HG10020312 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10020312
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Descriptionmetallothionein-like protein type 2
LocationChr04: 30788197 .. 30788847 (+)
RNA-Seq ExpressionHG10020312
SyntenyHG10020312
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGTGCTGTGGTGGAAACTGTGGGTGCGGCTCTGCCTGCAAGTGCGGAAGTGGCTGTGGAGGGTGAGTAATCGCTAATTTTTCCTTTGAATTTGATCGCGTGGAATTGCTTTTTGTTAACTTGATATATTCTGTGGAAAATGATCTCTCGATAATCTGATCTTCTGTTTTCTTGCATATCGCTTTCAATTTCTTAATCTTCTCTGCATCTTTGTTCTTCGCTCGATGGCGATCTGATCATTCGCCGATTATCTTCATCGAGTTCTGGAGTTGAGAAAGATCGCGCGATCAAGGCGGATTTTCTTCTGAAATTTCTGAAAACGACTCCGATATTCATTTTCCAGAGGATAATCGCGATGATTTTGACGTTTTGATCTTTTGTTATGCAGATGCAAGATGTTCCCTGACATGAGCTTCTCAGAGACCACCGCTACCATCGAGACCTTCGTAGTCGGTTTCGCTCCTCAGAAGATGTAAGTTTCTTCAATTACGCCTAATTTTTCAAGAGAAATTTAGGTATTTAGTCATGATTTTATTTAACGGTATCGGCGTTTTCGATATCAGGTCCTTCGAGGTAGCAGAGATGGGAGCTGAGAACGGCTGCAAGTGCGGTGACAACTGCACCTGCGATCCATGCAACTGTAAATGA

mRNA sequence

ATGTCGTGCTGTGGTGGAAACTGTGGGTGCGGCTCTGCCTGCAAGTGCGGAAGTGGCTGTGGAGGATGCAAGATGTTCCCTGACATGAGCTTCTCAGAGACCACCGCTACCATCGAGACCTTCGTAGTCGGTTTCGCTCCTCAGAAGATGTCCTTCGAGGTAGCAGAGATGGGAGCTGAGAACGGCTGCAAGTGCGGTGACAACTGCACCTGCGATCCATGCAACTGTAAATGA

Coding sequence (CDS)

ATGTCGTGCTGTGGTGGAAACTGTGGGTGCGGCTCTGCCTGCAAGTGCGGAAGTGGCTGTGGAGGATGCAAGATGTTCCCTGACATGAGCTTCTCAGAGACCACCGCTACCATCGAGACCTTCGTAGTCGGTTTCGCTCCTCAGAAGATGTCCTTCGAGGTAGCAGAGATGGGAGCTGAGAACGGCTGCAAGTGCGGTGACAACTGCACCTGCGATCCATGCAACTGTAAATGA

Protein sequence

MSCCGGNCGCGSACKCGSGCGGCKMFPDMSFSETTATIETFVVGFAPQKMSFEVAEMGAENGCKCGDNCTCDPCNCK
Homology
BLAST of HG10020312 vs. NCBI nr
Match: XP_038906044.1 (metallothionein-like protein type 2 [Benincasa hispida])

HSP 1 Score: 166.8 bits (421), Expect = 7.5e-38
Identity = 76/77 (98.70%), Postives = 76/77 (98.70%), Query Frame = 0

Query: 1  MSCCGGNCGCGSACKCGSGCGGCKMFPDMSFSETTATIETFVVGFAPQKMSFEVAEMGAE 60
          MSCCGGNCGCGS CKCGSGCGGCKMFPDMSFSETTATIETFVVGFAPQKMSFEVAEMGAE
Sbjct: 1  MSCCGGNCGCGSGCKCGSGCGGCKMFPDMSFSETTATIETFVVGFAPQKMSFEVAEMGAE 60

Query: 61 NGCKCGDNCTCDPCNCK 78
          NGCKCGDNCTCDPCNCK
Sbjct: 61 NGCKCGDNCTCDPCNCK 77

BLAST of HG10020312 vs. NCBI nr
Match: XP_022949247.1 (metallothionein-like protein type 2 [Cucurbita moschata] >XP_022971579.1 metallothionein-like protein type 2 [Cucurbita maxima] >XP_023538679.1 metallothionein-like protein type 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 164.9 bits (416), Expect = 2.8e-37
Identity = 75/77 (97.40%), Postives = 76/77 (98.70%), Query Frame = 0

