HG10011602 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10011602
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionAlpha-farnesene synthase
LocationChr01: 7884188 .. 7888824 (-)
RNA-Seq ExpressionHG10011602
SyntenyHG10011602
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACCAAAATCATTGCTATTGGGTCAATGTTAGATGACATTTATGATGTTTATGGAACATTTGAAGAACTCAAACTCTTGACCCTTGCACTTGAGAGGTGGGTTTTCCTTCCCTTATTATTATTATTAGAGAAATTCGATAGATCCCACAAATGGGGTCTATCACTAATAACAGTTAAATTTTTTATTTTTTATATTTGGAAACAAAATATATATATATATATAAAAAGTATCTAGCATGATAGGTGAGAAATTAAACTACAAATTAAACAACATAAAAAACTAAAGCCAATCTTTGTTTTAGAAAAAAAAGTCGAATAATATAAAAATACTCCGGAATTTTATGATATATTTCAGTAAGGATGTCAAAATTTTTATCCTTTTAACATTGAAATTTATTTCAAAAAAAATACCATGGATGAATTTTTTTTTTTTTTTGTTAAAATAAAATATAGAAATGTTGTAATAAAAAATATAGAAATTTATTTGACAACTATGCATAAAAATTAAAAGGCATATATGGTATTATTTAAAACTATTTAATTAAAACGATTGTTTTCAAATATAGAAAATGGGTCAACTTATTTACAAATATAGTAAAATGTCACCGTCTATTATTGATATATGTTTATCACTGTCCATTACTGATAGAGAGTGACATTTTGCTATGTTTAAAAAAATTTTCAGCAATTTTTTATTTAAAAATTACTATTATTATAAAAAATTAACAAATACAAAAAATGTCAAACTATTTACAGAAAAGAGCAAAAAAACACTAATAGACATTGATAGACTTCTATCAGCATCTATCAGTGATAAACTTCTAAACATTTTGCTATTTTGTGTAAATAGTTTCCCTTATTTTTCTATTTTTAAAAATTCATTTTTATATTTTTCTTTTTCTTTTTTTTTCTAAAAAAATTATTTCACATTCTCTTATCCAAATGTTTTAAGTGGGTCTTTAAATAGTAATATTATATATATATATCCTCCACCCATATATAAATAAAAATCCTTTATTTTTTTTAATTACTATTTATATATGAAAAATTATAACAAATAGAAAAAATGTCAAACTATTTACAGAAAATAACAAAAAAACACTAATAGACATTGATAGACTTCTATCAGCATCTATTAGTGATAGACTTCTATTATTTGTATCACTGATAAACTCGGATAGACTTCTATCCGCGTCTATCACAACTATCTAAAAAATTTGTTATTTTGTGTAAATAGTTTCCCTTATTTTTCTATTTTTAAAAATTCATTTTTATATATTTCTTTTTCTTTTCTTTTCTTTTGTAAAAAAACTATTTCACATTCTTTTATCCAAATGTTTTAAGTGGGTCTTTAAATAGTAATATATATATATAATATATATATAATATATATATATATATATATATCCTCCACCCGTATATAAATAAAAATCCTTTAGTTTTTTTTTAATTAAACCCTTTCACATTCTCAAACTATATTGGACTAAATCTAAAATTTATGACTTTATTGTTAATTGTTATCGTAATTAAGAACCAAAATTTAAAAATTATAGAATAATTTTAATAAAATAAATTTTTAAAAAGAGTTTGAAGTTAAATGAACTCATTAAATGGACAAATAACAATAACAATAAAAATAAAAACAAAAAGTTAGATTATGAAAAAGATTGAATGTAAAAAGTTTTAAGTTTGGAGAATTTTTAAAAATAGAAAAATAAGGAAAATTATTTACACAAAATAGTAGAAATTTTAAATAGTTGTGATAGACGATGATAGATGTCTATTAGGGTCTATTAGTGATAGAAATGATAGAAGTCTATCACCGAATATGAAGAAGTCTTCAATGTCTATTAATATTATTTTTACTATTTTCGATAAATAATTTGATATTTTTTTAATAATGAAAATTTCCCAAAAAGAGAGTTTTTGGTTAATTTTGTCTTAAAAATTTATGAATGGTTAGAATTCGGTAATTAATTCTTAAGAACATTTTAATGTATTATAAATTTGTAAATTTAGCATTAATTTTAATGTAGGTCAATTAAATTAGAAACATAATGAAGTATGGAGGAACATGCAAAACCAACAGAGTTTGGTTGGTAAAATTCTATTTATGGGAACTAGTCCTTTTTTAAAAAACTTTTTAAATTATTCATAATTAATTATCCGATTATTGTAACAGTTTTAAAAGCTCGGTAAAAAAAAAAAAAAACGACTGTTTTTAGAAAGTACATAGGCGTTGAGCATTTAATTCACTCATACTTTTTTTTTTTTCAAAGGAATTCACTCATAATTATGTTCACATTTTAATCCAATAATAAATTTTAGAAAAATTACTATAAACAGAAAAAATATCAAACTATTTACAAATATAAAAAAATTTCACTAATTGTGACATAATTCTATCACTTTCTATCACTAAAGCAAATGAATTAATTCTATAGCTGATAGACTGTAAAATTTTTCTATATTTATAAATAGTTTGACTCATTTTTCTATATTTAAAAAGAGTCCTAAATTTTACATAGTCACGATTTTATTGGAATGTTTTTTTAAAAAAAAAAAATAATTAATTCAACTTTCAAGTTTTGAGTTATATTTTTTCCGTAAAAAAAAAATATCAAATAATGGATCTCTTAAATTTTGCAGATGGGACAAGAGTGAAACAGACCAACTCCCAAATTATATGAAGATGTACTACGAGGCTTTATTGGATATTTTTGAAGAGATTGAACAAGAAATGAGCCAGCAGAAAACAACACCATATTGCATCCATCACATGAAAGAAGCAGTATGAAATTGATAAAAACAAGATAAGAAAAAAAATAATCAAATACAAACCTAATAATCTGTCTCATAATCATTTAATTTTTTTTAAAAAAATGATAATCATTTACCTTTTTTATTATTTCTCAAAAATATTCTTATTTTCTACCATTTCTTCACGGTAGCTTTTTATATTTTTAAAGGAAATAATTGAAATCTTAACAAAAAAAATTATAAAACATAAACAAGTTTTTAACCCACCATTTGAAAATTATTTATTTTTAAATTTATGTTTGTTTTCTCACGGCTGTATTGATTTTCTTTTTTCATCTTTTCTAAGGAAAGATTGGAATTCATAAAGCATGACTAAGAAATGTATAATAAAACAAAGAAAATCAGTAGAGGTATTGTTTTCAAAAATATTATTTTTTAAAAACAAAAGATAGAAACCTAAATAACTCTCAAACATGGCCTAAAAGATTTTGCTTGAATTTTAAAATATATGTAAATTTTGATAACAAAATAAATAGCTTTATTGGTGAAAGTTGGGTTTATAGGTTTAATTTTAGAAAACCAAAAACAAAAAAAATTAAGACTCCATTTGATAAATTTTTTCCAAAATTGTGCTAATTTTTTCTTGCAATTTCTTTAAAATGGGGTTTACAAATTTTTTTTAAAGAAATATTTGAATTCATAGCAAAATTCAAAAATCACAAACAAGTTCTTAAAATTTACTTTGGAAATAGCTCTAAAATGTAGATGACAAAATCAACAAATCCATAGGTGAACATAGTATTAAATAACTACCTAATAAAGACTAAATTATTATCAAATGAAGTTGTGATAAATTAATTCATCTATTTTGTGTAGAGATGACATGTTTTGAGACACAATAATAATATTAATAATAATAAGGAAAATTTTACAGATAAAAAAATGTCAAACAATTTACAGAAAATAATAAAAAAATACTTATAGACATTGATAGACTTCTATCAGCATCTATCAATGATAGACTTCTATAATTTCTATTACTGATAGACCCTGATAAACTTCTATCAATGTCTATCACAACTATCTAAAAATTTTACTATTTTGTGTAAATAATTTCTCTTATTTTTTTTATTTTGAAAAATTCCCCTAATAATAATATCATTCAACACTAATTAGGATTATAAATTTTGTAGACAAAAGAGCTTGGAAGGGTATTTCTGGTAGAGGCAAAATGGTGCAAGGAAGGATACACACCAACTGTGGAAGAATACTTGGATATTGCTTTGATATCTTTTGGCCACAAATTGCTTATGGTAACAGCTCTTTTCGGAATGAGAGATTTGGCCACAGATCAGGCTGTTCAATGGGTCATCTCCATGCCTAATATTCTCAAGGCCTCCACAGTAATTTGTAGGCTCATGAACGACATCGTTTCGCATAAGGTTAGCTACCTAGCTAGCTAGCTCCATAATTATATCTCTACAAAATAATTAGTTTACGTAACTAAAATATATACGTTCATCAATCGTCTTTGATTTATGGTTTGAACAAGAACGAGGTCATGTAGTTTCTGCCATTGAATGCTACATGGAACAGAATTGTGCGTCGTATTGCAACGTCCTGAATGCATTACACAAACAAATAGATGATTCTTGGAAGGATATGGTTGAGAATTATTGTGTTACCACCACACATGATCAGGTTCCAATGGCTGTTCTTATGAGAGTTTTGAACCTCACAAGACTATTTAATGTGATATACAAAGATGGAGATGGGTATACACAATCTCAAGGCTCCACAAACCCATATCTCTTTGCTTGTAGACTCTGTCCCACTCTAGGGCTTTTCAATTTAAAAGCTTTGTCATACATATAA

