HG10016021 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10016021
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionGDSL esterase/lipase
LocationChr03: 2209290 .. 2217550 (+)
RNA-Seq ExpressionHG10016021
SyntenyHG10016021
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGACCAAAGATCTATCTTTTCGGCGATTCCATTACAGAATTTTCTTTTGCTGATGGCGGTTGGGGATCCGCACTAGCCAATCATTTCTCTCGCAGGGTATCGGCTGCTTTTCTTTTTTACTGTTTGACTTGAGCCGCTGTAAAAATCAATTCCCAACTTCCCTTTCATCATCTTTGCTCATGTCTAATTCGAATCTGAAAATTTTTGGGTGCTCTTCATTTCTTTGAATGTCATTAGGCAGATGTGGTTGTTAGAGGATACAGTGGCTACAATACCAGATGGGCGCTTAAGGTGATTGAACGAGTCTTCCCAGCATCAGAAGAACACGGCGGTAGTGGAGACGGCGCTGCCTCGCCGCTTGCCGTGACGGTGTTCTTCGGAGCTAATGACGCTCGCCTTCCAGATAGATATGGCGCCTTCCAACATGTGCCCCTTCATGAGTACAAGCAAAATCTCATTTCAATCGTCTCCTTTTTCAAGGTATCTTCTTGCTTCACACGACTGCCATGTTCTTTTGCCAAAATATTAGCTATTTTTCGTTAACCTTACTCCATTGATTTAGCCAAGGATCTGTTGAAAAGGACTATGATCACCGGTTAATCTCCAAAGGTTTGGATTTATTGTGTTGGAATTTGAACATTGGTTAGAAGTGGAGAAGATGTTAGATGCATAAGGAATGAATGATAACGTCTTTATCAGAATGAGACTTTTTAGGTAGTTTCTAAAAGTAAAACAATGTGAGCTTTAGGCCCAAAGGGTTCAGGGGATAGAAAGATGTGAAATATGTGTGTTGTCCTAATCTTTTGTCCAAGGAAAATTAGCAGCTTTTTCACTCATAGTTTTTGGGGAAGACACCCTTTTGGTTGGCACGAATAAAGAAGATCACTTTGAATTGGGTTTTGTTTTGATGGTTAGAAAACCTTTTTGTTGAATTCTTCTCTTACTGTAATGTTAAATGCGTTGCAAGAAAAGTTTTACTTTATAATGGGACTATTCCTTTATTCTAAATCTTTTTGTTAAATGAATGCTTTGCAAGTAAAGTTTGGTTGTGGTGCTGATAGGCCCCGTATTACTAAAGTGTATGTAAGGAAGCGTGCGAAGGGAGGAGGCAAGCGTGTGTGAGGTATTTTGTTAGTTATTCTGGTGTGGGGCCCATGGAATATTGTTTGTTATTTGTTAGACGGGCCTGTAGTAGGAATTATATAGAGAAGTGGGAGAGAGAGGAGAGATTATGGAAATTTTGATCTTGTAATAGTTCTCATTGAGAATAGGAGAGGCTGGGAGCTCTCAAAACTCCCTTGGTTCTGTTGATAAATAAATTGAAGGGTTAAGACCTATCAATTTTGGTATCAGAGCGGTGTTCTAGGGCATGGTGGGAAAGATGGAAGGCAGGGTATTGGCGTTGGAGGAGAAAGTGGAGTCGGGTTTCAAGGGGTCTGAGGAGAAGATTGATAAACTAGCAACTGATATGCTAGCAATTCAAGGGCAAATGGCGTTGCTATTATCGCAAATTGATGCTACTATGACAGAGAGAGAGTCGGCATCATCAGAACGCACGATAACGGATAAAGGGAAGGGGATAGTAGAACCACCTCCCGGAGAGTCAAAAACAGGAAAAGCGCAAGAAGGGGAAACACTTGCGCCTAGCAATAAAGAGGTACCCTTATTTGATATGAGATTAAGGAAATTGGAAGTACCTATATTCAAAGGTGAAGAAGAAGAAAACCCAGATGGTTGGCTACATCGAGTTGAGCGTTATTTCATTGTGAATCGCTTAACGGAGAAGGACAAACTCGACGCGACAGTCCTGTGTCTCAAGGGTGAGGCCTTGGAGTGGTATCAGTGGGAAGAGGACTGGAAACCGATTGGAAGCTGGAGTAGATTCCGGGAGCTGTTTATAGATAGGTTTCGGCCGGCGAATGAAAATGATCGGTATGCTCATTTGATGCGACTGAAACAGGAGAAATCGGTGAGGGAATATCGGCAGCGATTTGAGCAGTATTCAACGGGCCTAAAGGATTTGGGAGAGAAAGCCCTAGAGAGTAAATTTGTATGTGGGCTTAAGGAAGAGATCCAAAGTGAAATGAGGAAGCTAAGTCCAGTAGGGATTAAGGCCAAGATGACAATGGCCCAATTAATTGAGGAAGACCAAATAATCCAACAGAAAAGGCTGAAGGGAGATGGGCCGTCCAGTAGCTCGGGTGGAAAAGCAAGCCCAAGCTCGAGTGGGTCATCCGGGTCAACTGGAGCGGGTGGATCTGGTGGGTCGGGTGGGTCAAATTATGCCTGGTCGTTTACATTGAGCCCTAATCGCAAAACGGGAAATCTTGGCAGTTCGACAACGACTGTCACACCTCTTCGCGATCAGGAAAGCAAAAACACGGCGACCCAACCGTACCGGCGACTAACAGAGGAGGAGATGAGGATCAAGAAAGAAAAAGGGATTTGTTTCAAATGTGATGGGAAGTTTAGTTTTGGGCATCGATGCAAGAAAAAAGAACTTCAGTTTATGTTCGTGCAAGATGGGGAAGACATTCGCCATGGGAGGATGAGGAAATAGAGCATGACTGTATCAACAGGGATGGAGGAACAGCTCAAATCGCGGCTGAGAAAGAGGTAGCTCATCTATCTCTTGCTTCTATGGCTGGTCTGAATTCTCCTCGAACGTTGAAAGTAAAAGGCACGATCCGGGACAGAGAGGTTGTAGTGCTGATTGATGGAGGGGCCACGCATAACTTCATAGATGAGAATTTGGTTGCTTCGCTTCAATTACCTCTATCAGAAACCAATAGTTATGGGGTGGTGCTTGGAGCGGGAGGATCCATACAAGGCGCCGGAATCTGCAAAGGTGTGACTCTTACGATTTCTGATTTAACCATTAACCATGATTTCCTTCCCTTGCCCCTGGGTAGTGCAGATGTCATATTGGGTGTGGTGTGGCTGGAAACGTTGGGAAAAATTGTGATTGATTCCCAAGCATCAGTTATGGAATTCGCAGTAGGTGAGTGGTTGGTGGAGTTACGTGGAGACCAGAGCCTCGTGAAGTCTCAGATATCTCTGAAATCGCTTATGAAATCCATGGAAGGAGAGGACCAAGCTCTTTTAATTGAACTCAGCACCGTAGAATATGAGTTTGGAAAGGATAAAAGTGCAGAAAGTTTAGGGTCGGCCGAGTGTCCGGAGGAAATCCAAAATGTCCTCAAATGTTATCAGCAAGTGTTTGTCAACACAAATGCATTGCCACCGTTCAGAGAACACGACCATGCTATTGAGTTAGAGAAGGGGGCAGGTTCAGTCAATGTGCGACCGTATAGATACCTACAATTTCAAAAGGATGAGATTGAGAAGCTTGTCCAAGAAATGTTAATGGCTGGAATTATACAGCCCAGTCGAAGCTCTTTCTCTAACTCAGTTCTGTTAGTCAAGAAGAAAGATGGAAGTTGGCGATTCTGTGTGGATTATCGGGCTCTAAACAATGTTACAGTGCCAGATAAGTACCCCATTCCAATAGTTGACGAATTGTTAGATGAATTGTTTGGCGCCACGATATTTTCTAAGATCGACCTTAAGTCAGGATATCATCAAATCAGAGTTAAGTCAGCGGATGTCCACAAAACAGCTTTCAGAACACACGAAGGGCCCTATGAATTCTTGGTAATGCCCTTTGGTTTGAGGAATGCTCCTTCTACATTCCAGGCAATTATGAACGATATCCTACGGCCACACTTACGTAAGTTTGTTTTGGTTTTCTTTGATGATATATTGGTGTATAGCAAAACGATAGCCGATCATGTTGAACATTTATCAAAGGTGATGGCAATTCTGTTGGATAATCAGTTTGTAGCGAATCAGAAAAAGTGTCAATTTGGGGCATCGCGTATTGAATACTTGGGGCACATTGTTTCAGCGGAAGGGGTGTCTGCTGACCTGACCAAGATTGAAGCAATGGCAAAATGGCCAGTTCCTCGGACCCTCAAGGCGCTACGGGGGTTTTTGGGGCTGACGGGGTATTATCGGCGGTTTGTCGCCAATTATGGCACCATCGCATTACCGCTAACCCAGTTACTAAAAAAGGGTAACTTTAAGTGGAATTCAGAGGCAGAAGCGGCATTCCATCGGCTCAAATCGGCCATGATGGCAGTTCCAGTGTTGGCGTTACCCAATTTCAGAGAGACATTTGTAGTAGAAACTGATGCATCTGGAGTTGGTGTGGGCGCGGTGTTGATGCAGAATAAGCAACCGCTAGCTTTCTTTAGTCAAGCTCTTCCCTTGACTCACCGTTTTAAAGCCGTGTATGAACGTGAACTTATGGCGATTGTGCTCGCAGTTCGTAAGTGGCGGCCATATCTGTTGGGTAGACGGTTTCTTGTTCGAACGGACCAGAAAAGCCTGAAATTTCTATTGGACCAACGCGTCATTGCTGGGGAGTATCAACGCTGGATCGCTAAACTGATGGGGTATGATTTTACGATCGAATATAAGAAAGGGCTAGAAAATACGGCGGCTGACGCATTGTCACGATTGCCGACAGCAATGGAGTTTGGGGTGTTGAGTATGGTTGAAGATTTAAACACGGCAGTGTTCCAAAACCAGATTGAAGCAAATGAGGAACTAAAGACAATACGACAGTCTATTGAGGAAGGGCGGTCAACGTTTCCTGGGTATTCGCTACGGGATGAGGTGTTATATTATCAAGGCCGCCTGGTTCTCCCTGAAAATTCACCTACTATTCCACTACTGTTGACTGAATTTCATAATAGCCCAATTGGGGGGCATCAAGGAGCTCTTAAAACGTATCAACGGTTAGCTCGAGAGGTCTATTGGCTGGGCATGAAAGCTCGGGTACGAACCTTTGTTGCTGAATGTGCAGTTTGCCAACAGGCGAAGTATTTAACTCTAGCACTAGCAGGGCTGCTTCAAGCTATTCCAATTCCTGACCATGTGTGGGAGGACATCGCCATGGACTTCGTGGAGGGATTACCGAAATCCGAGGGGTATGATACAATTTTAGTTGTTGTTGATCGTTTGTCCAAGTATGCGCACTTCATCCTTCTTAAACATCCCTTTACTGCCCACTCCATCGCCACAGTGTTTATCAAGGAGATTGTGCGTTTACATGGATACCCGCGTAGTATTATTTCTGATAGGGACAAAATTTTTACAAGCTTGTTTTGGGAGGAATTATTCAAGGCGTCGGGAACTCAATTGAGGAGGAGTACGACATATCACCCTCAAACAGATGGTCAATCCGAGGTGCTTAATCGTGGCCTTGAAACATACTTACGCTGCTTTACAATGGGGAGGCCCAAGCAGTGGGCAAAATGGATACCATGGGCTGAATTCAGCTACAACACTGCCACTCATTCCGCCACTAACATTTCACCGTTTGAGGTACTATACGGCCGACCCCCACCTCCGCTATTGCAGTATGAACCGAGTATGTGCGCAGTCAGCGAGGTGGACCAACAATTGAAGGCGCGGGATGAGATGCTGTCCATATTAAAGGCAACATTACAGCGAGCTCAGCAAAGGATGATGCATTTGGCTAATAAGAAGAGAAGGGAGGTAGAGTATGAAGTTAATGATTGGGTTTACTTGAAGTTGCGTCCGTACCGCCAGACATCTTTGACCCGTCATCAACATCCGAAGTTAGCACCACGATTCATTGGCCCTTATCAGATCATTGCGCGTGTAGGGAAGGTTGCTTATAAATTGGCACTACCATCGGATTCGGGTCTGCACCCTGTTTTTCATGTGTCTTTATTGAAGAAAGTGGTGGGCCAAAACATGCCTATCTTACCCATACCACCATCTTTAGGCCCAGATTTATCAATCAGGATGCAACCAACGGAGGTCTTAGGGATGCGCTCCTCGCTTACTGACACGGGTGCCTCCGAGGTGCTAATTCGATGGGAGCAAGGCCTACCAATCGACGCAACTTGGGAACTCACTACGATAATTCAAGATCATTTTCCTGAATTTCACCTTGAGGACAAGGTGGCTCTTTGGGGGGCGGGTAATGATAGGCCCCGTATTACTAAAGTGTATGTAAGGAAGCGTGCGAAGGGAGGAGGCAAGCGTGTGTGAGGTATTTTGTTAGTTATTCTGGTGTGGGGCCCATGGAATATTGTTTGTTATTTGTTAGACGGGCCTGTATTAGGAATTATATAGAGAAGTGGGAGAGAGAGGAGAGGTTATGGAAATTTTGATCTTGTAATAGTTCTCATTGAGAATAGGAGAGGCTGGGAGCTCTCAAAACTCCCTTAGTTCTGTTGATAAATAAATTGAAGGGTTAAGACCTATCAGGTGCCTACATGTTCTACTATTCACAAAAAAAAAAAAAAAAAAAAACACAATGATTTTTTCTATTTAACTTCCCTTTCTAGCAATTCAAGTGGAGTCTGAGTTTGCTTCTATTGGATTTGCAGAAGAAATGGCCTGCAACTCGTATTCTGCTTATCACTCCTCCTCCTATTGATGAAGAAGGGCGTTTACAGTATGGTTATCTTTCCTACTGTCTTGATTTAGCTCAAACTATTTTGAGAGATCTCGAGTCTCGAGTTTTTAAATTGTACTTCTGATAATGGGAAATGATACAATCTTTGCAGGAACCCTTACGTTTCAAATCCATTAAATGAGCCTGAGAGGACTAACAATGCAGCTGGTGATTATGCCAAGGCTTGCATTGCTGTTGCTACAGAATGTGGGGTTTCTGTGATTGATATTTGGACTAAGATGCAGCAAGTTCCTGGCTGGGAAAAGGCTTGTTTAAGGTATTTATGAGTTCACTCTGATGTTTGCGCATCTTTTACTCAGCAATTGCTTCGAATAATCTGCTGGCCTCTTTACCATATTATTTGTATGTTTTGTCGAACTCTGTATAAATTTTCCTGGAATTAACAAGATGTGGAATTATTTCAGGTCATTAAAATTTGTAAACTTGGACAAGATTACATTTTTGTCACATCACGTGGCAATAGAATTAGATGTAATATAATGCTTAATCTTAAGCTGCCCTTTTTCCTTCCTCTTTGGTGGACAGTTAGGCTCTGTCAAATCACTTCTCTTTCCTAACAGTTTTACTCTGTATGCTGTTAAATATCTGGCACTAGATTGAAAAGCTTCTGGAGCTATTATTCAGATAGCTAAAACAAAACGAAAATGGGGTTACTCAGTATAAAACGTATCATTCGAGAGGACTAATCTAATACTCCCCTCACAAGTCACATCTAACGCCTGTGTGAATATTCCCCTTGGCTAATAGACTACTCCTATATCTCCGCTTTGCTTTTTAACAAGCTACTTTAAATTATTTATGCACTATTTGATAACCATTTGGTTTTTGTTTCAAATTTTTAAAATTGGATTTGTTTTCTCATAATTCCTTTACGATGGTTTTATTCTTAAGGAATCGTTTGAGCTTTTAACCAAATTTCAAAAACCAAAACAAATTTTTGAAAACCACTTTTTTTTAGTTTCCAAATTTTTTTTTTTTGGATTTTGAAAACATTTGTAAAAAGTAGACAACCAAATAAAAAAAAACCCCTAGAAGTAGTGTTTATAAGCTTAATTTTTTAGGAAAAAAACATGAACCAACAACCAAATGGTAACTAAAAAGACGTTAGTTACTCTTCACATATGCGGCCAATTGCTTCTACTTCCCTGCTTTTCCCTTTTCTCTGCTTGAATAGGCATACCAAATTGGAGGCCTATATTGAGACAGACAGCAAAACTAGCCAGCTCAGCCGATTGGCGCTGCTCTGAGTTAATATCTAGAAGCCTATAACATATCTGTTGCTCATGGTTCTGCTTAAGGTGCTGTTTGGATGTCTATTCTTCGAATATTTAGAAACTGCTTTATGATCAAGTTTATCATAAATATGCATTTATATATACTATTCCTGGCTTTTGTTTTGTCTCATCCTTAGTCCCTCTCAAGTTGAAAAGACCCTTGAAAACAAACTCCAATGGAAAATAACTTTGAAAAAGTCGCTATCCAATCAGATTTTTGTGTTAAAGGCATCTTGATTTCATAGAAATGAGGACAACTTATTGCTGTGTAAGATCTGAACCTAATCTATCCATTTGTTTGCAGTGATGGGCTGCACCTGACTCGAAATGGCAACACGATTGTGTTCGAGGAAGTTGTCAAGAGGCTGAAAGAAGAAGGATTGAGTCCAGAAATGCTGCGTGCTGATCTTCCACTTTTTTCGGAAATAGACTCTAAAAATCCTCTCAAAGCTTTTGAAAAATTATGA