Query: 1  MSCCGGNCGCGSACKCGSGCGGCKMFPDMSFSETTATIETFVVGFAPQKMSFEVAEMGAE 60
          MSCCGGNCGCGSACKCG+GCGGCKMFPDMSFSETTATIETFVVG APQKMSFEVAEMGAE
Sbjct: 1  MSCCGGNCGCGSACKCGNGCGGCKMFPDMSFSETTATIETFVVGLAPQKMSFEVAEMGAE 60

Query: 61 NGCKCGDNCTCDPCNCK 78
          NGCKCGDNCTCDPCNCK
Sbjct: 61 NGCKCGDNCTCDPCNCK 77

BLAST of HG10020312 vs. NCBI nr
Match: KAG6596409.1 (Metallothionein-like protein type 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7027952.1 Metallothionein-like protein type 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 163.3 bits (412), Expect = 8.3e-37
Identity = 74/77 (96.10%), Postives = 76/77 (98.70%), Query Frame = 0

Query: 1  MSCCGGNCGCGSACKCGSGCGGCKMFPDMSFSETTATIETFVVGFAPQKMSFEVAEMGAE 60
          MSCCGGNCGCGSACKCG+GCGGCKMFPD+SFSETTATIETFVVG APQKMSFEVAEMGAE
Sbjct: 1  MSCCGGNCGCGSACKCGNGCGGCKMFPDVSFSETTATIETFVVGLAPQKMSFEVAEMGAE 60

Query: 61 NGCKCGDNCTCDPCNCK 78
          NGCKCGDNCTCDPCNCK
Sbjct: 61 NGCKCGDNCTCDPCNCK 77

BLAST of HG10020312 vs. NCBI nr
Match: BAD26571.1 (type-2 metallothionein [Citrullus lanatus])

HSP 1 Score: 162.9 bits (411), Expect = 1.1e-36
Identity = 74/77 (96.10%), Postives = 74/77 (96.10%), Query Frame = 0

Query: 1  MSCCGGNCGCGSACKCGSGCGGCKMFPDMSFSETTATIETFVVGFAPQKMSFEVAEMGAE 60
          MSCCGGNCGCGS CKCGSGCGGCKMFPDMSFSE TATIETFVVGFAP KMSFEVAEMGAE
Sbjct: 1  MSCCGGNCGCGSGCKCGSGCGGCKMFPDMSFSEATATIETFVVGFAPHKMSFEVAEMGAE 60

Query: 61 NGCKCGDNCTCDPCNCK 78
          NGCKCGDNCTCDPCNCK
Sbjct: 61 NGCKCGDNCTCDPCNCK 77

BLAST of HG10020312 vs. NCBI nr
Match: XP_022934858.1 (metallothionein-like protein type 2 B [Cucurbita moschata] >XP_023529118.1 metallothionein-like protein type 2 B [Cucurbita pepo subsp. pepo] >KAG6580545.1 Metallothionein-like protein type 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7017297.1 Metallothionein-like protein type 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 162.2 bits (409), Expect = 1.8e-36
Identity = 73/77 (94.81%), Postives = 75/77 (97.40%), Query Frame = 0

Query: 1  MSCCGGNCGCGSACKCGSGCGGCKMFPDMSFSETTATIETFVVGFAPQKMSFEVAEMGAE 60
          MSCCGGNCGCGSACKCG+GCGGCKMFPDMSFSETTATIETFVVG APQKMSFE +EMGAE
Sbjct: 1  MSCCGGNCGCGSACKCGNGCGGCKMFPDMSFSETTATIETFVVGLAPQKMSFEASEMGAE 60

Query: 61 NGCKCGDNCTCDPCNCK 78
          NGCKCGDNCTCDPCNCK
Sbjct: 61 NGCKCGDNCTCDPCNCK 77

BLAST of HG10020312 vs. ExPASy Swiss-Prot
Match: Q40158 (Metallothionein-like protein type 2 B OS=Solanum lycopersicum OX=4081 GN=MTB PE=2 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 1.4e-26
Identity = 53/80 (66.25%), Postives = 64/80 (80.00%), Query Frame = 0

Query: 1  MSCCGGNCGCGSACKCGSGCGGCKMFPDMSFSETTATIETFVVGFAPQKMSFEVAEMG-- 60
          MSCCGGNCGCGS+CKCG+GCGGCKM+PDMS++E++ T ET V+G  P+K SF   EMG  
Sbjct: 1  MSCCGGNCGCGSSCKCGNGCGGCKMYPDMSYTESSTTTETLVLGVGPEKTSFGAMEMGES 60

