HG10016194 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10016194
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionLactoylglutathione lyase
LocationChr03: 3441152 .. 3445933 (-)
RNA-Seq ExpressionHG10016194
SyntenyHG10016194
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGAGGCTGCATCTCTTGCTCTGAATGCCGAGTTGTTGGAATGGCCCAAGAAAGACAAGCGTCGGTTTCTTCATGTTGTGTACCGTGTTGGTGACCTTGACCGCACTATCAAGTATGATTTTATTATCTAGCGCAACCACAACCAGTTTGCATTTTGCATTTGATTGGTCTGATTATTTGTTTACTTTGTTGTTTCTTGCTTTTCAGGTTTTATACTGAATGTTTTGGGATGAAATTATTAAGGAAGAGGGATGTGCCAGAAGAGAACTATTCTAATGCTTTCCTTGGATTTGGGCCAGAGGATTCTAATTTTGTGGTGGAGTTGACATATAGTATGTGCCCTACATTTATATGTTTCAACTTGCTCTTGCATATCTCTGATTATGTTCTCAGCTTAAACGTGAAGTACTCCTGATTGGAGTCAATTCGTAATATAGATAAAATCTCAACAAAATATCTATCTTCAACAATATTTTGTCTGTCGGTTCCTACCCATCAAAATTGTGGGGTGGAAAACTTAAAATTATGGCCTTAGCTTATTTTCCTTGAATACTTGGCAAACACCATTGGTTATGTAGTATGTTTTGTTCATCTGTCTTCCACGATATTTTGTCTGCCGGTTTCTACCCATGAAAGTTATACGGTCAGGGGAGAAATGAATATGTTTCTTCCCTTGCCTAGTCGCTTAAGATCGAGCATTAATGGGTTGATCTAAATTCTGAAATGTTTCTTTTCCAGAGTTCGTTACAACTTATCTATTTTGGTTGGGTGATAAACTTATATGTTTAAGGTAGGTTATATTGATGGTGTTTTGCAGATTATGGAGTTAGTTCTTATGATATTGGGACAGGCTTTGGCCATTTTGCTATCGCAACTCAAGATGTAAGAGTGTCTCATTTTAGCAAGTTCACTTGTTTGCATTCAACTGAAAATAAACTCTTGTGAACTTCTAGGTGTATAAACTTGTAGAAGATATACGAGCCAAGGGTGGAAGCATTACTAGGGAGCCTGGTCCTGTCAAAGGAGGATCCTCTGTTATTGCCTTTGCAAAGGATCCCGATGGTTATATTTTTGAACTCATCCAAAGAGGTCCTACTCCTGAGCCACTCTGTCAAGTAATGCTTCGTGTTGGTGATCTAGAACGCTCTATCAAGTTTTATGAGAAGGTATCTCATTGAAAGAATTTGTTTGTATCTGTTCCCTTTAAATATCCTACTGTTTTATTGAAGAAATTGTTTCATAGAGGGGAGTCCTTGAACGATGATGTGTATCTGATTAAAAGCTGGTAATTATGATTATTGTGTCTTCTATGTTTTAACCTTCGAGCAATATGATTTAGGAAAATTAATGATTGTAGAAAGCCTTGCTCAAGTGTGCAGATCGTAAAATAAAGTGTTATGAGATTCATTAGTGATAAGAGTACATTTCAGTGAAAAGCTTTAGAAATAATCTTTCCTTGTTAACATAAGTTGTTTGAGATGTATATTCAAATTTCCATTCTATCAGATTCTGTTCTCGTATTTTAAGGTTTTTCTTTGCGTAGGCCTTGGGAATGAGGCTATTGAAGAAGGTTGATAGGCCAGAATACAAGGTTAGCTAATTTTCTTTTAGTAGTTACCACCAATTCTTTCACTACGGATTTATCTCTAGACTTTTTTATGCTGAAAATGCTTTTGGATCCTTTTATCTTGCTATATGCATCTGACAGTATACCCTAGCCATGATGGGATATGCTGATGAAGTAGAGACAACAGTGCTAGAGTTGACTTATAATTATGGTGTGACAGAGTATACCAAGGGAAATGCATATGCTCAGGTGAATTTATTGTATACCTCTCCCCATCTATTATTTATTTATTTTAAATGGGAACTCTTCTTCTTTCCATTGTCTATTTGTTAAACGATTATTTTGGTATGTTTCAGGTTGCCATAAGTACTGATGATGTATACAAGAGTGCTGAGGTTGTCAACCTGGTTAATCAGGAACTTGGAGGAAAGATTACTCGACAACCAGGACCACTTCCTGGAATCGGCACAAAAATTGTTTCCTTTCTGGATCCTGATGGTTGGAAGACAGTAAGTACCTTATTCACGTATCACTTTAAAAATACTTCTCACTATTTTTATAATTTTTAAAAAGGAAAATTTTCTTTTTAGTCTATGAGTTTTAAAGAATAGGTGCGTTTGGTCCCTAAGGCTTCAAATTGGCCATTTTACTTCCTAAGTTTTAATGAATTGGTTTAAAAGGTCTCTCCTATAGTAATTAGGTTTTTTTTATTATATTTAATATTGATATTACATTTTTAAAATAATTAAAAAAAGAAATGTTTTCTTTCACCTCTTTCTTCCCATTCTCTCTCCTACCTCCTTTCCATCTTCTCATATTCCTCACCTCTTCAATCCCACAGGCTATTTCCCTCCCATTCTTACTCTTATTCAAATTAAAATGCTGCTCTTACTTCAATTCTCCCTGTTTTCAGAGCCCAGATTTAGGTGAATAAGTGTGAATGATCATCATTAACTATATTTCTTGGAACTCTTCTCTCCCCTTCCCTCATAACCTTTCAGCTCCATCCCTCCCTCATCCTTCAGCCCTCTCTTCTGATCCCATCAACCCCACAGCTCCCTTGGTTAAACCTCCTATACCGCCCTTTCTTCCTTCTTCACCGCCGGAAACCACAGCACCATCTCCTTCATCTCTCCCATCCGAACTCATTAACTCCCCTGCTCTCACATCCAAGCAACCTACCCTTCCCCCTCATTCTACTTCACCTTTGACTTCCACAATCCCTACCCCCTCACCACCTTCTATTTACCCAACCACAAAGAACGTGGCATCTTCCTCAACCTTCACCAACAACAGTGTTTCAAAAGACTTTTACCCTTCACCATTTTTGGATTATCCAACCATTCGCAACCCGGGATCTTCCTCAAACATCAACAATAGCTGCGTGCCTCCCTCTACTACCATGGAAACTGTTTTTCGTCCCGATATTAAACATTCTACTGCCATCATTGCTCTTCCATCCGTTGCCGATTCAGAAACCTTTCTTTCTAGCCCCATGCCTTATTCACCTCAATCTCCACATGAACATTCTGGAGAAGATTGGTATGATTTTTACCCACCAACAAGTTTTAAATCTTTCAATCCCGCTCCTCATTTGATGGCCTTGGCTTCATGGTTTAGAGAACTTGGCCTATGCATAAAGGCAATCCCATCTTGTAAACAATCCAACCAGCTATCAACTAAGAAAAAAAGGTGCCGAGAGAACTTCGAAATCTTAAAAGCTCTATATCATATGACAAAAAAGCGGCAGATTTGATCGGAAAAGGCCGTCATCAATATGTTTCTGCAGATTGATTTCCTCCTTGACTTTTTTGTTCTTTCTGAAATGGGGTGTTGGGGACCTGATTATTGGGCCATCTTTCATCAGTGGTGATCTACATGAAGCTTGGCCTTTTTCATTTTAGATGTGCTTCATTGTGTCTCCTTGCTCCATTTTTTTTTGGGGTCTGCTGCTTTTGTTTCTTTCCATCTCATTTGGTGGTTCATCTTCTATCTTACCCTCTCGTATGGGCTTCTTTTATATTTTGGTGACTCTCCATATTGTATCCTTTGGGCTATATTTCATTTATCAATGAAATGTTTCTTATCTAAAAAAAAAGAAAACTATATTTCTTGGAGCTCAAATCCAAGGTATAGAGCATCCAATACGTGGGACGCCAATCCACCCCACAATCCGCCATCAAAACTGCTAGAGCTCAAATCAAAGGGACGATGAGGATCAAAACCACTATTGTAAGTTTGAGGGTCCAATTTTAACACTTGCAGAAGTTCGAGGGCCCAATTTTTAAAATTAAAAGTTTGAGAGTATAATTGCAACTACCACCATACTTTAGGGGTGGTTTTTGTAATTTTTCCTAAATTTAATGAAAAATACCTAATTATTGTAGCAAAGACTTTTTAAACCTATTTATTAAAATTCAAGGACTAAAATGACCAATTTGAAACTTTAGGAACCAAATGCACCTATTCTTTAAAACTCATAGACTGAAAAGGTGCTTTACCATTTTTAAAATAATGGTTCTACATTAAAAAAAAAATGGGATGTGTCTGTGTGGAACAAGATTGTTCGTTACAAGAAAATCCAAGTCGGATTACCTCTAGTGGCTGCTTTCAATGTTACACATGTTTAGTGTCATGAGATTAACTTCAAGAATGTTTTGGAAAAAAGGATCATTTGGGATAGTTGAAAGAGATATATTAGTTAGTGTCTTGATATATTAGCTCGCTTGGTGTGTTCGATCTTATGGATCTTGTGGGGTGAGCAGAATAATAGGACTTTCAGGGGTGGATAGAGACCCAAGTGAAGTTTCGTCTCTTGTTTGCTTTCATGTTTATTTGTGAGCTTCAATTTCGAAGACCTCTTGTAACTATTCTGTAGGCGTTATTTTGCATAGATGGAGTCTCTTCTTGCAGAGGGATGTTTCTTTTTGTGTGTGTTCTTTCATATTTTTTTCTCAATGAAAGTAGTTGCTTCGATAAAAAAAAAGGTTTCCTAATAGTGGACCTCTGGACGGTTTTGACATAAATTGGTGCCAAACATATGATAGTGCAAGTCATGCACTCAACTTAGAATTTGAGTTATATATTACATCTGATCTTAATCCTACTTGTTTGTTAGCTGATTTCTGAATTTACTTTTGTAAGCTTTTCATTATTCTTGTAGGTCCTTGTCGATAATGCAGACTTCCAAAAGGAATTGGAACATCAATGA