mRNA sequence

ATGAGACCAAAGATCTATCTTTTCGGCGATTCCATTACAGAATTTTCTTTTGCTGATGGCGGTTGGGGATCCGCACTAGCCAATCATTTCTCTCGCAGGGCAGATGTGGTTGTTAGAGGATACAGTGGCTACAATACCAGATGGGCGCTTAAGGTGATTGAACGAGTCTTCCCAGCATCAGAAGAACACGGCGGTAGTGGAGACGGCGCTGCCTCGCCGCTTGCCGTGACGGTGTTCTTCGGAGCTAATGACGCTCGCCTTCCAGATAGATATGGCGCCTTCCAACATGTGCCCCTTCATGAGTACAAGCAAAATCTCATTTCAATCGTCTCCTTTTTCAAGAAGAAATGGCCTGCAACTCGTATTCTGCTTATCACTCCTCCTCCTATTGATGAAGAAGGGCGTTTACAGAACCCTTACGTTTCAAATCCATTAAATGAGCCTGAGAGGACTAACAATGCAGCTGGTGATTATGCCAAGGCTTGCATTGCTGTTGCTACAGAATGTGGGGTTTCTGTGATTGATATTTGGACTAAGATGCAGCAAGTTCCTGGCTGGGAAAAGGCTTGTTTAAGTGATGGGCTGCACCTGACTCGAAATGGCAACACGATTGTGTTCGAGGAAGTTGTCAAGAGGCTGAAAGAAGAAGGATTGAGTCCAGAAATGCTGCGTGCTGATCTTCCACTTTTTTCGGAAATAGACTCTAAAAATCCTCTCAAAGCTTTTGAAAAATTATGA

Coding sequence (CDS)

ATGAGACCAAAGATCTATCTTTTCGGCGATTCCATTACAGAATTTTCTTTTGCTGATGGCGGTTGGGGATCCGCACTAGCCAATCATTTCTCTCGCAGGGCAGATGTGGTTGTTAGAGGATACAGTGGCTACAATACCAGATGGGCGCTTAAGGTGATTGAACGAGTCTTCCCAGCATCAGAAGAACACGGCGGTAGTGGAGACGGCGCTGCCTCGCCGCTTGCCGTGACGGTGTTCTTCGGAGCTAATGACGCTCGCCTTCCAGATAGATATGGCGCCTTCCAACATGTGCCCCTTCATGAGTACAAGCAAAATCTCATTTCAATCGTCTCCTTTTTCAAGAAGAAATGGCCTGCAACTCGTATTCTGCTTATCACTCCTCCTCCTATTGATGAAGAAGGGCGTTTACAGAACCCTTACGTTTCAAATCCATTAAATGAGCCTGAGAGGACTAACAATGCAGCTGGTGATTATGCCAAGGCTTGCATTGCTGTTGCTACAGAATGTGGGGTTTCTGTGATTGATATTTGGACTAAGATGCAGCAAGTTCCTGGCTGGGAAAAGGCTTGTTTAAGTGATGGGCTGCACCTGACTCGAAATGGCAACACGATTGTGTTCGAGGAAGTTGTCAAGAGGCTGAAAGAAGAAGGATTGAGTCCAGAAATGCTGCGTGCTGATCTTCCACTTTTTTCGGAAATAGACTCTAAAAATCCTCTCAAAGCTTTTGAAAAATTATGA

Protein sequence

MRPKIYLFGDSITEFSFADGGWGSALANHFSRRADVVVRGYSGYNTRWALKVIERVFPASEEHGGSGDGAASPLAVTVFFGANDARLPDRYGAFQHVPLHEYKQNLISIVSFFKKKWPATRILLITPPPIDEEGRLQNPYVSNPLNEPERTNNAAGDYAKACIAVATECGVSVIDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLKEEGLSPEMLRADLPLFSEIDSKNPLKAFEKL
Homology
BLAST of HG10016021 vs. NCBI nr
Match: XP_038882981.1 (GDSL esterase/lipase At5g45920 [Benincasa hispida] >XP_038882982.1 GDSL esterase/lipase At5g45920 [Benincasa hispida])

HSP 1 Score: 484.6 bits (1246), Expect = 5.1e-133
Identity = 231/245 (94.29%), Postives = 238/245 (97.14%), Query Frame = 0

Query: 1   MRPKIYLFGDSITEFSFADGGWGSALANHFSRRADVVVRGYSGYNTRWALKVIERVFPAS 60
           MRPKIYLFGDSITEFSFADGGWGSALANHFSRRADVVVRGYSGYNTRWALKVIERVFP+S
Sbjct: 1   MRPKIYLFGDSITEFSFADGGWGSALANHFSRRADVVVRGYSGYNTRWALKVIERVFPSS 60

Query: 61  EEHGGSGDGAASPLAVTVFFGANDARLPDRYGAFQHVPLHEYKQNLISIVSFFKKKWPAT 120
           EEHGGSGDGAASPLA TVFFGANDA LPDRYGAFQHVPLHEYKQNLISIVSFFKKKWPAT
Sbjct: 61  EEHGGSGDGAASPLAATVFFGANDACLPDRYGAFQHVPLHEYKQNLISIVSFFKKKWPAT 120

Query: 121 RILLITPPPIDEEGRLQNPYVSNPLNEPERTNNAAGDYAKACIAVATECGVSVIDIWTKM 180
           RIL+ITPPPIDEEGRLQNPYVSNPLNEPERTNNAAGDYAKACIAVA ECG+SVIDIWTKM
Sbjct: 121 RILIITPPPIDEEGRLQNPYVSNPLNEPERTNNAAGDYAKACIAVAAECGISVIDIWTKM 180

Query: 181 QQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLKEEGLSPEMLRADLPLFSEIDSKNPLK 240
           QQVPGWEKACLSDGLHLTRNGNTIVFEEVVK+L+EEGLSPE L A+LPLFSEIDS NPLK
Sbjct: 181 QQVPGWEKACLSDGLHLTRNGNTIVFEEVVKKLEEEGLSPEKLPAELPLFSEIDSNNPLK 240

Query: 241 AFEKL 246
           AF +L
Sbjct: 241 AFGEL 245

BLAST of HG10016021 vs. NCBI nr
Match: XP_022978753.1 (GDSL esterase/lipase At5g45920 [Cucurbita maxima])