mRNA sequence

ATGACCAAAATCATTGCTATTGGGTCAATGTTAGATGACATTTATGATGTTTATGGAACATTTGAAGAACTCAAACTCTTGACCCTTGCACTTGAGAGATGGGACAAGAGTGAAACAGACCAACTCCCAAATTATATGAAGATGTACTACGAGGCTTTATTGGATATTTTTGAAGAGATTGAACAAGAAATGAGCCAGCAGAAAACAACACCATATTGCATCCATCACATGAAAGAAGCAACAAAAGAGCTTGGAAGGGTATTTCTGGTAGAGGCAAAATGGTGCAAGGAAGGATACACACCAACTGTGGAAGAATACTTGGATATTGCTTTGATATCTTTTGGCCACAAATTGCTTATGGTAACAGCTCTTTTCGGAATGAGAGATTTGGCCACAGATCAGGCTGTTCAATGGGTCATCTCCATGCCTAATATTCTCAAGGCCTCCACAGTAATTTGTAGGCTCATGAACGACATCGTTTCGCATAAGAATTGTGCGTCGTATTGCAACGTCCTGAATGCATTACACAAACAAATAGATGATTCTTGGAAGGATATGGTTGAGAATTATTGTGTTACCACCACACATGATCAGGTTCCAATGGCTGTTCTTATGAGAGTTTTGAACCTCACAAGACTATTTAATGTGATATACAAAGATGGAGATGGGTATACACAATCTCAAGGCTCCACAAACCCATATCTCTTTGCTTGTAGACTCTGTCCCACTCTAGGGCTTTTCAATTTAAAAGCTTTGTCATACATATAA

Coding sequence (CDS)

ATGACCAAAATCATTGCTATTGGGTCAATGTTAGATGACATTTATGATGTTTATGGAACATTTGAAGAACTCAAACTCTTGACCCTTGCACTTGAGAGATGGGACAAGAGTGAAACAGACCAACTCCCAAATTATATGAAGATGTACTACGAGGCTTTATTGGATATTTTTGAAGAGATTGAACAAGAAATGAGCCAGCAGAAAACAACACCATATTGCATCCATCACATGAAAGAAGCAACAAAAGAGCTTGGAAGGGTATTTCTGGTAGAGGCAAAATGGTGCAAGGAAGGATACACACCAACTGTGGAAGAATACTTGGATATTGCTTTGATATCTTTTGGCCACAAATTGCTTATGGTAACAGCTCTTTTCGGAATGAGAGATTTGGCCACAGATCAGGCTGTTCAATGGGTCATCTCCATGCCTAATATTCTCAAGGCCTCCACAGTAATTTGTAGGCTCATGAACGACATCGTTTCGCATAAGAATTGTGCGTCGTATTGCAACGTCCTGAATGCATTACACAAACAAATAGATGATTCTTGGAAGGATATGGTTGAGAATTATTGTGTTACCACCACACATGATCAGGTTCCAATGGCTGTTCTTATGAGAGTTTTGAACCTCACAAGACTATTTAATGTGATATACAAAGATGGAGATGGGTATACACAATCTCAAGGCTCCACAAACCCATATCTCTTTGCTTGTAGACTCTGTCCCACTCTAGGGCTTTTCAATTTAAAAGCTTTGTCATACATATAA

Protein sequence

MTKIIAIGSMLDDIYDVYGTFEELKLLTLALERWDKSETDQLPNYMKMYYEALLDIFEEIEQEMSQQKTTPYCIHHMKEATKELGRVFLVEAKWCKEGYTPTVEEYLDIALISFGHKLLMVTALFGMRDLATDQAVQWVISMPNILKASTVICRLMNDIVSHKNCASYCNVLNALHKQIDDSWKDMVENYCVTTTHDQVPMAVLMRVLNLTRLFNVIYKDGDGYTQSQGSTNPYLFACRLCPTLGLFNLKALSYI
Homology
BLAST of HG10011602 vs. NCBI nr
Match: XP_038903296.1 (alpha-farnesene synthase-like [Benincasa hispida] >XP_038903297.1 alpha-farnesene synthase-like [Benincasa hispida])

HSP 1 Score: 400.2 bits (1027), Expect = 1.3e-107
Identity = 199/253 (78.66%), Postives = 215/253 (84.98%), Query Frame = 0

Query: 1   MTKIIAIGSMLDDIYDVYGTFEELKLLTLALERWDKSETDQLPNYMKMYYEALLDIFEEI 60
           MTKIIAIGSMLDDIYDVYGTFEEL+LLT ALERW+KSET+QLPNY+KMYY+ALLD+F++I
Sbjct: 295 MTKIIAIGSMLDDIYDVYGTFEELELLTHALERWEKSETEQLPNYLKMYYDALLDVFDKI 354

Query: 61  EQEMSQQKTTPYCIHHMKEATKELGRVFLVEAKWCKEGYTPTVEEYLDIALISFGHKLLM 120
           E EMS Q+TT YCIHHMKEATKELGRVFLVEA WCKEGYTPTVEEYLDIALISFGHKLLM
Sbjct: 355 EHEMSNQETTTYCIHHMKEATKELGRVFLVEANWCKEGYTPTVEEYLDIALISFGHKLLM 414

