HG10011296 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10011296
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionCytosolic Fe-S cluster assembly factor NBP35
LocationChr01: 4527361 .. 4529682 (+)
RNA-Seq ExpressionHG10011296
SyntenyHG10011296
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAACAGCGAAATCCCTGAAAACGCTAATGAAAGTAATGGATTTCTTTCCTGTTCATTGTTTTTGTCATTTGTAGATTGTTCAACTTATTAGCCCTTTTTTGTTTTCTGTATTTCATTTTCATTGATGTTCTCTTTGTGGTGGCTTTTTCGATTTCAGATTGCCCAGGTCCTCAGTCGGAGTCGGCAGGGAAATCCGATGCCTGTCAAGGATGTCCCAACCAATCAATTTGTGCTACTGCGCCCAAAGGCCCTGACCCAGGTTCCCTTTCTCCTCCTCTGAATCTATTTGCTTTTTGTTCACATCTAAATTAGTTTAAAGAGATTGCTTTGTTGTTACATCGCATGAGCTTTTGGGACTGATTGGGGTTTATTCTGTTTAGATGGTTTTTGAAGCTAAAGTTAATGTCAATTGTCAGTTGATGAGATTACTATCTATATGGCATTATTTGTATTTTTTTTTTTCCTGATAATCTGGGTTTTGCTTCTTCATGGCTCACCCACTACTCTTTTGGGAGACTTTTCCTGTTCCAAAATAAGTAGTTAATCAAGTTGGATTTAACTTTGGTTATATACTTTCCGCTTTTGTCCAGTTTGATCTTTGGACTACGAAGATTTCCCCTTGGCTTATGTTTTATATAATTTTTGTGATCATAATTTTAGCATAAAACACTGCACTCATAAATTTGTAAAAATAGTCAGTTCATAAATTTGTAAAAATAGTCAGTTCGTAAATGGAAGGTTTTCACCAAGTATAGAATTTGTGTTAAGATTTTACAAACATGAATTTTACAGGACACTTTTTTAGAACTCAACGGAACAGTTGTACAACTTATAGAAATGATCCTGTTCGAGTAAGGTCATTTTCTAAAAGTACAGAAACCAAAATGAGTGGATGAGTGGATGTTTTGAAAGTACCATCAAAATAGACAGGTTAGAAATATAGGGAACAAAAAGAAGTTATTTAAGTTTTCCTAAAACATTGGAGGGTTGCACAAGACACCTTTTATTTGTATTTTTTATTCTCTCAAACCAAGCAAAGATGTAAGTTAAACTTTACAAGCTTTAGAAGACGTCTCTTATTAGTTGTTACTTGTTAGTTTACTGTACTACCAGTAAGTGAAATTGTGATGTGATGGGCAGAAGTACTTTTTGCTGGCTTCTAATATTGCCTTGTTTAGAATATACCTTAGTTCTCTATCTTCCTTTTATTTGGTATAGGGTTACTCTACAATTCTACATTTTGGCCAATTGCTTCTTGGTCGTAAAGGCATTAGGGTTTGGAACTGTTTGGTTTTTTGAATCCTATGTATGAATACACTAGTCGAATCAATTAACTGTTAGTGTGTTTATGCTCTATTTACCATTCTTTCCTCACCTTTTTCCAGACTTGGTTTCAATAGCAGAACGAATGGCAACGGTTAAGCACAAGATTCTTATTCTATCAGGTAAGGGTGGGGTCGGAAAGAGCACGTTCTCTACTCAGCTTGCATTTGCGCTAGCTGCCATGGATTTTCAGGTGGGTCTCCTCGATATCGATATTTGTGGTCCGAGTATCCCGAAGATGCTTGGTCTAGAAGGTCAAGATGTGCATCAGAGTAACCTTGGATGGTCCCCTGTTTACATAGAGTCCAACCTTGGAGTTATGTCAATTGGTTTCATGCTCCCTAACCCGGACGAAGCAGTCATTTGGAGAGGCCCTCGAAAAAATGGGCTCATTAAGCAGTTTCTCAAGGATGTGTATTGGGGAGAACTTGATTTTCTAGTTGTTGATGCTCCACCAGGGACTTCAGATGAGCACATATCAATCGTACAATGCCTTGAGGCTACTGGAATTGATGGTGCAATCATTGTCACCACACCACAACAAGTGTCTCTCATCGACGTGCGAAAAGAAGTGAGTTTTTGCAAAAAAGTAGGTGTAAAAGTTCTCGGAGTTGTAGAGAACATGAGCAGCTTGTGCCAACCACTATTAGATATGAAGTTTCTGAAGACGACAGAGGCAGGAGAGCAGGCAGACGTATCAGAATGGGTGGCAGAGTACATTCGGGAAAAAGCGCCAGAGCTACTAAACTTGATTACTTGTAGTGAAGTGTTCGATAGTAGCAGGGGTGGCGCTGTGAAAATGTGTAGGGAGATGGATGTGCCATTCCTGGGAAAAGTACCATTGGATCCACAGCTTTGCAAGGCAGCTGAAGAAGGAAGGTCCTGTTTTGGTGACAAAAAATGTGGGGTAAGTGCCTCAGCACTGAAGAAGATTATAGAGAAGTTGATACAAAATCAAGGGTTTTCAACAATGTCAATAGATGCTAAAGCGTAG

mRNA sequence

ATGGAGAACAGCGAAATCCCTGAAAACGCTAATGAAAATTGCCCAGGTCCTCAGTCGGAGTCGGCAGGGAAATCCGATGCCTGTCAAGGATGTCCCAACCAATCAATTTGTGCTACTGCGCCCAAAGGCCCTGACCCAGACTTGGTTTCAATAGCAGAACGAATGGCAACGGTTAAGCACAAGATTCTTATTCTATCAGGTAAGGGTGGGGTCGGAAAGAGCACGTTCTCTACTCAGCTTGCATTTGCGCTAGCTGCCATGGATTTTCAGGTGGGTCTCCTCGATATCGATATTTGTGGTCCGAGTATCCCGAAGATGCTTGGTCTAGAAGGTCAAGATGTGCATCAGAGTAACCTTGGATGGTCCCCTGTTTACATAGAGTCCAACCTTGGAGTTATGTCAATTGGTTTCATGCTCCCTAACCCGGACGAAGCAGTCATTTGGAGAGGCCCTCGAAAAAATGGGCTCATTAAGCAGTTTCTCAAGGATGTGTATTGGGGAGAACTTGATTTTCTAGTTGTTGATGCTCCACCAGGGACTTCAGATGAGCACATATCAATCGTACAATGCCTTGAGGCTACTGGAATTGATGGTGCAATCATTGTCACCACACCACAACAAGTGTCTCTCATCGACGTGCGAAAAGAAGTGAGTTTTTGCAAAAAAGTAGGTGTAAAAGTTCTCGGAGTTGTAGAGAACATGAGCAGCTTGTGCCAACCACTATTAGATATGAAGTTTCTGAAGACGACAGAGGCAGGAGAGCAGGCAGACGTATCAGAATGGGTGGCAGAGTACATTCGGGAAAAAGCGCCAGAGCTACTAAACTTGATTACTTGTAGTGAAGTGTTCGATAGTAGCAGGGGTGGCGCTGTGAAAATGTGTAGGGAGATGGATGTGCCATTCCTGGGAAAAGTACCATTGGATCCACAGCTTTGCAAGGCAGCTGAAGAAGGAAGGTCCTGTTTTGGTGACAAAAAATGTGGGGTAAGTGCCTCAGCACTGAAGAAGATTATAGAGAAGTTGATACAAAATCAAGGGTTTTCAACAATGTCAATAGATGCTAAAGCGTAG