mRNA sequence

ATGGCCGAGGCTGCATCTCTTGCTCTGAATGCCGAGTTGTTGGAATGGCCCAAGAAAGACAAGCGTCGGTTTCTTCATGTTGTGTACCGTGTTGGTGACCTTGACCGCACTATCAAGTTTTATACTGAATGTTTTGGGATGAAATTATTAAGGAAGAGGGATGTGCCAGAAGAGAACTATTCTAATGCTTTCCTTGGATTTGGGCCAGAGGATTCTAATTTTGTGGTGGAGTTGACATATAATTATGGAGTTAGTTCTTATGATATTGGGACAGGCTTTGGCCATTTTGCTATCGCAACTCAAGATGTGTATAAACTTGTAGAAGATATACGAGCCAAGGGTGGAAGCATTACTAGGGAGCCTGGTCCTGTCAAAGGAGGATCCTCTGTTATTGCCTTTGCAAAGGATCCCGATGGTTATATTTTTGAACTCATCCAAAGAGGTCCTACTCCTGAGCCACTCTGTCAAGTAATGCTTCGTGTTGGTGATCTAGAACGCTCTATCAAGTTTTATGAGAAGGCCTTGGGAATGAGGCTATTGAAGAAGGTTGATAGGCCAGAATACAAGTATACCCTAGCCATGATGGGATATGCTGATGAAGTAGAGACAACAGTGCTAGAGTTGACTTATAATTATGGTGTGACAGAGTATACCAAGGGAAATGCATATGCTCAGGTTGCCATAAGTACTGATGATGTATACAAGAGTGCTGAGGTTGTCAACCTGGTTAATCAGGAACTTGGAGGAAAGATTACTCGACAACCAGGACCACTTCCTGGAATCGGCACAAAAATTGTTTCCTTTCTGGATCCTGATGGTTGGAAGACAGTCCTTGTCGATAATGCAGACTTCCAAAAGGAATTGGAACATCAATGA

Coding sequence (CDS)

ATGGCCGAGGCTGCATCTCTTGCTCTGAATGCCGAGTTGTTGGAATGGCCCAAGAAAGACAAGCGTCGGTTTCTTCATGTTGTGTACCGTGTTGGTGACCTTGACCGCACTATCAAGTTTTATACTGAATGTTTTGGGATGAAATTATTAAGGAAGAGGGATGTGCCAGAAGAGAACTATTCTAATGCTTTCCTTGGATTTGGGCCAGAGGATTCTAATTTTGTGGTGGAGTTGACATATAATTATGGAGTTAGTTCTTATGATATTGGGACAGGCTTTGGCCATTTTGCTATCGCAACTCAAGATGTGTATAAACTTGTAGAAGATATACGAGCCAAGGGTGGAAGCATTACTAGGGAGCCTGGTCCTGTCAAAGGAGGATCCTCTGTTATTGCCTTTGCAAAGGATCCCGATGGTTATATTTTTGAACTCATCCAAAGAGGTCCTACTCCTGAGCCACTCTGTCAAGTAATGCTTCGTGTTGGTGATCTAGAACGCTCTATCAAGTTTTATGAGAAGGCCTTGGGAATGAGGCTATTGAAGAAGGTTGATAGGCCAGAATACAAGTATACCCTAGCCATGATGGGATATGCTGATGAAGTAGAGACAACAGTGCTAGAGTTGACTTATAATTATGGTGTGACAGAGTATACCAAGGGAAATGCATATGCTCAGGTTGCCATAAGTACTGATGATGTATACAAGAGTGCTGAGGTTGTCAACCTGGTTAATCAGGAACTTGGAGGAAAGATTACTCGACAACCAGGACCACTTCCTGGAATCGGCACAAAAATTGTTTCCTTTCTGGATCCTGATGGTTGGAAGACAGTCCTTGTCGATAATGCAGACTTCCAAAAGGAATTGGAACATCAATGA

Protein sequence

MAEAASLALNAELLEWPKKDKRRFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEENYSNAFLGFGPEDSNFVVELTYNYGVSSYDIGTGFGHFAIATQDVYKLVEDIRAKGGSITREPGPVKGGSSVIAFAKDPDGYIFELIQRGPTPEPLCQVMLRVGDLERSIKFYEKALGMRLLKKVDRPEYKYTLAMMGYADEVETTVLELTYNYGVTEYTKGNAYAQVAISTDDVYKSAEVVNLVNQELGGKITRQPGPLPGIGTKIVSFLDPDGWKTVLVDNADFQKELEHQ
Homology
BLAST of HG10016194 vs. NCBI nr
Match: XP_038882184.1 (lactoylglutathione lyase GLX1 [Benincasa hispida] >XP_038882185.1 lactoylglutathione lyase GLX1 [Benincasa hispida] >XP_038882186.1 lactoylglutathione lyase GLX1 [Benincasa hispida])

HSP 1 Score: 586.6 bits (1511), Expect = 1.1e-163
Identity = 287/291 (98.63%), Postives = 289/291 (99.31%), Query Frame = 0

Query: 1   MAEAASLALNAELLEWPKKDKRRFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEENY 60
           MAEAASLA NAELLEWPKKDKRRFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEE Y
Sbjct: 1   MAEAASLAPNAELLEWPKKDKRRFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKY 60

Query: 61  SNAFLGFGPEDSNFVVELTYNYGVSSYDIGTGFGHFAIATQDVYKLVEDIRAKGGSITRE 120
           SNAFLGFGPEDSNFVVELTYNYG+SSYDIGTGFGHFAIATQDVYKLVEDIRAKGGSITRE
Sbjct: 61  SNAFLGFGPEDSNFVVELTYNYGISSYDIGTGFGHFAIATQDVYKLVEDIRAKGGSITRE 120

Query: 121 PGPVKGGSSVIAFAKDPDGYIFELIQRGPTPEPLCQVMLRVGDLERSIKFYEKALGMRLL 180
           PGPVKGGSSVIAFAKDPDGYIFELIQRGPTPEPLCQVMLRVGDLERSIKFYEKALGMRLL
Sbjct: 121 PGPVKGGSSVIAFAKDPDGYIFELIQRGPTPEPLCQVMLRVGDLERSIKFYEKALGMRLL 180

Query: 181 KKVDRPEYKYTLAMMGYADEVETTVLELTYNYGVTEYTKGNAYAQVAISTDDVYKSAEVV 240
           KKVDRPEYKYTLAMMGYADE+ETTVLELTYNYGVTEYTKGNAYAQVAISTDDVYKSAEVV
Sbjct: 181 KKVDRPEYKYTLAMMGYADELETTVLELTYNYGVTEYTKGNAYAQVAISTDDVYKSAEVV 240