Query: 121 VTALFGMRDLATDQAVQWVISMPNILKASTVICRLMNDIVSHK----------------- 180
           VTAL GM DL T QAVQWV SMPNILKASTVICR MND+VSHK                 
Sbjct: 415 VTALLGMGDLGTQQAVQWVTSMPNILKASTVICRFMNDMVSHKFEQERGHVASAIECYME 474

Query: 181 -NCASYCNVLNALHKQIDDSWKDMVENYCVTTTHDQVPMAVLMRVLNLTRLFNVIYKDGD 236
            N AS  + +NALHKQIDDSWKD+VENYCV TT+D+VP AVL RVLNLTRLFNVIYKDGD
Sbjct: 475 QNSASEYDAINALHKQIDDSWKDIVENYCVVTTNDEVPRAVLRRVLNLTRLFNVIYKDGD 534

BLAST of HG10011602 vs. NCBI nr
Match: KGN56187.1 (hypothetical protein Csa_010016 [Cucumis sativus])

HSP 1 Score: 382.5 bits (981), Expect = 2.9e-102
Identity = 198/256 (77.34%), Postives = 207/256 (80.86%), Query Frame = 0

Query: 1   MTKIIAIGSMLDDIYDVYGTFEELKLLTLALERWDKSETDQLPNYMKMYYEALLDIFEEI 60
           MTK+IAIGSMLDDIYDVYGTFEELKLLTLALERWDKSET QLPNYMKMYYEALLD+FEEI
Sbjct: 296 MTKVIAIGSMLDDIYDVYGTFEELKLLTLALERWDKSETKQLPNYMKMYYEALLDVFEEI 355

Query: 61  EQEMSQQ--KTTPYCIHHMKEATKELGRVFLVEAKWCKEGYTPTVEEYLDIALISFGHKL 120
           EQEMSQ+  +TTPYCIHHMKEATKELGRVFLVEA WCKEGYTP VEEYLDIALISFGHKL
Sbjct: 356 EQEMSQKETETTPYCIHHMKEATKELGRVFLVEATWCKEGYTPKVEEYLDIALISFGHKL 415

Query: 121 LMVTALFGM-RDLATDQAVQWVISMPNILKASTVICRLMNDIVSHK-------------- 180
           LMVTAL GM   +AT Q VQW+ SMPNILKAS VICRLMNDIVSHK              
Sbjct: 416 LMVTALLGMGSHMATQQIVQWITSMPNILKASAVICRLMNDIVSHKFEQERGHVASAIEC 475

Query: 181 ----NCASYCNVLNALHKQIDDSWKDMVENYCVTTTHDQVPMAVLMRVLNLTRLFNVIYK 236
               N  S    L AL KQIDD WKDMVENYC   T D+VP  VLMRVLNLTRLFNVIYK
Sbjct: 476 YMEQNHLSEYEALIALRKQIDDLWKDMVENYCAVITEDEVPRGVLMRVLNLTRLFNVIYK 535

BLAST of HG10011602 vs. NCBI nr
Match: NP_001267674.1 (alpha-farnesene synthase-like [Cucumis sativus] >AAU05951.1 E,E-alpha-farnesene synthase [Cucumis sativus])

HSP 1 Score: 382.5 bits (981), Expect = 2.9e-102
Identity = 198/256 (77.34%), Postives = 207/256 (80.86%), Query Frame = 0

Query: 1   MTKIIAIGSMLDDIYDVYGTFEELKLLTLALERWDKSETDQLPNYMKMYYEALLDIFEEI 60
           MTK+IAIGSMLDDIYDVYGTFEELKLLTLALERWDKSET QLPNYMKMYYEALLD+FEEI
Sbjct: 296 MTKVIAIGSMLDDIYDVYGTFEELKLLTLALERWDKSETKQLPNYMKMYYEALLDVFEEI 355

Query: 61  EQEMSQQ--KTTPYCIHHMKEATKELGRVFLVEAKWCKEGYTPTVEEYLDIALISFGHKL 120
           EQEMSQ+  +TTPYCIHHMKEATKELGRVFLVEA WCKEGYTP VEEYLDIALISFGHKL
Sbjct: 356 EQEMSQKETETTPYCIHHMKEATKELGRVFLVEATWCKEGYTPKVEEYLDIALISFGHKL 415

Query: 121 LMVTALFGM-RDLATDQAVQWVISMPNILKASTVICRLMNDIVSHK-------------- 180
           LMVTAL GM   +AT Q VQW+ SMPNILKAS VICRLMNDIVSHK              
Sbjct: 416 LMVTALLGMGSHMATQQIVQWITSMPNILKASAVICRLMNDIVSHKFEQERGHVASAIEC 475

Query: 181 ----NCASYCNVLNALHKQIDDSWKDMVENYCVTTTHDQVPMAVLMRVLNLTRLFNVIYK 236
               N  S    L AL KQIDD WKDMVENYC   T D+VP  VLMRVLNLTRLFNVIYK
Sbjct: 476 YMEQNHLSEYEALIALRKQIDDLWKDMVENYCAVITEDEVPRGVLMRVLNLTRLFNVIYK 535

BLAST of HG10011602 vs. NCBI nr
Match: NP_001284384.1 (alpha-farnesene synthase [Cucumis melo] >B2KSJ6.1 RecName: Full=Alpha-farnesene synthase; Short=CmTpsDul [Cucumis melo] >ABX83201.1 terpene synthase [Cucumis melo])

HSP 1 Score: 381.3 bits (978), Expect = 6.4e-102
Identity = 195/254 (76.77%), Postives = 209/254 (82.28%), Query Frame = 0

Query: 1   MTKIIAIGSMLDDIYDVYGTFEELKLLTLALERWDKSETDQLPNYMKMYYEALLDIFEEI 60
           MTKIIAIGS+LDDIYDVYGTFEELKLLTLALERWDKSET +LP YMKMYYEALLD+FEEI
Sbjct: 297 MTKIIAIGSVLDDIYDVYGTFEELKLLTLALERWDKSETKKLPKYMKMYYEALLDVFEEI 356

Query: 61  EQEMSQQKTTPYCIHHMKEATKELGRVFLVEAKWCKEGYTPTVEEYLDIALISFGHKLLM 120
           EQEMSQ++TTPYCIH MKEATKELGRVFLVEAKWCKEGYTPTVEEYLDIALISFGHKLLM
Sbjct: 357 EQEMSQKETTPYCIHQMKEATKELGRVFLVEAKWCKEGYTPTVEEYLDIALISFGHKLLM 416

Query: 121 VTALFGM-RDLATDQAVQWVISMPNILKASTVICRLMNDIVSHK---------------- 180
           VTAL GM   +AT Q VQW+ SMPNILKAS +ICRLMNDIVSHK                
Sbjct: 417 VTALLGMGSTIATQQIVQWITSMPNILKASAIICRLMNDIVSHKFEQERGHVASAIECYM 476

Query: 181 --NCASYCNVLNALHKQIDDSWKDMVENYCVTTTHDQVPMAVLMRVLNLTRLFNVIYKDG 236
             N  S  +VL  L KQID+ WKDMVENYCV  T ++VP  VLMRVLNLTRLFNVIYKDG
Sbjct: 477 EQNYMSEHDVLIILGKQIDEFWKDMVENYCVVITEEEVPRGVLMRVLNLTRLFNVIYKDG 536