Coding sequence (CDS)

ATGGAGAACAGCGAAATCCCTGAAAACGCTAATGAAAATTGCCCAGGTCCTCAGTCGGAGTCGGCAGGGAAATCCGATGCCTGTCAAGGATGTCCCAACCAATCAATTTGTGCTACTGCGCCCAAAGGCCCTGACCCAGACTTGGTTTCAATAGCAGAACGAATGGCAACGGTTAAGCACAAGATTCTTATTCTATCAGGTAAGGGTGGGGTCGGAAAGAGCACGTTCTCTACTCAGCTTGCATTTGCGCTAGCTGCCATGGATTTTCAGGTGGGTCTCCTCGATATCGATATTTGTGGTCCGAGTATCCCGAAGATGCTTGGTCTAGAAGGTCAAGATGTGCATCAGAGTAACCTTGGATGGTCCCCTGTTTACATAGAGTCCAACCTTGGAGTTATGTCAATTGGTTTCATGCTCCCTAACCCGGACGAAGCAGTCATTTGGAGAGGCCCTCGAAAAAATGGGCTCATTAAGCAGTTTCTCAAGGATGTGTATTGGGGAGAACTTGATTTTCTAGTTGTTGATGCTCCACCAGGGACTTCAGATGAGCACATATCAATCGTACAATGCCTTGAGGCTACTGGAATTGATGGTGCAATCATTGTCACCACACCACAACAAGTGTCTCTCATCGACGTGCGAAAAGAAGTGAGTTTTTGCAAAAAAGTAGGTGTAAAAGTTCTCGGAGTTGTAGAGAACATGAGCAGCTTGTGCCAACCACTATTAGATATGAAGTTTCTGAAGACGACAGAGGCAGGAGAGCAGGCAGACGTATCAGAATGGGTGGCAGAGTACATTCGGGAAAAAGCGCCAGAGCTACTAAACTTGATTACTTGTAGTGAAGTGTTCGATAGTAGCAGGGGTGGCGCTGTGAAAATGTGTAGGGAGATGGATGTGCCATTCCTGGGAAAAGTACCATTGGATCCACAGCTTTGCAAGGCAGCTGAAGAAGGAAGGTCCTGTTTTGGTGACAAAAAATGTGGGGTAAGTGCCTCAGCACTGAAGAAGATTATAGAGAAGTTGATACAAAATCAAGGGTTTTCAACAATGTCAATAGATGCTAAAGCGTAG

Protein sequence

MENSEIPENANENCPGPQSESAGKSDACQGCPNQSICATAPKGPDPDLVSIAERMATVKHKILILSGKGGVGKSTFSTQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLGWSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGTSDEHISIVQCLEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSSLCQPLLDMKFLKTTEAGEQADVSEWVAEYIREKAPELLNLITCSEVFDSSRGGAVKMCREMDVPFLGKVPLDPQLCKAAEEGRSCFGDKKCGVSASALKKIIEKLIQNQGFSTMSIDAKA
Homology
BLAST of HG10011296 vs. NCBI nr
Match: XP_038883029.1 (cytosolic Fe-S cluster assembly factor NBP35 [Benincasa hispida])

HSP 1 Score: 698.0 bits (1800), Expect = 4.3e-197
Identity = 343/356 (96.35%), Postives = 350/356 (98.31%), Query Frame = 0

Query: 1   MENSEIPENANENCPGPQSESAGKSDACQGCPNQSICATAPKGPDPDLVSIAERMATVKH 60
           MEN+EIPENANENCPGPQSESAGKSDACQGCPNQSICATAPKGPDPDLVSIAERMATVKH
Sbjct: 1   MENNEIPENANENCPGPQSESAGKSDACQGCPNQSICATAPKGPDPDLVSIAERMATVKH 60

Query: 61  KILILSGKGGVGKSTFSTQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLG 120
           KIL+LSGKGGVGKSTFS QLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLG
Sbjct: 61  KILVLSGKGGVGKSTFSAQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLG 120

Query: 121 WSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGT 180
           WSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGT
Sbjct: 121 WSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGT 180

Query: 181 SDEHISIVQCLEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSSLCQP 240
           SDEHISIVQCLEA+GIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSSLCQP
Sbjct: 181 SDEHISIVQCLEASGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSSLCQP 240

Query: 241 LLDMKFLKTTEAGEQADVSEWVAEYIREKAPELLNLITCSEVFDSSRGGAVKMCREMDVP 300
           LLDMKFLKTTEAGEQ DV+EWVAEYIREKAPELLN+I+CSEVFDSS GGA  MCREMDVP
Sbjct: 241 LLDMKFLKTTEAGEQTDVTEWVAEYIREKAPELLNVISCSEVFDSSGGGAANMCREMDVP 300

Query: 301 FLGKVPLDPQLCKAAEEGRSCFGDKKCGVSASALKKIIEKLIQNQGFSTMSIDAKA 357
           FLGKVPLDPQLCKAAEEGRSCFGD+KCGVSASALKKIIEKLIQNQGFSTMSID KA
Sbjct: 301 FLGKVPLDPQLCKAAEEGRSCFGDQKCGVSASALKKIIEKLIQNQGFSTMSIDDKA 356

BLAST of HG10011296 vs. NCBI nr
Match: KAE8649747.1 (hypothetical protein Csa_012684 [Cucumis sativus])

HSP 1 Score: 696.4 bits (1796), Expect = 1.3e-196
Identity = 343/356 (96.35%), Postives = 349/356 (98.03%), Query Frame = 0

Query: 1   MENSEIPENANENCPGPQSESAGKSDACQGCPNQSICATAPKGPDPDLVSIAERMATVKH 60
           MENSEIPENANENCPGP+SESAGKSDACQGCPNQSICATAPKGPDPDL+SIAERMATVKH
Sbjct: 25  MENSEIPENANENCPGPESESAGKSDACQGCPNQSICATAPKGPDPDLISIAERMATVKH 84

Query: 61  KILILSGKGGVGKSTFSTQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLG 120
           KIL+LSGKGGVGKSTFS QLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLG
Sbjct: 85  KILVLSGKGGVGKSTFSAQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLG 144

Query: 121 WSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGT 180
           WSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGT
Sbjct: 145 WSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGT 204

Query: 181 SDEHISIVQCLEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSSLCQP 240
           SDEHISIVQCL+ATGIDGAIIVTTPQQVSLIDVRKEVSFCK VGVKVLGVVENMSSLCQP
Sbjct: 205 SDEHISIVQCLKATGIDGAIIVTTPQQVSLIDVRKEVSFCKTVGVKVLGVVENMSSLCQP 264

Query: 241 LLDMKFLKTTEAGEQADVSEWVAEYIREKAPELLNLITCSEVFDSSRGGAVKMCREMDVP 300
           LLDMKFLKTTEAGEQ DV+EWV EYIREKAPELLNLIT SEVFDSS GGA KMCREMDVP
Sbjct: 265 LLDMKFLKTTEAGEQTDVTEWVGEYIREKAPELLNLITYSEVFDSSGGGAAKMCREMDVP 324

Query: 301 FLGKVPLDPQLCKAAEEGRSCFGDKKCGVSASALKKIIEKLIQNQGFSTMSIDAKA 357
           FLGKVPLDPQLCKAAEEGRSCFGD+KCGVSASALKKIIEKLIQNQGFSTMSIDAKA
Sbjct: 325 FLGKVPLDPQLCKAAEEGRSCFGDQKCGVSASALKKIIEKLIQNQGFSTMSIDAKA 380

