HG10021216 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10021216 vs. NCBI nr
Match: XP_038893889.1 (callose synthase 11-like [Benincasa hispida])

HSP 1 Score: 3436.4 bits (8909), Expect = 0.0e+00
Identity = 1714/1769 (96.89%), Postives = 1741/1769 (98.42%), Query Frame = 0

Query: 1    MNMRQRPQPAGRGGFPNPPPPVEPYNIIPIHDLLTDHPSLQSPEVRAAAAALRTVGELRR 60
            MNMRQRPQPAGRGGFPN PPPVEPYNIIPIHDLLTDHPSLQSPEVRAAAAALRTVGELRR
Sbjct: 1    MNMRQRPQPAGRGGFPNQPPPVEPYNIIPIHDLLTDHPSLQSPEVRAAAAALRTVGELRR 60

Query: 61   PSFVPWNPKYDLLDWLGLFFGFQSDNVRNQREHLVLHLANSQMRLRSSPEQADLLDRTVL 120
            PSFVPWNPKYDLLDWLGLFFGFQSDNVRNQREHLVLHLANSQMRLRSSP QADLLDRTVL
Sbjct: 61   PSFVPWNPKYDLLDWLGLFFGFQSDNVRNQREHLVLHLANSQMRLRSSPVQADLLDRTVL 120

Query: 121  RNFRKKLLRSYTLWCSYLGRKSNVRFPRRDQSEERRELLYVSLYLLIWGEAANLRFVPEC 180
            RNFRKKLLRSYTLWCSY+GRKSNVRFP RDQSEERRELLYVSLYLLIWGEAANLRFVPEC
Sbjct: 121  RNFRKKLLRSYTLWCSYVGRKSNVRFPSRDQSEERRELLYVSLYLLIWGEAANLRFVPEC 180

Query: 181  LCYIYHFMAMELNQILDDYIDPETGRPYSPAIHGDCAFLKSVVMPIYQTIKTEVESSRNG 240
            LCYIYHFMAMELNQILDDYIDPETGRPYSPAIHGDCAFLKSVVMPIYQT KTEVESSRNG
Sbjct: 181  LCYIYHFMAMELNQILDDYIDPETGRPYSPAIHGDCAFLKSVVMPIYQTTKTEVESSRNG 240

Query: 241  TAPHSAWRNYDDINEYFWSRRCFHNLGWPLELSSNFFATTPKNRRVGKTGFVEQRSFWNI 300
            TAPHSAWRNYDDINEYFWSRRCFHNLGWPL LSSNFFATTPKNRRVGKTGFVEQRSFWNI
Sbjct: 241  TAPHSAWRNYDDINEYFWSRRCFHNLGWPLNLSSNFFATTPKNRRVGKTGFVEQRSFWNI 300

Query: 301  FRSFDKIWILLLLFLQASIIVAWQGHQYPWIALKSRDVQVNLLTVFITWSGLRLFQAVLD 360
            FRSFDKIW+LLLLFLQASIIVAWQGHQYPWIALKSRDVQV LLTVFITWSGLRLFQAVLD
Sbjct: 301  FRSFDKIWVLLLLFLQASIIVAWQGHQYPWIALKSRDVQVELLTVFITWSGLRLFQAVLD 360

Query: 361  AGTQYSLVSKETMWLGVRMLLKGLAAIAWIIVFAVFYARIWSQKNSDGFWSDEANGKIFI 420
            AGTQYSLVS+ETMWLGVRMLLKGLAAIAWIIVFAVFYARIWSQKNSDGFWSDEAN +IFI
Sbjct: 361  AGTQYSLVSRETMWLGVRMLLKGLAAIAWIIVFAVFYARIWSQKNSDGFWSDEANWRIFI 420

Query: 421  FLRIVFAFVIPELLALLLFVLPWIRNGLEELDWKVMYLFTWWFHTRIFVGRGLREGLIDN 480
            FLR VFAFVIPELLALLLF+LPWIRNGLEELDWK+MYLFTWWFHTRIFVGRGLREGLIDN
Sbjct: 421  FLRAVFAFVIPELLALLLFILPWIRNGLEELDWKIMYLFTWWFHTRIFVGRGLREGLIDN 480

Query: 481  IKYTIFWVAVLASKFSFSYFFQIQPLVGPTKGLLNLKGPYKWHEFFGSTNIVAVVLLWTP 540
            IKYTIFWVAVLASKFSFSYFFQIQPLVGPTKGLLNLKGPYKWHEFFGSTNIVAVVLLWTP
Sbjct: 481  IKYTIFWVAVLASKFSFSYFFQIQPLVGPTKGLLNLKGPYKWHEFFGSTNIVAVVLLWTP 540

Query: 541  VVLIYLMDLQIWYSIFSSFVGAIVGLFLHLGEIRNIGQLRLRFQFFASAMQFNLMPEEQQ 600
            VVLIYLMDLQIWYSIFSSFVGAIVGLFLHLGEIRNI QLRLRFQFFASAMQFNLMPE QQ
Sbjct: 541  VVLIYLMDLQIWYSIFSSFVGAIVGLFLHLGEIRNIDQLRLRFQFFASAMQFNLMPEVQQ 600

Query: 601  LTPKLTRLKKIRDAIHRLKLRYGLGQPYKKIESSMIDTTRFALIWNEILITMREEDLISD 660
            LTPKLTRLKKIRDAIHRLKLRYGLGQPYKKIESS IDTTRFALIWNEILITMREEDLISD
Sbjct: 601  LTPKLTRLKKIRDAIHRLKLRYGLGQPYKKIESSRIDTTRFALIWNEILITMREEDLISD 660

Query: 661  RDFDLLELPPNYWNIRVIRWPCFLLCNELLLALSQAIELGNKPDEDLWSKICKNEYQRCA 720
            RDFDLLELPPN W+IRVIRWPCFLLCNELLLALSQA EL NK DEDLW KICKNEYQRCA
Sbjct: 661  RDFDLLELPPNCWSIRVIRWPCFLLCNELLLALSQATELANKSDEDLWLKICKNEYQRCA 720

Query: 721  VIEAYDSVKALLLNIVKYGSEENSIVVKVFIDIDNVIGMGKFMEAYNPNVLQEIHTKLIS 780
            VIEAYDSVKAL+LNIVKYGSEENSIVVK+FIDIDN IG+GKFMEAYNP+VLQ+IH KLIS
Sbjct: 721  VIEAYDSVKALILNIVKYGSEENSIVVKIFIDIDNAIGLGKFMEAYNPDVLQDIHPKLIS 780

Query: 781  LVELLIGTKKDLSKAVFILQALYELSIREFPRSKKSTKQLREEGLVPRNPATDEGLLFEN 840
            LVELLIGTKKDLSKAV ILQALYELS+REFPRSKKSTKQLREEGL PRNPAT+E  LFEN
Sbjct: 781  LVELLIGTKKDLSKAVDILQALYELSVREFPRSKKSTKQLREEGLAPRNPATNEEFLFEN 840

Query: 841  AVVFPGLEDKYFYRNVQRLHTILTSRDSMHNVPSNLEARRRLAFFSNSLFMNMPRAPYVE 900
            AVVFPGLEDK FYRNVQRLHTILTSRDSMH+VPSNLEARRRLAFFSNSLFMNMPRAPYVE
Sbjct: 841  AVVFPGLEDKSFYRNVQRLHTILTSRDSMHDVPSNLEARRRLAFFSNSLFMNMPRAPYVE 900

Query: 901  KMMPFSVLTPYYDEEVVYGKEMLRSENEDGVSTLFYLQRIYEDEWRNFMERMRKKGLEHE 960
            KMMPFSVLTPYYDEEVVYGKEMLR ENEDGVSTLFYLQRIYEDEWRNFMERMRK+GLEHE
Sbjct: 901  KMMPFSVLTPYYDEEVVYGKEMLRKENEDGVSTLFYLQRIYEDEWRNFMERMRKEGLEHE 960

Query: 961  DDIWTKKSRELRLWASYRGQTLSRTVRGMMYYHRALKMFSFLDTASEMDIRMGSQEIASH 1020
            DDIWTKKSR+LRLWASYRGQTLSRTVRGMMYYHRALKMFSFLDTASEMDIRMGSQEIASH
Sbjct: 961  DDIWTKKSRDLRLWASYRGQTLSRTVRGMMYYHRALKMFSFLDTASEMDIRMGSQEIASH 1020

Query: 1021 GSITRKHALDGLHSTQPPSRNLNRASTGELLYRRSEYGTALMKFTYVVTCQVYGLQKAKR 1080
            GSITR+HALDGLHSTQPPSRNLNRASTGELLYRR EYGTALMKFTYVVTCQVYGLQKAKR
Sbjct: 1021 GSITREHALDGLHSTQPPSRNLNRASTGELLYRRYEYGTALMKFTYVVTCQVYGLQKAKR 1080

Query: 1081 DPRAEEILNLMKDNESLRVAYVDEVHRGRDEVEFYSVLVKYDQELKKEVVIYRIKLPGPL 1140
            DPRAEEILNLMKDNESLRVAYVDEVHRGRDEVEFYSVLVKYDQEL+KEVVIYRIKLPGPL
Sbjct: 1081 DPRAEEILNLMKDNESLRVAYVDEVHRGRDEVEFYSVLVKYDQELQKEVVIYRIKLPGPL 1140

Query: 1141 KIGEGKPENQNHAIIFTRGDAIQTIDMNQDNYFEEALKMRNLLEEFNNSYGIRKPTILGV 1200
            KIGEGKPENQNHAIIFTRGDAIQTIDMNQDNYFEEALKMRNLLEEF+NSYGIRKPTILGV
Sbjct: 1141 KIGEGKPENQNHAIIFTRGDAIQTIDMNQDNYFEEALKMRNLLEEFSNSYGIRKPTILGV 1200

Query: 1201 RENVFTGSVSSLAWFMSAQETSFVTLAQRVLANPLKVRMHYGHPDVFDRFWFLTRGGISK 1260
            RENVFTGSVSSLAWFMSAQETSFVTLAQRVLANPLKVRMHYGHPDVFDRFWFLTRGGISK
Sbjct: 1201 RENVFTGSVSSLAWFMSAQETSFVTLAQRVLANPLKVRMHYGHPDVFDRFWFLTRGGISK 1260

Query: 1261 ASKVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGFNQISMFEAKVASGNGEQVLS 1320
            ASKVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGFNQISMFEAKVASGNGEQVLS
Sbjct: 1261 ASKVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGFNQISMFEAKVASGNGEQVLS 1320

Query: 1321 RDIYRLGHRLDFFRVLSVFYTTVGYYFNTMLVVLSVYTFLWGRLYLALSGVEDAAVASSS 1380
            RDIYRLGHRLDFFRVLSVFYTTVGYYFNTMLVVLSVYTFLWGRLY ALSGVEDAA ASSS
Sbjct: 1321 RDIYRLGHRLDFFRVLSVFYTTVGYYFNTMLVVLSVYTFLWGRLYFALSGVEDAAAASSS 1380

Query: 1381 GNNRALGAILNQQFIIQLGLFTALPMIVENSLEHGFLPAIWNFLTMQLQLASFFYTFSLG 1440
            GNNRALGAILNQQFIIQLGLFTALPMIVENSLEHGFLPAIWNFLTMQLQLASFFYTFSLG
Sbjct: 1381 GNNRALGAILNQQFIIQLGLFTALPMIVENSLEHGFLPAIWNFLTMQLQLASFFYTFSLG 1440

Query: 1441 TRTHFFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFVKAIELGVILIVYASRS 1500
            TRTHFFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFVKAIELGVILIVYASRS
Sbjct: 1441 TRTHFFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFVKAIELGVILIVYASRS 1500

Query: 1501 PLARSTFTFVILSISSWFLIVSWIMAPFIFNPSGFDWLKTVYDFDDFINWLWNAGGVFTK 1560
            PLARSTFTF+ILSISSWFLIVSWIMAPFIFNPSGFDWLKTVYDFDDFINWLWNAGGVF+K
Sbjct: 1501 PLARSTFTFIILSISSWFLIVSWIMAPFIFNPSGFDWLKTVYDFDDFINWLWNAGGVFSK 1560

Query: 1561 AEQSWEAWWLEENSHLRSTGLWGKLLEIILDLRFFFFQYAIVYHLNITGNNTSIAVYFIS 1620
            AEQSWEAWWLEENSHLRSTGLWGKLLEIILDLRFFFFQYAIVYHLNITGN TSIAVYFIS
Sbjct: 1561 AEQSWEAWWLEENSHLRSTGLWGKLLEIILDLRFFFFQYAIVYHLNITGNKTSIAVYFIS 1620

Query: 1621 WVSMIVLVGIYIVIAYARDKYAAKEHIYYRLVQLIVIVITVLVIVILMEFTPFNTGDLVT 1680
            WVSMIVLVG+YIVIAYA+DKYAAKEHIYYRLVQLIVIVITVLVIVILMEFTPFNTGDLVT
Sbjct: 1621 WVSMIVLVGVYIVIAYAQDKYAAKEHIYYRLVQLIVIVITVLVIVILMEFTPFNTGDLVT 1680

Query: 1681 CLLAFIPTGWGIISIAQVLRPFLQTTVVWDTVVSMARLYDLLFGVIAMAPLALLSWLPGF 1740
            CLLAFIPTGWGIISIAQVLRPFLQTTVVWDTV+S+ARLYDLLFG+IAMAPLALLSWLPGF
Sbjct: 1681 CLLAFIPTGWGIISIAQVLRPFLQTTVVWDTVISLARLYDLLFGMIAMAPLALLSWLPGF 1740

Query: 1741 QSMQTRILFNEAFSRGLQISRIIAGKKTV 1770
            QSMQTRILFNEAFSRGLQISRIIAGKKTV
Sbjct: 1741 QSMQTRILFNEAFSRGLQISRIIAGKKTV 1769

BLAST of HG10021216 vs. NCBI nr
Match: XP_008442573.1 (PREDICTED: callose synthase 11 [Cucumis melo])

HSP 1 Score: 3394.7 bits (8801), Expect = 0.0e+00
Identity = 1695/1769 (95.82%), Postives = 1728/1769 (97.68%), Query Frame = 0

Query: 1    MNMRQRPQPAGRGGFPNPPPPVEPYNIIPIHDLLTDHPSLQSPEVRAAAAALRTVGELRR 60
            MNMRQRPQ AGRGGFPNPPPPVEPYNIIPIHDLLTDHPSLQ PEVRAAAAALRTVGELRR
Sbjct: 1    MNMRQRPQAAGRGGFPNPPPPVEPYNIIPIHDLLTDHPSLQLPEVRAAAAALRTVGELRR 60

Query: 61   PSFVPWNPKYDLLDWLGLFFGFQSDNVRNQREHLVLHLANSQMRLRSSPEQADLLDRTVL 120
            PSFVPWNPKYDLLDWLGLFFGFQ+DNVRNQREHLVLHLANSQMRLRSSPEQ D+LDRTVL
Sbjct: 61   PSFVPWNPKYDLLDWLGLFFGFQNDNVRNQREHLVLHLANSQMRLRSSPEQPDVLDRTVL 120

Query: 121  RNFRKKLLRSYTLWCSYLGRKSNVRFPRRDQSEERRELLYVSLYLLIWGEAANLRFVPEC 180
            RNFRKKLLRSY+LWCSYLGRKSNVRFP RDQSEERRELLYVSLYLLIWGEAANLRF+PEC
Sbjct: 121  RNFRKKLLRSYSLWCSYLGRKSNVRFPSRDQSEERRELLYVSLYLLIWGEAANLRFLPEC 180

Query: 181  LCYIYHFMAMELNQILDDYIDPETGRPYSPAIHGDCAFLKSVVMPIYQTIKTEVESSRNG 240
            L YIYHFMAMELNQILDDYIDP+TGRPYSPAIHGDCAFLKSVVMPIYQTIK EVESSRNG
Sbjct: 181  LSYIYHFMAMELNQILDDYIDPDTGRPYSPAIHGDCAFLKSVVMPIYQTIKIEVESSRNG 240

Query: 241  TAPHSAWRNYDDINEYFWSRRCFHNLGWPLELSSNFFATTPKNRRVGKTGFVEQRSFWNI 300
            +APHSAWRNYDDINEYFWSRRCF +LGWPL LSSNFFATT KNRRVGKTGFVEQRSFWNI
Sbjct: 241  SAPHSAWRNYDDINEYFWSRRCFRSLGWPLNLSSNFFATTDKNRRVGKTGFVEQRSFWNI 300

Query: 301  FRSFDKIWILLLLFLQASIIVAWQGHQYPWIALKSRDVQVNLLTVFITWSGLRLFQAVLD 360
            FRSFDKIW+LLLLFLQASIIVAWQGHQYPWIALKSRDVQV LLTVFITWSG+R FQAVLD
Sbjct: 301  FRSFDKIWVLLLLFLQASIIVAWQGHQYPWIALKSRDVQVELLTVFITWSGMRFFQAVLD 360

Query: 361  AGTQYSLVSKETMWLGVRMLLKGLAAIAWIIVFAVFYARIWSQKNSDGFWSDEANGKIFI 420
            AGTQYSLVS+ETMWLGVRMLLKGLAA+AWIIVF+VFYARIW+QKNSDGFWSDEAN KIFI
Sbjct: 361  AGTQYSLVSRETMWLGVRMLLKGLAAVAWIIVFSVFYARIWNQKNSDGFWSDEANAKIFI 420

Query: 421  FLRIVFAFVIPELLALLLFVLPWIRNGLEELDWKVMYLFTWWFHTRIFVGRGLREGLIDN 480
            FLR VFAFVIPELLAL+ FVLPWIRNGLE LDWKVMYLFTWWFHTRIFVGRGLREGLIDN
Sbjct: 421  FLRAVFAFVIPELLALIFFVLPWIRNGLEGLDWKVMYLFTWWFHTRIFVGRGLREGLIDN 480

Query: 481  IKYTIFWVAVLASKFSFSYFFQIQPLVGPTKGLLNLKGPYKWHEFFGSTNIVAVVLLWTP 540
            IKYTIFWVAVLASKFSFSYFFQIQPLVGPTKGLLNLKGPYKWHEFFGSTNIVAVVLLWTP
Sbjct: 481  IKYTIFWVAVLASKFSFSYFFQIQPLVGPTKGLLNLKGPYKWHEFFGSTNIVAVVLLWTP 540

Query: 541  VVLIYLMDLQIWYSIFSSFVGAIVGLFLHLGEIRNIGQLRLRFQFFASAMQFNLMPEEQQ 600
            VVLIYLMDLQIWYSIFSSFVGAIVGLFLHLGEIRNIGQLRLRFQFFASAMQFNLMPE QQ
Sbjct: 541  VVLIYLMDLQIWYSIFSSFVGAIVGLFLHLGEIRNIGQLRLRFQFFASAMQFNLMPEVQQ 600

Query: 601  LTPKLTRLKKIRDAIHRLKLRYGLGQPYKKIESSMIDTTRFALIWNEILITMREEDLISD 660
            LTPKLTRLKKIRDAIHRLKLRYGLG  YKKIESS IDTT+FALIWNEILITMREEDLISD
Sbjct: 601  LTPKLTRLKKIRDAIHRLKLRYGLGLSYKKIESSRIDTTKFALIWNEILITMREEDLISD 660

Query: 661  RDFDLLELPPNYWNIRVIRWPCFLLCNELLLALSQAIELGNKPDEDLWSKICKNEYQRCA 720
            RDFDLLELPPNYW+IRVIRWPC LLCNELLLALSQA EL NKPDEDLW KICKNEYQRCA
Sbjct: 661  RDFDLLELPPNYWSIRVIRWPCVLLCNELLLALSQATELANKPDEDLWLKICKNEYQRCA 720

Query: 721  VIEAYDSVKALLLNIVKYGSEENSIVVKVFIDIDNVIGMGKFMEAYNPNVLQEIHTKLIS 780
            VIEAYDSVKALLL+IVKYGSEENSIVVK+FID+DN IG+GKFMEAYNPNVL EIH KLIS
Sbjct: 721  VIEAYDSVKALLLSIVKYGSEENSIVVKIFIDLDNAIGLGKFMEAYNPNVLPEIHAKLIS 780

Query: 781  LVELLIGTKKDLSKAVFILQALYELSIREFPRSKKSTKQLREEGLVPRNPATDEGLLFEN 840
            LVELLIGTKKDLSKAV ILQALYELSIREFPRSKKSTKQLREEGLVPRNPAT E  LFEN
Sbjct: 781  LVELLIGTKKDLSKAVDILQALYELSIREFPRSKKSTKQLREEGLVPRNPATYEEFLFEN 840

Query: 841  AVVFPGLEDKYFYRNVQRLHTILTSRDSMHNVPSNLEARRRLAFFSNSLFMNMPRAPYVE 900
            AVVFP +EDK+F RNVQRLHTILTSRDSMHNVPSNLEARRR+AFFSNSLFMNMPRAPYVE
Sbjct: 841  AVVFPSVEDKFFNRNVQRLHTILTSRDSMHNVPSNLEARRRIAFFSNSLFMNMPRAPYVE 900

Query: 901  KMMPFSVLTPYYDEEVVYGKEMLRSENEDGVSTLFYLQRIYEDEWRNFMERMRKKGLEHE 960
            KMMPFSVLTPYYDEEVVYGKEMLRSENEDGVSTLFYLQRIYEDEWRNFMERMRK+GLEHE
Sbjct: 901  KMMPFSVLTPYYDEEVVYGKEMLRSENEDGVSTLFYLQRIYEDEWRNFMERMRKEGLEHE 960

Query: 961  DDIWTKKSRELRLWASYRGQTLSRTVRGMMYYHRALKMFSFLDTASEMDIRMGSQEIASH 1020
            DDIWTKKSR+LRLWASYRGQTLSRTVRGMMYYHRALKMFSFLDTASEMDIRMGSQEIASH
Sbjct: 961  DDIWTKKSRDLRLWASYRGQTLSRTVRGMMYYHRALKMFSFLDTASEMDIRMGSQEIASH 1020

Query: 1021 GSITRKHALDGLHSTQPPSRNLNRASTGELLYRRSEYGTALMKFTYVVTCQVYGLQKAKR 1080
            GSITRKHA DGLHSTQP SR+LNRASTGE L+RRSEYGTALMKFTYVVTCQVYGLQKAKR
Sbjct: 1021 GSITRKHASDGLHSTQPASRDLNRASTGEWLHRRSEYGTALMKFTYVVTCQVYGLQKAKR 1080

Query: 1081 DPRAEEILNLMKDNESLRVAYVDEVHRGRDEVEFYSVLVKYDQELKKEVVIYRIKLPGPL 1140
            DPRAEEILNLMKDNESLRVAYVDEVH GRDEVEFYSVLVKYDQEL+KEVVIYRIKLPGPL
Sbjct: 1081 DPRAEEILNLMKDNESLRVAYVDEVHHGRDEVEFYSVLVKYDQELQKEVVIYRIKLPGPL 1140

Query: 1141 KIGEGKPENQNHAIIFTRGDAIQTIDMNQDNYFEEALKMRNLLEEFNNSYGIRKPTILGV 1200
            KIGEGKPENQNHAIIFTRGDAIQTIDMNQDNYFEEALKMRNLLEEFN SYGIRKPTILGV
Sbjct: 1141 KIGEGKPENQNHAIIFTRGDAIQTIDMNQDNYFEEALKMRNLLEEFNKSYGIRKPTILGV 1200

Query: 1201 RENVFTGSVSSLAWFMSAQETSFVTLAQRVLANPLKVRMHYGHPDVFDRFWFLTRGGISK 1260
            RENVFTGSVSSLAWFMSAQETSFVTLAQRVLANPLKVRMHYGHPDVFDRFWFLTRGGISK
Sbjct: 1201 RENVFTGSVSSLAWFMSAQETSFVTLAQRVLANPLKVRMHYGHPDVFDRFWFLTRGGISK 1260

Query: 1261 ASKVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGFNQISMFEAKVASGNGEQVLS 1320
            ASKVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGFNQISMFEAKVASGNGEQVLS
Sbjct: 1261 ASKVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGFNQISMFEAKVASGNGEQVLS 1320

Query: 1321 RDIYRLGHRLDFFRVLSVFYTTVGYYFNTMLVVLSVYTFLWGRLYLALSGVEDAAVASSS 1380
            RDIYRLGHRLDFFRVLSVFYTTVGYYFNTMLVVLSVYTFLWGRLYLALSGVEDAA+ASS+
Sbjct: 1321 RDIYRLGHRLDFFRVLSVFYTTVGYYFNTMLVVLSVYTFLWGRLYLALSGVEDAAIASST 1380

Query: 1381 GNNRALGAILNQQFIIQLGLFTALPMIVENSLEHGFLPAIWNFLTMQLQLASFFYTFSLG 1440
            GNNRALGAILNQQFIIQLGLFTALPMIVENSLEHGFLPAIWNFLTMQLQLASFFYTFSLG
Sbjct: 1381 GNNRALGAILNQQFIIQLGLFTALPMIVENSLEHGFLPAIWNFLTMQLQLASFFYTFSLG 1440

Query: 1441 TRTHFFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFVKAIELGVILIVYASRS 1500
            TRTHFFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFVKAIELGVILIVYASRS
Sbjct: 1441 TRTHFFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFVKAIELGVILIVYASRS 1500

Query: 1501 PLARSTFTFVILSISSWFLIVSWIMAPFIFNPSGFDWLKTVYDFDDFINWLWNAGGVFTK 1560
            PLA STFTFVILSISSWFLIVSWIMAPFIFNPSGFDWLKTVYDFDDFINWLWNAGGVFTK
Sbjct: 1501 PLATSTFTFVILSISSWFLIVSWIMAPFIFNPSGFDWLKTVYDFDDFINWLWNAGGVFTK 1560

Query: 1561 AEQSWEAWWLEENSHLRSTGLWGKLLEIILDLRFFFFQYAIVYHLNITGNNTSIAVYFIS 1620
            AEQSWEAWWLEENSHLRSTGLWGKLLEIILDLRFFFFQYAIVYHLNITGNNTSIAVYFIS
Sbjct: 1561 AEQSWEAWWLEENSHLRSTGLWGKLLEIILDLRFFFFQYAIVYHLNITGNNTSIAVYFIS 1620

Query: 1621 WVSMIVLVGIYIVIAYARDKYAAKEHIYYRLVQLIVIVITVLVIVILMEFTPFNTGDLVT 1680
            WVSMI LVGIYIVIAYA+DKYAAKEHIYYRLVQLIVIVITVLVIVILMEFTPFN GDLVT
Sbjct: 1621 WVSMIALVGIYIVIAYAQDKYAAKEHIYYRLVQLIVIVITVLVIVILMEFTPFNVGDLVT 1680

Query: 1681 CLLAFIPTGWGIISIAQVLRPFLQTTVVWDTVVSMARLYDLLFGVIAMAPLALLSWLPGF 1740
            CLLAFIPTGWGIISIAQVLRPFLQTTVVWDTVVS+ARLYDLLFG+I MAPLALLSWLPGF
Sbjct: 1681 CLLAFIPTGWGIISIAQVLRPFLQTTVVWDTVVSLARLYDLLFGMIVMAPLALLSWLPGF 1740

Query: 1741 QSMQTRILFNEAFSRGLQISRIIAGKKTV 1770
            QSMQTRILFNEAFSRGLQISRIIAGKKTV
Sbjct: 1741 QSMQTRILFNEAFSRGLQISRIIAGKKTV 1769

BLAST of HG10021216 vs. NCBI nr
Match: KAA0056989.1 (callose synthase 11 [Cucumis melo var. makuwa] >TYK26417.1 callose synthase 11 [Cucumis melo var. makuwa])

HSP 1 Score: 3384.3 bits (8774), Expect = 0.0e+00
Identity = 1690/1764 (95.80%), Postives = 1723/1764 (97.68%), Query Frame = 0

Query: 6    RPQPAGRGGFPNPPPPVEPYNIIPIHDLLTDHPSLQSPEVRAAAAALRTVGELRRPSFVP 65
            RPQ AGRGGFPNPPPPVEPYNIIPIHDLLTDHPSLQ PEVRAAAAALRTVGELRRPSFVP
Sbjct: 65   RPQAAGRGGFPNPPPPVEPYNIIPIHDLLTDHPSLQLPEVRAAAAALRTVGELRRPSFVP 124

Query: 66   WNPKYDLLDWLGLFFGFQSDNVRNQREHLVLHLANSQMRLRSSPEQADLLDRTVLRNFRK 125
            WNPKYDLLDWLGLFFGFQ+DNVRNQREHLVLHLANSQMRLRSSPEQ D+LDRTVLRNFRK
Sbjct: 125  WNPKYDLLDWLGLFFGFQNDNVRNQREHLVLHLANSQMRLRSSPEQPDVLDRTVLRNFRK 184

Query: 126  KLLRSYTLWCSYLGRKSNVRFPRRDQSEERRELLYVSLYLLIWGEAANLRFVPECLCYIY 185
            KLLRSY+LWCSYLGRKSNVRFP RDQSEERRELLYVSLYLLIWGEAANLRF+PECL YIY
Sbjct: 185  KLLRSYSLWCSYLGRKSNVRFPSRDQSEERRELLYVSLYLLIWGEAANLRFLPECLSYIY 244

Query: 186  HFMAMELNQILDDYIDPETGRPYSPAIHGDCAFLKSVVMPIYQTIKTEVESSRNGTAPHS 245
            HFMAMELNQILDDYIDP+TGRPYSPAIHGDCAFLKSVVMPIYQTIK EVESSRNG+APHS
Sbjct: 245  HFMAMELNQILDDYIDPDTGRPYSPAIHGDCAFLKSVVMPIYQTIKIEVESSRNGSAPHS 304

Query: 246  AWRNYDDINEYFWSRRCFHNLGWPLELSSNFFATTPKNRRVGKTGFVEQRSFWNIFRSFD 305
            AWRNYDDINEYFWSRRCF +LGWPL LSSNFFATT KNRRVGKTGFVEQRSFWNIFRSFD
Sbjct: 305  AWRNYDDINEYFWSRRCFRSLGWPLNLSSNFFATTDKNRRVGKTGFVEQRSFWNIFRSFD 364

Query: 306  KIWILLLLFLQASIIVAWQGHQYPWIALKSRDVQVNLLTVFITWSGLRLFQAVLDAGTQY 365
            KIW+LLLLFLQASIIVAWQGHQYPWIALKSRDVQV LLTVFITWSG+R FQAVLDAGTQY
Sbjct: 365  KIWVLLLLFLQASIIVAWQGHQYPWIALKSRDVQVELLTVFITWSGMRFFQAVLDAGTQY 424

Query: 366  SLVSKETMWLGVRMLLKGLAAIAWIIVFAVFYARIWSQKNSDGFWSDEANGKIFIFLRIV 425
            SLVS+ETMWLGVRMLLKGLAA+AWIIVF+VFYARIW+QKNSDGFWSDEAN KIFIFLR V
Sbjct: 425  SLVSRETMWLGVRMLLKGLAAVAWIIVFSVFYARIWNQKNSDGFWSDEANAKIFIFLRAV 484

Query: 426  FAFVIPELLALLLFVLPWIRNGLEELDWKVMYLFTWWFHTRIFVGRGLREGLIDNIKYTI 485
            FAFVIPELLAL+ FVLPWIRNGLE LDWKVMYLFTWWFHTRIFVGRGLREGLIDNIKYTI
Sbjct: 485  FAFVIPELLALIFFVLPWIRNGLEGLDWKVMYLFTWWFHTRIFVGRGLREGLIDNIKYTI 544

Query: 486  FWVAVLASKFSFSYFFQIQPLVGPTKGLLNLKGPYKWHEFFGSTNIVAVVLLWTPVVLIY 545
            FWVAVLASKFSFSYFFQIQPLVGPTKGLLNLKGPYKWHEFFGSTNIVAVVLLWTPVVLIY
Sbjct: 545  FWVAVLASKFSFSYFFQIQPLVGPTKGLLNLKGPYKWHEFFGSTNIVAVVLLWTPVVLIY 604

Query: 546  LMDLQIWYSIFSSFVGAIVGLFLHLGEIRNIGQLRLRFQFFASAMQFNLMPEEQQLTPKL 605
            LMDLQIWYSIFSSFVGAIVGLFLHLGEIRNIGQLRLRFQFFASAMQFNLMPE QQLTPKL
Sbjct: 605  LMDLQIWYSIFSSFVGAIVGLFLHLGEIRNIGQLRLRFQFFASAMQFNLMPEVQQLTPKL 664

Query: 606  TRLKKIRDAIHRLKLRYGLGQPYKKIESSMIDTTRFALIWNEILITMREEDLISDRDFDL 665
            TRLKKIRDAIHRLKLRYGLG  YKKIESS IDTT+FALIWNEILITMREEDLISDRDFDL
Sbjct: 665  TRLKKIRDAIHRLKLRYGLGLSYKKIESSRIDTTKFALIWNEILITMREEDLISDRDFDL 724

Query: 666  LELPPNYWNIRVIRWPCFLLCNELLLALSQAIELGNKPDEDLWSKICKNEYQRCAVIEAY 725
            LELPPNYW+IRVIRWPC LLCNELLLALSQA EL NKPDEDLW KICKNEYQRCAVIEAY
Sbjct: 725  LELPPNYWSIRVIRWPCVLLCNELLLALSQATELANKPDEDLWLKICKNEYQRCAVIEAY 784

Query: 726  DSVKALLLNIVKYGSEENSIVVKVFIDIDNVIGMGKFMEAYNPNVLQEIHTKLISLVELL 785
            DSVKALLL+IVKYGSEENSIVVK+FID+DN IG+GKFMEAYNPNVL EIH KLISLVELL
Sbjct: 785  DSVKALLLSIVKYGSEENSIVVKIFIDLDNAIGLGKFMEAYNPNVLPEIHAKLISLVELL 844

