HG10020086 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10020086
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionIAA-amino acid hydrolase ILR1-like 3
LocationChr04: 28640278 .. 28643889 (-)
RNA-Seq ExpressionHG10020086
SyntenyHG10020086
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAAAATTACTTCTATGCGCGTTCTTTTTCATCTTCCAATTCTGTATATCATCGCCCCTGGAAACTGAACCGCCATTGGAGTTGAGTCGTCTAACTCGGGAGCTTTTGGAATCGGCGAGAAACCCGGAGTTCTTCGAGTGGTTGGTTAGGGCTCGAAGGAAATTGCACGAGAATCCAGAGCTGTCCTTCGAGGAATTCGAAACCAGCCAATTCATCCGAACAGAGCTGGAGTCTCTTGGGATTAATTTCACTTGGCCCGTCGCTAAGACCGGGATTGTCGCCTCCGTCGGATCAGGCACGCACCCATGGTTCGCTCTTAGGGCTGATATGGATGCTCTTCCTATTCAGGTTTCCTTTCTTATCCCTTCACATACAATTCCCTTTGTCTGTTTCGATATTCTTATCACTCTTTTCATTGTTGATTTGTTTTGAGATAAAATATATTCGGAAGGATCGGTGCATGTGATTTTGAAATTATTATAATTACTTTTTTCATAGAATCACTTCGAAAAATACTTTTAATTATTTAGTATCAATTTATCGTTTAATCTCACACTTTTAAGTGCATTTTTTATAGTATTAAATTGAATTTTAAATAATATTTTTTTGAGTGATTTTAAAAATGACAAAATGAATTTTAACCATTTTAGAATCACTTCTAAACATGCAACTAAAATGATTGTGTGTTTCTATAAATTTAACTCTTTAAGTGATGGCATGTTGAAAGGAAAAGGTTGAGTGGGAACATAAGAGTAAAAAGGATGGTAAAATGCATGCTTGTGGCCATGATGTTCATGTCACAATGCTTCTTGGAGCTGCTAAGTTGCTTCAGCAAAGAAGAAATGAGCTAAAGGTAAATTTACTAGTATTTACGTCAACAAAGGGTTGGGTTTTGTTCTAATGCAATACGGTAGGGTCATTGTCTATGCTTCTTGTTAATTCACTTTGGATACCTAATGCGATTATGAGTATAATCTAATAAACGTGTATGCTCTTCTACACGTTGGACACTACCTTCAACCGAGTTTTGTACGAGGTTAGTGGAGCCAAAGACATGGAGTGGGATCAACAATGGTGACATGATTGTGTCGAGAGACTAACGTGCTACATGCGTGTGTCTCCTCGGTCGAGAAACTTGTTGAGCCATACTAGGATGCAAACTTGGACAACACTTGACTATTCACACTCTTAATAGTATAAAATTTAAAAGTAAAAAAAATGAGAGAAAACAAGTATAAATTTAAAAAATGGAAAATGGAAAACGAAAAACAAATTGTTATTAAATGAGAGTTTTGATTGTTAAGTTTGTAAAGTGTGTTATGTGAAATGTGAAAGGGGACAGTGAAGCTTGTTTTCCAACCAGGGGAAGAGGGACGTGGTGGGGCTTACTATATGGTAAAAGAAGGTTGTGTGGAGAAGGTGGAAGGCATATTTGGATTACATGTCGCACAAGATATGTCAGTAGGGGCAATTGGTTCAAGACCAGGTCCATTTACGGCTTGTAGTGGTAGATTTTTGGCTACAATCCAAGGAATTGGTGGTCATGCTGCACTGCCTCATAAAGCAAGAGATCCAGTTCTTGCCATGTCATCGGCTATTATCTCTCTTCAGTACATAATTTCTCGAGAAACAGATCCTCTTGATTCCAGGGTAATCTTATTTTCCCCAACATAACTATGGAAAATATTCTTCAAACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCTTCAATATTCTTTTCGAGTTGAACTTTTTTTTTTGTTTTTTTGAAATAAATTTACATTTTTTTTTATAAGTTTTGATTATAGCTTCATTTAGTTTATAAGTTTCAAATTAAAATATTACCTTTTTATCCTAATATTTTTATTTTGTTTTTAGATATTTCAAATTCTTACATTTTTACTTTTAAATTTGTTAATTAAATTTCATTTAATAGTGATAGGAAATTAATGAAAATTAATTTAATTGTTCAAAATAAATAATTAAAGAAAATTAATACTAAGGATTGAAAGTTGGACACATTAATAAAAATTCAAGAGGAAAATTGTAAAATCTTCCGACGTTTTGGACTAAATGAAACTAAGTATAATTTTAGAACTATATTTTGTGAGTGATTTTAAATTAGATGACACAAGTGATTCCAACCATTTTAAAATTATCTTCAAACATTTTTTAAGATTTTAAATTTATTCATATTCATGAGGTGTCATATTGTTGATTATATATATATTCATTATTGGTAGGTGATTACGGTAGGATTAGTTAAGGGTGGCGAAACAGGAAATGTGATACCCGAAACCGTAACATTTGGAGGTACTTTCCGAAGCATCACTTTGGAAGGTCTTTACAACCTTAAGCAAAGAATTCAAGAGGTAACGAACTCCCAAACTTCACCTCCTACATTGTGAGAATATTTTTATCCACATTGAAAATTAAATAAGGAAAAAGAAACATTCAGTTTAGGATTTGTCGATTTAAGCATGACTCAATTTAGTTTTGAATTATAGCTTCCATTTGATAACGATTTTTCTCTTAATTTTTTTAATTAAGGTTTTCACGTTTGTTAACAAAAGACATGAATTCTTAGTTCAGTCAAATTCTAAGAATAAAAACAAGTATTTTTTTTAACCGTTTTCAAAACATAGCTTGGTCGTTTTTTAAAACATTAGTAAAAATTGGATAACAAAACACAAAATTAACTTAAGGTAAGAAACTAGTGTTTTAAACAAGACCAACACTTTTGGTCTCCGAGGTTTGAGTTCGGTAACATCTGAGTCGTTACTAGATAATGAGTTTCAAACTTTACATCCTAGCTACATTTTTGAGTTTGATAGCATTAATAGTCCTGCCTTTTAGTTTTTCAGTAATTACTGGTAAGAATGTTGGCATATGACACTATTTTTATTAGAAAATTAGAATAAAAAGAAGGAGAAGGTTGGAAGGAAGGAGAAGAAGATAGAAAAAGAGAATGAGAACAAAAAAAAAAAAAAAAAATCAAAATATACATTTAACAAAAAAACTAACACGTGACGAAAATGCTACCAAACTCAAATTTCATGGACTAAAATCTCGAGTTTGAAAATTCAAAGACCAAAATTTACTACAAAACTCAAAGCTCAAAGAAGGAAAAACATAATTTTGTAGAAAAGAAATGGTGTTTAGGATTTATGATACATAAGCAACTATGTGTTGGGTTGAATGATCCCAACAGGTAATAGAGTTTCAAGTAGGTGTGCATCAGTGTAATGCCACTGTAGACTTCTTGGAAGAAAAGGCAAGGTTCTACCCACCTACTATCAATGATCAATCATTATACAACCACATTGAGAAGGTTGGACAACATTTGCTTGGAGGTTCATCAAATGTTCTTCATCTTCCCAACACCATGGGAGCAGAGGACTTCAGCTTCTACTCACAACACATTCCTGCTGCATTTTTCATGATCGGAGCCAAGAACGATACCATGGAATCCGGAATACCGTTGCACTCGCCGTACCTTGTCCTAGATGAACAAGTTCTTCCACTTGGAGCTGCTCTTCATGCTGCAGTTGCAATTTCTTACTTGGATCAACATTATTACTCTGTGCCCAGCAATTAA

mRNA sequence

ATGGAAAAATTACTTCTATGCGCGTTCTTTTTCATCTTCCAATTCTGTATATCATCGCCCCTGGAAACTGAACCGCCATTGGAGTTGAGTCGTCTAACTCGGGAGCTTTTGGAATCGGCGAGAAACCCGGAGTTCTTCGAGTGGTTGGTTAGGGCTCGAAGGAAATTGCACGAGAATCCAGAGCTGTCCTTCGAGGAATTCGAAACCAGCCAATTCATCCGAACAGAGCTGGAGTCTCTTGGGATTAATTTCACTTGGCCCGTCGCTAAGACCGGGATTGTCGCCTCCGTCGGATCAGGCACGCACCCATGGTTCGCTCTTAGGGCTGATATGGATGCTCTTCCTATTCAGGAAAAGGTTGAGTGGGAACATAAGAGTAAAAAGGATGGTAAAATGCATGCTTGTGGCCATGATGTTCATGTCACAATGCTTCTTGGAGCTGCTAAGTTGCTTCAGCAAAGAAGAAATGAGCTAAAGGGGACAGTGAAGCTTGTTTTCCAACCAGGGGAAGAGGGACGTGGTGGGGCTTACTATATGGTAAAAGAAGGTTGTGTGGAGAAGGTGGAAGGCATATTTGGATTACATGTCGCACAAGATATGTCAGTAGGGGCAATTGGTTCAAGACCAGGTCCATTTACGGCTTGTAGTGGTAGATTTTTGGCTACAATCCAAGGAATTGGTGGTCATGCTGCACTGCCTCATAAAGCAAGAGATCCAGTTCTTGCCATGTCATCGGCTATTATCTCTCTTCAGTACATAATTTCTCGAGAAACAGATCCTCTTGATTCCAGGGTGATTACGGTAGGATTAGTTAAGGGTGGCGAAACAGGAAATGTGATACCCGAAACCGTAACATTTGGAGGTACTTTCCGAAGCATCACTTTGGAAGGTCTTTACAACCTTAAGCAAAGAATTCAAGAGGTAATAGAGTTTCAAGTAGGTGTGCATCAGTGTAATGCCACTGTAGACTTCTTGGAAGAAAAGGCAAGGTTCTACCCACCTACTATCAATGATCAATCATTATACAACCACATTGAGAAGGTTGGACAACATTTGCTTGGAGGTTCATCAAATGTTCTTCATCTTCCCAACACCATGGGAGCAGAGGACTTCAGCTTCTACTCACAACACATTCCTGCTGCATTTTTCATGATCGGAGCCAAGAACGATACCATGGAATCCGGAATACCGTTGCACTCGCCGTACCTTGTCCTAGATGAACAAGTTCTTCCACTTGGAGCTGCTCTTCATGCTGCAGTTGCAATTTCTTACTTGGATCAACATTATTACTCTGTGCCCAGCAATTAA

Coding sequence (CDS)