Query: 241 NLVNQELGGKITRQPGPLPGIGTKIVSFLDPDGWKTVLVDNADFQKELEHQ 292
           NLVNQELGGKITRQPGPLPGIGTKIVSFLDPDGWKTVLVDNADFQKELEHQ
Sbjct: 241 NLVNQELGGKITRQPGPLPGIGTKIVSFLDPDGWKTVLVDNADFQKELEHQ 291

BLAST of HG10016194 vs. NCBI nr
Match: KAG6603781.1 (Lactoylglutathione lyase GLX1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 577.0 bits (1486), Expect = 9.1e-161
Identity = 282/291 (96.91%), Postives = 286/291 (98.28%), Query Frame = 0

Query: 1   MAEAASLALNAELLEWPKKDKRRFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEENY 60
           MAEAASLA NA+LLEWPKKDKRRFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEE Y
Sbjct: 1   MAEAASLAPNADLLEWPKKDKRRFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKY 60

Query: 61  SNAFLGFGPEDSNFVVELTYNYGVSSYDIGTGFGHFAIATQDVYKLVEDIRAKGGSITRE 120
           SNAFLGFGPEDSNFVVELTYNYGVSSYDIGTGFGHFAIA+QDVYKLVEDIRAKGGSITRE
Sbjct: 61  SNAFLGFGPEDSNFVVELTYNYGVSSYDIGTGFGHFAIASQDVYKLVEDIRAKGGSITRE 120

Query: 121 PGPVKGGSSVIAFAKDPDGYIFELIQRGPTPEPLCQVMLRVGDLERSIKFYEKALGMRLL 180
           PGPVKGGSSVIAFAKDPDGYIFELIQRGPTPEPLCQVMLRVGDLERSIKFYEKALGMRLL
Sbjct: 121 PGPVKGGSSVIAFAKDPDGYIFELIQRGPTPEPLCQVMLRVGDLERSIKFYEKALGMRLL 180

Query: 181 KKVDRPEYKYTLAMMGYADEVETTVLELTYNYGVTEYTKGNAYAQVAISTDDVYKSAEVV 240
           KKVDRPEYKYTLAMMGYADE+ETTVLELTYNYGVTEYTKGNAYAQVAI TDDVYKSAEV+
Sbjct: 181 KKVDRPEYKYTLAMMGYADELETTVLELTYNYGVTEYTKGNAYAQVAIGTDDVYKSAEVI 240

Query: 241 NLVNQELGGKITRQPGPLPGIGTKIVSFLDPDGWKTVLVDNADFQKELEHQ 292
            LVNQELGGKITRQ GPLPGIGTKIVSFLDPDGWKTVLVDNADFQKELEHQ
Sbjct: 241 KLVNQELGGKITRQAGPLPGIGTKIVSFLDPDGWKTVLVDNADFQKELEHQ 291

BLAST of HG10016194 vs. NCBI nr
Match: KAG7033953.1 (hypothetical protein SDJN02_03679 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 576.6 bits (1485), Expect = 1.2e-160
Identity = 281/291 (96.56%), Postives = 286/291 (98.28%), Query Frame = 0

Query: 1   MAEAASLALNAELLEWPKKDKRRFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEENY 60
           MAEAASLA NA+LLEWPKKDKRRFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEE Y
Sbjct: 31  MAEAASLAPNADLLEWPKKDKRRFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKY 90

Query: 61  SNAFLGFGPEDSNFVVELTYNYGVSSYDIGTGFGHFAIATQDVYKLVEDIRAKGGSITRE 120
           SNAFLGFGPEDSNFVVELTYNYG+SSYDIGTGFGHFAIA+QDVYKLVEDIRAKGGSITRE
Sbjct: 91  SNAFLGFGPEDSNFVVELTYNYGISSYDIGTGFGHFAIASQDVYKLVEDIRAKGGSITRE 150

Query: 121 PGPVKGGSSVIAFAKDPDGYIFELIQRGPTPEPLCQVMLRVGDLERSIKFYEKALGMRLL 180
           PGPVKGGSSVIAFAKDPDGYIFELIQRGPTPEPLCQVMLRVGDLERSIKFYEKALGMRLL
Sbjct: 151 PGPVKGGSSVIAFAKDPDGYIFELIQRGPTPEPLCQVMLRVGDLERSIKFYEKALGMRLL 210

Query: 181 KKVDRPEYKYTLAMMGYADEVETTVLELTYNYGVTEYTKGNAYAQVAISTDDVYKSAEVV 240
           KKVDRPEYKYTLAMMGYADE+ETTVLELTYNYGVTEYTKGNAYAQVAI TDDVYKSAEV+
Sbjct: 211 KKVDRPEYKYTLAMMGYADELETTVLELTYNYGVTEYTKGNAYAQVAIGTDDVYKSAEVI 270

Query: 241 NLVNQELGGKITRQPGPLPGIGTKIVSFLDPDGWKTVLVDNADFQKELEHQ 292
            LVNQELGGKITRQ GPLPGIGTKIVSFLDPDGWKTVLVDNADFQKELEHQ
Sbjct: 271 KLVNQELGGKITRQAGPLPGIGTKIVSFLDPDGWKTVLVDNADFQKELEHQ 321

BLAST of HG10016194 vs. NCBI nr
Match: XP_023543716.1 (lactoylglutathione lyase GLX1 [Cucurbita pepo subsp. pepo] >XP_023543718.1 lactoylglutathione lyase GLX1 [Cucurbita pepo subsp. pepo] >XP_023543719.1 lactoylglutathione lyase GLX1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 575.5 bits (1482), Expect = 2.6e-160
Identity = 281/291 (96.56%), Postives = 285/291 (97.94%), Query Frame = 0

Query: 1   MAEAASLALNAELLEWPKKDKRRFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEENY 60
           MAE ASLA NA+LLEWPKKDKRRFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEE Y
Sbjct: 1   MAEVASLAPNADLLEWPKKDKRRFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKY 60

Query: 61  SNAFLGFGPEDSNFVVELTYNYGVSSYDIGTGFGHFAIATQDVYKLVEDIRAKGGSITRE 120
           SNAFLGFGPEDSNFVVELTYNYGVSSYDIGTGFGHFAIA+QDVYKLVEDIRAKGGSITRE
Sbjct: 61  SNAFLGFGPEDSNFVVELTYNYGVSSYDIGTGFGHFAIASQDVYKLVEDIRAKGGSITRE 120

Query: 121 PGPVKGGSSVIAFAKDPDGYIFELIQRGPTPEPLCQVMLRVGDLERSIKFYEKALGMRLL 180
           PGPVKGGSSVIAFAKDPDGYIFELIQRGPTPEPLCQVMLRVGDLERSIKFYEKALGMRLL
Sbjct: 121 PGPVKGGSSVIAFAKDPDGYIFELIQRGPTPEPLCQVMLRVGDLERSIKFYEKALGMRLL 180

Query: 181 KKVDRPEYKYTLAMMGYADEVETTVLELTYNYGVTEYTKGNAYAQVAISTDDVYKSAEVV 240
           KKVDRPEYKYTLAMMGYADE+ETTVLELTYNYGVTEYTKGNAYAQVAI TDDVYKSAEV+
Sbjct: 181 KKVDRPEYKYTLAMMGYADELETTVLELTYNYGVTEYTKGNAYAQVAIGTDDVYKSAEVI 240

Query: 241 NLVNQELGGKITRQPGPLPGIGTKIVSFLDPDGWKTVLVDNADFQKELEHQ 292
            LVNQELGGKITRQ GPLPGIGTKIVSFLDPDGWKTVLVDNADFQKELEHQ
Sbjct: 241 KLVNQELGGKITRQAGPLPGIGTKIVSFLDPDGWKTVLVDNADFQKELEHQ 291

BLAST of HG10016194 vs. NCBI nr
Match: XP_022950060.1 (lactoylglutathione lyase GLX1 [Cucurbita moschata] >XP_022950061.1 lactoylglutathione lyase GLX1 [Cucurbita moschata] >XP_022950062.1 lactoylglutathione lyase GLX1 [Cucurbita moschata])

HSP 1 Score: 575.5 bits (1482), Expect = 2.6e-160
Identity = 280/291 (96.22%), Postives = 286/291 (98.28%), Query Frame = 0