Query: 786  IGTKKDLSKAVFILQALYELSIREFPRSKKSTKQLREEGLVPRNPATDEGLLFENAVVFP 845
            IGTKKDLSKAV ILQALYELSIREFPRSKKSTKQLREEGLVPRNPAT E  LFENAVVFP
Sbjct: 845  IGTKKDLSKAVDILQALYELSIREFPRSKKSTKQLREEGLVPRNPATYEEFLFENAVVFP 904

Query: 846  GLEDKYFYRNVQRLHTILTSRDSMHNVPSNLEARRRLAFFSNSLFMNMPRAPYVEKMMPF 905
             +EDK+F RNVQRLHTILTSRDSMHNVPSNLEARRR+AFFSNSLFMNMPRAPYVEKMMPF
Sbjct: 905  SVEDKFFNRNVQRLHTILTSRDSMHNVPSNLEARRRIAFFSNSLFMNMPRAPYVEKMMPF 964

Query: 906  SVLTPYYDEEVVYGKEMLRSENEDGVSTLFYLQRIYEDEWRNFMERMRKKGLEHEDDIWT 965
            SVLTPYYDEEVVYGKEMLRSENEDGVSTLFYLQRIYEDEWRNFMERMRK+GLEHEDDIWT
Sbjct: 965  SVLTPYYDEEVVYGKEMLRSENEDGVSTLFYLQRIYEDEWRNFMERMRKEGLEHEDDIWT 1024

Query: 966  KKSRELRLWASYRGQTLSRTVRGMMYYHRALKMFSFLDTASEMDIRMGSQEIASHGSITR 1025
            KKSR+LRLWASYRGQTLSRTVRGMMYYHRALKMFSFLDTASEMDIRMGSQEIASHGSITR
Sbjct: 1025 KKSRDLRLWASYRGQTLSRTVRGMMYYHRALKMFSFLDTASEMDIRMGSQEIASHGSITR 1084

Query: 1026 KHALDGLHSTQPPSRNLNRASTGELLYRRSEYGTALMKFTYVVTCQVYGLQKAKRDPRAE 1085
            KHA DGLHSTQP SR+LNRASTGE L+RRSEYGTALMKFTYVVTCQVYGLQKAKRDPRAE
Sbjct: 1085 KHASDGLHSTQPASRDLNRASTGEWLHRRSEYGTALMKFTYVVTCQVYGLQKAKRDPRAE 1144

Query: 1086 EILNLMKDNESLRVAYVDEVHRGRDEVEFYSVLVKYDQELKKEVVIYRIKLPGPLKIGEG 1145
            EILNLMKDNESLRVAYVDEVH GRDEVEFYSVLVKYDQEL+KEVVIYRIKLPGPLKIGEG
Sbjct: 1145 EILNLMKDNESLRVAYVDEVHHGRDEVEFYSVLVKYDQELQKEVVIYRIKLPGPLKIGEG 1204

Query: 1146 KPENQNHAIIFTRGDAIQTIDMNQDNYFEEALKMRNLLEEFNNSYGIRKPTILGVRENVF 1205
            KPENQNHAIIFTRGDAIQTIDMNQDNYFEEALKMRNLLEEFN SYGIRKPTILGVRENVF
Sbjct: 1205 KPENQNHAIIFTRGDAIQTIDMNQDNYFEEALKMRNLLEEFNKSYGIRKPTILGVRENVF 1264

Query: 1206 TGSVSSLAWFMSAQETSFVTLAQRVLANPLKVRMHYGHPDVFDRFWFLTRGGISKASKVI 1265
            TGSVSSLAWFMSAQETSFVTLAQRVLANPLKVRMHYGHPDVFDRFWFLTRGGISKASKVI
Sbjct: 1265 TGSVSSLAWFMSAQETSFVTLAQRVLANPLKVRMHYGHPDVFDRFWFLTRGGISKASKVI 1324

Query: 1266 NISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGFNQISMFEAKVASGNGEQVLSRDIYR 1325
            NISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGFNQISMFEAKVASGNGEQVLSRDIYR
Sbjct: 1325 NISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGFNQISMFEAKVASGNGEQVLSRDIYR 1384

Query: 1326 LGHRLDFFRVLSVFYTTVGYYFNTMLVVLSVYTFLWGRLYLALSGVEDAAVASSSGNNRA 1385
            LGHRLDFFRVLSVFYTTVGYYFNTMLVVLSVYTFLWGRLYLALSGVEDAA+ASS+GNNRA
Sbjct: 1385 LGHRLDFFRVLSVFYTTVGYYFNTMLVVLSVYTFLWGRLYLALSGVEDAAIASSTGNNRA 1444

Query: 1386 LGAILNQQFIIQLGLFTALPMIVENSLEHGFLPAIWNFLTMQLQLASFFYTFSLGTRTHF 1445
            LGAILNQQFIIQLGLFTALPMIVENSLEHGFLPAIWNFLTMQLQLASFFYTFSLGTRTHF
Sbjct: 1445 LGAILNQQFIIQLGLFTALPMIVENSLEHGFLPAIWNFLTMQLQLASFFYTFSLGTRTHF 1504

Query: 1446 FGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFVKAIELGVILIVYASRSPLARS 1505
            FGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFVKAIELGVILIVYASRSPLA S
Sbjct: 1505 FGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFVKAIELGVILIVYASRSPLATS 1564

Query: 1506 TFTFVILSISSWFLIVSWIMAPFIFNPSGFDWLKTVYDFDDFINWLWNAGGVFTKAEQSW 1565
            TFTFVILSISSWFLIVSWIMAPFIFNPSGFDWLKTVYDFDDFINWLWNAGGVFTKAEQSW
Sbjct: 1565 TFTFVILSISSWFLIVSWIMAPFIFNPSGFDWLKTVYDFDDFINWLWNAGGVFTKAEQSW 1624

Query: 1566 EAWWLEENSHLRSTGLWGKLLEIILDLRFFFFQYAIVYHLNITGNNTSIAVYFISWVSMI 1625
            EAWWLEENSHLRSTGLWGKLLEIILDLRFFFFQYAIVYHLNITGNNTSIAVYFISWVSMI
Sbjct: 1625 EAWWLEENSHLRSTGLWGKLLEIILDLRFFFFQYAIVYHLNITGNNTSIAVYFISWVSMI 1684

Query: 1626 VLVGIYIVIAYARDKYAAKEHIYYRLVQLIVIVITVLVIVILMEFTPFNTGDLVTCLLAF 1685
             LVGIYIVIAYA+DKYAAKEHIYYRLVQLIVIVITVLVIVILMEFTPFN GDLVTCLLAF
Sbjct: 1685 ALVGIYIVIAYAQDKYAAKEHIYYRLVQLIVIVITVLVIVILMEFTPFNVGDLVTCLLAF 1744

Query: 1686 IPTGWGIISIAQVLRPFLQTTVVWDTVVSMARLYDLLFGVIAMAPLALLSWLPGFQSMQT 1745
            IPTGWGIISIAQVLRPFLQTTVVWDTVVS+ARLYDLLFG+I MAPLALLSWLPGFQSMQT
Sbjct: 1745 IPTGWGIISIAQVLRPFLQTTVVWDTVVSLARLYDLLFGMIVMAPLALLSWLPGFQSMQT 1804

Query: 1746 RILFNEAFSRGLQISRIIAGKKTV 1770
            RILFNEAFSRGLQISRIIAGKKTV
Sbjct: 1805 RILFNEAFSRGLQISRIIAGKKTV 1828

BLAST of HG10021216 vs. NCBI nr
Match: XP_004146651.1 (callose synthase 11 isoform X1 [Cucumis sativus] >XP_031741882.1 callose synthase 11 isoform X1 [Cucumis sativus])

HSP 1 Score: 3354.7 bits (8697), Expect = 0.0e+00
Identity = 1668/1769 (94.29%), Postives = 1718/1769 (97.12%), Query Frame = 0

Query: 1    MNMRQRPQPAGRGGFPNPPPPVEPYNIIPIHDLLTDHPSLQSPEVRAAAAALRTVGELRR 60
            M MRQRPQ AGRGGFPNP PPVEPYNIIPIHDLLTDHPSLQS EVRAAAAALRTVGELRR
Sbjct: 1    MTMRQRPQTAGRGGFPNPLPPVEPYNIIPIHDLLTDHPSLQSTEVRAAAAALRTVGELRR 60

Query: 61   PSFVPWNPKYDLLDWLGLFFGFQSDNVRNQREHLVLHLANSQMRLRSSPEQADLLDRTVL 120
            PSFVPWNPKYDLLDWLGLFFGFQ+DNVRNQREHLVLHLANSQMRLRSSPE  D+LDRTVL
Sbjct: 61   PSFVPWNPKYDLLDWLGLFFGFQNDNVRNQREHLVLHLANSQMRLRSSPEHPDVLDRTVL 120

Query: 121  RNFRKKLLRSYTLWCSYLGRKSNVRFPRRDQSEERRELLYVSLYLLIWGEAANLRFVPEC 180
            RNFRKKLLRSY+LWCSYLGRKSNVRFP RDQSEERRELLYVSLYLLIWGEAANLRF+PEC
Sbjct: 121  RNFRKKLLRSYSLWCSYLGRKSNVRFPSRDQSEERRELLYVSLYLLIWGEAANLRFLPEC 180

Query: 181  LCYIYHFMAMELNQILDDYIDPETGRPYSPAIHGDCAFLKSVVMPIYQTIKTEVESSRNG 240
            L YIYHFMAMELNQILDDYIDP+TGRPYSPAIHGDCAFLKSVVMPIYQTIK EVESSRNG
Sbjct: 181  LSYIYHFMAMELNQILDDYIDPDTGRPYSPAIHGDCAFLKSVVMPIYQTIKIEVESSRNG 240

Query: 241  TAPHSAWRNYDDINEYFWSRRCFHNLGWPLELSSNFFATTPKNRRVGKTGFVEQRSFWNI 300
            +APHSAWRNYDDINEYFWSRRCF +LGWPL LSSNFFATT KNRRVGKTGFVEQRSFWNI
Sbjct: 241  SAPHSAWRNYDDINEYFWSRRCFRSLGWPLNLSSNFFATTDKNRRVGKTGFVEQRSFWNI 300

Query: 301  FRSFDKIWILLLLFLQASIIVAWQGHQYPWIALKSRDVQVNLLTVFITWSGLRLFQAVLD 360
            FRSFDKIW+LLLLFLQASIIVAWQGHQYPWI LKSRDVQV LLTVFITWSG+RLFQAVLD
Sbjct: 301  FRSFDKIWVLLLLFLQASIIVAWQGHQYPWITLKSRDVQVELLTVFITWSGMRLFQAVLD 360

Query: 361  AGTQYSLVSKETMWLGVRMLLKGLAAIAWIIVFAVFYARIWSQKNSDGFWSDEANGKIFI 420
            AGTQYSLVS+ET+WLGVRMLLK LAA+AWIIVF+VFYARIWSQKNSDGFWSDEA   IF 
Sbjct: 361  AGTQYSLVSRETVWLGVRMLLKCLAAVAWIIVFSVFYARIWSQKNSDGFWSDEATANIFT 420

Query: 421  FLRIVFAFVIPELLALLLFVLPWIRNGLEELDWKVMYLFTWWFHTRIFVGRGLREGLIDN 480
            FLR VFAFVIPELLALL FVLPWIRNGLEELDWKV+YLFTWWFHTRIFVGRGLREGL+DN
Sbjct: 421  FLRAVFAFVIPELLALLFFVLPWIRNGLEELDWKVLYLFTWWFHTRIFVGRGLREGLVDN 480

Query: 481  IKYTIFWVAVLASKFSFSYFFQIQPLVGPTKGLLNLKGPYKWHEFFGSTNIVAVVLLWTP 540
            IKYTIFW+AVLASKFSFSYFFQIQPLVGPTKGLLNLKGPYKWHEFFGSTNIVAVVLLWTP
Sbjct: 481  IKYTIFWIAVLASKFSFSYFFQIQPLVGPTKGLLNLKGPYKWHEFFGSTNIVAVVLLWTP 540

Query: 541  VVLIYLMDLQIWYSIFSSFVGAIVGLFLHLGEIRNIGQLRLRFQFFASAMQFNLMPEEQQ 600
            VVL+YLMDLQIWYSIFSSFVGAIVGLFLHLGEIRNI QLRLRFQFFASAMQFNLMPE Q+
Sbjct: 541  VVLVYLMDLQIWYSIFSSFVGAIVGLFLHLGEIRNIDQLRLRFQFFASAMQFNLMPEVQE 600

Query: 601  LTPKLTRLKKIRDAIHRLKLRYGLGQPYKKIESSMIDTTRFALIWNEILITMREEDLISD 660
            LTPKLTRLKKIRDAIHRLKLRYGLG  YKKIESS IDTT+FALIWNEILITMREEDLISD
Sbjct: 601  LTPKLTRLKKIRDAIHRLKLRYGLGLSYKKIESSRIDTTKFALIWNEILITMREEDLISD 660

Query: 661  RDFDLLELPPNYWNIRVIRWPCFLLCNELLLALSQAIELGNKPDEDLWSKICKNEYQRCA 720
            RDFDLLELPPNYW+IRVIRWPC LLCNELLLALSQA EL + PDE+LW KICKNEYQRCA
Sbjct: 661  RDFDLLELPPNYWSIRVIRWPCVLLCNELLLALSQATELADNPDENLWLKICKNEYQRCA 720

Query: 721  VIEAYDSVKALLLNIVKYGSEENSIVVKVFIDIDNVIGMGKFMEAYNPNVLQEIHTKLIS 780
            VIEAYDSVKALLLNIVKYGSEENSIVVK+FID+DN IG+GKFMEAYNPNVL EIH KLIS
Sbjct: 721  VIEAYDSVKALLLNIVKYGSEENSIVVKIFIDLDNAIGLGKFMEAYNPNVLPEIHAKLIS 780

Query: 781  LVELLIGTKKDLSKAVFILQALYELSIREFPRSKKSTKQLREEGLVPRNPATDEGLLFEN 840
            LVELLIGTKKD+++AVFILQALYELSIREFPRSKKSTKQLREEGLVPRNPATDE  +FEN
Sbjct: 781  LVELLIGTKKDMTQAVFILQALYELSIREFPRSKKSTKQLREEGLVPRNPATDEEFIFEN 840

Query: 841  AVVFPGLEDKYFYRNVQRLHTILTSRDSMHNVPSNLEARRRLAFFSNSLFMNMPRAPYVE 900
            AVVFP +ED++FYRNVQRLHTILTSRDSMHNVPSNLEARRR+AFFSNSLFMNMPRAPYVE
Sbjct: 841  AVVFPSVEDRFFYRNVQRLHTILTSRDSMHNVPSNLEARRRIAFFSNSLFMNMPRAPYVE 900

Query: 901  KMMPFSVLTPYYDEEVVYGKEMLRSENEDGVSTLFYLQRIYEDEWRNFMERMRKKGLEHE 960
            KMMPFSVLTPYYDEEVVYGKEMLRSENEDGVSTLFYLQRIYEDEWRNFMERMRK+GLEHE
Sbjct: 901  KMMPFSVLTPYYDEEVVYGKEMLRSENEDGVSTLFYLQRIYEDEWRNFMERMRKEGLEHE 960

Query: 961  DDIWTKKSRELRLWASYRGQTLSRTVRGMMYYHRALKMFSFLDTASEMDIRMGSQEIASH 1020
            DDIWTKKSR++RLWASYRGQTLSRTVRGMMYYHRAL MFSFLD ASE+DIR GSQEIASH
Sbjct: 961  DDIWTKKSRDVRLWASYRGQTLSRTVRGMMYYHRALNMFSFLDKASEIDIRKGSQEIASH 1020

Query: 1021 GSITRKHALDGLHSTQPPSRNLNRASTGELLYRRSEYGTALMKFTYVVTCQVYGLQKAKR 1080
            GSITRKHALDGL STQPPS +LNRAS GE L+RRS+YG ALMKFTYVVTCQVYGLQKAKR
Sbjct: 1021 GSITRKHALDGLRSTQPPSMDLNRASIGEWLHRRSDYGIALMKFTYVVTCQVYGLQKAKR 1080

Query: 1081 DPRAEEILNLMKDNESLRVAYVDEVHRGRDEVEFYSVLVKYDQELKKEVVIYRIKLPGPL 1140
            DPRAEEILNLMKDNESLRVAYVDEVHRGRDEVEFYSVLVKYDQE  KEVVIYRIKLPGPL
Sbjct: 1081 DPRAEEILNLMKDNESLRVAYVDEVHRGRDEVEFYSVLVKYDQEQGKEVVIYRIKLPGPL 1140

Query: 1141 KIGEGKPENQNHAIIFTRGDAIQTIDMNQDNYFEEALKMRNLLEEFNNSYGIRKPTILGV 1200
            KIGEGKPENQNHAIIFTRGDA+QTIDMNQDNYFEEALKMRNLLEEFN SYGIRKPTILGV
Sbjct: 1141 KIGEGKPENQNHAIIFTRGDALQTIDMNQDNYFEEALKMRNLLEEFNKSYGIRKPTILGV 1200

Query: 1201 RENVFTGSVSSLAWFMSAQETSFVTLAQRVLANPLKVRMHYGHPDVFDRFWFLTRGGISK 1260
            RENVFTGSVSSLAWFMSAQETSFVTLAQRVLANPLKVRMHYGHPDVFDRFWFLTRGGISK
Sbjct: 1201 RENVFTGSVSSLAWFMSAQETSFVTLAQRVLANPLKVRMHYGHPDVFDRFWFLTRGGISK 1260

Query: 1261 ASKVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGFNQISMFEAKVASGNGEQVLS 1320
            ASKVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGFNQISMFEAKVASGNGEQVLS
Sbjct: 1261 ASKVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGFNQISMFEAKVASGNGEQVLS 1320

Query: 1321 RDIYRLGHRLDFFRVLSVFYTTVGYYFNTMLVVLSVYTFLWGRLYLALSGVEDAAVASSS 1380
            RDIYRLGHRLDFFRVLSVFYTTVGYYFNTMLVVLSVY+FLWGRLYLALSGVEDAA+ASS+
Sbjct: 1321 RDIYRLGHRLDFFRVLSVFYTTVGYYFNTMLVVLSVYSFLWGRLYLALSGVEDAAIASST 1380

Query: 1381 GNNRALGAILNQQFIIQLGLFTALPMIVENSLEHGFLPAIWNFLTMQLQLASFFYTFSLG 1440
            GNNRALGAILNQQFIIQLGLFTALPMIVENSLEHGFLPA+WNFLTMQLQLASFFYTFSLG
Sbjct: 1381 GNNRALGAILNQQFIIQLGLFTALPMIVENSLEHGFLPAVWNFLTMQLQLASFFYTFSLG 1440

Query: 1441 TRTHFFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFVKAIELGVILIVYASRS 1500
            TRTHFFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFVKAIELGVILIVYASRS
Sbjct: 1441 TRTHFFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFVKAIELGVILIVYASRS 1500

Query: 1501 PLARSTFTFVILSISSWFLIVSWIMAPFIFNPSGFDWLKTVYDFDDFINWLWNAGGVFTK 1560
            PLA +TFTFVILSISSWFLIVSWIMAPFIFNPSGFDWLKTVYDFDDFI+WLWNAGGVFTK
Sbjct: 1501 PLATNTFTFVILSISSWFLIVSWIMAPFIFNPSGFDWLKTVYDFDDFISWLWNAGGVFTK 1560

Query: 1561 AEQSWEAWWLEENSHLRSTGLWGKLLEIILDLRFFFFQYAIVYHLNITGNNTSIAVYFIS 1620
            AEQSWEAWWLEENSHLRSTGLWGKLLEIILDLRFFFFQYAIVYHLNITGNNTSIAVYFIS
Sbjct: 1561 AEQSWEAWWLEENSHLRSTGLWGKLLEIILDLRFFFFQYAIVYHLNITGNNTSIAVYFIS 1620

Query: 1621 WVSMIVLVGIYIVIAYARDKYAAKEHIYYRLVQLIVIVITVLVIVILMEFTPFNTGDLVT 1680
            WVSMI LVGIYIV+AYARDKYAAKEHIYYRLVQLIVIVITVLVIVILMEFTPFN GDLVT
Sbjct: 1621 WVSMIALVGIYIVVAYARDKYAAKEHIYYRLVQLIVIVITVLVIVILMEFTPFNVGDLVT 1680

Query: 1681 CLLAFIPTGWGIISIAQVLRPFLQTTVVWDTVVSMARLYDLLFGVIAMAPLALLSWLPGF 1740
            CLLAFIPTGWGIISIAQVLRPFLQTTVVWDTVVS+ARLYDLLFG+IAMAPLALLSWLPGF
Sbjct: 1681 CLLAFIPTGWGIISIAQVLRPFLQTTVVWDTVVSLARLYDLLFGMIAMAPLALLSWLPGF 1740

Query: 1741 QSMQTRILFNEAFSRGLQISRIIAGKKTV 1770
            QSMQTRILFNEAFSRGLQISRIIAGKKTV
Sbjct: 1741 QSMQTRILFNEAFSRGLQISRIIAGKKTV 1769

BLAST of HG10021216 vs. NCBI nr
Match: XP_023001687.1 (callose synthase 11 [Cucurbita maxima] >XP_023001688.1 callose synthase 11 [Cucurbita maxima])

HSP 1 Score: 3342.4 bits (8665), Expect = 0.0e+00
Identity = 1661/1770 (93.84%), Postives = 1721/1770 (97.23%), Query Frame = 0

Query: 1    MNMRQRPQPAGRGGFPN-PPPPVEPYNIIPIHDLLTDHPSLQSPEVRAAAAALRTVGELR 60
            MN+RQRPQP GRGGF N PPPPVEPYNIIPIHDLLTDHPSLQSPEVRAAAAALRTVGELR
Sbjct: 1    MNLRQRPQPVGRGGFQNAPPPPVEPYNIIPIHDLLTDHPSLQSPEVRAAAAALRTVGELR 60

Query: 61   RPSFVPWNPKYDLLDWLGLFFGFQSDNVRNQREHLVLHLANSQMRLRSSPEQADLLDRTV 120
            RPSFVPWNPKYDLLDWLGLFFGFQ DNVRNQREHLVLHLANSQMRLRSSPE+ D LDRTV
Sbjct: 61   RPSFVPWNPKYDLLDWLGLFFGFQDDNVRNQREHLVLHLANSQMRLRSSPERPDELDRTV 120

Query: 121  LRNFRKKLLRSYTLWCSYLGRKSNVRFPRRDQSEERRELLYVSLYLLIWGEAANLRFVPE 180
            LRNFRKKLLR+YTLWCSYLGRKSNVR   RD+SE RRELLYVSLYLLIWGEAANLRF PE
Sbjct: 121  LRNFRKKLLRNYTLWCSYLGRKSNVRLSSRDKSEGRRELLYVSLYLLIWGEAANLRFAPE 180

Query: 181  CLCYIYHFMAMELNQILDDYIDPETGRPYSPAIHGDCAFLKSVVMPIYQTIKTEVESSRN 240
            CLCYIYHFMAMELNQILDDYIDP+TGRPYSPAIHGDCAFLKSVVMPIY+TIKTEVESSRN
Sbjct: 181  CLCYIYHFMAMELNQILDDYIDPDTGRPYSPAIHGDCAFLKSVVMPIYKTIKTEVESSRN 240

Query: 241  GTAPHSAWRNYDDINEYFWSRRCFHNLGWPLELSSNFFATTPKNRRVGKTGFVEQRSFWN 300
            G+APHSAWRNYDDINEYFWSRRCF +LGWPL LSSNFFATTPKNRRVGKTG+VEQRSFWN
Sbjct: 241  GSAPHSAWRNYDDINEYFWSRRCFRSLGWPLNLSSNFFATTPKNRRVGKTGYVEQRSFWN 300

Query: 301  IFRSFDKIWILLLLFLQASIIVAWQGHQYPWIALKSRDVQVNLLTVFITWSGLRLFQAVL 360
            IFRSFDKIWILLLLFLQASIIVAWQG QYPWIALKSRDVQV LLTVFITWSGLRLFQAVL
Sbjct: 301  IFRSFDKIWILLLLFLQASIIVAWQGQQYPWIALKSRDVQVELLTVFITWSGLRLFQAVL 360

Query: 361  DAGTQYSLVSKETMWLGVRMLLKGLAAIAWIIVFAVFYARIWSQKNSDGFWSDEANGKIF 420
            DAGTQYSLVS+ETMWLGVRMLLKGLAAIAWIIVF++FYARIWS+K SDGFWSDEANG+IF
Sbjct: 361  DAGTQYSLVSRETMWLGVRMLLKGLAAIAWIIVFSIFYARIWSRKKSDGFWSDEANGEIF 420

Query: 421  IFLRIVFAFVIPELLALLLFVLPWIRNGLEELDWKVMYLFTWWFHTRIFVGRGLREGLID 480
            IFLR VFAF+IPE+LALLLFVLPWIRNGLEELDWKV+Y+FTWWFHTR+F+GRGLREGLID
Sbjct: 421  IFLRAVFAFLIPEMLALLLFVLPWIRNGLEELDWKVLYIFTWWFHTRMFIGRGLREGLID 480

Query: 481  NIKYTIFWVAVLASKFSFSYFFQIQPLVGPTKGLLNLKGPYKWHEFFGSTNIVAVVLLWT 540
            NIKY+IFWVAVLA+KF+FSYFFQIQPLVGPTKGLLNLKGPYKWHEFFGSTN+VAVVLLWT
Sbjct: 481  NIKYSIFWVAVLAAKFAFSYFFQIQPLVGPTKGLLNLKGPYKWHEFFGSTNVVAVVLLWT 540

Query: 541  PVVLIYLMDLQIWYSIFSSFVGAIVGLFLHLGEIRNIGQLRLRFQFFASAMQFNLMPEEQ 600
            PVVLIYLMDLQIWY+IFSS VGA VGLFLHLGEIRNIGQL LRFQFFASAMQF+LMPE Q
Sbjct: 541  PVVLIYLMDLQIWYTIFSSLVGATVGLFLHLGEIRNIGQLSLRFQFFASAMQFSLMPEVQ 600

Query: 601  QLTPKLTRLKKIRDAIHRLKLRYGLGQPYKKIESSMIDTTRFALIWNEILITMREEDLIS 660
            QLTPK+TRLKKIRDAIHRLKLRYGLGQPYKKIESS IDTT+FALIWNEI+ITMREEDLIS
Sbjct: 601  QLTPKMTRLKKIRDAIHRLKLRYGLGQPYKKIESSRIDTTKFALIWNEIIITMREEDLIS 660

Query: 661  DRDFDLLELPPNYWNIRVIRWPCFLLCNELLLALSQAIELGNKPDEDLWSKICKNEYQRC 720
            DRDFDLLELPPN WNIRVIRWPCFLLCNELLLALSQA EL NKPDEDLWSKICKNEYQRC
Sbjct: 661  DRDFDLLELPPNCWNIRVIRWPCFLLCNELLLALSQATELANKPDEDLWSKICKNEYQRC 720

Query: 721  AVIEAYDSVKALLLNIVKYGSEENSIVVKVFIDIDNVIGMGKFMEAYNPNVLQEIHTKLI 780
            AVIEAYDSVKA+LL IVKYGSEENSIV K+F+DIDNVIG+GKF++AYN NVL EIHTKLI
Sbjct: 721  AVIEAYDSVKAVLLTIVKYGSEENSIVAKLFMDIDNVIGLGKFLDAYNSNVLPEIHTKLI 780

Query: 781  SLVELLIGTKKDLSKAVFILQALYELSIREFPRSKKSTKQLREEGLVPRNPATDEGLLFE 840
            SL++LLIGTKKDLSKAV ILQALYELSIREFPR KKSTKQLREEGLVPRNPATDEGLLF 
Sbjct: 781  SLLDLLIGTKKDLSKAVNILQALYELSIREFPRLKKSTKQLREEGLVPRNPATDEGLLFV 840

Query: 841  NAVVFPGLEDKYFYRNVQRLHTILTSRDSMHNVPSNLEARRRLAFFSNSLFMNMPRAPYV 900
             AV FP LEDK FYRNVQRLHTILTSRDSMH VPSNLEARRRLAFFSNSLFMN+PRAPYV
Sbjct: 841  KAVEFPDLEDKMFYRNVQRLHTILTSRDSMHGVPSNLEARRRLAFFSNSLFMNIPRAPYV 900

Query: 901  EKMMPFSVLTPYYDEEVVYGKEMLRSENEDGVSTLFYLQRIYEDEWRNFMERMRKKGLEH 960
            EKMMPFSVLTPYYDEEVVYGKEMLRSENEDGVSTLFYLQRIYEDEWRNFMERMRK+GLEH
Sbjct: 901  EKMMPFSVLTPYYDEEVVYGKEMLRSENEDGVSTLFYLQRIYEDEWRNFMERMRKEGLEH 960

Query: 961  EDDIWTKKSRELRLWASYRGQTLSRTVRGMMYYHRALKMFSFLDTASEMDIRMGSQEIAS 1020
            EDDIWTKKSR+LRLWASYRGQTLSRTVRGMMYYHRALKMFSFLDTASE+DIRMGSQEIAS
Sbjct: 961  EDDIWTKKSRDLRLWASYRGQTLSRTVRGMMYYHRALKMFSFLDTASEVDIRMGSQEIAS 1020

Query: 1021 HGSITRKHALDGLHSTQPPSRNLNRASTGELLYRRSEYGTALMKFTYVVTCQVYGLQKAK 1080
            HGSITRKHALDGL STQPP RNLNR+++GELL+R  EYGTALMKFTYVVTCQVYGLQKAK
Sbjct: 1021 HGSITRKHALDGLRSTQPPFRNLNRSTSGELLFRGYEYGTALMKFTYVVTCQVYGLQKAK 1080

Query: 1081 RDPRAEEILNLMKDNESLRVAYVDEVHRGRDEVEFYSVLVKYDQELKKEVVIYRIKLPGP 1140
            RD RAEEILNLMK+NE+LRVAYVDEVHRGRDEVEFYSVLVKYDQEL+KEVVIYRI+LPGP
Sbjct: 1081 RDSRAEEILNLMKENEALRVAYVDEVHRGRDEVEFYSVLVKYDQELQKEVVIYRIRLPGP 1140

Query: 1141 LKIGEGKPENQNHAIIFTRGDAIQTIDMNQDNYFEEALKMRNLLEEFNNSYGIRKPTILG 1200
            LKIGEGKPENQNHAIIFTRGDAIQTIDMNQ+NYFEEALKMRNLLEEF NSYGIRKPTILG
Sbjct: 1141 LKIGEGKPENQNHAIIFTRGDAIQTIDMNQENYFEEALKMRNLLEEFKNSYGIRKPTILG 1200

Query: 1201 VRENVFTGSVSSLAWFMSAQETSFVTLAQRVLANPLKVRMHYGHPDVFDRFWFLTRGGIS 1260
            VRENVFTGSVSSLAWFMSAQETSFVTLAQRVLANPLKVRMHYGHPDVFDRFWFLTRGGIS
Sbjct: 1201 VRENVFTGSVSSLAWFMSAQETSFVTLAQRVLANPLKVRMHYGHPDVFDRFWFLTRGGIS 1260

Query: 1261 KASKVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGFNQISMFEAKVASGNGEQVL 1320
            KAS+VINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGFNQISMFEAKVASGNGEQVL
Sbjct: 1261 KASRVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGFNQISMFEAKVASGNGEQVL 1320

Query: 1321 SRDIYRLGHRLDFFRVLSVFYTTVGYYFNTMLVVLSVYTFLWGRLYLALSGVEDAAVASS 1380
            SRD+YRLGHRLDFFR+LSVFYTTVGYYFNTMLVVLSV+ FLWGRLYLALSGVEDAAVASS
Sbjct: 1321 SRDVYRLGHRLDFFRMLSVFYTTVGYYFNTMLVVLSVFVFLWGRLYLALSGVEDAAVASS 1380

Query: 1381 SGNNRALGAILNQQFIIQLGLFTALPMIVENSLEHGFLPAIWNFLTMQLQLASFFYTFSL 1440
            SGNNRALGAILNQQFIIQLGLFTALPMIVENSLEHGFLPAIWNFLTMQLQLASFFYTFSL
Sbjct: 1381 SGNNRALGAILNQQFIIQLGLFTALPMIVENSLEHGFLPAIWNFLTMQLQLASFFYTFSL 1440

Query: 1441 GTRTHFFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFVKAIELGVILIVYASR 1500
            GTRTHFFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFVKAIELGVILIVYASR
Sbjct: 1441 GTRTHFFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFVKAIELGVILIVYASR 1500

Query: 1501 SPLARSTFTFVILSISSWFLIVSWIMAPFIFNPSGFDWLKTVYDFDDFINWLWNAGGVFT 1560
            SPLA+STFTFVILSISSWFLIVSWIMAPFIFNPSGFDWLKTVYDFDDFINWLWN GGVFT
Sbjct: 1501 SPLAKSTFTFVILSISSWFLIVSWIMAPFIFNPSGFDWLKTVYDFDDFINWLWNTGGVFT 1560

Query: 1561 KAEQSWEAWWLEENSHLRSTGLWGKLLEIILDLRFFFFQYAIVYHLNITGNNTSIAVYFI 1620
            KAEQSWEAWWLEENSHLR+TGLWGKLLEIILDLRFFFFQYAIVYHLNITGNNTSIAVYFI
Sbjct: 1561 KAEQSWEAWWLEENSHLRTTGLWGKLLEIILDLRFFFFQYAIVYHLNITGNNTSIAVYFI 1620

