+ T
Sbjct: 195 WNTNINRSPFAYDNNPEEEKAMYDSNVDKNMTVLRIEDMNGNPFAAISFFAVHCTSMNNT 254
Query: 249 NSLISGDNKGAAARFMEDWFEQKGIGTLYPGESEADSIPRRVSNIIPEVYENKQELLERA 308
N LISGDNKG A+ E + G GT
Sbjct: 255 NHLISGDNKGYASYLWEK--QVNGPGT--------------------------------- 314
Query: 309 ASFQSRPGRPATRFLSISRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAFCIDTGLP 368
A + FV+AF QSN GDVSPNT G C D G P
Sbjct: 315 ---------------------------AGKGPFVAAFGQSNEGDVSPNTRGPTCRD-GSP 374
Query: 369 CDFNHSTCGGKNELCYGRGPGYP-DEFESTRIIGERQFRKAVDLFSKASEQLTGKVDYRH 428
CD+ STC G+NE C+ GPG D FEST+IIG QF KA++LF+ AS Q++G V YRH
Sbjct: 375 CDYKTSTCNGRNEECWSLGPGKDGDMFESTQIIGGNQFNKALELFNNASIQVSGPVQYRH 434
Query: 429 SYVDFSQLEVSLTKQGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDD--KGNAFWK 488
S+V F+ + V G TC AMG++FAAGTTDGPGAF+F Q D+ GN FW
Sbjct: 435 SWVQFTNVSVEPPYNSGVDNAT-TCRGAMGYSFAAGTTDGPGAFNFVQSDNNTSGNPFWN 494
Query: 489 LVRNLLKAPGNEQIACQSPKPILLDTGEMKTPYDWAPSILPIQILRVGQLVILGVPGEFT 548
+ + + P +QI CQSPKPILLD G M P W P ++PIQI+ +GQ+V++ VPGEFT
Sbjct: 495 FIGDFIAKPTPDQIRCQSPKPILLDVG-MVEPIPWVPDVMPIQIVTIGQIVLVAVPGEFT 554
Query: 549 TMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTIEEYEMQRYEGASTLY 608
TM+GRRLR++V+ ++ + N V+IAGL+NTYS Y+ T EEY++QRYEGAST++
Sbjct: 555 TMSGRRLRNSVREIIGESIE-----NPIVLIAGLSNTYSGYIATFEEYQVQRYEGASTVF 614
Query: 609 GPHTLDAYVQEFKKLAQSLIDGRPVEPGPQPPDLLARQISLLTPVIIDVTPIGVSFGDVK 668
GPHTL +Y+QEF KLAQS++DG V GP P +L + L PVI+D P FG+V
Sbjct: 615 GPHTLGSYMQEFGKLAQSIVDGTTVPAGPTPRNLTGHTLFFLPPVIVDAAPDFDDFGEVS 674
Query: 669 SDVPHNSTFKRGNLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVPAYDDDDFCLRFK 728
DV N + V+ F+ G PRND M E +F V++L D W DD D+ +FK
Sbjct: 675 IDV--NLNYSVNETVSCVFYGGNPRNDFMIESSFLSVDLLTGTDQWTTVLDDGDWDTKFK 697
Query: 729 WSRPSRLSPQSYATIEWRIPQTAVSGVYRIRHFGAAK 762
W S TIEW I G YRI H G AK
Sbjct: 735 WKMHD--LGFSLITIEWVIAPDTTPGTYRITHSGFAK 697
BLAST of HG10021867 vs. ExPASy TrEMBL
Match:
A0A6J1HIT9 (Neutral ceramidase OS=Cucurbita moschata OX=3662 GN=LOC111464833 PE=3 SV=1)
HSP 1 Score: 1476.8 bits (3822), Expect = 0.0e+00
Identity = 721/771 (93.51%), Postives = 747/771 (96.89%), Query Frame = 0
Query: 1 MELSSLFDLIVRRSLGSIWLCVYMLVLLQSNRSVFSESKYLIGLGSHDITGPAADVNMMG 60
M LSSLFD VRRSLGSIWLCVYMLV+LQSNR V SESKYLIGLGSHDITGPAADVNMMG
Sbjct: 1 MGLSSLFDRTVRRSLGSIWLCVYMLVILQSNRRVLSESKYLIGLGSHDITGPAADVNMMG 60
Query: 61 YANAEQIASGIHFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
YAN EQIASG+HFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY
Sbjct: 61 YANTEQIASGVHFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
Query: 121 TEKNVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQAHENLLPGSI 180
TE+NVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSF+VLVDGIEKSIIQAHENLLPGSI
Sbjct: 121 TEENVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSI 180
Query: 181 LINKGELIDAGVNRSPSAYLNNPASERSRYQYDVDKEMTLLKFVDDEWGPVGSFNWFATH 240
L+NKGELIDAGVNRSPSAYLNNPASER+RY+YDVDKEMTLLKF+DD+WGPVGSFNWFATH
Sbjct: 181 LVNKGELIDAGVNRSPSAYLNNPASERNRYKYDVDKEMTLLKFIDDQWGPVGSFNWFATH 240
Query: 241 GTSMSRTNSLISGDNKGAAARFMEDWFEQKGIGTLYPGESEADSIPRRVSNIIPEVYENK 300
GTSMSRTNSLISGDNKGAAARFMEDWF QKGIGTL G+SEADSIPRRVSNIIPE Y ++
Sbjct: 241 GTSMSRTNSLISGDNKGAAARFMEDWFVQKGIGTLQTGKSEADSIPRRVSNIIPEAYNDE 300
Query: 301 QELLERAASFQSRPGRPATRFLSISRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAF 360
QELL RAASFQSRPGRPATR LS+SRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAF
Sbjct: 301 QELLARAASFQSRPGRPATRILSVSRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAF 360
Query: 361 CIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLFSKASEQLTG 420
CIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLFSKASEQL G
Sbjct: 361 CIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLFSKASEQLKG 420
Query: 421 KVDYRHSYVDFSQLEVSLTKQGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN 480
VDYRHSYVDFSQLEVS+TK GGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN
Sbjct: 421 TVDYRHSYVDFSQLEVSVTKAGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN 480
Query: 481 AFWKLVRNLLKAPGNEQIACQSPKPILLDTGEMKTPYDWAPSILPIQILRVGQLVILGVP 540
AFWKLVRNLLK PG++QIACQSPKPILLDTGEMK PYDWAPSILPIQILR+GQLVILGVP
Sbjct: 481 AFWKLVRNLLKTPGSKQIACQSPKPILLDTGEMKIPYDWAPSILPIQILRIGQLVILGVP 540
Query: 541 GEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTIEEYEMQRYEGA 600
GEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTT EEYEMQRYEGA
Sbjct: 541 GEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTFEEYEMQRYEGA 600
Query: 601 STLYGPHTLDAYVQEFKKLAQSLIDGRPVEPGPQPPDLLARQISLLTPVIIDVTPIGVSF 660
STLYGPHTLDAY+QEF+KLAQSLIDGRPVEPGPQPPDLLA+QISLLTPV++D TP GVSF
Sbjct: 601 STLYGPHTLDAYIQEFRKLAQSLIDGRPVEPGPQPPDLLAKQISLLTPVVMDATPFGVSF 660
Query: 661 GDVKSDVPHNSTFKRGNLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVPAYDDDDFC 720
GDV+SDVP NSTFKRG+LVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVP+YDDDDFC
Sbjct: 661 GDVRSDVPLNSTFKRGDLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVPSYDDDDFC 720
Query: 721 LRFKWSRPSRLSPQSYATIEWRIPQTAVSGVYRIRHFGAAKIILGSIRHFT 772
LRFKWSRPSRLSPQS ATIEWRIPQTAVSGVYRIRHFGAAK +LGSIRHFT
Sbjct: 721 LRFKWSRPSRLSPQSLATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFT 771
BLAST of HG10021867 vs. ExPASy TrEMBL
Match:
A0A6J1I331 (Neutral ceramidase OS=Cucurbita maxima OX=3661 GN=LOC111469470 PE=3 SV=1)
HSP 1 Score: 1465.3 bits (3792), Expect = 0.0e+00
Identity = 718/771 (93.13%), Postives = 742/771 (96.24%), Query Frame = 0
Query: 1 MELSSLFDLIVRRSLGSIWLCVYMLVLLQSNRSVFSESKYLIGLGSHDITGPAADVNMMG 60
M LSSLFD VRRSLGSIWLCVYMLV+LQSNR V SESKYLIGLGSHDITGPAADVNMMG
Sbjct: 1 MGLSSLFDRTVRRSLGSIWLCVYMLVILQSNRRVLSESKYLIGLGSHDITGPAADVNMMG 60
Query: 61 YANAEQIASGIHFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
YAN EQIASG+HFRLRARAFIVAEPQGK+VVFVNLDACMASQIVTIKVLERLKARYGDLY
Sbjct: 61 YANTEQIASGVHFRLRARAFIVAEPQGKQVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
Query: 121 TEKNVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQAHENLLPGSI 180
TE+NVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSF+VLVDGIEKSIIQAHENLLPGSI
Sbjct: 121 TEENVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSI 180
Query: 181 LINKGELIDAGVNRSPSAYLNNPASERSRYQYDVDKEMTLLKFVDDEWGPVGSFNWFATH 240
L+NKGELIDA VNRSPSAYLNNPASER RY+YDVDKEMTLLKFVDD+WGPVGSFNWFATH
Sbjct: 181 LVNKGELIDASVNRSPSAYLNNPASERDRYKYDVDKEMTLLKFVDDQWGPVGSFNWFATH 240
Query: 241 GTSMSRTNSLISGDNKGAAARFMEDWFEQKGIGTLYPGESEADSIPRRVSNIIPEVYENK 300
GTSMSRTNSLISGDNKGAAARFMEDWF QKGI TL G+SEADSIPRRVSNIIPE Y+++
Sbjct: 241 GTSMSRTNSLISGDNKGAAARFMEDWFVQKGIRTLQTGKSEADSIPRRVSNIIPEAYKDE 300
Query: 301 QELLERAASFQSRPGRPATRFLSISRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAF 360
QELLERAA+FQSRPGRPATR LS+SRRVRN LRQADRPQFVSAFCQSNCGDVSPNTLGAF
Sbjct: 301 QELLERAAAFQSRPGRPATRILSVSRRVRNALRQADRPQFVSAFCQSNCGDVSPNTLGAF 360
Query: 361 CIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLFSKASEQLTG 420
CIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLFSKASEQL G
Sbjct: 361 CIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLFSKASEQLKG 420
Query: 421 KVDYRHSYVDFSQLEVSLTKQGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN 480
VDYRHSYVDFSQLEVS+TK GGAT VVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN
Sbjct: 421 TVDYRHSYVDFSQLEVSVTKPGGATAVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN 480
Query: 481 AFWKLVRNLLKAPGNEQIACQSPKPILLDTGEMKTPYDWAPSILPIQILRVGQLVILGVP 540
AFWKLVRNLLK PG+EQIACQSPKPILLDTGEMK PYDWAPSILPIQILR+GQLVILGVP
Sbjct: 481 AFWKLVRNLLKTPGSEQIACQSPKPILLDTGEMKIPYDWAPSILPIQILRIGQLVILGVP 540
Query: 541 GEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTIEEYEMQRYEGA 600
GEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTT EEYEMQRYEGA
Sbjct: 541 GEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTFEEYEMQRYEGA 600
Query: 601 STLYGPHTLDAYVQEFKKLAQSLIDGRPVEPGPQPPDLLARQISLLTPVIIDVTPIGVSF 660