ATGGAAAAATTACTTCTATGCGCGTTCTTTTTCATCTTCCAATTCTGTATATCATCGCCCCTGGAAACTGAACCGCCATTGGAGTTGAGTCGTCTAACTCGGGAGCTTTTGGAATCGGCGAGAAACCCGGAGTTCTTCGAGTGGTTGGTTAGGGCTCGAAGGAAATTGCACGAGAATCCAGAGCTGTCCTTCGAGGAATTCGAAACCAGCCAATTCATCCGAACAGAGCTGGAGTCTCTTGGGATTAATTTCACTTGGCCCGTCGCTAAGACCGGGATTGTCGCCTCCGTCGGATCAGGCACGCACCCATGGTTCGCTCTTAGGGCTGATATGGATGCTCTTCCTATTCAGGAAAAGGTTGAGTGGGAACATAAGAGTAAAAAGGATGGTAAAATGCATGCTTGTGGCCATGATGTTCATGTCACAATGCTTCTTGGAGCTGCTAAGTTGCTTCAGCAAAGAAGAAATGAGCTAAAGGGGACAGTGAAGCTTGTTTTCCAACCAGGGGAAGAGGGACGTGGTGGGGCTTACTATATGGTAAAAGAAGGTTGTGTGGAGAAGGTGGAAGGCATATTTGGATTACATGTCGCACAAGATATGTCAGTAGGGGCAATTGGTTCAAGACCAGGTCCATTTACGGCTTGTAGTGGTAGATTTTTGGCTACAATCCAAGGAATTGGTGGTCATGCTGCACTGCCTCATAAAGCAAGAGATCCAGTTCTTGCCATGTCATCGGCTATTATCTCTCTTCAGTACATAATTTCTCGAGAAACAGATCCTCTTGATTCCAGGGTGATTACGGTAGGATTAGTTAAGGGTGGCGAAACAGGAAATGTGATACCCGAAACCGTAACATTTGGAGGTACTTTCCGAAGCATCACTTTGGAAGGTCTTTACAACCTTAAGCAAAGAATTCAAGAGGTAATAGAGTTTCAAGTAGGTGTGCATCAGTGTAATGCCACTGTAGACTTCTTGGAAGAAAAGGCAAGGTTCTACCCACCTACTATCAATGATCAATCATTATACAACCACATTGAGAAGGTTGGACAACATTTGCTTGGAGGTTCATCAAATGTTCTTCATCTTCCCAACACCATGGGAGCAGAGGACTTCAGCTTCTACTCACAACACATTCCTGCTGCATTTTTCATGATCGGAGCCAAGAACGATACCATGGAATCCGGAATACCGTTGCACTCGCCGTACCTTGTCCTAGATGAACAAGTTCTTCCACTTGGAGCTGCTCTTCATGCTGCAGTTGCAATTTCTTACTTGGATCAACATTATTACTCTGTGCCCAGCAATTAA

Protein sequence

MEKLLLCAFFFIFQFCISSPLETEPPLELSRLTRELLESARNPEFFEWLVRARRKLHENPELSFEEFETSQFIRTELESLGINFTWPVAKTGIVASVGSGTHPWFALRADMDALPIQEKVEWEHKSKKDGKMHACGHDVHVTMLLGAAKLLQQRRNELKGTVKLVFQPGEEGRGGAYYMVKEGCVEKVEGIFGLHVAQDMSVGAIGSRPGPFTACSGRFLATIQGIGGHAALPHKARDPVLAMSSAIISLQYIISRETDPLDSRVITVGLVKGGETGNVIPETVTFGGTFRSITLEGLYNLKQRIQEVIEFQVGVHQCNATVDFLEEKARFYPPTINDQSLYNHIEKVGQHLLGGSSNVLHLPNTMGAEDFSFYSQHIPAAFFMIGAKNDTMESGIPLHSPYLVLDEQVLPLGAALHAAVAISYLDQHYYSVPSN
Homology
BLAST of HG10020086 vs. NCBI nr
Match: XP_004136497.2 (IAA-amino acid hydrolase ILR1-like 3 [Cucumis sativus] >KAE8651096.1 hypothetical protein Csa_001046 [Cucumis sativus])

HSP 1 Score: 780.4 bits (2014), Expect = 8.1e-222
Identity = 381/435 (87.59%), Postives = 409/435 (94.02%), Query Frame = 0

Query: 1   MEKLLLCAFFFIFQFCISSPLETEPPLELSRLTRELLESARNPEFFEWLVRARRKLHENP 60
           ME++++   FFIF FCISS +E EPPLELSRLTRELLESAR+PEFFEWLV+ARRKLHENP
Sbjct: 59  MEQIIVWGLFFIFPFCISSGMEAEPPLELSRLTRELLESARDPEFFEWLVKARRKLHENP 118

Query: 61  ELSFEEFETSQFIRTELESLGINFTWPVAKTGIVASVGSGTHPWFALRADMDALPIQEKV 120
           EL+FEEFETS+FIRTELES+GINF WP+AKTGIVASVGSG HPWFALRADMDALPIQE V
Sbjct: 119 ELAFEEFETSEFIRTELESVGINFNWPLAKTGIVASVGSGAHPWFALRADMDALPIQEMV 178

Query: 121 EWEHKSKKDGKMHACGHDVHVTMLLGAAKLLQQRRNELKGTVKLVFQPGEEGRGGAYYMV 180
           EWEHKSKKDGKMHACGHDVHVTMLLGAAKLLQQRRNELKGTVKLVFQPGEEGRGGAYYMV
Sbjct: 179 EWEHKSKKDGKMHACGHDVHVTMLLGAAKLLQQRRNELKGTVKLVFQPGEEGRGGAYYMV 238

Query: 181 KEGCVEKVEGIFGLHVAQDMSVGAIGSRPGPFTACSGRFLATIQGIGGHAALPHKARDPV 240
           KEG +E V+GIFGLHVAQDM++GAIGSRPGPFTACSGRFLATIQGIGGHAALPH+A+DP+
Sbjct: 239 KEGAIENVKGIFGLHVAQDMTLGAIGSRPGPFTACSGRFLATIQGIGGHAALPHQAKDPL 298

Query: 241 LAMSSAIISLQYIISRETDPLDSRVITVGLVKGGETGNVIPETVTFGGTFRSITLEGLYN 300
           LAMSSAIISLQ+IISRETDP DSRVI+VGLVKGGE  NVIPETVTFGGTFRS TLEGLYN
Sbjct: 299 LAMSSAIISLQHIISRETDPFDSRVISVGLVKGGEARNVIPETVTFGGTFRSKTLEGLYN 358

Query: 301 LKQRIQEVIEFQVGVHQCNATVDFLEEKARFYPPTINDQSLYNHIEKVGQHLLGGSSNVL 360
           LK RIQ+VIEFQV V+ C+A VDF+EEKARFYPPTINDQSLY+H+  VGQHLLGG SNVL
Sbjct: 359 LKHRIQQVIEFQVAVYGCSAIVDFMEEKARFYPPTINDQSLYDHVNNVGQHLLGGPSNVL 418

Query: 361 HLPNTMGAEDFSFYSQHIPAAFFMIGAKNDTMESGIPLHSPYLVLDEQVLPLGAALHAAV 420
           HLPNTMGAEDFSFYSQHIPAAFFMIGAKNDTMESGIPLHSPYLVLDE VLPLGAALHAAV
Sbjct: 419 HLPNTMGAEDFSFYSQHIPAAFFMIGAKNDTMESGIPLHSPYLVLDEHVLPLGAALHAAV 478

Query: 421 AISYLDQHYYSVPSN 436
           AISYLDQ ++ V SN
Sbjct: 479 AISYLDQQHHFVSSN 493

BLAST of HG10020086 vs. NCBI nr
Match: XP_038906100.1 (IAA-amino acid hydrolase ILR1-like 3 [Benincasa hispida])

HSP 1 Score: 761.9 bits (1966), Expect = 3.0e-216
Identity = 386/436 (88.53%), Postives = 402/436 (92.20%), Query Frame = 0

Query: 1   MEKLLLCAFFFIFQFCISSPLETEPPLELSRLTRELLESARNPEFFEWLVRARRKLHENP 60
           M  LLL AFF IF FCI+S LETEPPLELSRLTRELLESAR PEFFEWLVRARRKLHENP
Sbjct: 1   MGLLLLWAFFSIFPFCITSTLETEPPLELSRLTRELLESARKPEFFEWLVRARRKLHENP 60

Query: 61  ELSFEEFETSQFIRTELESLGINFTWPVAKTGIVASVGSGTHPWFALRADMDALPIQEKV 120
           ELSFEEFETSQFIRTEL SLGINFTWPVAKTGIVASVGSG HPWFALRADMDALPIQE V
Sbjct: 61  ELSFEEFETSQFIRTELVSLGINFTWPVAKTGIVASVGSGGHPWFALRADMDALPIQEMV 120

Query: 121 EWEHKSKKDGKMHACGHDVHVTMLLGAAKLLQQRRNELKGTVKLVFQPGEEGRGGAYYMV 180
           EWEHKSK DGKMHACGHDVHVTMLLGAAKLL QRRNELKGTVKLVFQPGEEGRGGAYYMV
Sbjct: 121 EWEHKSKNDGKMHACGHDVHVTMLLGAAKLLHQRRNELKGTVKLVFQPGEEGRGGAYYMV 180

Query: 181 KEGCVEKVEGIFGLHVAQDMSVGAIGSRPGPFTACSGRFLATIQGIGGHAALPHKARDPV 240
           KEG VE+V+GIFGLHVAQDM+VG IGSRPGPFTACSGRFLATIQGIGG      +A D +
Sbjct: 181 KEGAVEEVKGIFGLHVAQDMTVGVIGSRPGPFTACSGRFLATIQGIGG-----RQATDSL 240

Query: 241 LAMSSAIISLQYIISRETDPLDSRVITVGLVKGGETGNV-IPETVTFGGTFRSITLEGLY 300
           LAMSSAIISLQ+IISRETDPLDSRVI+VG VKGGE GNV IPETVTFGGTFRSI LEGLY
Sbjct: 241 LAMSSAIISLQHIISRETDPLDSRVISVGFVKGGEAGNVIIPETVTFGGTFRSIALEGLY 300

Query: 301 NLKQRIQEVIEFQVGVHQCNATVDFLEEKARFYPPTINDQSLYNHIEKVGQHLLGGSSNV 360
           NLKQRIQEVI+ Q  VHQC ATVDF+EEKARFYPP INDQSLYNH++KVGQHLLGG SNV
Sbjct: 301 NLKQRIQEVIKLQSAVHQCRATVDFMEEKARFYPPIINDQSLYNHVKKVGQHLLGGPSNV 360

Query: 361 LHLPNTMGAEDFSFYSQHIPAAFFMIGAKNDTMESGIPLHSPYLVLDEQVLPLGAALHAA 420
           LHLPNTMGAEDFSFYSQHIPAAFFMIGAKN TM+SGIPLHSPYLVLDEQVLPLGAALHAA
Sbjct: 361 LHLPNTMGAEDFSFYSQHIPAAFFMIGAKNITMDSGIPLHSPYLVLDEQVLPLGAALHAA 420

Query: 421 VAISYLDQHYYSVPSN 436
           VAISYLDQH++ VP+N
Sbjct: 421 VAISYLDQHHH-VPNN 430

BLAST of HG10020086 vs. NCBI nr
Match: XP_008442881.1 (PREDICTED: IAA-amino acid hydrolase ILR1-like 3 [Cucumis melo])

HSP 1 Score: 751.9 bits (1940), Expect = 3.1e-213
Identity = 368/435 (84.60%), Postives = 400/435 (91.95%), Query Frame = 0