Query: 1   MAEAASLALNAELLEWPKKDKRRFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEENY 60
           MAEAASLA NA+LLEWPKKD+RRFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEE Y
Sbjct: 1   MAEAASLAPNADLLEWPKKDRRRFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKY 60

Query: 61  SNAFLGFGPEDSNFVVELTYNYGVSSYDIGTGFGHFAIATQDVYKLVEDIRAKGGSITRE 120
           SNAFLGFGPEDSNFVVELTYNYG+SSYDIGTGFGHFAIA+QDVYKLVEDIRAKGGSITRE
Sbjct: 61  SNAFLGFGPEDSNFVVELTYNYGISSYDIGTGFGHFAIASQDVYKLVEDIRAKGGSITRE 120

Query: 121 PGPVKGGSSVIAFAKDPDGYIFELIQRGPTPEPLCQVMLRVGDLERSIKFYEKALGMRLL 180
           PGPVKGGSSVIAFAKDPDGYIFELIQRGPTPEPLCQVMLRVGDLERSIKFYEKALGMRLL
Sbjct: 121 PGPVKGGSSVIAFAKDPDGYIFELIQRGPTPEPLCQVMLRVGDLERSIKFYEKALGMRLL 180

Query: 181 KKVDRPEYKYTLAMMGYADEVETTVLELTYNYGVTEYTKGNAYAQVAISTDDVYKSAEVV 240
           KKVDRPEYKYTLAMMGYADE+ETTVLELTYNYGVTEYTKGNAYAQVAI TDDVYKSAEV+
Sbjct: 181 KKVDRPEYKYTLAMMGYADELETTVLELTYNYGVTEYTKGNAYAQVAIGTDDVYKSAEVI 240

Query: 241 NLVNQELGGKITRQPGPLPGIGTKIVSFLDPDGWKTVLVDNADFQKELEHQ 292
            LVNQELGGKITRQ GPLPGIGTKIVSFLDPDGWKTVLVDNADFQKELEHQ
Sbjct: 241 KLVNQELGGKITRQAGPLPGIGTKIVSFLDPDGWKTVLVDNADFQKELEHQ 291

BLAST of HG10016194 vs. ExPASy Swiss-Prot
Match: O65398 (Lactoylglutathione lyase GLX1 OS=Arabidopsis thaliana OX=3702 GN=GLX1 PE=1 SV=1)

HSP 1 Score: 507.7 bits (1306), Expect = 8.8e-143
Identity = 239/283 (84.45%), Postives = 262/283 (92.58%), Query Frame = 0

Query: 7   LALNAELLEWPKKDKRRFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEENYSNAFLG 66
           +A  ++LLEWPKKD RRFLHVVYRVGDLDRTI+FYTE FGMKLLRKRD+PEE YSNAFLG
Sbjct: 1   MAEASDLLEWPKKDNRRFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPEEKYSNAFLG 60

Query: 67  FGPEDSNFVVELTYNYGVSSYDIGTGFGHFAIATQDVYKLVEDIRAKGGSITREPGPVKG 126
           FGPE SNFVVELTYNYGVSSYDIGTGFGHFAI+TQDV KLVE++RAKGG++TREPGPVKG
Sbjct: 61  FGPETSNFVVELTYNYGVSSYDIGTGFGHFAISTQDVSKLVENVRAKGGNVTREPGPVKG 120

Query: 127 GSSVIAFAKDPDGYIFELIQRGPTPEPLCQVMLRVGDLERSIKFYEKALGMRLLKKVDRP 186
           G SVIAF KDPDGY FELIQRGPTPEP CQVMLRVGDL+R+IKFYEKALGMRLL+K++RP
Sbjct: 121 GGSVIAFVKDPDGYTFELIQRGPTPEPFCQVMLRVGDLDRAIKFYEKALGMRLLRKIERP 180

Query: 187 EYKYTLAMMGYADEVETTVLELTYNYGVTEYTKGNAYAQVAISTDDVYKSAEVVNLVNQE 246
           EYKYT+ MMGYA+E E+ VLELTYNY VTEYTKGNAYAQ+AI TDDVYKS EV+ +VNQE
Sbjct: 181 EYKYTIGMMGYAEEYESIVLELTYNYDVTEYTKGNAYAQIAIGTDDVYKSGEVIKIVNQE 240

Query: 247 LGGKITRQPGPLPGIGTKIVSFLDPDGWKTVLVDNADFQKELE 290
           LGGKITR+ GPLPG+GTKIVSFLDPDGWKTVLVDN DF KELE
Sbjct: 241 LGGKITREAGPLPGLGTKIVSFLDPDGWKTVLVDNKDFLKELE 283

BLAST of HG10016194 vs. ExPASy Swiss-Prot
Match: Q39366 (Putative lactoylglutathione lyase OS=Brassica oleracea var. gemmifera OX=178616 PE=2 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 9.8e-142
Identity = 239/283 (84.45%), Postives = 263/283 (92.93%), Query Frame = 0

Query: 7   LALNAELLEWPKKDKRRFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEENYSNAFLG 66
           +A NA+L+EWPKKDKRRFLHVVYRVGDLDRTI+FYTECFGMK+LRKRDVPEE YSNAFLG
Sbjct: 1   MAENADLVEWPKKDKRRFLHVVYRVGDLDRTIQFYTECFGMKVLRKRDVPEEKYSNAFLG 60

Query: 67  FGPEDSNFVVELTYNYGVSSYDIGTGFGHFAIATQDVYKLVEDIRAKGGSITREPGPVKG 126
           FGPE SNFVVELTYNYGVSSYDIGTGFGHFAI+TQDV K+VE +RAKGG++TREPGPVKG
Sbjct: 61  FGPETSNFVVELTYNYGVSSYDIGTGFGHFAISTQDVSKMVEAVRAKGGNVTREPGPVKG 120

Query: 127 GSSVIAFAKDPDGYIFELIQRGPTPEPLCQVMLRVGDLERSIKFYEKALGMRLLKKVDRP 186
           G SVIAF KDPDGY FELIQRGPTPEPLCQVMLRVGDL+R++KF EKALGMRLL++++RP
Sbjct: 121 GGSVIAFVKDPDGYTFELIQRGPTPEPLCQVMLRVGDLDRAVKFMEKALGMRLLRRIERP 180

Query: 187 EYKYTLAMMGYADEVETTVLELTYNYGVTEYTKGNAYAQVAISTDDVYKSAEVVNLVNQE 246
           EY  T+ MMGYA+E E+ VLELTYNYGVTEYTKGNAYAQ+AI TDDVYKSAEVV +VNQE
Sbjct: 181 EYN-TIGMMGYAEEYESIVLELTYNYGVTEYTKGNAYAQIAIGTDDVYKSAEVVKIVNQE 240

Query: 247 LGGKITRQPGPLPGIGTKIVSFLDPDGWKTVLVDNADFQKELE 290
           LGGKITR+ GPLPG+GTKIVSFLDPDGWK VLVDN DF KELE
Sbjct: 241 LGGKITREAGPLPGLGTKIVSFLDPDGWKQVLVDNEDFLKELE 282

BLAST of HG10016194 vs. ExPASy Swiss-Prot
Match: Q948T6 (Lactoylglutathione lyase OS=Oryza sativa subsp. japonica OX=39947 GN=GLYI-11 PE=1 SV=2)

HSP 1 Score: 479.6 bits (1233), Expect = 2.6e-134
Identity = 228/278 (82.01%), Postives = 251/278 (90.29%), Query Frame = 0

Query: 13  LLEWPKKDKRRFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEENYSNAFLGFGPEDS 72
           +LEWPKKDK+R LH VYRVGDLDRTIK YTECFGMKLLRKRDVPEE Y+NAFLGFGPED+
Sbjct: 14  VLEWPKKDKKRLLHAVYRVGDLDRTIKCYTECFGMKLLRKRDVPEEKYTNAFLGFGPEDT 73

Query: 73  NFVVELTYNYGVSSYDIGTGFGHFAIATQDVYKLVEDIRAK-GGSITREPGPVKGGSSVI 132
           NF +ELTYNYGV  YDIG GFGHFAIAT+DVYKL E I++     ITREPGPVKGGS+VI
Sbjct: 74  NFALELTYNYGVDKYDIGAGFGHFAIATEDVYKLAEKIKSSCCCKITREPGPVKGGSTVI 133