Query: 1621 SWVSMIVLVGIYIVIAYARDKYAAKEHIYYRLVQLIVIVITVLVIVILMEFTPFNTGDLV 1680
            SWVSMIVLVGIYIVIAYA+DKYAAKEHIYYRLVQLIVIV+ VLVIVILMEFTPFN GDLV
Sbjct: 1621 SWVSMIVLVGIYIVIAYAQDKYAAKEHIYYRLVQLIVIVVIVLVIVILMEFTPFNMGDLV 1680

Query: 1681 TCLLAFIPTGWGIISIAQVLRPFLQTTVVWDTVVSMARLYDLLFGVIAMAPLALLSWLPG 1740
            TCLLAFIPTGWGIISIAQVLRPFLQTTVVWDTVVS ARLYDLLFG+I MAPLALLSWLPG
Sbjct: 1681 TCLLAFIPTGWGIISIAQVLRPFLQTTVVWDTVVSSARLYDLLFGMIVMAPLALLSWLPG 1740

Query: 1741 FQSMQTRILFNEAFSRGLQISRIIAGKKTV 1770
            FQSMQTRILFNEAFSRGLQISRIIAGKKT+
Sbjct: 1741 FQSMQTRILFNEAFSRGLQISRIIAGKKTI 1770

BLAST of HG10021216 vs. ExPASy Swiss-Prot
Match: Q9S9U0 (Callose synthase 11 OS=Arabidopsis thaliana OX=3702 GN=CALS11 PE=2 SV=1)

HSP 1 Score: 2533.8 bits (6566), Expect = 0.0e+00
Identity = 1259/1774 (70.97%), Postives = 1487/1774 (83.82%), Query Frame = 0

Query: 4    RQRPQPAGRGGFPNPPPPVEPYNIIPIHDLLTDHPSLQSPEVRAAAAALRTVGELRRPSF 63
            RQRP  A         P +E YNIIPIHD LT+HPSL+ PEVRAAAAALR VG+L +P F
Sbjct: 3    RQRPSVA----TARDAPSLEVYNIIPIHDFLTEHPSLRYPEVRAAAAALRIVGDLPKPPF 62

Query: 64   VPWNPKYDLLDWLGLFFGFQSDNVRNQREHLVLHLANSQMRLRSSPEQADLLDRTVLRNF 123
              + P+ DL+DWLGL FGFQ DNVRNQRE+LVLHLANSQMRL+  P   D LD TVLR F
Sbjct: 63   ADFTPRMDLMDWLGLLFGFQIDNVRNQRENLVLHLANSQMRLQPPPRHPDGLDPTVLRRF 122

Query: 124  RKKLLRSYTLWCSYLGRKSNVRFPRRDQSEE------RRELLYVSLYLLIWGEAANLRFV 183
            RKKLLR+YT WCS+LG + +V  P + + +       RRELLYV+LYLLIWGE+ANLRF+
Sbjct: 123  RKKLLRNYTNWCSFLGVRCHVTSPIQSRHQTNAVLNLRRELLYVALYLLIWGESANLRFM 182

Query: 184  PECLCYIYHFMAMELNQILDDYIDPETGRPYSPAIHGDCAFLKSVVMPIYQTIKTEVESS 243
            PECLCYI+H MAMELN++L    D  TG PY P+  GDCAFLKSVVMPIY+T+KTEVESS
Sbjct: 183  PECLCYIFHHMAMELNKVLAGEFDDMTGMPYWPSFSGDCAFLKSVVMPIYKTVKTEVESS 242

Query: 244  RNGTAPHSAWRNYDDINEYFWSRRCFHNLGWPLELSSNFFATTPKNRRVGKTGFVEQRSF 303
             NGT PHSAWRNYDDINEYFWS+R   +L WPL+ +SNFF TTPK+ RVGKTGFVEQRSF
Sbjct: 243  NNGTKPHSAWRNYDDINEYFWSKRALKSLKWPLDYTSNFFDTTPKSSRVGKTGFVEQRSF 302

Query: 304  WNIFRSFDKIWILLLLFLQASIIVAWQGHQYPWIALKSRDVQVNLLTVFITWSGLRLFQA 363
            WN++RSFD++WILLLL+LQA+IIVA    ++PW   + RDV+V LLTVFI+W+GLRL Q+
Sbjct: 303  WNVYRSFDRLWILLLLYLQAAIIVATSDVKFPW---QDRDVEVALLTVFISWAGLRLLQS 362

Query: 364  VLDAGTQYSLVSKETMWLGVRMLLKGLAAIAWIIVFAVFYARIWSQKNSDGFWSDEANGK 423
            VLDA TQYSLVS+ET WL +R+ LK + A+AW ++F+VFYARIWSQKN DG WS  AN +
Sbjct: 363  VLDASTQYSLVSRETYWLFIRLTLKFVVAVAWTVLFSVFYARIWSQKNKDGVWSRAANER 422

Query: 424  IFIFLRIVFAFVIPELLALLLFVLPWIRNGLEELDWKVMYLFTWWFHTRIFVGRGLREGL 483
            +  FL++VF +VIPELLAL+LF++P IRN +EEL+  V+Y  TWWF+++ FVGRG+REGL
Sbjct: 423  VVTFLKVVFVYVIPELLALVLFIVPCIRNWVEELNLGVVYFLTWWFYSKTFVGRGMREGL 482

Query: 484  IDNIKYTIFWVAVLASKFSFSYFFQIQPLVGPTKGLLNLK-GPYKWHEFFGSTNIVAVVL 543
            +DN+KYT+FW+ VLA+KF FSYF QI+PL+ PT+ LLNLK   Y WHEFFGST+ +AV +
Sbjct: 483  VDNVKYTLFWIIVLATKFIFSYFLQIRPLIAPTRALLNLKDATYNWHEFFGSTHRIAVGM 542

Query: 544  LWTPVVLIYLMDLQIWYSIFSSFVGAIVGLFLHLGEIRNIGQLRLRFQFFASAMQFNLMP 603
            LW PV+L+YLMDLQIWYSI+SS VGA +GLF HLGEIRNI QLRLRFQFF+SAMQFNL P
Sbjct: 543  LWLPVILVYLMDLQIWYSIYSSLVGATIGLFSHLGEIRNIDQLRLRFQFFSSAMQFNLKP 602

Query: 604  EEQQLTPKLTRLKKIRDAIHRLKLRYGLGQPYKKIESSMIDTTRFALIWNEILITMREED 663
            EE  L+PK T LKK RDAIHRLKLRYG+GQP+ KIESS ++ T FALIWNEI++T REED
Sbjct: 603  EEHLLSPKATMLKKARDAIHRLKLRYGIGQPFNKIESSQVEATWFALIWNEIILTFREED 662

Query: 664  LISDRDFDLLELPPNYWNIRVIRWPCFLLCNELLLALSQAIELGNKPDEDLWSKICKNEY 723
            LISDR+ +LLELPPN WNIRVIRWPCFLLCNELLLALSQA EL + PD  LWSKIC +EY
Sbjct: 663  LISDREVELLELPPNCWNIRVIRWPCFLLCNELLLALSQANELCDAPDHWLWSKICSSEY 722

Query: 724  QRCAVIEAYDSVKALLLNIVKYGSEENSIVVKVFIDIDNVIGMGKFMEAYNPNVLQEIHT 783
            +RCAV+EA+DS+K ++L IVK G+EE SI+ ++F++ID  +   K  E Y   VL  IH 
Sbjct: 723  RRCAVMEAFDSIKFVILKIVKNGTEEESILNRLFMEIDENVENEKITEVYKLTVLLRIHE 782

Query: 784  KLISLVELLIGTKKDLSKAVFILQALYELSIREFPRSKKSTKQLREEGLVPRNPATDEGL 843
            KLISL+E L+  +K + + V ILQALYEL   EFP++++ST QLR+ GL P +   D  L
Sbjct: 783  KLISLLERLMDPEKKVFRIVNILQALYELCAWEFPKTRRSTPQLRQLGLAPISLEADTEL 842

Query: 844  LFENAVVFPGLEDKYFYRNVQRLHTILTSRDSMHNVPSNLEARRRLAFFSNSLFMNMPRA 903
            LF NA+  P L+D  FYR ++R+HTILTSRD MHNVP N+EAR RLAFFSNSLFM MP+A
Sbjct: 843  LFVNAINLPPLDDVVFYRQIRRVHTILTSRDPMHNVPKNIEARERLAFFSNSLFMTMPQA 902

Query: 904  PYVEKMMPFSVLTPYYDEEVVYGKEMLRSENEDGVSTLFYLQRIYEDEWRNFMERMRKKG 963
            P VEKMM FSVLTPYYDEEV+Y +EMLR+ENEDG+STLFYLQRIYEDEW NF+ERMR++G
Sbjct: 903  PSVEKMMAFSVLTPYYDEEVMYRQEMLRAENEDGISTLFYLQRIYEDEWVNFLERMRREG 962

Query: 964  LEHEDDIWTKKSRELRLWASYRGQTLSRTVRGMMYYHRALKMFSFLDTASEMDIRMGSQE 1023
             E+E+DIW+KK R+LRLWASYRGQTLSRTVRGMMYY+ ALK  +FLD+ASEMDIRMG+Q 
Sbjct: 963  AENENDIWSKKVRDLRLWASYRGQTLSRTVRGMMYYYSALKKLAFLDSASEMDIRMGTQ- 1022

Query: 1024 IASHGSITRKHALDGLHSTQP-PSRNLNRASTG-ELLYRRSEYGTALMKFTYVVTCQVYG 1083
            IA     +      G ++ QP PS+ ++R ++G   L + SEYG+A+MKFTYVV CQVYG
Sbjct: 1023 IAPEARRSYYTNDGGDNTLQPTPSQEISRMASGITHLLKGSEYGSAMMKFTYVVACQVYG 1082

Query: 1084 LQKAKRDPRAEEILNLMKDNESLRVAYVDEVHRGRDEVEFYSVLVKYDQELKKEVVIYRI 1143
              KA+ D RAEEIL LMK++++LR+AYVDEV  GR EVE+YSVLVK+DQ+L++EV IYRI
Sbjct: 1083 QHKARGDHRAEEILFLMKNHDALRIAYVDEVDLGRGEVEYYSVLVKFDQQLQREVEIYRI 1142

Query: 1144 KLPGPLKIGEGKPENQNHAIIFTRGDAIQTIDMNQDNYFEEALKMRNLLEEFNNSYGIRK 1203
            +LPGPLK+GEGKPENQNHA+IFTRGDAIQTIDMNQDN+FEEALKMRNLLE F   YGIRK
Sbjct: 1143 RLPGPLKLGEGKPENQNHALIFTRGDAIQTIDMNQDNHFEEALKMRNLLESFKTYYGIRK 1202

Query: 1204 PTILGVRENVFTGSVSSLAWFMSAQETSFVTLAQRVLANPLKVRMHYGHPDVFDRFWFLT 1263
            PTILGVRE VFTGSVSSLAWFMSAQETSFVTL QRVLANPLKVRMHYGHPDVFDRFWF+ 
Sbjct: 1203 PTILGVREKVFTGSVSSLAWFMSAQETSFVTLGQRVLANPLKVRMHYGHPDVFDRFWFVP 1262

Query: 1264 RGGISKASKVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGFNQISMFEAKVASGN 1323
            RGGISKAS+VINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVG NQISMFEAKVASGN
Sbjct: 1263 RGGISKASRVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGLNQISMFEAKVASGN 1322

Query: 1324 GEQVLSRDIYRLGHRLDFFRVLSVFYTTVGYYFNTMLVVLSVYTFLWGRLYLALSGVEDA 1383
            GEQ LSRD+YRLGHRLDFFR+LS FYTTVGYYFNTML+V +VY FLWGRLYLALSGVE  
Sbjct: 1323 GEQALSRDVYRLGHRLDFFRMLSFFYTTVGYYFNTMLIVFTVYAFLWGRLYLALSGVEKI 1382

Query: 1384 AVASSSGNNRALGAILNQQFIIQLGLFTALPMIVENSLEHGFLPAIWNFLTMQLQLASFF 1443
            A   SS +N ALGAILNQQFIIQLGLFTALPMI+ENSLE GFLPA+W+F+TMQLQLASFF
Sbjct: 1383 AKDRSS-SNEALGAILNQQFIIQLGLFTALPMILENSLERGFLPAVWDFITMQLQLASFF 1442

Query: 1444 YTFSLGTRTHFFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFVKAIELGVILI 1503
            YTFS+GTRTH+FGRTILHGGAKYRATGRGFVV+HK FAENYRLYAR+HF+KAIEL +IL+
Sbjct: 1443 YTFSMGTRTHYFGRTILHGGAKYRATGRGFVVEHKKFAENYRLYARTHFIKAIELAIILL 1502

Query: 1504 VYASRSPLARSTFTFVILSISSWFLIVSWIMAPFIFNPSGFDWLKTVYDFDDFINWLWNA 1563
            VYA+ SPLA+S+F +++++ISSWFLI SWI++PF+FNPSGFDWLKTV DFDDFI WLW+ 
Sbjct: 1503 VYAAYSPLAKSSFVYILMTISSWFLITSWIISPFLFNPSGFDWLKTVNDFDDFIAWLWSR 1562

Query: 1564 GGVFTKAEQSWEAWWLEENSHLRSTGLWGKLLEIILDLRFFFFQYAIVYHLNITGNNTSI 1623
            GG+FTKA+QSW  WW EE  HL++TG+WGKLLEIILDLRFFFFQY+IVYHL I  N TSI
Sbjct: 1563 GGLFTKADQSWFTWWNEEQEHLKTTGVWGKLLEIILDLRFFFFQYSIVYHLRIAENRTSI 1622

Query: 1624 AVYFISWVSMIVLVGIYIVIAYARDKYAAKEHIYYRLVQLIVIVITVLVIVILMEFTPFN 1683
             VY ISW  +I +V IYI   YA+ +Y+ KEHI YR +Q +VI++TVLV+V++++FT   
Sbjct: 1623 GVYLISWGCIIGIVAIYITTIYAQKRYSVKEHIKYRFIQFLVILLTVLVVVMMLQFTKLT 1682

Query: 1684 TGDLVTCLLAFIPTGWGIISIAQVLRPFLQTTVVWDTVVSMARLYDLLFGVIAMAPLALL 1743
              DL+  LLAF+PTGWG+ISIAQVL+PFL +TVVWDTV+S+AR YDL FG+I MAP+ALL
Sbjct: 1683 VVDLLISLLAFVPTGWGLISIAQVLKPFLLSTVVWDTVISVARFYDLFFGLIVMAPVALL 1742

Query: 1744 SWLPGFQSMQTRILFNEAFSRGLQISRIIAGKKT 1769
            SWLPGFQ+MQTRILFNEAFSRGLQIS I+AGKK+
Sbjct: 1743 SWLPGFQNMQTRILFNEAFSRGLQISIILAGKKS 1767

BLAST of HG10021216 vs. ExPASy Swiss-Prot
Match: Q9ZT82 (Callose synthase 12 OS=Arabidopsis thaliana OX=3702 GN=CALS12 PE=2 SV=1)

HSP 1 Score: 2381.7 bits (6171), Expect = 0.0e+00
Identity = 1168/1757 (66.48%), Postives = 1431/1757 (81.45%), Query Frame = 0

Query: 23   EPYNIIPIHDLLTDHPSLQSPEVRAAAAALRTVGELRRPSFVPWNPKYDLLDWLGLFFGF 82
            EPYNIIP+++LL DHPSL+ PEVRAAAAAL+TVG+LRRP +V W   YDLLDWL LFFGF
Sbjct: 26   EPYNIIPVNNLLADHPSLRFPEVRAAAAALKTVGDLRRPPYVQWRSHYDLLDWLALFFGF 85

Query: 83   QSDNVRNQREHLVLHLANSQMRLRSSPEQADLLDRTVLRNFRKKLLRSYTLWCSYLGRKS 142
            Q DNVRNQREH+VLHLAN+QMRL   P+  D LD  V+R FR+KLL +Y+ WCSYLG+KS
Sbjct: 86   QKDNVRNQREHMVLHLANAQMRLSPPPDNIDSLDSAVVRRFRRKLLANYSSWCSYLGKKS 145

Query: 143  NVRFPRRDQSEERRELLYVSLYLLIWGEAANLRFVPECLCYIYHFMAMELNQILDDYIDP 202
            N+    R+  + RRELLYV LYLLIWGEAANLRF+PEC+CYI+H MA ELN+IL+D +D 
Sbjct: 146  NIWISDRN-PDSRRELLYVGLYLLIWGEAANLRFMPECICYIFHNMASELNKILEDCLDE 205

Query: 203  ETGRPYSPAIHGDCAFLKSVVMPIYQTIKTEVESSRNGTAPHSAWRNYDDINEYFWSRRC 262
             TG+PY P++ G+ AFL  VV PIY TI+ E++ S+NGT  H  WRNYDDINEYFW+ RC
Sbjct: 206  NTGQPYLPSLSGENAFLTGVVKPIYDTIQAEIDESKNGTVAHCKWRNYDDINEYFWTDRC 265

Query: 263  FHNLGWPLELSSNFFATTPKNRRVGKTGFVEQRSFWNIFRSFDKIWILLLLFLQASIIVA 322
            F  L WPL+L SNFF +  + + VGKTGFVE+R+F+ ++RSFD++W++L LFLQA+IIVA
Sbjct: 266  FSKLKWPLDLGSNFFKS--RGKSVGKTGFVERRTFFYLYRSFDRLWVMLALFLQAAIIVA 325

Query: 323  WQGH-------QYPWIALKSRDVQVNLLTVFITWSGLRLFQAVLDAGTQYSLVSKETMWL 382
            W+         +  W ALK+RDVQV LLTVF+TWSG+RL QAVLDA +QY LVS+ET   
Sbjct: 326  WEEKPDTSSVTRQLWNALKARDVQVRLLTVFLTWSGMRLLQAVLDAASQYPLVSRETKRH 385

Query: 383  GVRMLLKGLAAIAWIIVFAVFYARIWSQKNSDGFWSDEANGKIFIFLRIVFAFVIPELLA 442
              RML+K +AA  WI+ F V Y  IW QK  D  WS+ A  KI+ FL  V AF++PE+LA
Sbjct: 386  FFRMLMKVIAAAVWIVAFTVLYTNIWKQKRQDRQWSNAATTKIYQFLYAVGAFLVPEILA 445

Query: 443  LLLFVLPWIRNGLEELDWKVMYLFTWWFHTRIFVGRGLREGLIDNIKYTIFWVAVLASKF 502
            L LF++PW+RN LEE +WK+ +  TWWF  + FVGRGLREGL+DNIKY+ FW+ VLA+KF
Sbjct: 446  LALFIIPWMRNFLEETNWKIFFALTWWFQGKSFVGRGLREGLVDNIKYSTFWIFVLATKF 505

Query: 503  SFSYFFQIQPLVGPTKGLLNLKG-PYKWHEFFGSTNIVAVVLLWTPVVLIYLMDLQIWYS 562
            +FSYF Q++P++ P+K L NLK   Y+WH+F+G +N  +V LLW PVVLIYLMD+QIWY+
Sbjct: 506  TFSYFLQVKPMIKPSKLLWNLKDVDYEWHQFYGDSNRFSVALLWLPVVLIYLMDIQIWYA 565

Query: 563  IFSSFVGAIVGLFLHLGEIRNIGQLRLRFQFFASAMQFNLMPEEQQLTPKLTRLKKIRDA 622
            I+SS VGA+VGLF HLGEIR++GQLRLRFQFFASA+QFNLMPEEQ L  +     K +D 
Sbjct: 566  IYSSIVGAVVGLFDHLGEIRDMGQLRLRFQFFASAIQFNLMPEEQLLNARGFG-NKFKDG 625

Query: 623  IHRLKLRYGLGQPYKKIESSMIDTTRFALIWNEILITMREEDLISDRDFDLLELPPNYWN 682
            IHRLKLRYG G+P+KK+ES+ ++  +FALIWNEI++  REED++SDR+ +LLELP N W+
Sbjct: 626  IHRLKLRYGFGRPFKKLESNQVEANKFALIWNEIILAFREEDIVSDREVELLELPKNSWD 685

Query: 683  IRVIRWPCFLLCNELLLALSQAIELGNKPDEDLWSKICKNEYQRCAVIEAYDSVKALLLN 742
            + VIRWPCFLLCNELLLALSQA EL + PD+ LW KICKNEY+RCAV+EAYDS+K LLL+
Sbjct: 686  VTVIRWPCFLLCNELLLALSQARELIDAPDKWLWHKICKNEYRRCAVVEAYDSIKHLLLS 745

Query: 743  IVKYGSEENSIVVKVFIDIDNVIGMGKFMEAYNPNVLQEIHTKLISLVELLIGTKKDLSK 802
            I+K  +EE+SI+   F  I+  I   +F + +  ++L +I+  L  LV L+   + D  +
Sbjct: 746  IIKVDTEEHSIITVFFQIINQSIQSEQFTKTFRVDLLPKIYETLQKLVGLVNDEETDSGR 805

Query: 803  AVFILQALYELSIREFPRSKKSTKQLREEGLVPRNPATDEGLLFENAVVFPGLEDKYFYR 862
             V +LQ+LYE++ R+F   KK+T+QL  EGL PR+PA+   LLF+NA+  P   ++ FYR
Sbjct: 806  VVNVLQSLYEIATRQFFIEKKTTEQLSNEGLTPRDPASK--LLFQNAIRLPDASNEDFYR 865

Query: 863  NVQRLHTILTSRDSMHNVPSNLEARRRLAFFSNSLFMNMPRAPYVEKMMPFSVLTPYYDE 922
             V+RLHTILTSRDSMH+VP NLEARRR+AFFSNSLFMNMP AP VEKMM FSVLTPYY E
Sbjct: 866  QVRRLHTILTSRDSMHSVPVNLEARRRIAFFSNSLFMNMPHAPQVEKMMAFSVLTPYYSE 925

Query: 923  EVVYGKEMLRSENEDGVSTLFYLQRIYEDEWRNFMERMRKKGLEHEDDIWTKKSRELRLW 982
            EVVY KE LR+E EDG+STL+YLQ IY DEW+NF ERM ++G++ + ++WT K R+LRLW
Sbjct: 926  EVVYSKEQLRNETEDGISTLYYLQTIYADEWKNFKERMHREGIKTDSELWTTKLRDLRLW 985

Query: 983  ASYRGQTLSRTVRGMMYYHRALKMFSFLDTASEMDIRMGSQEIASHGSITRK--HALDGL 1042
            ASYRGQTL+RTVRGMMYY+RALKM +FLD+ASEMDIR G+QE+ S  ++  +     DG 
Sbjct: 986  ASYRGQTLARTVRGMMYYYRALKMLAFLDSASEMDIREGAQELGSVRNLQGELGGQSDGF 1045

Query: 1043 HSTQPPSRNLNRASTGELLYRRSEYGTALMKFTYVVTCQVYGLQKAKRDPRAEEILNLMK 1102
             S    S     +S+   LY+  EYGTALMKFTYVV CQ+YG QKAK++P+AEEIL LMK
Sbjct: 1046 VSENDRSSLSRASSSVSTLYKGHEYGTALMKFTYVVACQIYGSQKAKKEPQAEEILYLMK 1105

Query: 1103 DNESLRVAYVDEVHRGRDEVEFYSVLVKYDQELKKEVVIYRIKLPGPLKIGEGKPENQNH 1162
             NE+LR+AYVDEV  GR E ++YSVLVKYD +L+KEV I+R+KLPGP+K+GEGKPENQNH
Sbjct: 1106 QNEALRIAYVDEVPAGRGETDYYSVLVKYDHQLEKEVEIFRVKLPGPVKLGEGKPENQNH 1165

Query: 1163 AIIFTRGDAIQTIDMNQDNYFEEALKMRNLLEEFNNSYGIRKPTILGVRENVFTGSVSSL 1222
            A+IFTRGDA+QTIDMNQD+YFEEALKMRNLL+E+N+ +GIRKPTILGVRE++FTGSVSSL
Sbjct: 1166 AMIFTRGDAVQTIDMNQDSYFEEALKMRNLLQEYNHYHGIRKPTILGVREHIFTGSVSSL 1225

Query: 1223 AWFMSAQETSFVTLAQRVLANPLKVRMHYGHPDVFDRFWFLTRGGISKASKVINISEDIF 1282
            AWFMSAQETSFVTL QRVLANPLKVRMHYGHPDVFDRFWFL+RGGISKAS+VINISEDIF
Sbjct: 1226 AWFMSAQETSFVTLGQRVLANPLKVRMHYGHPDVFDRFWFLSRGGISKASRVINISEDIF 1285

Query: 1283 AGFNCTLRGGNVTHHEYIQVGKGRDVGFNQISMFEAKVASGNGEQVLSRDIYRLGHRLDF 1342
            AGFNCTLRGGNVTHHEYIQVGKGRDVG NQISMFEAKVASGNGEQVLSRD+YRLGHRLDF
Sbjct: 1286 AGFNCTLRGGNVTHHEYIQVGKGRDVGLNQISMFEAKVASGNGEQVLSRDVYRLGHRLDF 1345

Query: 1343 FRVLSVFYTTVGYYFNTMLVVLSVYTFLWGRLYLALSGVEDAAVASSSGNNRALGAILNQ 1402
            FR+LS FYTTVG++FNTM+V+L+VY FLWGR+YLALSGVE +A+A S+  N ALG ILNQ
Sbjct: 1346 FRMLSFFYTTVGFFFNTMMVILTVYAFLWGRVYLALSGVEKSALADSTDTNAALGVILNQ 1405

Query: 1403 QFIIQLGLFTALPMIVENSLEHGFLPAIWNFLTMQLQLASFFYTFSLGTRTHFFGRTILH 1462
            QFIIQLGLFTALPMIVE SLE GFL AIWNF+ MQ+QL++ FYTFS+GTR H+FGRTILH
Sbjct: 1406 QFIIQLGLFTALPMIVEWSLEEGFLLAIWNFIRMQIQLSAVFYTFSMGTRAHYFGRTILH 1465

Query: 1463 GGAKYRATGRGFVVQHKSFAENYRLYARSHFVKAIELGVILIVYASRSPLARSTFTFVIL 1522
            GGAKYRATGRGFVV+HK F ENYRLYARSHFVKAIELG+ILIVYAS SP+A+ +  ++ +
Sbjct: 1466 GGAKYRATGRGFVVEHKGFTENYRLYARSHFVKAIELGLILIVYASHSPIAKDSLIYIAM 1525

Query: 1523 SISSWFLIVSWIMAPFIFNPSGFDWLKTVYDFDDFINWLWNAGGVFTKAEQSWEAWWLEE 1582
            +I+SWFL++SWIMAPF+FNPSGFDWLKTVYDF+DF+NW+W  G + TK+EQSWE WW EE
Sbjct: 1526 TITSWFLVISWIMAPFVFNPSGFDWLKTVYDFEDFMNWIWYQGRISTKSEQSWEKWWYEE 1585

Query: 1583 NSHLRSTGLWGKLLEIILDLRFFFFQYAIVYHLNITGNNTSIAVYFISWVSMIVLVGIYI 1642
              HLR+TG  G  +EIIL LRFFFFQY IVY L I   +TS+ VY  SW+ +  +  +++
Sbjct: 1586 QDHLRNTGKAGLFVEIILVLRFFFFQYGIVYQLKIANGSTSLFVYLFSWIYIFAIFVLFL 1645

Query: 1643 VIAYARDKYAAKEHIYYRLVQLIVIVITVLVIVILMEFTPFNTGDLVTCLLAFIPTGWGI 1702
            VI YARDKY+AK HI YRLVQ ++IV+ +LVIV L+EFT F+  D+ T LLAFIPTGWGI
Sbjct: 1646 VIQYARDKYSAKAHIRYRLVQFLLIVLAILVIVALLEFTHFSFIDIFTSLLAFIPTGWGI 1705

Query: 1703 ISIAQVLRPFLQT-TVVWDTVVSMARLYDLLFGVIAMAPLALLSWLPGFQSMQTRILFNE 1762
            + IAQ  R +L+  T+ W+ VVS+AR+YD+LFG++ M P+A LSW+PGFQSMQTRILFNE
Sbjct: 1706 LLIAQTQRKWLKNYTIFWNAVVSVARMYDILFGILIMVPVAFLSWMPGFQSMQTRILFNE 1765

Query: 1763 AFSRGLQISRIIAGKKT 1769
            AFSRGL+I +I+ GKK+
Sbjct: 1766 AFSRGLRIMQIVTGKKS 1776

BLAST of HG10021216 vs. ExPASy Swiss-Prot
Match: Q3B724 (Callose synthase 5 OS=Arabidopsis thaliana OX=3702 GN=CALS5 PE=1 SV=1)

HSP 1 Score: 1546.6 bits (4003), Expect = 0.0e+00
Identity = 845/1787 (47.29%), Postives = 1159/1787 (64.86%), Query Frame = 0

Query: 24   PYNIIPIHDLLTDHPSLQSPEVRAAAAALRTVGELRRPSFVPWNPK----YDLLDWLGLF 83
            PYNI+P+         +Q  EV+AA AAL     L  PS    + K     DLLDWL   
Sbjct: 188  PYNILPLDSAGASQSVMQLEEVKAAVAALGNTRGLNWPSGFEQHRKKTGNLDLLDWLRAM 247

Query: 84   FGFQSDNVRNQREHLVLHLANSQMRLRSSPEQADLLDRTVLRNFRKKLLRSYTLWCSYLG 143
            FGFQ DNVRNQREHLV   A++ +RL   PE  + LD   +     KL ++Y  WC +LG
Sbjct: 248  FGFQRDNVRNQREHLVCLFADNHIRLTPKPEPLNKLDDRAVDTVMSKLFKNYKNWCKFLG 307

Query: 144  RKSNVRFPRRDQSEERRELLYVSLYLLIWGEAANLRFVPECLCYIYHFMAMELNQILDDY 203
            RK ++R P+  Q  ++R++LY+ LYLLIWGEAAN+RF+PECLCYI+H MA EL+ +L   
Sbjct: 308  RKHSLRLPQAAQDIQQRKILYMGLYLLIWGEAANIRFMPECLCYIFHNMAYELHGLLAGN 367

Query: 204  IDPETGRPYSPAIHGDC-AFLKSVVMPIYQTIKTEVESSRNGTAPHSAWRNYDDINEYFW 263
            +   TG    P+  GD  AFL+ V+ PIY+ ++TE   + NG A HS W NYDD+NEYFW
Sbjct: 368  VSIVTGENIKPSYGGDDEAFLRKVITPIYRVVQTEANKNANGKAAHSDWSNYDDLNEYFW 427

Query: 264  SRRCFHNLGWPLELSSNFFATT-----------PKNRRVGKTGFVEQRSFWNIFRSFDKI 323
            +  CF +LGWP+    + F +T            K  R GK+ F E R+FW+I+ SFD++
Sbjct: 428  TPDCF-SLGWPMRDDGDLFKSTRDTTQGKKGSFRKAGRTGKSNFTETRTFWHIYHSFDRL 487

Query: 324  WILLLLFLQASIIVAWQGHQYPWIALKSRDVQVNLLTVFITWSGLRLFQAVLDAGTQYSL 383
            W   LL LQA II+A++  +   I  K  DV   L ++FIT + LR  Q+VLD    +  
Sbjct: 488  WTFYLLALQAMIILAFERVELREILRK--DVLYALSSIFITAAFLRFLQSVLDVILNFPG 547

Query: 384  VSKETMWLGVRMLLKGLAAIAWIIVFAVFYARIWS----QKNSDGFWSDEANGKIFIFLR 443
              +      +R +LK + ++AW +V  + YA+  S    +      +  +  G   +++ 
Sbjct: 548  FHRWKFTDVLRNILKIVVSLAWCVVLPLCYAQSVSFAPGKLKQWLSFLPQVKGVPPLYIM 607

Query: 444  IVFAFVIPELLALLLFVLPWIRNGLEELDWKVMYLFTWWFHTRIFVGRGLREGLIDNIKY 503
             V  +++P +LA ++F+ P +R  +E  DW +  L  WW   RI+VGRG+ E  I  IKY
Sbjct: 608  AVALYLLPNVLAAIMFIFPMLRRWIENSDWHIFRLLLWWSQPRIYVGRGMHESQIALIKY 667

Query: 504  TIFWVAVLASKFSFSYFFQIQPLVGPTKGLLNLKG-PYKWHEFFGST--NIVAVVLLWTP 563
            TIFW+ +   KF+FSYF Q++ LV PT  +++++   YKWHEFF +   N  AVV LW P
Sbjct: 668  TIFWLLLFCCKFAFSYFLQVKLLVKPTNAIMSIRHVKYKWHEFFPNAEHNYGAVVSLWLP 727

Query: 564  VVLIYLMDLQIWYSIFSSFVGAIVGLFLHLGEIRNIGQLRLRFQFFASAMQFNLMPEEQQ 623
            V+L+Y MD QIWY+IFS+  G ++G F  LGEIR +G LR RFQ    A    L+P +  
Sbjct: 728  VILVYFMDTQIWYAIFSTICGGVIGAFDRLGEIRTLGMLRSRFQSLPGAFNTYLVPSD-- 787

Query: 624  LTPKLTRLKKIRDAIHRLKLRYGLGQPYKKIESSMIDTTRFALIWNEILITMREEDLISD 683
                     K R     L  R+      ++ E++     +F+ +WNEI+ + REEDLISD
Sbjct: 788  ---------KTRRRGFSLSKRFAEVTAARRTEAA-----KFSQLWNEIISSFREEDLISD 847

Query: 684  RDFDLLELP-PNYWNIRVIRWPCFLLCNELLLALSQAIELGNKPDEDLWSKICKNEYQRC 743
            R+ DLL +P  +  ++++I+WP FLL +++ +AL  A +   + D DLW +IC +EY +C
Sbjct: 848  REMDLLLVPYTSDPSLKLIQWPPFLLASKIPIALDMAAQFRTR-DSDLWKRICADEYMKC 907