BLAST of HG10011296 vs. NCBI nr
Match: XP_004144673.1 (cytosolic Fe-S cluster assembly factor NBP35 [Cucumis sativus] >XP_011653919.1 cytosolic Fe-S cluster assembly factor NBP35 [Cucumis sativus] >XP_031739512.1 cytosolic Fe-S cluster assembly factor NBP35 [Cucumis sativus] >XP_031739513.1 cytosolic Fe-S cluster assembly factor NBP35 [Cucumis sativus] >XP_031739514.1 cytosolic Fe-S cluster assembly factor NBP35 [Cucumis sativus])

HSP 1 Score: 696.4 bits (1796), Expect = 1.3e-196
Identity = 343/356 (96.35%), Postives = 349/356 (98.03%), Query Frame = 0

Query: 1   MENSEIPENANENCPGPQSESAGKSDACQGCPNQSICATAPKGPDPDLVSIAERMATVKH 60
           MENSEIPENANENCPGP+SESAGKSDACQGCPNQSICATAPKGPDPDL+SIAERMATVKH
Sbjct: 1   MENSEIPENANENCPGPESESAGKSDACQGCPNQSICATAPKGPDPDLISIAERMATVKH 60

Query: 61  KILILSGKGGVGKSTFSTQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLG 120
           KIL+LSGKGGVGKSTFS QLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLG
Sbjct: 61  KILVLSGKGGVGKSTFSAQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLG 120

Query: 121 WSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGT 180
           WSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGT
Sbjct: 121 WSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGT 180

Query: 181 SDEHISIVQCLEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSSLCQP 240
           SDEHISIVQCL+ATGIDGAIIVTTPQQVSLIDVRKEVSFCK VGVKVLGVVENMSSLCQP
Sbjct: 181 SDEHISIVQCLKATGIDGAIIVTTPQQVSLIDVRKEVSFCKTVGVKVLGVVENMSSLCQP 240

Query: 241 LLDMKFLKTTEAGEQADVSEWVAEYIREKAPELLNLITCSEVFDSSRGGAVKMCREMDVP 300
           LLDMKFLKTTEAGEQ DV+EWV EYIREKAPELLNLIT SEVFDSS GGA KMCREMDVP
Sbjct: 241 LLDMKFLKTTEAGEQTDVTEWVGEYIREKAPELLNLITYSEVFDSSGGGAAKMCREMDVP 300

Query: 301 FLGKVPLDPQLCKAAEEGRSCFGDKKCGVSASALKKIIEKLIQNQGFSTMSIDAKA 357
           FLGKVPLDPQLCKAAEEGRSCFGD+KCGVSASALKKIIEKLIQNQGFSTMSIDAKA
Sbjct: 301 FLGKVPLDPQLCKAAEEGRSCFGDQKCGVSASALKKIIEKLIQNQGFSTMSIDAKA 356

BLAST of HG10011296 vs. NCBI nr
Match: XP_008442190.1 (PREDICTED: cytosolic Fe-S cluster assembly factor NBP35 [Cucumis melo] >XP_008442191.1 PREDICTED: cytosolic Fe-S cluster assembly factor NBP35 [Cucumis melo] >XP_008442192.1 PREDICTED: cytosolic Fe-S cluster assembly factor NBP35 [Cucumis melo])

HSP 1 Score: 694.9 bits (1792), Expect = 3.6e-196
Identity = 342/356 (96.07%), Postives = 349/356 (98.03%), Query Frame = 0

Query: 1   MENSEIPENANENCPGPQSESAGKSDACQGCPNQSICATAPKGPDPDLVSIAERMATVKH 60
           MENSEIPENANENCPGP+SESAGKSDACQGCPNQSICATAPKGPDPDL+SIAERMATVKH
Sbjct: 1   MENSEIPENANENCPGPESESAGKSDACQGCPNQSICATAPKGPDPDLISIAERMATVKH 60

Query: 61  KILILSGKGGVGKSTFSTQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLG 120
           KIL+LSGKGGVGKSTFS QLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLG
Sbjct: 61  KILVLSGKGGVGKSTFSAQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLG 120

Query: 121 WSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGT 180
           WSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGT
Sbjct: 121 WSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGT 180

Query: 181 SDEHISIVQCLEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSSLCQP 240
           SDEHISIVQCL+ATGIDGAIIVTTPQQVSLIDVRKEVSFCK VGV+VLGVVENMSSLCQP
Sbjct: 181 SDEHISIVQCLKATGIDGAIIVTTPQQVSLIDVRKEVSFCKTVGVEVLGVVENMSSLCQP 240

Query: 241 LLDMKFLKTTEAGEQADVSEWVAEYIREKAPELLNLITCSEVFDSSRGGAVKMCREMDVP 300
           LLDMKFLKTTEAGEQ DV+EWV EYIREKAPELLNLIT SEVFDSS GGA KMCREMDVP
Sbjct: 241 LLDMKFLKTTEAGEQTDVTEWVGEYIREKAPELLNLITYSEVFDSSGGGAAKMCREMDVP 300

Query: 301 FLGKVPLDPQLCKAAEEGRSCFGDKKCGVSASALKKIIEKLIQNQGFSTMSIDAKA 357
           FLGKVPLDPQLCKAAEEGRSCFGD+KCGVSASALKKIIEKLIQNQGFSTMSIDAKA
Sbjct: 301 FLGKVPLDPQLCKAAEEGRSCFGDQKCGVSASALKKIIEKLIQNQGFSTMSIDAKA 356

BLAST of HG10011296 vs. NCBI nr
Match: XP_022989679.1 (cytosolic Fe-S cluster assembly factor NBP35 [Cucurbita maxima])

HSP 1 Score: 671.0 bits (1730), Expect = 5.6e-189
Identity = 329/356 (92.42%), Postives = 340/356 (95.51%), Query Frame = 0

Query: 1   MENSEIPENANENCPGPQSESAGKSDACQGCPNQSICATAPKGPDPDLVSIAERMATVKH 60
           MEN EIPE+ANENCPGPQSESAGKSDACQGCPNQSICATAPKGPDPDLVSIAERMATVKH
Sbjct: 1   MENGEIPEDANENCPGPQSESAGKSDACQGCPNQSICATAPKGPDPDLVSIAERMATVKH 60

Query: 61  KILILSGKGGVGKSTFSTQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLG 120
           KIL+LSGKGGVGKSTFS QL+FALAAMDFQVGL+DIDICGPSIPKMLGLEGQDVHQSNLG
Sbjct: 61  KILVLSGKGGVGKSTFSAQLSFALAAMDFQVGLMDIDICGPSIPKMLGLEGQDVHQSNLG 120

Query: 121 WSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGT 180
           WSPVYIESNLGVMSIGFMLPNPD+AVIWRGPRKNGLIKQFLKDVYWGELDFL+VDAPPGT
Sbjct: 121 WSPVYIESNLGVMSIGFMLPNPDDAVIWRGPRKNGLIKQFLKDVYWGELDFLIVDAPPGT 180

Query: 181 SDEHISIVQCLEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSSLCQP 240
           SDEHISIVQCLEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMS L QP
Sbjct: 181 SDEHISIVQCLEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSGLTQP 240