Query: 133 AFAKDPDGYIFELIQRGPTPEPLCQVMLRVGDLERSIKFYEKALGMRLLKKVDRPEYKYT 192
           AFA+DPDGY+FELIQRGPTPEPLCQVMLRVGDL+RSIKFYEKALGM+LL+K D P+YKYT
Sbjct: 134 AFAQDPDGYMFELIQRGPTPEPLCQVMLRVGDLDRSIKFYEKALGMKLLRKKDVPDYKYT 193

Query: 193 LAMMGYADEVETTVLELTYNYGVTEYTKGNAYAQVAISTDDVYKSAEVVNLVNQELGGKI 252
           +AM+GYADE +TTV+ELTYNYGVTEYTKGNAYAQVAI T+DVYKSAE V LV +ELGGKI
Sbjct: 194 IAMLGYADEDKTTVIELTYNYGVTEYTKGNAYAQVAIGTEDVYKSAEAVELVTKELGGKI 253

Query: 253 TRQPGPLPGIGTKIVSFLDPDGWKTVLVDNADFQKELE 290
            RQPGPLPG+ TKI SFLDPDGWK VLVDNADF KEL+
Sbjct: 254 LRQPGPLPGLNTKIASFLDPDGWKVVLVDNADFLKELQ 291

BLAST of HG10016194 vs. ExPASy Swiss-Prot
Match: Q8W593 (Probable lactoylglutathione lyase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g67280 PE=1 SV=1)

HSP 1 Score: 444.9 bits (1143), Expect = 7.0e-124
Identity = 210/287 (73.17%), Postives = 237/287 (82.58%), Query Frame = 0

Query: 3   EAASLALNAELLEWPKKDKRRFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEENYSN 62
           +AA      +LL W K DKRR LHVVYRVGD+DRTIKFYTEC GMKLLRKRD+PEE Y+N
Sbjct: 68  KAAQATTQDDLLTWVKNDKRRMLHVVYRVGDMDRTIKFYTECLGMKLLRKRDIPEEKYTN 127

Query: 63  AFLGFGPEDSNFVVELTYNYGVSSYDIGTGFGHFAIATQDVYKLVEDIRAKGGSITREPG 122
           AFLG+GPEDS+FV+ELTYNYGV  YDIG GFGHF IA  DV K VE ++AKGG ++REPG
Sbjct: 128 AFLGYGPEDSHFVIELTYNYGVDKYDIGAGFGHFGIAVDDVAKTVELVKAKGGKVSREPG 187

Query: 123 PVKGGSSVIAFAKDPDGYIFELIQRGPTPEPLCQVMLRVGDLERSIKFYEKALGMRLLKK 182
           PVKGG +VIAF +DPDGY FEL++RGPTPEPLCQVMLRVGDL+R+IKFYEKA GM LL+ 
Sbjct: 188 PVKGGKTVIAFIEDPDGYKFELLERGPTPEPLCQVMLRVGDLDRAIKFYEKAFGMELLRT 247

Query: 183 VDRPEYKYTLAMMGYADEVETTVLELTYNYGVTEYTKGNAYAQVAISTDDVYKSAEVVNL 242
            D PEYKYT+AMMGY  E +  VLELTYNYGVTEY KGNAYAQ+AI TDDVYK+AE + L
Sbjct: 248 RDNPEYKYTIAMMGYGPEDKFPVLELTYNYGVTEYDKGNAYAQIAIGTDDVYKTAEAIKL 307

Query: 243 VNQELGGKITRQPGPLPGIGTKIVSFLDPDGWKTVLVDNADFQKELE 290
                GGKITR+PGPLPGI TKI + LDPDGWK+V VDN DF KELE
Sbjct: 308 ----FGGKITREPGPLPGISTKITACLDPDGWKSVFVDNIDFLKELE 350

BLAST of HG10016194 vs. ExPASy Swiss-Prot
Match: P46235 (Probable lactoylglutathione lyase OS=Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633) OX=223926 GN=gloA PE=3 SV=2)

HSP 1 Score: 158.3 bits (399), Expect = 1.3e-37
Identity = 71/124 (57.26%), Postives = 91/124 (73.39%), Query Frame = 0

Query: 23  RFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEENYSNAFLGFGPEDSNFVVELTYNY 82
           R LH + RVGDLD++IKFYTE  GM+LLR  +  E  Y+ AF+G+G E    V+ELTYN+
Sbjct: 5   RILHTMLRVGDLDKSIKFYTEVMGMQLLRTNENKEYEYTLAFVGYGDESQGAVIELTYNW 64

Query: 83  GVSSYDIGTGFGHFAIATQDVYKLVEDIRAKGGSITREPGPVKGGSSVIAFAKDPDGYIF 142
           G + YD+GT FGH AI   D+Y   + I+A GG++TRE GPVKGG++ IAF KDPDGY+ 
Sbjct: 65  GKTEYDLGTAFGHIAIGVDDIYATCDAIKAAGGNVTREAGPVKGGTTHIAFVKDPDGYMI 124

Query: 143 ELIQ 147
           ELIQ
Sbjct: 125 ELIQ 128

BLAST of HG10016194 vs. ExPASy TrEMBL
Match: A0A6J1GDS2 (Lactoylglutathione lyase OS=Cucurbita moschata OX=3662 GN=LOC111453257 PE=3 SV=1)

HSP 1 Score: 575.5 bits (1482), Expect = 1.3e-160
Identity = 280/291 (96.22%), Postives = 286/291 (98.28%), Query Frame = 0

Query: 1   MAEAASLALNAELLEWPKKDKRRFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEENY 60
           MAEAASLA NA+LLEWPKKD+RRFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEE Y
Sbjct: 1   MAEAASLAPNADLLEWPKKDRRRFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKY 60

Query: 61  SNAFLGFGPEDSNFVVELTYNYGVSSYDIGTGFGHFAIATQDVYKLVEDIRAKGGSITRE 120
           SNAFLGFGPEDSNFVVELTYNYG+SSYDIGTGFGHFAIA+QDVYKLVEDIRAKGGSITRE
Sbjct: 61  SNAFLGFGPEDSNFVVELTYNYGISSYDIGTGFGHFAIASQDVYKLVEDIRAKGGSITRE 120

Query: 121 PGPVKGGSSVIAFAKDPDGYIFELIQRGPTPEPLCQVMLRVGDLERSIKFYEKALGMRLL 180
           PGPVKGGSSVIAFAKDPDGYIFELIQRGPTPEPLCQVMLRVGDLERSIKFYEKALGMRLL
Sbjct: 121 PGPVKGGSSVIAFAKDPDGYIFELIQRGPTPEPLCQVMLRVGDLERSIKFYEKALGMRLL 180

Query: 181 KKVDRPEYKYTLAMMGYADEVETTVLELTYNYGVTEYTKGNAYAQVAISTDDVYKSAEVV 240
           KKVDRPEYKYTLAMMGYADE+ETTVLELTYNYGVTEYTKGNAYAQVAI TDDVYKSAEV+
Sbjct: 181 KKVDRPEYKYTLAMMGYADELETTVLELTYNYGVTEYTKGNAYAQVAIGTDDVYKSAEVI 240

Query: 241 NLVNQELGGKITRQPGPLPGIGTKIVSFLDPDGWKTVLVDNADFQKELEHQ 292
            LVNQELGGKITRQ GPLPGIGTKIVSFLDPDGWKTVLVDNADFQKELEHQ
Sbjct: 241 KLVNQELGGKITRQAGPLPGIGTKIVSFLDPDGWKTVLVDNADFQKELEHQ 291

BLAST of HG10016194 vs. ExPASy TrEMBL
Match: A0A6J1IP78 (Glyoxalase I OS=Cucurbita maxima OX=3661 GN=LOC111478693 PE=3 SV=1)

HSP 1 Score: 573.2 bits (1476), Expect = 6.3e-160
Identity = 280/291 (96.22%), Postives = 285/291 (97.94%), Query Frame = 0

Query: 1   MAEAASLALNAELLEWPKKDKRRFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEENY 60
           MAEAA LA NA+LLEWPKKDKRRFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEE Y
Sbjct: 1   MAEAAFLAPNADLLEWPKKDKRRFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKY 60

Query: 61  SNAFLGFGPEDSNFVVELTYNYGVSSYDIGTGFGHFAIATQDVYKLVEDIRAKGGSITRE 120
           SNAFLGFGPEDSNFVVELTYNYGVSSYDIG+GFGHFAIA+QDVYKLVEDIRAKGGSITRE
Sbjct: 61  SNAFLGFGPEDSNFVVELTYNYGVSSYDIGSGFGHFAIASQDVYKLVEDIRAKGGSITRE 120