Query: 61 --AENGCKCGDNCTCDPCNC 77
            AENGCKCG +C C+PC C
Sbjct: 61 PVAENGCKCGSDCKCNPCTC 80

BLAST of HG10020312 vs. ExPASy Swiss-Prot
Match: P30564 (Metallothionein-like protein type 2 OS=Ricinus communis OX=3988 GN=MTI PE=3 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 1.4e-26
Identity = 57/81 (70.37%), Postives = 62/81 (76.54%), Query Frame = 0

Query: 1  MSCCGGNCGCGSACKCGSGCGGCKMFPDMSFSETTATIETFVVGFAPQKMSFEVAEMGA- 60
          MSCCGGNCGCGS CKCG+GCGGCKM+PDMSFSE T T ET V+G   +K  FE  EMG  
Sbjct: 1  MSCCGGNCGCGSGCKCGNGCGGCKMYPDMSFSEKTTT-ETLVLGVGAEKAHFEGGEMGVV 60

Query: 61 ---ENGCKCGDNCTCDPCNCK 78
             E GCKCGDNCTC+PC CK
Sbjct: 61 GAEEGGCKCGDNCTCNPCTCK 80

BLAST of HG10020312 vs. ExPASy Swiss-Prot
Match: P43390 (Metallothionein-like protein type 2 OS=Actinidia deliciosa OX=3627 GN=pKIWI504 PE=2 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 3.1e-26
Identity = 56/79 (70.89%), Postives = 63/79 (79.75%), Query Frame = 0

Query: 1  MSCCGGNCGCGSACKCGSGCGGCKMFPDMSFSETTATIETFVVGFAPQKMSFEVAEMG-- 60
          MSCCGG CGCGS+C CGSGCGGC M+PD+S+SE T T ET +VG APQK  FE +EMG  
Sbjct: 1  MSCCGGKCGCGSSCSCGSGCGGCGMYPDLSYSEMTTT-ETLIVGVAPQKTYFEGSEMGVA 60

Query: 61 AENGCKCGDNCTCDPCNCK 78
          AENGCKCG +C CDPC CK
Sbjct: 61 AENGCKCGSDCKCDPCTCK 78

BLAST of HG10020312 vs. ExPASy Swiss-Prot
Match: Q39459 (Metallothionein-like protein 2 OS=Cicer arietinum OX=3827 PE=3 SV=2)

HSP 1 Score: 118.2 bits (295), Expect = 4.0e-26
Identity = 59/80 (73.75%), Postives = 65/80 (81.25%), Query Frame = 0

Query: 1  MSCCGGNCGCGSACKCGSGCGGCKMFPDMSFSETTATIETFVVGFAPQKMSFEVAEM--G 60
          MSCCGGNCGCGS+CKCGSGCGGCKM+PDMS++E T T ET V+G A  K  FE AEM  G
Sbjct: 1  MSCCGGNCGCGSSCKCGSGCGGCKMYPDMSYTEQT-TSETLVMGVASGKTQFEGAEMGFG 60

Query: 61 AEN-GCKCGDNCTCDPCNCK 78
          AEN GCKCG NCTC+PC CK
Sbjct: 61 AENDGCKCGSNCTCNPCTCK 79

BLAST of HG10020312 vs. ExPASy Swiss-Prot
Match: Q41657 (Metallothionein-like protein type 2 OS=Vicia faba OX=3906 GN=MTI PE=2 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 5.2e-26
Identity = 54/78 (69.23%), Postives = 65/78 (83.33%), Query Frame = 0

Query: 1  MSCCGGNCGCGSACKCGSGCGGCKMFPDMSFSETTATIETFVVGFAPQKMSFEVAEMGAE 60
          MSCCGGNCGCGS+CKCGSGCGGCKM+ D+S++E+T T ET ++G   +K  +E AEMGAE
Sbjct: 1  MSCCGGNCGCGSSCKCGSGCGGCKMYADLSYTEST-TSETLIMGVGSEKAQYESAEMGAE 60

Query: 61 N-GCKCGDNCTCDPCNCK 78
          N GCKCG NCTC+PC CK
Sbjct: 61 NDGCKCGANCTCNPCTCK 77

BLAST of HG10020312 vs. ExPASy TrEMBL
Match: A0A6J1I8Z4 (metallothionein-like protein type 2 OS=Cucurbita maxima OX=3661 GN=LOC111470252 PE=3 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 1.4e-37
Identity = 75/77 (97.40%), Postives = 76/77 (98.70%), Query Frame = 0