STLYGPHTLDAY+QEFKKLAQSLIDGRPVEPGPQPPDLLA+QISLLTPV++D TP GVSF
Sbjct: 601 STLYGPHTLDAYIQEFKKLAQSLIDGRPVEPGPQPPDLLAKQISLLTPVVMDATPFGVSF 660
Query: 661 GDVKSDVPHNSTFKRGNLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVPAYDDDDFC 720
GDVKSDVP NSTFKRG+LVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVP+YDDDDFC
Sbjct: 661 GDVKSDVPLNSTFKRGDLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVPSYDDDDFC 720
Query: 721 LRFKWSRPSRLSPQSYATIEWRIPQTAVSGVYRIRHFGAAKIILGSIRHFT 772
LRFKW RPSRLSPQS ATIEWRIPQTAVSGVYRIRHFGAAK +L SIRHFT
Sbjct: 721 LRFKWWRPSRLSPQSLATIEWRIPQTAVSGVYRIRHFGAAKNLLRSIRHFT 771
BLAST of HG10021867 vs. ExPASy TrEMBL
Match:
A0A0A0LY63 (Neutral ceramidase OS=Cucumis sativus OX=3659 GN=Csa_1G527930 PE=3 SV=1)
HSP 1 Score: 1452.2 bits (3758), Expect = 0.0e+00
Identity = 710/771 (92.09%), Postives = 745/771 (96.63%), Query Frame = 0
Query: 1 MELSSLFDLIVRRSLGSIWLCVYMLVLLQSNRSVFSESKYLIGLGSHDITGPAADVNMMG 60
MELSSLF+L V RSLG+IWLC+YMLVL +SNRSV SESKYLIGLGSHDITGPAADVNMMG
Sbjct: 1 MELSSLFNLTVGRSLGTIWLCIYMLVLSESNRSVLSESKYLIGLGSHDITGPAADVNMMG 60
Query: 61 YANAEQIASGIHFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
YANA+QIASGIHFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY
Sbjct: 61 YANADQIASGIHFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
Query: 121 TEKNVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQAHENLLPGSI 180
TEKNVAISGIH+HAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQAHENL PGSI
Sbjct: 121 TEKNVAISGIHSHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQAHENLSPGSI 180
Query: 181 LINKGELIDAGVNRSPSAYLNNPASERSRYQYDVDKEMTLLKFVDDEWGPVGSFNWFATH 240
LINKGELIDAGVNRSPSAYLNNPASERS+Y+YDVDKEMTLLKF+DDEWGPVG+FNWFATH
Sbjct: 181 LINKGELIDAGVNRSPSAYLNNPASERSKYKYDVDKEMTLLKFIDDEWGPVGTFNWFATH 240
Query: 241 GTSMSRTNSLISGDNKGAAARFMEDWFEQKGIGTLYPGESEADSIPRRVSNIIPEVYENK 300
GTSMSRTN+LISGDNKGAAARFMEDWF+QKG GTL+ GESEADSIPRRVSNI+PEVY++K
Sbjct: 241 GTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHHGESEADSIPRRVSNIVPEVYKDK 300
Query: 301 QELLERAASFQSRPGRPATRFLSISRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAF 360
QELLE AASFQS+PGRPATR LSIS RVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAF
Sbjct: 301 QELLELAASFQSQPGRPATRVLSISSRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAF 360
Query: 361 CIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLFSKASEQLTG 420
C+DTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGE+QFRKAVDLFSKASEQLTG
Sbjct: 361 CLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGEKQFRKAVDLFSKASEQLTG 420
Query: 421 KVDYRHSYVDFSQLEVSLTKQGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN 480
KVD+RHSYVDFS LEVSL KQGG TEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN
Sbjct: 421 KVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN 480
Query: 481 AFWKLVRNLLKAPGNEQIACQSPKPILLDTGEMKTPYDWAPSILPIQILRVGQLVILGVP 540
AFWKLVRN+LKAPGNEQI+CQSPKPILLDTGEMKTPYDWAPSILPIQILR+GQLVIL VP
Sbjct: 481 AFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPYDWAPSILPIQILRIGQLVILDVP 540
Query: 541 GEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTIEEYEMQRYEGA 600
GEFTTMAGRRLRDAVKTVLTTGAK+EFN NVHVVIAGLTNTYSQYVTT EEY+MQRYEGA
Sbjct: 541 GEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAGLTNTYSQYVTTFEEYKMQRYEGA 600
Query: 601 STLYGPHTLDAYVQEFKKLAQSLIDGRPVEPGPQPPDLLARQISLLTPVIIDVTPIGVSF 660
STLYGPHTL+AY+QEFKKLAQSLIDG PV PGPQPPDLLARQISLL PVI+D+TP+GVSF
Sbjct: 601 STLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPDLLARQISLLPPVILDMTPLGVSF 660
Query: 661 GDVKSDVPHNSTFKRGNLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVPAYDDDDFC 720
GDVK DVP NS+FKRGNLV VTFW+GCPRNDLMTEGTFALVEILQ K+TWVPAYDDDDFC
Sbjct: 661 GDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQ-KNTWVPAYDDDDFC 720
Query: 721 LRFKWSRPSRLSPQSYATIEWRIPQTAVSGVYRIRHFGAAKIILGSIRHFT 772
LRFKWSRP+ LS QSYATIEWRIPQTAVSGVYRIRHFGAAK +LGSIRHFT
Sbjct: 721 LRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFT 770