BLAST of HG10011602 vs. NCBI nr
Match: XP_031282885.1 (LOW QUALITY PROTEIN: (-)-germacrene D synthase-like [Pistacia vera])

HSP 1 Score: 221.9 bits (564), Expect = 6.5e-54
Identity = 116/262 (44.27%), Postives = 166/262 (63.36%), Query Frame = 0

Query: 1   MTKIIAIGSMLDDIYDVYGTFEELKLLTLALERWDKSETDQLPNYMKMYYEALLDIFEEI 60
           +TK+ AI S++DD YD YGT +EL+L T A+ERWD S  DQLP+YM++ Y  LLD++ EI
Sbjct: 300 LTKVFAITSIIDDTYDAYGTVDELELFTSAIERWDISAIDQLPDYMQIIYATLLDVYNEI 359

Query: 61  EQEMSQQKTTPYCIHHMKEATKELGRVFLVEAKWCKEGYTPTVEEYLDIALISFGHKLLM 120
           E EM+  K   Y +H+ KE  K L R + +EAKWC E Y PT++EY+ +AL++  +++L 
Sbjct: 360 ETEMT-SKGQLYRLHYAKEVMKTLVRNYFIEAKWCHENYVPTMDEYMSVALVTGAYQMLT 419

Query: 121 VTALFGMRDLATDQAVQWVISMPNILKASTVICRLMNDIVSHK------NCASYCNVL-- 180
            T+  GM D+AT +A +W++S P +L+A++VICRLM+DI SHK      + AS       
Sbjct: 420 TTSSVGMGDVATIEAFEWLVSNPKLLRAASVICRLMDDIASHKFEQKRGHVASGVECFMK 479

Query: 181 ----------NALHKQIDDSWKDMVENYCVTTTHDQVPMAVLMRVLNLTRLFNVIYKDGD 240
                     N   KQI D WKD+ E     TT   +PM +L+R+LN TR+ +VIYKDGD
Sbjct: 480 QYDAIEEEPYNEFXKQITDVWKDVNEELLRPTT---LPMRLLVRILNFTRVIDVIYKDGD 539

Query: 241 GYTQSQGSTNPYLFACRLCPTL 245
           GY+ +Q     Y+ +  + P L
Sbjct: 540 GYSDAQVYLKGYITSLFIDPVL 557

BLAST of HG10011602 vs. ExPASy Swiss-Prot
Match: B2KSJ6 (Alpha-farnesene synthase OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 8.4e-105
Identity = 195/254 (76.77%), Postives = 209/254 (82.28%), Query Frame = 0

Query: 1   MTKIIAIGSMLDDIYDVYGTFEELKLLTLALERWDKSETDQLPNYMKMYYEALLDIFEEI 60
           MTKIIAIGS+LDDIYDVYGTFEELKLLTLALERWDKSET +LP YMKMYYEALLD+FEEI
Sbjct: 297 MTKIIAIGSVLDDIYDVYGTFEELKLLTLALERWDKSETKKLPKYMKMYYEALLDVFEEI 356

Query: 61  EQEMSQQKTTPYCIHHMKEATKELGRVFLVEAKWCKEGYTPTVEEYLDIALISFGHKLLM 120
           EQEMSQ++TTPYCIH MKEATKELGRVFLVEAKWCKEGYTPTVEEYLDIALISFGHKLLM
Sbjct: 357 EQEMSQKETTPYCIHQMKEATKELGRVFLVEAKWCKEGYTPTVEEYLDIALISFGHKLLM 416

Query: 121 VTALFGM-RDLATDQAVQWVISMPNILKASTVICRLMNDIVSHK---------------- 180
           VTAL GM   +AT Q VQW+ SMPNILKAS +ICRLMNDIVSHK                
Sbjct: 417 VTALLGMGSTIATQQIVQWITSMPNILKASAIICRLMNDIVSHKFEQERGHVASAIECYM 476

Query: 181 --NCASYCNVLNALHKQIDDSWKDMVENYCVTTTHDQVPMAVLMRVLNLTRLFNVIYKDG 236
             N  S  +VL  L KQID+ WKDMVENYCV  T ++VP  VLMRVLNLTRLFNVIYKDG
Sbjct: 477 EQNYMSEHDVLIILGKQIDEFWKDMVENYCVVITEEEVPRGVLMRVLNLTRLFNVIYKDG 536

BLAST of HG10011602 vs. ExPASy Swiss-Prot
Match: Q6Q3H3 ((-)-germacrene D synthase OS=Vitis vinifera OX=29760 GN=VIT_19s0014g04930 PE=1 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 2.0e-50
Identity = 104/243 (42.80%), Postives = 154/243 (63.37%), Query Frame = 0

Query: 1   MTKIIAIGSMLDDIYDVYGTFEELKLLTLALERWDKSETDQLPNYMKMYYEALLDIFEEI 60
           +TK+IA+ S++DDIYDVYGT EEL+L T A+ERWD S  DQLP YM++ Y+ALL ++ EI
Sbjct: 299 LTKVIAMTSIIDDIYDVYGTLEELELFTEAVERWDISAIDQLPEYMRVCYQALLYVYSEI 358

Query: 61  EQEMSQQKTTPYCIHHMKEATKELGRVFLVEAKWCKEGYTPTVEEYLDIALISFGHKLLM 120
           E+EM+++  + Y +++ KEA K   R +  EAKW +    PT+EEY+ +AL++  + +L 
Sbjct: 359 EEEMAKEGRS-YRLYYAKEAMKNQVRAYYEEAKWLQVQQIPTMEEYMPVALVTSAYSMLA 418

Query: 121 VTALFGMRDLATDQAVQWVISMPNILKASTVICRLMNDIVSHK----------------- 180
            T+  GM D  T ++  W+ S P I++AS ++CRLM+D+V HK                 
Sbjct: 419 TTSFVGMGDAVTKESFDWIFSKPKIVRASAIVCRLMDDMVFHKFEQKRGHVASAVECYMK 478

Query: 181 -NCASYCNVLNALHKQIDDSWKDMVENYCVTTTHDQVPMAVLMRVLNLTRLFNVIYKDGD 226
            + AS     N   + + ++WKD+ E   + T    VPM +LMRVLNL R+ +VIYK+ D
Sbjct: 479 QHGASEQETPNEFPQPVREAWKDINEECLIPTA---VPMPILMRVLNLARVIDVIYKNED 537

BLAST of HG10011602 vs. ExPASy Swiss-Prot
Match: B9S9Z3 (Alpha-copaene synthase OS=Ricinus communis OX=3988 GN=TPS1 PE=1 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 4.5e-50
Identity = 106/246 (43.09%), Postives = 154/246 (62.60%), Query Frame = 0

Query: 1   MTKIIAIGSMLDDIYDVYGTFEELKLLTLALERWDKSETDQLPNYMKMYYEALLDIFEEI 60
           MTKIIAI S+LDD YD Y T EEL++LT A+ERWD    D LP YMK+ Y  LLDI+ E 
Sbjct: 299 MTKIIAITSLLDDTYDNYATGEELEILTEAIERWDIKAKDALPEYMKIIYTTLLDIYNEY 358