Query: 744  AVIEAYDSVKALLLNIVKYGSEENSIVVKVFIDIDNVIGMGKFMEAYNPNVLQEIHTKLI 803
            AVIE Y+S K +L  +V  G  E  I+  +  ++++ I    F+  +    L  + +K +
Sbjct: 908  AVIECYESFKHVLHTLV-IGENEKRIIGIIIKEVESNISKNSFLSNFRMAPLPALCSKFV 967

Query: 804  SLVELLIG---TKKDLSKAVFILQALYELSIREFPRSKKSTKQLREEGLVPRNPATD--E 863
             LV +L      K+D    V +LQ + E+  R+  +++   ++L E G   +        
Sbjct: 968  ELVGILKNADPAKRD--TVVLLLQDMLEVVTRDMMQNE--NRELVELGHTNKESGRQLFA 1027

Query: 864  GLLFENAVVFPGLEDKYFYRNVQRLHTILTSRDSMHNVPSNLEARRRLAFFSNSLFMNMP 923
            G   + A++FP +    ++  + RLH +LT ++S  +VP+NLEA+RR+AFF+NSLFM+MP
Sbjct: 1028 GTDAKPAILFPPVATAQWHEQISRLHLLLTVKESAMDVPTNLEAQRRIAFFTNSLFMDMP 1087

Query: 924  RAPYVEKMMPFSVLTPYYDEEVVYGKEMLRSENEDGVSTLFYLQRIYEDEWRNFMERMRK 983
            RAP V  M+ FSVLTPYY EE VY K  L  ENEDGVS ++YLQ+I+ DEW NF+ER+  
Sbjct: 1088 RAPRVRNMLSFSVLTPYYSEETVYSKNDLEMENEDGVSVVYYLQKIFPDEWTNFLERLDC 1147

Query: 984  KG----LEHEDDIWTKKSRELRLWASYRGQTLSRTVRGMMYYHRALKMFSFLDTASEMDI 1043
            K     LE E++I      +LR W S RGQTL RTVRGMMYY RALK+ +FLD A+E +I
Sbjct: 1148 KDETSVLESEENI-----LQLRHWVSLRGQTLFRTVRGMMYYRRALKLQAFLDMANETEI 1207

Query: 1044 RMGSQEIASHGSITRKHALDGLHSTQPPSRNLNRASTGELLYRRSEYGTALMKFTYVVTC 1103
              G + I+      +K                    +   LY + E   A +KFTYV TC
Sbjct: 1208 LAGYKAISEPTEEDKK--------------------SQRSLYTQLE-AVADLKFTYVATC 1267

Query: 1104 QVYGLQKAKRDPRAEEILNLMKDNESLRVAYVDEVHR---GRDEVEFYSVLVKYDQELKK 1163
            Q YG QK   D RA +ILNLM +N SLRVAY+DEV     G+ +  FYSVL+K    L +
Sbjct: 1268 QNYGNQKRSGDRRATDILNLMVNNPSLRVAYIDEVEEREGGKVQKVFYSVLIKAVDNLDQ 1327

Query: 1164 EVVIYRIKLPGPLKIGEGKPENQNHAIIFTRGDAIQTIDMNQDNYFEEALKMRNLLEEFN 1223
            E  IYRIKLPGP KIGEGKPENQNHA+IFTRG+A+Q IDMNQD+Y EEALKMRNLLEEFN
Sbjct: 1328 E--IYRIKLPGPAKIGEGKPENQNHALIFTRGEALQAIDMNQDHYLEEALKMRNLLEEFN 1387

Query: 1224 NSYGIRKPTILGVRENVFTGSVSSLAWFMSAQETSFVTLAQRVLANPLKVRMHYGHPDVF 1283
              +G+R PTILG RE++FTGSVSSLAWFMS QETSFVT+ QRVLA+PLKVR HYGHPDVF
Sbjct: 1388 EDHGVRAPTILGFREHIFTGSVSSLAWFMSNQETSFVTIGQRVLASPLKVRFHYGHPDVF 1447

Query: 1284 DRFWFLTRGGISKASKVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGFNQISMFE 1343
            DR + +TRGGISKAS+ IN+SEDIFAGFN TLR GNVTHHEYIQVGKGRDVG NQIS+FE
Sbjct: 1448 DRIFHITRGGISKASRGINLSEDIFAGFNSTLRRGNVTHHEYIQVGKGRDVGLNQISLFE 1507

Query: 1344 AKVASGNGEQVLSRDIYRLGHRLDFFRVLSVFYTTVGYYFNTMLVVLSVYTFLWGRLYLA 1403
            AKVA GNGEQ LSRD+YRLGHR DFFR++S ++TTVG+Y ++M+VVL+VY FL+GRLYL+
Sbjct: 1508 AKVACGNGEQTLSRDLYRLGHRFDFFRMMSCYFTTVGFYISSMIVVLTVYAFLYGRLYLS 1567

Query: 1404 LSGVEDAAV--ASSSGNNRALGAILNQQFIIQLGLFTALPMIVENSLEHGFLPAIWNFLT 1463
            LSGVE+A V  A++ G++ +L A +  Q ++QLGL   LPM++E  LE GF  A+ + + 
Sbjct: 1568 LSGVEEAIVKFAAAKGDS-SLKAAMASQSVVQLGLLMTLPMVMEIGLERGFRTALSDLII 1627

Query: 1464 MQLQLASFFYTFSLGTRTHFFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFVK 1523
            MQLQLA  F+TFSLGT+ H++GRTILHGG+KYRATGRGFVV+H+ FAENYR+Y+RSHFVK
Sbjct: 1628 MQLQLAPVFFTFSLGTKVHYYGRTILHGGSKYRATGRGFVVKHEKFAENYRMYSRSHFVK 1687

Query: 1524 AIELGVILIVYASRSPLARSTFTFVILSISSWFLIVSWIMAPFIFNPSGFDWLKTVYDFD 1583
             +EL V+LI Y      A  +  + ++  S+WFL+ SW+ APF FNPSGF+W K V D+D
Sbjct: 1688 GMELMVLLICYRIYGKAAEDSVGYALVMGSTWFLVGSWLFAPFFFNPSGFEWQKIVDDWD 1747

Query: 1584 DFINWLWNAGGVFTKAEQSWEAWWLEENSHLRSTGLWGKLLEIILDLRFFFFQYAIVYHL 1643
            D+  W+ + GG+   A +SWE+WW EE  HL  +G +GK  EI L LR+F +QY IVY L
Sbjct: 1748 DWNKWISSRGGIGVPANKSWESWWEEEQEHLLHSGFFGKFWEIFLSLRYFIYQYGIVYQL 1807

Query: 1644 NIT-----GNNTSIAVYFISWVSMIVLVGIYIVIAYARDKYAAKEHIYYRLVQLIVIVIT 1703
            N+T     G   SI VY +SW+ ++ ++ +  +++  R K++A   + +RL++L + + +
Sbjct: 1808 NLTKESRMGKQHSIIVYGLSWLVIVAVMIVLKIVSMGRKKFSADFQLMFRLLKLFLFIGS 1867

Query: 1704 VLVIVILMEFTPFNTGDLVTCLLAFIPTGWGIISIAQVLRPFLQTTVVWDTVVSMARLYD 1763
            V+++ +L  F     GD++  LLAF+PTGW ++ I+QV RP ++T  +W +V ++AR Y+
Sbjct: 1868 VVIVGMLFHFLKLTVGDIMQSLLAFLPTGWALLQISQVARPLMKTVGMWGSVKALARGYE 1920

Query: 1764 LLFGVIAMAPLALLSWLPGFQSMQTRILFNEAFSRGLQISRIIAGKK 1768
             + GV+   P+ +L+W P     QTR+LFN+AFSRGLQI RI+AG K
Sbjct: 1928 YIMGVVIFMPVTVLAWFPFVSEFQTRLLFNQAFSRGLQIQRILAGGK 1920

BLAST of HG10021216 vs. ExPASy Swiss-Prot
Match: Q9AUE0 (Callose synthase 1 OS=Arabidopsis thaliana OX=3702 GN=CALS1 PE=1 SV=2)

HSP 1 Score: 1541.2 bits (3989), Expect = 0.0e+00
Identity = 841/1805 (46.59%), Postives = 1150/1805 (63.71%), Query Frame = 0

Query: 24   PYNIIPIHDLLTDHPSLQSPEVRAAAAALRTVGELRRPSFVPWNPKY------DLLDWLG 83
            PYNI+P+     +   ++ PE++AA AALR    L      PW   +      D+LDWL 
Sbjct: 185  PYNILPLDPDSQNQAIMRLPEIQAAVAALRNTRGL------PWTAGHKKKLDEDILDWLQ 244

Query: 84   LFFGFQSDNVRNQREHLVLHLANSQMRLRSSPEQADLLDRTVLRNFRKKLLRSYTLWCSY 143
              FGFQ DNV NQREHL+L LAN  +R    P+Q   LD   L    KKL R+Y  WC Y
Sbjct: 245  SMFGFQKDNVLNQREHLILLLANVHIRQFPKPDQQPKLDDRALTIVMKKLFRNYKKWCKY 304

Query: 144  LGRKSNVRFPRRDQSEERRELLYVSLYLLIWGEAANLRFVPECLCYIYHFMAMELNQILD 203
            LGRKS++  P   Q  ++R+LLY+ LYLLIWGEAANLRF+PECLCYIYH MA EL  +L 
Sbjct: 305  LGRKSSLWLPTIQQEVQQRKLLYMGLYLLIWGEAANLRFMPECLCYIYHHMAFELYGMLA 364

Query: 204  DYIDPETGRPYSPAIHG-DCAFLKSVVMPIYQTIKTEVESSRNGTAPHSAWRNYDDINEY 263
              + P TG    PA  G D AFL+ VV PIYQTI  E + SR G + HS WRNYDD+NEY
Sbjct: 365  GSVSPMTGEHVKPAYGGEDEAFLQKVVTPIYQTISKEAKRSRGGKSKHSVWRNYDDLNEY 424

Query: 264  FWSRRCFHNLGWPLELSSNFFATTPK--------------NRRVGKTGFVEQRSFWNIFR 323
            FWS RCF  LGWP+   ++FF  T +              +R +GK  FVE RSFW+IFR
Sbjct: 425  FWSIRCF-RLGWPMRADADFFCQTAEELRLERSEIKSNSGDRWMGKVNFVEIRSFWHIFR 484

Query: 324  SFDKIWILLLLFLQASIIVAWQGHQYPWIALKSRDVQVNLLTVFITWSGLRLFQAVLDAG 383
            SFD++W   +L LQA I++AW G      A+   DV + +L+VFIT + L+L QAVLD  
Sbjct: 485  SFDRLWSFYILCLQAMIVIAWNG-SGELSAIFQGDVFLKVLSVFITAAILKLAQAVLDIA 544

Query: 384  TQYSLVSKETMWLGVRMLLKGLAAIAWIIVFAVFYARIWSQKNSDGF-------WSDEAN 443
              +      ++++ +R ++K  AA  W++V AV YA  W  KN+ GF       +   ++
Sbjct: 545  LSWKARHSMSLYVKLRYVMKVGAAAVWVVVMAVTYAYSW--KNASGFSQTIKNWFGGHSH 604

Query: 444  GKIFIFLRIVFAFVIPELLALLLFVLPWIRNGLEELDWKVMYLFTWWFHTRIFVGRGLRE 503
                +F+  +  ++ P +L+ LLF+ P+IR  LE  D+K+M L  WW   R+++GRG+ E
Sbjct: 605  NSPSLFIVAILIYLSPNMLSALLFLFPFIRRYLERSDYKIMMLMMWWSQPRLYIGRGMHE 664

Query: 504  GLIDNIKYTIFWVAVLASKFSFSYFFQIQPLVGPTKGLLNLK-GPYKWHEFF--GSTNIV 563
              +   KYT+FW+ +L SK +FSY+ +I+PLVGPTK ++ +    Y WHEFF     N+ 
Sbjct: 665  SALSLFKYTMFWIVLLISKLAFSYYAEIKPLVGPTKDIMRIHISVYSWHEFFPHAKNNLG 724

Query: 564  AVVLLWTPVVLIYLMDLQIWYSIFSSFVGAIVGLFLHLGEIRNIGQLRLRFQFFASAMQF 623
             V+ LW+PV+L+Y MD QIWY+I S+ VG + G F  LGEIR +G LR RFQ    A   
Sbjct: 725  VVIALWSPVILVYFMDTQIWYAIVSTLVGGLNGAFRRLGEIRTLGMLRSRFQSIPGAFND 784

Query: 624  NLMPEEQQLTPKLTRLKKIRDAIHRLKLRYGLGQPYKKIESSM-IDTTRFALIWNEILIT 683
             L+P++              D   + + R    + + ++ SS   +  RFA +WN+I+ +
Sbjct: 785  CLVPQDNS------------DDTKKKRFRATFSRKFDQLPSSKDKEAARFAQMWNKIISS 844

Query: 684  MREEDLISDRDFDLLELPPNYW---NIRVIRWPCFLLCNELLLALSQAIELGNKPDEDLW 743
             REEDLISDR+ +LL +P  YW   ++ +IRWP FLL +++ +AL  A +  N  D +L 
Sbjct: 845  FREEDLISDREMELLLVP--YWSDPDLDLIRWPPFLLASKIPIALDMAKD-SNGKDRELK 904

Query: 744  SKICKNEYQRCAVIEAYDSVKALLLNIVKYGSEENSIVVKVFIDIDNVIGMGKFMEAYNP 803
             ++  + Y  CAV E Y S K  L+N +  G  E  ++  +F  ID  I     +   N 
Sbjct: 905  KRLAVDSYMTCAVRECYASFKN-LINYLVVGEREGQVINDIFSKIDEHIEKETLITELNL 964

Query: 804  NVLQEIHTKLISLVE-LLIGTKKDLSKAVFILQALYELSIREFPRSK-KSTKQLREEGLV 863
            + L +++ + + L+E LL   ++D  + V +L  + EL  R+    +  S  +    G  
Sbjct: 965  SALPDLYGQFVRLIEYLLENREEDKDQIVIVLLNMLELVTRDIMEEEVPSLLETAHNGSY 1024

Query: 864  PR----NPATDEGLLFENAVVFPGLEDKYFYRNVQRLHTILTSRDSMHNVPSNLEARRRL 923
             +     P   +   F         + + +   ++RLH +LT ++S  +VPSNLEARRRL
Sbjct: 1025 VKYDVMTPLHQQRKYFSQLRFPVYSQTEAWKEKIKRLHLLLTVKESAMDVPSNLEARRRL 1084

Query: 924  AFFSNSLFMNMPRAPYVEKMMPFSVLTPYYDEEVVYGKEMLRSENEDGVSTLFYLQRIYE 983
             FFSNSLFM+MP AP +  M+ FSVLTPY+ E+V++    L  +NEDGVS LFYLQ+I+ 
Sbjct: 1085 TFFSNSLFMDMPPAPKIRNMLSFSVLTPYFSEDVLFSIFGLEQQNEDGVSILFYLQKIFP 1144

Query: 984  DEWRNFMERMRKKGLEHEDDIWTKKSRELRLWASYRGQTLSRTVRGMMYYHRALKMFSFL 1043
            DEW NF+ER+ K G E E         ELRLWASYRGQTL++TVRGMMYY +AL++ +FL
Sbjct: 1145 DEWTNFLERV-KCGNEEELRAREDLEEELRLWASYRGQTLTKTVRGMMYYRKALELQAFL 1204

Query: 1044 DTASEMDIRMGSQEIASHGSITRKHALDGLHSTQPPSRNLNRASTGELLYRRSEYGTALM 1103
            D A + ++  G + +     +T + A                + +G  L+ + +   A M
Sbjct: 1205 DMAKDEELLKGYKAL----ELTSEEA----------------SKSGGSLWAQCQ-ALADM 1264

Query: 1104 KFTYVVTCQVYGLQKAKRDPRAEEILNLMKDNESLRVAYVDEV-------HRGRDEVEFY 1163
            KFT+VV+CQ Y + K   D RA++IL LM    S+RVAY+DEV       ++G +E  +Y
Sbjct: 1265 KFTFVVSCQQYSIHKRSGDQRAKDILRLMTTYPSIRVAYIDEVEQTHKESYKGTEEKIYY 1324

Query: 1164 SVLVKYDQELKK----------EVVIYRIKLPGPLKIGEGKPENQNHAIIFTRGDAIQTI 1223
            S LVK   + K           + +IYRIKLPGP  +GEGKPENQNHAIIFTRG+ +QTI
Sbjct: 1325 SALVKAAPQTKPMDSSESVQTLDQLIYRIKLPGPAILGEGKPENQNHAIIFTRGEGLQTI 1384

Query: 1224 DMNQDNYFEEALKMRNLLEEFNNSY-GIRKPTILGVRENVFTGSVSSLAWFMSAQETSFV 1283
            DMNQDNY EEA KMRNLL+EF   + G+R PTILG+RE++FTGSVSSLAWFMS QE SFV
Sbjct: 1385 DMNQDNYMEEAFKMRNLLQEFLEKHGGVRCPTILGLREHIFTGSVSSLAWFMSNQENSFV 1444

Query: 1284 TLAQRVLANPLKVRMHYGHPDVFDRFWFLTRGGISKASKVINISEDIFAGFNCTLRGGNV 1343
            T+ QRVLA+PLKVR HYGHPD+FDR + LTRGGI KASKVIN+SEDIFAGFN TLR GNV
Sbjct: 1445 TIGQRVLASPLKVRFHYGHPDIFDRLFHLTRGGICKASKVINLSEDIFAGFNSTLREGNV 1504

Query: 1344 THHEYIQVGKGRDVGFNQISMFEAKVASGNGEQVLSRDIYRLGHRLDFFRVLSVFYTTVG 1403
            THHEYIQVGKGRDVG NQISMFEAK+A+GNGEQ LSRD+YRLGHR DFFR+LS ++TT+G
Sbjct: 1505 THHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRDLYRLGHRFDFFRMLSCYFTTIG 1564

Query: 1404 YYFNTMLVVLSVYTFLWGRLYLALSGVEDAAVASSS-GNNRALGAILNQQFIIQLGLFTA 1463
            +YF+TML VL+VY FL+GRLYL LSG+E+   +  +  NN+ L A L  Q  +Q+G   A
Sbjct: 1565 FYFSTMLTVLTVYVFLYGRLYLVLSGLEEGLSSQRAFRNNKPLEAALASQSFVQIGFLMA 1624

Query: 1464 LPMIVENSLEHGFLPAIWNFLTMQLQLASFFYTFSLGTRTHFFGRTILHGGAKYRATGRG 1523
            LPM++E  LE GF  A+  F+ MQLQLAS F+TF LGT+TH++GRT+ HGGA+YR TGRG
Sbjct: 1625 LPMMMEIGLERGFHNALIEFVLMQLQLASVFFTFQLGTKTHYYGRTLFHGGAEYRGTGRG 1684

Query: 1524 FVVQHKSFAENYRLYARSHFVKAIELGVILIVYASRSPLARSTFTFVILSISSWFLIVSW 1583
            FVV H  FAENYR Y+RSHFVK IEL ++L+VY       R   T++++++S WF++V+W
Sbjct: 1685 FVVFHAKFAENYRFYSRSHFVKGIELMILLLVYQIFGQSYRGVVTYILITVSIWFMVVTW 1744

Query: 1584 IMAPFIFNPSGFDWLKTVYDFDDFINWLWNAGGVFTKAEQSWEAWWLEENSHLRSTGLWG 1643
            + APF+FNPSGF+W K V D+ D+  W++N GG+    E+SWE+WW +E  HLR +G+ G
Sbjct: 1745 LFAPFLFNPSGFEWQKIVDDWTDWNKWIYNRGGIGVPPEKSWESWWEKELEHLRHSGVRG 1804

Query: 1644 KLLEIILDLRFFFFQYAIVYHLN-ITGNNTSIAVYFISWVSMIVLVGIYIVIAYARDKYA 1703
              LEI L LRFF FQY +VYHL+   G N S  VY  SW  ++ ++ I   +   R +++
Sbjct: 1805 ITLEIFLALRFFIFQYGLVYHLSTFKGKNQSFWVYGASWFVILFILLIVKGLGVGRRRFS 1864

Query: 1704 AKEHIYYRLVQLIVIVITVLVIVILMEFTPFNTGDLVTCLLAFIPTGWGIISIAQVLRPF 1763
                + +R+++ +V +  V +++  +        DL  C+LAF+PTGWG++ IAQ  +P 
Sbjct: 1865 TNFQLLFRIIKGLVFLTFVAILITFLALPLITIKDLFICMLAFMPTGWGMLLIAQACKPL 1924

Query: 1764 LQTTVVWDTVVSMARLYDLLFGVIAMAPLALLSWLPGFQSMQTRILFNEAFSRGLQISRI 1768
            +Q   +W +V ++AR Y+++ G++   P+A L+W P     QTR+LFN+AFSRGLQISRI
Sbjct: 1925 IQQLGIWSSVRTLARGYEIVMGLLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRI 1941

BLAST of HG10021216 vs. ExPASy Swiss-Prot
Match: Q9LXT9 (Callose synthase 3 OS=Arabidopsis thaliana OX=3702 GN=CALS3 PE=3 SV=3)

HSP 1 Score: 1532.7 bits (3967), Expect = 0.0e+00
Identity = 832/1807 (46.04%), Postives = 1155/1807 (63.92%), Query Frame = 0

Query: 24   PYNIIPIHDLLTDHPSLQSPEVRAAAAALRTVGELRRPSFVPWNPKYDLLDWLGLFFGFQ 83
            PYNI+P+     +   ++ PE++AA  ALR    L  P         D+LDWL   FGFQ
Sbjct: 189  PYNILPLDPDSANQAIMRYPEIQAAVLALRNTRGLPWPEGHKKKKDEDMLDWLQEMFGFQ 248

Query: 84   SDNVRNQREHLVLHLANSQMRLRSSPEQADLLDRTVLRNFRKKLLRSYTLWCSYLGRKSN 143
             DNV NQREHL+L LAN  +R    P+Q   LD   L    KKL ++Y  WC YLGRKS+
Sbjct: 249  KDNVANQREHLILLLANVHIRQFPKPDQQPKLDDQALTEVMKKLFKNYKKWCKYLGRKSS 308

Query: 144  VRFPRRDQSEERRELLYVSLYLLIWGEAANLRFVPECLCYIYHFMAMELNQILDDYIDPE 203
            +  P   Q  ++R+LLY++LYLLIWGEAANLRF+PECLCYIYH MA EL  +L   + P 
Sbjct: 309  LWLPTIQQEMQQRKLLYMALYLLIWGEAANLRFMPECLCYIYHHMAFELYGMLAGNVSPM 368

Query: 204  TGRPYSPAIHG-DCAFLKSVVMPIYQTIKTEVESSRNGTAPHSAWRNYDDINEYFWSRRC 263
            TG    PA  G + AFL+ VV PIY+ I+ E + S+ G + HS WRNYDD+NEYFWS  C
Sbjct: 369  TGENVKPAYGGEEDAFLRKVVTPIYEVIQMEAQRSKKGKSKHSQWRNYDDLNEYFWSVDC 428

Query: 264  FHNLGWPLELSSNFFA-----------------TTPKNRRVGKTGFVEQRSFWNIFRSFD 323
            F  LGWP+   ++FF                     ++R VGK  FVE RSFW++FRSFD
Sbjct: 429  F-RLGWPMRADADFFCLPVAVPNTEKDGDNSKPIVARDRWVGKVNFVEIRSFWHVFRSFD 488

Query: 324  KIWILLLLFLQASIIVAWQGHQYPWIALKSRDVQVNLLTVFITWSGLRLFQAVLDAGTQY 383
            ++W   +L LQA II+AW G Q    ++   DV   +L+VFIT + ++L QAVLD    +
Sbjct: 489  RMWSFYILCLQAMIIMAWDGGQPS--SVFGADVFKKVLSVFITAAIMKLGQAVLDVILNF 548

Query: 384  SLVSKETMWLGVRMLLKGLAAIAWIIVFAVFYARIWSQKNSDGF------WSDEANGKIF 443
                  T+ + +R +LK  +A AW+I+  V YA  W  K+   F      W   A     
Sbjct: 549  KAHQSMTLHVKLRYILKVFSAAAWVIILPVTYAYSW--KDPPAFARTIKSWFGSAMHSPS 608

Query: 444  IFLRIVFAFVIPELLALLLFVLPWIRNGLEELDWKVMYLFTWWFHTRIFVGRGLREGLID 503
            +F+  V +++ P +LA ++F+ P +R  LE  +++++ L  WW   R++VGRG+ E    
Sbjct: 609  LFIIAVVSYLSPNMLAGVMFLFPLLRRFLERSNYRIVMLMMWWSQPRLYVGRGMHESAFS 668

Query: 504  NIKYTIFWVAVLASKFSFSYFFQIQPLVGPTKGLLNLK-GPYKWHEFF--GSTNIVAVVL 563
              KYT+FWV ++A+K +FSY+ +I+PLV PT+ ++  +   ++WHEFF     NI  V+ 
Sbjct: 669  LFKYTMFWVLLIATKLAFSYYIEIRPLVAPTQAIMKARVTNFQWHEFFPRAKNNIGVVIA 728

Query: 564  LWTPVVLIYLMDLQIWYSIFSSFVGAIVGLFLHLGEIRNIGQLRLRFQFFASAMQFNLMP 623
            LW P++L+Y MD QIWY+IFS+  G I G F  LGEIR +G LR RF+    A    L+P
Sbjct: 729  LWAPIILVYFMDSQIWYAIFSTLFGGIYGAFRRLGEIRTLGMLRSRFESLPGAFNDRLIP 788

Query: 624  EEQQLTPKLTRLKKIRDAIHRLKLRYGLGQPYKKIESSMIDTTRFALIWNEILITMREED 683
            + +    K    K I     R  L +   +    +     +  RFA +WN I+ + REED
Sbjct: 789  DGKNQQKK----KGI-----RATLSHNFTEDKVPVNKEK-EAARFAQLWNTIISSFREED 848

Query: 684  LISDRDFDLLELPPNYW---NIRVIRWPCFLLCNELLLALSQAIELGNKPDEDLWSKICK 743
            LISDR+ DLL +P  YW   ++ +I+WP FLL +++ +AL  A +  N  D +L  +I  
Sbjct: 849  LISDREMDLLLVP--YWADRDLDLIQWPPFLLASKIPIALDMAKD-SNGKDRELKKRIES 908

Query: 744  NEYQRCAVIEAYDSVKALLLNIVKYGSEENSIVVKVFIDIDNVIGMGKFMEAYNPNVLQE 803
            + Y +CAV E Y S K ++  +V+ G+ E  ++  +F ++D  I  G  ++ Y  + L  
Sbjct: 909  DTYMKCAVRECYASFKNIIKFVVQ-GNREKEVIEIIFAEVDKHIDTGDLIQEYKMSALPS 968

Query: 804  IHTKLISLVELLIGTK-KDLSKAVFILQALYELSIREFPRSKKSTKQLREE--------G 863
            ++   + L++ L+  K +D    V + Q + E+  R+      +   L +         G
Sbjct: 969  LYDHFVKLIKYLLDNKEEDRDHVVILFQDMLEVVTRDIMMEDYNISSLVDSSHGGTWHGG 1028

Query: 864  LVPRNPATDEGLLF--ENAVVFPGLE--DKYFYRNVQRLHTILTSRDSMHNVPSNLEARR 923
            ++   P   +  LF    A+ FP +E   + +   ++R++ +LT+++S  +VPSNLEARR
Sbjct: 1029 MI---PLEQQYQLFASSGAIRFP-IEPVTEAWKEKIKRIYLLLTTKESAMDVPSNLEARR 1088

Query: 924  RLAFFSNSLFMNMPRAPYVEKMMPFSVLTPYYDEEVVYGKEMLRSENEDGVSTLFYLQRI 983
            R++FFSNSLFM+MP AP V  M+ FSVLTPYY EEV++    L + NEDGVS LFYLQ+I
Sbjct: 1089 RISFFSNSLFMDMPMAPKVRNMLSFSVLTPYYTEEVLFSLRDLETPNEDGVSILFYLQKI 1148

Query: 984  YEDEWRNFMERMR---KKGLEHEDDIWTKKSRELRLWASYRGQTLSRTVRGMMYYHRALK 1043
            + DEW NF+ER++   ++ L+  D++      ELRLWASYRGQTL+RTVRGMMYY +AL+
Sbjct: 1149 FPDEWNNFLERVKCLSEEELKESDEL----EEELRLWASYRGQTLTRTVRGMMYYRKALE 1208

Query: 1044 MFSFLDTASEMDIRMGSQEIASHGSITRKHALDGLHSTQPPSRNLNRASTGELLYRRSEY 1103
            + +FLD A   D+                  ++G  + +  S N +R   GE        
Sbjct: 1209 LQAFLDMAMHEDL------------------MEGYKAVELNSENNSR---GERSLWAQCQ 1268

Query: 1104 GTALMKFTYVVTCQVYGLQKAKRDPRAEEILNLMKDNESLRVAYVDEVH--------RGR 1163
              A MKFTYVV+CQ YG+ K   DPRA++IL LM    SLRVAY+DEV         +G 
Sbjct: 1269 AVADMKFTYVVSCQQYGIHKRSGDPRAQDILRLMTRYPSLRVAYIDEVEEPVKDKSKKGN 1328

Query: 1164 DEVEFYSVLVKYDQEL-------KKEVVIYRIKLPGPLKIGEGKPENQNHAIIFTRGDAI 1223
             +V +YSVLVK  +           + VIYRI+LPGP  +GEGKPENQNHAIIF+RG+ +
Sbjct: 1329 QKV-YYSVLVKVPKSTDHSTLAQNLDQVIYRIRLPGPAILGEGKPENQNHAIIFSRGEGL 1388

Query: 1224 QTIDMNQDNYFEEALKMRNLLEEFNNSY-GIRKPTILGVRENVFTGSVSSLAWFMSAQET 1283
            QTIDMNQDNY EEALKMRNLL+EF   + G+R P+ILG+RE++FTGSVSSLAWFMS QET
Sbjct: 1389 QTIDMNQDNYMEEALKMRNLLQEFLTKHDGVRHPSILGLREHIFTGSVSSLAWFMSNQET 1448

Query: 1284 SFVTLAQRVLANPLKVRMHYGHPDVFDRFWFLTRGGISKASKVINISEDIFAGFNCTLRG 1343
            SFVT+ QR+LANPL+VR HYGHPDVFDR + LTRGG+SKASKVIN+SEDIFAGFN TLR 
Sbjct: 1449 SFVTIGQRLLANPLRVRFHYGHPDVFDRLFHLTRGGVSKASKVINLSEDIFAGFNSTLRE 1508

Query: 1344 GNVTHHEYIQVGKGRDVGFNQISMFEAKVASGNGEQVLSRDIYRLGHRLDFFRVLSVFYT 1403
            GNVTHHEYIQVGKGRDVG NQISMFEAK+A+GNGEQ LSRDIYRLGHR DFFR++S ++T
Sbjct: 1509 GNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRDIYRLGHRFDFFRMMSCYFT 1568

Query: 1404 TVGYYFNTMLVVLSVYTFLWGRLYLALSGVEDA-AVASSSGNNRALGAILNQQFIIQLGL 1463
            TVG+YF+T++ VL+VY FL+GRLYL LSG+E   +      +N  L   L  Q  +Q+G 
Sbjct: 1569 TVGFYFSTLITVLTVYIFLYGRLYLVLSGLEQGLSTQKGIRDNTPLQIALASQSFVQIGF 1628

Query: 1464 FTALPMIVENSLEHGFLPAIWNFLTMQLQLASFFYTFSLGTRTHFFGRTILHGGAKYRAT 1523
              ALPM++E  LE GF  A+  F+ MQLQLA  F+TFSLGT+TH++GRT+LHGGAKYR+T
Sbjct: 1629 LMALPMLMEIGLERGFRTALSEFVLMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAKYRST 1688

Query: 1524 GRGFVVQHKSFAENYRLYARSHFVKAIELGVILIVYASRSPLARSTFTFVILSISSWFLI 1583
            GRGFVV H  FA+NYRLY+RSHFVK +E+ ++L+VY       R    +++++IS WF++
Sbjct: 1689 GRGFVVFHAKFADNYRLYSRSHFVKGLEMMLLLVVYQIFGSAYRGVLAYLLITISMWFMV 1748

Query: 1584 VSWIMAPFIFNPSGFDWLKTVYDFDDFINWLWNAGGVFTKAEQSWEAWWLEENSHLRSTG 1643
             +W+ APF+FNPSGF+W K V D+ D+  W+ N GG+   AE+SWE+WW EE  HLR +G
Sbjct: 1749 GTWLFAPFLFNPSGFEWQKIVDDWTDWNKWINNIGGIGVPAEKSWESWWEEEQEHLRYSG 1808

Query: 1644 LWGKLLEIILDLRFFFFQYAIVYHLNITGNNTSIAVYFISWVSMIVLVGIYIVIAYARDK 1703
              G ++EI+L LRFF +QY +VYHL IT    +  VY +SW+ + +++ +   ++  R +
Sbjct: 1809 KRGIVVEILLALRFFIYQYGLVYHLTITEKTKNFLVYGVSWLVIFLILFVMKTVSVGRRR 1868

Query: 1704 YAAKEHIYYRLVQLIVIVITVLVIVILMEFTPFNTGDLVTCLLAFIPTGWGIISIAQVLR 1763
            ++A   + +RL++ ++ +  + +IVIL+        D++ C+LAF+PTGWG++ IAQ  +
Sbjct: 1869 FSASFQLMFRLIKGLIFMTFIAIIVILITLAHMTIQDIIVCILAFMPTGWGMLLIAQACK 1928

Query: 1764 PFLQTTVVWDTVVSMARLYDLLFGVIAMAPLALLSWLPGFQSMQTRILFNEAFSRGLQIS 1768
            P +     W +V ++AR Y+++ G++   P+A L+W P     QTR+LFN+AFSRGLQIS
Sbjct: 1929 PVVHRAGFWGSVRTLARGYEIVMGLLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQIS 1946