Query: 1   MEKLLLCAFFFIFQFCISSPLETEPPLELSRLTRELLESARNPEFFEWLVRARRKLHENP 60
           ME +++   FFI  FCISS ++ EPPLELSRLTR+LLESAR+PEFFEWLVR RRKLHENP
Sbjct: 1   MEIIIVWGLFFILPFCISSGMKAEPPLELSRLTRDLLESARDPEFFEWLVRTRRKLHENP 60

Query: 61  ELSFEEFETSQFIRTELESLGINFTWPVAKTGIVASVGSGTHPWFALRADMDALPIQEKV 120
           EL+FEEFETS+FIRTELES+GINF WPVAKTGIVASVGSG HPWFALRADMDALPIQE V
Sbjct: 61  ELAFEEFETSEFIRTELESVGINFNWPVAKTGIVASVGSGAHPWFALRADMDALPIQEMV 120

Query: 121 EWEHKSKKDGKMHACGHDVHVTMLLGAAKLLQQRRNELKGTVKLVFQPGEEGRGGAYYMV 180
           EWEHKSKKDGKMHACGHDVHVTMLLGAAKLLQQRRNELKGTVKLVFQPGEEGRGGAY+MV
Sbjct: 121 EWEHKSKKDGKMHACGHDVHVTMLLGAAKLLQQRRNELKGTVKLVFQPGEEGRGGAYHMV 180

Query: 181 KEGCVEKVEGIFGLHVAQDMSVGAIGSRPGPFTACSGRFLATIQGIGGHAALPHKARDPV 240
           KEG +E V+GIFGLHVAQDM++GAIGSRPGPFTA SGRFLAT+QG+GGHAALPH+A+DP+
Sbjct: 181 KEGAIENVKGIFGLHVAQDMTLGAIGSRPGPFTASSGRFLATVQGVGGHAALPHQAKDPL 240

Query: 241 LAMSSAIISLQYIISRETDPLDSRVITVGLVKGGETGNVIPETVTFGGTFRSITLEGLYN 300
           LA+SSAIISLQ+IISRETDP DSRVI+V LVKGGE GNVIPETVTFGGTFRS TLEGLYN
Sbjct: 241 LAVSSAIISLQHIISRETDPFDSRVISVALVKGGEAGNVIPETVTFGGTFRSKTLEGLYN 300

Query: 301 LKQRIQEVIEFQVGVHQCNATVDFLEEKARFYPPTINDQSLYNHIEKVGQHLLGGSSNVL 360
           LKQRIQ+VIEFQV V+ C+  VDF+EE ARFYPPTINDQSLY+H+  VGQHLLGG SNVL
Sbjct: 301 LKQRIQQVIEFQVAVYDCSVIVDFMEESARFYPPTINDQSLYDHVNNVGQHLLGGPSNVL 360

Query: 361 HLPNTMGAEDFSFYSQHIPAAFFMIGAKNDTMESGIPLHSPYLVLDEQVLPLGAALHAAV 420
           HLPNTMGAEDFSFYSQ IPA FFMIGAKNDTM+S IPLHSPYL+LDE+VLPLGAALHA V
Sbjct: 361 HLPNTMGAEDFSFYSQQIPAVFFMIGAKNDTMDSRIPLHSPYLILDEEVLPLGAALHAVV 420

Query: 421 AISYLDQHYYSVPSN 436
           AISYLDQ   SV SN
Sbjct: 421 AISYLDQ--LSVSSN 433

BLAST of HG10020086 vs. NCBI nr
Match: KAA0043848.1 (IAA-amino acid hydrolase ILR1-like 3 [Cucumis melo var. makuwa] >TYK25287.1 IAA-amino acid hydrolase ILR1-like 3 [Cucumis melo var. makuwa])

HSP 1 Score: 734.2 bits (1894), Expect = 6.6e-208
Identity = 358/415 (86.27%), Postives = 387/415 (93.25%), Query Frame = 0

Query: 21  LETEPPLELSRLTRELLESARNPEFFEWLVRARRKLHENPELSFEEFETSQFIRTELESL 80
           ++ EPPLELSRLTR+LLESAR+PEFFEWLVR RRKLHENPEL+FEEFETS+FIRTELES+
Sbjct: 1   MKAEPPLELSRLTRDLLESARDPEFFEWLVRTRRKLHENPELAFEEFETSEFIRTELESV 60

Query: 81  GINFTWPVAKTGIVASVGSGTHPWFALRADMDALPIQEKVEWEHKSKKDGKMHACGHDVH 140
           GINF WPVAKTGIVASVGSG HPWFALRADMDALPIQE VEWEHKSKKDGKMHACGHDVH
Sbjct: 61  GINFNWPVAKTGIVASVGSGAHPWFALRADMDALPIQEMVEWEHKSKKDGKMHACGHDVH 120

Query: 141 VTMLLGAAKLLQQRRNELKGTVKLVFQPGEEGRGGAYYMVKEGCVEKVEGIFGLHVAQDM 200
           VTMLLGAAKLLQQRRNELKGTVKLVFQPGEEGRGGAY+MVKEG +E V+GIFGLHVAQDM
Sbjct: 121 VTMLLGAAKLLQQRRNELKGTVKLVFQPGEEGRGGAYHMVKEGAIENVKGIFGLHVAQDM 180

Query: 201 SVGAIGSRPGPFTACSGRFLATIQGIGGHAALPHKARDPVLAMSSAIISLQYIISRETDP 260
           ++GAIGSRPGPFTA SGRFLAT+QG+GGHAALPH+A+DP+LA+SSAIISLQ+IISRETDP
Sbjct: 181 TLGAIGSRPGPFTASSGRFLATVQGVGGHAALPHQAKDPLLAVSSAIISLQHIISRETDP 240

Query: 261 LDSRVITVGLVKGGETGNVIPETVTFGGTFRSITLEGLYNLKQRIQEVIEFQVGVHQCNA 320
            DSRVI+V LVKGGE GNVIPETVTFGGTFRS TLEGLYNLKQRIQ+VIEFQV V+ C+ 
Sbjct: 241 FDSRVISVALVKGGEAGNVIPETVTFGGTFRSKTLEGLYNLKQRIQQVIEFQVAVYDCSV 300

Query: 321 TVDFLEEKARFYPPTINDQSLYNHIEKVGQHLLGGSSNVLHLPNTMGAEDFSFYSQHIPA 380
            VDF+EE ARFYPPTINDQSLY+H+  VGQHLLGG SNVLHLPNTMGAEDFSFYSQ IPA
Sbjct: 301 IVDFMEESARFYPPTINDQSLYDHVNNVGQHLLGGPSNVLHLPNTMGAEDFSFYSQQIPA 360

Query: 381 AFFMIGAKNDTMESGIPLHSPYLVLDEQVLPLGAALHAAVAISYLDQHYYSVPSN 436
            FFMIGAKNDTM+S IPLHSPYL+LDE+VLPLGAALHA VAISYLDQ   SV SN
Sbjct: 361 VFFMIGAKNDTMDSRIPLHSPYLILDEEVLPLGAALHAVVAISYLDQ--LSVSSN 413

BLAST of HG10020086 vs. NCBI nr
Match: XP_038904781.1 (IAA-amino acid hydrolase ILR1-like 3 [Benincasa hispida])

HSP 1 Score: 720.7 bits (1859), Expect = 7.6e-204
Identity = 354/435 (81.38%), Postives = 391/435 (89.89%), Query Frame = 0

Query: 1   MEKLLLCAFFFIFQFCISSPLETEPPLELSRLTRELLESARNPEFFEWLVRARRKLHENP 60
           ME+LLL AFFF+F FC SS L TEPPLELS LTRELLESARNPEFF+WLVRARRKLHENP
Sbjct: 1   MEQLLLLAFFFVFPFCTSSGLGTEPPLELSHLTRELLESARNPEFFDWLVRARRKLHENP 60

Query: 61  ELSFEEFETSQFIRTELESLGINFTWPVAKTGIVASVGSGTHPWFALRADMDALPIQEKV 120
           ELSFEEFETSQFIRTELESLGINFTWP AKTGIVASVGSG HPWFALRADMDALPIQE V
Sbjct: 61  ELSFEEFETSQFIRTELESLGINFTWPFAKTGIVASVGSGAHPWFALRADMDALPIQEMV 120

Query: 121 EWEHKSKKDGKMHACGHDVHVTMLLGAAKLLQQRRNELKGTVKLVFQPGEEGRGGAYYMV 180
           EWEHKSK DGKMHACGHD HVTMLLGAAKLLQQRRNELKGTVKLVFQPGEEGR GAY+M+
Sbjct: 121 EWEHKSKNDGKMHACGHDAHVTMLLGAAKLLQQRRNELKGTVKLVFQPGEEGRAGAYHML 180

Query: 181 KEGCVEKVEGIFGLHVAQDMSVGAIGSRPGPFTACSGRFLATIQGIGGHAALPHKARDPV 240
           KEG ++K +GIFGLH+  ++ VG IGSR GPF A SGRF+ATIQGIGGHAA+PH ARDPV
Sbjct: 181 KEGALDKFQGIFGLHIIPELPVGTIGSRAGPFMAGSGRFVATIQGIGGHAAMPHNARDPV 240

Query: 241 LAMSSAIISLQYIISRETDPLDSRVITVGLVKGGETGNVIPETVTFGGTFRSITLEGLYN 300
           LAMS+AIISLQ+IISRETDPLDSRVITVG VKGG+ GNVIP+TVTFGGTFRS+T+EGL  
Sbjct: 241 LAMSTAIISLQHIISRETDPLDSRVITVGFVKGGQAGNVIPKTVTFGGTFRSMTMEGLSY 300

Query: 301 LKQRIQEVIEFQVGVHQCNATVDFLEEKARFYPPTINDQSLYNHIEKVGQHLLGGSSNVL 360
           LKQRIQEVIE Q  VH+C A+VDF+EEK+ FYP T+ND++LY+H +KVG+HLLGG SNV 
Sbjct: 301 LKQRIQEVIEVQAAVHRCTASVDFMEEKSIFYPATVNDEALYSHAKKVGEHLLGGESNVH 360

Query: 361 HLPNTMGAEDFSFYSQHIPAAFFMIGAKNDTMESGIPLHSPYLVLDEQVLPLGAALHAAV 420
           HLP  M AEDFSFYSQ +PAAFFMIG KN+TM+SGIPLHSPY+VLDE+VLP+GAALHAAV
Sbjct: 361 HLPKIMAAEDFSFYSQQMPAAFFMIGVKNETMKSGIPLHSPYIVLDERVLPVGAALHAAV 420

Query: 421 AISYLDQHYYSVPSN 436
           AISYL++   SV SN
Sbjct: 421 AISYLNEQ--SVHSN 433

BLAST of HG10020086 vs. ExPASy Swiss-Prot
Match: Q8H3C9 (IAA-amino acid hydrolase ILR1-like 7 OS=Oryza sativa subsp. japonica OX=39947 GN=ILL7 PE=2 SV=1)

HSP 1 Score: 476.5 bits (1225), Expect = 3.3e-133
Identity = 235/402 (58.46%), Postives = 302/402 (75.12%), Query Frame = 0

Query: 35  ELLESARNPEFFEWLVRARRKLHENPELSFEEFETSQFIRTELESLGINFTWPVAKTGIV 94
           ELL +AR P F  WL   RR +H +PEL+FEE  TS+ +R EL+++G+ + WPVA+TG+V
Sbjct: 52  ELLSAARAPGFAAWLRGLRRSIHRHPELAFEEVRTSELVRAELDAIGVPYEWPVARTGVV 111