Query: 241 LLDMKFLKTTEAGEQADVSEWVAEYIREKAPELLNLITCSEVFDSSRGGAVKMCREMDVP 300
           LLD+KFLK TE  EQ DV+EW AEYIREKAPELLNLITCSEVFDSS GGA KMCREM VP
Sbjct: 241 LLDLKFLKATETEEQVDVTEWAAEYIREKAPELLNLITCSEVFDSSGGGAAKMCREMGVP 300

Query: 301 FLGKVPLDPQLCKAAEEGRSCFGDKKCGVSASALKKIIEKLIQNQGFSTMSIDAKA 357
           FLGKVPLDPQLCKAAEEGRSCFGD+KC VSA ALKKIIE LI++QGFSTMSID KA
Sbjct: 301 FLGKVPLDPQLCKAAEEGRSCFGDQKCRVSAPALKKIIENLIESQGFSTMSIDDKA 356

BLAST of HG10011296 vs. ExPASy Swiss-Prot
Match: Q8H1Q2 (Cytosolic Fe-S cluster assembly factor NBP35 OS=Arabidopsis thaliana OX=3702 GN=NBP35 PE=1 SV=1)

HSP 1 Score: 568.5 bits (1464), Expect = 5.2e-161
Identity = 275/343 (80.17%), Postives = 307/343 (89.50%), Query Frame = 0

Query: 1   MENSEIPENANENCPGPQSESAGKSDACQGCPNQSICATAPKGPDPDLVSIAERMATVKH 60
           MEN +IPE+ANE+CPGPQSESAGKSD+C GCPNQ  CATAPKGPDPDLV+IAERM+TVKH
Sbjct: 1   MENGDIPEDANEHCPGPQSESAGKSDSCAGCPNQEACATAPKGPDPDLVAIAERMSTVKH 60

Query: 61  KILILSGKGGVGKSTFSTQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLG 120
           KIL+LSGKGGVGKSTFS QL+FALA MD QVGL+DIDICGPSIPKMLGLEGQ++HQSNLG
Sbjct: 61  KILVLSGKGGVGKSTFSAQLSFALAGMDHQVGLMDIDICGPSIPKMLGLEGQEIHQSNLG 120

Query: 121 WSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGT 180
           WSPVY+E NLGVMSIGFMLPN DEAVIWRGPRKNGLIKQFLKDVYWGE+D+LVVDAPPGT
Sbjct: 121 WSPVYVEDNLGVMSIGFMLPNSDEAVIWRGPRKNGLIKQFLKDVYWGEIDYLVVDAPPGT 180

Query: 181 SDEHISIVQCLEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSSLCQP 240
           SDEHISIVQ L  TGIDGAIIVTTPQ+VSLIDVRKEVSFCKKVGV VLGVVENMS L QP
Sbjct: 181 SDEHISIVQYLLPTGIDGAIIVTTPQEVSLIDVRKEVSFCKKVGVPVLGVVENMSGLSQP 240

Query: 241 LLDMKFLK-TTEAGEQADVSEWVAEYIREKAPELLNLITCSEVFDSSRGGAVKMCREMDV 300
           L D+KF+K  TE G   +V+E V   +R+ APELL+++ CSEVFDSS GGA +MCREM V
Sbjct: 241 LKDVKFMKLATETGSSINVTEDVIACLRKNAPELLDIVACSEVFDSSGGGAERMCREMGV 300

Query: 301 PFLGKVPLDPQLCKAAEEGRSCFGDKKCGVSASALKKIIEKLI 343
           PFLGKVP+DPQLCKAAE+G+SCF D KC +SA ALK II+K++
Sbjct: 301 PFLGKVPMDPQLCKAAEQGKSCFEDNKCLISAPALKSIIQKVV 343

BLAST of HG10011296 vs. ExPASy Swiss-Prot
Match: Q8T2F3 (Cytosolic Fe-S cluster assembly factor NUBP1 homolog OS=Dictyostelium discoideum OX=44689 GN=nubp1 PE=3 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 9.9e-104
Identity = 179/310 (57.74%), Postives = 232/310 (74.84%), Query Frame = 0

Query: 12  ENCPGPQSESAGKSDACQGCPNQSICATAPKGPDPDLVSIAERMATVKHKILILSGKGGV 71
           ENCPG QSE +GKS AC GCPNQ ICATAPKGPDPD++ I ERM TVK+KIL+LSGKGGV
Sbjct: 11  ENCPGTQSEMSGKSAACAGCPNQQICATAPKGPDPDIIEIEERMKTVKNKILVLSGKGGV 70

Query: 72  GKSTFSTQLAFALAAMD-FQVGLLDIDICGPSIPKMLGLEGQDVHQSNLGWSPVYIESNL 131
           GKSTFS+QL+FAL+  +  +VGLLDIDICGPSIPK++GLEG+++H S  GW PVY++ NL
Sbjct: 71  GKSTFSSQLSFALSMDEKVEVGLLDIDICGPSIPKIMGLEGENIHISGQGWDPVYVQDNL 130

Query: 132 GVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGTSDEHISIVQC 191
            VMS+GF+L   ++AVIWRGP+KNG+IKQFLKDVYW +LD+LV+D PPGTSDEH+SIVQ 
Sbjct: 131 AVMSVGFLLEKEEDAVIWRGPKKNGIIKQFLKDVYWNDLDYLVIDTPPGTSDEHLSIVQY 190

Query: 192 LEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSSLCQPLLDMKFLKTT 251
           L+ + + GA+IVT+PQ V+LIDVRKE++FCKKVGV ++GVVENMS    P  + +     
Sbjct: 191 LKTSNLSGAVIVTSPQDVALIDVRKEINFCKKVGVPIIGVVENMSGFVCPKCNKE----- 250

Query: 252 EAGEQADVSEWVAEYIREKAPELLNLITCSEVFDSSRGGAVKMCREMDVPFLGKVPLDPQ 311
                                        S++F  + GGA KM ++M+VPFLG++P+DP 
Sbjct: 251 -----------------------------SQIFIPTTGGAEKMSQDMNVPFLGRIPIDPL 286

Query: 312 LCKAAEEGRS 321
           + ++ +EG+S
Sbjct: 311 IARSCDEGKS 286

BLAST of HG10011296 vs. ExPASy Swiss-Prot
Match: A7RUD5 (Cytosolic Fe-S cluster assembly factor NUBP1 homolog OS=Nematostella vectensis OX=45351 GN=v1g236650 PE=3 SV=1)

HSP 1 Score: 377.9 bits (969), Expect = 1.3e-103
Identity = 189/342 (55.26%), Postives = 242/342 (70.76%), Query Frame = 0

Query: 4   SEIPENANENCPGPQSESAGKSDACQGCPNQSICATA-PKGPDPDLVSIAERMATVKHKI 63
           +++P++A E+CPG +S+ +GK  ACQGCPNQ ICA++ P  PDPDL  I ER+++VKHKI
Sbjct: 2   ADVPKDAPEHCPGTESDKSGKVSACQGCPNQQICASSKPAPPDPDLGKIKERLSSVKHKI 61

Query: 64  LILSGKGGVGKSTFSTQLAFALAA-MDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLGW 123
           L+LSGKGGVGKSTF+  LA  LAA  D QV +LDIDICGPSIP + GL+G+ VHQS  GW
Sbjct: 62  LVLSGKGGVGKSTFTAHLAHGLAADEDRQVAVLDIDICGPSIPTVFGLQGEQVHQSGSGW 121

Query: 124 SPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGTS 183
           SPVY+E NLGVMS+GF+L  P +AVIWRGP+KNGLIKQFL+DV WG+ DFLVVD PPGTS
Sbjct: 122 SPVYVEDNLGVMSVGFLLAKPTDAVIWRGPKKNGLIKQFLRDVDWGDADFLVVDTPPGTS 181