Query: 1  MSCCGGNCGCGSACKCGSGCGGCKMFPDMSFSETTATIETFVVGFAPQKMSFEVAEMGAE 60
          MSCCGGNCGCGSACKCG+GCGGCKMFPDMSFSETTATIETFVVG APQKMSFEVAEMGAE
Sbjct: 1  MSCCGGNCGCGSACKCGNGCGGCKMFPDMSFSETTATIETFVVGLAPQKMSFEVAEMGAE 60

Query: 61 NGCKCGDNCTCDPCNCK 78
          NGCKCGDNCTCDPCNCK
Sbjct: 61 NGCKCGDNCTCDPCNCK 77

BLAST of HG10020312 vs. ExPASy TrEMBL
Match: A0A6J1GBI9 (metallothionein-like protein type 2 OS=Cucurbita moschata OX=3662 GN=LOC111452655 PE=3 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 1.4e-37
Identity = 75/77 (97.40%), Postives = 76/77 (98.70%), Query Frame = 0

Query: 1  MSCCGGNCGCGSACKCGSGCGGCKMFPDMSFSETTATIETFVVGFAPQKMSFEVAEMGAE 60
          MSCCGGNCGCGSACKCG+GCGGCKMFPDMSFSETTATIETFVVG APQKMSFEVAEMGAE
Sbjct: 1  MSCCGGNCGCGSACKCGNGCGGCKMFPDMSFSETTATIETFVVGLAPQKMSFEVAEMGAE 60

Query: 61 NGCKCGDNCTCDPCNCK 78
          NGCKCGDNCTCDPCNCK
Sbjct: 61 NGCKCGDNCTCDPCNCK 77

BLAST of HG10020312 vs. ExPASy TrEMBL
Match: Q6I674 (Type-2 metallothionein OS=Citrullus lanatus OX=3654 GN=CLMT2 PE=2 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 5.2e-37
Identity = 74/77 (96.10%), Postives = 74/77 (96.10%), Query Frame = 0

Query: 1  MSCCGGNCGCGSACKCGSGCGGCKMFPDMSFSETTATIETFVVGFAPQKMSFEVAEMGAE 60
          MSCCGGNCGCGS CKCGSGCGGCKMFPDMSFSE TATIETFVVGFAP KMSFEVAEMGAE
Sbjct: 1  MSCCGGNCGCGSGCKCGSGCGGCKMFPDMSFSEATATIETFVVGFAPHKMSFEVAEMGAE 60

Query: 61 NGCKCGDNCTCDPCNCK 78
          NGCKCGDNCTCDPCNCK
Sbjct: 61 NGCKCGDNCTCDPCNCK 77

BLAST of HG10020312 vs. ExPASy TrEMBL
Match: A0A6J1F8X2 (metallothionein-like protein type 2 B OS=Cucurbita moschata OX=3662 GN=LOC111441898 PE=3 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 8.9e-37
Identity = 73/77 (94.81%), Postives = 75/77 (97.40%), Query Frame = 0

Query: 1  MSCCGGNCGCGSACKCGSGCGGCKMFPDMSFSETTATIETFVVGFAPQKMSFEVAEMGAE 60
          MSCCGGNCGCGSACKCG+GCGGCKMFPDMSFSETTATIETFVVG APQKMSFE +EMGAE
Sbjct: 1  MSCCGGNCGCGSACKCGNGCGGCKMFPDMSFSETTATIETFVVGLAPQKMSFEASEMGAE 60

Query: 61 NGCKCGDNCTCDPCNCK 78
          NGCKCGDNCTCDPCNCK
Sbjct: 61 NGCKCGDNCTCDPCNCK 77

BLAST of HG10020312 vs. ExPASy TrEMBL
Match: A0A1S3B6L7 (metallothionein-like protein type 2 OS=Cucumis melo OX=3656 GN=LOC103486389 PE=3 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 3.4e-36
Identity = 72/77 (93.51%), Postives = 75/77 (97.40%), Query Frame = 0

Query: 1  MSCCGGNCGCGSACKCGSGCGGCKMFPDMSFSETTATIETFVVGFAPQKMSFEVAEMGAE 60
          MSCCGGNCGCGSACKCG+GCGGCK FPD+SFSETTATIETFVVGFAPQKMS+EVAEMGAE
Sbjct: 1  MSCCGGNCGCGSACKCGNGCGGCKSFPDLSFSETTATIETFVVGFAPQKMSYEVAEMGAE 60

Query: 61 NGCKCGDNCTCDPCNCK 78
          NGCKCGDNCTCDPC CK
Sbjct: 61 NGCKCGDNCTCDPCTCK 77

BLAST of HG10020312 vs. TAIR 10
Match: AT3G09390.1 (metallothionein 2A )