HSP 1 Score: 456.8 bits (1174), Expect = 1.1e-124
Identity = 219/245 (89.39%), Postives = 231/245 (94.29%), Query Frame = 0

Query: 1   MRPKIYLFGDSITEFSFADGGWGSALANHFSRRADVVVRGYSGYNTRWALKVIERVFPAS 60
           MRP+IYLFGDSITEFSFADGGWG+ALANHFSRRADVVVRGYSGYNTRWALKVIE VFPAS
Sbjct: 1   MRPRIYLFGDSITEFSFADGGWGAALANHFSRRADVVVRGYSGYNTRWALKVIEHVFPAS 60

Query: 61  EEHGGSGDGAASPLAVTVFFGANDARLPDRYGAFQHVPLHEYKQNLISIVSFFKKKWPAT 120
           EEHGG+GD    PLA+TVFFGANDA LPDRYGAFQHVPL EYKQNLISIVSF KK+WPAT
Sbjct: 61  EEHGGAGD----PLALTVFFGANDACLPDRYGAFQHVPLDEYKQNLISIVSFLKKQWPAT 120

Query: 121 RILLITPPPIDEEGRLQNPYVSNPLNEPERTNNAAGDYAKACIAVATECGVSVIDIWTKM 180
           RILLITPPPIDEEGRLQNPY+ NPLNEPERTNNAAGDYAKACIAVA ECGVSVIDIWTKM
Sbjct: 121 RILLITPPPIDEEGRLQNPYIVNPLNEPERTNNAAGDYAKACIAVAAECGVSVIDIWTKM 180

Query: 181 QQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLKEEGLSPEMLRADLPLFSEIDSKNPLK 240
           QQ+PGWEKACLSDGLHL+RNGNTIVFEEVV++LKEEGLSPEML ADLPL SEID  +PLK
Sbjct: 181 QQLPGWEKACLSDGLHLSRNGNTIVFEEVVQKLKEEGLSPEMLPADLPLLSEIDYNDPLK 240

Query: 241 AFEKL 246
           AFE+L
Sbjct: 241 AFEEL 241

BLAST of HG10016021 vs. NCBI nr
Match: TYK13191.1 (GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 454.9 bits (1169), Expect = 4.4e-124
Identity = 222/245 (90.61%), Postives = 228/245 (93.06%), Query Frame = 0

Query: 1   MRPKIYLFGDSITEFSFADGGWGSALANHFSRRADVVVRGYSGYNTRWALKVIERVFPAS 60
           MRPKIYLFGDSITEFSFADGGWGSAL NHF+R+ADVVVRGYSGYNTRWALKVIERVFP S
Sbjct: 1   MRPKIYLFGDSITEFSFADGGWGSALTNHFTRKADVVVRGYSGYNTRWALKVIERVFPPS 60

Query: 61  EEHGGSGDGAASPLAVTVFFGANDARLPDRYGAFQHVPLHEYKQNLISIVSFFKKKWPAT 120
           EE       AASPLAVTVFFGANDA LPDRYGAFQHVPLHEYKQNLISIV+FFKKKWPAT
Sbjct: 61  EER-----EAASPLAVTVFFGANDACLPDRYGAFQHVPLHEYKQNLISIVTFFKKKWPAT 120

Query: 121 RILLITPPPIDEEGRLQNPYVSNPLNEPERTNNAAGDYAKACIAVATECGVSVIDIWTKM 180
           RILLITPPPIDEEGRLQNPYV NPLNEPERTN+AAG YA ACIAVA ECGVSVIDIWTKM
Sbjct: 121 RILLITPPPIDEEGRLQNPYVPNPLNEPERTNDAAGAYANACIAVAKECGVSVIDIWTKM 180

Query: 181 QQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLKEEGLSPEMLRADLPLFSEIDSKNPLK 240
           QQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRL+EEGLSPE L ADLPLFSE+DS NPLK
Sbjct: 181 QQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPETLPADLPLFSEMDSTNPLK 240

Query: 241 AFEKL 246
           AFEKL
Sbjct: 241 AFEKL 240

BLAST of HG10016021 vs. NCBI nr
Match: XP_022925816.1 (GDSL esterase/lipase At5g45920 [Cucurbita moschata] >KAG7034633.1 GDSL esterase/lipase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 454.5 bits (1168), Expect = 5.7e-124
Identity = 217/245 (88.57%), Postives = 231/245 (94.29%), Query Frame = 0

Query: 1   MRPKIYLFGDSITEFSFADGGWGSALANHFSRRADVVVRGYSGYNTRWALKVIERVFPAS 60
           MRP+IYLFGDSITEFSFADGGWG+ALANHFSRRADVVVRGYSGYNTRWALKV+ERVFPAS
Sbjct: 1   MRPRIYLFGDSITEFSFADGGWGAALANHFSRRADVVVRGYSGYNTRWALKVLERVFPAS 60

Query: 61  EEHGGSGDGAASPLAVTVFFGANDARLPDRYGAFQHVPLHEYKQNLISIVSFFKKKWPAT 120
           EEH G+GD    PLA+TVFFGANDA LPDRYGAFQHVPL EYKQNLISIVSF KK+WPAT
Sbjct: 61  EEHSGAGD----PLALTVFFGANDACLPDRYGAFQHVPLDEYKQNLISIVSFLKKQWPAT 120

Query: 121 RILLITPPPIDEEGRLQNPYVSNPLNEPERTNNAAGDYAKACIAVATECGVSVIDIWTKM 180
           RILLITPPPIDEEGRLQNPY+ NPLNEPERTNNAAGDYAKACIAVA ECGVSVIDIWTKM
Sbjct: 121 RILLITPPPIDEEGRLQNPYLENPLNEPERTNNAAGDYAKACIAVAAECGVSVIDIWTKM 180

Query: 181 QQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLKEEGLSPEMLRADLPLFSEIDSKNPLK 240
           QQ+PGWEKACLSDGLHL+RNGNTIVFEEVV++LKEEGLSPEML ADLPL SE+D  +PLK
Sbjct: 181 QQLPGWEKACLSDGLHLSRNGNTIVFEEVVQKLKEEGLSPEMLPADLPLLSEMDYNDPLK 240

Query: 241 AFEKL 246
           AFE+L
Sbjct: 241 AFEEL 241

BLAST of HG10016021 vs. NCBI nr
Match: XP_023544567.1 (GDSL esterase/lipase At5g45920 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 453.4 bits (1165), Expect = 1.3e-123
Identity = 217/245 (88.57%), Postives = 230/245 (93.88%), Query Frame = 0

Query: 1   MRPKIYLFGDSITEFSFADGGWGSALANHFSRRADVVVRGYSGYNTRWALKVIERVFPAS 60
           MRP+IYLFGDSITEFSFADGGWG ALANHFSRRADVVVRGYSGYNTRWALKV+ERVFPAS
Sbjct: 1   MRPRIYLFGDSITEFSFADGGWGGALANHFSRRADVVVRGYSGYNTRWALKVLERVFPAS 60

Query: 61  EEHGGSGDGAASPLAVTVFFGANDARLPDRYGAFQHVPLHEYKQNLISIVSFFKKKWPAT 120
           EEH G+GD    PLA+TVFFGANDA LPDRYGAFQHVPL EYKQNLISIVSF KK+WPAT
Sbjct: 61  EEHSGAGD----PLALTVFFGANDACLPDRYGAFQHVPLDEYKQNLISIVSFLKKQWPAT 120

Query: 121 RILLITPPPIDEEGRLQNPYVSNPLNEPERTNNAAGDYAKACIAVATECGVSVIDIWTKM 180
           RILLITPPPIDEEGRLQNPY+ NPLNEPERTNNAAGDYAKACIAVA ECGVSVIDIWTKM
Sbjct: 121 RILLITPPPIDEEGRLQNPYLVNPLNEPERTNNAAGDYAKACIAVAAECGVSVIDIWTKM 180

Query: 181 QQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLKEEGLSPEMLRADLPLFSEIDSKNPLK 240
           QQ+PGWEKACLSDGLHL+RNGNTIVFEEVV++LKEEGLSPEML ADLPL SE+D  +PLK
Sbjct: 181 QQLPGWEKACLSDGLHLSRNGNTIVFEEVVQKLKEEGLSPEMLPADLPLLSEMDYNDPLK 240