BLAST of HG10021216 vs. ExPASy TrEMBL
Match: A0A1S3B604 (1,3-beta-glucan synthase OS=Cucumis melo OX=3656 GN=LOC103486403 PE=3 SV=1)

HSP 1 Score: 3394.7 bits (8801), Expect = 0.0e+00
Identity = 1695/1769 (95.82%), Postives = 1728/1769 (97.68%), Query Frame = 0

Query: 1    MNMRQRPQPAGRGGFPNPPPPVEPYNIIPIHDLLTDHPSLQSPEVRAAAAALRTVGELRR 60
            MNMRQRPQ AGRGGFPNPPPPVEPYNIIPIHDLLTDHPSLQ PEVRAAAAALRTVGELRR
Sbjct: 1    MNMRQRPQAAGRGGFPNPPPPVEPYNIIPIHDLLTDHPSLQLPEVRAAAAALRTVGELRR 60

Query: 61   PSFVPWNPKYDLLDWLGLFFGFQSDNVRNQREHLVLHLANSQMRLRSSPEQADLLDRTVL 120
            PSFVPWNPKYDLLDWLGLFFGFQ+DNVRNQREHLVLHLANSQMRLRSSPEQ D+LDRTVL
Sbjct: 61   PSFVPWNPKYDLLDWLGLFFGFQNDNVRNQREHLVLHLANSQMRLRSSPEQPDVLDRTVL 120

Query: 121  RNFRKKLLRSYTLWCSYLGRKSNVRFPRRDQSEERRELLYVSLYLLIWGEAANLRFVPEC 180
            RNFRKKLLRSY+LWCSYLGRKSNVRFP RDQSEERRELLYVSLYLLIWGEAANLRF+PEC
Sbjct: 121  RNFRKKLLRSYSLWCSYLGRKSNVRFPSRDQSEERRELLYVSLYLLIWGEAANLRFLPEC 180

Query: 181  LCYIYHFMAMELNQILDDYIDPETGRPYSPAIHGDCAFLKSVVMPIYQTIKTEVESSRNG 240
            L YIYHFMAMELNQILDDYIDP+TGRPYSPAIHGDCAFLKSVVMPIYQTIK EVESSRNG
Sbjct: 181  LSYIYHFMAMELNQILDDYIDPDTGRPYSPAIHGDCAFLKSVVMPIYQTIKIEVESSRNG 240

Query: 241  TAPHSAWRNYDDINEYFWSRRCFHNLGWPLELSSNFFATTPKNRRVGKTGFVEQRSFWNI 300
            +APHSAWRNYDDINEYFWSRRCF +LGWPL LSSNFFATT KNRRVGKTGFVEQRSFWNI
Sbjct: 241  SAPHSAWRNYDDINEYFWSRRCFRSLGWPLNLSSNFFATTDKNRRVGKTGFVEQRSFWNI 300

Query: 301  FRSFDKIWILLLLFLQASIIVAWQGHQYPWIALKSRDVQVNLLTVFITWSGLRLFQAVLD 360
            FRSFDKIW+LLLLFLQASIIVAWQGHQYPWIALKSRDVQV LLTVFITWSG+R FQAVLD
Sbjct: 301  FRSFDKIWVLLLLFLQASIIVAWQGHQYPWIALKSRDVQVELLTVFITWSGMRFFQAVLD 360

Query: 361  AGTQYSLVSKETMWLGVRMLLKGLAAIAWIIVFAVFYARIWSQKNSDGFWSDEANGKIFI 420
            AGTQYSLVS+ETMWLGVRMLLKGLAA+AWIIVF+VFYARIW+QKNSDGFWSDEAN KIFI
Sbjct: 361  AGTQYSLVSRETMWLGVRMLLKGLAAVAWIIVFSVFYARIWNQKNSDGFWSDEANAKIFI 420

Query: 421  FLRIVFAFVIPELLALLLFVLPWIRNGLEELDWKVMYLFTWWFHTRIFVGRGLREGLIDN 480
            FLR VFAFVIPELLAL+ FVLPWIRNGLE LDWKVMYLFTWWFHTRIFVGRGLREGLIDN
Sbjct: 421  FLRAVFAFVIPELLALIFFVLPWIRNGLEGLDWKVMYLFTWWFHTRIFVGRGLREGLIDN 480

Query: 481  IKYTIFWVAVLASKFSFSYFFQIQPLVGPTKGLLNLKGPYKWHEFFGSTNIVAVVLLWTP 540
            IKYTIFWVAVLASKFSFSYFFQIQPLVGPTKGLLNLKGPYKWHEFFGSTNIVAVVLLWTP
Sbjct: 481  IKYTIFWVAVLASKFSFSYFFQIQPLVGPTKGLLNLKGPYKWHEFFGSTNIVAVVLLWTP 540

Query: 541  VVLIYLMDLQIWYSIFSSFVGAIVGLFLHLGEIRNIGQLRLRFQFFASAMQFNLMPEEQQ 600
            VVLIYLMDLQIWYSIFSSFVGAIVGLFLHLGEIRNIGQLRLRFQFFASAMQFNLMPE QQ
Sbjct: 541  VVLIYLMDLQIWYSIFSSFVGAIVGLFLHLGEIRNIGQLRLRFQFFASAMQFNLMPEVQQ 600

Query: 601  LTPKLTRLKKIRDAIHRLKLRYGLGQPYKKIESSMIDTTRFALIWNEILITMREEDLISD 660
            LTPKLTRLKKIRDAIHRLKLRYGLG  YKKIESS IDTT+FALIWNEILITMREEDLISD
Sbjct: 601  LTPKLTRLKKIRDAIHRLKLRYGLGLSYKKIESSRIDTTKFALIWNEILITMREEDLISD 660

Query: 661  RDFDLLELPPNYWNIRVIRWPCFLLCNELLLALSQAIELGNKPDEDLWSKICKNEYQRCA 720
            RDFDLLELPPNYW+IRVIRWPC LLCNELLLALSQA EL NKPDEDLW KICKNEYQRCA
Sbjct: 661  RDFDLLELPPNYWSIRVIRWPCVLLCNELLLALSQATELANKPDEDLWLKICKNEYQRCA 720

Query: 721  VIEAYDSVKALLLNIVKYGSEENSIVVKVFIDIDNVIGMGKFMEAYNPNVLQEIHTKLIS 780
            VIEAYDSVKALLL+IVKYGSEENSIVVK+FID+DN IG+GKFMEAYNPNVL EIH KLIS
Sbjct: 721  VIEAYDSVKALLLSIVKYGSEENSIVVKIFIDLDNAIGLGKFMEAYNPNVLPEIHAKLIS 780

Query: 781  LVELLIGTKKDLSKAVFILQALYELSIREFPRSKKSTKQLREEGLVPRNPATDEGLLFEN 840
            LVELLIGTKKDLSKAV ILQALYELSIREFPRSKKSTKQLREEGLVPRNPAT E  LFEN
Sbjct: 781  LVELLIGTKKDLSKAVDILQALYELSIREFPRSKKSTKQLREEGLVPRNPATYEEFLFEN 840

Query: 841  AVVFPGLEDKYFYRNVQRLHTILTSRDSMHNVPSNLEARRRLAFFSNSLFMNMPRAPYVE 900
            AVVFP +EDK+F RNVQRLHTILTSRDSMHNVPSNLEARRR+AFFSNSLFMNMPRAPYVE
Sbjct: 841  AVVFPSVEDKFFNRNVQRLHTILTSRDSMHNVPSNLEARRRIAFFSNSLFMNMPRAPYVE 900

Query: 901  KMMPFSVLTPYYDEEVVYGKEMLRSENEDGVSTLFYLQRIYEDEWRNFMERMRKKGLEHE 960
            KMMPFSVLTPYYDEEVVYGKEMLRSENEDGVSTLFYLQRIYEDEWRNFMERMRK+GLEHE
Sbjct: 901  KMMPFSVLTPYYDEEVVYGKEMLRSENEDGVSTLFYLQRIYEDEWRNFMERMRKEGLEHE 960

Query: 961  DDIWTKKSRELRLWASYRGQTLSRTVRGMMYYHRALKMFSFLDTASEMDIRMGSQEIASH 1020
            DDIWTKKSR+LRLWASYRGQTLSRTVRGMMYYHRALKMFSFLDTASEMDIRMGSQEIASH
Sbjct: 961  DDIWTKKSRDLRLWASYRGQTLSRTVRGMMYYHRALKMFSFLDTASEMDIRMGSQEIASH 1020

Query: 1021 GSITRKHALDGLHSTQPPSRNLNRASTGELLYRRSEYGTALMKFTYVVTCQVYGLQKAKR 1080
            GSITRKHA DGLHSTQP SR+LNRASTGE L+RRSEYGTALMKFTYVVTCQVYGLQKAKR
Sbjct: 1021 GSITRKHASDGLHSTQPASRDLNRASTGEWLHRRSEYGTALMKFTYVVTCQVYGLQKAKR 1080

Query: 1081 DPRAEEILNLMKDNESLRVAYVDEVHRGRDEVEFYSVLVKYDQELKKEVVIYRIKLPGPL 1140
            DPRAEEILNLMKDNESLRVAYVDEVH GRDEVEFYSVLVKYDQEL+KEVVIYRIKLPGPL
Sbjct: 1081 DPRAEEILNLMKDNESLRVAYVDEVHHGRDEVEFYSVLVKYDQELQKEVVIYRIKLPGPL 1140

Query: 1141 KIGEGKPENQNHAIIFTRGDAIQTIDMNQDNYFEEALKMRNLLEEFNNSYGIRKPTILGV 1200
            KIGEGKPENQNHAIIFTRGDAIQTIDMNQDNYFEEALKMRNLLEEFN SYGIRKPTILGV
Sbjct: 1141 KIGEGKPENQNHAIIFTRGDAIQTIDMNQDNYFEEALKMRNLLEEFNKSYGIRKPTILGV 1200

Query: 1201 RENVFTGSVSSLAWFMSAQETSFVTLAQRVLANPLKVRMHYGHPDVFDRFWFLTRGGISK 1260
            RENVFTGSVSSLAWFMSAQETSFVTLAQRVLANPLKVRMHYGHPDVFDRFWFLTRGGISK
Sbjct: 1201 RENVFTGSVSSLAWFMSAQETSFVTLAQRVLANPLKVRMHYGHPDVFDRFWFLTRGGISK 1260

Query: 1261 ASKVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGFNQISMFEAKVASGNGEQVLS 1320
            ASKVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGFNQISMFEAKVASGNGEQVLS
Sbjct: 1261 ASKVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGFNQISMFEAKVASGNGEQVLS 1320

Query: 1321 RDIYRLGHRLDFFRVLSVFYTTVGYYFNTMLVVLSVYTFLWGRLYLALSGVEDAAVASSS 1380
            RDIYRLGHRLDFFRVLSVFYTTVGYYFNTMLVVLSVYTFLWGRLYLALSGVEDAA+ASS+
Sbjct: 1321 RDIYRLGHRLDFFRVLSVFYTTVGYYFNTMLVVLSVYTFLWGRLYLALSGVEDAAIASST 1380

Query: 1381 GNNRALGAILNQQFIIQLGLFTALPMIVENSLEHGFLPAIWNFLTMQLQLASFFYTFSLG 1440
            GNNRALGAILNQQFIIQLGLFTALPMIVENSLEHGFLPAIWNFLTMQLQLASFFYTFSLG
Sbjct: 1381 GNNRALGAILNQQFIIQLGLFTALPMIVENSLEHGFLPAIWNFLTMQLQLASFFYTFSLG 1440

Query: 1441 TRTHFFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFVKAIELGVILIVYASRS 1500
            TRTHFFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFVKAIELGVILIVYASRS
Sbjct: 1441 TRTHFFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFVKAIELGVILIVYASRS 1500

Query: 1501 PLARSTFTFVILSISSWFLIVSWIMAPFIFNPSGFDWLKTVYDFDDFINWLWNAGGVFTK 1560
            PLA STFTFVILSISSWFLIVSWIMAPFIFNPSGFDWLKTVYDFDDFINWLWNAGGVFTK
Sbjct: 1501 PLATSTFTFVILSISSWFLIVSWIMAPFIFNPSGFDWLKTVYDFDDFINWLWNAGGVFTK 1560

Query: 1561 AEQSWEAWWLEENSHLRSTGLWGKLLEIILDLRFFFFQYAIVYHLNITGNNTSIAVYFIS 1620
            AEQSWEAWWLEENSHLRSTGLWGKLLEIILDLRFFFFQYAIVYHLNITGNNTSIAVYFIS
Sbjct: 1561 AEQSWEAWWLEENSHLRSTGLWGKLLEIILDLRFFFFQYAIVYHLNITGNNTSIAVYFIS 1620

Query: 1621 WVSMIVLVGIYIVIAYARDKYAAKEHIYYRLVQLIVIVITVLVIVILMEFTPFNTGDLVT 1680
            WVSMI LVGIYIVIAYA+DKYAAKEHIYYRLVQLIVIVITVLVIVILMEFTPFN GDLVT
Sbjct: 1621 WVSMIALVGIYIVIAYAQDKYAAKEHIYYRLVQLIVIVITVLVIVILMEFTPFNVGDLVT 1680

Query: 1681 CLLAFIPTGWGIISIAQVLRPFLQTTVVWDTVVSMARLYDLLFGVIAMAPLALLSWLPGF 1740
            CLLAFIPTGWGIISIAQVLRPFLQTTVVWDTVVS+ARLYDLLFG+I MAPLALLSWLPGF
Sbjct: 1681 CLLAFIPTGWGIISIAQVLRPFLQTTVVWDTVVSLARLYDLLFGMIVMAPLALLSWLPGF 1740

Query: 1741 QSMQTRILFNEAFSRGLQISRIIAGKKTV 1770
            QSMQTRILFNEAFSRGLQISRIIAGKKTV
Sbjct: 1741 QSMQTRILFNEAFSRGLQISRIIAGKKTV 1769

BLAST of HG10021216 vs. ExPASy TrEMBL
Match: A0A5A7UTW6 (1,3-beta-glucan synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00810 PE=3 SV=1)

HSP 1 Score: 3384.3 bits (8774), Expect = 0.0e+00
Identity = 1690/1764 (95.80%), Postives = 1723/1764 (97.68%), Query Frame = 0

Query: 6    RPQPAGRGGFPNPPPPVEPYNIIPIHDLLTDHPSLQSPEVRAAAAALRTVGELRRPSFVP 65
            RPQ AGRGGFPNPPPPVEPYNIIPIHDLLTDHPSLQ PEVRAAAAALRTVGELRRPSFVP
Sbjct: 65   RPQAAGRGGFPNPPPPVEPYNIIPIHDLLTDHPSLQLPEVRAAAAALRTVGELRRPSFVP 124

Query: 66   WNPKYDLLDWLGLFFGFQSDNVRNQREHLVLHLANSQMRLRSSPEQADLLDRTVLRNFRK 125
            WNPKYDLLDWLGLFFGFQ+DNVRNQREHLVLHLANSQMRLRSSPEQ D+LDRTVLRNFRK
Sbjct: 125  WNPKYDLLDWLGLFFGFQNDNVRNQREHLVLHLANSQMRLRSSPEQPDVLDRTVLRNFRK 184

Query: 126  KLLRSYTLWCSYLGRKSNVRFPRRDQSEERRELLYVSLYLLIWGEAANLRFVPECLCYIY 185
            KLLRSY+LWCSYLGRKSNVRFP RDQSEERRELLYVSLYLLIWGEAANLRF+PECL YIY
Sbjct: 185  KLLRSYSLWCSYLGRKSNVRFPSRDQSEERRELLYVSLYLLIWGEAANLRFLPECLSYIY 244

Query: 186  HFMAMELNQILDDYIDPETGRPYSPAIHGDCAFLKSVVMPIYQTIKTEVESSRNGTAPHS 245
            HFMAMELNQILDDYIDP+TGRPYSPAIHGDCAFLKSVVMPIYQTIK EVESSRNG+APHS
Sbjct: 245  HFMAMELNQILDDYIDPDTGRPYSPAIHGDCAFLKSVVMPIYQTIKIEVESSRNGSAPHS 304

Query: 246  AWRNYDDINEYFWSRRCFHNLGWPLELSSNFFATTPKNRRVGKTGFVEQRSFWNIFRSFD 305
            AWRNYDDINEYFWSRRCF +LGWPL LSSNFFATT KNRRVGKTGFVEQRSFWNIFRSFD
Sbjct: 305  AWRNYDDINEYFWSRRCFRSLGWPLNLSSNFFATTDKNRRVGKTGFVEQRSFWNIFRSFD 364

Query: 306  KIWILLLLFLQASIIVAWQGHQYPWIALKSRDVQVNLLTVFITWSGLRLFQAVLDAGTQY 365
            KIW+LLLLFLQASIIVAWQGHQYPWIALKSRDVQV LLTVFITWSG+R FQAVLDAGTQY
Sbjct: 365  KIWVLLLLFLQASIIVAWQGHQYPWIALKSRDVQVELLTVFITWSGMRFFQAVLDAGTQY 424

Query: 366  SLVSKETMWLGVRMLLKGLAAIAWIIVFAVFYARIWSQKNSDGFWSDEANGKIFIFLRIV 425
            SLVS+ETMWLGVRMLLKGLAA+AWIIVF+VFYARIW+QKNSDGFWSDEAN KIFIFLR V
Sbjct: 425  SLVSRETMWLGVRMLLKGLAAVAWIIVFSVFYARIWNQKNSDGFWSDEANAKIFIFLRAV 484

Query: 426  FAFVIPELLALLLFVLPWIRNGLEELDWKVMYLFTWWFHTRIFVGRGLREGLIDNIKYTI 485
            FAFVIPELLAL+ FVLPWIRNGLE LDWKVMYLFTWWFHTRIFVGRGLREGLIDNIKYTI
Sbjct: 485  FAFVIPELLALIFFVLPWIRNGLEGLDWKVMYLFTWWFHTRIFVGRGLREGLIDNIKYTI 544

Query: 486  FWVAVLASKFSFSYFFQIQPLVGPTKGLLNLKGPYKWHEFFGSTNIVAVVLLWTPVVLIY 545
            FWVAVLASKFSFSYFFQIQPLVGPTKGLLNLKGPYKWHEFFGSTNIVAVVLLWTPVVLIY
Sbjct: 545  FWVAVLASKFSFSYFFQIQPLVGPTKGLLNLKGPYKWHEFFGSTNIVAVVLLWTPVVLIY 604

Query: 546  LMDLQIWYSIFSSFVGAIVGLFLHLGEIRNIGQLRLRFQFFASAMQFNLMPEEQQLTPKL 605
            LMDLQIWYSIFSSFVGAIVGLFLHLGEIRNIGQLRLRFQFFASAMQFNLMPE QQLTPKL
Sbjct: 605  LMDLQIWYSIFSSFVGAIVGLFLHLGEIRNIGQLRLRFQFFASAMQFNLMPEVQQLTPKL 664

Query: 606  TRLKKIRDAIHRLKLRYGLGQPYKKIESSMIDTTRFALIWNEILITMREEDLISDRDFDL 665
            TRLKKIRDAIHRLKLRYGLG  YKKIESS IDTT+FALIWNEILITMREEDLISDRDFDL
Sbjct: 665  TRLKKIRDAIHRLKLRYGLGLSYKKIESSRIDTTKFALIWNEILITMREEDLISDRDFDL 724

Query: 666  LELPPNYWNIRVIRWPCFLLCNELLLALSQAIELGNKPDEDLWSKICKNEYQRCAVIEAY 725
            LELPPNYW+IRVIRWPC LLCNELLLALSQA EL NKPDEDLW KICKNEYQRCAVIEAY
Sbjct: 725  LELPPNYWSIRVIRWPCVLLCNELLLALSQATELANKPDEDLWLKICKNEYQRCAVIEAY 784

Query: 726  DSVKALLLNIVKYGSEENSIVVKVFIDIDNVIGMGKFMEAYNPNVLQEIHTKLISLVELL 785
            DSVKALLL+IVKYGSEENSIVVK+FID+DN IG+GKFMEAYNPNVL EIH KLISLVELL
Sbjct: 785  DSVKALLLSIVKYGSEENSIVVKIFIDLDNAIGLGKFMEAYNPNVLPEIHAKLISLVELL 844

Query: 786  IGTKKDLSKAVFILQALYELSIREFPRSKKSTKQLREEGLVPRNPATDEGLLFENAVVFP 845
            IGTKKDLSKAV ILQALYELSIREFPRSKKSTKQLREEGLVPRNPAT E  LFENAVVFP
Sbjct: 845  IGTKKDLSKAVDILQALYELSIREFPRSKKSTKQLREEGLVPRNPATYEEFLFENAVVFP 904

Query: 846  GLEDKYFYRNVQRLHTILTSRDSMHNVPSNLEARRRLAFFSNSLFMNMPRAPYVEKMMPF 905
             +EDK+F RNVQRLHTILTSRDSMHNVPSNLEARRR+AFFSNSLFMNMPRAPYVEKMMPF
Sbjct: 905  SVEDKFFNRNVQRLHTILTSRDSMHNVPSNLEARRRIAFFSNSLFMNMPRAPYVEKMMPF 964

Query: 906  SVLTPYYDEEVVYGKEMLRSENEDGVSTLFYLQRIYEDEWRNFMERMRKKGLEHEDDIWT 965
            SVLTPYYDEEVVYGKEMLRSENEDGVSTLFYLQRIYEDEWRNFMERMRK+GLEHEDDIWT
Sbjct: 965  SVLTPYYDEEVVYGKEMLRSENEDGVSTLFYLQRIYEDEWRNFMERMRKEGLEHEDDIWT 1024

Query: 966  KKSRELRLWASYRGQTLSRTVRGMMYYHRALKMFSFLDTASEMDIRMGSQEIASHGSITR 1025
            KKSR+LRLWASYRGQTLSRTVRGMMYYHRALKMFSFLDTASEMDIRMGSQEIASHGSITR
Sbjct: 1025 KKSRDLRLWASYRGQTLSRTVRGMMYYHRALKMFSFLDTASEMDIRMGSQEIASHGSITR 1084

Query: 1026 KHALDGLHSTQPPSRNLNRASTGELLYRRSEYGTALMKFTYVVTCQVYGLQKAKRDPRAE 1085
            KHA DGLHSTQP SR+LNRASTGE L+RRSEYGTALMKFTYVVTCQVYGLQKAKRDPRAE
Sbjct: 1085 KHASDGLHSTQPASRDLNRASTGEWLHRRSEYGTALMKFTYVVTCQVYGLQKAKRDPRAE 1144

Query: 1086 EILNLMKDNESLRVAYVDEVHRGRDEVEFYSVLVKYDQELKKEVVIYRIKLPGPLKIGEG 1145
            EILNLMKDNESLRVAYVDEVH GRDEVEFYSVLVKYDQEL+KEVVIYRIKLPGPLKIGEG
Sbjct: 1145 EILNLMKDNESLRVAYVDEVHHGRDEVEFYSVLVKYDQELQKEVVIYRIKLPGPLKIGEG 1204

Query: 1146 KPENQNHAIIFTRGDAIQTIDMNQDNYFEEALKMRNLLEEFNNSYGIRKPTILGVRENVF 1205
            KPENQNHAIIFTRGDAIQTIDMNQDNYFEEALKMRNLLEEFN SYGIRKPTILGVRENVF
Sbjct: 1205 KPENQNHAIIFTRGDAIQTIDMNQDNYFEEALKMRNLLEEFNKSYGIRKPTILGVRENVF 1264

Query: 1206 TGSVSSLAWFMSAQETSFVTLAQRVLANPLKVRMHYGHPDVFDRFWFLTRGGISKASKVI 1265
            TGSVSSLAWFMSAQETSFVTLAQRVLANPLKVRMHYGHPDVFDRFWFLTRGGISKASKVI
Sbjct: 1265 TGSVSSLAWFMSAQETSFVTLAQRVLANPLKVRMHYGHPDVFDRFWFLTRGGISKASKVI 1324

Query: 1266 NISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGFNQISMFEAKVASGNGEQVLSRDIYR 1325
            NISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGFNQISMFEAKVASGNGEQVLSRDIYR
Sbjct: 1325 NISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGFNQISMFEAKVASGNGEQVLSRDIYR 1384

Query: 1326 LGHRLDFFRVLSVFYTTVGYYFNTMLVVLSVYTFLWGRLYLALSGVEDAAVASSSGNNRA 1385
            LGHRLDFFRVLSVFYTTVGYYFNTMLVVLSVYTFLWGRLYLALSGVEDAA+ASS+GNNRA
Sbjct: 1385 LGHRLDFFRVLSVFYTTVGYYFNTMLVVLSVYTFLWGRLYLALSGVEDAAIASSTGNNRA 1444

Query: 1386 LGAILNQQFIIQLGLFTALPMIVENSLEHGFLPAIWNFLTMQLQLASFFYTFSLGTRTHF 1445
            LGAILNQQFIIQLGLFTALPMIVENSLEHGFLPAIWNFLTMQLQLASFFYTFSLGTRTHF
Sbjct: 1445 LGAILNQQFIIQLGLFTALPMIVENSLEHGFLPAIWNFLTMQLQLASFFYTFSLGTRTHF 1504

Query: 1446 FGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFVKAIELGVILIVYASRSPLARS 1505
            FGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFVKAIELGVILIVYASRSPLA S
Sbjct: 1505 FGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFVKAIELGVILIVYASRSPLATS 1564

Query: 1506 TFTFVILSISSWFLIVSWIMAPFIFNPSGFDWLKTVYDFDDFINWLWNAGGVFTKAEQSW 1565
            TFTFVILSISSWFLIVSWIMAPFIFNPSGFDWLKTVYDFDDFINWLWNAGGVFTKAEQSW
Sbjct: 1565 TFTFVILSISSWFLIVSWIMAPFIFNPSGFDWLKTVYDFDDFINWLWNAGGVFTKAEQSW 1624

Query: 1566 EAWWLEENSHLRSTGLWGKLLEIILDLRFFFFQYAIVYHLNITGNNTSIAVYFISWVSMI 1625
            EAWWLEENSHLRSTGLWGKLLEIILDLRFFFFQYAIVYHLNITGNNTSIAVYFISWVSMI
Sbjct: 1625 EAWWLEENSHLRSTGLWGKLLEIILDLRFFFFQYAIVYHLNITGNNTSIAVYFISWVSMI 1684

Query: 1626 VLVGIYIVIAYARDKYAAKEHIYYRLVQLIVIVITVLVIVILMEFTPFNTGDLVTCLLAF 1685
             LVGIYIVIAYA+DKYAAKEHIYYRLVQLIVIVITVLVIVILMEFTPFN GDLVTCLLAF
Sbjct: 1685 ALVGIYIVIAYAQDKYAAKEHIYYRLVQLIVIVITVLVIVILMEFTPFNVGDLVTCLLAF 1744

Query: 1686 IPTGWGIISIAQVLRPFLQTTVVWDTVVSMARLYDLLFGVIAMAPLALLSWLPGFQSMQT 1745
            IPTGWGIISIAQVLRPFLQTTVVWDTVVS+ARLYDLLFG+I MAPLALLSWLPGFQSMQT
Sbjct: 1745 IPTGWGIISIAQVLRPFLQTTVVWDTVVSLARLYDLLFGMIVMAPLALLSWLPGFQSMQT 1804

Query: 1746 RILFNEAFSRGLQISRIIAGKKTV 1770
            RILFNEAFSRGLQISRIIAGKKTV
Sbjct: 1805 RILFNEAFSRGLQISRIIAGKKTV 1828

BLAST of HG10021216 vs. ExPASy TrEMBL
Match: A0A0A0LSC7 (1,3-beta-glucan synthase OS=Cucumis sativus OX=3659 GN=Csa_1G073850 PE=3 SV=1)

HSP 1 Score: 3354.7 bits (8697), Expect = 0.0e+00
Identity = 1668/1769 (94.29%), Postives = 1718/1769 (97.12%), Query Frame = 0

Query: 1    MNMRQRPQPAGRGGFPNPPPPVEPYNIIPIHDLLTDHPSLQSPEVRAAAAALRTVGELRR 60
            M MRQRPQ AGRGGFPNP PPVEPYNIIPIHDLLTDHPSLQS EVRAAAAALRTVGELRR
Sbjct: 1    MTMRQRPQTAGRGGFPNPLPPVEPYNIIPIHDLLTDHPSLQSTEVRAAAAALRTVGELRR 60

Query: 61   PSFVPWNPKYDLLDWLGLFFGFQSDNVRNQREHLVLHLANSQMRLRSSPEQADLLDRTVL 120
            PSFVPWNPKYDLLDWLGLFFGFQ+DNVRNQREHLVLHLANSQMRLRSSPE  D+LDRTVL
Sbjct: 61   PSFVPWNPKYDLLDWLGLFFGFQNDNVRNQREHLVLHLANSQMRLRSSPEHPDVLDRTVL 120

Query: 121  RNFRKKLLRSYTLWCSYLGRKSNVRFPRRDQSEERRELLYVSLYLLIWGEAANLRFVPEC 180
            RNFRKKLLRSY+LWCSYLGRKSNVRFP RDQSEERRELLYVSLYLLIWGEAANLRF+PEC
Sbjct: 121  RNFRKKLLRSYSLWCSYLGRKSNVRFPSRDQSEERRELLYVSLYLLIWGEAANLRFLPEC 180

Query: 181  LCYIYHFMAMELNQILDDYIDPETGRPYSPAIHGDCAFLKSVVMPIYQTIKTEVESSRNG 240
            L YIYHFMAMELNQILDDYIDP+TGRPYSPAIHGDCAFLKSVVMPIYQTIK EVESSRNG
Sbjct: 181  LSYIYHFMAMELNQILDDYIDPDTGRPYSPAIHGDCAFLKSVVMPIYQTIKIEVESSRNG 240

Query: 241  TAPHSAWRNYDDINEYFWSRRCFHNLGWPLELSSNFFATTPKNRRVGKTGFVEQRSFWNI 300
            +APHSAWRNYDDINEYFWSRRCF +LGWPL LSSNFFATT KNRRVGKTGFVEQRSFWNI
Sbjct: 241  SAPHSAWRNYDDINEYFWSRRCFRSLGWPLNLSSNFFATTDKNRRVGKTGFVEQRSFWNI 300

Query: 301  FRSFDKIWILLLLFLQASIIVAWQGHQYPWIALKSRDVQVNLLTVFITWSGLRLFQAVLD 360
            FRSFDKIW+LLLLFLQASIIVAWQGHQYPWI LKSRDVQV LLTVFITWSG+RLFQAVLD
Sbjct: 301  FRSFDKIWVLLLLFLQASIIVAWQGHQYPWITLKSRDVQVELLTVFITWSGMRLFQAVLD 360

Query: 361  AGTQYSLVSKETMWLGVRMLLKGLAAIAWIIVFAVFYARIWSQKNSDGFWSDEANGKIFI 420
            AGTQYSLVS+ET+WLGVRMLLK LAA+AWIIVF+VFYARIWSQKNSDGFWSDEA   IF 
Sbjct: 361  AGTQYSLVSRETVWLGVRMLLKCLAAVAWIIVFSVFYARIWSQKNSDGFWSDEATANIFT 420

Query: 421  FLRIVFAFVIPELLALLLFVLPWIRNGLEELDWKVMYLFTWWFHTRIFVGRGLREGLIDN 480
            FLR VFAFVIPELLALL FVLPWIRNGLEELDWKV+YLFTWWFHTRIFVGRGLREGL+DN
Sbjct: 421  FLRAVFAFVIPELLALLFFVLPWIRNGLEELDWKVLYLFTWWFHTRIFVGRGLREGLVDN 480

Query: 481  IKYTIFWVAVLASKFSFSYFFQIQPLVGPTKGLLNLKGPYKWHEFFGSTNIVAVVLLWTP 540
            IKYTIFW+AVLASKFSFSYFFQIQPLVGPTKGLLNLKGPYKWHEFFGSTNIVAVVLLWTP
Sbjct: 481  IKYTIFWIAVLASKFSFSYFFQIQPLVGPTKGLLNLKGPYKWHEFFGSTNIVAVVLLWTP 540

Query: 541  VVLIYLMDLQIWYSIFSSFVGAIVGLFLHLGEIRNIGQLRLRFQFFASAMQFNLMPEEQQ 600
            VVL+YLMDLQIWYSIFSSFVGAIVGLFLHLGEIRNI QLRLRFQFFASAMQFNLMPE Q+
Sbjct: 541  VVLVYLMDLQIWYSIFSSFVGAIVGLFLHLGEIRNIDQLRLRFQFFASAMQFNLMPEVQE 600

Query: 601  LTPKLTRLKKIRDAIHRLKLRYGLGQPYKKIESSMIDTTRFALIWNEILITMREEDLISD 660
            LTPKLTRLKKIRDAIHRLKLRYGLG  YKKIESS IDTT+FALIWNEILITMREEDLISD
Sbjct: 601  LTPKLTRLKKIRDAIHRLKLRYGLGLSYKKIESSRIDTTKFALIWNEILITMREEDLISD 660

Query: 661  RDFDLLELPPNYWNIRVIRWPCFLLCNELLLALSQAIELGNKPDEDLWSKICKNEYQRCA 720
            RDFDLLELPPNYW+IRVIRWPC LLCNELLLALSQA EL + PDE+LW KICKNEYQRCA
Sbjct: 661  RDFDLLELPPNYWSIRVIRWPCVLLCNELLLALSQATELADNPDENLWLKICKNEYQRCA 720