Query: 95  ASV----GSGTHPWFALRADMDALPIQEKVEWEHKSKKDGKMHACGHDVHVTMLLGAAKL 154
           A++    G+G    FALRADMDALP+QE V+WEHKS++ GKMHACGHD H TMLLGAAKL
Sbjct: 112 ATIAGGDGAGAGTVFALRADMDALPLQELVDWEHKSEESGKMHACGHDAHTTMLLGAAKL 171

Query: 155 LQQRRNELKGTVKLVFQPGEEGRGGAYYMVKEGCVEKVEGIFGLHVAQDMSVGAIGSRPG 214
           LQ ++++LKGTVKLVFQP EEG  GA Y+++EG ++ V  IFGLHV   + VG + SRPG
Sbjct: 172 LQSQKDDLKGTVKLVFQPAEEGYAGARYVLQEGVLDDVSAIFGLHVDPRIQVGTVTSRPG 231

Query: 215 PFTACSGRFLATIQGIGGHAALPHKARDPVLAMSSAIISLQYIISRETDPLDSRVITVGL 274
           PF A SGRFLATI G GGHAA PH A DP+L  SSAI+SLQ I++RETDPL++ VI+V  
Sbjct: 232 PFLAASGRFLATITGKGGHAAGPHNAVDPILTASSAIVSLQQIVARETDPLEAAVISVTF 291

Query: 275 VKGGETGNVIPETVTFGGTFRSITLEGLYNLKQRIQEVIEFQVGVHQCNATVDFLEEKAR 334
           +KGG+  NVIPE+V+FGGTFRS+T EGL  LK+RI+E++E    VH+C ATVDF+EE+  
Sbjct: 292 MKGGDAYNVIPESVSFGGTFRSLTSEGLSYLKKRIKEIVEAHATVHRCTATVDFMEEERI 351

Query: 335 FYPPTINDQSLYNHIEKVGQHLLGGSSNVLHLPNTMGAEDFSFYSQHIPAAFFMIGAKND 394
            YP T+ND+ +Y H   V   +LG     +  P  MG+EDF+FY+Q  PAAFFMIG  N+
Sbjct: 352 PYPATVNDEGMYRHARAVAVDVLGEDGVKVGTP-FMGSEDFAFYAQRFPAAFFMIGVGNE 411

Query: 395 -TMESGIPLHSPYLVLDEQVLPLGAALHAAVAISYLDQHYYS 432
            TM    PLHSP+ V+DE VLP+GAALHAAVA+ YL++H ++
Sbjct: 412 TTMRKVYPLHSPHFVVDEDVLPVGAALHAAVAMEYLNKHAFT 452

BLAST of HG10020086 vs. ExPASy Swiss-Prot
Match: P54968 (IAA-amino acid hydrolase ILR1 OS=Arabidopsis thaliana OX=3702 GN=ILR1 PE=1 SV=2)

HSP 1 Score: 468.4 bits (1204), Expect = 8.9e-131
Identity = 232/429 (54.08%), Postives = 310/429 (72.26%), Query Frame = 0

Query: 8   AFFFIF-QFCISSPLETEPPLE----LSRLTRELLESARNPEFFEWLVRARRKLHENPEL 67
           +FF IF  F    PL +    +    L  L R +L SA++PEFFEW+   RRK+HENPE 
Sbjct: 6   SFFVIFVTFFFLPPLSSAGSYDSGSGLESLARGMLHSAKDPEFFEWMRGIRRKIHENPET 65

Query: 68  SFEEFETSQFIRTELESLGINFTWPVAKTGIVASVGSGTHPWFALRADMDALPIQEKVEW 127
            F+EF+TSQ +R EL+SLG+ + +PVAKTG+VA +GS + P F LRADMDALP+QE VEW
Sbjct: 66  GFQEFKTSQLVRDELDSLGVKYKYPVAKTGVVAWIGSCSKPVFGLRADMDALPLQELVEW 125

Query: 128 EHKSKKDGKMHACGHDVHVTMLLGAAKLLQQRRNELKGTVKLVFQPGEEGRGGAYYMVKE 187
           E KSK DGKMHACGHD HV MLLGAAKLLQ  ++ +KGTVKLVFQPGEEG  GAY M+K+
Sbjct: 126 ESKSKVDGKMHACGHDTHVAMLLGAAKLLQTTKHLIKGTVKLVFQPGEEGYAGAYEMLKD 185

Query: 188 GCVEKVEGIFGLHVAQDMSVGAIGSRPGPFTACSGRFLATIQGIGGHAALPHKARDPVLA 247
             ++ ++GI  +HV   +  G IGSRPG   A +G F  T+ G G HAA PH ++DPVLA
Sbjct: 186 EILDDLDGILSVHVFPSIPSGGIGSRPGTVLAGAGLFTVTVHGQGSHAATPHFSKDPVLA 245

Query: 248 MSSAIISLQYIISRETDPLDSRVITVGLVKGGETGNVIPETVTFGGTFRSITLEGLYNLK 307
            SSA+++LQ I+SRE DPL++ V+TVG ++GG   NVIP++  FGGTFRS++ +GL  ++
Sbjct: 246 ASSAVVALQQIVSRELDPLEAGVVTVGYIEGGHAQNVIPQSAKFGGTFRSLSNDGLLFIQ 305

Query: 308 QRIQEVIEFQVGVHQCNATVDFLEEKARFYPPTINDQSLYNHIEKVGQHLLGGSSNVLHL 367
           +RI+E+ E Q  V++C A V+F E+K   +P   ND+ LY H +KV + ++ G +N    
Sbjct: 306 RRIKEISEAQASVYRCKAEVNFEEKKPSLHPVMNNDEGLYEHGKKVAEAMI-GKNNFHDF 365

Query: 368 PNTMGAEDFSFYSQHIPAAFFMIGAKNDTMESGIPLHSPYLVLDEQVLPLGAALHAAVAI 427
           P TMG EDFSF++Q   AA F++G KN+T+ +G PLHSPY  +DE+ LP+GAALHAA+A+
Sbjct: 366 PVTMGGEDFSFFTQKTKAAIFVLGVKNETLGAGKPLHSPYFFVDEEALPVGAALHAAMAV 425

Query: 428 SYLDQHYYS 432
           SYLD+H +S
Sbjct: 426 SYLDEHGHS 433

BLAST of HG10020086 vs. ExPASy Swiss-Prot
Match: Q851L5 (IAA-amino acid hydrolase ILR1-like 3 OS=Oryza sativa subsp. japonica OX=39947 GN=ILL3 PE=2 SV=1)

HSP 1 Score: 467.6 bits (1202), Expect = 1.5e-130
Identity = 234/404 (57.92%), Postives = 296/404 (73.27%), Query Frame = 0

Query: 32  LTRELLESARNPEFFEWLVRARRKLHENPELSFEEFETSQFIRTELESLGINFTWPVAKT 91
           L RELLE+AR PEF  WL   RR++H++PEL+F+E  TS  +R EL++LG+ + WPVA+T
Sbjct: 9   LGRELLEAARAPEFAGWLRGLRRRIHQHPELAFQEHRTSALVRAELDALGVAYVWPVAQT 68

Query: 92  GIVASV--GSGTHPWFALRADMDALPIQEKVEWEHKSKKDGKMHACGHDVHVTMLLGAAK 151
           G+VA+V   +G  P F LRADMDALPIQE VEWE KS +DGKMHACGHDVHV MLLGAAK
Sbjct: 69  GVVATVVGAAGPGPVFGLRADMDALPIQEMVEWEFKSLEDGKMHACGHDVHVAMLLGAAK 128

Query: 152 LLQQRRNELKGTVKLVFQPGEEGRGGAYYMVKEGCVEKVEGIFGLHVAQDMSVGAIGSRP 211
           LLQ RR+   G VKLVFQP EEG  G YY+++EG V+ V+GIFG+HV   +  G + SRP
Sbjct: 129 LLQSRRDHFNGKVKLVFQPAEEGYAGGYYVLEEGAVDDVQGIFGMHVDAGLPAGVVASRP 188

Query: 212 GPFTACSGRFLATIQGIGGHAALPHKARDPVLAMSSAIISLQYIISRETDPLDSRVITVG 271
           GPF A S RF ATI G GGHAA PH A DP++A+SSA++SLQ I++RETDPL   V++V 
Sbjct: 189 GPFLAGSARFTATINGKGGHAAAPHHAVDPIVAVSSAVLSLQQIVARETDPLQGAVVSVT 248

Query: 272 LVKGGETGNVIPETVTFGGTFRSITLEGLYNLKQRIQEVIEFQVGVHQCNATVDFLEEKA 331
            +KGGE  NVIPE+VT GGT RS+T +G+  L +RI+EVIE Q  V++C A VDF+E+K 
Sbjct: 249 TIKGGEAFNVIPESVTLGGTLRSMTTDGMSYLMKRIREVIEGQAAVNRCTAAVDFMEDKL 308

Query: 332 RFYPPTINDQSLYNHIEKVGQHLLGGSSNVLHLPNTMGAEDFSFYSQHIPAAFFMIGAKN 391
             YP T+ND+ +Y H + V + +L G +NV   P  MGAEDF FY+Q IPAAFF IG  N
Sbjct: 309 PPYPATVNDEEMYAHAKAVAESML-GEANVKLSPQGMGAEDFGFYAQRIPAAFFGIGVGN 368

Query: 392 D-----TMESGIPLHSPYLVLDEQVLPLGAALHAAVAISYLDQH 429
           D        +   LHSP+ V+DE+ LP+GAA HAAVAI YL+++
Sbjct: 369 DGGGMAETTTKNQLHSPHFVVDEEALPVGAAFHAAVAIEYLNKN 411

BLAST of HG10020086 vs. ExPASy Swiss-Prot
Match: Q8H3C7 (IAA-amino acid hydrolase ILR1-like 9 OS=Oryza sativa subsp. japonica OX=39947 GN=ILL9 PE=2 SV=2)

HSP 1 Score: 458.4 bits (1178), Expect = 9.2e-128
Identity = 225/404 (55.69%), Postives = 291/404 (72.03%), Query Frame = 0

Query: 35  ELLESARNPEFFEWLVRARRKLHENPELSFEEFETSQFIRTELESLGINFTWPVAKTGIV 94
           +LL +AR P   EWL   RR++H +PEL+FEE  TS+ +R EL+++G+ + WPVA+TG+V
Sbjct: 38  DLLAAAREPGMAEWLRGVRRRIHRHPELAFEEVRTSELVRAELDAIGVPYQWPVARTGVV 97

Query: 95  ASV---GSGTHPWFALRADMDALPIQEKVEWEHKSKKDGKMHACGHDVHVTMLLGAAKLL 154
           A++   G G  P  ALRADMDALP+QE V+WEHKS+++GKMHACGHD H  MLLGAAKLL
Sbjct: 98  ATIAGGGGGDGPVVALRADMDALPVQELVDWEHKSQENGKMHACGHDAHTAMLLGAAKLL 157