Query: 241 AFEKL 246
           AFE+L
Sbjct: 241 AFEEL 241

BLAST of HG10016021 vs. ExPASy Swiss-Prot
Match: Q6NMR9 (GDSL esterase/lipase At5g45920 OS=Arabidopsis thaliana OX=3702 GN=At5g45920 PE=2 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 1.7e-94
Identity = 163/244 (66.80%), Postives = 193/244 (79.10%), Query Frame = 0

Query: 1   MRPKIYLFGDSITEFSFADGGWGSALANHFSRRADVVVRGYSGYNTRWALKVIERVFPAS 60
           MR KI+LFGDSITE SF+DGGWG++LA+   R+AD+V+RGYSGYNTRWALKV+ERVFP +
Sbjct: 2   MRRKIFLFGDSITEESFSDGGWGASLADLLRRKADMVLRGYSGYNTRWALKVVERVFPVA 61

Query: 61  EEHGGSGDGAASPLAVTVFFGANDARLPDRYGAFQHVPLHEYKQNLISIVSFFKKKWPAT 120
           EE GG      SP AVTVFFGANDA LP+R   FQHVPLHEYKQNL SIVSF K +WP T
Sbjct: 62  EEDGGD-----SPAAVTVFFGANDACLPERCSGFQHVPLHEYKQNLRSIVSFLKNRWPQT 121

Query: 121 RILLITPPPIDEEGRLQNPYVSNPLNEPERTNNAAGDYAKACIAVATECGVSVIDIWTKM 180
            I+LITPPPIDEE RL+ PY+ N    PERTN  AG YAKACIAVA EC +SV D+W+KM
Sbjct: 122 AIILITPPPIDEEARLRYPYIENTTGLPERTNEVAGLYAKACIAVAEECQISVTDLWSKM 181

Query: 181 QQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLKEEGLSPEMLRADLPLFSEIDSKNPLK 240
           QQ+P W+  CL DGLHL+R GN +VFEEV K+LKEEG+  E L  DLPL  ++D K+PLK
Sbjct: 182 QQIPNWQTECLWDGLHLSRVGNKVVFEEVAKKLKEEGIGAEDLAVDLPLIEDVDPKDPLK 240

Query: 241 AFEK 245
           +F++
Sbjct: 242 SFDE 240

BLAST of HG10016021 vs. ExPASy Swiss-Prot
Match: Q9FM04 (GDSL esterase/lipase At5g62930 OS=Arabidopsis thaliana OX=3702 GN=At5g62930 PE=2 SV=3)

HSP 1 Score: 257.3 bits (656), Expect = 1.8e-67
Identity = 129/247 (52.23%), Postives = 162/247 (65.59%), Query Frame = 0

Query: 1   MRPKIYLFGDSITEFSFADGGWGSALANHFSRRADVVVRGYSGYNTRWALKVIERVFPAS 60
           MRP+I LFGDSIT  SF  GGWGSALA+ +SR+ADVVVRGY GYNTRWAL ++  +FP  
Sbjct: 1   MRPEIVLFGDSITAQSFRSGGWGSALADAYSRKADVVVRGYGGYNTRWALFLLHHIFPLG 60

Query: 61  EEHGGSGDGAASPLAVTVFFGANDARLPDRYGAFQHVPLHEYKQNLISIVSFFKKKWPAT 120
                    ++ P+A T+FFGANDA L  R    QHVP+ EY  N+  IV   KK  P  
Sbjct: 61  --------SSSPPVATTIFFGANDAALKGRTSDRQHVPVEEYTDNVRKIVQHLKKCSPTM 120

Query: 121 RILLITPPPIDEEGR---LQNPYVSNPLNEPERTNNAAGDYAKACIAVATECGVSVIDIW 180
            I+LITPPPIDE GR    ++ Y    + EPERTN   G YA+ C+A+A E G+  +++W
Sbjct: 121 LIVLITPPPIDEAGRQSYAESIYGEKAMKEPERTNETTGVYAQHCVALAEELGLRCVNLW 180

Query: 181 TKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLKEEGLSPEMLRADLPLFSEIDSKN 240
           +KMQ+   W+K  LSDGLHLT  GN +VF+EV +  +E  LSPE +  D P  S ID KN
Sbjct: 181 SKMQETNDWQKKYLSDGLHLTPEGNGVVFDEVSRVFREAWLSPEEMPFDFPHHSHIDGKN 239

Query: 241 PLKAFEK 245
           P KAFE+
Sbjct: 241 PSKAFEE 239

BLAST of HG10016021 vs. ExPASy Swiss-Prot
Match: Q9DB29 (Isoamyl acetate-hydrolyzing esterase 1 homolog OS=Mus musculus OX=10090 GN=Iah1 PE=1 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 3.6e-44
Identity = 100/238 (42.02%), Postives = 139/238 (58.40%), Query Frame = 0

Query: 3   PKIYLFGDSITEFSFADGGWGSALANHFSRRADVVVRGYSGYNTRWALKVIERVFPASEE 62
           P++ LFGDSIT+FSF  GGWGS LA+   R+ DV+ RG+SGYNTRWA  ++ R+      
Sbjct: 16  PRVLLFGDSITQFSFQQGGWGSLLADRLVRKCDVLNRGFSGYNTRWAKIILPRLI----- 75

Query: 63  HGGSGDGAASPLAVTVFFGANDARLPDRYGAFQHVPLHEYKQNLISIVSFFKK-KWPATR 122
               G G  +P+AVT+FFGAND+ L D     QHVPL EY  NL  +V + +    P  R
Sbjct: 76  --RKGPGMENPVAVTIFFGANDSSLKDE-NPKQHVPLDEYSANLRDMVQYLRSVDVPRER 135

Query: 123 ILLITPPPIDEEGRLQNPYVSN-PLNEPERTNNAAGDYAKACIAVATECGVSVIDIWTKM 182
           ++LITPPP+ E    +   +    LN   R N+  G+YA AC+ VA +CG  V+D+WT M
Sbjct: 136 VILITPPPLCEAAWEKECVLKGCKLN---RLNSVVGEYANACLQVARDCGTDVLDLWTLM 195

Query: 183 QQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLKEEGLSPEMLRADLPLFSEIDSKNP 239
           Q+      + LSDGLHL+  GN  +F  +   L ++  S   L   LP + +++   P
Sbjct: 196 QKDSQDFSSYLSDGLHLSPMGNEFLFLNLCPLLDKKVSSLPWL---LPYWKDVEEAKP 239

BLAST of HG10016021 vs. ExPASy Swiss-Prot
Match: Q711G3 (Isoamyl acetate-hydrolyzing esterase 1 homolog OS=Rattus norvegicus OX=10116 GN=Iah1 PE=2 SV=2)

HSP 1 Score: 178.3 bits (451), Expect = 1.0e-43
Identity = 101/238 (42.44%), Postives = 140/238 (58.82%), Query Frame = 0

Query: 3   PKIYLFGDSITEFSFADGGWGSALANHFSRRADVVVRGYSGYNTRWALKVIERVFPASEE 62
           P++ LFGDSIT+FSF  GGWG+ LA+   R+ DV+ RG+SGYNTRWA  ++ R+      
Sbjct: 16  PRVLLFGDSITQFSFQQGGWGTLLADRLVRKCDVLNRGFSGYNTRWAKIILPRII----- 75

Query: 63  HGGSGDGAASPLAVTVFFGANDARLPDRYGAFQHVPLHEYKQNLISIVSFFKK-KWPATR 122
               G G  +P+AVT+FFGAND+ L D     QHVPL EY  NL  +V + +    P  R
Sbjct: 76  --RKGAGLENPVAVTIFFGANDSTLKDE-NPKQHVPLDEYSANLRDMVQYLRSVDIPKER 135

Query: 123 ILLITPPPIDEEGRLQNPYVSN-PLNEPERTNNAAGDYAKACIAVATECGVSVIDIWTKM 182
           ++LITPPP+ E    +   +    LN   R N A G+YAKAC+ VA +CG  V+D+WT M
Sbjct: 136 VILITPPPLCEAAWEKECILKGCKLN---RLNVAVGEYAKACLQVARDCGTDVLDLWTLM 195