Query: 184 DEHISIVQCLEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSSLCQPL 243
           DEH+SI+Q L  T +DGA++VTTPQ+VSL+DVRKE+SFCKKV + V+GVVENMS    P 
Sbjct: 182 DEHLSIIQYLNQTEVDGAVVVTTPQEVSLLDVRKEISFCKKVRLPVIGVVENMSVFVCP- 241

Query: 244 LDMKFLKTTEAGEQADVSEWVAEYIREKAPELLNLITCSEVFDSSRGGAVKMCREMDVPF 303
                                            N    S++F  + GGA KM  EM VPF
Sbjct: 242 ---------------------------------NCKKESQIFPPTTGGAEKMAVEMKVPF 301

Query: 304 LGKVPLDPQLCKAAEEGRSCFGDKKCGVSASALKKIIEKLIQ 344
           LG++PLDP++ +A +EG+S   +     + S+ K IIEK+++
Sbjct: 302 LGRIPLDPRIGRACDEGKSFLSEIPDSPATSSYKDIIEKIVK 309

BLAST of HG10011296 vs. ExPASy Swiss-Prot
Match: Q6P298 (Cytosolic Fe-S cluster assembly factor nubp1 OS=Danio rerio OX=7955 GN=nubp1 PE=2 SV=2)

HSP 1 Score: 376.7 bits (966), Expect = 2.9e-103
Identity = 185/340 (54.41%), Postives = 243/340 (71.47%), Query Frame = 0

Query: 4   SEIPENANENCPGPQSESAGKSDACQGCPNQSICAT-APKGPDPDLVSIAERMATVKHKI 63
           +++P +A E+CPG  S+ AGKS ACQGCPNQSICA+ A K PDP +  I ++M +VKHKI
Sbjct: 2   ADVPNDAPEHCPGTSSDQAGKSSACQGCPNQSICASGATKAPDPAIEEIKQKMTSVKHKI 61

Query: 64  LILSGKGGVGKSTFSTQLAFALAA-MDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLGW 123
           L+LSGKGGVGKSTFS  L+ ALA+    +V LLD+DICGPSIPK++GLEG+ VHQS  GW
Sbjct: 62  LVLSGKGGVGKSTFSAHLSHALASDSSKEVALLDVDICGPSIPKIMGLEGEQVHQSGSGW 121

Query: 124 SPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGTS 183
           SPVY+E NL VMSIGF+L +PD+AVIWRGP+KNG+IKQFL+DV WGE+D+L+VD PPGTS
Sbjct: 122 SPVYVEDNLAVMSIGFLLSSPDDAVIWRGPKKNGMIKQFLRDVDWGEVDYLIVDTPPGTS 181

Query: 184 DEHISIVQCLEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSSLCQPL 243
           DEH+SIVQ L   GIDGA+I+TTPQ+VSL DVRKE+ FCKKV + +LGV+ENMS    P 
Sbjct: 182 DEHLSIVQYLSGAGIDGAVIITTPQEVSLQDVRKEIRFCKKVNLPILGVIENMSGFVCPK 241

Query: 244 LDMKFLKTTEAGEQADVSEWVAEYIREKAPELLNLITCSEVFDSSRGGAVKMCREMDVPF 303
                 K T                             S++F  + GGA +MC E+++P 
Sbjct: 242 -----CKNT-----------------------------SQIFPPTTGGAQRMCEELNLPL 301

Query: 304 LGKVPLDPQLCKAAEEGRSCFGDKKCGVSASALKKIIEKL 342
           LG++PLDP++ K+ +EG+S   +     +A+A + I++K+
Sbjct: 302 LGRIPLDPRIGKSCDEGKSFLTEVPDSPAAAAYQSIVQKI 307

BLAST of HG10011296 vs. ExPASy Swiss-Prot
Match: Q3KQF0 (Cytosolic Fe-S cluster assembly factor nubp1-A OS=Xenopus laevis OX=8355 GN=nubp1-A PE=2 SV=1)

HSP 1 Score: 372.1 bits (954), Expect = 7.1e-102
Identity = 184/340 (54.12%), Postives = 239/340 (70.29%), Query Frame = 0

Query: 4   SEIPENANENCPGPQSESAGKSDACQGCPNQSICAT-APKGPDPDLVSIAERMATVKHKI 63
           ++IP+NA ++CPG  S  AGKS ACQGCPNQSICA+ A  GPDP +  I E+M+ VKHKI
Sbjct: 2   ADIPDNAPQHCPGTDSTEAGKSSACQGCPNQSICASGAAAGPDPAIEEIKEKMSLVKHKI 61

Query: 64  LILSGKGGVGKSTFSTQLAFALAAMD-FQVGLLDIDICGPSIPKMLGLEGQDVHQSNLGW 123
           L+LSGKGGVGKSTFS  LA  LA  +  +V LLD+DICGPSIPKM+GLEG+ VHQS  GW
Sbjct: 62  LVLSGKGGVGKSTFSAHLAHGLAQDEGKEVALLDVDICGPSIPKMMGLEGEQVHQSGSGW 121

Query: 124 SPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGTS 183
           SPVY+E NL VMS+GF+L +PD+AVIWRGP+KNG+IKQFL+DV WG++D+L+VD PPGTS
Sbjct: 122 SPVYVEDNLAVMSVGFLLSSPDDAVIWRGPKKNGMIKQFLRDVDWGDVDYLIVDTPPGTS 181

Query: 184 DEHISIVQCLEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSSLCQPL 243
           DEH+S+VQ L A GIDGA+I+TTPQ+VSL DVRKE++FC+KV + ++GVVENMS    P 
Sbjct: 182 DEHLSVVQYLSAAGIDGAVIITTPQEVSLQDVRKEINFCRKVKLPIIGVVENMSGFICPK 241

Query: 244 LDMKFLKTTEAGEQADVSEWVAEYIREKAPELLNLITCSEVFDSSRGGAVKMCREMDVPF 303
              +                                  S++F  + GGA KMC ++ V  
Sbjct: 242 CKNE----------------------------------SQIFPPTTGGAEKMCTDLSVSL 301

Query: 304 LGKVPLDPQLCKAAEEGRSCFGDKKCGVSASALKKIIEKL 342
           LGKVPLDP + K+ + G+S F +     +  + +KII+++
Sbjct: 302 LGKVPLDPNIGKSCDTGKSFFTEIPDSPATLSYRKIIQRI 307

BLAST of HG10011296 vs. ExPASy TrEMBL
Match: A0A1S3B5W7 (Cytosolic Fe-S cluster assembly factor NBP35 OS=Cucumis melo OX=3656 GN=LOC103486120 PE=3 SV=1)

HSP 1 Score: 694.9 bits (1792), Expect = 1.8e-196
Identity = 342/356 (96.07%), Postives = 349/356 (98.03%), Query Frame = 0

Query: 1   MENSEIPENANENCPGPQSESAGKSDACQGCPNQSICATAPKGPDPDLVSIAERMATVKH 60
           MENSEIPENANENCPGP+SESAGKSDACQGCPNQSICATAPKGPDPDL+SIAERMATVKH
Sbjct: 1   MENSEIPENANENCPGPESESAGKSDACQGCPNQSICATAPKGPDPDLISIAERMATVKH 60

Query: 61  KILILSGKGGVGKSTFSTQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLG 120
           KIL+LSGKGGVGKSTFS QLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLG
Sbjct: 61  KILVLSGKGGVGKSTFSAQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLG 120