Query: 155 QQRRNELKGTVKLVFQPGEEGRGGAYYMVKEGCVEKVEGIFGLHVAQDMSVGAIGSRPGP 214
           Q+R+NELKGTVKLVFQP EEG  GAYY+++EG ++ V  +FG+HV   + VG + +RPGP
Sbjct: 158 QKRKNELKGTVKLVFQPAEEGSAGAYYVLQEGVLDDVSAMFGMHVDPALPVGVVAARPGP 217

Query: 215 FTACSGRFLATIQGIGGHAALPHKARDPVLAMSSAIISLQYIISRETDPLDSRVITVGLV 274
           F A SGRFLATI G GGHAA PH A DPV+A S+AI+SLQ I++RE DPL   V+++  V
Sbjct: 218 FAATSGRFLATITGKGGHAAFPHDAIDPVVAASNAILSLQQIVAREIDPLQGAVVSITFV 277

Query: 275 KGGETGNVIPETVTFGGTFRSITLEGLYNLKQRIQEVIEFQVGVHQCNATVDFLEEKARF 334
           KGGE  NVIP++V FGGT RS+T EGL  L +RI+E++E Q  V++C   VDF+EE  R 
Sbjct: 278 KGGEAYNVIPQSVEFGGTMRSMTDEGLAYLMKRIKEIVEGQAAVNRCGGGVDFMEESMRP 337

Query: 335 YPPTINDQSLYNHIEKVGQHLLGGSSNVLHLPNTMGAEDFSFYSQHIPAAFFMIGAKNDT 394
           YP  +ND+ +Y H     + LL G+  V   P  MGAEDF FY+  +P+AFF IG  N T
Sbjct: 338 YPAVVNDEGMYAHARASAERLL-GAGGVRVAPQLMGAEDFGFYAARMPSAFFTIGVGNAT 397

Query: 395 MES---GIPLHSPYLVLDEQVLPLGAALHAAVAISYLDQHYYSV 433
             S       HSP+ V+DE  LP+GAA+HAAVAI YL +H  S+
Sbjct: 398 TSSARAAHTTHSPHFVIDEAALPVGAAVHAAVAIDYLSKHASSM 440

BLAST of HG10020086 vs. ExPASy Swiss-Prot
Match: Q8H3C8 (IAA-amino acid hydrolase ILR1-like 8 OS=Oryza sativa subsp. japonica OX=39947 GN=ILL8 PE=2 SV=1)

HSP 1 Score: 457.2 bits (1175), Expect = 2.0e-127
Identity = 227/431 (52.67%), Postives = 307/431 (71.23%), Query Frame = 0

Query: 4   LLLCAFFFIFQFCISSPLETEPPLELSRLTRELLESARNPEFFEWLVRARRKLHENPELS 63
           LL+ A   +  F       T     L  L  +LL +A    F  WL   RR++H+ PEL+
Sbjct: 12  LLVAAAAAVVLFAHLPTTTTAASPALKALGEDLLAAAGAAGFAGWLSGLRRRIHQRPELA 71

Query: 64  FEEFETSQFIRTELESLGINFTWPVAKTGIVASV--GSGTHPWFALRADMDALPIQEKVE 123
           F+E  TS+ +R EL+++G+ + WPVA+TG+VA++  G+G  P  ALRADMDALP+QE V+
Sbjct: 72  FQEVRTSELVRAELDAIGVPYAWPVARTGVVATIDGGAGAGPVVALRADMDALPLQELVD 131

Query: 124 WEHKSKKDGKMHACGHDVHVTMLLGAAKLLQQRRNELKGTVKLVFQPGEEGRGGAYYMVK 183
           WE KS++ GKMHACGHD HVTMLLGAAKLLQ R++ELKGT+KLVFQP EEG  GAY++++
Sbjct: 132 WEFKSQEKGKMHACGHDAHVTMLLGAAKLLQSRKDELKGTIKLVFQPAEEGHAGAYHVLE 191

Query: 184 EGCVEKVEGIFGLHVAQDMSVGAIGSRPGPFTACSGRFLATIQGIGGHAALPHKARDPVL 243
            G ++ V  IFGLHV  ++ VG + SRPGPF + + RF AT  G GGHA +PH A DPV+
Sbjct: 192 SGLLDDVSVIFGLHVIPNLPVGVVASRPGPFMSAAARFAATFTGKGGHAGVPHDAVDPVV 251

Query: 244 AMSSAIISLQYIISRETDPLDSRVITVGLVKGGETGNVIPETVTFGGTFRSITLEGLYNL 303
           A+SSA++SLQ ++SRETDPL++ V+++ ++KGG+  NVIPE+ + GGTFRS+T EGL  L
Sbjct: 252 AVSSAVLSLQQLVSRETDPLEAAVVSITILKGGDAYNVIPESASLGGTFRSMTDEGLAYL 311

Query: 304 KQRIQEVIEFQVGVHQCNATVDFLEEKARFYPPTINDQSLYNHIEKVGQHLLGGSSNVLH 363
            +RI+E+IE Q GV++C A VDFLEE+ R YP T+ND  +Y H + V + +L G +NV  
Sbjct: 312 MKRIREIIEAQAGVNRCAAAVDFLEEELRPYPATVNDDGMYGHAKAVAEAML-GEANVRV 371

Query: 364 LPNTMGAEDFSFYSQHIPAAFFMIGAKNDTMESGI----PLHSPYLVLDEQVLPLGAALH 423
              +MG EDF+FY++  P AFF IG  N+T         P+HSP+ VLDE+ LP+GAALH
Sbjct: 372 AARSMGGEDFAFYARRSPGAFFFIGVGNETTMGPAAAVRPVHSPHFVLDERALPVGAALH 431

Query: 424 AAVAISYLDQH 429
           AAVAI YL++H
Sbjct: 432 AAVAIEYLNKH 441

BLAST of HG10020086 vs. ExPASy TrEMBL
Match: A0A0A0LGQ7 (M20_dimer domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G778230 PE=3 SV=1)

HSP 1 Score: 780.4 bits (2014), Expect = 3.9e-222
Identity = 381/435 (87.59%), Postives = 409/435 (94.02%), Query Frame = 0

Query: 1   MEKLLLCAFFFIFQFCISSPLETEPPLELSRLTRELLESARNPEFFEWLVRARRKLHENP 60
           ME++++   FFIF FCISS +E EPPLELSRLTRELLESAR+PEFFEWLV+ARRKLHENP
Sbjct: 1   MEQIIVWGLFFIFPFCISSGMEAEPPLELSRLTRELLESARDPEFFEWLVKARRKLHENP 60

Query: 61  ELSFEEFETSQFIRTELESLGINFTWPVAKTGIVASVGSGTHPWFALRADMDALPIQEKV 120
           EL+FEEFETS+FIRTELES+GINF WP+AKTGIVASVGSG HPWFALRADMDALPIQE V
Sbjct: 61  ELAFEEFETSEFIRTELESVGINFNWPLAKTGIVASVGSGAHPWFALRADMDALPIQEMV 120

Query: 121 EWEHKSKKDGKMHACGHDVHVTMLLGAAKLLQQRRNELKGTVKLVFQPGEEGRGGAYYMV 180
           EWEHKSKKDGKMHACGHDVHVTMLLGAAKLLQQRRNELKGTVKLVFQPGEEGRGGAYYMV
Sbjct: 121 EWEHKSKKDGKMHACGHDVHVTMLLGAAKLLQQRRNELKGTVKLVFQPGEEGRGGAYYMV 180

Query: 181 KEGCVEKVEGIFGLHVAQDMSVGAIGSRPGPFTACSGRFLATIQGIGGHAALPHKARDPV 240
           KEG +E V+GIFGLHVAQDM++GAIGSRPGPFTACSGRFLATIQGIGGHAALPH+A+DP+
Sbjct: 181 KEGAIENVKGIFGLHVAQDMTLGAIGSRPGPFTACSGRFLATIQGIGGHAALPHQAKDPL 240

Query: 241 LAMSSAIISLQYIISRETDPLDSRVITVGLVKGGETGNVIPETVTFGGTFRSITLEGLYN 300
           LAMSSAIISLQ+IISRETDP DSRVI+VGLVKGGE  NVIPETVTFGGTFRS TLEGLYN
Sbjct: 241 LAMSSAIISLQHIISRETDPFDSRVISVGLVKGGEARNVIPETVTFGGTFRSKTLEGLYN 300

Query: 301 LKQRIQEVIEFQVGVHQCNATVDFLEEKARFYPPTINDQSLYNHIEKVGQHLLGGSSNVL 360
           LK RIQ+VIEFQV V+ C+A VDF+EEKARFYPPTINDQSLY+H+  VGQHLLGG SNVL
Sbjct: 301 LKHRIQQVIEFQVAVYGCSAIVDFMEEKARFYPPTINDQSLYDHVNNVGQHLLGGPSNVL 360

Query: 361 HLPNTMGAEDFSFYSQHIPAAFFMIGAKNDTMESGIPLHSPYLVLDEQVLPLGAALHAAV 420
           HLPNTMGAEDFSFYSQHIPAAFFMIGAKNDTMESGIPLHSPYLVLDE VLPLGAALHAAV
Sbjct: 361 HLPNTMGAEDFSFYSQHIPAAFFMIGAKNDTMESGIPLHSPYLVLDEHVLPLGAALHAAV 420

Query: 421 AISYLDQHYYSVPSN 436
           AISYLDQ ++ V SN
Sbjct: 421 AISYLDQQHHFVSSN 435

BLAST of HG10020086 vs. ExPASy TrEMBL
Match: A0A1S3B6R3 (IAA-amino acid hydrolase ILR1-like 3 OS=Cucumis melo OX=3656 GN=LOC103486646 PE=3 SV=1)

HSP 1 Score: 751.9 bits (1940), Expect = 1.5e-213
Identity = 368/435 (84.60%), Postives = 400/435 (91.95%), Query Frame = 0

Query: 1   MEKLLLCAFFFIFQFCISSPLETEPPLELSRLTRELLESARNPEFFEWLVRARRKLHENP 60
           ME +++   FFI  FCISS ++ EPPLELSRLTR+LLESAR+PEFFEWLVR RRKLHENP
Sbjct: 1   MEIIIVWGLFFILPFCISSGMKAEPPLELSRLTRDLLESARDPEFFEWLVRTRRKLHENP 60

Query: 61  ELSFEEFETSQFIRTELESLGINFTWPVAKTGIVASVGSGTHPWFALRADMDALPIQEKV 120
           EL+FEEFETS+FIRTELES+GINF WPVAKTGIVASVGSG HPWFALRADMDALPIQE V
Sbjct: 61  ELAFEEFETSEFIRTELESVGINFNWPVAKTGIVASVGSGAHPWFALRADMDALPIQEMV 120

Query: 121 EWEHKSKKDGKMHACGHDVHVTMLLGAAKLLQQRRNELKGTVKLVFQPGEEGRGGAYYMV 180
           EWEHKSKKDGKMHACGHDVHVTMLLGAAKLLQQRRNELKGTVKLVFQPGEEGRGGAY+MV
Sbjct: 121 EWEHKSKKDGKMHACGHDVHVTMLLGAAKLLQQRRNELKGTVKLVFQPGEEGRGGAYHMV 180