BLAST of HG10021867 vs. ExPASy TrEMBL
Match:
A0A6J1DAR9 (Neutral ceramidase OS=Momordica charantia OX=3673 GN=LOC111018960 PE=3 SV=1)
HSP 1 Score: 1446.8 bits (3744), Expect = 0.0e+00
Identity = 706/771 (91.57%), Postives = 736/771 (95.46%), Query Frame = 0
Query: 1 MELSSLFDLIVRRSLGSIWLCVYMLVLLQSNRSVFSESKYLIGLGSHDITGPAADVNMMG 60
M LSSL DL V RSL ++WL + MLVLLQSN V SESKYLIGLGS+DITGPAADVNMMG
Sbjct: 1 MGLSSLVDLTVGRSLATLWLWICMLVLLQSNGRVLSESKYLIGLGSYDITGPAADVNMMG 60
Query: 61 YANAEQIASGIHFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
YAN EQIASG+HFRLRARAFIVAEPQG RVVFVNLDACMASQ+VTIKVLERLKARYGDLY
Sbjct: 61 YANIEQIASGVHFRLRARAFIVAEPQGNRVVFVNLDACMASQLVTIKVLERLKARYGDLY 120
Query: 121 TEKNVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQAHENLLPGSI 180
TEKNVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSF+VLVDGIEKSIIQAHENLLPGS+
Sbjct: 121 TEKNVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSV 180
Query: 181 LINKGELIDAGVNRSPSAYLNNPASERSRYQYDVDKEMTLLKFVDDEWGPVGSFNWFATH 240
LIN GELIDAGVNRSPSAYLNNPASERSRY+YDVDKEMTLLKFVDDEWGPVGSFNWFATH
Sbjct: 181 LINTGELIDAGVNRSPSAYLNNPASERSRYKYDVDKEMTLLKFVDDEWGPVGSFNWFATH 240
Query: 241 GTSMSRTNSLISGDNKGAAARFMEDWFEQKGIGTLYPGESEADSIPRRVSNIIPEVYENK 300
GTSMSRTNSLISGDNKGAAARFMEDWF+QKGIG+L+PG+S ADSIPRRVSNIIPE Y+NK
Sbjct: 241 GTSMSRTNSLISGDNKGAAARFMEDWFDQKGIGSLHPGQSVADSIPRRVSNIIPEAYKNK 300
Query: 301 QELLERAASFQSRPGRPATRFLSISRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAF 360
QELLERAASFQSRPGRPATR LS++RRVRNVLRQADRPQFVSAFCQSNCGDVSPN LGAF
Sbjct: 301 QELLERAASFQSRPGRPATRILSVARRVRNVLRQADRPQFVSAFCQSNCGDVSPNVLGAF 360
Query: 361 CIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLFSKASEQLTG 420
CIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFES RIIGERQFRKAVDLF+KASE+LTG
Sbjct: 361 CIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESARIIGERQFRKAVDLFNKASEKLTG 420
Query: 421 KVDYRHSYVDFSQLEVSLTKQGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN 480
KVDYRHSYVDFSQLEVSL KQGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN
Sbjct: 421 KVDYRHSYVDFSQLEVSLLKQGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN 480
Query: 481 AFWKLVRNLLKAPGNEQIACQSPKPILLDTGEMKTPYDWAPSILPIQILRVGQLVILGVP 540
FWKLVRNLL+APG +Q+ CQSPKPILLDTGEMK PYDWAPSILPIQILR+GQLVILGVP
Sbjct: 481 PFWKLVRNLLRAPGEQQVTCQSPKPILLDTGEMKAPYDWAPSILPIQILRIGQLVILGVP 540
Query: 541 GEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTIEEYEMQRYEGA 600
GEFTTMAGRRLR+AVKTVLTTGAKREFNGNVH+VIAGLTNTYSQYVTT EEYEMQRYEGA
Sbjct: 541 GEFTTMAGRRLRNAVKTVLTTGAKREFNGNVHIVIAGLTNTYSQYVTTFEEYEMQRYEGA 600
Query: 601 STLYGPHTLDAYVQEFKKLAQSLIDGRPVEPGPQPPDLLARQISLLTPVIIDVTPIGVSF 660
STLYGPHTLDAY+QEFKKLAQSLIDGRPVEPGPQPPDLL+RQISLL PVI+D TP+GVSF
Sbjct: 601 STLYGPHTLDAYIQEFKKLAQSLIDGRPVEPGPQPPDLLSRQISLLPPVIMDATPLGVSF 660
Query: 661 GDVKSDVPHNSTFKRGNLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVPAYDDDDFC 720
GDVKSDVP NSTFKRGNLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVP YDDDDFC
Sbjct: 661 GDVKSDVPLNSTFKRGNLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVPVYDDDDFC 720
Query: 721 LRFKWSRPSRLSPQSYATIEWRIPQTAVSGVYRIRHFGAAKIILGSIRHFT 772
L FKW RP R S QSYATIEWRIPQ+AVSGVYRIRHFGAAK + GSIRHFT
Sbjct: 721 LFFKWLRPFRYSTQSYATIEWRIPQSAVSGVYRIRHFGAAKSLFGSIRHFT 771
BLAST of HG10021867 vs. ExPASy TrEMBL
Match:
A0A1S3C5F7 (Neutral ceramidase OS=Cucumis melo OX=3656 GN=LOC103497106 PE=3 SV=1)
HSP 1 Score: 1444.5 bits (3738), Expect = 0.0e+00
Identity = 707/771 (91.70%), Postives = 738/771 (95.72%), Query Frame = 0
Query: 1 MELSSLFDLIVRRSLGSIWLCVYMLVLLQSNRSVFSESKYLIGLGSHDITGPAADVNMMG 60
MELSSLF+L VRRSLG+IWLC+YMLVL QS RSV SESKYLIGLGSHDITGPAADVNMMG
Sbjct: 1 MELSSLFNLTVRRSLGTIWLCIYMLVLSQSTRSVLSESKYLIGLGSHDITGPAADVNMMG 60
Query: 61 YANAEQIASGIHFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
YANAEQIASG+HFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY
Sbjct: 61 YANAEQIASGVHFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
Query: 121 TEKNVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQAHENLLPGSI 180
TEKNVAISGIH+HAGPGGYLQY+VYIVTSLGFVRQSF+VLVDGIEKSIIQAHENL PGSI
Sbjct: 121 TEKNVAISGIHSHAGPGGYLQYIVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLSPGSI 180
Query: 181 LINKGELIDAGVNRSPSAYLNNPASERSRYQYDVDKEMTLLKFVDDEWGPVGSFNWFATH 240
LINKGELIDAGVNRSPSAYLNNPASE S+Y+YDVDKEMTLLKF+DDEWGPVG+FNWFATH
Sbjct: 181 LINKGELIDAGVNRSPSAYLNNPASECSKYKYDVDKEMTLLKFIDDEWGPVGTFNWFATH 240
Query: 241 GTSMSRTNSLISGDNKGAAARFMEDWFEQKGIGTLYPGESEADSIPRRVSNIIPEVYENK 300
GTSMSRTNSLISGDNKGAAARFMEDWFEQKG GTL+PGESEADSIPRRVSNIIPEVY++K
Sbjct: 241 GTSMSRTNSLISGDNKGAAARFMEDWFEQKGTGTLHPGESEADSIPRRVSNIIPEVYKDK 300
Query: 301 QELLERAASFQSRPGRPATRFLSISRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAF 360
QELLERAASFQS+PGRPATR LS+S RVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAF
Sbjct: 301 QELLERAASFQSQPGRPATRALSVSSRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAF 360
Query: 361 CIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLFSKASEQLTG 420
C+DT LPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLF KASE LTG
Sbjct: 361 CLDTELPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLFGKASEHLTG 420
Query: 421 KVDYRHSYVDFSQLEVSLTKQGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN 480
KVD+RHSYVDFS LEVSLTKQGGA EVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN
Sbjct: 421 KVDFRHSYVDFSHLEVSLTKQGGAAEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN 480
Query: 481 AFWKLVRNLLKAPGNEQIACQSPKPILLDTGEMKTPYDWAPSILPIQILRVGQLVILGVP 540
FWKLVRNLLKAPGNEQI+CQSPKPILLDTGEMK PYDWAP+ILPIQILR+GQLVIL VP
Sbjct: 481 PFWKLVRNLLKAPGNEQISCQSPKPILLDTGEMKIPYDWAPTILPIQILRIGQLVILSVP 540
Query: 541 GEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTIEEYEMQRYEGA 600
GEFTTMAGRRLRDAVKTVLTTGA +EFN NVHVVIAGLTNTYSQYVTT EEY+MQRYEGA
Sbjct: 541 GEFTTMAGRRLRDAVKTVLTTGANKEFNSNVHVVIAGLTNTYSQYVTTFEEYKMQRYEGA 600
Query: 601 STLYGPHTLDAYVQEFKKLAQSLIDGRPVEPGPQPPDLLARQISLLTPVIIDVTPIGVSF 660
STLYGPHTL+AY+QEFKKLAQSLIDGRPV PGPQPPDLL RQISLL PVI+D+TP GVSF
Sbjct: 601 STLYGPHTLEAYIQEFKKLAQSLIDGRPVVPGPQPPDLLDRQISLLPPVILDMTPPGVSF 660
Query: 661 GDVKSDVPHNSTFKRGNLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVPAYDDDDFC 720
GDVKSDVP NS+FKRGNLV VTFW+GCPRNDLMTEGTFALVEILQ K+TWVPAYDDDDFC
Sbjct: 661 GDVKSDVPRNSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQ-KNTWVPAYDDDDFC 720
Query: 721 LRFKWSRPSRLSPQSYATIEWRIPQTAVSGVYRIRHFGAAKIILGSIRHFT 772
LRFKWSRP+ LS QSYATIEWRIPQT VSGVYRIRHFGAAK GSIRHFT
Sbjct: 721 LRFKWSRPAPLSAQSYATIEWRIPQTVVSGVYRIRHFGAAKSFFGSIRHFT 770
BLAST of HG10021867 vs. TAIR 10
Match:
AT1G07380.1 (Neutral/alkaline non-lysosomal ceramidase )
HSP 1 Score: 1169.8 bits (3025), Expect = 0.0e+00
Identity = 563/749 (75.17%), Postives = 640/749 (85.45%), Query Frame = 0
Query: 25 LVLLQSNRSVFSESKYLIGLGSHDITGPAADVNMMGYANAEQIASGIHFRLRARAFIVAE 84
++ L + V+S S+YLIGLGS+DITGPAADVNMMGYAN EQ+ASGIHFRLRAR FIV+E
Sbjct: 22 VLFLLTGGGVYSHSEYLIGLGSYDITGPAADVNMMGYANMEQVASGIHFRLRARTFIVSE 81
Query: 85 PQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLYTEKNVAISGIHTHAGPGGYLQYVV 144
PQGKRVVFVNLDACMASQIV +KV+ERLKARYGDLYTE+NV ISGIHTHAGPGGYLQYVV
Sbjct: 82 PQGKRVVFVNLDACMASQIVKLKVIERLKARYGDLYTEQNVGISGIHTHAGPGGYLQYVV 141
Query: 145 YIVTSLGFVRQSFNVLVDGIEKSIIQAHENLLPGSILINKGELIDAGVNRSPSAYLNNPA 204
YIVTSLGFVRQSF+ LVDGIE SIIQAHENL PGSI +N GEL+DAGVNRSPSAYLNNP+
Sbjct: 142 YIVTSLGFVRQSFDALVDGIENSIIQAHENLRPGSIFLNNGELLDAGVNRSPSAYLNNPS 201
Query: 205 SERSRYQYDVDKEMTLLKFVDDEWGPVGSFNWFATHGTSMSRTNSLISGDNKGAAARFME 264
ERS+++Y+VDKEMTLLKFVDD+WGPVGSFNWFATHGTSMSRTNSLISGDNKGAA+RFME
Sbjct: 202 KERSKHKYNVDKEMTLLKFVDDQWGPVGSFNWFATHGTSMSRTNSLISGDNKGAASRFME 261
Query: 265 DWFEQKGIGTLYPGESEADSIPRRVSNIIPEVYENKQELLERAASFQSRPGRPATRFLSI 324
DW+EQ Y E +D IPRRVS++I ++ ELLE A+ F+S+PG+P TR S
Sbjct: 262 DWYEQNTAERSYSEEFISDEIPRRVSSLIENHQDSHHELLELASYFESQPGKPVTRISSS 321