Query: 183 QQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLKEEGLSPEMLRADLPLFSEIDSKNP 239
           Q+      + LSDGLHL+  GN  +F  +   L ++  S   L   LP + +++   P
Sbjct: 196 QKDNQDFSSYLSDGLHLSPLGNEFLFFHLWPLLDKKVSSLPRL---LPDWKDVEETKP 239

BLAST of HG10016021 vs. ExPASy Swiss-Prot
Match: Q3SZ16 (Isoamyl acetate-hydrolyzing esterase 1 homolog OS=Bos taurus OX=9913 GN=IAH1 PE=2 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 1.8e-43
Identity = 100/238 (42.02%), Postives = 140/238 (58.82%), Query Frame = 0

Query: 3   PKIYLFGDSITEFSFADGGWGSALANHFSRRADVVVRGYSGYNTRWALKVIERVFPASEE 62
           P++ LFGDSIT+FSF  GGWG++LA+   R+ DV+ RG+SGYNTRWA  ++ R+      
Sbjct: 16  PRVLLFGDSITQFSFQQGGWGASLADMLVRKCDVLNRGFSGYNTRWAKIILPRLV----- 75

Query: 63  HGGSGDGAASPLAVTVFFGANDARLPDRYGAFQHVPLHEYKQNLISIVSFFKK-KWPATR 122
               G G  SP+AVT+FFGAND+ L D     QHVPL E+  NL S+V + +    P  R
Sbjct: 76  --RKGSGLDSPVAVTIFFGANDSALKDE-NPKQHVPLEEFVANLRSMVRYLRSVDVPEGR 135

Query: 123 ILLITPPPIDEEGRLQNPYVSN-PLNEPERTNNAAGDYAKACIAVATECGVSVIDIWTKM 182
           ++LITPPP+ E    Q        LN   R N+  G+YA+AC+ VA +CG   +D+W+ M
Sbjct: 136 LILITPPPLCEAAWAQECLQQGCKLN---RLNSVVGEYARACLQVAQDCGADALDLWSLM 195

Query: 183 QQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLKEEGLSPEMLRADLPLFSEIDSKNP 239
           Q+      + LSDGLHL+  GN  VF  +   ++++  S   L   LP + +I    P
Sbjct: 196 QKDGQDFSSYLSDGLHLSPKGNEFVFSHLWPLIEKKVSSLPFL---LPYWRDIAEARP 239

BLAST of HG10016021 vs. ExPASy TrEMBL
Match: A0A6J1IU69 (GDSL esterase/lipase At5g45920 OS=Cucurbita maxima OX=3661 GN=LOC111478618 PE=3 SV=1)

HSP 1 Score: 456.8 bits (1174), Expect = 5.6e-125
Identity = 219/245 (89.39%), Postives = 231/245 (94.29%), Query Frame = 0

Query: 1   MRPKIYLFGDSITEFSFADGGWGSALANHFSRRADVVVRGYSGYNTRWALKVIERVFPAS 60
           MRP+IYLFGDSITEFSFADGGWG+ALANHFSRRADVVVRGYSGYNTRWALKVIE VFPAS
Sbjct: 1   MRPRIYLFGDSITEFSFADGGWGAALANHFSRRADVVVRGYSGYNTRWALKVIEHVFPAS 60

Query: 61  EEHGGSGDGAASPLAVTVFFGANDARLPDRYGAFQHVPLHEYKQNLISIVSFFKKKWPAT 120
           EEHGG+GD    PLA+TVFFGANDA LPDRYGAFQHVPL EYKQNLISIVSF KK+WPAT
Sbjct: 61  EEHGGAGD----PLALTVFFGANDACLPDRYGAFQHVPLDEYKQNLISIVSFLKKQWPAT 120

Query: 121 RILLITPPPIDEEGRLQNPYVSNPLNEPERTNNAAGDYAKACIAVATECGVSVIDIWTKM 180
           RILLITPPPIDEEGRLQNPY+ NPLNEPERTNNAAGDYAKACIAVA ECGVSVIDIWTKM
Sbjct: 121 RILLITPPPIDEEGRLQNPYIVNPLNEPERTNNAAGDYAKACIAVAAECGVSVIDIWTKM 180

Query: 181 QQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLKEEGLSPEMLRADLPLFSEIDSKNPLK 240
           QQ+PGWEKACLSDGLHL+RNGNTIVFEEVV++LKEEGLSPEML ADLPL SEID  +PLK
Sbjct: 181 QQLPGWEKACLSDGLHLSRNGNTIVFEEVVQKLKEEGLSPEMLPADLPLLSEIDYNDPLK 240

Query: 241 AFEKL 246
           AFE+L
Sbjct: 241 AFEEL 241

BLAST of HG10016021 vs. ExPASy TrEMBL
Match: A0A5D3CMZ1 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G008030 PE=4 SV=1)

HSP 1 Score: 454.9 bits (1169), Expect = 2.1e-124
Identity = 222/245 (90.61%), Postives = 228/245 (93.06%), Query Frame = 0

Query: 1   MRPKIYLFGDSITEFSFADGGWGSALANHFSRRADVVVRGYSGYNTRWALKVIERVFPAS 60
           MRPKIYLFGDSITEFSFADGGWGSAL NHF+R+ADVVVRGYSGYNTRWALKVIERVFP S
Sbjct: 1   MRPKIYLFGDSITEFSFADGGWGSALTNHFTRKADVVVRGYSGYNTRWALKVIERVFPPS 60

Query: 61  EEHGGSGDGAASPLAVTVFFGANDARLPDRYGAFQHVPLHEYKQNLISIVSFFKKKWPAT 120
           EE       AASPLAVTVFFGANDA LPDRYGAFQHVPLHEYKQNLISIV+FFKKKWPAT
Sbjct: 61  EER-----EAASPLAVTVFFGANDACLPDRYGAFQHVPLHEYKQNLISIVTFFKKKWPAT 120

Query: 121 RILLITPPPIDEEGRLQNPYVSNPLNEPERTNNAAGDYAKACIAVATECGVSVIDIWTKM 180
           RILLITPPPIDEEGRLQNPYV NPLNEPERTN+AAG YA ACIAVA ECGVSVIDIWTKM
Sbjct: 121 RILLITPPPIDEEGRLQNPYVPNPLNEPERTNDAAGAYANACIAVAKECGVSVIDIWTKM 180

Query: 181 QQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLKEEGLSPEMLRADLPLFSEIDSKNPLK 240
           QQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRL+EEGLSPE L ADLPLFSE+DS NPLK
Sbjct: 181 QQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPETLPADLPLFSEMDSTNPLK 240

Query: 241 AFEKL 246
           AFEKL
Sbjct: 241 AFEKL 240

BLAST of HG10016021 vs. ExPASy TrEMBL
Match: A0A6J1ED78 (GDSL esterase/lipase At5g45920 OS=Cucurbita moschata OX=3662 GN=LOC111433110 PE=3 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 2.8e-124
Identity = 217/245 (88.57%), Postives = 231/245 (94.29%), Query Frame = 0

Query: 1   MRPKIYLFGDSITEFSFADGGWGSALANHFSRRADVVVRGYSGYNTRWALKVIERVFPAS 60
           MRP+IYLFGDSITEFSFADGGWG+ALANHFSRRADVVVRGYSGYNTRWALKV+ERVFPAS
Sbjct: 1   MRPRIYLFGDSITEFSFADGGWGAALANHFSRRADVVVRGYSGYNTRWALKVLERVFPAS 60

Query: 61  EEHGGSGDGAASPLAVTVFFGANDARLPDRYGAFQHVPLHEYKQNLISIVSFFKKKWPAT 120
           EEH G+GD    PLA+TVFFGANDA LPDRYGAFQHVPL EYKQNLISIVSF KK+WPAT
Sbjct: 61  EEHSGAGD----PLALTVFFGANDACLPDRYGAFQHVPLDEYKQNLISIVSFLKKQWPAT 120