Query: 61  EQEMS-QQKTTPYCIHHMKEATKELGRVFLVEAKWCKEGYTPTVEEYLDIALISFGHKLL 120
           E+ ++ ++K+  Y +++ KE  K + R +L E +W    YTPT+EEY+  AL++    +L
Sbjct: 359 EENIAKEEKSLLYSVYYAKEVMKRVVRAYLAEVRWRDNCYTPTMEEYMQSALLTTCSPML 418

Query: 121 MVTALFGMRDLATDQAVQWVISMPNILKASTVICRLMNDIVSHK------------NC-- 180
            + +  G++++AT +A +W    P I++AS+++CRLM+DIVSH+             C  
Sbjct: 419 AIASFLGLKEIATKEAYEWASEDPKIIRASSIVCRLMDDIVSHEFEQTRKHVASGVECYI 478

Query: 181 ----ASYCNVLNALHKQIDDSWKDMVENYCVTTTHDQVPMAVLMRVLNLTRLFNVIYKDG 228
               AS   V+    K++ ++WKD+ E  C+  T   VPM +L RV+NLTR  +VIYKD 
Sbjct: 479 KQYGASEEEVIKLFRKEVTNAWKDLNEE-CLNPT--PVPMPMLERVVNLTRAIDVIYKDD 538

BLAST of HG10011602 vs. ExPASy Swiss-Prot
Match: B9SCB6 (Probable terpene synthase 2 OS=Ricinus communis OX=3988 GN=TPS2 PE=3 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 1.5e-48
Identity = 103/243 (42.39%), Postives = 151/243 (62.14%), Query Frame = 0

Query: 1   MTKIIAIGSMLDDIYDVYGTFEELKLLTLALERWDKSETDQLPNYMKMYYEALLDIFEEI 60
           +TK+I + S++DDIYD YGT EEL + T A+ RWD    DQLP YMK+ Y+ALL+++EEI
Sbjct: 294 VTKVINVVSVIDDIYDAYGTLEELVVFTDAINRWDIDCIDQLPEYMKVCYKALLNVYEEI 353

Query: 61  EQEMSQQKTTPYCIHHMKEATKELGRVFLVEAKWCKEGYTPTVEEYLDIALISFGHKLLM 120
           E+ +S+Q  + Y +H+ KEA K+L + +LVEA W  + Y PT++EY+ IAL+S  + LL 
Sbjct: 354 ERALSEQGRS-YRLHYAKEAMKKLVQAYLVEANWMNKNYVPTMDEYMSIALVSCAYPLLT 413

Query: 121 VTALFGMRDLATDQAVQWVISMPNILKASTVICRLMNDIVSH------------------ 180
           VT+  GM D+AT +   W  + P I++ +++ICRLM+DIVSH                  
Sbjct: 414 VTSFVGMGDIATKEVFDWASNDPKIVRVASIICRLMDDIVSHEFEQKRGHIASSVECYMK 473

Query: 181 KNCASYCNVLNALHKQIDDSWKDMVENYCVTTTHDQVPMAVLMRVLNLTRLFNVIYKDGD 226
           +N  S     +  +KQI D+WKD+ E +      + VPM    RV+N  R+ + +YKD D
Sbjct: 474 QNGVSEEATRDEFNKQIVDAWKDINEEH---LQPNYVPMPFRTRVVNSARIMDYLYKDDD 532

BLAST of HG10011602 vs. ExPASy Swiss-Prot
Match: Q8SA63 (Beta-caryophyllene synthase OS=Artemisia annua OX=35608 GN=QHS1 PE=1 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 1.6e-47
Identity = 95/243 (39.09%), Postives = 151/243 (62.14%), Query Frame = 0

Query: 1   MTKIIAIGSMLDDIYDVYGTFEELKLLTLALERWDKSETDQLPNYMKMYYEALLDIFEEI 60
           + K+I++ ++LDD YD YGT+EELK+ T A++RW  +  D LP Y+K+ Y+ +LDI+ E+
Sbjct: 290 LAKVISLATVLDDTYDAYGTYEELKIFTEAIQRWSITCIDMLPEYLKLLYQGVLDIYIEM 349

Query: 61  EQEMSQQKTTPYCIHHMKEATKELGRVFLVEAKWCKEGYTPTVEEYLDIALISFGHKLLM 120
           E+ M ++    + + + KE+ KE  R +++EAKW  EGY PT EE++ +A +S G+ +L 
Sbjct: 350 EEIMGKEGKAHH-LSYAKESMKEFIRSYMMEAKWANEGYVPTAEEHMSVAFVSSGYSMLA 409

Query: 121 VTALFGMRDLATDQAVQWVISMPNILKASTVICRLMNDIVSHK----------NCASYC- 180
            T   GM D+ TD+A +W ++ P I+KAS  I RLM+DI S K          +  SY  
Sbjct: 410 TTCFVGMGDIVTDEAFKWALTKPPIIKASCAIARLMDDIHSQKEEKERIHVASSVESYMK 469

Query: 181 -------NVLNALHKQIDDSWKDMVENYCVTTTHDQVPMAVLMRVLNLTRLFNVIYKDGD 226
                  +VL   +K+I+D+WKD+     V      +PM ++MRV+NL ++ +V+YK  D
Sbjct: 470 QYDVTEEHVLKVFNKKIEDAWKDITRESLV---RKDIPMPLMMRVINLAQVMDVLYKHKD 528

BLAST of HG10011602 vs. ExPASy TrEMBL
Match: Q66PX9 (E,E-alpha-farnesene synthase OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 382.5 bits (981), Expect = 1.4e-102
Identity = 198/256 (77.34%), Postives = 207/256 (80.86%), Query Frame = 0

Query: 1   MTKIIAIGSMLDDIYDVYGTFEELKLLTLALERWDKSETDQLPNYMKMYYEALLDIFEEI 60
           MTK+IAIGSMLDDIYDVYGTFEELKLLTLALERWDKSET QLPNYMKMYYEALLD+FEEI
Sbjct: 296 MTKVIAIGSMLDDIYDVYGTFEELKLLTLALERWDKSETKQLPNYMKMYYEALLDVFEEI 355

Query: 61  EQEMSQQ--KTTPYCIHHMKEATKELGRVFLVEAKWCKEGYTPTVEEYLDIALISFGHKL 120
           EQEMSQ+  +TTPYCIHHMKEATKELGRVFLVEA WCKEGYTP VEEYLDIALISFGHKL
Sbjct: 356 EQEMSQKETETTPYCIHHMKEATKELGRVFLVEATWCKEGYTPKVEEYLDIALISFGHKL 415

Query: 121 LMVTALFGM-RDLATDQAVQWVISMPNILKASTVICRLMNDIVSHK-------------- 180
           LMVTAL GM   +AT Q VQW+ SMPNILKAS VICRLMNDIVSHK              
Sbjct: 416 LMVTALLGMGSHMATQQIVQWITSMPNILKASAVICRLMNDIVSHKFEQERGHVASAIEC 475

Query: 181 ----NCASYCNVLNALHKQIDDSWKDMVENYCVTTTHDQVPMAVLMRVLNLTRLFNVIYK 236
               N  S    L AL KQIDD WKDMVENYC   T D+VP  VLMRVLNLTRLFNVIYK
Sbjct: 476 YMEQNHLSEYEALIALRKQIDDLWKDMVENYCAVITEDEVPRGVLMRVLNLTRLFNVIYK 535