Query: 181 KEGCVEKVEGIFGLHVAQDMSVGAIGSRPGPFTACSGRFLATIQGIGGHAALPHKARDPV 240
           KEG +E V+GIFGLHVAQDM++GAIGSRPGPFTA SGRFLAT+QG+GGHAALPH+A+DP+
Sbjct: 181 KEGAIENVKGIFGLHVAQDMTLGAIGSRPGPFTASSGRFLATVQGVGGHAALPHQAKDPL 240

Query: 241 LAMSSAIISLQYIISRETDPLDSRVITVGLVKGGETGNVIPETVTFGGTFRSITLEGLYN 300
           LA+SSAIISLQ+IISRETDP DSRVI+V LVKGGE GNVIPETVTFGGTFRS TLEGLYN
Sbjct: 241 LAVSSAIISLQHIISRETDPFDSRVISVALVKGGEAGNVIPETVTFGGTFRSKTLEGLYN 300

Query: 301 LKQRIQEVIEFQVGVHQCNATVDFLEEKARFYPPTINDQSLYNHIEKVGQHLLGGSSNVL 360
           LKQRIQ+VIEFQV V+ C+  VDF+EE ARFYPPTINDQSLY+H+  VGQHLLGG SNVL
Sbjct: 301 LKQRIQQVIEFQVAVYDCSVIVDFMEESARFYPPTINDQSLYDHVNNVGQHLLGGPSNVL 360

Query: 361 HLPNTMGAEDFSFYSQHIPAAFFMIGAKNDTMESGIPLHSPYLVLDEQVLPLGAALHAAV 420
           HLPNTMGAEDFSFYSQ IPA FFMIGAKNDTM+S IPLHSPYL+LDE+VLPLGAALHA V
Sbjct: 361 HLPNTMGAEDFSFYSQQIPAVFFMIGAKNDTMDSRIPLHSPYLILDEEVLPLGAALHAVV 420

Query: 421 AISYLDQHYYSVPSN 436
           AISYLDQ   SV SN
Sbjct: 421 AISYLDQ--LSVSSN 433

BLAST of HG10020086 vs. ExPASy TrEMBL
Match: A0A5A7TLW9 (IAA-amino acid hydrolase ILR1-like 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G004230 PE=3 SV=1)

HSP 1 Score: 734.2 bits (1894), Expect = 3.2e-208
Identity = 358/415 (86.27%), Postives = 387/415 (93.25%), Query Frame = 0

Query: 21  LETEPPLELSRLTRELLESARNPEFFEWLVRARRKLHENPELSFEEFETSQFIRTELESL 80
           ++ EPPLELSRLTR+LLESAR+PEFFEWLVR RRKLHENPEL+FEEFETS+FIRTELES+
Sbjct: 1   MKAEPPLELSRLTRDLLESARDPEFFEWLVRTRRKLHENPELAFEEFETSEFIRTELESV 60

Query: 81  GINFTWPVAKTGIVASVGSGTHPWFALRADMDALPIQEKVEWEHKSKKDGKMHACGHDVH 140
           GINF WPVAKTGIVASVGSG HPWFALRADMDALPIQE VEWEHKSKKDGKMHACGHDVH
Sbjct: 61  GINFNWPVAKTGIVASVGSGAHPWFALRADMDALPIQEMVEWEHKSKKDGKMHACGHDVH 120

Query: 141 VTMLLGAAKLLQQRRNELKGTVKLVFQPGEEGRGGAYYMVKEGCVEKVEGIFGLHVAQDM 200
           VTMLLGAAKLLQQRRNELKGTVKLVFQPGEEGRGGAY+MVKEG +E V+GIFGLHVAQDM
Sbjct: 121 VTMLLGAAKLLQQRRNELKGTVKLVFQPGEEGRGGAYHMVKEGAIENVKGIFGLHVAQDM 180

Query: 201 SVGAIGSRPGPFTACSGRFLATIQGIGGHAALPHKARDPVLAMSSAIISLQYIISRETDP 260
           ++GAIGSRPGPFTA SGRFLAT+QG+GGHAALPH+A+DP+LA+SSAIISLQ+IISRETDP
Sbjct: 181 TLGAIGSRPGPFTASSGRFLATVQGVGGHAALPHQAKDPLLAVSSAIISLQHIISRETDP 240

Query: 261 LDSRVITVGLVKGGETGNVIPETVTFGGTFRSITLEGLYNLKQRIQEVIEFQVGVHQCNA 320
            DSRVI+V LVKGGE GNVIPETVTFGGTFRS TLEGLYNLKQRIQ+VIEFQV V+ C+ 
Sbjct: 241 FDSRVISVALVKGGEAGNVIPETVTFGGTFRSKTLEGLYNLKQRIQQVIEFQVAVYDCSV 300

Query: 321 TVDFLEEKARFYPPTINDQSLYNHIEKVGQHLLGGSSNVLHLPNTMGAEDFSFYSQHIPA 380
            VDF+EE ARFYPPTINDQSLY+H+  VGQHLLGG SNVLHLPNTMGAEDFSFYSQ IPA
Sbjct: 301 IVDFMEESARFYPPTINDQSLYDHVNNVGQHLLGGPSNVLHLPNTMGAEDFSFYSQQIPA 360

Query: 381 AFFMIGAKNDTMESGIPLHSPYLVLDEQVLPLGAALHAAVAISYLDQHYYSVPSN 436
            FFMIGAKNDTM+S IPLHSPYL+LDE+VLPLGAALHA VAISYLDQ   SV SN
Sbjct: 361 VFFMIGAKNDTMDSRIPLHSPYLILDEEVLPLGAALHAVVAISYLDQ--LSVSSN 413

BLAST of HG10020086 vs. ExPASy TrEMBL
Match: A0A0A0LEN6 (M20_dimer domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G778240 PE=3 SV=1)

HSP 1 Score: 711.8 bits (1836), Expect = 1.7e-201
Identity = 350/435 (80.46%), Postives = 386/435 (88.74%), Query Frame = 0

Query: 1   MEKLLLCAFFFIFQFCISSPLETEPPLELSRLTRELLESARNPEFFEWLVRARRKLHENP 60
           ME++L+ A FFIF F ISS L TEPPLELS LT ELLESARNP+FF+WLVRARRKLHENP
Sbjct: 1   MERILVRALFFIFPFLISSALGTEPPLELSHLTLELLESARNPKFFDWLVRARRKLHENP 60

Query: 61  ELSFEEFETSQFIRTELESLGINFTWPVAKTGIVASVGSGTHPWFALRADMDALPIQEKV 120
           ELSFEEFETSQFIRTELESLGINFTWPVAKTGIVAS+GSG HPWFALRADMDALPIQE V
Sbjct: 61  ELSFEEFETSQFIRTELESLGINFTWPVAKTGIVASIGSGAHPWFALRADMDALPIQEMV 120

Query: 121 EWEHKSKKDGKMHACGHDVHVTMLLGAAKLLQQRRNELKGTVKLVFQPGEEGRGGAYYMV 180
           EWEHKSKKDGKMHACGHD HVTMLLGAAKLLQQRRNELKGTVKLVFQPGEEGR GAY+M+
Sbjct: 121 EWEHKSKKDGKMHACGHDAHVTMLLGAAKLLQQRRNELKGTVKLVFQPGEEGRAGAYHML 180

Query: 181 KEGCVEKVEGIFGLHVAQDMSVGAIGSRPGPFTACSGRFLATIQGIGGHAALPHKARDPV 240
           KEG ++K +GIFGLH+  D+ +G IGSR GPF A SGRF ATIQGIGGHAA PHKARDPV
Sbjct: 181 KEGALDKFQGIFGLHIIPDLPIGTIGSRAGPFMAGSGRFQATIQGIGGHAAWPHKARDPV 240

Query: 241 LAMSSAIISLQYIISRETDPLDSRVITVGLVKGGETGNVIPETVTFGGTFRSITLEGLYN 300
           LAMSSAI+SLQ+IISRETDPLDSRVITVG VKGG+ GNVIPET TFGGTFRS+T+EGL  
Sbjct: 241 LAMSSAIVSLQHIISRETDPLDSRVITVGFVKGGQAGNVIPETATFGGTFRSMTVEGLSY 300

Query: 301 LKQRIQEVIEFQVGVHQCNATVDFLEEKARFYPPTINDQSLYNHIEKVGQHLLGGSSNVL 360
           L++RIQEVIE Q  VHQCNATVDF+E+K  FYP T+ND+ LY+H +KVG+HLLGG SNV 
Sbjct: 301 LQKRIQEVIEVQAAVHQCNATVDFMEDKLIFYPATVNDEGLYSHAKKVGEHLLGGESNVH 360

Query: 361 HLPNTMGAEDFSFYSQHIPAAFFMIGAKNDTMESGIPLHSPYLVLDEQVLPLGAALHAAV 420
           HL   M AEDFSFYSQ +PAAFFMIG KN+TM+SG PLHSPY+ +DE+VLP+GAALHAAV
Sbjct: 361 HLSMVMAAEDFSFYSQKMPAAFFMIGVKNETMKSGTPLHSPYITIDERVLPVGAALHAAV 420

Query: 421 AISYLDQHYYSVPSN 436
           AISYLD+H  SV SN
Sbjct: 421 AISYLDEH--SVGSN 433

BLAST of HG10020086 vs. ExPASy TrEMBL
Match: A0A6J1KSE7 (IAA-amino acid hydrolase ILR1-like 3 OS=Cucurbita maxima OX=3661 GN=LOC111498280 PE=3 SV=1)

HSP 1 Score: 695.7 bits (1794), Expect = 1.3e-196
Identity = 342/436 (78.44%), Postives = 387/436 (88.76%), Query Frame = 0

Query: 1   MEKLLLCAFFFI-FQFCISSPLETEPPLELSRLTRELLESARNPEFFEWLVRARRKLHEN 60
           ME++ L A FF+ F FC+SS  ETEPPLE+  LT ELLESAR PEFF+WLVR+RRKLHEN
Sbjct: 1   MERMFLWALFFVLFSFCVSSGKETEPPLEMEHLTSELLESARTPEFFDWLVRSRRKLHEN 60

Query: 61  PELSFEEFETSQFIRTELESLGINFTWPVAKTGIVASVGSGTHPWFALRADMDALPIQEK 120
           PELSF+EFETS+FIRTELESLG+NFTWP+AKTGIVAS+GSG  PWFALRADMDALPIQE 
Sbjct: 61  PELSFQEFETSEFIRTELESLGVNFTWPIAKTGIVASIGSGAQPWFALRADMDALPIQEM 120

Query: 121 VEWEHKSKKDGKMHACGHDVHVTMLLGAAKLLQQRRNELKGTVKLVFQPGEEGRGGAYYM 180
           VEWEHKSK DGKMHACGHDVHVTMLLGAAKLLQQRRNELKGTVKLVFQPGEEG  GAY+M
Sbjct: 121 VEWEHKSKNDGKMHACGHDVHVTMLLGAAKLLQQRRNELKGTVKLVFQPGEEGAAGAYHM 180

Query: 181 VKEGCVEKVEGIFGLHVAQDMSVGAIGSRPGPFTACSGRFLATIQGIGGHAALPHKARDP 240
           +KEG ++K +GIFGLHVA D+ +G IGSR GPF A SGRFLATIQG GGHAA+PHKA+DP
Sbjct: 181 LKEGALDKFQGIFGLHVAPDLPIGTIGSRAGPFMAGSGRFLATIQGSGGHAAMPHKAKDP 240