Query: 121 RILLITPPPIDEEGRLQNPYVSNPLNEPERTNNAAGDYAKACIAVATECGVSVIDIWTKM 180
           RILLITPPPIDEEGRLQNPY+ NPLNEPERTNNAAGDYAKACIAVA ECGVSVIDIWTKM
Sbjct: 121 RILLITPPPIDEEGRLQNPYLENPLNEPERTNNAAGDYAKACIAVAAECGVSVIDIWTKM 180

Query: 181 QQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLKEEGLSPEMLRADLPLFSEIDSKNPLK 240
           QQ+PGWEKACLSDGLHL+RNGNTIVFEEVV++LKEEGLSPEML ADLPL SE+D  +PLK
Sbjct: 181 QQLPGWEKACLSDGLHLSRNGNTIVFEEVVQKLKEEGLSPEMLPADLPLLSEMDYNDPLK 240

Query: 241 AFEKL 246
           AFE+L
Sbjct: 241 AFEEL 241

BLAST of HG10016021 vs. ExPASy TrEMBL
Match: A0A1S3B057 (GDSL esterase/lipase At5g45920 OS=Cucumis melo OX=3656 GN=LOC103484466 PE=4 SV=1)

HSP 1 Score: 452.6 bits (1163), Expect = 1.0e-123
Identity = 221/245 (90.20%), Postives = 227/245 (92.65%), Query Frame = 0

Query: 1   MRPKIYLFGDSITEFSFADGGWGSALANHFSRRADVVVRGYSGYNTRWALKVIERVFPAS 60
           MRPKIYLFGDSITEFSFADGGWGSAL NHF+R+ADVVVRGYSGYNTRWALKVIERVFP S
Sbjct: 1   MRPKIYLFGDSITEFSFADGGWGSALTNHFTRKADVVVRGYSGYNTRWALKVIERVFPPS 60

Query: 61  EEHGGSGDGAASPLAVTVFFGANDARLPDRYGAFQHVPLHEYKQNLISIVSFFKKKWPAT 120
           EE       AASPLAVTVFFGANDA LPDRYGAFQHVPLHEYKQNLISIV+F KKKWPAT
Sbjct: 61  EER-----EAASPLAVTVFFGANDACLPDRYGAFQHVPLHEYKQNLISIVTFLKKKWPAT 120

Query: 121 RILLITPPPIDEEGRLQNPYVSNPLNEPERTNNAAGDYAKACIAVATECGVSVIDIWTKM 180
           RILLITPPPIDEEGRLQNPYV NPLNEPERTN+AAG YA ACIAVA ECGVSVIDIWTKM
Sbjct: 121 RILLITPPPIDEEGRLQNPYVPNPLNEPERTNDAAGAYANACIAVAKECGVSVIDIWTKM 180

Query: 181 QQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLKEEGLSPEMLRADLPLFSEIDSKNPLK 240
           QQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRL+EEGLSPE L ADLPLFSE+DS NPLK
Sbjct: 181 QQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPETLPADLPLFSEMDSTNPLK 240

Query: 241 AFEKL 246
           AFEKL
Sbjct: 241 AFEKL 240

BLAST of HG10016021 vs. ExPASy TrEMBL
Match: A0A0A0KI83 (SGNH_hydro domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G518920 PE=4 SV=1)

HSP 1 Score: 451.4 bits (1160), Expect = 2.3e-123
Identity = 221/245 (90.20%), Postives = 227/245 (92.65%), Query Frame = 0

Query: 1   MRPKIYLFGDSITEFSFADGGWGSALANHFSRRADVVVRGYSGYNTRWALKVIERVFPAS 60
           MRPKIYLFGDSITEFSFADGGWGSAL NHF+R+ADVVVRGYSGYNTRWALKVIERVFP S
Sbjct: 1   MRPKIYLFGDSITEFSFADGGWGSALTNHFTRKADVVVRGYSGYNTRWALKVIERVFPPS 60

Query: 61  EEHGGSGDGAASPLAVTVFFGANDARLPDRYGAFQHVPLHEYKQNLISIVSFFKKKWPAT 120
           EE       AASPLAVTVFFGANDA LPDRYGAFQHVPLHEYKQNLISIVSFFKKKWPAT
Sbjct: 61  EER-----EAASPLAVTVFFGANDACLPDRYGAFQHVPLHEYKQNLISIVSFFKKKWPAT 120

Query: 121 RILLITPPPIDEEGRLQNPYVSNPLNEPERTNNAAGDYAKACIAVATECGVSVIDIWTKM 180
           RILLITPPPIDEEGRLQNPYVSNP+NEPERTN+AAG YAKACIAVA ECGVSVIDIWTKM
Sbjct: 121 RILLITPPPIDEEGRLQNPYVSNPVNEPERTNDAAGAYAKACIAVAKECGVSVIDIWTKM 180

Query: 181 QQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLKEEGLSPEMLRADLPLFSEIDSKNPLK 240
           QQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRL+EEGLSP  L ADLPL SEID  NPL+
Sbjct: 181 QQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPVTLPADLPLISEIDFNNPLQ 240

Query: 241 AFEKL 246
           AFEKL
Sbjct: 241 AFEKL 240

BLAST of HG10016021 vs. TAIR 10
Match: AT5G45920.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 347.1 bits (889), Expect = 1.2e-95
Identity = 163/244 (66.80%), Postives = 193/244 (79.10%), Query Frame = 0

Query: 1   MRPKIYLFGDSITEFSFADGGWGSALANHFSRRADVVVRGYSGYNTRWALKVIERVFPAS 60
           MR KI+LFGDSITE SF+DGGWG++LA+   R+AD+V+RGYSGYNTRWALKV+ERVFP +
Sbjct: 2   MRRKIFLFGDSITEESFSDGGWGASLADLLRRKADMVLRGYSGYNTRWALKVVERVFPVA 61

Query: 61  EEHGGSGDGAASPLAVTVFFGANDARLPDRYGAFQHVPLHEYKQNLISIVSFFKKKWPAT 120
           EE GG      SP AVTVFFGANDA LP+R   FQHVPLHEYKQNL SIVSF K +WP T
Sbjct: 62  EEDGGD-----SPAAVTVFFGANDACLPERCSGFQHVPLHEYKQNLRSIVSFLKNRWPQT 121

Query: 121 RILLITPPPIDEEGRLQNPYVSNPLNEPERTNNAAGDYAKACIAVATECGVSVIDIWTKM 180
            I+LITPPPIDEE RL+ PY+ N    PERTN  AG YAKACIAVA EC +SV D+W+KM
Sbjct: 122 AIILITPPPIDEEARLRYPYIENTTGLPERTNEVAGLYAKACIAVAEECQISVTDLWSKM 181

Query: 181 QQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLKEEGLSPEMLRADLPLFSEIDSKNPLK 240
           QQ+P W+  CL DGLHL+R GN +VFEEV K+LKEEG+  E L  DLPL  ++D K+PLK
Sbjct: 182 QQIPNWQTECLWDGLHLSRVGNKVVFEEVAKKLKEEGIGAEDLAVDLPLIEDVDPKDPLK 240

Query: 241 AFEK 245
           +F++
Sbjct: 242 SFDE 240

BLAST of HG10016021 vs. TAIR 10
Match: AT5G62930.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 257.3 bits (656), Expect = 1.2e-68
Identity = 129/247 (52.23%), Postives = 162/247 (65.59%), Query Frame = 0

Query: 1   MRPKIYLFGDSITEFSFADGGWGSALANHFSRRADVVVRGYSGYNTRWALKVIERVFPAS 60
           MRP+I LFGDSIT  SF  GGWGSALA+ +SR+ADVVVRGY GYNTRWAL ++  +FP  
Sbjct: 1   MRPEIVLFGDSITAQSFRSGGWGSALADAYSRKADVVVRGYGGYNTRWALFLLHHIFPLG 60

Query: 61  EEHGGSGDGAASPLAVTVFFGANDARLPDRYGAFQHVPLHEYKQNLISIVSFFKKKWPAT 120
                    ++ P+A T+FFGANDA L  R    QHVP+ EY  N+  IV   KK  P  
Sbjct: 61  --------SSSPPVATTIFFGANDAALKGRTSDRQHVPVEEYTDNVRKIVQHLKKCSPTM 120