Query: 721  VIEAYDSVKALLLNIVKYGSEENSIVVKVFIDIDNVIGMGKFMEAYNPNVLQEIHTKLIS 780
            VIEAYDSVKALLLNIVKYGSEENSIVVK+FID+DN IG+GKFMEAYNPNVL EIH KLIS
Sbjct: 721  VIEAYDSVKALLLNIVKYGSEENSIVVKIFIDLDNAIGLGKFMEAYNPNVLPEIHAKLIS 780

Query: 781  LVELLIGTKKDLSKAVFILQALYELSIREFPRSKKSTKQLREEGLVPRNPATDEGLLFEN 840
            LVELLIGTKKD+++AVFILQALYELSIREFPRSKKSTKQLREEGLVPRNPATDE  +FEN
Sbjct: 781  LVELLIGTKKDMTQAVFILQALYELSIREFPRSKKSTKQLREEGLVPRNPATDEEFIFEN 840

Query: 841  AVVFPGLEDKYFYRNVQRLHTILTSRDSMHNVPSNLEARRRLAFFSNSLFMNMPRAPYVE 900
            AVVFP +ED++FYRNVQRLHTILTSRDSMHNVPSNLEARRR+AFFSNSLFMNMPRAPYVE
Sbjct: 841  AVVFPSVEDRFFYRNVQRLHTILTSRDSMHNVPSNLEARRRIAFFSNSLFMNMPRAPYVE 900

Query: 901  KMMPFSVLTPYYDEEVVYGKEMLRSENEDGVSTLFYLQRIYEDEWRNFMERMRKKGLEHE 960
            KMMPFSVLTPYYDEEVVYGKEMLRSENEDGVSTLFYLQRIYEDEWRNFMERMRK+GLEHE
Sbjct: 901  KMMPFSVLTPYYDEEVVYGKEMLRSENEDGVSTLFYLQRIYEDEWRNFMERMRKEGLEHE 960

Query: 961  DDIWTKKSRELRLWASYRGQTLSRTVRGMMYYHRALKMFSFLDTASEMDIRMGSQEIASH 1020
            DDIWTKKSR++RLWASYRGQTLSRTVRGMMYYHRAL MFSFLD ASE+DIR GSQEIASH
Sbjct: 961  DDIWTKKSRDVRLWASYRGQTLSRTVRGMMYYHRALNMFSFLDKASEIDIRKGSQEIASH 1020

Query: 1021 GSITRKHALDGLHSTQPPSRNLNRASTGELLYRRSEYGTALMKFTYVVTCQVYGLQKAKR 1080
            GSITRKHALDGL STQPPS +LNRAS GE L+RRS+YG ALMKFTYVVTCQVYGLQKAKR
Sbjct: 1021 GSITRKHALDGLRSTQPPSMDLNRASIGEWLHRRSDYGIALMKFTYVVTCQVYGLQKAKR 1080

Query: 1081 DPRAEEILNLMKDNESLRVAYVDEVHRGRDEVEFYSVLVKYDQELKKEVVIYRIKLPGPL 1140
            DPRAEEILNLMKDNESLRVAYVDEVHRGRDEVEFYSVLVKYDQE  KEVVIYRIKLPGPL
Sbjct: 1081 DPRAEEILNLMKDNESLRVAYVDEVHRGRDEVEFYSVLVKYDQEQGKEVVIYRIKLPGPL 1140

Query: 1141 KIGEGKPENQNHAIIFTRGDAIQTIDMNQDNYFEEALKMRNLLEEFNNSYGIRKPTILGV 1200
            KIGEGKPENQNHAIIFTRGDA+QTIDMNQDNYFEEALKMRNLLEEFN SYGIRKPTILGV
Sbjct: 1141 KIGEGKPENQNHAIIFTRGDALQTIDMNQDNYFEEALKMRNLLEEFNKSYGIRKPTILGV 1200

Query: 1201 RENVFTGSVSSLAWFMSAQETSFVTLAQRVLANPLKVRMHYGHPDVFDRFWFLTRGGISK 1260
            RENVFTGSVSSLAWFMSAQETSFVTLAQRVLANPLKVRMHYGHPDVFDRFWFLTRGGISK
Sbjct: 1201 RENVFTGSVSSLAWFMSAQETSFVTLAQRVLANPLKVRMHYGHPDVFDRFWFLTRGGISK 1260

Query: 1261 ASKVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGFNQISMFEAKVASGNGEQVLS 1320
            ASKVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGFNQISMFEAKVASGNGEQVLS
Sbjct: 1261 ASKVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGFNQISMFEAKVASGNGEQVLS 1320

Query: 1321 RDIYRLGHRLDFFRVLSVFYTTVGYYFNTMLVVLSVYTFLWGRLYLALSGVEDAAVASSS 1380
            RDIYRLGHRLDFFRVLSVFYTTVGYYFNTMLVVLSVY+FLWGRLYLALSGVEDAA+ASS+
Sbjct: 1321 RDIYRLGHRLDFFRVLSVFYTTVGYYFNTMLVVLSVYSFLWGRLYLALSGVEDAAIASST 1380

Query: 1381 GNNRALGAILNQQFIIQLGLFTALPMIVENSLEHGFLPAIWNFLTMQLQLASFFYTFSLG 1440
            GNNRALGAILNQQFIIQLGLFTALPMIVENSLEHGFLPA+WNFLTMQLQLASFFYTFSLG
Sbjct: 1381 GNNRALGAILNQQFIIQLGLFTALPMIVENSLEHGFLPAVWNFLTMQLQLASFFYTFSLG 1440

Query: 1441 TRTHFFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFVKAIELGVILIVYASRS 1500
            TRTHFFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFVKAIELGVILIVYASRS
Sbjct: 1441 TRTHFFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFVKAIELGVILIVYASRS 1500

Query: 1501 PLARSTFTFVILSISSWFLIVSWIMAPFIFNPSGFDWLKTVYDFDDFINWLWNAGGVFTK 1560
            PLA +TFTFVILSISSWFLIVSWIMAPFIFNPSGFDWLKTVYDFDDFI+WLWNAGGVFTK
Sbjct: 1501 PLATNTFTFVILSISSWFLIVSWIMAPFIFNPSGFDWLKTVYDFDDFISWLWNAGGVFTK 1560

Query: 1561 AEQSWEAWWLEENSHLRSTGLWGKLLEIILDLRFFFFQYAIVYHLNITGNNTSIAVYFIS 1620
            AEQSWEAWWLEENSHLRSTGLWGKLLEIILDLRFFFFQYAIVYHLNITGNNTSIAVYFIS
Sbjct: 1561 AEQSWEAWWLEENSHLRSTGLWGKLLEIILDLRFFFFQYAIVYHLNITGNNTSIAVYFIS 1620

Query: 1621 WVSMIVLVGIYIVIAYARDKYAAKEHIYYRLVQLIVIVITVLVIVILMEFTPFNTGDLVT 1680
            WVSMI LVGIYIV+AYARDKYAAKEHIYYRLVQLIVIVITVLVIVILMEFTPFN GDLVT
Sbjct: 1621 WVSMIALVGIYIVVAYARDKYAAKEHIYYRLVQLIVIVITVLVIVILMEFTPFNVGDLVT 1680

Query: 1681 CLLAFIPTGWGIISIAQVLRPFLQTTVVWDTVVSMARLYDLLFGVIAMAPLALLSWLPGF 1740
            CLLAFIPTGWGIISIAQVLRPFLQTTVVWDTVVS+ARLYDLLFG+IAMAPLALLSWLPGF
Sbjct: 1681 CLLAFIPTGWGIISIAQVLRPFLQTTVVWDTVVSLARLYDLLFGMIAMAPLALLSWLPGF 1740

Query: 1741 QSMQTRILFNEAFSRGLQISRIIAGKKTV 1770
            QSMQTRILFNEAFSRGLQISRIIAGKKTV
Sbjct: 1741 QSMQTRILFNEAFSRGLQISRIIAGKKTV 1769

BLAST of HG10021216 vs. ExPASy TrEMBL
Match: A0A6J1KR86 (1,3-beta-glucan synthase OS=Cucurbita maxima OX=3661 GN=LOC111495751 PE=3 SV=1)

HSP 1 Score: 3342.4 bits (8665), Expect = 0.0e+00
Identity = 1661/1770 (93.84%), Postives = 1721/1770 (97.23%), Query Frame = 0

Query: 1    MNMRQRPQPAGRGGFPN-PPPPVEPYNIIPIHDLLTDHPSLQSPEVRAAAAALRTVGELR 60
            MN+RQRPQP GRGGF N PPPPVEPYNIIPIHDLLTDHPSLQSPEVRAAAAALRTVGELR
Sbjct: 1    MNLRQRPQPVGRGGFQNAPPPPVEPYNIIPIHDLLTDHPSLQSPEVRAAAAALRTVGELR 60

Query: 61   RPSFVPWNPKYDLLDWLGLFFGFQSDNVRNQREHLVLHLANSQMRLRSSPEQADLLDRTV 120
            RPSFVPWNPKYDLLDWLGLFFGFQ DNVRNQREHLVLHLANSQMRLRSSPE+ D LDRTV
Sbjct: 61   RPSFVPWNPKYDLLDWLGLFFGFQDDNVRNQREHLVLHLANSQMRLRSSPERPDELDRTV 120

Query: 121  LRNFRKKLLRSYTLWCSYLGRKSNVRFPRRDQSEERRELLYVSLYLLIWGEAANLRFVPE 180
            LRNFRKKLLR+YTLWCSYLGRKSNVR   RD+SE RRELLYVSLYLLIWGEAANLRF PE
Sbjct: 121  LRNFRKKLLRNYTLWCSYLGRKSNVRLSSRDKSEGRRELLYVSLYLLIWGEAANLRFAPE 180

Query: 181  CLCYIYHFMAMELNQILDDYIDPETGRPYSPAIHGDCAFLKSVVMPIYQTIKTEVESSRN 240
            CLCYIYHFMAMELNQILDDYIDP+TGRPYSPAIHGDCAFLKSVVMPIY+TIKTEVESSRN
Sbjct: 181  CLCYIYHFMAMELNQILDDYIDPDTGRPYSPAIHGDCAFLKSVVMPIYKTIKTEVESSRN 240

Query: 241  GTAPHSAWRNYDDINEYFWSRRCFHNLGWPLELSSNFFATTPKNRRVGKTGFVEQRSFWN 300
            G+APHSAWRNYDDINEYFWSRRCF +LGWPL LSSNFFATTPKNRRVGKTG+VEQRSFWN
Sbjct: 241  GSAPHSAWRNYDDINEYFWSRRCFRSLGWPLNLSSNFFATTPKNRRVGKTGYVEQRSFWN 300

Query: 301  IFRSFDKIWILLLLFLQASIIVAWQGHQYPWIALKSRDVQVNLLTVFITWSGLRLFQAVL 360
            IFRSFDKIWILLLLFLQASIIVAWQG QYPWIALKSRDVQV LLTVFITWSGLRLFQAVL
Sbjct: 301  IFRSFDKIWILLLLFLQASIIVAWQGQQYPWIALKSRDVQVELLTVFITWSGLRLFQAVL 360

Query: 361  DAGTQYSLVSKETMWLGVRMLLKGLAAIAWIIVFAVFYARIWSQKNSDGFWSDEANGKIF 420
            DAGTQYSLVS+ETMWLGVRMLLKGLAAIAWIIVF++FYARIWS+K SDGFWSDEANG+IF
Sbjct: 361  DAGTQYSLVSRETMWLGVRMLLKGLAAIAWIIVFSIFYARIWSRKKSDGFWSDEANGEIF 420

Query: 421  IFLRIVFAFVIPELLALLLFVLPWIRNGLEELDWKVMYLFTWWFHTRIFVGRGLREGLID 480
            IFLR VFAF+IPE+LALLLFVLPWIRNGLEELDWKV+Y+FTWWFHTR+F+GRGLREGLID
Sbjct: 421  IFLRAVFAFLIPEMLALLLFVLPWIRNGLEELDWKVLYIFTWWFHTRMFIGRGLREGLID 480

Query: 481  NIKYTIFWVAVLASKFSFSYFFQIQPLVGPTKGLLNLKGPYKWHEFFGSTNIVAVVLLWT 540
            NIKY+IFWVAVLA+KF+FSYFFQIQPLVGPTKGLLNLKGPYKWHEFFGSTN+VAVVLLWT
Sbjct: 481  NIKYSIFWVAVLAAKFAFSYFFQIQPLVGPTKGLLNLKGPYKWHEFFGSTNVVAVVLLWT 540

Query: 541  PVVLIYLMDLQIWYSIFSSFVGAIVGLFLHLGEIRNIGQLRLRFQFFASAMQFNLMPEEQ 600
            PVVLIYLMDLQIWY+IFSS VGA VGLFLHLGEIRNIGQL LRFQFFASAMQF+LMPE Q
Sbjct: 541  PVVLIYLMDLQIWYTIFSSLVGATVGLFLHLGEIRNIGQLSLRFQFFASAMQFSLMPEVQ 600

Query: 601  QLTPKLTRLKKIRDAIHRLKLRYGLGQPYKKIESSMIDTTRFALIWNEILITMREEDLIS 660
            QLTPK+TRLKKIRDAIHRLKLRYGLGQPYKKIESS IDTT+FALIWNEI+ITMREEDLIS
Sbjct: 601  QLTPKMTRLKKIRDAIHRLKLRYGLGQPYKKIESSRIDTTKFALIWNEIIITMREEDLIS 660

Query: 661  DRDFDLLELPPNYWNIRVIRWPCFLLCNELLLALSQAIELGNKPDEDLWSKICKNEYQRC 720
            DRDFDLLELPPN WNIRVIRWPCFLLCNELLLALSQA EL NKPDEDLWSKICKNEYQRC
Sbjct: 661  DRDFDLLELPPNCWNIRVIRWPCFLLCNELLLALSQATELANKPDEDLWSKICKNEYQRC 720

Query: 721  AVIEAYDSVKALLLNIVKYGSEENSIVVKVFIDIDNVIGMGKFMEAYNPNVLQEIHTKLI 780
            AVIEAYDSVKA+LL IVKYGSEENSIV K+F+DIDNVIG+GKF++AYN NVL EIHTKLI
Sbjct: 721  AVIEAYDSVKAVLLTIVKYGSEENSIVAKLFMDIDNVIGLGKFLDAYNSNVLPEIHTKLI 780

Query: 781  SLVELLIGTKKDLSKAVFILQALYELSIREFPRSKKSTKQLREEGLVPRNPATDEGLLFE 840
            SL++LLIGTKKDLSKAV ILQALYELSIREFPR KKSTKQLREEGLVPRNPATDEGLLF 
Sbjct: 781  SLLDLLIGTKKDLSKAVNILQALYELSIREFPRLKKSTKQLREEGLVPRNPATDEGLLFV 840

Query: 841  NAVVFPGLEDKYFYRNVQRLHTILTSRDSMHNVPSNLEARRRLAFFSNSLFMNMPRAPYV 900
             AV FP LEDK FYRNVQRLHTILTSRDSMH VPSNLEARRRLAFFSNSLFMN+PRAPYV
Sbjct: 841  KAVEFPDLEDKMFYRNVQRLHTILTSRDSMHGVPSNLEARRRLAFFSNSLFMNIPRAPYV 900

Query: 901  EKMMPFSVLTPYYDEEVVYGKEMLRSENEDGVSTLFYLQRIYEDEWRNFMERMRKKGLEH 960
            EKMMPFSVLTPYYDEEVVYGKEMLRSENEDGVSTLFYLQRIYEDEWRNFMERMRK+GLEH
Sbjct: 901  EKMMPFSVLTPYYDEEVVYGKEMLRSENEDGVSTLFYLQRIYEDEWRNFMERMRKEGLEH 960

Query: 961  EDDIWTKKSRELRLWASYRGQTLSRTVRGMMYYHRALKMFSFLDTASEMDIRMGSQEIAS 1020
            EDDIWTKKSR+LRLWASYRGQTLSRTVRGMMYYHRALKMFSFLDTASE+DIRMGSQEIAS
Sbjct: 961  EDDIWTKKSRDLRLWASYRGQTLSRTVRGMMYYHRALKMFSFLDTASEVDIRMGSQEIAS 1020

Query: 1021 HGSITRKHALDGLHSTQPPSRNLNRASTGELLYRRSEYGTALMKFTYVVTCQVYGLQKAK 1080
            HGSITRKHALDGL STQPP RNLNR+++GELL+R  EYGTALMKFTYVVTCQVYGLQKAK
Sbjct: 1021 HGSITRKHALDGLRSTQPPFRNLNRSTSGELLFRGYEYGTALMKFTYVVTCQVYGLQKAK 1080

Query: 1081 RDPRAEEILNLMKDNESLRVAYVDEVHRGRDEVEFYSVLVKYDQELKKEVVIYRIKLPGP 1140
            RD RAEEILNLMK+NE+LRVAYVDEVHRGRDEVEFYSVLVKYDQEL+KEVVIYRI+LPGP
Sbjct: 1081 RDSRAEEILNLMKENEALRVAYVDEVHRGRDEVEFYSVLVKYDQELQKEVVIYRIRLPGP 1140

Query: 1141 LKIGEGKPENQNHAIIFTRGDAIQTIDMNQDNYFEEALKMRNLLEEFNNSYGIRKPTILG 1200
            LKIGEGKPENQNHAIIFTRGDAIQTIDMNQ+NYFEEALKMRNLLEEF NSYGIRKPTILG
Sbjct: 1141 LKIGEGKPENQNHAIIFTRGDAIQTIDMNQENYFEEALKMRNLLEEFKNSYGIRKPTILG 1200

Query: 1201 VRENVFTGSVSSLAWFMSAQETSFVTLAQRVLANPLKVRMHYGHPDVFDRFWFLTRGGIS 1260
            VRENVFTGSVSSLAWFMSAQETSFVTLAQRVLANPLKVRMHYGHPDVFDRFWFLTRGGIS
Sbjct: 1201 VRENVFTGSVSSLAWFMSAQETSFVTLAQRVLANPLKVRMHYGHPDVFDRFWFLTRGGIS 1260

Query: 1261 KASKVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGFNQISMFEAKVASGNGEQVL 1320
            KAS+VINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGFNQISMFEAKVASGNGEQVL
Sbjct: 1261 KASRVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGFNQISMFEAKVASGNGEQVL 1320

Query: 1321 SRDIYRLGHRLDFFRVLSVFYTTVGYYFNTMLVVLSVYTFLWGRLYLALSGVEDAAVASS 1380
            SRD+YRLGHRLDFFR+LSVFYTTVGYYFNTMLVVLSV+ FLWGRLYLALSGVEDAAVASS
Sbjct: 1321 SRDVYRLGHRLDFFRMLSVFYTTVGYYFNTMLVVLSVFVFLWGRLYLALSGVEDAAVASS 1380

Query: 1381 SGNNRALGAILNQQFIIQLGLFTALPMIVENSLEHGFLPAIWNFLTMQLQLASFFYTFSL 1440
            SGNNRALGAILNQQFIIQLGLFTALPMIVENSLEHGFLPAIWNFLTMQLQLASFFYTFSL
Sbjct: 1381 SGNNRALGAILNQQFIIQLGLFTALPMIVENSLEHGFLPAIWNFLTMQLQLASFFYTFSL 1440

Query: 1441 GTRTHFFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFVKAIELGVILIVYASR 1500
            GTRTHFFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFVKAIELGVILIVYASR
Sbjct: 1441 GTRTHFFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFVKAIELGVILIVYASR 1500

Query: 1501 SPLARSTFTFVILSISSWFLIVSWIMAPFIFNPSGFDWLKTVYDFDDFINWLWNAGGVFT 1560
            SPLA+STFTFVILSISSWFLIVSWIMAPFIFNPSGFDWLKTVYDFDDFINWLWN GGVFT
Sbjct: 1501 SPLAKSTFTFVILSISSWFLIVSWIMAPFIFNPSGFDWLKTVYDFDDFINWLWNTGGVFT 1560

Query: 1561 KAEQSWEAWWLEENSHLRSTGLWGKLLEIILDLRFFFFQYAIVYHLNITGNNTSIAVYFI 1620
            KAEQSWEAWWLEENSHLR+TGLWGKLLEIILDLRFFFFQYAIVYHLNITGNNTSIAVYFI
Sbjct: 1561 KAEQSWEAWWLEENSHLRTTGLWGKLLEIILDLRFFFFQYAIVYHLNITGNNTSIAVYFI 1620

Query: 1621 SWVSMIVLVGIYIVIAYARDKYAAKEHIYYRLVQLIVIVITVLVIVILMEFTPFNTGDLV 1680
            SWVSMIVLVGIYIVIAYA+DKYAAKEHIYYRLVQLIVIV+ VLVIVILMEFTPFN GDLV
Sbjct: 1621 SWVSMIVLVGIYIVIAYAQDKYAAKEHIYYRLVQLIVIVVIVLVIVILMEFTPFNMGDLV 1680

Query: 1681 TCLLAFIPTGWGIISIAQVLRPFLQTTVVWDTVVSMARLYDLLFGVIAMAPLALLSWLPG 1740
            TCLLAFIPTGWGIISIAQVLRPFLQTTVVWDTVVS ARLYDLLFG+I MAPLALLSWLPG
Sbjct: 1681 TCLLAFIPTGWGIISIAQVLRPFLQTTVVWDTVVSSARLYDLLFGMIVMAPLALLSWLPG 1740

Query: 1741 FQSMQTRILFNEAFSRGLQISRIIAGKKTV 1770
            FQSMQTRILFNEAFSRGLQISRIIAGKKT+
Sbjct: 1741 FQSMQTRILFNEAFSRGLQISRIIAGKKTI 1770

BLAST of HG10021216 vs. ExPASy TrEMBL
Match: A0A6J1EN13 (1,3-beta-glucan synthase OS=Cucurbita moschata OX=3662 GN=LOC111434073 PE=3 SV=1)

HSP 1 Score: 3324.6 bits (8619), Expect = 0.0e+00
Identity = 1652/1777 (92.97%), Postives = 1716/1777 (96.57%), Query Frame = 0

Query: 1    MNMRQRPQPAGRGGFPNPPPPVEPYNIIPIHDLLTDHPSLQSPEVRAAAAALRTVGELRR 60
            MN+RQRPQPAGRGGF N PPPVEP+NIIPIHDLLTDHPSLQSPEVRAAAAALRTVGELRR
Sbjct: 1    MNLRQRPQPAGRGGFQNAPPPVEPFNIIPIHDLLTDHPSLQSPEVRAAAAALRTVGELRR 60

Query: 61   PSFVPWNPKYDLLDWLGLFFGFQSDNVRNQREHLVLHLANSQMRLRSSPEQADLLDRTVL 120
            PSFVPWNPKYDLLDWLGLFFGFQ DNVRNQREHLVLHLANSQMRLRSSPE+ D LDRTVL
Sbjct: 61   PSFVPWNPKYDLLDWLGLFFGFQDDNVRNQREHLVLHLANSQMRLRSSPERPDELDRTVL 120

Query: 121  RNFRKKLLRSYTLWCSYLGRKSNVRFPRRDQSEERRELLYVSLYLLIWGEAANLRFVPEC 180
            RNFRKKLLR+YTLWCSYLGRKSNVR   RD+SE RRELLYVSLYLLIWGEAANLRF PEC
Sbjct: 121  RNFRKKLLRNYTLWCSYLGRKSNVRLSSRDKSEGRRELLYVSLYLLIWGEAANLRFAPEC 180

Query: 181  LCYIYHFMAMELNQILDDYIDPETGRPYSPAIHGDCAFLKSVVMPIYQTIKTEVESSRNG 240
            LCYIYHFMAMELNQILDDYIDP+TGRPYSPAIHGDCAFL+SVVMPIY+TIKTEVESSRNG
Sbjct: 181  LCYIYHFMAMELNQILDDYIDPDTGRPYSPAIHGDCAFLRSVVMPIYKTIKTEVESSRNG 240

Query: 241  TAPHSAWRNYDDINEYFWSRRCFHNLGWPLELSSNFFATTPKNRRVGKTGFVEQRSFWNI 300
            +APHSAWRNYDDINEYFWSRRCF +LGWPL LSSNFFATTPKNRRVGKTG+VEQRSFWNI
Sbjct: 241  SAPHSAWRNYDDINEYFWSRRCFRSLGWPLNLSSNFFATTPKNRRVGKTGYVEQRSFWNI 300

Query: 301  FRSFDKIWILLLLFLQASIIVAWQGHQYPWIALKSRDVQVNLLTVFITWSGLRLFQAVLD 360
            FRSFDKIW+LLLLFLQASIIVAWQG QYPWIALKSRDVQV LLTVFITWSGLRLFQAVLD
Sbjct: 301  FRSFDKIWLLLLLFLQASIIVAWQGQQYPWIALKSRDVQVELLTVFITWSGLRLFQAVLD 360

Query: 361  AGTQYSLVSKETMWLGVRMLLKGLAAIAWIIVFAVFYARIWSQKNSDGFWSDEANGKIFI 420
            AGTQYSLVS+ETMWLGVRMLLKGLAAIAWIIVF++FYARIWSQK SDGFWSDEANG+IF+
Sbjct: 361  AGTQYSLVSRETMWLGVRMLLKGLAAIAWIIVFSIFYARIWSQKKSDGFWSDEANGEIFL 420

Query: 421  FLRIVFAFVIPELLALLLFVLPWIRNGLEELDWKVMYLFTWWFHTRIFVGRGLREGLIDN 480
            FLR VFAF+IPE+LALLLFVLPWIRNGLEELDWKV+YLFTWWFHTR+F+GRGLREGLIDN
Sbjct: 421  FLRAVFAFLIPEMLALLLFVLPWIRNGLEELDWKVLYLFTWWFHTRMFIGRGLREGLIDN 480

Query: 481  IKYTIFWVAVLASKFSFSYFFQIQPLVGPTKGLLNLKGPYKWHEFFGSTNIVAVVLLWTP 540
            IKY+IFWVAVLA+KFSFSYFFQIQPLVGPTKGLLNLKGPYKWHEFFGSTN+VAVVLLWTP
Sbjct: 481  IKYSIFWVAVLAAKFSFSYFFQIQPLVGPTKGLLNLKGPYKWHEFFGSTNVVAVVLLWTP 540

Query: 541  VVLIYLMDLQIWYSIFSSFVGAIVGLFLHLGEIRNIGQLRLRFQFFASAMQFNLMPEEQQ 600
            VVLIYLMDLQIWYSIFSS VGA VGLFLHLGEIRNIGQL LRFQFFASAMQF+LMPE QQ
Sbjct: 541  VVLIYLMDLQIWYSIFSSLVGATVGLFLHLGEIRNIGQLSLRFQFFASAMQFSLMPEVQQ 600

Query: 601  LTPKLTRLKKIRDAIHRLKLRYGLGQPYKKIESSMIDTTRFALIWNEILITMREEDLISD 660
            LTPK+TRLKKIRDAIHRLKLRYGLGQPYKKIESS IDTT+FALIWNEI+ITMREEDLISD
Sbjct: 601  LTPKMTRLKKIRDAIHRLKLRYGLGQPYKKIESSRIDTTKFALIWNEIIITMREEDLISD 660

Query: 661  RDFDLLELPPNYWNIRVIRWPCFLLCNELLLALSQAIELGNKPDEDLWSKICKNEYQRCA 720
            RDFDLLELPPN WNIRVIRWPCFLLCNELLLALSQA EL + PDEDLWSKICKNEYQRCA
Sbjct: 661  RDFDLLELPPNCWNIRVIRWPCFLLCNELLLALSQATELADNPDEDLWSKICKNEYQRCA 720

Query: 721  VIEAYDSVKALLLNIVKYGSEENSIVVKVFIDIDNVIGMGKFMEAYNPNVLQEIHTKLIS 780
            VIEAYDSVKA+LL IVKYGSEENSIV K+F+DIDNVIG+GKF++AYN NVL EIHTKLIS
Sbjct: 721  VIEAYDSVKAVLLTIVKYGSEENSIVAKLFMDIDNVIGLGKFLDAYNSNVLPEIHTKLIS 780

Query: 781  LVELLIGTKKDL--------SKAVFILQALYELSIREFPRSKKSTKQLREEGLVPRNPAT 840
            L+++LI T+KD+        SKAV ILQALYELSIREFP+ KKSTKQLREEGLVPRNP T
Sbjct: 781  LLDILIETEKDVSKAVNILQSKAVNILQALYELSIREFPKLKKSTKQLREEGLVPRNPTT 840

Query: 841  DEGLLFENAVVFPGLEDKYFYRNVQRLHTILTSRDSMHNVPSNLEARRRLAFFSNSLFMN 900
            DEGLLF  AV FP LEDK FYRNVQRLHTILTSRDSMH VPSNLEARRRLAFFSNSLFMN
Sbjct: 841  DEGLLFVKAVEFPNLEDKMFYRNVQRLHTILTSRDSMHGVPSNLEARRRLAFFSNSLFMN 900

Query: 901  MPRAPYVEKMMPFSVLTPYYDEEVVYGKEMLRSENEDGVSTLFYLQRIYEDEWRNFMERM 960
            +PRAPYVEKMMPFSVLTPYYDEEVVYGKEMLRSENEDGVSTLFYLQRIYEDEWRNFMERM
Sbjct: 901  IPRAPYVEKMMPFSVLTPYYDEEVVYGKEMLRSENEDGVSTLFYLQRIYEDEWRNFMERM 960

Query: 961  RKKGLEHEDDIWTKKSRELRLWASYRGQTLSRTVRGMMYYHRALKMFSFLDTASEMDIRM 1020
            RK+GLEHEDDIWTKKSR+LRLWASYRGQTLSRTVRGMMYYHRALKMFSFLDTASEMDIRM
Sbjct: 961  RKEGLEHEDDIWTKKSRDLRLWASYRGQTLSRTVRGMMYYHRALKMFSFLDTASEMDIRM 1020

Query: 1021 GSQEIASHGSITRKHALDGLHSTQPPSRNLNRASTGELLYRRSEYGTALMKFTYVVTCQV 1080
            GSQEIASHGSITRKHALDGLHSTQPP RNL R+++GELL+R  EYGTALMKFTYVVTCQV
Sbjct: 1021 GSQEIASHGSITRKHALDGLHSTQPPFRNLTRSTSGELLFRGYEYGTALMKFTYVVTCQV 1080

Query: 1081 YGLQKAKRDPRAEEILNLMKDNESLRVAYVDEVHRGRDEVEFYSVLVKYDQELKKEVVIY 1140
            YGLQK KRDPRAEEILNLMK+NE+LRVAYVDEVH GRDEVEFYSVLVKYDQEL+KEVVIY
Sbjct: 1081 YGLQKTKRDPRAEEILNLMKENEALRVAYVDEVHHGRDEVEFYSVLVKYDQELQKEVVIY 1140

Query: 1141 RIKLPGPLKIGEGKPENQNHAIIFTRGDAIQTIDMNQDNYFEEALKMRNLLEEFNNSYGI 1200
            RI+LPGPLKIGEGKPENQNHAIIFTRGDAIQTIDMNQ+NYFEEALKMRNLLEEF NSYGI
Sbjct: 1141 RIRLPGPLKIGEGKPENQNHAIIFTRGDAIQTIDMNQENYFEEALKMRNLLEEFKNSYGI 1200

Query: 1201 RKPTILGVRENVFTGSVSSLAWFMSAQETSFVTLAQRVLANPLKVRMHYGHPDVFDRFWF 1260
            RKPTILGVRENVFTGSVSSLAWFMSAQETSFVTLAQRVLANPLKVRMHYGHPDVFDRFWF
Sbjct: 1201 RKPTILGVRENVFTGSVSSLAWFMSAQETSFVTLAQRVLANPLKVRMHYGHPDVFDRFWF 1260

Query: 1261 LTRGGISKASKVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGFNQISMFEAKVAS 1320
            LTRGGISKAS+VINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGFNQISMFEAKVAS
Sbjct: 1261 LTRGGISKASRVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGFNQISMFEAKVAS 1320

Query: 1321 GNGEQVLSRDIYRLGHRLDFFRVLSVFYTTVGYYFNTMLVVLSVYTFLWGRLYLALSGVE 1380
            GNGEQVLSRD+YRLGHRLDFFR+LSVFYTTVGYYFNTMLVVLSV+ FLWGRLYLALSGVE
Sbjct: 1321 GNGEQVLSRDVYRLGHRLDFFRMLSVFYTTVGYYFNTMLVVLSVFVFLWGRLYLALSGVE 1380

Query: 1381 DAAVASSSGNNRALGAILNQQFIIQLGLFTALPMIVENSLEHGFLPAIWNFLTMQLQLAS 1440
            DAAVASSSGNNRALGAILNQQFIIQLGLFTALPMIVENSLEHGFLPAIWNFLTMQLQL+S
Sbjct: 1381 DAAVASSSGNNRALGAILNQQFIIQLGLFTALPMIVENSLEHGFLPAIWNFLTMQLQLSS 1440

Query: 1441 FFYTFSLGTRTHFFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFVKAIELGVI 1500
            FFYTFSLGTRTHFFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFVKAIELGVI
Sbjct: 1441 FFYTFSLGTRTHFFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFVKAIELGVI 1500

Query: 1501 LIVYASRSPLARSTFTFVILSISSWFLIVSWIMAPFIFNPSGFDWLKTVYDFDDFINWLW 1560
            LIVYASRSPLA+STFTFVILSISSWFLIVSWIMAPFIFNPSGFDWLKTVYDFDDFINWLW
Sbjct: 1501 LIVYASRSPLAKSTFTFVILSISSWFLIVSWIMAPFIFNPSGFDWLKTVYDFDDFINWLW 1560

Query: 1561 NAGGVFTKAEQSWEAWWLEENSHLRSTGLWGKLLEIILDLRFFFFQYAIVYHLNITGNNT 1620
            N GGVFTKAEQSWEAWWLEENSHLR+TGLWGKLLEIILDLRFFFFQYAIVYHLNITGN T
Sbjct: 1561 NTGGVFTKAEQSWEAWWLEENSHLRTTGLWGKLLEIILDLRFFFFQYAIVYHLNITGNKT 1620