Query: 121 WSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGT 180
           WSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGT
Sbjct: 121 WSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGT 180

Query: 181 SDEHISIVQCLEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSSLCQP 240
           SDEHISIVQCL+ATGIDGAIIVTTPQQVSLIDVRKEVSFCK VGV+VLGVVENMSSLCQP
Sbjct: 181 SDEHISIVQCLKATGIDGAIIVTTPQQVSLIDVRKEVSFCKTVGVEVLGVVENMSSLCQP 240

Query: 241 LLDMKFLKTTEAGEQADVSEWVAEYIREKAPELLNLITCSEVFDSSRGGAVKMCREMDVP 300
           LLDMKFLKTTEAGEQ DV+EWV EYIREKAPELLNLIT SEVFDSS GGA KMCREMDVP
Sbjct: 241 LLDMKFLKTTEAGEQTDVTEWVGEYIREKAPELLNLITYSEVFDSSGGGAAKMCREMDVP 300

Query: 301 FLGKVPLDPQLCKAAEEGRSCFGDKKCGVSASALKKIIEKLIQNQGFSTMSIDAKA 357
           FLGKVPLDPQLCKAAEEGRSCFGD+KCGVSASALKKIIEKLIQNQGFSTMSIDAKA
Sbjct: 301 FLGKVPLDPQLCKAAEEGRSCFGDQKCGVSASALKKIIEKLIQNQGFSTMSIDAKA 356

BLAST of HG10011296 vs. ExPASy TrEMBL
Match: A0A6J1JQ09 (Cytosolic Fe-S cluster assembly factor NBP35 OS=Cucurbita maxima OX=3661 GN=LOC111486691 PE=3 SV=1)

HSP 1 Score: 671.0 bits (1730), Expect = 2.7e-189
Identity = 329/356 (92.42%), Postives = 340/356 (95.51%), Query Frame = 0

Query: 1   MENSEIPENANENCPGPQSESAGKSDACQGCPNQSICATAPKGPDPDLVSIAERMATVKH 60
           MEN EIPE+ANENCPGPQSESAGKSDACQGCPNQSICATAPKGPDPDLVSIAERMATVKH
Sbjct: 1   MENGEIPEDANENCPGPQSESAGKSDACQGCPNQSICATAPKGPDPDLVSIAERMATVKH 60

Query: 61  KILILSGKGGVGKSTFSTQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLG 120
           KIL+LSGKGGVGKSTFS QL+FALAAMDFQVGL+DIDICGPSIPKMLGLEGQDVHQSNLG
Sbjct: 61  KILVLSGKGGVGKSTFSAQLSFALAAMDFQVGLMDIDICGPSIPKMLGLEGQDVHQSNLG 120

Query: 121 WSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGT 180
           WSPVYIESNLGVMSIGFMLPNPD+AVIWRGPRKNGLIKQFLKDVYWGELDFL+VDAPPGT
Sbjct: 121 WSPVYIESNLGVMSIGFMLPNPDDAVIWRGPRKNGLIKQFLKDVYWGELDFLIVDAPPGT 180

Query: 181 SDEHISIVQCLEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSSLCQP 240
           SDEHISIVQCLEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMS L QP
Sbjct: 181 SDEHISIVQCLEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSGLTQP 240

Query: 241 LLDMKFLKTTEAGEQADVSEWVAEYIREKAPELLNLITCSEVFDSSRGGAVKMCREMDVP 300
           LLD+KFLK TE  EQ DV+EW AEYIREKAPELLNLITCSEVFDSS GGA KMCREM VP
Sbjct: 241 LLDLKFLKATETEEQVDVTEWAAEYIREKAPELLNLITCSEVFDSSGGGAAKMCREMGVP 300

Query: 301 FLGKVPLDPQLCKAAEEGRSCFGDKKCGVSASALKKIIEKLIQNQGFSTMSIDAKA 357
           FLGKVPLDPQLCKAAEEGRSCFGD+KC VSA ALKKIIE LI++QGFSTMSID KA
Sbjct: 301 FLGKVPLDPQLCKAAEEGRSCFGDQKCRVSAPALKKIIENLIESQGFSTMSIDDKA 356

BLAST of HG10011296 vs. ExPASy TrEMBL
Match: A0A6J1ERE1 (Cytosolic Fe-S cluster assembly factor NBP35 OS=Cucurbita moschata OX=3662 GN=LOC111435180 PE=3 SV=1)

HSP 1 Score: 669.8 bits (1727), Expect = 6.1e-189
Identity = 327/356 (91.85%), Postives = 341/356 (95.79%), Query Frame = 0

Query: 1   MENSEIPENANENCPGPQSESAGKSDACQGCPNQSICATAPKGPDPDLVSIAERMATVKH 60
           MEN EIPE+ANENCPGPQSESAG+SDACQGCPNQSICATAPKGPDPDLVSIAERMATVKH
Sbjct: 1   MENGEIPEDANENCPGPQSESAGRSDACQGCPNQSICATAPKGPDPDLVSIAERMATVKH 60

Query: 61  KILILSGKGGVGKSTFSTQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLG 120
           KIL+LSGKGGVGKSTFS QL+FALAAMDFQVGL+DIDICGPSIPKMLGLEGQDVHQSNLG
Sbjct: 61  KILVLSGKGGVGKSTFSAQLSFALAAMDFQVGLMDIDICGPSIPKMLGLEGQDVHQSNLG 120

Query: 121 WSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGT 180
           WSPVYIESNLGVMSIGFMLPNPD+AVIWRGPRKNGLIKQFLKDVYWGELDFL+VDAPPGT
Sbjct: 121 WSPVYIESNLGVMSIGFMLPNPDDAVIWRGPRKNGLIKQFLKDVYWGELDFLIVDAPPGT 180

Query: 181 SDEHISIVQCLEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSSLCQP 240
           SDEHISIVQCLEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMS L QP
Sbjct: 181 SDEHISIVQCLEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSGLTQP 240

Query: 241 LLDMKFLKTTEAGEQADVSEWVAEYIREKAPELLNLITCSEVFDSSRGGAVKMCREMDVP 300
           LLD+KFLKTTE  EQ DV+EW AEYIREKAPELLNLITCSEVFDSS GGA KMCR+M VP
Sbjct: 241 LLDLKFLKTTETEEQVDVTEWAAEYIREKAPELLNLITCSEVFDSSGGGAAKMCRDMGVP 300

Query: 301 FLGKVPLDPQLCKAAEEGRSCFGDKKCGVSASALKKIIEKLIQNQGFSTMSIDAKA 357
           FLGKVPLDPQLCKAAEEGRSCFGD+KC VSA ALKKIIE L+++QGFSTMSID KA
Sbjct: 301 FLGKVPLDPQLCKAAEEGRSCFGDQKCRVSAPALKKIIENLLESQGFSTMSIDDKA 356

BLAST of HG10011296 vs. ExPASy TrEMBL
Match: A0A6J1DIY7 (Cytosolic Fe-S cluster assembly factor NBP35 OS=Momordica charantia OX=3673 GN=LOC111021512 PE=3 SV=1)

HSP 1 Score: 663.7 bits (1711), Expect = 4.4e-187
Identity = 321/351 (91.45%), Postives = 338/351 (96.30%), Query Frame = 0

Query: 1   MENSEIPENANENCPGPQSESAGKSDACQGCPNQSICATAPKGPDPDLVSIAERMATVKH 60
           M+N EIP+NANENCPGPQSESAGKSDACQGCPNQ ICATAPKGPDPDLV+IAERMATVKH
Sbjct: 1   MDNGEIPDNANENCPGPQSESAGKSDACQGCPNQEICATAPKGPDPDLVAIAERMATVKH 60