Query: 325 SRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAFCIDTGLPCDFNHSTCGGKNELCYG 384
+RRVR+ LR+AD+P FVSAFCQ+NCGDVSPN LGAFC+DTGLPCDFNHSTCGGKNE+CYG
Sbjct: 322 ARRVRSALRKADKPGFVSAFCQTNCGDVSPNVLGAFCLDTGLPCDFNHSTCGGKNEMCYG 381
Query: 385 RGPGYPDEFESTRIIGERQFRKAVDLFSKASEQLTGKVDYRHSYVDFSQLEVSLTKQGGA 444
RGPGYPDEFESTRIIGERQF+ A++LF+KASEQL GKVDYRH YVDFSQL V+L K+ G
Sbjct: 382 RGPGYPDEFESTRIIGERQFKMALELFNKASEQLQGKVDYRHVYVDFSQLNVTLPKKDGK 441
Query: 445 TEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGNAFWKLVRNLLKAPGNEQIACQSPK 504
+EVVKTCPAAMGFAFAAGTTDGPGAFDF QGDDKGN FW+LVRN+LK P +QI C PK
Sbjct: 442 SEVVKTCPAAMGFAFAAGTTDGPGAFDFTQGDDKGNPFWRLVRNVLKTPDKKQIDCHYPK 501
Query: 505 PILLDTGEMKTPYDWAPSILPIQILRVGQLVILGVPGEFTTMAGRRLRDAVKTVLTTGAK 564
PILLDTGEM PYDWAPSIL +Q+LR+GQL IL VPGEFTTMAGRRLR AVKT L
Sbjct: 502 PILLDTGEMTKPYDWAPSILSLQVLRIGQLFILSVPGEFTTMAGRRLRYAVKTQLKNSGN 561
Query: 565 REFNGNVHVVIAGLTNTYSQYVTTIEEYEMQRYEGASTLYGPHTLDAYVQEFKKLAQSLI 624
++ +G +HVVIAGL N YSQYVTT EEY++QRYEGASTLYGPHTL Y+QEFKKL++SL+
Sbjct: 562 KDLSGEIHVVIAGLANGYSQYVTTFEEYQVQRYEGASTLYGPHTLSGYIQEFKKLSKSLV 621
Query: 625 DGRPVEPGPQPPDLLARQISLLTPVIIDVTPIGVSFGDVKSDVPHNSTFKRGN-LVTVTF 684
PV+PGPQPPDLL +Q+S LTPV++D TP G SFGDV SDVP N + KRGN VTV F
Sbjct: 622 LDMPVQPGPQPPDLLDKQLSFLTPVMMDTTPSGDSFGDVISDVPKNLSLKRGNGQVTVVF 681
Query: 685 WSGCPRNDLMTEGTFALVEILQKKD-TWVPAYDDDDFCLRFKWSRPSRLSPQSYATIEWR 744
S CPRNDL+TEGTF LVE L++KD TW P YDDDD CLRFKWSR +LS +S AT+EWR
Sbjct: 682 RSACPRNDLLTEGTFTLVERLEQKDKTWTPVYDDDDLCLRFKWSRHKKLSSRSQATVEWR 741
Query: 745 IPQTAVSGVYRIRHFGAAKIILGSIRHFT 772
IP++A GVYRI HFGAAK + GS+ HFT
Sbjct: 742 IPESASPGVYRITHFGAAKKLFGSVHHFT 770
BLAST of HG10021867 vs. TAIR 10
Match:
AT2G38010.1 (Neutral/alkaline non-lysosomal ceramidase )
HSP 1 Score: 1142.5 bits (2954), Expect = 0.0e+00
Identity = 545/750 (72.67%), Postives = 643/750 (85.73%), Query Frame = 0
Query: 22 VYMLVLLQSNRSVFSESKYLIGLGSHDITGPAADVNMMGYANAEQIASGIHFRLRARAFI 81
+ L+LL +R+V++ YLIG+GS+DITGPAADVNMMGYAN++QIASGIHFRLRARAFI
Sbjct: 11 ILFLLLLLLSRTVYA---YLIGVGSYDITGPAADVNMMGYANSDQIASGIHFRLRARAFI 70
Query: 82 VAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLYTEKNVAISGIHTHAGPGGYLQ 141
VAEPQG RVVFVNLDACMASQIVTIKVLERLKARYG+LYTEKNVAISGIHTHAGPGGYLQ
Sbjct: 71 VAEPQGNRVVFVNLDACMASQIVTIKVLERLKARYGELYTEKNVAISGIHTHAGPGGYLQ 130
Query: 142 YVVYIVTSLGFVRQSFNVLVDGIEKSIIQAHENLLPGSILINKGELIDAGVNRSPSAYLN 201
YV YIVTSLGFVRQSF+V+V+GIE+SI+QAHE+L PGS +NKG+L+DAGVNRSPS+YLN
Sbjct: 131 YVTYIVTSLGFVRQSFDVVVNGIEQSIVQAHESLRPGSAFVNKGDLLDAGVNRSPSSYLN 190
Query: 202 NPASERSRYQYDVDKEMTLLKFVDDEWGPVGSFNWFATHGTSMSRTNSLISGDNKGAAAR 261
NPA+ERS+Y+YDVDKEMTL+KFVD + GP GSFNWFATHGTSMSRTNSLISGDNKGAAAR
Sbjct: 191 NPAAERSKYKYDVDKEMTLVKFVDSQLGPTGSFNWFATHGTSMSRTNSLISGDNKGAAAR 250
Query: 262 FMEDWFEQKGIGTLYPGESEADSIPRRVSNIIPEVYENKQELLERAASFQSRPGRPATRF 321
FMEDWFE ++ + +IPRRVS I+ + N+ LL+ AA+++S G +
Sbjct: 251 FMEDWFENGQKNSV-----SSRNIPRRVSTIVSDFSRNRDRLLDIAATYKSSRGHSVDKS 310
Query: 322 LSISRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAFCIDTGLPCDFNHSTCGGKNEL 381
L + RVRN + +FVSAFCQSNCGDVSPNTLG FCIDTGLPCDFNHSTC G+NEL
Sbjct: 311 LDVKTRVRN----GSKRKFVSAFCQSNCGDVSPNTLGTFCIDTGLPCDFNHSTCNGQNEL 370
Query: 382 CYGRGPGYPDEFESTRIIGERQFRKAVDLFSKASEQLTGKVDYRHSYVDFSQLEVSLTKQ 441
CYGRGPGYPDEFESTRIIGE+QF+ AV+LF+KA+E+L GK+ Y+H+Y+DFS L+V++ K
Sbjct: 371 CYGRGPGYPDEFESTRIIGEKQFKMAVELFNKATEKLQGKIGYQHAYLDFSNLDVTVPKA 430
Query: 442 GGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGNAFWKLVRNLLKAPGNEQIACQ 501
GG +E VKTCPAAMGF FAAGTTDGPGAFDFKQGDD+GN FW+LVRN+L+ PG EQ+ CQ
Sbjct: 431 GGGSETVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNVFWRLVRNVLRTPGPEQVQCQ 490
Query: 502 SPKPILLDTGEMKTPYDWAPSILPIQILRVGQLVILGVPGEFTTMAGRRLRDAVKTVLTT 561
PKPILLDTGEMK PYDWAPSILPIQILR+GQLVIL VPGEFTTMAGRRLRDA+K+ L +
Sbjct: 491 KPKPILLDTGEMKEPYDWAPSILPIQILRIGQLVILSVPGEFTTMAGRRLRDAIKSFLIS 550
Query: 562 GAKREFNGNVHVVIAGLTNTYSQYVTTIEEYEMQRYEGASTLYGPHTLDAYVQEFKKLAQ 621