Query: 241 VLAMSSAIISLQYIISRETDPLDSRVITVGLVKGGETGNVIPETVTFGGTFRSITLEGLY 300
           VLAMS AIISLQ+IISRETDPL+SRVITVG VKGG+ GNVIPETVTFGGTFRS+T+EGL 
Sbjct: 241 VLAMSLAIISLQHIISRETDPLESRVITVGFVKGGQAGNVIPETVTFGGTFRSMTVEGLS 300

Query: 301 NLKQRIQEVIEFQVGVHQCNATVDFLEEKARFYPPTINDQSLYNHIEKVGQHLLGGSSNV 360
            L+QRI+EVIEFQ  VHQCNATVDF+EEK+R YP T+ND++LY+H +KVG+HLLGG SNV
Sbjct: 301 YLQQRIKEVIEFQSAVHQCNATVDFMEEKSRAYPATVNDEALYSHAKKVGEHLLGGESNV 360

Query: 361 LHLPNTMGAEDFSFYSQHIPAAFFMIGAKNDTMESGIPLHSPYLVLDEQVLPLGAALHAA 420
             LP  M AEDFSFY+Q +PAAFFMIGAKN++M+SGI LHSPY+VLDE+VLP+G+ALHAA
Sbjct: 361 RLLPMFMAAEDFSFYAQKMPAAFFMIGAKNESMKSGIQLHSPYIVLDEKVLPVGSALHAA 420

Query: 421 VAISYLDQHYYSVPSN 436
           VAISYLD    SV SN
Sbjct: 421 VAISYLDGQ--SVRSN 434

BLAST of HG10020086 vs. TAIR 10
Match: AT3G02875.1 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 468.4 bits (1204), Expect = 6.3e-132
Identity = 232/429 (54.08%), Postives = 310/429 (72.26%), Query Frame = 0

Query: 8   AFFFIF-QFCISSPLETEPPLE----LSRLTRELLESARNPEFFEWLVRARRKLHENPEL 67
           +FF IF  F    PL +    +    L  L R +L SA++PEFFEW+   RRK+HENPE 
Sbjct: 6   SFFVIFVTFFFLPPLSSAGSYDSGSGLESLARGMLHSAKDPEFFEWMRGIRRKIHENPET 65

Query: 68  SFEEFETSQFIRTELESLGINFTWPVAKTGIVASVGSGTHPWFALRADMDALPIQEKVEW 127
            F+EF+TSQ +R EL+SLG+ + +PVAKTG+VA +GS + P F LRADMDALP+QE VEW
Sbjct: 66  GFQEFKTSQLVRDELDSLGVKYKYPVAKTGVVAWIGSCSKPVFGLRADMDALPLQELVEW 125

Query: 128 EHKSKKDGKMHACGHDVHVTMLLGAAKLLQQRRNELKGTVKLVFQPGEEGRGGAYYMVKE 187
           E KSK DGKMHACGHD HV MLLGAAKLLQ  ++ +KGTVKLVFQPGEEG  GAY M+K+
Sbjct: 126 ESKSKVDGKMHACGHDTHVAMLLGAAKLLQTTKHLIKGTVKLVFQPGEEGYAGAYEMLKD 185

Query: 188 GCVEKVEGIFGLHVAQDMSVGAIGSRPGPFTACSGRFLATIQGIGGHAALPHKARDPVLA 247
             ++ ++GI  +HV   +  G IGSRPG   A +G F  T+ G G HAA PH ++DPVLA
Sbjct: 186 EILDDLDGILSVHVFPSIPSGGIGSRPGTVLAGAGLFTVTVHGQGSHAATPHFSKDPVLA 245

Query: 248 MSSAIISLQYIISRETDPLDSRVITVGLVKGGETGNVIPETVTFGGTFRSITLEGLYNLK 307
            SSA+++LQ I+SRE DPL++ V+TVG ++GG   NVIP++  FGGTFRS++ +GL  ++
Sbjct: 246 ASSAVVALQQIVSRELDPLEAGVVTVGYIEGGHAQNVIPQSAKFGGTFRSLSNDGLLFIQ 305

Query: 308 QRIQEVIEFQVGVHQCNATVDFLEEKARFYPPTINDQSLYNHIEKVGQHLLGGSSNVLHL 367
           +RI+E+ E Q  V++C A V+F E+K   +P   ND+ LY H +KV + ++ G +N    
Sbjct: 306 RRIKEISEAQASVYRCKAEVNFEEKKPSLHPVMNNDEGLYEHGKKVAEAMI-GKNNFHDF 365

Query: 368 PNTMGAEDFSFYSQHIPAAFFMIGAKNDTMESGIPLHSPYLVLDEQVLPLGAALHAAVAI 427
           P TMG EDFSF++Q   AA F++G KN+T+ +G PLHSPY  +DE+ LP+GAALHAA+A+
Sbjct: 366 PVTMGGEDFSFFTQKTKAAIFVLGVKNETLGAGKPLHSPYFFVDEEALPVGAALHAAMAV 425

Query: 428 SYLDQHYYS 432
           SYLD+H +S
Sbjct: 426 SYLDEHGHS 433

BLAST of HG10020086 vs. TAIR 10
Match: AT1G51760.1 (peptidase M20/M25/M40 family protein )

HSP 1 Score: 431.4 bits (1108), Expect = 8.6e-121
Identity = 210/397 (52.90%), Postives = 280/397 (70.53%), Query Frame = 0

Query: 29  LSRLTRELLESARNPEFFEWLVRARRKLHENPELSFEEFETSQFIRTELESLGINFTWPV 88
           LS++  + L  A+  +FF+W+V  RR++HENPEL +EE ETS+ +R ELE +G+++ +PV
Sbjct: 28  LSQIPSKFLTLAKRNDFFDWMVGIRRRIHENPELGYEEVETSKLVRAELEKMGVSYKYPV 87

Query: 89  AKTGIVASVGSGTHPWFALRADMDALPIQEKVEWEHKSKKDGKMHACGHDVHVTMLLGAA 148
           A TG+V  VG+G  P+ ALRADMDAL +QE VEWEHKSK  GKMHACGHD H TMLLGAA
Sbjct: 88  AVTGVVGYVGTGHAPFVALRADMDALAMQEMVEWEHKSKVPGKMHACGHDAHTTMLLGAA 147

Query: 149 KLLQQRRNELKGTVKLVFQPGEEGRGGAYYMVKEGCVEKVEGIFGLHVAQDMSVGAIGSR 208
           KLL++   EL+GTV LVFQP EEG GGA  +V+ G +E V  IFGLHV   +++G + SR
Sbjct: 148 KLLKEHEEELQGTVVLVFQPAEEGGGGAKKIVEAGVLENVSAIFGLHVTNQLALGQVSSR 207

Query: 209 PGPFTACSGRFLATIQGIGGHAALPHKARDPVLAMSSAIISLQYIISRETDPLDSRVITV 268
            GP  A SG F A I G GGHAALP    DP+LA S+ I+SLQ+++SRE DPLDS+V+TV
Sbjct: 208 EGPMLAGSGFFKAKISGKGGHAALPQHTIDPILAASNVIVSLQHLVSREADPLDSQVVTV 267

Query: 269 GLVKGGETGNVIPETVTFGGTFRSITLEGLYNLKQRIQEVIEFQVGVHQCNATVDFLEEK 328
              +GG   NVIP++VT GGTFR+ + +    LK+RI++VI  Q  V+ CNATVDF+EE+
Sbjct: 268 AKFEGGGAFNVIPDSVTIGGTFRAFSTKSFMQLKKRIEQVITRQASVNMCNATVDFIEEE 327

Query: 329 ARFYPPTINDQSLYNHIEKVGQHLLGGSSNVLHLPNTMGAEDFSFYSQHIPAAFFMIGAK 388
             F+PPT+ND++L+   + V   +L G  N + +   MG+EDFSFY Q IP  F  +G +
Sbjct: 328 KPFFPPTVNDKALHQFFKNVSGDML-GIENYVEMQPLMGSEDFSFYQQAIPGHFSFVGMQ 387

Query: 389 NDTMESGIPLHSPYLVLDEQVLPLGAALHAAVAISYL 426
           N         HSPY  ++E++LP GA+LHA++A  YL
Sbjct: 388 NKARSPMASPHSPYFEVNEELLPYGASLHASMATRYL 423

BLAST of HG10020086 vs. TAIR 10
Match: AT1G51780.1 (IAA-leucine resistant (ILR)-like gene 5 )

HSP 1 Score: 413.7 bits (1062), Expect = 1.8e-115
Identity = 208/398 (52.26%), Postives = 282/398 (70.85%), Query Frame = 0

Query: 28  ELSRLTRELLESARNPEFFEWLVRARRKLHENPELSFEEFETSQFIRTELESLGINFTWP 87
           +LS++ +  L  A+  +FF+W+V  RR++HENPEL +EE ETS+ ++TEL+ +G+++  P
Sbjct: 27  DLSQIPKNFLSLAKREDFFDWMVGIRRRIHENPELGYEEVETSKLVKTELDKMGVSYKNP 86

Query: 88  VAKTGIVASVGSGTHPWFALRADMDALPIQEKVEWEHKSKKDGKMHACGHDVHVTMLLGA 147
           VA TG++  VG+G  P+ ALRADMDALPIQE VEWEHKSK  GKMHACGHD H TMLLGA
Sbjct: 87  VAVTGVIGYVGTGHAPFVALRADMDALPIQEMVEWEHKSKIPGKMHACGHDAHTTMLLGA 146

Query: 148 AKLLQQRRNELKGTVKLVFQPGEEGRGGAYYMVKEGCVEKVEGIFGLHVAQDMSVGAIGS 207
           AKLL++ + EL+GTV LVFQP EEG  GA  +V+ G +E V  IFGLHV+  + +G + S
Sbjct: 147 AKLLKEHQEELQGTVILVFQPAEEGGAGAKKIVEAGVLENVGAIFGLHVSNLLGLGQLSS 206

Query: 208 RPGPFTACSGRFLATIQGIGGHAALPHKARDPVLAMSSAIISLQYIISRETDPLDSRVIT 267
           R G   A SGRF ATI G GGHAALP  A DPVLA S+ I+SLQ+++SRE DPLDS+V+T
Sbjct: 207 REGLLMAGSGRFKATISGKGGHAALPQFAIDPVLAASNVILSLQHLVSREADPLDSQVVT 266

Query: 268 VGLVKGGETGNVIPETVTFGGTFRSITLEGLYNLKQRIQEVIEFQVGVHQCNATVDFLEE 327
           V   +G +  NVIP++VT GGTFR++  +    LKQRI +VI  Q  V+ CNATVDFLE+
Sbjct: 267 VATFEGSDAFNVIPDSVTIGGTFRALLPKSFEQLKQRIVQVITTQASVNMCNATVDFLED 326

Query: 328 KARFYPPTINDQSLYNHIEKVGQHLLGGSSNVLHLPNTMGAEDFSFYSQHIPAAFFMIGA 387
           +   +PPT+N+++L+   + V   +LG  + V  LP  M +EDF+FY Q IP  F  +G 
Sbjct: 327 ETPPFPPTVNNKTLHLFYKNVSVDMLGIENYVETLP-VMVSEDFAFYQQAIPGHFSFVGM 386