HSP 1 Score: 107.1 bits (266), Expect = 6.5e-24
Identity = 52/81 (64.20%), Postives = 59/81 (72.84%), Query Frame = 0

Query: 1  MSCCGGNCGCGSACKCGSGCGGCKMFPDMSFSETTATIETFVVGFAP-QKMSFEVA--EM 60
          MSCCGGNCGCGS CKCG+GCGGCKM+PD+ FS  T T ETFV+G AP  K  +E +    
Sbjct: 1  MSCCGGNCGCGSGCKCGNGCGGCKMYPDLGFSGETTTTETFVLGVAPAMKNQYEASGESN 60

Query: 61 GAEN-GCKCGDNCTCDPCNCK 78
           AEN  CKCG +C CDPC CK
Sbjct: 61 NAENDACKCGSDCKCDPCTCK 81

BLAST of HG10020312 vs. TAIR 10
Match: AT5G02380.1 (metallothionein 2B )

HSP 1 Score: 89.4 bits (220), Expect = 1.4e-18
Identity = 46/81 (56.79%), Postives = 55/81 (67.90%), Query Frame = 0

Query: 1  MSCCGGNCGCGSACKCGSGCGGCKMFPDMSFSETTATIETFVVGFAPQKMSFEVAE---- 60
          MSCCGG+CGCGSACKCG+GCGGCK +PD+   E TAT ET V+G AP   S   A     
Sbjct: 1  MSCCGGSCGCGSACKCGNGCGGCKRYPDL---ENTAT-ETLVLGVAPAMNSQYEASGETF 60

Query: 61 MGAENGCKCGDNCTCDPCNCK 78
          +   + CKCG +C C+PC CK
Sbjct: 61 VAENDACKCGSDCKCNPCTCK 77

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038906044.17.5e-3898.70metallothionein-like protein type 2 [Benincasa hispida][more]
XP_022949247.12.8e-3797.40metallothionein-like protein type 2 [Cucurbita moschata] >XP_022971579.1 metallo... [more]
KAG6596409.18.3e-3796.10Metallothionein-like protein type 2, partial [Cucurbita argyrosperma subsp. soro... [more]
BAD26571.11.1e-3696.10type-2 metallothionein [Citrullus lanatus][more]
XP_022934858.11.8e-3694.81metallothionein-like protein type 2 B [Cucurbita moschata] >XP_023529118.1 metal... [more]
Match NameE-valueIdentityDescription
Q401581.4e-2666.25Metallothionein-like protein type 2 B OS=Solanum lycopersicum OX=4081 GN=MTB PE=... [more]
P305641.4e-2670.37Metallothionein-like protein type 2 OS=Ricinus communis OX=3988 GN=MTI PE=3 SV=1[more]
P433903.1e-2670.89Metallothionein-like protein type 2 OS=Actinidia deliciosa OX=3627 GN=pKIWI504 P... [more]
Q394594.0e-2673.75Metallothionein-like protein 2 OS=Cicer arietinum OX=3827 PE=3 SV=2[more]
Q416575.2e-2669.23Metallothionein-like protein type 2 OS=Vicia faba OX=3906 GN=MTI PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1I8Z41.4e-3797.40metallothionein-like protein type 2 OS=Cucurbita maxima OX=3661 GN=LOC111470252 ... [more]
A0A6J1GBI91.4e-3797.40metallothionein-like protein type 2 OS=Cucurbita moschata OX=3662 GN=LOC11145265... [more]
Q6I6745.2e-3796.10Type-2 metallothionein OS=Citrullus lanatus OX=3654 GN=CLMT2 PE=2 SV=1[more]
A0A6J1F8X28.9e-3794.81metallothionein-like protein type 2 B OS=Cucurbita moschata OX=3662 GN=LOC111441... [more]
A0A1S3B6L73.4e-3693.51metallothionein-like protein type 2 OS=Cucumis melo OX=3656 GN=LOC103486389 PE=3... [more]
Match NameE-valueIdentityDescription
AT3G09390.16.5e-2464.20metallothionein 2A [more]
AT5G02380.11.4e-1856.79metallothionein 2B [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000347Metallothionein, family 15, plantPFAMPF01439Metallothio_2coord: 1..76
e-value: 2.4E-29
score: 102.1
IPR000347Metallothionein, family 15, plantPANTHERPTHR33543METALLOTHIONEIN-LIKE PROTEIN 2Acoord: 1..76
NoneNo IPR availablePANTHERPTHR33543:SF1METALLOTHIONEIN-LIKE PROTEIN 2Acoord: 1..76

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10020312.1HG10020312.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0046872 metal ion binding