Query: 61  KILILSGKGGVGKSTFSTQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLG 120
           KIL+LSGKGGVGKSTFS QL+FALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLG
Sbjct: 61  KILVLSGKGGVGKSTFSAQLSFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLG 120

Query: 121 WSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGT 180
           WSPVY+ESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGT
Sbjct: 121 WSPVYVESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGT 180

Query: 181 SDEHISIVQCLEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSSLCQP 240
           SDEHISIVQCLEATGI+GAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMS LCQP
Sbjct: 181 SDEHISIVQCLEATGIEGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSGLCQP 240

Query: 241 LLDMKFLKTTEAGEQADVSEWVAEYIREKAPELLNLITCSEVFDSSRGGAVKMCREMDVP 300
           LLD+KFLKT +AGEQADV+EW AEYIREKAPELL+LITCSEVFDSS GGA KMCREM VP
Sbjct: 241 LLDLKFLKTNKAGEQADVTEWAAEYIREKAPELLDLITCSEVFDSSGGGAAKMCREMGVP 300

Query: 301 FLGKVPLDPQLCKAAEEGRSCFGDKKCGVSASALKKIIEKLIQNQGFSTMS 352
           FLGKVPLDPQLCKAAEEGRSCFGD++CGVSA ALK IIEKL++NQG S+ +
Sbjct: 301 FLGKVPLDPQLCKAAEEGRSCFGDQECGVSAPALKNIIEKLMENQGLSSFN 351

BLAST of HG10011296 vs. ExPASy TrEMBL
Match: A0A6J1DLB9 (Cytosolic Fe-S cluster assembly factor NBP35 OS=Momordica charantia OX=3673 GN=LOC111021512 PE=3 SV=1)

HSP 1 Score: 654.4 bits (1687), Expect = 2.6e-184
Identity = 320/359 (89.14%), Postives = 338/359 (94.15%), Query Frame = 0

Query: 1   MENSEIPENANE--------NCPGPQSESAGKSDACQGCPNQSICATAPKGPDPDLVSIA 60
           M+N EIP+NANE        +CPGPQSESAGKSDACQGCPNQ ICATAPKGPDPDLV+IA
Sbjct: 1   MDNGEIPDNANESNGFPSSSDCPGPQSESAGKSDACQGCPNQEICATAPKGPDPDLVAIA 60

Query: 61  ERMATVKHKILILSGKGGVGKSTFSTQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQ 120
           ERMATVKHKIL+LSGKGGVGKSTFS QL+FALAAMDFQVGLLDIDICGPSIPKMLGLEGQ
Sbjct: 61  ERMATVKHKILVLSGKGGVGKSTFSAQLSFALAAMDFQVGLLDIDICGPSIPKMLGLEGQ 120

Query: 121 DVHQSNLGWSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFL 180
           DVHQSNLGWSPVY+ESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFL
Sbjct: 121 DVHQSNLGWSPVYVESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFL 180

Query: 181 VVDAPPGTSDEHISIVQCLEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVE 240
           VVDAPPGTSDEHISIVQCLEATGI+GAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVE
Sbjct: 181 VVDAPPGTSDEHISIVQCLEATGIEGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVE 240

Query: 241 NMSSLCQPLLDMKFLKTTEAGEQADVSEWVAEYIREKAPELLNLITCSEVFDSSRGGAVK 300
           NMS LCQPLLD+KFLKT +AGEQADV+EW AEYIREKAPELL+LITCSEVFDSS GGA K
Sbjct: 241 NMSGLCQPLLDLKFLKTNKAGEQADVTEWAAEYIREKAPELLDLITCSEVFDSSGGGAAK 300

Query: 301 MCREMDVPFLGKVPLDPQLCKAAEEGRSCFGDKKCGVSASALKKIIEKLIQNQGFSTMS 352
           MCREM VPFLGKVPLDPQLCKAAEEGRSCFGD++CGVSA ALK IIEKL++NQG S+ +
Sbjct: 301 MCREMGVPFLGKVPLDPQLCKAAEEGRSCFGDQECGVSAPALKNIIEKLMENQGLSSFN 359

BLAST of HG10011296 vs. TAIR 10
Match: AT5G50960.1 (nucleotide binding protein 35 )

HSP 1 Score: 568.5 bits (1464), Expect = 3.7e-162
Identity = 275/343 (80.17%), Postives = 307/343 (89.50%), Query Frame = 0

Query: 1   MENSEIPENANENCPGPQSESAGKSDACQGCPNQSICATAPKGPDPDLVSIAERMATVKH 60
           MEN +IPE+ANE+CPGPQSESAGKSD+C GCPNQ  CATAPKGPDPDLV+IAERM+TVKH
Sbjct: 1   MENGDIPEDANEHCPGPQSESAGKSDSCAGCPNQEACATAPKGPDPDLVAIAERMSTVKH 60

Query: 61  KILILSGKGGVGKSTFSTQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLG 120
           KIL+LSGKGGVGKSTFS QL+FALA MD QVGL+DIDICGPSIPKMLGLEGQ++HQSNLG
Sbjct: 61  KILVLSGKGGVGKSTFSAQLSFALAGMDHQVGLMDIDICGPSIPKMLGLEGQEIHQSNLG 120

Query: 121 WSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGT 180
           WSPVY+E NLGVMSIGFMLPN DEAVIWRGPRKNGLIKQFLKDVYWGE+D+LVVDAPPGT
Sbjct: 121 WSPVYVEDNLGVMSIGFMLPNSDEAVIWRGPRKNGLIKQFLKDVYWGEIDYLVVDAPPGT 180

Query: 181 SDEHISIVQCLEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSSLCQP 240
           SDEHISIVQ L  TGIDGAIIVTTPQ+VSLIDVRKEVSFCKKVGV VLGVVENMS L QP
Sbjct: 181 SDEHISIVQYLLPTGIDGAIIVTTPQEVSLIDVRKEVSFCKKVGVPVLGVVENMSGLSQP 240

Query: 241 LLDMKFLK-TTEAGEQADVSEWVAEYIREKAPELLNLITCSEVFDSSRGGAVKMCREMDV 300
           L D+KF+K  TE G   +V+E V   +R+ APELL+++ CSEVFDSS GGA +MCREM V
Sbjct: 241 LKDVKFMKLATETGSSINVTEDVIACLRKNAPELLDIVACSEVFDSSGGGAERMCREMGV 300

Query: 301 PFLGKVPLDPQLCKAAEEGRSCFGDKKCGVSASALKKIIEKLI 343
           PFLGKVP+DPQLCKAAE+G+SCF D KC +SA ALK II+K++
Sbjct: 301 PFLGKVPMDPQLCKAAEQGKSCFEDNKCLISAPALKSIIQKVV 343

BLAST of HG10011296 vs. TAIR 10
Match: AT4G19540.1 (IND1(iron-sulfur protein required for NADH dehydrogenase)-like )

HSP 1 Score: 143.7 bits (361), Expect = 2.9e-34
Identity = 84/190 (44.21%), Postives = 114/190 (60.00%), Query Frame = 0

Query: 54  RMATVKHKILILSGKGGVGKSTFSTQLAFALA-AMDFQVGLLDIDICGPSIPKMLGLEGQ 113
           R+  VK  I + SGKGGVGKS+ +  LA ALA   + ++GLLD D+ GPS+P M+ +  +
Sbjct: 38  RLHGVKDIIAVASGKGGVGKSSTAVNLAVALANKCELKIGLLDADVYGPSVPIMMNINQK 97