Query: 121 PGPVKGGSSVIAFAKDPDGYIFELIQRGPTPEPLCQVMLRVGDLERSIKFYEKALGMRLL 180
           PGPVKGGSSVIAFAKDPDGYIFELIQRGPTPEPLCQVMLRVGDLERSIKFYEKALGMRLL
Sbjct: 121 PGPVKGGSSVIAFAKDPDGYIFELIQRGPTPEPLCQVMLRVGDLERSIKFYEKALGMRLL 180

Query: 181 KKVDRPEYKYTLAMMGYADEVETTVLELTYNYGVTEYTKGNAYAQVAISTDDVYKSAEVV 240
           KKVDRPEYKYTLAMMGYADE+ETTVLELTYNYGVTEYTKGNAYAQVAI TDDVYKSAEV+
Sbjct: 181 KKVDRPEYKYTLAMMGYADELETTVLELTYNYGVTEYTKGNAYAQVAIGTDDVYKSAEVI 240

Query: 241 NLVNQELGGKITRQPGPLPGIGTKIVSFLDPDGWKTVLVDNADFQKELEHQ 292
            LVNQELGGKITRQ GPLPGIGTKIVSFLDPDGWKTVLVDNADFQKELEHQ
Sbjct: 241 KLVNQELGGKITRQAGPLPGIGTKIVSFLDPDGWKTVLVDNADFQKELEHQ 291

BLAST of HG10016194 vs. ExPASy TrEMBL
Match: A0A1S3B032 (Lactoylglutathione lyase OS=Cucumis melo OX=3656 GN=LOC103484618 PE=3 SV=1)

HSP 1 Score: 570.9 bits (1470), Expect = 3.1e-159
Identity = 282/292 (96.58%), Postives = 284/292 (97.26%), Query Frame = 0

Query: 1   MAE-AASLALNAELLEWPKKDKRRFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEN 60
           MAE AASLA NAELL+WPKKDKRRFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEE 
Sbjct: 1   MAEAAASLAPNAELLDWPKKDKRRFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEK 60

Query: 61  YSNAFLGFGPEDSNFVVELTYNYGVSSYDIGTGFGHFAIATQDVYKLVEDIRAKGGSITR 120
           YSNAFLGFGPEDSNFVVELTYNYGVSSYDIGTGFGHFAIATQDVYKLVEDIRAKGG ITR
Sbjct: 61  YSNAFLGFGPEDSNFVVELTYNYGVSSYDIGTGFGHFAIATQDVYKLVEDIRAKGGIITR 120

Query: 121 EPGPVKGGSSVIAFAKDPDGYIFELIQRGPTPEPLCQVMLRVGDLERSIKFYEKALGMRL 180
           EPGPVKGGSSVIAFAKDPDGYIFELIQRGPTPEPLCQVMLRVGDLERSIKFYEKALGMRL
Sbjct: 121 EPGPVKGGSSVIAFAKDPDGYIFELIQRGPTPEPLCQVMLRVGDLERSIKFYEKALGMRL 180

Query: 181 LKKVDRPEYKYTLAMMGYADEVETTVLELTYNYGVTEYTKGNAYAQVAISTDDVYKSAEV 240
           LKKVDRPEYKYTLAMMGYADE+ETTVLELTYNYGVTEYTKGNAYAQVAI TDDVYKSAEV
Sbjct: 181 LKKVDRPEYKYTLAMMGYADELETTVLELTYNYGVTEYTKGNAYAQVAIGTDDVYKSAEV 240

Query: 241 VNLVNQELGGKITRQPGPLPGIGTKIVSFLDPDGWKTVLVDNADFQKELEHQ 292
           VN VNQELGGKITRQPGPLPGIGTKIV FLDPDGWKTVLVDNADF KELEHQ
Sbjct: 241 VNQVNQELGGKITRQPGPLPGIGTKIVGFLDPDGWKTVLVDNADFLKELEHQ 292

BLAST of HG10016194 vs. ExPASy TrEMBL
Match: A0A5D3CNL5 (Lactoylglutathione lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G006420 PE=3 SV=1)

HSP 1 Score: 570.9 bits (1470), Expect = 3.1e-159
Identity = 282/292 (96.58%), Postives = 284/292 (97.26%), Query Frame = 0

Query: 1   MAE-AASLALNAELLEWPKKDKRRFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEN 60
           MAE AASLA NAELL+WPKKDKRRFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEE 
Sbjct: 1   MAEAAASLAPNAELLDWPKKDKRRFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEK 60

Query: 61  YSNAFLGFGPEDSNFVVELTYNYGVSSYDIGTGFGHFAIATQDVYKLVEDIRAKGGSITR 120
           YSNAFLGFGPEDSNFVVELTYNYGVSSYDIGTGFGHFAIATQDVYKLVEDIRAKGG ITR
Sbjct: 61  YSNAFLGFGPEDSNFVVELTYNYGVSSYDIGTGFGHFAIATQDVYKLVEDIRAKGGIITR 120

Query: 121 EPGPVKGGSSVIAFAKDPDGYIFELIQRGPTPEPLCQVMLRVGDLERSIKFYEKALGMRL 180
           EPGPVKGGSSVIAFAKDPDGYIFELIQRGPTPEPLCQVMLRVGDLERSIKFYEKALGMRL
Sbjct: 121 EPGPVKGGSSVIAFAKDPDGYIFELIQRGPTPEPLCQVMLRVGDLERSIKFYEKALGMRL 180

Query: 181 LKKVDRPEYKYTLAMMGYADEVETTVLELTYNYGVTEYTKGNAYAQVAISTDDVYKSAEV 240
           LKKVDRPEYKYTLAMMGYADE+ETTVLELTYNYGVTEYTKGNAYAQVAI TDDVYKSAEV
Sbjct: 181 LKKVDRPEYKYTLAMMGYADELETTVLELTYNYGVTEYTKGNAYAQVAIGTDDVYKSAEV 240

Query: 241 VNLVNQELGGKITRQPGPLPGIGTKIVSFLDPDGWKTVLVDNADFQKELEHQ 292
           VN VNQELGGKITRQPGPLPGIGTKIV FLDPDGWKTVLVDNADF KELEHQ
Sbjct: 241 VNQVNQELGGKITRQPGPLPGIGTKIVGFLDPDGWKTVLVDNADFLKELEHQ 292

BLAST of HG10016194 vs. ExPASy TrEMBL
Match: A0A0A0KJW2 (Glyoxalase I OS=Cucumis sativus OX=3659 GN=Csa_6G513680 PE=3 SV=1)

HSP 1 Score: 568.5 bits (1464), Expect = 1.6e-158
Identity = 281/292 (96.23%), Postives = 285/292 (97.60%), Query Frame = 0

Query: 1   MAE-AASLALNAELLEWPKKDKRRFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEN 60
           MAE AASLA NAELLEWPKKDKRRFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEE 
Sbjct: 1   MAEAAASLAPNAELLEWPKKDKRRFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEK 60

Query: 61  YSNAFLGFGPEDSNFVVELTYNYGVSSYDIGTGFGHFAIATQDVYKLVEDIRAKGGSITR 120
           YSNAFLGFGPEDSNFVVELTYNYGVSSYDIG+GFGHFAIATQDVYKLVEDIRAKGG ITR
Sbjct: 61  YSNAFLGFGPEDSNFVVELTYNYGVSSYDIGSGFGHFAIATQDVYKLVEDIRAKGGIITR 120

Query: 121 EPGPVKGGSSVIAFAKDPDGYIFELIQRGPTPEPLCQVMLRVGDLERSIKFYEKALGMRL 180
           EPGPVKGGSSVIAFAKDPDGYIFELIQRGPTPEPLCQVMLRVGDLERSIKFY+KALGMRL
Sbjct: 121 EPGPVKGGSSVIAFAKDPDGYIFELIQRGPTPEPLCQVMLRVGDLERSIKFYDKALGMRL 180

Query: 181 LKKVDRPEYKYTLAMMGYADEVETTVLELTYNYGVTEYTKGNAYAQVAISTDDVYKSAEV 240
           LKKVDRPEYKYTLAMMGYADE ETTVLELTYNYGVTEYTKGNAYAQVAI TDDVYKSAEV
Sbjct: 181 LKKVDRPEYKYTLAMMGYADEPETTVLELTYNYGVTEYTKGNAYAQVAIGTDDVYKSAEV 240