+EF+ N+HVVIAGLTNTYSQY+ T EEYE+QRYEGASTLYG HTL AY+QEFKKLA
Sbjct: 551 SDPKEFSNNMHVVIAGLTNTYSQYIATFEEYEVQRYEGASTLYGRHTLTAYIQEFKKLAT 610
Query: 622 SLIDGRPVEPGPQPPDLLARQISLLTPVIIDVTPIGVSFGDVKSDVPHNSTFKRGNLVTV 681
+L++G + GPQPPDLL +QISLL+PV++D TP+GV FGDVK+DVP STF+RG V
Sbjct: 611 ALVNGLTLPRGPQPPDLLDKQISLLSPVVVDSTPLGVKFGDVKADVPPKSTFRRGQQVNA 670
Query: 682 TFWSGCPRNDLMTEGTFALVEILQKKDTWVPAYDDDDFCLRFKWSRPSRLSPQSYATIEW 741
TFWSGCPRNDLMTEG+FA+VE L++ W P YDDDDF L+FKWSRP++LS +S ATIEW
Sbjct: 671 TFWSGCPRNDLMTEGSFAVVETLREGGKWAPVYDDDDFSLKFKWSRPAKLSSESQATIEW 730
Query: 742 RIPQTAVSGVYRIRHFGAAKIILGSIRHFT 772
R+P++AV+GVYRIRH+GA+K + GSI F+
Sbjct: 731 RVPESAVAGVYRIRHYGASKSLFGSISSFS 748
BLAST of HG10021867 vs. TAIR 10
Match:
AT2G38010.2 (Neutral/alkaline non-lysosomal ceramidase )
HSP 1 Score: 1125.5 bits (2910), Expect = 0.0e+00
Identity = 545/785 (69.43%), Postives = 644/785 (82.04%), Query Frame = 0
Query: 22 VYMLVLLQSNRSVFSESKYLIGLGSHDITGPAADVNMMGYANAEQIASGIHFRLRARAFI 81
+ L+LL +R+V++ YLIG+GS+DITGPAADVNMMGYAN++QIASGIHFRLRARAFI
Sbjct: 11 ILFLLLLLLSRTVYA---YLIGVGSYDITGPAADVNMMGYANSDQIASGIHFRLRARAFI 70
Query: 82 VAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLYTEKNVAISGIHTHAGPGGYLQ 141
VAEPQG RVVFVNLDACMASQIVTIKVLERLKARYG+LYTEKNVAISGIHTHAGPGGYLQ
Sbjct: 71 VAEPQGNRVVFVNLDACMASQIVTIKVLERLKARYGELYTEKNVAISGIHTHAGPGGYLQ 130
Query: 142 YVVYIVTSLGFVRQSFNVLVDGIEKSIIQAHENLLPGSILINKGELIDAGVNRSPSAYLN 201
YV YIVTSLGFVRQSF+V+V+GIE+SI+QAHE+L PGS +NKG+L+DAGVNRSPS+YLN
Sbjct: 131 YVTYIVTSLGFVRQSFDVVVNGIEQSIVQAHESLRPGSAFVNKGDLLDAGVNRSPSSYLN 190
Query: 202 NPASERSRYQYDVDKEMTLLKFVDDEWGPVGSFNWFATHGTSMSRTNSLISGDNKGAAAR 261
NPA+ERS+Y+YDVDKEMTL+KFVD + GP GSFNWFATHGTSMSRTNSLISGDNKGAAAR
Sbjct: 191 NPAAERSKYKYDVDKEMTLVKFVDSQLGPTGSFNWFATHGTSMSRTNSLISGDNKGAAAR 250
Query: 262 FMEDWFEQKGIGTLYPGESEADSIPRRVSNIIPEVYENKQ-------------------- 321
FMEDWFE ++ + +IPRRVS I+ + N++
Sbjct: 251 FMEDWFENGQKNSV-----SSRNIPRRVSTIVSDFSRNRESYPFLELPNVAILYIAVAER 310
Query: 322 ---------------ELLERAASFQSRPGRPATRFLSISRRVRNVLRQADRPQFVSAFCQ 381
LL+ AA+++S G + L + RVRN + +FVSAFCQ
Sbjct: 311 LMWKVMVLFYFVSESRLLDIAATYKSSRGHSVDKSLDVKTRVRN----GSKRKFVSAFCQ 370
Query: 382 SNCGDVSPNTLGAFCIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRK 441
SNCGDVSPNTLG FCIDTGLPCDFNHSTC G+NELCYGRGPGYPDEFESTRIIGE+QF+
Sbjct: 371 SNCGDVSPNTLGTFCIDTGLPCDFNHSTCNGQNELCYGRGPGYPDEFESTRIIGEKQFKM 430
Query: 442 AVDLFSKASEQLTGKVDYRHSYVDFSQLEVSLTKQGGATEVVKTCPAAMGFAFAAGTTDG 501
AV+LF+KA+E+L GK+ Y+H+Y+DFS L+V++ K GG +E VKTCPAAMGF FAAGTTDG
Sbjct: 431 AVELFNKATEKLQGKIGYQHAYLDFSNLDVTVPKAGGGSETVKTCPAAMGFGFAAGTTDG 490
Query: 502 PGAFDFKQGDDKGNAFWKLVRNLLKAPGNEQIACQSPKPILLDTGEMKTPYDWAPSILPI 561
PGAFDFKQGDD+GN FW+LVRN+L+ PG EQ+ CQ PKPILLDTGEMK PYDWAPSILPI
Sbjct: 491 PGAFDFKQGDDQGNVFWRLVRNVLRTPGPEQVQCQKPKPILLDTGEMKEPYDWAPSILPI 550
Query: 562 QILRVGQLVILGVPGEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYV 621
QILR+GQLVIL VPGEFTTMAGRRLRDA+K+ L + +EF+ N+HVVIAGLTNTYSQY+
Sbjct: 551 QILRIGQLVILSVPGEFTTMAGRRLRDAIKSFLISSDPKEFSNNMHVVIAGLTNTYSQYI 610
Query: 622 TTIEEYEMQRYEGASTLYGPHTLDAYVQEFKKLAQSLIDGRPVEPGPQPPDLLARQISLL 681
T EEYE+QRYEGASTLYG HTL AY+QEFKKLA +L++G + GPQPPDLL +QISLL
Sbjct: 611 ATFEEYEVQRYEGASTLYGRHTLTAYIQEFKKLATALVNGLTLPRGPQPPDLLDKQISLL 670
Query: 682 TPVIIDVTPIGVSFGDVKSDVPHNSTFKRGNLVTVTFWSGCPRNDLMTEGTFALVEILQK 741
+PV++D TP+GV FGDVK+DVP STF+RG V TFWSGCPRNDLMTEG+FA+VE L++
Sbjct: 671 SPVVVDSTPLGVKFGDVKADVPPKSTFRRGQQVNATFWSGCPRNDLMTEGSFAVVETLRE 730
Query: 742 KDTWVPAYDDDDFCLRFKWSRPSRLSPQSYATIEWRIPQTAVSGVYRIRHFGAAKIILGS 772
W P YDDDDF L+FKWSRP++LS +S ATIEWR+P++AV+GVYRIRH+GA+K + GS
Sbjct: 731 GGKWAPVYDDDDFSLKFKWSRPAKLSSESQATIEWRVPESAVAGVYRIRHYGASKSLFGS 783
BLAST of HG10021867 vs. TAIR 10
Match:
AT5G58980.1 (Neutral/alkaline non-lysosomal ceramidase )
HSP 1 Score: 1075.5 bits (2780), Expect = 0.0e+00
Identity = 536/754 (71.09%), Postives = 612/754 (81.17%), Query Frame = 0
Query: 21 CVYMLVLLQSNRSVFSESKYLIGLGSHDITGPAADVNMMGYANAEQIASGIHFRLRARAF 80