Query: 114 DVHQSNLGWSPVYIESNLGV--MSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELD 173
                ++   PV    N GV  MS+G +L   D  ++WRGP     + +  K V WG+LD
Sbjct: 98  PQVNQDMKMIPV---ENYGVKCMSMG-LLVEKDAPLVWRGPMVMSALAKMTKGVDWGDLD 157

Query: 174 FLVVDAPPGTSDEHISIVQCLEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGV 233
            LVVD PPGT D  ISI Q L+   + GA+IV+TPQ V+L D  + +S   KV V +LG+
Sbjct: 158 ILVVDMPPGTGDAQISISQNLK---LSGAVIVSTPQDVALADANRGISMFDKVRVPILGL 217

Query: 234 VENMSSLCQP 241
           VENMS    P
Sbjct: 218 VENMSCFVCP 220

BLAST of HG10011296 vs. TAIR 10
Match: AT3G24430.1 (ATP binding )

HSP 1 Score: 129.4 bits (324), Expect = 5.7e-30
Identity = 71/180 (39.44%), Postives = 104/180 (57.78%), Query Frame = 0

Query: 55  MATVKHKILILSGKGGVGKSTFSTQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDV 114
           ++ + + I + S KGGVGKST +  LA+ LA M  +VG+ D D+ GPS+P M+  E + +
Sbjct: 172 LSRISNIIAVSSCKGGVGKSTVAVNLAYTLAGMGARVGIFDADVYGPSLPTMVNPESR-I 231

Query: 115 HQSNLGWSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVV 174
            + N     +     +GV  + F       A++ RGP  +G+I Q L    WGELD+LV+
Sbjct: 232 LEMNPEKKTIIPTEYMGVKLVSFGFAGQGRAIM-RGPMVSGVINQLLTTTEWGELDYLVI 291

Query: 175 DAPPGTSDEHISIVQCLEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENM 234
           D PPGT D  +++ Q    T    A+IVTTPQ+++ IDV K V    K+ V  + VVENM
Sbjct: 292 DMPPGTGDIQLTLCQVAPLT---AAVIVTTPQKLAFIDVAKGVRMFSKLKVPCVAVVENM 346

BLAST of HG10011296 vs. TAIR 10
Match: AT5G24020.1 (septum site-determining protein (MIND) )

HSP 1 Score: 54.7 bits (130), Expect = 1.8e-07
Identity = 46/182 (25.27%), Postives = 82/182 (45.05%), Query Frame = 0

Query: 62  ILILSGKGGVGKSTFSTQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQ------DVH 121
           ++I SGKGGVGK+T +  +  +LA   F V  +D D+   ++  +LGLE +      +V 
Sbjct: 61  VVITSGKGGVGKTTTTANVGLSLARYGFSVVAIDADLGLRNLDLLLGLENRVNYTCVEVI 120

Query: 122 QSNLGWSPVYIE----SNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDF 181
             +       +     SN  ++ I    P     + + G     L+   LK    G  DF
Sbjct: 121 NGDCRLDQALVRDKRWSNFELLCIS--KPRSKLPMGFGGKALEWLV-DALKTRPEGSPDF 180

Query: 182 LVVDAPPGTSDEHISIVQCLEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVV 234
           +++D P G     I+ +     T  + A++VTTP   +L D  +     +  G++ + ++
Sbjct: 181 IIIDCPAGIDAGFITAI-----TPANEAVLVTTPDITALRDADRVTGLLECDGIRDIKMI 234

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038883029.14.3e-19796.35cytosolic Fe-S cluster assembly factor NBP35 [Benincasa hispida][more]
KAE8649747.11.3e-19696.35hypothetical protein Csa_012684 [Cucumis sativus][more]
XP_004144673.11.3e-19696.35cytosolic Fe-S cluster assembly factor NBP35 [Cucumis sativus] >XP_011653919.1 c... [more]
XP_008442190.13.6e-19696.07PREDICTED: cytosolic Fe-S cluster assembly factor NBP35 [Cucumis melo] >XP_00844... [more]
XP_022989679.15.6e-18992.42cytosolic Fe-S cluster assembly factor NBP35 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q8H1Q25.2e-16180.17Cytosolic Fe-S cluster assembly factor NBP35 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q8T2F39.9e-10457.74Cytosolic Fe-S cluster assembly factor NUBP1 homolog OS=Dictyostelium discoideum... [more]
A7RUD51.3e-10355.26Cytosolic Fe-S cluster assembly factor NUBP1 homolog OS=Nematostella vectensis O... [more]
Q6P2982.9e-10354.41Cytosolic Fe-S cluster assembly factor nubp1 OS=Danio rerio OX=7955 GN=nubp1 PE=... [more]
Q3KQF07.1e-10254.12Cytosolic Fe-S cluster assembly factor nubp1-A OS=Xenopus laevis OX=8355 GN=nubp... [more]
Match NameE-valueIdentityDescription
A0A1S3B5W71.8e-19696.07Cytosolic Fe-S cluster assembly factor NBP35 OS=Cucumis melo OX=3656 GN=LOC10348... [more]
A0A6J1JQ092.7e-18992.42Cytosolic Fe-S cluster assembly factor NBP35 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1ERE16.1e-18991.85Cytosolic Fe-S cluster assembly factor NBP35 OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1DIY74.4e-18791.45Cytosolic Fe-S cluster assembly factor NBP35 OS=Momordica charantia OX=3673 GN=L... [more]
A0A6J1DLB92.6e-18489.14Cytosolic Fe-S cluster assembly factor NBP35 OS=Momordica charantia OX=3673 GN=L... [more]
Match NameE-valueIdentityDescription
AT5G50960.13.7e-16280.17nucleotide binding protein 35 [more]
AT4G19540.12.9e-3444.21IND1(iron-sulfur protein required for NADH dehydrogenase)-like [more]
AT3G24430.15.7e-3039.44ATP binding [more]
AT5G24020.11.8e-0725.27septum site-determining protein (MIND) [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR033756Flagellum site-determining protein YlxH/ Fe-S cluster assembling factor NBP35PFAMPF10609ParAcoord: 58..241
e-value: 6.0E-82
score: 274.8
coord: 279..341
e-value: 6.9E-7
score: 29.0
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 54..352
e-value: 2.1E-88
score: 298.4
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 45..321
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..27
NoneNo IPR availablePANTHERPTHR23264:SF36CYTOSOLIC FE-S CLUSTER ASSEMBLY FACTOR NBP35coord: 1..343
NoneNo IPR availableCDDcd02037Mrp_NBP35coord: 60..313
e-value: 3.23229E-109
score: 315.98
IPR019591Mrp/NBP35 ATP-binding proteinPANTHERPTHR23264NUCLEOTIDE-BINDING PROTEIN NBP35 YEAST -RELATEDcoord: 1..343
IPR019591Mrp/NBP35 ATP-binding proteinHAMAPMF_02040Mrp_NBP35coord: 53..342
score: 35.69923
IPR000808Mrp, conserved sitePROSITEPS01215MRPcoord: 166..182
IPR028601Cytosolic Fe-S cluster assembly factor NUBP1/Nbp35HAMAPMF_03038NUBP1coord: 7..346
score: 48.56847

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10011296.1HG10011296.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016226 iron-sulfur cluster assembly
cellular_component GO:0005829 cytosol
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0051536 iron-sulfur cluster binding