Query: 1621 SIAVYFISWVSMIVLVGIYIVIAYARDKYAAKEHIYYRLVQLIVIVITVLVIVILMEFTP 1680
            SIAVYFISWVSMIVLVGIYIVIAYA+DKYAAKEHIYYRLVQLIVIV+ VLVIVILMEFTP
Sbjct: 1621 SIAVYFISWVSMIVLVGIYIVIAYAQDKYAAKEHIYYRLVQLIVIVVIVLVIVILMEFTP 1680

Query: 1681 FNTGDLVTCLLAFIPTGWGIISIAQVLRPFLQTTVVWDTVVSMARLYDLLFGVIAMAPLA 1740
            FNT DLVTCLLAFIPTGWGIISIAQVLRPFLQTTVVWDTVVS ARLYDLLFG+I MAPLA
Sbjct: 1681 FNTADLVTCLLAFIPTGWGIISIAQVLRPFLQTTVVWDTVVSSARLYDLLFGMIVMAPLA 1740

Query: 1741 LLSWLPGFQSMQTRILFNEAFSRGLQISRIIAGKKTV 1770
            LLSWLPGFQSMQTRILFNEAFSRGLQISRIIAGKKTV
Sbjct: 1741 LLSWLPGFQSMQTRILFNEAFSRGLQISRIIAGKKTV 1777

BLAST of HG10021216 vs. TAIR 10
Match: AT4G04970.1 (glucan synthase-like 1 )

HSP 1 Score: 2533.8 bits (6566), Expect = 0.0e+00
Identity = 1259/1774 (70.97%), Postives = 1487/1774 (83.82%), Query Frame = 0

Query: 4    RQRPQPAGRGGFPNPPPPVEPYNIIPIHDLLTDHPSLQSPEVRAAAAALRTVGELRRPSF 63
            RQRP  A         P +E YNIIPIHD LT+HPSL+ PEVRAAAAALR VG+L +P F
Sbjct: 3    RQRPSVA----TARDAPSLEVYNIIPIHDFLTEHPSLRYPEVRAAAAALRIVGDLPKPPF 62

Query: 64   VPWNPKYDLLDWLGLFFGFQSDNVRNQREHLVLHLANSQMRLRSSPEQADLLDRTVLRNF 123
              + P+ DL+DWLGL FGFQ DNVRNQRE+LVLHLANSQMRL+  P   D LD TVLR F
Sbjct: 63   ADFTPRMDLMDWLGLLFGFQIDNVRNQRENLVLHLANSQMRLQPPPRHPDGLDPTVLRRF 122

Query: 124  RKKLLRSYTLWCSYLGRKSNVRFPRRDQSEE------RRELLYVSLYLLIWGEAANLRFV 183
            RKKLLR+YT WCS+LG + +V  P + + +       RRELLYV+LYLLIWGE+ANLRF+
Sbjct: 123  RKKLLRNYTNWCSFLGVRCHVTSPIQSRHQTNAVLNLRRELLYVALYLLIWGESANLRFM 182

Query: 184  PECLCYIYHFMAMELNQILDDYIDPETGRPYSPAIHGDCAFLKSVVMPIYQTIKTEVESS 243
            PECLCYI+H MAMELN++L    D  TG PY P+  GDCAFLKSVVMPIY+T+KTEVESS
Sbjct: 183  PECLCYIFHHMAMELNKVLAGEFDDMTGMPYWPSFSGDCAFLKSVVMPIYKTVKTEVESS 242

Query: 244  RNGTAPHSAWRNYDDINEYFWSRRCFHNLGWPLELSSNFFATTPKNRRVGKTGFVEQRSF 303
             NGT PHSAWRNYDDINEYFWS+R   +L WPL+ +SNFF TTPK+ RVGKTGFVEQRSF
Sbjct: 243  NNGTKPHSAWRNYDDINEYFWSKRALKSLKWPLDYTSNFFDTTPKSSRVGKTGFVEQRSF 302

Query: 304  WNIFRSFDKIWILLLLFLQASIIVAWQGHQYPWIALKSRDVQVNLLTVFITWSGLRLFQA 363
            WN++RSFD++WILLLL+LQA+IIVA    ++PW   + RDV+V LLTVFI+W+GLRL Q+
Sbjct: 303  WNVYRSFDRLWILLLLYLQAAIIVATSDVKFPW---QDRDVEVALLTVFISWAGLRLLQS 362

Query: 364  VLDAGTQYSLVSKETMWLGVRMLLKGLAAIAWIIVFAVFYARIWSQKNSDGFWSDEANGK 423
            VLDA TQYSLVS+ET WL +R+ LK + A+AW ++F+VFYARIWSQKN DG WS  AN +
Sbjct: 363  VLDASTQYSLVSRETYWLFIRLTLKFVVAVAWTVLFSVFYARIWSQKNKDGVWSRAANER 422

Query: 424  IFIFLRIVFAFVIPELLALLLFVLPWIRNGLEELDWKVMYLFTWWFHTRIFVGRGLREGL 483
            +  FL++VF +VIPELLAL+LF++P IRN +EEL+  V+Y  TWWF+++ FVGRG+REGL
Sbjct: 423  VVTFLKVVFVYVIPELLALVLFIVPCIRNWVEELNLGVVYFLTWWFYSKTFVGRGMREGL 482

Query: 484  IDNIKYTIFWVAVLASKFSFSYFFQIQPLVGPTKGLLNLK-GPYKWHEFFGSTNIVAVVL 543
            +DN+KYT+FW+ VLA+KF FSYF QI+PL+ PT+ LLNLK   Y WHEFFGST+ +AV +
Sbjct: 483  VDNVKYTLFWIIVLATKFIFSYFLQIRPLIAPTRALLNLKDATYNWHEFFGSTHRIAVGM 542

Query: 544  LWTPVVLIYLMDLQIWYSIFSSFVGAIVGLFLHLGEIRNIGQLRLRFQFFASAMQFNLMP 603
            LW PV+L+YLMDLQIWYSI+SS VGA +GLF HLGEIRNI QLRLRFQFF+SAMQFNL P
Sbjct: 543  LWLPVILVYLMDLQIWYSIYSSLVGATIGLFSHLGEIRNIDQLRLRFQFFSSAMQFNLKP 602

Query: 604  EEQQLTPKLTRLKKIRDAIHRLKLRYGLGQPYKKIESSMIDTTRFALIWNEILITMREED 663
            EE  L+PK T LKK RDAIHRLKLRYG+GQP+ KIESS ++ T FALIWNEI++T REED
Sbjct: 603  EEHLLSPKATMLKKARDAIHRLKLRYGIGQPFNKIESSQVEATWFALIWNEIILTFREED 662

Query: 664  LISDRDFDLLELPPNYWNIRVIRWPCFLLCNELLLALSQAIELGNKPDEDLWSKICKNEY 723
            LISDR+ +LLELPPN WNIRVIRWPCFLLCNELLLALSQA EL + PD  LWSKIC +EY
Sbjct: 663  LISDREVELLELPPNCWNIRVIRWPCFLLCNELLLALSQANELCDAPDHWLWSKICSSEY 722

Query: 724  QRCAVIEAYDSVKALLLNIVKYGSEENSIVVKVFIDIDNVIGMGKFMEAYNPNVLQEIHT 783
            +RCAV+EA+DS+K ++L IVK G+EE SI+ ++F++ID  +   K  E Y   VL  IH 
Sbjct: 723  RRCAVMEAFDSIKFVILKIVKNGTEEESILNRLFMEIDENVENEKITEVYKLTVLLRIHE 782

Query: 784  KLISLVELLIGTKKDLSKAVFILQALYELSIREFPRSKKSTKQLREEGLVPRNPATDEGL 843
            KLISL+E L+  +K + + V ILQALYEL   EFP++++ST QLR+ GL P +   D  L
Sbjct: 783  KLISLLERLMDPEKKVFRIVNILQALYELCAWEFPKTRRSTPQLRQLGLAPISLEADTEL 842

Query: 844  LFENAVVFPGLEDKYFYRNVQRLHTILTSRDSMHNVPSNLEARRRLAFFSNSLFMNMPRA 903
            LF NA+  P L+D  FYR ++R+HTILTSRD MHNVP N+EAR RLAFFSNSLFM MP+A
Sbjct: 843  LFVNAINLPPLDDVVFYRQIRRVHTILTSRDPMHNVPKNIEARERLAFFSNSLFMTMPQA 902

Query: 904  PYVEKMMPFSVLTPYYDEEVVYGKEMLRSENEDGVSTLFYLQRIYEDEWRNFMERMRKKG 963
            P VEKMM FSVLTPYYDEEV+Y +EMLR+ENEDG+STLFYLQRIYEDEW NF+ERMR++G
Sbjct: 903  PSVEKMMAFSVLTPYYDEEVMYRQEMLRAENEDGISTLFYLQRIYEDEWVNFLERMRREG 962

Query: 964  LEHEDDIWTKKSRELRLWASYRGQTLSRTVRGMMYYHRALKMFSFLDTASEMDIRMGSQE 1023
             E+E+DIW+KK R+LRLWASYRGQTLSRTVRGMMYY+ ALK  +FLD+ASEMDIRMG+Q 
Sbjct: 963  AENENDIWSKKVRDLRLWASYRGQTLSRTVRGMMYYYSALKKLAFLDSASEMDIRMGTQ- 1022

Query: 1024 IASHGSITRKHALDGLHSTQP-PSRNLNRASTG-ELLYRRSEYGTALMKFTYVVTCQVYG 1083
            IA     +      G ++ QP PS+ ++R ++G   L + SEYG+A+MKFTYVV CQVYG
Sbjct: 1023 IAPEARRSYYTNDGGDNTLQPTPSQEISRMASGITHLLKGSEYGSAMMKFTYVVACQVYG 1082

Query: 1084 LQKAKRDPRAEEILNLMKDNESLRVAYVDEVHRGRDEVEFYSVLVKYDQELKKEVVIYRI 1143
              KA+ D RAEEIL LMK++++LR+AYVDEV  GR EVE+YSVLVK+DQ+L++EV IYRI
Sbjct: 1083 QHKARGDHRAEEILFLMKNHDALRIAYVDEVDLGRGEVEYYSVLVKFDQQLQREVEIYRI 1142

Query: 1144 KLPGPLKIGEGKPENQNHAIIFTRGDAIQTIDMNQDNYFEEALKMRNLLEEFNNSYGIRK 1203
            +LPGPLK+GEGKPENQNHA+IFTRGDAIQTIDMNQDN+FEEALKMRNLLE F   YGIRK
Sbjct: 1143 RLPGPLKLGEGKPENQNHALIFTRGDAIQTIDMNQDNHFEEALKMRNLLESFKTYYGIRK 1202

Query: 1204 PTILGVRENVFTGSVSSLAWFMSAQETSFVTLAQRVLANPLKVRMHYGHPDVFDRFWFLT 1263
            PTILGVRE VFTGSVSSLAWFMSAQETSFVTL QRVLANPLKVRMHYGHPDVFDRFWF+ 
Sbjct: 1203 PTILGVREKVFTGSVSSLAWFMSAQETSFVTLGQRVLANPLKVRMHYGHPDVFDRFWFVP 1262

Query: 1264 RGGISKASKVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGFNQISMFEAKVASGN 1323
            RGGISKAS+VINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVG NQISMFEAKVASGN
Sbjct: 1263 RGGISKASRVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGLNQISMFEAKVASGN 1322

Query: 1324 GEQVLSRDIYRLGHRLDFFRVLSVFYTTVGYYFNTMLVVLSVYTFLWGRLYLALSGVEDA 1383
            GEQ LSRD+YRLGHRLDFFR+LS FYTTVGYYFNTML+V +VY FLWGRLYLALSGVE  
Sbjct: 1323 GEQALSRDVYRLGHRLDFFRMLSFFYTTVGYYFNTMLIVFTVYAFLWGRLYLALSGVEKI 1382

Query: 1384 AVASSSGNNRALGAILNQQFIIQLGLFTALPMIVENSLEHGFLPAIWNFLTMQLQLASFF 1443
            A   SS +N ALGAILNQQFIIQLGLFTALPMI+ENSLE GFLPA+W+F+TMQLQLASFF
Sbjct: 1383 AKDRSS-SNEALGAILNQQFIIQLGLFTALPMILENSLERGFLPAVWDFITMQLQLASFF 1442

Query: 1444 YTFSLGTRTHFFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFVKAIELGVILI 1503
            YTFS+GTRTH+FGRTILHGGAKYRATGRGFVV+HK FAENYRLYAR+HF+KAIEL +IL+
Sbjct: 1443 YTFSMGTRTHYFGRTILHGGAKYRATGRGFVVEHKKFAENYRLYARTHFIKAIELAIILL 1502

Query: 1504 VYASRSPLARSTFTFVILSISSWFLIVSWIMAPFIFNPSGFDWLKTVYDFDDFINWLWNA 1563
            VYA+ SPLA+S+F +++++ISSWFLI SWI++PF+FNPSGFDWLKTV DFDDFI WLW+ 
Sbjct: 1503 VYAAYSPLAKSSFVYILMTISSWFLITSWIISPFLFNPSGFDWLKTVNDFDDFIAWLWSR 1562

Query: 1564 GGVFTKAEQSWEAWWLEENSHLRSTGLWGKLLEIILDLRFFFFQYAIVYHLNITGNNTSI 1623
            GG+FTKA+QSW  WW EE  HL++TG+WGKLLEIILDLRFFFFQY+IVYHL I  N TSI
Sbjct: 1563 GGLFTKADQSWFTWWNEEQEHLKTTGVWGKLLEIILDLRFFFFQYSIVYHLRIAENRTSI 1622

Query: 1624 AVYFISWVSMIVLVGIYIVIAYARDKYAAKEHIYYRLVQLIVIVITVLVIVILMEFTPFN 1683
             VY ISW  +I +V IYI   YA+ +Y+ KEHI YR +Q +VI++TVLV+V++++FT   
Sbjct: 1623 GVYLISWGCIIGIVAIYITTIYAQKRYSVKEHIKYRFIQFLVILLTVLVVVMMLQFTKLT 1682

Query: 1684 TGDLVTCLLAFIPTGWGIISIAQVLRPFLQTTVVWDTVVSMARLYDLLFGVIAMAPLALL 1743
              DL+  LLAF+PTGWG+ISIAQVL+PFL +TVVWDTV+S+AR YDL FG+I MAP+ALL
Sbjct: 1683 VVDLLISLLAFVPTGWGLISIAQVLKPFLLSTVVWDTVISVARFYDLFFGLIVMAPVALL 1742

Query: 1744 SWLPGFQSMQTRILFNEAFSRGLQISRIIAGKKT 1769
            SWLPGFQ+MQTRILFNEAFSRGLQIS I+AGKK+
Sbjct: 1743 SWLPGFQNMQTRILFNEAFSRGLQISIILAGKKS 1767

BLAST of HG10021216 vs. TAIR 10
Match: AT4G03550.1 (glucan synthase-like 5 )

HSP 1 Score: 2381.7 bits (6171), Expect = 0.0e+00
Identity = 1168/1757 (66.48%), Postives = 1431/1757 (81.45%), Query Frame = 0

Query: 23   EPYNIIPIHDLLTDHPSLQSPEVRAAAAALRTVGELRRPSFVPWNPKYDLLDWLGLFFGF 82
            EPYNIIP+++LL DHPSL+ PEVRAAAAAL+TVG+LRRP +V W   YDLLDWL LFFGF
Sbjct: 26   EPYNIIPVNNLLADHPSLRFPEVRAAAAALKTVGDLRRPPYVQWRSHYDLLDWLALFFGF 85

Query: 83   QSDNVRNQREHLVLHLANSQMRLRSSPEQADLLDRTVLRNFRKKLLRSYTLWCSYLGRKS 142
            Q DNVRNQREH+VLHLAN+QMRL   P+  D LD  V+R FR+KLL +Y+ WCSYLG+KS
Sbjct: 86   QKDNVRNQREHMVLHLANAQMRLSPPPDNIDSLDSAVVRRFRRKLLANYSSWCSYLGKKS 145

Query: 143  NVRFPRRDQSEERRELLYVSLYLLIWGEAANLRFVPECLCYIYHFMAMELNQILDDYIDP 202
            N+    R+  + RRELLYV LYLLIWGEAANLRF+PEC+CYI+H MA ELN+IL+D +D 
Sbjct: 146  NIWISDRN-PDSRRELLYVGLYLLIWGEAANLRFMPECICYIFHNMASELNKILEDCLDE 205

Query: 203  ETGRPYSPAIHGDCAFLKSVVMPIYQTIKTEVESSRNGTAPHSAWRNYDDINEYFWSRRC 262
             TG+PY P++ G+ AFL  VV PIY TI+ E++ S+NGT  H  WRNYDDINEYFW+ RC
Sbjct: 206  NTGQPYLPSLSGENAFLTGVVKPIYDTIQAEIDESKNGTVAHCKWRNYDDINEYFWTDRC 265

Query: 263  FHNLGWPLELSSNFFATTPKNRRVGKTGFVEQRSFWNIFRSFDKIWILLLLFLQASIIVA 322
            F  L WPL+L SNFF +  + + VGKTGFVE+R+F+ ++RSFD++W++L LFLQA+IIVA
Sbjct: 266  FSKLKWPLDLGSNFFKS--RGKSVGKTGFVERRTFFYLYRSFDRLWVMLALFLQAAIIVA 325

Query: 323  WQGH-------QYPWIALKSRDVQVNLLTVFITWSGLRLFQAVLDAGTQYSLVSKETMWL 382
            W+         +  W ALK+RDVQV LLTVF+TWSG+RL QAVLDA +QY LVS+ET   
Sbjct: 326  WEEKPDTSSVTRQLWNALKARDVQVRLLTVFLTWSGMRLLQAVLDAASQYPLVSRETKRH 385

Query: 383  GVRMLLKGLAAIAWIIVFAVFYARIWSQKNSDGFWSDEANGKIFIFLRIVFAFVIPELLA 442
              RML+K +AA  WI+ F V Y  IW QK  D  WS+ A  KI+ FL  V AF++PE+LA
Sbjct: 386  FFRMLMKVIAAAVWIVAFTVLYTNIWKQKRQDRQWSNAATTKIYQFLYAVGAFLVPEILA 445

Query: 443  LLLFVLPWIRNGLEELDWKVMYLFTWWFHTRIFVGRGLREGLIDNIKYTIFWVAVLASKF 502
            L LF++PW+RN LEE +WK+ +  TWWF  + FVGRGLREGL+DNIKY+ FW+ VLA+KF
Sbjct: 446  LALFIIPWMRNFLEETNWKIFFALTWWFQGKSFVGRGLREGLVDNIKYSTFWIFVLATKF 505

Query: 503  SFSYFFQIQPLVGPTKGLLNLKG-PYKWHEFFGSTNIVAVVLLWTPVVLIYLMDLQIWYS 562
            +FSYF Q++P++ P+K L NLK   Y+WH+F+G +N  +V LLW PVVLIYLMD+QIWY+
Sbjct: 506  TFSYFLQVKPMIKPSKLLWNLKDVDYEWHQFYGDSNRFSVALLWLPVVLIYLMDIQIWYA 565

Query: 563  IFSSFVGAIVGLFLHLGEIRNIGQLRLRFQFFASAMQFNLMPEEQQLTPKLTRLKKIRDA 622
            I+SS VGA+VGLF HLGEIR++GQLRLRFQFFASA+QFNLMPEEQ L  +     K +D 
Sbjct: 566  IYSSIVGAVVGLFDHLGEIRDMGQLRLRFQFFASAIQFNLMPEEQLLNARGFG-NKFKDG 625

Query: 623  IHRLKLRYGLGQPYKKIESSMIDTTRFALIWNEILITMREEDLISDRDFDLLELPPNYWN 682
            IHRLKLRYG G+P+KK+ES+ ++  +FALIWNEI++  REED++SDR+ +LLELP N W+
Sbjct: 626  IHRLKLRYGFGRPFKKLESNQVEANKFALIWNEIILAFREEDIVSDREVELLELPKNSWD 685

Query: 683  IRVIRWPCFLLCNELLLALSQAIELGNKPDEDLWSKICKNEYQRCAVIEAYDSVKALLLN 742
            + VIRWPCFLLCNELLLALSQA EL + PD+ LW KICKNEY+RCAV+EAYDS+K LLL+
Sbjct: 686  VTVIRWPCFLLCNELLLALSQARELIDAPDKWLWHKICKNEYRRCAVVEAYDSIKHLLLS 745

Query: 743  IVKYGSEENSIVVKVFIDIDNVIGMGKFMEAYNPNVLQEIHTKLISLVELLIGTKKDLSK 802
            I+K  +EE+SI+   F  I+  I   +F + +  ++L +I+  L  LV L+   + D  +
Sbjct: 746  IIKVDTEEHSIITVFFQIINQSIQSEQFTKTFRVDLLPKIYETLQKLVGLVNDEETDSGR 805

Query: 803  AVFILQALYELSIREFPRSKKSTKQLREEGLVPRNPATDEGLLFENAVVFPGLEDKYFYR 862
             V +LQ+LYE++ R+F   KK+T+QL  EGL PR+PA+   LLF+NA+  P   ++ FYR
Sbjct: 806  VVNVLQSLYEIATRQFFIEKKTTEQLSNEGLTPRDPASK--LLFQNAIRLPDASNEDFYR 865

Query: 863  NVQRLHTILTSRDSMHNVPSNLEARRRLAFFSNSLFMNMPRAPYVEKMMPFSVLTPYYDE 922
             V+RLHTILTSRDSMH+VP NLEARRR+AFFSNSLFMNMP AP VEKMM FSVLTPYY E
Sbjct: 866  QVRRLHTILTSRDSMHSVPVNLEARRRIAFFSNSLFMNMPHAPQVEKMMAFSVLTPYYSE 925

Query: 923  EVVYGKEMLRSENEDGVSTLFYLQRIYEDEWRNFMERMRKKGLEHEDDIWTKKSRELRLW 982
            EVVY KE LR+E EDG+STL+YLQ IY DEW+NF ERM ++G++ + ++WT K R+LRLW
Sbjct: 926  EVVYSKEQLRNETEDGISTLYYLQTIYADEWKNFKERMHREGIKTDSELWTTKLRDLRLW 985

Query: 983  ASYRGQTLSRTVRGMMYYHRALKMFSFLDTASEMDIRMGSQEIASHGSITRK--HALDGL 1042
            ASYRGQTL+RTVRGMMYY+RALKM +FLD+ASEMDIR G+QE+ S  ++  +     DG 
Sbjct: 986  ASYRGQTLARTVRGMMYYYRALKMLAFLDSASEMDIREGAQELGSVRNLQGELGGQSDGF 1045

Query: 1043 HSTQPPSRNLNRASTGELLYRRSEYGTALMKFTYVVTCQVYGLQKAKRDPRAEEILNLMK 1102
             S    S     +S+   LY+  EYGTALMKFTYVV CQ+YG QKAK++P+AEEIL LMK
Sbjct: 1046 VSENDRSSLSRASSSVSTLYKGHEYGTALMKFTYVVACQIYGSQKAKKEPQAEEILYLMK 1105

Query: 1103 DNESLRVAYVDEVHRGRDEVEFYSVLVKYDQELKKEVVIYRIKLPGPLKIGEGKPENQNH 1162
             NE+LR+AYVDEV  GR E ++YSVLVKYD +L+KEV I+R+KLPGP+K+GEGKPENQNH
Sbjct: 1106 QNEALRIAYVDEVPAGRGETDYYSVLVKYDHQLEKEVEIFRVKLPGPVKLGEGKPENQNH 1165

Query: 1163 AIIFTRGDAIQTIDMNQDNYFEEALKMRNLLEEFNNSYGIRKPTILGVRENVFTGSVSSL 1222
            A+IFTRGDA+QTIDMNQD+YFEEALKMRNLL+E+N+ +GIRKPTILGVRE++FTGSVSSL
Sbjct: 1166 AMIFTRGDAVQTIDMNQDSYFEEALKMRNLLQEYNHYHGIRKPTILGVREHIFTGSVSSL 1225

Query: 1223 AWFMSAQETSFVTLAQRVLANPLKVRMHYGHPDVFDRFWFLTRGGISKASKVINISEDIF 1282
            AWFMSAQETSFVTL QRVLANPLKVRMHYGHPDVFDRFWFL+RGGISKAS+VINISEDIF
Sbjct: 1226 AWFMSAQETSFVTLGQRVLANPLKVRMHYGHPDVFDRFWFLSRGGISKASRVINISEDIF 1285

Query: 1283 AGFNCTLRGGNVTHHEYIQVGKGRDVGFNQISMFEAKVASGNGEQVLSRDIYRLGHRLDF 1342
            AGFNCTLRGGNVTHHEYIQVGKGRDVG NQISMFEAKVASGNGEQVLSRD+YRLGHRLDF
Sbjct: 1286 AGFNCTLRGGNVTHHEYIQVGKGRDVGLNQISMFEAKVASGNGEQVLSRDVYRLGHRLDF 1345

Query: 1343 FRVLSVFYTTVGYYFNTMLVVLSVYTFLWGRLYLALSGVEDAAVASSSGNNRALGAILNQ 1402
            FR+LS FYTTVG++FNTM+V+L+VY FLWGR+YLALSGVE +A+A S+  N ALG ILNQ
Sbjct: 1346 FRMLSFFYTTVGFFFNTMMVILTVYAFLWGRVYLALSGVEKSALADSTDTNAALGVILNQ 1405

Query: 1403 QFIIQLGLFTALPMIVENSLEHGFLPAIWNFLTMQLQLASFFYTFSLGTRTHFFGRTILH 1462
            QFIIQLGLFTALPMIVE SLE GFL AIWNF+ MQ+QL++ FYTFS+GTR H+FGRTILH
Sbjct: 1406 QFIIQLGLFTALPMIVEWSLEEGFLLAIWNFIRMQIQLSAVFYTFSMGTRAHYFGRTILH 1465

Query: 1463 GGAKYRATGRGFVVQHKSFAENYRLYARSHFVKAIELGVILIVYASRSPLARSTFTFVIL 1522
            GGAKYRATGRGFVV+HK F ENYRLYARSHFVKAIELG+ILIVYAS SP+A+ +  ++ +
Sbjct: 1466 GGAKYRATGRGFVVEHKGFTENYRLYARSHFVKAIELGLILIVYASHSPIAKDSLIYIAM 1525

Query: 1523 SISSWFLIVSWIMAPFIFNPSGFDWLKTVYDFDDFINWLWNAGGVFTKAEQSWEAWWLEE 1582
            +I+SWFL++SWIMAPF+FNPSGFDWLKTVYDF+DF+NW+W  G + TK+EQSWE WW EE
Sbjct: 1526 TITSWFLVISWIMAPFVFNPSGFDWLKTVYDFEDFMNWIWYQGRISTKSEQSWEKWWYEE 1585

Query: 1583 NSHLRSTGLWGKLLEIILDLRFFFFQYAIVYHLNITGNNTSIAVYFISWVSMIVLVGIYI 1642
              HLR+TG  G  +EIIL LRFFFFQY IVY L I   +TS+ VY  SW+ +  +  +++
Sbjct: 1586 QDHLRNTGKAGLFVEIILVLRFFFFQYGIVYQLKIANGSTSLFVYLFSWIYIFAIFVLFL 1645

Query: 1643 VIAYARDKYAAKEHIYYRLVQLIVIVITVLVIVILMEFTPFNTGDLVTCLLAFIPTGWGI 1702
            VI YARDKY+AK HI YRLVQ ++IV+ +LVIV L+EFT F+  D+ T LLAFIPTGWGI
Sbjct: 1646 VIQYARDKYSAKAHIRYRLVQFLLIVLAILVIVALLEFTHFSFIDIFTSLLAFIPTGWGI 1705

Query: 1703 ISIAQVLRPFLQT-TVVWDTVVSMARLYDLLFGVIAMAPLALLSWLPGFQSMQTRILFNE 1762
            + IAQ  R +L+  T+ W+ VVS+AR+YD+LFG++ M P+A LSW+PGFQSMQTRILFNE
Sbjct: 1706 LLIAQTQRKWLKNYTIFWNAVVSVARMYDILFGILIMVPVAFLSWMPGFQSMQTRILFNE 1765

Query: 1763 AFSRGLQISRIIAGKKT 1769
            AFSRGL+I +I+ GKK+
Sbjct: 1766 AFSRGLRIMQIVTGKKS 1776

BLAST of HG10021216 vs. TAIR 10
Match: AT2G13680.1 (callose synthase 5 )

HSP 1 Score: 1546.6 bits (4003), Expect = 0.0e+00
Identity = 845/1787 (47.29%), Postives = 1159/1787 (64.86%), Query Frame = 0

Query: 24   PYNIIPIHDLLTDHPSLQSPEVRAAAAALRTVGELRRPSFVPWNPK----YDLLDWLGLF 83
            PYNI+P+         +Q  EV+AA AAL     L  PS    + K     DLLDWL   
Sbjct: 188  PYNILPLDSAGASQSVMQLEEVKAAVAALGNTRGLNWPSGFEQHRKKTGNLDLLDWLRAM 247

Query: 84   FGFQSDNVRNQREHLVLHLANSQMRLRSSPEQADLLDRTVLRNFRKKLLRSYTLWCSYLG 143
            FGFQ DNVRNQREHLV   A++ +RL   PE  + LD   +     KL ++Y  WC +LG
Sbjct: 248  FGFQRDNVRNQREHLVCLFADNHIRLTPKPEPLNKLDDRAVDTVMSKLFKNYKNWCKFLG 307

Query: 144  RKSNVRFPRRDQSEERRELLYVSLYLLIWGEAANLRFVPECLCYIYHFMAMELNQILDDY 203
            RK ++R P+  Q  ++R++LY+ LYLLIWGEAAN+RF+PECLCYI+H MA EL+ +L   
Sbjct: 308  RKHSLRLPQAAQDIQQRKILYMGLYLLIWGEAANIRFMPECLCYIFHNMAYELHGLLAGN 367

Query: 204  IDPETGRPYSPAIHGDC-AFLKSVVMPIYQTIKTEVESSRNGTAPHSAWRNYDDINEYFW 263
            +   TG    P+  GD  AFL+ V+ PIY+ ++TE   + NG A HS W NYDD+NEYFW
Sbjct: 368  VSIVTGENIKPSYGGDDEAFLRKVITPIYRVVQTEANKNANGKAAHSDWSNYDDLNEYFW 427

Query: 264  SRRCFHNLGWPLELSSNFFATT-----------PKNRRVGKTGFVEQRSFWNIFRSFDKI 323
            +  CF +LGWP+    + F +T            K  R GK+ F E R+FW+I+ SFD++
Sbjct: 428  TPDCF-SLGWPMRDDGDLFKSTRDTTQGKKGSFRKAGRTGKSNFTETRTFWHIYHSFDRL 487

Query: 324  WILLLLFLQASIIVAWQGHQYPWIALKSRDVQVNLLTVFITWSGLRLFQAVLDAGTQYSL 383
            W   LL LQA II+A++  +   I  K  DV   L ++FIT + LR  Q+VLD    +  
Sbjct: 488  WTFYLLALQAMIILAFERVELREILRK--DVLYALSSIFITAAFLRFLQSVLDVILNFPG 547

Query: 384  VSKETMWLGVRMLLKGLAAIAWIIVFAVFYARIWS----QKNSDGFWSDEANGKIFIFLR 443
              +      +R +LK + ++AW +V  + YA+  S    +      +  +  G   +++ 
Sbjct: 548  FHRWKFTDVLRNILKIVVSLAWCVVLPLCYAQSVSFAPGKLKQWLSFLPQVKGVPPLYIM 607

Query: 444  IVFAFVIPELLALLLFVLPWIRNGLEELDWKVMYLFTWWFHTRIFVGRGLREGLIDNIKY 503
             V  +++P +LA ++F+ P +R  +E  DW +  L  WW   RI+VGRG+ E  I  IKY
Sbjct: 608  AVALYLLPNVLAAIMFIFPMLRRWIENSDWHIFRLLLWWSQPRIYVGRGMHESQIALIKY 667

Query: 504  TIFWVAVLASKFSFSYFFQIQPLVGPTKGLLNLKG-PYKWHEFFGST--NIVAVVLLWTP 563
            TIFW+ +   KF+FSYF Q++ LV PT  +++++   YKWHEFF +   N  AVV LW P
Sbjct: 668  TIFWLLLFCCKFAFSYFLQVKLLVKPTNAIMSIRHVKYKWHEFFPNAEHNYGAVVSLWLP 727

Query: 564  VVLIYLMDLQIWYSIFSSFVGAIVGLFLHLGEIRNIGQLRLRFQFFASAMQFNLMPEEQQ 623
            V+L+Y MD QIWY+IFS+  G ++G F  LGEIR +G LR RFQ    A    L+P +  
Sbjct: 728  VILVYFMDTQIWYAIFSTICGGVIGAFDRLGEIRTLGMLRSRFQSLPGAFNTYLVPSD-- 787

Query: 624  LTPKLTRLKKIRDAIHRLKLRYGLGQPYKKIESSMIDTTRFALIWNEILITMREEDLISD 683
                     K R     L  R+      ++ E++     +F+ +WNEI+ + REEDLISD
Sbjct: 788  ---------KTRRRGFSLSKRFAEVTAARRTEAA-----KFSQLWNEIISSFREEDLISD 847

Query: 684  RDFDLLELP-PNYWNIRVIRWPCFLLCNELLLALSQAIELGNKPDEDLWSKICKNEYQRC 743
            R+ DLL +P  +  ++++I+WP FLL +++ +AL  A +   + D DLW +IC +EY +C
Sbjct: 848  REMDLLLVPYTSDPSLKLIQWPPFLLASKIPIALDMAAQFRTR-DSDLWKRICADEYMKC 907