BLAST of HG10011602 vs. ExPASy TrEMBL
Match: A0A0A0L6G4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G095040 PE=4 SV=1)

HSP 1 Score: 382.5 bits (981), Expect = 1.4e-102
Identity = 198/256 (77.34%), Postives = 207/256 (80.86%), Query Frame = 0

Query: 1   MTKIIAIGSMLDDIYDVYGTFEELKLLTLALERWDKSETDQLPNYMKMYYEALLDIFEEI 60
           MTK+IAIGSMLDDIYDVYGTFEELKLLTLALERWDKSET QLPNYMKMYYEALLD+FEEI
Sbjct: 296 MTKVIAIGSMLDDIYDVYGTFEELKLLTLALERWDKSETKQLPNYMKMYYEALLDVFEEI 355

Query: 61  EQEMSQQ--KTTPYCIHHMKEATKELGRVFLVEAKWCKEGYTPTVEEYLDIALISFGHKL 120
           EQEMSQ+  +TTPYCIHHMKEATKELGRVFLVEA WCKEGYTP VEEYLDIALISFGHKL
Sbjct: 356 EQEMSQKETETTPYCIHHMKEATKELGRVFLVEATWCKEGYTPKVEEYLDIALISFGHKL 415

Query: 121 LMVTALFGM-RDLATDQAVQWVISMPNILKASTVICRLMNDIVSHK-------------- 180
           LMVTAL GM   +AT Q VQW+ SMPNILKAS VICRLMNDIVSHK              
Sbjct: 416 LMVTALLGMGSHMATQQIVQWITSMPNILKASAVICRLMNDIVSHKFEQERGHVASAIEC 475

Query: 181 ----NCASYCNVLNALHKQIDDSWKDMVENYCVTTTHDQVPMAVLMRVLNLTRLFNVIYK 236
               N  S    L AL KQIDD WKDMVENYC   T D+VP  VLMRVLNLTRLFNVIYK
Sbjct: 476 YMEQNHLSEYEALIALRKQIDDLWKDMVENYCAVITEDEVPRGVLMRVLNLTRLFNVIYK 535

BLAST of HG10011602 vs. ExPASy TrEMBL
Match: A0A1S2XAE3 (alpha-farnesene synthase OS=Cucumis melo OX=3656 GN=TPS PE=4 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 3.1e-102
Identity = 195/254 (76.77%), Postives = 209/254 (82.28%), Query Frame = 0

Query: 1   MTKIIAIGSMLDDIYDVYGTFEELKLLTLALERWDKSETDQLPNYMKMYYEALLDIFEEI 60
           MTKIIAIGS+LDDIYDVYGTFEELKLLTLALERWDKSET +LP YMKMYYEALLD+FEEI
Sbjct: 297 MTKIIAIGSVLDDIYDVYGTFEELKLLTLALERWDKSETKKLPKYMKMYYEALLDVFEEI 356

Query: 61  EQEMSQQKTTPYCIHHMKEATKELGRVFLVEAKWCKEGYTPTVEEYLDIALISFGHKLLM 120
           EQEMSQ++TTPYCIH MKEATKELGRVFLVEAKWCKEGYTPTVEEYLDIALISFGHKLLM
Sbjct: 357 EQEMSQKETTPYCIHQMKEATKELGRVFLVEAKWCKEGYTPTVEEYLDIALISFGHKLLM 416

Query: 121 VTALFGM-RDLATDQAVQWVISMPNILKASTVICRLMNDIVSHK---------------- 180
           VTAL GM   +AT Q VQW+ SMPNILKAS +ICRLMNDIVSHK                
Sbjct: 417 VTALLGMGSTIATQQIVQWITSMPNILKASAIICRLMNDIVSHKFEQERGHVASAIECYM 476

Query: 181 --NCASYCNVLNALHKQIDDSWKDMVENYCVTTTHDQVPMAVLMRVLNLTRLFNVIYKDG 236
             N  S  +VL  L KQID+ WKDMVENYCV  T ++VP  VLMRVLNLTRLFNVIYKDG
Sbjct: 477 EQNYMSEHDVLIILGKQIDEFWKDMVENYCVVITEEEVPRGVLMRVLNLTRLFNVIYKDG 536

BLAST of HG10011602 vs. ExPASy TrEMBL
Match: A0A2I4H149 ((-)-germacrene D synthase-like OS=Juglans regia OX=51240 GN=LOC109012609 PE=4 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 7.0e-54
Identity = 110/247 (44.53%), Postives = 162/247 (65.59%), Query Frame = 0

Query: 1   MTKIIAIGSMLDDIYDVYGTFEELKLLTLALERWDKSETDQLPNYMKMYYEALLDIFEEI 60
           MTK+I + S+LDD YD YG++ ELKL T A+ERWD S  D LP YMK+ Y+ALLD+++EI
Sbjct: 320 MTKVIGMTSILDDTYDAYGSYAELKLFTEAIERWDVSAIDILPEYMKLIYKALLDVYDEI 379

Query: 61  EQEMSQQKTTPYCIHHMKEATKELGRVFLVEAKWCKEGYTPTVEEYLDIALISFGHKLLM 120
           E E ++    P+C+H+ KE+ K+L + + +EAKWC EGY PT+EEY+  A+ +  +++L 
Sbjct: 380 EAETAKD-GRPFCVHYAKESMKKLIQAYFIEAKWCNEGYAPTMEEYMSNAMTTSAYQMLA 439

Query: 121 VTALFGMRDLATDQAVQWVISMPNILKASTVICRLMNDIVSHKNCASYCNVLNA------ 180
            T+  GM ++A ++  +WV + P IL+AST+ICRLM+DI SHK   S  + ++A      
Sbjct: 440 PTSFLGMGNIADEEVFKWVFNDPKILRASTIICRLMDDIKSHKFEQSRAHAVSAVECYMK 499

Query: 181 -------------LHKQIDDSWKDMVENYCVTTTHD-QVPMAVLMRVLNLTRLFNVIYKD 228
                        L K+I ++WKD+ E   +  T D  VPM +L RVLNL R+ +++Y+D
Sbjct: 500 QYGVSAEEEVYKLLGKEIVNAWKDINEELLMNRTADHHVPMPILERVLNLARVIDLVYED 559

BLAST of HG10011602 vs. ExPASy TrEMBL
Match: A0A6M9BJ01 ((-)-germacrene D synthase (Fragment) OS=Zanthoxylum armatum OX=67938 PE=2 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 1.2e-53
Identity = 111/245 (45.31%), Postives = 160/245 (65.31%), Query Frame = 0

Query: 1   MTKIIAIGSMLDDIYDVYGTFEELKLLTLALERWDKSETDQLPNYMKMYYEALLDIFEEI 60
           MTK+IA+ S++DDIYD YG  EELKLLTLA+ERWD S  DQLP YMK++Y A LD++ EI
Sbjct: 238 MTKVIAMTSVMDDIYDAYGQIEELKLLTLAIERWDISAIDQLPEYMKLFYGAFLDVYREI 297

Query: 61  EQEMSQQKTTPYCIHHMKEATKELGRVFLVEAKWCKEGYTPTVEEYLDIALISFGHKLLM 120
           E +M+ Q    Y +H+ KEA K L + + +EA+WC + Y P ++EY+ +AL++  + +L 
Sbjct: 298 EIDMADQGNL-YRLHYAKEAMKILVKNYFLEAEWCHQKYVPPMDEYMPVALVTCAYPMLA 357