C L+ L +FS+S YL+GLGS+DITGPAADVNMMGYAN EQ+ASG+HFRLRARAF
Sbjct: 10 CTLFLLFLLRLTCIFSDSDYLMGLGSYDITGPAADVNMMGYANMEQVASGVHFRLRARAF 69
Query: 81 IVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLYTEKNVAISGIHTHAGPGGYL 140
IVAEP KR+ FVNLDA MASQ+VTIKV+ERLK RYG+LYTE+NVAISG HTHAGPGGYL
Sbjct: 70 IVAEPYKKRIAFVNLDAGMASQLVTIKVIERLKQRYGELYTEENVAISGTHTHAGPGGYL 129
Query: 141 QYVVYIVTSLGFVRQSFNVLVDGIEKSIIQAHENLLPGSILINKGELIDAGVNRSPSAYL 200
QY++Y+VTSLGFV QSFN LVDGIE+SIIQAHENL PGSILINKGEL+DAGVNRSPSAYL
Sbjct: 130 QYILYLVTSLGFVHQSFNALVDGIEQSIIQAHENLRPGSILINKGELLDAGVNRSPSAYL 189
Query: 201 NNPASERSRYQYDVDKEMTLLKFVDDEWGPVGSFNWFATHGTSMSRTNSLISGDNKGAAA 260
NNPA ERS+Y+YDVDKEMTL+KFVDD+WGPV A
Sbjct: 190 NNPAHERSKYEYDVDKEMTLVKFVDDQWGPV----------------------------A 249
Query: 261 RFMEDWFEQKGIGTLYPGESEADSIPRRVSNIIPEVYENKQELLERAASFQSRPGRPATR 320
R MEDWFE++ ES PRRVS+II + Y+ Q+L+E A+S S G+ TR
Sbjct: 250 RIMEDWFERENGCRSVDVES-----PRRVSSIISDPYD--QDLMEMASSLLSTGGKTVTR 309
Query: 321 FLSISRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAFCIDTGLPCDFNHSTCGGKNE 380
S++RRVR+ R AD+P+FVSAFCQ+NCGDVSPN LGAFCIDTGLPC+FN STCGGKNE
Sbjct: 310 MSSVARRVRSRFRHADKPRFVSAFCQTNCGDVSPNVLGAFCIDTGLPCEFNQSTCGGKNE 369
Query: 381 LCYGRGPGYPDEFESTRIIGERQFRKAVDLFSKASEQLTGKVDYRHSYVDFSQLEVSLTK 440
CYGRGPGYPDEFESTRIIGERQF+KA DLF+KASE++ GKVDYRH+YVDFSQLEV++
Sbjct: 370 QCYGRGPGYPDEFESTRIIGERQFKKAADLFTKASEEIQGKVDYRHAYVDFSQLEVTING 429
Query: 441 QGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGNAFWKLVRNLLKAPGNEQIAC 500
Q G +EVVKTCPAAMGF FAAGTTDGPGAFDFKQGDD+GN FW+LVRNLLK P EQ+ C
Sbjct: 430 QNGGSEVVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNPFWRLVRNLLKNPTEEQVRC 489
Query: 501 QSPKPILLDTGEMKTPYDWAPSILPIQILRVGQLVILGVPGEFTTMAGRRLRDAVKTVLT 560
Q PKPILLDTGEMK PYDWAPSILP+QILR+GQLVIL VPGEFTTMAGRRLRDAVKTVL
Sbjct: 490 QRPKPILLDTGEMKQPYDWAPSILPVQILRIGQLVILCVPGEFTTMAGRRLRDAVKTVLK 549
Query: 561 TGAK-REFNGNVHVVIAGLTNTYSQYVTTIEEYEMQRYEGASTLYGPHTLDAYVQEFKKL 620
G+ REF+ VVIAGLTN+YSQY+ T EEY++QRYEGASTLYGPHTL Y+QEFKKL
Sbjct: 550 EGSNGREFS----VVIAGLTNSYSQYIATFEEYQVQRYEGASTLYGPHTLSGYIQEFKKL 609
Query: 621 AQSLIDGRPVEPGPQPPDLLARQISLLTPVIIDVTPIGVSFGDVKSDVPHNSTFKRG-NL 680
A L+ + +PGPQPPDLL +QISLLTPV+ D+TPIG +FGDV SDVP S F++G ++
Sbjct: 610 ANDLLSAQTTDPGPQPPDLLHKQISLLTPVVADMTPIGTAFGDVTSDVPRLSKFRKGADI 669
Query: 681 VTVTFWSGCPRNDLMTEGTFALVE-ILQKKDTWVPAYDDDDFCLRFKWSRPSRLSPQSYA 740
V V F S PRNDLMTEGTFALVE L+ ++TWVP YDDDDFCLRFKWSRP +LS QS A
Sbjct: 670 VRVQFRSANPRNDLMTEGTFALVERWLEGRETWVPVYDDDDFCLRFKWSRPFKLSTQSTA 724
Query: 741 TIEWRIPQTAVSGVYRIRHFGAAKIILGSIRHFT 772
TIEWRIP+TA GVYRI HFG+AK + SI HF+
Sbjct: 730 TIEWRIPETASPGVYRITHFGSAKTPISSIHHFS 724
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
F4HQM3 | 0.0e+00 | 75.17 | Neutral ceramidase 1 OS=Arabidopsis thaliana OX=3702 GN=NCER1 PE=2 SV=1 | [more] |
Q304B9 | 0.0e+00 | 72.67 | Neutral ceramidase 2 OS=Arabidopsis thaliana OX=3702 GN=NCER2 PE=3 SV=1 | [more] |
Q0JL46 | 0.0e+00 | 71.13 | Neutral ceramidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0624000 PE=1... | [more] |
F4KHQ8 | 0.0e+00 | 71.09 | Neutral ceramidase 3 OS=Arabidopsis thaliana OX=3702 GN=NCER3 PE=2 SV=1 | [more] |
Q55G11 | 1.0e-170 | 43.59 | Neutral ceramidase B OS=Dictyostelium discoideum OX=44689 GN=dcd2B PE=3 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1HIT9 | 0.0e+00 | 93.51 | Neutral ceramidase OS=Cucurbita moschata OX=3662 GN=LOC111464833 PE=3 SV=1 | [more] |
A0A6J1I331 | 0.0e+00 | 93.13 | Neutral ceramidase OS=Cucurbita maxima OX=3661 GN=LOC111469470 PE=3 SV=1 | [more] |
A0A0A0LY63 | 0.0e+00 | 92.09 | Neutral ceramidase OS=Cucumis sativus OX=3659 GN=Csa_1G527930 PE=3 SV=1 | [more] |
A0A6J1DAR9 | 0.0e+00 | 91.57 | Neutral ceramidase OS=Momordica charantia OX=3673 GN=LOC111018960 PE=3 SV=1 | [more] |
A0A1S3C5F7 | 0.0e+00 | 91.70 | Neutral ceramidase OS=Cucumis melo OX=3656 GN=LOC103497106 PE=3 SV=1 | [more] |