Query: 388 KNDTMESGIPLHSPYLVLDEQVLPLGAALHAAVAISYL 426
           +N +       HSP+  ++E++LP GA+L A++A  YL
Sbjct: 387 QNKSHSPMANPHSPFFEVNEELLPYGASLLASLATRYL 423

BLAST of HG10020086 vs. TAIR 10
Match: AT5G56660.1 (IAA-leucine resistant (ILR)-like 2 )

HSP 1 Score: 413.7 bits (1062), Expect = 1.8e-115
Identity = 215/430 (50.00%), Postives = 292/430 (67.91%), Query Frame = 0

Query: 1   MEKLLLCAF---FFIFQFCISSPLETEPPLELSRLTRELLESARNPEFFEWLVRARRKLH 60
           + KLL   F    F+      SP   E   + S++  +LLE A++PE F+W+V+ RRK+H
Sbjct: 3   LNKLLSLTFQLLLFLLSVSSESPWIAE---DTSQIQTKLLEFAKSPEVFDWMVKIRRKIH 62

Query: 61  ENPELSFEEFETSQFIRTELESLGINFTWPVAKTGIVASVGSGTHPWFALRADMDALPIQ 120
           ENPEL +EE ETS+ IR+ELE +GI + +PVA TG++  +G+G  P+ ALRADMDALPIQ
Sbjct: 63  ENPELGYEELETSKLIRSELELIGIKYRYPVAITGVIGYIGTGEPPFVALRADMDALPIQ 122

Query: 121 EKVEWEHKSKKDGKMHACGHDVHVTMLLGAAKLLQQRRNELKGTVKLVFQPGEEGRGGAY 180
           E VEWEHKSK  GKMHACGHD HVTMLLGAAK+L + R+ L+GTV L+FQP EEG  GA 
Sbjct: 123 EGVEWEHKSKIAGKMHACGHDGHVTMLLGAAKILHEHRHHLQGTVVLIFQPAEEGLSGAK 182

Query: 181 YMVKEGCVEKVEGIFGLHVAQDMSVGAIGSRPGPFTACSGRFLATIQGIGGHAALPHKAR 240
            M +EG ++ VE IFG+H++  +  G   SR G F A +G F A I G GGHAA+P    
Sbjct: 183 KMREEGALKNVEAIFGIHLSARIPFGKAASRAGSFLAGAGVFEAVITGKGGHAAIPQHTI 242

Query: 241 DPVLAMSSAIISLQYIISRETDPLDSRVITVGLVKGGETGNVIPETVTFGGTFRSITLEG 300
           DPV+A SS ++SLQ ++SRETDPLDS+V+TV  V GG   NVIP+++T GGT R+ T  G
Sbjct: 243 DPVVAASSIVLSLQQLVSRETDPLDSKVVTVSKVNGGNAFNVIPDSITIGGTLRAFT--G 302

Query: 301 LYNLKQRIQEVIEFQVGVHQCNATVDFLEEKARFYPPTINDQSLYNHIEKVGQHLLGGSS 360
              L+QR++EVI  Q  VH+CNA+V+         PPT+N++ LY   +KV + LLG  +
Sbjct: 303 FTQLQQRVKEVITKQAAVHRCNASVNLTPNGREPMPPTVNNKDLYKQFKKVVRDLLGQEA 362

Query: 361 NVLHLPNTMGAEDFSFYSQHIPAAFFMIGAKNDTMESGIPLHSPYLVLDEQVLPLGAALH 420
            V   P  MG+EDFS++++ IP  F ++G +++T       HSP   ++E VLP GAA+H
Sbjct: 363 FVEAAP-VMGSEDFSYFAETIPGHFSLLGMQDET-NGYASSHSPLYRINEDVLPYGAAIH 422

Query: 421 AAVAISYLDQ 428
           A++A+ YL +
Sbjct: 423 ASMAVQYLKE 425

BLAST of HG10020086 vs. TAIR 10
Match: AT5G56650.1 (IAA-leucine resistant (ILR)-like 1 )

HSP 1 Score: 409.5 bits (1051), Expect = 3.5e-114
Identity = 206/398 (51.76%), Postives = 280/398 (70.35%), Query Frame = 0

Query: 28  ELSRLTRELLESARNPEFFEWLVRARRKLHENPELSFEEFETSQFIRTELESLGINFTWP 87
           ++SR+    LE A++PE F+ +VR RRK+HENPEL +EEFETS+FIR+EL+ +G+ + +P
Sbjct: 29  DVSRIPINFLELAKSPEVFDSMVRIRRKIHENPELGYEEFETSKFIRSELDLIGVKYRFP 88

Query: 88  VAKTGIVASVGSGTHPWFALRADMDALPIQEKVEWEHKSKKDGKMHACGHDVHVTMLLGA 147
           VA TGI+  +G+G  P+ ALRADMDALPIQE VEWEHKSK  GKMHACGHD HV MLLGA
Sbjct: 89  VAITGIIGYIGTGEPPFVALRADMDALPIQEAVEWEHKSKNPGKMHACGHDGHVAMLLGA 148

Query: 148 AKLLQQRRNELKGTVKLVFQPGEEGRGGAYYMVKEGCVEKVEGIFGLHVAQDMSVGAIGS 207
           AK+LQQ R  L+GTV L+FQP EEG  GA  M +EG ++ VE IFG+H++     G   S
Sbjct: 149 AKILQQHRQHLQGTVVLIFQPAEEGLSGAKMMREEGALKNVEAIFGIHLSPRTPFGKAAS 208

Query: 208 RPGPFTACSGRFLATIQGIGGHAALPHKARDPVLAMSSAIISLQYIISRETDPLDSRVIT 267
             G F A +G F A I G GGHAA+P    DPV+A SS ++SLQ+++SRETDP DS+V+T
Sbjct: 209 LAGSFMAGAGAFEAVITGKGGHAAIPQHTIDPVVAASSIVLSLQHLVSRETDPSDSKVVT 268

Query: 268 VGLVKGGETGNVIPETVTFGGTFRSITLEGLYNLKQRIQEVIEFQVGVHQCNATVDFLEE 327
           V  V GG   NVIP+++T GGT R+ T  G   L++RI+E+I  Q  VH+CNA+V+    
Sbjct: 269 VTKVNGGNAFNVIPDSITIGGTLRAFT--GFTQLQERIKEIITKQAAVHRCNASVNLAPN 328

Query: 328 KARFYPPTINDQSLYNHIEKVGQHLLGGSSNVLHLPNTMGAEDFSFYSQHIPAAFFMIGA 387
             +  PPT+N+  LY   +KV + LLG  + V  +P  MG+EDFS++++ IP  F ++G 
Sbjct: 329 GNQPMPPTVNNMDLYKKFKKVVRDLLGQEAFVEAVPE-MGSEDFSYFAETIPGHFSLLGM 388

Query: 388 KNDTMESGIPLHSPYLVLDEQVLPLGAALHAAVAISYL 426
           +++T +     HSP+  ++E VLP GAA+HA +A+ YL
Sbjct: 389 QDET-QGYASSHSPHYRINEDVLPYGAAIHATMAVQYL 422

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004136497.28.1e-22287.59IAA-amino acid hydrolase ILR1-like 3 [Cucumis sativus] >KAE8651096.1 hypothetica... [more]
XP_038906100.13.0e-21688.53IAA-amino acid hydrolase ILR1-like 3 [Benincasa hispida][more]
XP_008442881.13.1e-21384.60PREDICTED: IAA-amino acid hydrolase ILR1-like 3 [Cucumis melo][more]
KAA0043848.16.6e-20886.27IAA-amino acid hydrolase ILR1-like 3 [Cucumis melo var. makuwa] >TYK25287.1 IAA-... [more]
XP_038904781.17.6e-20481.38IAA-amino acid hydrolase ILR1-like 3 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q8H3C93.3e-13358.46IAA-amino acid hydrolase ILR1-like 7 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
P549688.9e-13154.08IAA-amino acid hydrolase ILR1 OS=Arabidopsis thaliana OX=3702 GN=ILR1 PE=1 SV=2[more]
Q851L51.5e-13057.92IAA-amino acid hydrolase ILR1-like 3 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Q8H3C79.2e-12855.69IAA-amino acid hydrolase ILR1-like 9 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Q8H3C82.0e-12752.67IAA-amino acid hydrolase ILR1-like 8 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Match NameE-valueIdentityDescription
A0A0A0LGQ73.9e-22287.59M20_dimer domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G778230 P... [more]
A0A1S3B6R31.5e-21384.60IAA-amino acid hydrolase ILR1-like 3 OS=Cucumis melo OX=3656 GN=LOC103486646 PE=... [more]
A0A5A7TLW93.2e-20886.27IAA-amino acid hydrolase ILR1-like 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A0A0LEN61.7e-20180.46M20_dimer domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G778240 P... [more]
A0A6J1KSE71.3e-19678.44IAA-amino acid hydrolase ILR1-like 3 OS=Cucurbita maxima OX=3661 GN=LOC111498280... [more]
Match NameE-valueIdentityDescription
AT3G02875.16.3e-13254.08Peptidase M20/M25/M40 family protein [more]
AT1G51760.18.6e-12152.90peptidase M20/M25/M40 family protein [more]
AT1G51780.11.8e-11552.26IAA-leucine resistant (ILR)-like gene 5 [more]
AT5G56660.11.8e-11550.00IAA-leucine resistant (ILR)-like 2 [more]
AT5G56650.13.5e-11451.76IAA-leucine resistant (ILR)-like 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR017439AmidohydrolaseTIGRFAMTIGR01891TIGR01891coord: 50..413
e-value: 1.3E-113
score: 377.8
IPR017439AmidohydrolasePIRSFPIRSF005962Amidohydrol_AmhXcoord: 35..428
e-value: 5.1E-121
score: 401.9
IPR017439AmidohydrolasePANTHERPTHR11014PEPTIDASE M20 FAMILY MEMBERcoord: 11..429
IPR002933Peptidase M20PFAMPF01546Peptidase_M20coord: 106..418
e-value: 7.7E-34
score: 117.6
NoneNo IPR availableGENE3D3.30.70.360coord: 214..332
e-value: 4.3E-133
score: 445.6
NoneNo IPR availableGENE3D3.40.630.10Zn peptidasescoord: 50..410
e-value: 4.3E-133
score: 445.6
NoneNo IPR availablePANTHERPTHR11014:SF108IAA-AMINO ACID HYDROLASE ILR1coord: 11..429
NoneNo IPR availableSUPERFAMILY53187Zn-dependent exopeptidasescoord: 44..425
IPR011650Peptidase M20, dimerisation domainPFAMPF07687M20_dimercoord: 221..310
e-value: 3.8E-7
score: 30.0
IPR044757IAA-amino acid hydrolase ILR1-likeCDDcd08017M20_IAA_Hydcoord: 49..425
e-value: 0.0
score: 618.565
IPR036264Bacterial exopeptidase dimerisation domainSUPERFAMILY55031Bacterial exopeptidase dimerisation domaincoord: 216..333

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10020086.1HG10020086.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009850 auxin metabolic process
molecular_function GO:0010179 IAA-Ala conjugate hydrolase activity
molecular_function GO:0016787 hydrolase activity