Query: 744  AVIEAYDSVKALLLNIVKYGSEENSIVVKVFIDIDNVIGMGKFMEAYNPNVLQEIHTKLI 803
            AVIE Y+S K +L  +V  G  E  I+  +  ++++ I    F+  +    L  + +K +
Sbjct: 908  AVIECYESFKHVLHTLV-IGENEKRIIGIIIKEVESNISKNSFLSNFRMAPLPALCSKFV 967

Query: 804  SLVELLIG---TKKDLSKAVFILQALYELSIREFPRSKKSTKQLREEGLVPRNPATD--E 863
             LV +L      K+D    V +LQ + E+  R+  +++   ++L E G   +        
Sbjct: 968  ELVGILKNADPAKRD--TVVLLLQDMLEVVTRDMMQNE--NRELVELGHTNKESGRQLFA 1027

Query: 864  GLLFENAVVFPGLEDKYFYRNVQRLHTILTSRDSMHNVPSNLEARRRLAFFSNSLFMNMP 923
            G   + A++FP +    ++  + RLH +LT ++S  +VP+NLEA+RR+AFF+NSLFM+MP
Sbjct: 1028 GTDAKPAILFPPVATAQWHEQISRLHLLLTVKESAMDVPTNLEAQRRIAFFTNSLFMDMP 1087

Query: 924  RAPYVEKMMPFSVLTPYYDEEVVYGKEMLRSENEDGVSTLFYLQRIYEDEWRNFMERMRK 983
            RAP V  M+ FSVLTPYY EE VY K  L  ENEDGVS ++YLQ+I+ DEW NF+ER+  
Sbjct: 1088 RAPRVRNMLSFSVLTPYYSEETVYSKNDLEMENEDGVSVVYYLQKIFPDEWTNFLERLDC 1147

Query: 984  KG----LEHEDDIWTKKSRELRLWASYRGQTLSRTVRGMMYYHRALKMFSFLDTASEMDI 1043
            K     LE E++I      +LR W S RGQTL RTVRGMMYY RALK+ +FLD A+E +I
Sbjct: 1148 KDETSVLESEENI-----LQLRHWVSLRGQTLFRTVRGMMYYRRALKLQAFLDMANETEI 1207

Query: 1044 RMGSQEIASHGSITRKHALDGLHSTQPPSRNLNRASTGELLYRRSEYGTALMKFTYVVTC 1103
              G + I+      +K                    +   LY + E   A +KFTYV TC
Sbjct: 1208 LAGYKAISEPTEEDKK--------------------SQRSLYTQLE-AVADLKFTYVATC 1267

Query: 1104 QVYGLQKAKRDPRAEEILNLMKDNESLRVAYVDEVHR---GRDEVEFYSVLVKYDQELKK 1163
            Q YG QK   D RA +ILNLM +N SLRVAY+DEV     G+ +  FYSVL+K    L +
Sbjct: 1268 QNYGNQKRSGDRRATDILNLMVNNPSLRVAYIDEVEEREGGKVQKVFYSVLIKAVDNLDQ 1327

Query: 1164 EVVIYRIKLPGPLKIGEGKPENQNHAIIFTRGDAIQTIDMNQDNYFEEALKMRNLLEEFN 1223
            E  IYRIKLPGP KIGEGKPENQNHA+IFTRG+A+Q IDMNQD+Y EEALKMRNLLEEFN
Sbjct: 1328 E--IYRIKLPGPAKIGEGKPENQNHALIFTRGEALQAIDMNQDHYLEEALKMRNLLEEFN 1387

Query: 1224 NSYGIRKPTILGVRENVFTGSVSSLAWFMSAQETSFVTLAQRVLANPLKVRMHYGHPDVF 1283
              +G+R PTILG RE++FTGSVSSLAWFMS QETSFVT+ QRVLA+PLKVR HYGHPDVF
Sbjct: 1388 EDHGVRAPTILGFREHIFTGSVSSLAWFMSNQETSFVTIGQRVLASPLKVRFHYGHPDVF 1447

Query: 1284 DRFWFLTRGGISKASKVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGFNQISMFE 1343
            DR + +TRGGISKAS+ IN+SEDIFAGFN TLR GNVTHHEYIQVGKGRDVG NQIS+FE
Sbjct: 1448 DRIFHITRGGISKASRGINLSEDIFAGFNSTLRRGNVTHHEYIQVGKGRDVGLNQISLFE 1507

Query: 1344 AKVASGNGEQVLSRDIYRLGHRLDFFRVLSVFYTTVGYYFNTMLVVLSVYTFLWGRLYLA 1403
            AKVA GNGEQ LSRD+YRLGHR DFFR++S ++TTVG+Y ++M+VVL+VY FL+GRLYL+
Sbjct: 1508 AKVACGNGEQTLSRDLYRLGHRFDFFRMMSCYFTTVGFYISSMIVVLTVYAFLYGRLYLS 1567

Query: 1404 LSGVEDAAV--ASSSGNNRALGAILNQQFIIQLGLFTALPMIVENSLEHGFLPAIWNFLT 1463
            LSGVE+A V  A++ G++ +L A +  Q ++QLGL   LPM++E  LE GF  A+ + + 
Sbjct: 1568 LSGVEEAIVKFAAAKGDS-SLKAAMASQSVVQLGLLMTLPMVMEIGLERGFRTALSDLII 1627

Query: 1464 MQLQLASFFYTFSLGTRTHFFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFVK 1523
            MQLQLA  F+TFSLGT+ H++GRTILHGG+KYRATGRGFVV+H+ FAENYR+Y+RSHFVK
Sbjct: 1628 MQLQLAPVFFTFSLGTKVHYYGRTILHGGSKYRATGRGFVVKHEKFAENYRMYSRSHFVK 1687

Query: 1524 AIELGVILIVYASRSPLARSTFTFVILSISSWFLIVSWIMAPFIFNPSGFDWLKTVYDFD 1583
             +EL V+LI Y      A  +  + ++  S+WFL+ SW+ APF FNPSGF+W K V D+D
Sbjct: 1688 GMELMVLLICYRIYGKAAEDSVGYALVMGSTWFLVGSWLFAPFFFNPSGFEWQKIVDDWD 1747

Query: 1584 DFINWLWNAGGVFTKAEQSWEAWWLEENSHLRSTGLWGKLLEIILDLRFFFFQYAIVYHL 1643
            D+  W+ + GG+   A +SWE+WW EE  HL  +G +GK  EI L LR+F +QY IVY L
Sbjct: 1748 DWNKWISSRGGIGVPANKSWESWWEEEQEHLLHSGFFGKFWEIFLSLRYFIYQYGIVYQL 1807

Query: 1644 NIT-----GNNTSIAVYFISWVSMIVLVGIYIVIAYARDKYAAKEHIYYRLVQLIVIVIT 1703
            N+T     G   SI VY +SW+ ++ ++ +  +++  R K++A   + +RL++L + + +
Sbjct: 1808 NLTKESRMGKQHSIIVYGLSWLVIVAVMIVLKIVSMGRKKFSADFQLMFRLLKLFLFIGS 1867

Query: 1704 VLVIVILMEFTPFNTGDLVTCLLAFIPTGWGIISIAQVLRPFLQTTVVWDTVVSMARLYD 1763
            V+++ +L  F     GD++  LLAF+PTGW ++ I+QV RP ++T  +W +V ++AR Y+
Sbjct: 1868 VVIVGMLFHFLKLTVGDIMQSLLAFLPTGWALLQISQVARPLMKTVGMWGSVKALARGYE 1920

Query: 1764 LLFGVIAMAPLALLSWLPGFQSMQTRILFNEAFSRGLQISRIIAGKK 1768
             + GV+   P+ +L+W P     QTR+LFN+AFSRGLQI RI+AG K
Sbjct: 1928 YIMGVVIFMPVTVLAWFPFVSEFQTRLLFNQAFSRGLQIQRILAGGK 1920

BLAST of HG10021216 vs. TAIR 10
Match: AT1G05570.1 (callose synthase 1 )

HSP 1 Score: 1541.2 bits (3989), Expect = 0.0e+00
Identity = 841/1805 (46.59%), Postives = 1150/1805 (63.71%), Query Frame = 0

Query: 24   PYNIIPIHDLLTDHPSLQSPEVRAAAAALRTVGELRRPSFVPWNPKY------DLLDWLG 83
            PYNI+P+     +   ++ PE++AA AALR    L      PW   +      D+LDWL 
Sbjct: 185  PYNILPLDPDSQNQAIMRLPEIQAAVAALRNTRGL------PWTAGHKKKLDEDILDWLQ 244

Query: 84   LFFGFQSDNVRNQREHLVLHLANSQMRLRSSPEQADLLDRTVLRNFRKKLLRSYTLWCSY 143
              FGFQ DNV NQREHL+L LAN  +R    P+Q   LD   L    KKL R+Y  WC Y
Sbjct: 245  SMFGFQKDNVLNQREHLILLLANVHIRQFPKPDQQPKLDDRALTIVMKKLFRNYKKWCKY 304

Query: 144  LGRKSNVRFPRRDQSEERRELLYVSLYLLIWGEAANLRFVPECLCYIYHFMAMELNQILD 203
            LGRKS++  P   Q  ++R+LLY+ LYLLIWGEAANLRF+PECLCYIYH MA EL  +L 
Sbjct: 305  LGRKSSLWLPTIQQEVQQRKLLYMGLYLLIWGEAANLRFMPECLCYIYHHMAFELYGMLA 364

Query: 204  DYIDPETGRPYSPAIHG-DCAFLKSVVMPIYQTIKTEVESSRNGTAPHSAWRNYDDINEY 263
              + P TG    PA  G D AFL+ VV PIYQTI  E + SR G + HS WRNYDD+NEY
Sbjct: 365  GSVSPMTGEHVKPAYGGEDEAFLQKVVTPIYQTISKEAKRSRGGKSKHSVWRNYDDLNEY 424

Query: 264  FWSRRCFHNLGWPLELSSNFFATTPK--------------NRRVGKTGFVEQRSFWNIFR 323
            FWS RCF  LGWP+   ++FF  T +              +R +GK  FVE RSFW+IFR
Sbjct: 425  FWSIRCF-RLGWPMRADADFFCQTAEELRLERSEIKSNSGDRWMGKVNFVEIRSFWHIFR 484

Query: 324  SFDKIWILLLLFLQASIIVAWQGHQYPWIALKSRDVQVNLLTVFITWSGLRLFQAVLDAG 383
            SFD++W   +L LQA I++AW G      A+   DV + +L+VFIT + L+L QAVLD  
Sbjct: 485  SFDRLWSFYILCLQAMIVIAWNG-SGELSAIFQGDVFLKVLSVFITAAILKLAQAVLDIA 544

Query: 384  TQYSLVSKETMWLGVRMLLKGLAAIAWIIVFAVFYARIWSQKNSDGF-------WSDEAN 443
              +      ++++ +R ++K  AA  W++V AV YA  W  KN+ GF       +   ++
Sbjct: 545  LSWKARHSMSLYVKLRYVMKVGAAAVWVVVMAVTYAYSW--KNASGFSQTIKNWFGGHSH 604

Query: 444  GKIFIFLRIVFAFVIPELLALLLFVLPWIRNGLEELDWKVMYLFTWWFHTRIFVGRGLRE 503
                +F+  +  ++ P +L+ LLF+ P+IR  LE  D+K+M L  WW   R+++GRG+ E
Sbjct: 605  NSPSLFIVAILIYLSPNMLSALLFLFPFIRRYLERSDYKIMMLMMWWSQPRLYIGRGMHE 664

Query: 504  GLIDNIKYTIFWVAVLASKFSFSYFFQIQPLVGPTKGLLNLK-GPYKWHEFF--GSTNIV 563
              +   KYT+FW+ +L SK +FSY+ +I+PLVGPTK ++ +    Y WHEFF     N+ 
Sbjct: 665  SALSLFKYTMFWIVLLISKLAFSYYAEIKPLVGPTKDIMRIHISVYSWHEFFPHAKNNLG 724

Query: 564  AVVLLWTPVVLIYLMDLQIWYSIFSSFVGAIVGLFLHLGEIRNIGQLRLRFQFFASAMQF 623
             V+ LW+PV+L+Y MD QIWY+I S+ VG + G F  LGEIR +G LR RFQ    A   
Sbjct: 725  VVIALWSPVILVYFMDTQIWYAIVSTLVGGLNGAFRRLGEIRTLGMLRSRFQSIPGAFND 784

Query: 624  NLMPEEQQLTPKLTRLKKIRDAIHRLKLRYGLGQPYKKIESSM-IDTTRFALIWNEILIT 683
             L+P++              D   + + R    + + ++ SS   +  RFA +WN+I+ +
Sbjct: 785  CLVPQDNS------------DDTKKKRFRATFSRKFDQLPSSKDKEAARFAQMWNKIISS 844

Query: 684  MREEDLISDRDFDLLELPPNYW---NIRVIRWPCFLLCNELLLALSQAIELGNKPDEDLW 743
             REEDLISDR+ +LL +P  YW   ++ +IRWP FLL +++ +AL  A +  N  D +L 
Sbjct: 845  FREEDLISDREMELLLVP--YWSDPDLDLIRWPPFLLASKIPIALDMAKD-SNGKDRELK 904

Query: 744  SKICKNEYQRCAVIEAYDSVKALLLNIVKYGSEENSIVVKVFIDIDNVIGMGKFMEAYNP 803
             ++  + Y  CAV E Y S K  L+N +  G  E  ++  +F  ID  I     +   N 
Sbjct: 905  KRLAVDSYMTCAVRECYASFKN-LINYLVVGEREGQVINDIFSKIDEHIEKETLITELNL 964

Query: 804  NVLQEIHTKLISLVE-LLIGTKKDLSKAVFILQALYELSIREFPRSK-KSTKQLREEGLV 863
            + L +++ + + L+E LL   ++D  + V +L  + EL  R+    +  S  +    G  
Sbjct: 965  SALPDLYGQFVRLIEYLLENREEDKDQIVIVLLNMLELVTRDIMEEEVPSLLETAHNGSY 1024

Query: 864  PR----NPATDEGLLFENAVVFPGLEDKYFYRNVQRLHTILTSRDSMHNVPSNLEARRRL 923
             +     P   +   F         + + +   ++RLH +LT ++S  +VPSNLEARRRL
Sbjct: 1025 VKYDVMTPLHQQRKYFSQLRFPVYSQTEAWKEKIKRLHLLLTVKESAMDVPSNLEARRRL 1084

Query: 924  AFFSNSLFMNMPRAPYVEKMMPFSVLTPYYDEEVVYGKEMLRSENEDGVSTLFYLQRIYE 983
             FFSNSLFM+MP AP +  M+ FSVLTPY+ E+V++    L  +NEDGVS LFYLQ+I+ 
Sbjct: 1085 TFFSNSLFMDMPPAPKIRNMLSFSVLTPYFSEDVLFSIFGLEQQNEDGVSILFYLQKIFP 1144

Query: 984  DEWRNFMERMRKKGLEHEDDIWTKKSRELRLWASYRGQTLSRTVRGMMYYHRALKMFSFL 1043
            DEW NF+ER+ K G E E         ELRLWASYRGQTL++TVRGMMYY +AL++ +FL
Sbjct: 1145 DEWTNFLERV-KCGNEEELRAREDLEEELRLWASYRGQTLTKTVRGMMYYRKALELQAFL 1204

Query: 1044 DTASEMDIRMGSQEIASHGSITRKHALDGLHSTQPPSRNLNRASTGELLYRRSEYGTALM 1103
            D A + ++  G + +     +T + A                + +G  L+ + +   A M
Sbjct: 1205 DMAKDEELLKGYKAL----ELTSEEA----------------SKSGGSLWAQCQ-ALADM 1264

Query: 1104 KFTYVVTCQVYGLQKAKRDPRAEEILNLMKDNESLRVAYVDEV-------HRGRDEVEFY 1163
            KFT+VV+CQ Y + K   D RA++IL LM    S+RVAY+DEV       ++G +E  +Y
Sbjct: 1265 KFTFVVSCQQYSIHKRSGDQRAKDILRLMTTYPSIRVAYIDEVEQTHKESYKGTEEKIYY 1324

Query: 1164 SVLVKYDQELKK----------EVVIYRIKLPGPLKIGEGKPENQNHAIIFTRGDAIQTI 1223
            S LVK   + K           + +IYRIKLPGP  +GEGKPENQNHAIIFTRG+ +QTI
Sbjct: 1325 SALVKAAPQTKPMDSSESVQTLDQLIYRIKLPGPAILGEGKPENQNHAIIFTRGEGLQTI 1384

Query: 1224 DMNQDNYFEEALKMRNLLEEFNNSY-GIRKPTILGVRENVFTGSVSSLAWFMSAQETSFV 1283
            DMNQDNY EEA KMRNLL+EF   + G+R PTILG+RE++FTGSVSSLAWFMS QE SFV
Sbjct: 1385 DMNQDNYMEEAFKMRNLLQEFLEKHGGVRCPTILGLREHIFTGSVSSLAWFMSNQENSFV 1444

Query: 1284 TLAQRVLANPLKVRMHYGHPDVFDRFWFLTRGGISKASKVINISEDIFAGFNCTLRGGNV 1343
            T+ QRVLA+PLKVR HYGHPD+FDR + LTRGGI KASKVIN+SEDIFAGFN TLR GNV
Sbjct: 1445 TIGQRVLASPLKVRFHYGHPDIFDRLFHLTRGGICKASKVINLSEDIFAGFNSTLREGNV 1504

Query: 1344 THHEYIQVGKGRDVGFNQISMFEAKVASGNGEQVLSRDIYRLGHRLDFFRVLSVFYTTVG 1403
            THHEYIQVGKGRDVG NQISMFEAK+A+GNGEQ LSRD+YRLGHR DFFR+LS ++TT+G
Sbjct: 1505 THHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRDLYRLGHRFDFFRMLSCYFTTIG 1564

Query: 1404 YYFNTMLVVLSVYTFLWGRLYLALSGVEDAAVASSS-GNNRALGAILNQQFIIQLGLFTA 1463
            +YF+TML VL+VY FL+GRLYL LSG+E+   +  +  NN+ L A L  Q  +Q+G   A
Sbjct: 1565 FYFSTMLTVLTVYVFLYGRLYLVLSGLEEGLSSQRAFRNNKPLEAALASQSFVQIGFLMA 1624

Query: 1464 LPMIVENSLEHGFLPAIWNFLTMQLQLASFFYTFSLGTRTHFFGRTILHGGAKYRATGRG 1523
            LPM++E  LE GF  A+  F+ MQLQLAS F+TF LGT+TH++GRT+ HGGA+YR TGRG
Sbjct: 1625 LPMMMEIGLERGFHNALIEFVLMQLQLASVFFTFQLGTKTHYYGRTLFHGGAEYRGTGRG 1684

Query: 1524 FVVQHKSFAENYRLYARSHFVKAIELGVILIVYASRSPLARSTFTFVILSISSWFLIVSW 1583
            FVV H  FAENYR Y+RSHFVK IEL ++L+VY       R   T++++++S WF++V+W
Sbjct: 1685 FVVFHAKFAENYRFYSRSHFVKGIELMILLLVYQIFGQSYRGVVTYILITVSIWFMVVTW 1744

Query: 1584 IMAPFIFNPSGFDWLKTVYDFDDFINWLWNAGGVFTKAEQSWEAWWLEENSHLRSTGLWG 1643
            + APF+FNPSGF+W K V D+ D+  W++N GG+    E+SWE+WW +E  HLR +G+ G
Sbjct: 1745 LFAPFLFNPSGFEWQKIVDDWTDWNKWIYNRGGIGVPPEKSWESWWEKELEHLRHSGVRG 1804

Query: 1644 KLLEIILDLRFFFFQYAIVYHLN-ITGNNTSIAVYFISWVSMIVLVGIYIVIAYARDKYA 1703
              LEI L LRFF FQY +VYHL+   G N S  VY  SW  ++ ++ I   +   R +++
Sbjct: 1805 ITLEIFLALRFFIFQYGLVYHLSTFKGKNQSFWVYGASWFVILFILLIVKGLGVGRRRFS 1864

Query: 1704 AKEHIYYRLVQLIVIVITVLVIVILMEFTPFNTGDLVTCLLAFIPTGWGIISIAQVLRPF 1763
                + +R+++ +V +  V +++  +        DL  C+LAF+PTGWG++ IAQ  +P 
Sbjct: 1865 TNFQLLFRIIKGLVFLTFVAILITFLALPLITIKDLFICMLAFMPTGWGMLLIAQACKPL 1924

Query: 1764 LQTTVVWDTVVSMARLYDLLFGVIAMAPLALLSWLPGFQSMQTRILFNEAFSRGLQISRI 1768
            +Q   +W +V ++AR Y+++ G++   P+A L+W P     QTR+LFN+AFSRGLQISRI
Sbjct: 1925 IQQLGIWSSVRTLARGYEIVMGLLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRI 1941

BLAST of HG10021216 vs. TAIR 10
Match: AT5G13000.1 (glucan synthase-like 12 )

HSP 1 Score: 1532.7 bits (3967), Expect = 0.0e+00
Identity = 832/1807 (46.04%), Postives = 1155/1807 (63.92%), Query Frame = 0

Query: 24   PYNIIPIHDLLTDHPSLQSPEVRAAAAALRTVGELRRPSFVPWNPKYDLLDWLGLFFGFQ 83
            PYNI+P+     +   ++ PE++AA  ALR    L  P         D+LDWL   FGFQ
Sbjct: 189  PYNILPLDPDSANQAIMRYPEIQAAVLALRNTRGLPWPEGHKKKKDEDMLDWLQEMFGFQ 248

Query: 84   SDNVRNQREHLVLHLANSQMRLRSSPEQADLLDRTVLRNFRKKLLRSYTLWCSYLGRKSN 143
             DNV NQREHL+L LAN  +R    P+Q   LD   L    KKL ++Y  WC YLGRKS+
Sbjct: 249  KDNVANQREHLILLLANVHIRQFPKPDQQPKLDDQALTEVMKKLFKNYKKWCKYLGRKSS 308

Query: 144  VRFPRRDQSEERRELLYVSLYLLIWGEAANLRFVPECLCYIYHFMAMELNQILDDYIDPE 203
            +  P   Q  ++R+LLY++LYLLIWGEAANLRF+PECLCYIYH MA EL  +L   + P 
Sbjct: 309  LWLPTIQQEMQQRKLLYMALYLLIWGEAANLRFMPECLCYIYHHMAFELYGMLAGNVSPM 368

Query: 204  TGRPYSPAIHG-DCAFLKSVVMPIYQTIKTEVESSRNGTAPHSAWRNYDDINEYFWSRRC 263
            TG    PA  G + AFL+ VV PIY+ I+ E + S+ G + HS WRNYDD+NEYFWS  C
Sbjct: 369  TGENVKPAYGGEEDAFLRKVVTPIYEVIQMEAQRSKKGKSKHSQWRNYDDLNEYFWSVDC 428

Query: 264  FHNLGWPLELSSNFFA-----------------TTPKNRRVGKTGFVEQRSFWNIFRSFD 323
            F  LGWP+   ++FF                     ++R VGK  FVE RSFW++FRSFD
Sbjct: 429  F-RLGWPMRADADFFCLPVAVPNTEKDGDNSKPIVARDRWVGKVNFVEIRSFWHVFRSFD 488

Query: 324  KIWILLLLFLQASIIVAWQGHQYPWIALKSRDVQVNLLTVFITWSGLRLFQAVLDAGTQY 383
            ++W   +L LQA II+AW G Q    ++   DV   +L+VFIT + ++L QAVLD    +
Sbjct: 489  RMWSFYILCLQAMIIMAWDGGQPS--SVFGADVFKKVLSVFITAAIMKLGQAVLDVILNF 548

Query: 384  SLVSKETMWLGVRMLLKGLAAIAWIIVFAVFYARIWSQKNSDGF------WSDEANGKIF 443
                  T+ + +R +LK  +A AW+I+  V YA  W  K+   F      W   A     
Sbjct: 549  KAHQSMTLHVKLRYILKVFSAAAWVIILPVTYAYSW--KDPPAFARTIKSWFGSAMHSPS 608

Query: 444  IFLRIVFAFVIPELLALLLFVLPWIRNGLEELDWKVMYLFTWWFHTRIFVGRGLREGLID 503
            +F+  V +++ P +LA ++F+ P +R  LE  +++++ L  WW   R++VGRG+ E    
Sbjct: 609  LFIIAVVSYLSPNMLAGVMFLFPLLRRFLERSNYRIVMLMMWWSQPRLYVGRGMHESAFS 668

Query: 504  NIKYTIFWVAVLASKFSFSYFFQIQPLVGPTKGLLNLK-GPYKWHEFF--GSTNIVAVVL 563
              KYT+FWV ++A+K +FSY+ +I+PLV PT+ ++  +   ++WHEFF     NI  V+ 
Sbjct: 669  LFKYTMFWVLLIATKLAFSYYIEIRPLVAPTQAIMKARVTNFQWHEFFPRAKNNIGVVIA 728

Query: 564  LWTPVVLIYLMDLQIWYSIFSSFVGAIVGLFLHLGEIRNIGQLRLRFQFFASAMQFNLMP 623
            LW P++L+Y MD QIWY+IFS+  G I G F  LGEIR +G LR RF+    A    L+P
Sbjct: 729  LWAPIILVYFMDSQIWYAIFSTLFGGIYGAFRRLGEIRTLGMLRSRFESLPGAFNDRLIP 788

Query: 624  EEQQLTPKLTRLKKIRDAIHRLKLRYGLGQPYKKIESSMIDTTRFALIWNEILITMREED 683
            + +    K    K I     R  L +   +    +     +  RFA +WN I+ + REED
Sbjct: 789  DGKNQQKK----KGI-----RATLSHNFTEDKVPVNKEK-EAARFAQLWNTIISSFREED 848

Query: 684  LISDRDFDLLELPPNYW---NIRVIRWPCFLLCNELLLALSQAIELGNKPDEDLWSKICK 743
            LISDR+ DLL +P  YW   ++ +I+WP FLL +++ +AL  A +  N  D +L  +I  
Sbjct: 849  LISDREMDLLLVP--YWADRDLDLIQWPPFLLASKIPIALDMAKD-SNGKDRELKKRIES 908

Query: 744  NEYQRCAVIEAYDSVKALLLNIVKYGSEENSIVVKVFIDIDNVIGMGKFMEAYNPNVLQE 803
            + Y +CAV E Y S K ++  +V+ G+ E  ++  +F ++D  I  G  ++ Y  + L  
Sbjct: 909  DTYMKCAVRECYASFKNIIKFVVQ-GNREKEVIEIIFAEVDKHIDTGDLIQEYKMSALPS 968

Query: 804  IHTKLISLVELLIGTK-KDLSKAVFILQALYELSIREFPRSKKSTKQLREE--------G 863
            ++   + L++ L+  K +D    V + Q + E+  R+      +   L +         G
Sbjct: 969  LYDHFVKLIKYLLDNKEEDRDHVVILFQDMLEVVTRDIMMEDYNISSLVDSSHGGTWHGG 1028

Query: 864  LVPRNPATDEGLLF--ENAVVFPGLE--DKYFYRNVQRLHTILTSRDSMHNVPSNLEARR 923
            ++   P   +  LF    A+ FP +E   + +   ++R++ +LT+++S  +VPSNLEARR
Sbjct: 1029 MI---PLEQQYQLFASSGAIRFP-IEPVTEAWKEKIKRIYLLLTTKESAMDVPSNLEARR 1088

Query: 924  RLAFFSNSLFMNMPRAPYVEKMMPFSVLTPYYDEEVVYGKEMLRSENEDGVSTLFYLQRI 983
            R++FFSNSLFM+MP AP V  M+ FSVLTPYY EEV++    L + NEDGVS LFYLQ+I
Sbjct: 1089 RISFFSNSLFMDMPMAPKVRNMLSFSVLTPYYTEEVLFSLRDLETPNEDGVSILFYLQKI 1148

Query: 984  YEDEWRNFMERMR---KKGLEHEDDIWTKKSRELRLWASYRGQTLSRTVRGMMYYHRALK 1043
            + DEW NF+ER++   ++ L+  D++      ELRLWASYRGQTL+RTVRGMMYY +AL+
Sbjct: 1149 FPDEWNNFLERVKCLSEEELKESDEL----EEELRLWASYRGQTLTRTVRGMMYYRKALE 1208

Query: 1044 MFSFLDTASEMDIRMGSQEIASHGSITRKHALDGLHSTQPPSRNLNRASTGELLYRRSEY 1103
            + +FLD A   D+                  ++G  + +  S N +R   GE        
Sbjct: 1209 LQAFLDMAMHEDL------------------MEGYKAVELNSENNSR---GERSLWAQCQ 1268

Query: 1104 GTALMKFTYVVTCQVYGLQKAKRDPRAEEILNLMKDNESLRVAYVDEVH--------RGR 1163
              A MKFTYVV+CQ YG+ K   DPRA++IL LM    SLRVAY+DEV         +G 
Sbjct: 1269 AVADMKFTYVVSCQQYGIHKRSGDPRAQDILRLMTRYPSLRVAYIDEVEEPVKDKSKKGN 1328

Query: 1164 DEVEFYSVLVKYDQEL-------KKEVVIYRIKLPGPLKIGEGKPENQNHAIIFTRGDAI 1223
             +V +YSVLVK  +           + VIYRI+LPGP  +GEGKPENQNHAIIF+RG+ +
Sbjct: 1329 QKV-YYSVLVKVPKSTDHSTLAQNLDQVIYRIRLPGPAILGEGKPENQNHAIIFSRGEGL 1388

Query: 1224 QTIDMNQDNYFEEALKMRNLLEEFNNSY-GIRKPTILGVRENVFTGSVSSLAWFMSAQET 1283
            QTIDMNQDNY EEALKMRNLL+EF   + G+R P+ILG+RE++FTGSVSSLAWFMS QET
Sbjct: 1389 QTIDMNQDNYMEEALKMRNLLQEFLTKHDGVRHPSILGLREHIFTGSVSSLAWFMSNQET 1448

Query: 1284 SFVTLAQRVLANPLKVRMHYGHPDVFDRFWFLTRGGISKASKVINISEDIFAGFNCTLRG 1343
            SFVT+ QR+LANPL+VR HYGHPDVFDR + LTRGG+SKASKVIN+SEDIFAGFN TLR 
Sbjct: 1449 SFVTIGQRLLANPLRVRFHYGHPDVFDRLFHLTRGGVSKASKVINLSEDIFAGFNSTLRE 1508

Query: 1344 GNVTHHEYIQVGKGRDVGFNQISMFEAKVASGNGEQVLSRDIYRLGHRLDFFRVLSVFYT 1403
            GNVTHHEYIQVGKGRDVG NQISMFEAK+A+GNGEQ LSRDIYRLGHR DFFR++S ++T
Sbjct: 1509 GNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRDIYRLGHRFDFFRMMSCYFT 1568

Query: 1404 TVGYYFNTMLVVLSVYTFLWGRLYLALSGVEDA-AVASSSGNNRALGAILNQQFIIQLGL 1463
            TVG+YF+T++ VL+VY FL+GRLYL LSG+E   +      +N  L   L  Q  +Q+G 
Sbjct: 1569 TVGFYFSTLITVLTVYIFLYGRLYLVLSGLEQGLSTQKGIRDNTPLQIALASQSFVQIGF 1628

Query: 1464 FTALPMIVENSLEHGFLPAIWNFLTMQLQLASFFYTFSLGTRTHFFGRTILHGGAKYRAT 1523
              ALPM++E  LE GF  A+  F+ MQLQLA  F+TFSLGT+TH++GRT+LHGGAKYR+T
Sbjct: 1629 LMALPMLMEIGLERGFRTALSEFVLMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAKYRST 1688

Query: 1524 GRGFVVQHKSFAENYRLYARSHFVKAIELGVILIVYASRSPLARSTFTFVILSISSWFLI 1583
            GRGFVV H  FA+NYRLY+RSHFVK +E+ ++L+VY       R    +++++IS WF++
Sbjct: 1689 GRGFVVFHAKFADNYRLYSRSHFVKGLEMMLLLVVYQIFGSAYRGVLAYLLITISMWFMV 1748

Query: 1584 VSWIMAPFIFNPSGFDWLKTVYDFDDFINWLWNAGGVFTKAEQSWEAWWLEENSHLRSTG 1643
             +W+ APF+FNPSGF+W K V D+ D+  W+ N GG+   AE+SWE+WW EE  HLR +G
Sbjct: 1749 GTWLFAPFLFNPSGFEWQKIVDDWTDWNKWINNIGGIGVPAEKSWESWWEEEQEHLRYSG 1808

Query: 1644 LWGKLLEIILDLRFFFFQYAIVYHLNITGNNTSIAVYFISWVSMIVLVGIYIVIAYARDK 1703
              G ++EI+L LRFF +QY +VYHL IT    +  VY +SW+ + +++ +   ++  R +
Sbjct: 1809 KRGIVVEILLALRFFIYQYGLVYHLTITEKTKNFLVYGVSWLVIFLILFVMKTVSVGRRR 1868

Query: 1704 YAAKEHIYYRLVQLIVIVITVLVIVILMEFTPFNTGDLVTCLLAFIPTGWGIISIAQVLR 1763
            ++A   + +RL++ ++ +  + +IVIL+        D++ C+LAF+PTGWG++ IAQ  +
Sbjct: 1869 FSASFQLMFRLIKGLIFMTFIAIIVILITLAHMTIQDIIVCILAFMPTGWGMLLIAQACK 1928

Query: 1764 PFLQTTVVWDTVVSMARLYDLLFGVIAMAPLALLSWLPGFQSMQTRILFNEAFSRGLQIS 1768
            P +     W +V ++AR Y+++ G++   P+A L+W P     QTR+LFN+AFSRGLQIS
Sbjct: 1929 PVVHRAGFWGSVRTLARGYEIVMGLLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQIS 1946