Query: 241 VNLVNQELGGKITRQPGPLPGIGTKIVSFLDPDGWKTVLVDNADFQKELEHQ 292
           VN VNQELGGKITRQPGPLPGIGTKIVSFLDPDGWKTVLVDNADFQKEL++Q
Sbjct: 241 VNQVNQELGGKITRQPGPLPGIGTKIVSFLDPDGWKTVLVDNADFQKELDNQ 292

BLAST of HG10016194 vs. TAIR 10
Match: AT1G11840.6 (glyoxalase I homolog )

HSP 1 Score: 508.1 bits (1307), Expect = 4.8e-144
Identity = 239/284 (84.15%), Postives = 263/284 (92.61%), Query Frame = 0

Query: 6   SLALNAELLEWPKKDKRRFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEENYSNAFL 65
           ++A  ++LLEWPKKD RRFLHVVYRVGDLDRTI+FYTE FGMKLLRKRD+PEE YSNAFL
Sbjct: 39  NMAEASDLLEWPKKDNRRFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPEEKYSNAFL 98

Query: 66  GFGPEDSNFVVELTYNYGVSSYDIGTGFGHFAIATQDVYKLVEDIRAKGGSITREPGPVK 125
           GFGPE SNFVVELTYNYGVSSYDIGTGFGHFAI+TQDV KLVE++RAKGG++TREPGPVK
Sbjct: 99  GFGPETSNFVVELTYNYGVSSYDIGTGFGHFAISTQDVSKLVENVRAKGGNVTREPGPVK 158

Query: 126 GGSSVIAFAKDPDGYIFELIQRGPTPEPLCQVMLRVGDLERSIKFYEKALGMRLLKKVDR 185
           GG SVIAF KDPDGY FELIQRGPTPEP CQVMLRVGDL+R+IKFYEKALGMRLL+K++R
Sbjct: 159 GGGSVIAFVKDPDGYTFELIQRGPTPEPFCQVMLRVGDLDRAIKFYEKALGMRLLRKIER 218

Query: 186 PEYKYTLAMMGYADEVETTVLELTYNYGVTEYTKGNAYAQVAISTDDVYKSAEVVNLVNQ 245
           PEYKYT+ MMGYA+E E+ VLELTYNY VTEYTKGNAYAQ+AI TDDVYKS EV+ +VNQ
Sbjct: 219 PEYKYTIGMMGYAEEYESIVLELTYNYDVTEYTKGNAYAQIAIGTDDVYKSGEVIKIVNQ 278

Query: 246 ELGGKITRQPGPLPGIGTKIVSFLDPDGWKTVLVDNADFQKELE 290
           ELGGKITR+ GPLPG+GTKIVSFLDPDGWKTVLVDN DF KELE
Sbjct: 279 ELGGKITREAGPLPGLGTKIVSFLDPDGWKTVLVDNKDFLKELE 322

BLAST of HG10016194 vs. TAIR 10
Match: AT1G11840.2 (glyoxalase I homolog )

HSP 1 Score: 507.7 bits (1306), Expect = 6.3e-144
Identity = 239/283 (84.45%), Postives = 262/283 (92.58%), Query Frame = 0

Query: 7   LALNAELLEWPKKDKRRFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEENYSNAFLG 66
           +A  ++LLEWPKKD RRFLHVVYRVGDLDRTI+FYTE FGMKLLRKRD+PEE YSNAFLG
Sbjct: 1   MAEASDLLEWPKKDNRRFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPEEKYSNAFLG 60

Query: 67  FGPEDSNFVVELTYNYGVSSYDIGTGFGHFAIATQDVYKLVEDIRAKGGSITREPGPVKG 126
           FGPE SNFVVELTYNYGVSSYDIGTGFGHFAI+TQDV KLVE++RAKGG++TREPGPVKG
Sbjct: 61  FGPETSNFVVELTYNYGVSSYDIGTGFGHFAISTQDVSKLVENVRAKGGNVTREPGPVKG 120

Query: 127 GSSVIAFAKDPDGYIFELIQRGPTPEPLCQVMLRVGDLERSIKFYEKALGMRLLKKVDRP 186
           G SVIAF KDPDGY FELIQRGPTPEP CQVMLRVGDL+R+IKFYEKALGMRLL+K++RP
Sbjct: 121 GGSVIAFVKDPDGYTFELIQRGPTPEPFCQVMLRVGDLDRAIKFYEKALGMRLLRKIERP 180

Query: 187 EYKYTLAMMGYADEVETTVLELTYNYGVTEYTKGNAYAQVAISTDDVYKSAEVVNLVNQE 246
           EYKYT+ MMGYA+E E+ VLELTYNY VTEYTKGNAYAQ+AI TDDVYKS EV+ +VNQE
Sbjct: 181 EYKYTIGMMGYAEEYESIVLELTYNYDVTEYTKGNAYAQIAIGTDDVYKSGEVIKIVNQE 240

Query: 247 LGGKITRQPGPLPGIGTKIVSFLDPDGWKTVLVDNADFQKELE 290
           LGGKITR+ GPLPG+GTKIVSFLDPDGWKTVLVDN DF KELE
Sbjct: 241 LGGKITREAGPLPGLGTKIVSFLDPDGWKTVLVDNKDFLKELE 283

BLAST of HG10016194 vs. TAIR 10
Match: AT1G11840.3 (glyoxalase I homolog )

HSP 1 Score: 507.7 bits (1306), Expect = 6.3e-144
Identity = 239/283 (84.45%), Postives = 262/283 (92.58%), Query Frame = 0

Query: 7   LALNAELLEWPKKDKRRFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEENYSNAFLG 66
           +A  ++LLEWPKKD RRFLHVVYRVGDLDRTI+FYTE FGMKLLRKRD+PEE YSNAFLG
Sbjct: 1   MAEASDLLEWPKKDNRRFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPEEKYSNAFLG 60

Query: 67  FGPEDSNFVVELTYNYGVSSYDIGTGFGHFAIATQDVYKLVEDIRAKGGSITREPGPVKG 126
           FGPE SNFVVELTYNYGVSSYDIGTGFGHFAI+TQDV KLVE++RAKGG++TREPGPVKG
Sbjct: 61  FGPETSNFVVELTYNYGVSSYDIGTGFGHFAISTQDVSKLVENVRAKGGNVTREPGPVKG 120

Query: 127 GSSVIAFAKDPDGYIFELIQRGPTPEPLCQVMLRVGDLERSIKFYEKALGMRLLKKVDRP 186
           G SVIAF KDPDGY FELIQRGPTPEP CQVMLRVGDL+R+IKFYEKALGMRLL+K++RP
Sbjct: 121 GGSVIAFVKDPDGYTFELIQRGPTPEPFCQVMLRVGDLDRAIKFYEKALGMRLLRKIERP 180

Query: 187 EYKYTLAMMGYADEVETTVLELTYNYGVTEYTKGNAYAQVAISTDDVYKSAEVVNLVNQE 246
           EYKYT+ MMGYA+E E+ VLELTYNY VTEYTKGNAYAQ+AI TDDVYKS EV+ +VNQE
Sbjct: 181 EYKYTIGMMGYAEEYESIVLELTYNYDVTEYTKGNAYAQIAIGTDDVYKSGEVIKIVNQE 240

Query: 247 LGGKITRQPGPLPGIGTKIVSFLDPDGWKTVLVDNADFQKELE 290
           LGGKITR+ GPLPG+GTKIVSFLDPDGWKTVLVDN DF KELE
Sbjct: 241 LGGKITREAGPLPGLGTKIVSFLDPDGWKTVLVDNKDFLKELE 283

BLAST of HG10016194 vs. TAIR 10
Match: AT1G11840.1 (glyoxalase I homolog )

HSP 1 Score: 507.7 bits (1306), Expect = 6.3e-144
Identity = 239/283 (84.45%), Postives = 262/283 (92.58%), Query Frame = 0

Query: 7   LALNAELLEWPKKDKRRFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEENYSNAFLG 66
           +A  ++LLEWPKKD RRFLHVVYRVGDLDRTI+FYTE FGMKLLRKRD+PEE YSNAFLG
Sbjct: 1   MAEASDLLEWPKKDNRRFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPEEKYSNAFLG 60

Query: 67  FGPEDSNFVVELTYNYGVSSYDIGTGFGHFAIATQDVYKLVEDIRAKGGSITREPGPVKG 126
           FGPE SNFVVELTYNYGVSSYDIGTGFGHFAI+TQDV KLVE++RAKGG++TREPGPVKG
Sbjct: 61  FGPETSNFVVELTYNYGVSSYDIGTGFGHFAISTQDVSKLVENVRAKGGNVTREPGPVKG 120