Query: 121 VTALFGMRDLATDQAVQWVISMPNILKASTVICRLMNDIVSHK----------------- 180
            T+  GM D+AT+ A +W+ S P ++ AS ++CR M+DIVSHK                 
Sbjct: 358 TTSFLGMGDIATEAAFKWLFSNPKMVTASEIVCRFMDDIVSHKFEQSRGHVASSIECYMK 417

Query: 181 -NCASYCNVLNALHKQIDDSWKDMVENYCVTTTHDQVPMAVLMRVLNLTRLFNVIYKDGD 228
            N A+     +   KQ+ ++WKD+ E  C+  T   VPM +LMR+LNLTR+ +V+YKD D
Sbjct: 418 QNGATEEEACSEFRKQVSNAWKDINEE-CLRPT--AVPMPLLMRILNLTRVIDVLYKDAD 477

BLAST of HG10011602 vs. TAIR 10
Match: AT3G32030.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 132.1 bits (331), Expect = 6.3e-31
Identity = 82/249 (32.93%), Postives = 126/249 (50.60%), Query Frame = 0

Query: 1   MTKIIAIGSMLDDIYDVYGTFEELKLLTLALERWDKSETDQLPNYMKMYYEALLDIFEEI 60
           + K+  I   L+D+ D Y T+ E K L  +L+RWD    D+LPNY ++    +L+   EI
Sbjct: 345 IAKMTIIVVALNDVCDSYATYPEAKSLIDSLQRWDIEAIDELPNYSRIVLRLILETIGEI 404

Query: 61  EQEMSQQKTTPYCIHHMKEATKELGRVFLVEAKWCKEGYTPTVEEYLDIALISFGHKLLM 120
           E+EM  +  +   + H  + TK LGR +L  +KW  EGY PT +EY+++  ++ G     
Sbjct: 405 EREMKPRGRSA-SVQHTIDETKSLGRAYLALSKWASEGYMPTFDEYMEVGEVTGGMDDFA 464

Query: 121 VTALFGMRDLATDQAVQWVISMPNILKASTVICRLMNDIVSHKNCASYCNVLNA------ 180
           + +   M D       +W  S P IL+A +V+ R+ NDIV+++   S   V+N       
Sbjct: 465 LYSFIAMEDCDEKPLYEWFDSKPKILQALSVLYRINNDIVTYEREMSKGEVVNGVNSYMN 524

Query: 181 ------------LHKQIDDSWKDMVENYCVTTTHDQVPMAVLMRVLNLTRLFNVIYKDG- 231
                       L K   D++K ++E     T    VP  VL+R LNL RLF+V  K G 
Sbjct: 525 QHGVTKEEAVEELRKMARDNYKIVMEELLTIT---DVPRPVLVRCLNLARLFDVFCKHGN 584

BLAST of HG10011602 vs. TAIR 10
Match: AT1G70080.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 129.8 bits (325), Expect = 3.1e-30
Identity = 76/251 (30.28%), Postives = 128/251 (51.00%), Query Frame = 0

Query: 1   MTKIIAIGSMLDDIYDVYGTFEELKLLTLALERWDKSETDQLPNYMKMYYEALLDIFEEI 60
           + K I +  ++D+ +DVYGT +E+K L  A+ERWD    D LP+Y+K+ +    D+F+E+
Sbjct: 352 LAKSIILVDIVDNTFDVYGTIDEVKSLVQAIERWDSDAVDVLPDYLKVVFRTTFDLFKEL 411

Query: 61  EQEMSQQKTTPYCIHHMKEATKELGRVFLVEAKWCKEGYTPTVEEYLDIALISFGHKLLM 120
           E+ +S +  + + + +  E  + L + +L EA+W   G+ P+ EEY+++ + S   ++L+
Sbjct: 412 EEYVSSEARS-FTMQYAYEQLRILMKGYLQEAEWSNRGHLPSHEEYIEVGVASTAGEVLL 471

Query: 121 VTALFGMRDLATDQAVQWVISMPNILKASTVICRLMNDIVSHK---NCASYCNVLNALHK 180
                 M D A     +W+ S P +  A  V  RL +DI ++K        CN +N   K
Sbjct: 472 AMTFIPMGDAAGVGVYEWLRSRPKLTHALFVKSRLRDDIATYKEEMKRGDVCNGINCYTK 531

Query: 181 QIDDSWKDMVENYCVTTTHDQ-------------VPMAVLMRVLNLTRLFNVIYKDGDGY 236
           Q   S ++    +   T H               +P+ +L  VLN  RL +V YK GDGY
Sbjct: 532 QHKVSEEEACIEFEKKTNHMSKVMNEEFLKAAKFIPLHILRPVLNYGRLADVCYKYGDGY 591

BLAST of HG10011602 vs. TAIR 10
Match: AT3G14540.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 129.4 bits (324), Expect = 4.1e-30
Identity = 75/258 (29.07%), Postives = 134/258 (51.94%), Query Frame = 0

Query: 1   MTKIIAIGSMLDDIYDVYGTFEELKLLTLALERWDKSETDQLPNYMKMYYEALLDIFEEI 60
           + K I I  + DD YD + T  E  +LT   +R +    D+L  Y+++  E++ ++  EI
Sbjct: 342 VAKTIMIIVVADDTYDAHATIPEATVLTEYFQRLNIGADDKLSGYLRIVLESVFEVMGEI 401

Query: 61  EQEMSQQKTTPYCIHHMKEATKELGRVFLVEAKWCKEGYTPTVEEYLDIALISFGHKLLM 120
           EQEMS  K   Y +  + E  K + + +    +W ++G+ PT +EY+ + L++ G     
Sbjct: 402 EQEMS-PKGRSYSVKQVLERFKIIAKAYKQLTEWARKGHVPTFDEYMKVGLVTAGMGDYA 461

Query: 121 VTALFGMRDLATDQAVQWVISMPNILKASTVICRLMNDIVSHK---NCASYCNVLNALHK 180
                GM D+   +A +W+ S P ++ A  V+ R+ ND+ +++   N     N LN   K
Sbjct: 462 GYCFIGMEDINEKEAFEWLNSNPLLIDALNVLFRIANDVGTYETEINRGEVANGLNCYMK 521

Query: 181 QI----DDSWKDMVENYCVT---------TTHDQVPMAVLMRVLNLTRLFNVIYKDGDGY 240
           Q     +++ +++ + Y             +HD+VP  VL+R LN  RLF+VIY +GDGY
Sbjct: 522 QYGVTKEEASRELRKMYIYNKKVVVEEFMNSHDRVPRQVLLRCLNFARLFDVIYTEGDGY 581

Query: 241 TQSQGSTNPYLFACRLCP 243
           ++ +G    ++ +  + P
Sbjct: 582 SEPKGKIEHFMTSLYVHP 598

BLAST of HG10011602 vs. TAIR 10
Match: AT1G33750.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 129.0 bits (323), Expect = 5.3e-30
Identity = 73/246 (29.67%), Postives = 127/246 (51.63%), Query Frame = 0

Query: 2   TKIIAIGSMLDDIYDVYGTFEELKLLTLALERWDKSETDQLPNYMKMYYEALLDIFEEIE 61
           TK+  +  ++DD  D YGTF E++ L  +LERWD    D+LP+ +++  +++++  E+IE
Sbjct: 346 TKVSQVIVVMDDTCDAYGTFSEVRSLIDSLERWDPGAIDKLPSCLRIVIQSIVETMEDIE 405