Query: 121 RILLITPPPIDEEGR---LQNPYVSNPLNEPERTNNAAGDYAKACIAVATECGVSVIDIW 180
            I+LITPPPIDE GR    ++ Y    + EPERTN   G YA+ C+A+A E G+  +++W
Sbjct: 121 LIVLITPPPIDEAGRQSYAESIYGEKAMKEPERTNETTGVYAQHCVALAEELGLRCVNLW 180

Query: 181 TKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLKEEGLSPEMLRADLPLFSEIDSKN 240
           +KMQ+   W+K  LSDGLHLT  GN +VF+EV +  +E  LSPE +  D P  S ID KN
Sbjct: 181 SKMQETNDWQKKYLSDGLHLTPEGNGVVFDEVSRVFREAWLSPEEMPFDFPHHSHIDGKN 239

Query: 241 PLKAFEK 245
           P KAFE+
Sbjct: 241 PSKAFEE 239

BLAST of HG10016021 vs. TAIR 10
Match: AT3G11210.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 172.2 bits (435), Expect = 5.3e-43
Identity = 86/229 (37.55%), Postives = 132/229 (57.64%), Query Frame = 0

Query: 2   RPKIYLFGDSITEFSFADGGWGSALANHFSRRADVVVRGYSGYNTRWALKVIERVFPASE 61
           RP+I LFG SI + SF  GGWG+ L+  ++R+AD+++RGY G+N+  AL+V+++VFP   
Sbjct: 6   RPQIVLFGSSIVQMSFGHGGWGAILSEVYARKADIILRGYYGWNSSRALEVVDQVFPK-- 65

Query: 62  EHGGSGDGAASPLAVTVFFGANDARLPDRYGAFQHVPLHEYKQNLISIVSFFKKKWPATR 121
                 D A  P  V V+FG ND+  P   G   HVPL EY  N+  I    +     TR
Sbjct: 66  ------DAAVQPSLVIVYFGGNDSMAPHSSGLGPHVPLTEYVDNMKKIALHLQSLSDFTR 125

Query: 122 ILLITPPPIDEEGRLQN--PYVSNPLNEPERTNNAAGDYAKACIAVATECGVSVIDIWTK 181
           I+ ++ PP+DE    QN  PY    L+E  RTN+    Y+ AC+ +  E G+ V+D+++ 
Sbjct: 126 IIFLSSPPVDEAKVRQNQSPY----LSEVIRTNDLCKTYSDACVELCQELGLEVVDLFST 185

Query: 182 MQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLKEEGLSPEMLRADLP 229
            Q+   W+  C +DG+HL+  G+ IV  E+++ +KE    P +    +P
Sbjct: 186 FQKADDWKTVCFTDGIHLSAQGSKIVAGEILRVVKEAEWHPSLHWKSMP 222

BLAST of HG10016021 vs. TAIR 10
Match: AT2G38180.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 165.6 bits (418), Expect = 4.9e-41
Identity = 88/238 (36.97%), Postives = 131/238 (55.04%), Query Frame = 0

Query: 1   MRPKIYLFGDSITEFSFADGGWGSALANHFSRRADVVVRGYSGYNTRWALKVIERVFPAS 60
           +RP+I LFG SI ++SF D GWG+ LA+ +SR AD+++RGY+G+N+R+ALKV+ +VFP  
Sbjct: 5   VRPQIVLFGSSIVQYSFTDRGWGATLADLYSRTADIILRGYAGWNSRFALKVLHQVFPK- 64

Query: 61  EEHGGSGDGAASPLAVTVFFGANDARLPDRYGAFQHVPLHEYKQNLISIVSFFKKKWPAT 120
                  D    P  V V+FG ND+  P   G   HVPL E+ +N+  I          T
Sbjct: 65  -------DAVIQPSLVIVYFGGNDSTHPHPSGHGPHVPLSEFIENMRKIGEHLLSLSDKT 124

Query: 121 RILLITPPPIDEEGRLQNPYVSNPLNEPERTNNAAGDYAKACIAVATECGVSVIDIWTKM 180
           R++ +TPPP++E+   Q   V     +  R+N     YA+  + +  E  V  IDIWT +
Sbjct: 125 RVIFLTPPPMNEK---QIEIVFGDAIK-GRSNELCRPYAEELLNLCREINVKGIDIWTAI 184

Query: 181 QQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLKEEGLSPEMLRADLPLFSEIDSKNP 239
           QQ   W  +C +DG+H T   + IV +E++K L+     P +    LP+    D   P
Sbjct: 185 QQQDDWLNSCFTDGIHFTAKASEIVVKEILKVLRGADWKPSLYWKSLPVEFPFDFDAP 230

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038882981.15.1e-13394.29GDSL esterase/lipase At5g45920 [Benincasa hispida] >XP_038882982.1 GDSL esterase... [more]
XP_022978753.11.1e-12489.39GDSL esterase/lipase At5g45920 [Cucurbita maxima][more]
TYK13191.14.4e-12490.61GDSL esterase/lipase [Cucumis melo var. makuwa][more]
XP_022925816.15.7e-12488.57GDSL esterase/lipase At5g45920 [Cucurbita moschata] >KAG7034633.1 GDSL esterase/... [more]
XP_023544567.11.3e-12388.57GDSL esterase/lipase At5g45920 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q6NMR91.7e-9466.80GDSL esterase/lipase At5g45920 OS=Arabidopsis thaliana OX=3702 GN=At5g45920 PE=2... [more]
Q9FM041.8e-6752.23GDSL esterase/lipase At5g62930 OS=Arabidopsis thaliana OX=3702 GN=At5g62930 PE=2... [more]
Q9DB293.6e-4442.02Isoamyl acetate-hydrolyzing esterase 1 homolog OS=Mus musculus OX=10090 GN=Iah1 ... [more]
Q711G31.0e-4342.44Isoamyl acetate-hydrolyzing esterase 1 homolog OS=Rattus norvegicus OX=10116 GN=... [more]
Q3SZ161.8e-4342.02Isoamyl acetate-hydrolyzing esterase 1 homolog OS=Bos taurus OX=9913 GN=IAH1 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1IU695.6e-12589.39GDSL esterase/lipase At5g45920 OS=Cucurbita maxima OX=3661 GN=LOC111478618 PE=3 ... [more]
A0A5D3CMZ12.1e-12490.61GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255... [more]
A0A6J1ED782.8e-12488.57GDSL esterase/lipase At5g45920 OS=Cucurbita moschata OX=3662 GN=LOC111433110 PE=... [more]
A0A1S3B0571.0e-12390.20GDSL esterase/lipase At5g45920 OS=Cucumis melo OX=3656 GN=LOC103484466 PE=4 SV=1[more]
A0A0A0KI832.3e-12390.20SGNH_hydro domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G518920 ... [more]
Match NameE-valueIdentityDescription
AT5G45920.11.2e-9566.80SGNH hydrolase-type esterase superfamily protein [more]
AT5G62930.11.2e-6852.23SGNH hydrolase-type esterase superfamily protein [more]
AT3G11210.15.3e-4337.55SGNH hydrolase-type esterase superfamily protein [more]
AT2G38180.14.9e-4136.97SGNH hydrolase-type esterase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036514SGNH hydrolase superfamilyGENE3D3.40.50.1110SGNH hydrolasecoord: 2..242
e-value: 1.5E-72
score: 245.7
IPR013830SGNH hydrolase-type esterase domainPFAMPF13472Lipase_GDSL_2coord: 7..202
e-value: 2.2E-26
score: 93.3
IPR045136Isoamyl acetate-hydrolyzing esterase Iah1-likePANTHERPTHR14209ISOAMYL ACETATE-HYDROLYZING ESTERASE 1coord: 1..243
NoneNo IPR availablePANTHERPTHR14209:SF26ISOAMYL ACETATE-HYDROLYZING ESTERASE 1 HOMOLOGcoord: 1..243
NoneNo IPR availableCDDcd01838Isoamyl_acetate_hydrolase_likecoord: 4..211
e-value: 2.82811E-97
score: 280.678
NoneNo IPR availableSUPERFAMILY52266SGNH hydrolasecoord: 3..215

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10016021.1HG10016021.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016788 hydrolase activity, acting on ester bonds