Query: 127 GSSVIAFAKDPDGYIFELIQRGPTPEPLCQVMLRVGDLERSIKFYEKALGMRLLKKVDRP 186
           G SVIAF KDPDGY FELIQRGPTPEP CQVMLRVGDL+R+IKFYEKALGMRLL+K++RP
Sbjct: 121 GGSVIAFVKDPDGYTFELIQRGPTPEPFCQVMLRVGDLDRAIKFYEKALGMRLLRKIERP 180

Query: 187 EYKYTLAMMGYADEVETTVLELTYNYGVTEYTKGNAYAQVAISTDDVYKSAEVVNLVNQE 246
           EYKYT+ MMGYA+E E+ VLELTYNY VTEYTKGNAYAQ+AI TDDVYKS EV+ +VNQE
Sbjct: 181 EYKYTIGMMGYAEEYESIVLELTYNYDVTEYTKGNAYAQIAIGTDDVYKSGEVIKIVNQE 240

Query: 247 LGGKITRQPGPLPGIGTKIVSFLDPDGWKTVLVDNADFQKELE 290
           LGGKITR+ GPLPG+GTKIVSFLDPDGWKTVLVDN DF KELE
Sbjct: 241 LGGKITREAGPLPGLGTKIVSFLDPDGWKTVLVDNKDFLKELE 283

BLAST of HG10016194 vs. TAIR 10
Match: AT1G11840.4 (glyoxalase I homolog )

HSP 1 Score: 507.7 bits (1306), Expect = 6.3e-144
Identity = 239/283 (84.45%), Postives = 262/283 (92.58%), Query Frame = 0

Query: 7   LALNAELLEWPKKDKRRFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEENYSNAFLG 66
           +A  ++LLEWPKKD RRFLHVVYRVGDLDRTI+FYTE FGMKLLRKRD+PEE YSNAFLG
Sbjct: 1   MAEASDLLEWPKKDNRRFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPEEKYSNAFLG 60

Query: 67  FGPEDSNFVVELTYNYGVSSYDIGTGFGHFAIATQDVYKLVEDIRAKGGSITREPGPVKG 126
           FGPE SNFVVELTYNYGVSSYDIGTGFGHFAI+TQDV KLVE++RAKGG++TREPGPVKG
Sbjct: 61  FGPETSNFVVELTYNYGVSSYDIGTGFGHFAISTQDVSKLVENVRAKGGNVTREPGPVKG 120

Query: 127 GSSVIAFAKDPDGYIFELIQRGPTPEPLCQVMLRVGDLERSIKFYEKALGMRLLKKVDRP 186
           G SVIAF KDPDGY FELIQRGPTPEP CQVMLRVGDL+R+IKFYEKALGMRLL+K++RP
Sbjct: 121 GGSVIAFVKDPDGYTFELIQRGPTPEPFCQVMLRVGDLDRAIKFYEKALGMRLLRKIERP 180

Query: 187 EYKYTLAMMGYADEVETTVLELTYNYGVTEYTKGNAYAQVAISTDDVYKSAEVVNLVNQE 246
           EYKYT+ MMGYA+E E+ VLELTYNY VTEYTKGNAYAQ+AI TDDVYKS EV+ +VNQE
Sbjct: 181 EYKYTIGMMGYAEEYESIVLELTYNYDVTEYTKGNAYAQIAIGTDDVYKSGEVIKIVNQE 240

Query: 247 LGGKITRQPGPLPGIGTKIVSFLDPDGWKTVLVDNADFQKELE 290
           LGGKITR+ GPLPG+GTKIVSFLDPDGWKTVLVDN DF KELE
Sbjct: 241 LGGKITREAGPLPGLGTKIVSFLDPDGWKTVLVDNKDFLKELE 283

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038882184.11.1e-16398.63lactoylglutathione lyase GLX1 [Benincasa hispida] >XP_038882185.1 lactoylglutath... [more]
KAG6603781.19.1e-16196.91Lactoylglutathione lyase GLX1, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7033953.11.2e-16096.56hypothetical protein SDJN02_03679 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023543716.12.6e-16096.56lactoylglutathione lyase GLX1 [Cucurbita pepo subsp. pepo] >XP_023543718.1 lacto... [more]
XP_022950060.12.6e-16096.22lactoylglutathione lyase GLX1 [Cucurbita moschata] >XP_022950061.1 lactoylglutat... [more]
Match NameE-valueIdentityDescription
O653988.8e-14384.45Lactoylglutathione lyase GLX1 OS=Arabidopsis thaliana OX=3702 GN=GLX1 PE=1 SV=1[more]
Q393669.8e-14284.45Putative lactoylglutathione lyase OS=Brassica oleracea var. gemmifera OX=178616 ... [more]
Q948T62.6e-13482.01Lactoylglutathione lyase OS=Oryza sativa subsp. japonica OX=39947 GN=GLYI-11 PE=... [more]
Q8W5937.0e-12473.17Probable lactoylglutathione lyase, chloroplastic OS=Arabidopsis thaliana OX=3702... [more]
P462351.3e-3757.26Probable lactoylglutathione lyase OS=Vibrio parahaemolyticus serotype O3:K6 (str... [more]
Match NameE-valueIdentityDescription
A0A6J1GDS21.3e-16096.22Lactoylglutathione lyase OS=Cucurbita moschata OX=3662 GN=LOC111453257 PE=3 SV=1[more]
A0A6J1IP786.3e-16096.22Glyoxalase I OS=Cucurbita maxima OX=3661 GN=LOC111478693 PE=3 SV=1[more]
A0A1S3B0323.1e-15996.58Lactoylglutathione lyase OS=Cucumis melo OX=3656 GN=LOC103484618 PE=3 SV=1[more]
A0A5D3CNL53.1e-15996.58Lactoylglutathione lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A0A0KJW21.6e-15896.23Glyoxalase I OS=Cucumis sativus OX=3659 GN=Csa_6G513680 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G11840.64.8e-14484.15glyoxalase I homolog [more]
AT1G11840.26.3e-14484.45glyoxalase I homolog [more]
AT1G11840.36.3e-14484.45glyoxalase I homolog [more]
AT1G11840.16.3e-14484.45glyoxalase I homolog [more]
AT1G11840.46.3e-14484.45glyoxalase I homolog [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004360Glyoxalase/fosfomycin resistance/dioxygenase domainPFAMPF00903Glyoxalasecoord: 24..144
e-value: 7.7E-23
score: 81.1
coord: 156..275
e-value: 2.7E-13
score: 50.2
IPR004361Glyoxalase ITIGRFAMTIGR00068TIGR00068coord: 10..156
e-value: 2.6E-65
score: 216.4
coord: 154..288
e-value: 2.8E-43
score: 144.9
IPR029068Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenaseGENE3D3.10.180.10coord: 150..289
e-value: 2.5E-41
score: 143.1
IPR029068Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenaseGENE3D3.10.180.10coord: 2..148
e-value: 8.6E-55
score: 186.9
IPR029068Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenaseSUPERFAMILY54593Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenasecoord: 22..149
IPR029068Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenaseSUPERFAMILY54593Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenasecoord: 154..281
NoneNo IPR availablePANTHERPTHR46036:SF8LACTOYLGLUTATHIONE LYASE-LIKE PROTEINcoord: 13..289
NoneNo IPR availablePANTHERPTHR46036LACTOYLGLUTATHIONE LYASEcoord: 13..289
NoneNo IPR availableCDDcd16358GlxI_Nicoord: 25..145
e-value: 1.00537E-73
score: 219.96
IPR018146Glyoxalase I, conserved sitePROSITEPS00935GLYOXALASE_I_2coord: 90..102
IPR018146Glyoxalase I, conserved sitePROSITEPS00934GLYOXALASE_I_1coord: 26..47
IPR018146Glyoxalase I, conserved sitePROSITEPS00934GLYOXALASE_I_1coord: 156..177
IPR037523Vicinal oxygen chelate (VOC) domainPROSITEPS51819VOCcoord: 153..281
score: 21.133867
IPR037523Vicinal oxygen chelate (VOC) domainPROSITEPS51819VOCcoord: 23..147
score: 31.42268

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10016194.1HG10016194.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019243 methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004462 lactoylglutathione lyase activity
molecular_function GO:0046872 metal ion binding