Query: 62  QEMSQQKTTPYCIHHMKEATKELGRVFLVEAKWCKEGYTPTVEEYLDIALISFGHKLLMV 121
           +EM  +  +   +    E  K +GR +   +KW + G+ PT ++Y+++ L S G +   +
Sbjct: 406 REMKPRGRSS-SVQDTVEEIKIMGRAYAEISKWARAGHVPTFDDYIELGLDSSGIRCFAM 465

Query: 122 TALFGMRDLATDQAVQWVISMPNILKASTVICRLMNDIVSHKNCASYCNVLNALH----- 181
            +   M D   +Q   W  S P +L+A +VI RL NDI   +       V+N ++     
Sbjct: 466 YSFISMEDCEENQTNAWFKSKPKMLRALSVIFRLTNDIAGFEEEMRRGEVVNGVNCYVKQ 525

Query: 182 -------------KQIDDSWKDMVENYCVTTTHDQVPMAVLMRVLNLTRLFNVIYKDGDG 230
                        K I D++K M+E +    T   V   +L+R  N+ RL N+ Y++GD 
Sbjct: 526 HNVTKELAVREIKKMIRDNYKIMMEEF---LTIKSVSRPILVRCFNIVRLVNLYYEEGDN 585

BLAST of HG10011602 vs. TAIR 10
Match: AT3G14520.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 128.6 bits (322), Expect = 7.0e-30
Identity = 76/258 (29.46%), Postives = 136/258 (52.71%), Query Frame = 0

Query: 1   MTKIIAIGSMLDDIYDVYGTFEELKLLTLALERWDKSETDQLPNYMKMYYEALLDIFEEI 60
           + KII    ++DD YD + T  E+ +LT  L+R +    D+LP+Y++   E++ ++  EI
Sbjct: 345 VAKIIMTMVVVDDTYDAHATVPEVAVLTECLQRLNIGADDKLPDYLRTVLESVFEVMGEI 404

Query: 61  EQEMSQQKTTPYCIHHMKEATKELGRVFLVEAKWCKEGYTPTVEEYLDIALISFGHKLLM 120
           EQEM + K   Y +  + E  K + +      +W + G  P+ +EY+ + L++ G     
Sbjct: 405 EQEM-RPKGRSYGVKQVLERFKNVAKADKQLTEWARTGDVPSFDEYMKVGLVTAGMDGYA 464

Query: 121 VTALFGMRDLATDQAVQWVISMPNILKASTVICRLMNDIVSHK---NCASYCNVLNALHK 180
                GM D++  +A +W+ S P I++A  V+ RL ND+ +++   N     N LN   K
Sbjct: 465 GYCFIGMEDVSEKEAFEWLSSNPLIIQALNVMFRLANDVGTYETEINRGEVANGLNCYMK 524

Query: 181 QI----DDSWKDMVENYC---------VTTTHDQVPMAVLMRVLNLTRLFNVIYKDGDGY 240
           Q     +++ +++ + Y             +HD VP  VL+R LN  RLF+V+Y +GDGY
Sbjct: 525 QYGVTKEEASQELRKIYSNNKKVVMEEFMNSHDHVPRQVLLRCLNFARLFDVMYTEGDGY 584

Query: 241 TQSQGSTNPYLFACRLCP 243
           ++ +G    ++ +  + P
Sbjct: 585 SEPKGKIEHFMTSLYVHP 601

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038903296.11.3e-10778.66alpha-farnesene synthase-like [Benincasa hispida] >XP_038903297.1 alpha-farnesen... [more]
KGN56187.12.9e-10277.34hypothetical protein Csa_010016 [Cucumis sativus][more]
NP_001267674.12.9e-10277.34alpha-farnesene synthase-like [Cucumis sativus] >AAU05951.1 E,E-alpha-farnesene ... [more]
NP_001284384.16.4e-10276.77alpha-farnesene synthase [Cucumis melo] >B2KSJ6.1 RecName: Full=Alpha-farnesene ... [more]
XP_031282885.16.5e-5444.27LOW QUALITY PROTEIN: (-)-germacrene D synthase-like [Pistacia vera][more]
Match NameE-valueIdentityDescription
B2KSJ68.4e-10576.77Alpha-farnesene synthase OS=Cucumis melo OX=3656 PE=1 SV=1[more]
Q6Q3H32.0e-5042.80(-)-germacrene D synthase OS=Vitis vinifera OX=29760 GN=VIT_19s0014g04930 PE=1 S... [more]
B9S9Z34.5e-5043.09Alpha-copaene synthase OS=Ricinus communis OX=3988 GN=TPS1 PE=1 SV=1[more]
B9SCB61.5e-4842.39Probable terpene synthase 2 OS=Ricinus communis OX=3988 GN=TPS2 PE=3 SV=1[more]
Q8SA631.6e-4739.09Beta-caryophyllene synthase OS=Artemisia annua OX=35608 GN=QHS1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
Q66PX91.4e-10277.34E,E-alpha-farnesene synthase OS=Cucumis sativus OX=3659 PE=2 SV=1[more]
A0A0A0L6G41.4e-10277.34Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G095040 PE=4 SV=1[more]
A0A1S2XAE33.1e-10276.77alpha-farnesene synthase OS=Cucumis melo OX=3656 GN=TPS PE=4 SV=1[more]
A0A2I4H1497.0e-5444.53(-)-germacrene D synthase-like OS=Juglans regia OX=51240 GN=LOC109012609 PE=4 SV... [more]
A0A6M9BJ011.2e-5345.31(-)-germacrene D synthase (Fragment) OS=Zanthoxylum armatum OX=67938 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT3G32030.16.3e-3132.93Terpenoid cyclases/Protein prenyltransferases superfamily protein [more]
AT1G70080.13.1e-3030.28Terpenoid cyclases/Protein prenyltransferases superfamily protein [more]
AT3G14540.14.1e-3029.07Terpenoid cyclases/Protein prenyltransferases superfamily protein [more]
AT1G33750.15.3e-3029.67Terpenoid cyclases/Protein prenyltransferases superfamily protein [more]
AT3G14520.17.0e-3029.46Terpenoid cyclases/Protein prenyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 254..255
NoneNo IPR availablePANTHERPTHR31225:SF111TERPENE SYNTHASE 2-RELATEDcoord: 1..227
NoneNo IPR availablePANTHERPTHR31225OS04G0344100 PROTEIN-RELATEDcoord: 1..227
IPR005630Terpene synthase, metal-binding domainPFAMPF03936Terpene_synth_Ccoord: 1..166
e-value: 3.9E-57
score: 193.6
IPR008949Isoprenoid synthase domain superfamilyGENE3D1.10.600.10Farnesyl Diphosphate Synthasecoord: 1..240
e-value: 6.4E-74
score: 250.9
IPR008949Isoprenoid synthase domain superfamilySUPERFAMILY48576Terpenoid synthasescoord: 1..233

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10011602.1HG10011602.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016102 diterpenoid biosynthetic process
biological_process GO:0045338 farnesyl diphosphate metabolic process
biological_process GO:0051762 sesquiterpene biosynthetic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0052578 alpha-farnesene synthase activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0016829 lyase activity
molecular_function GO:0010333 terpene synthase activity