HG10011023 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10011023
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionRING-type E3 ubiquitin transferase
LocationChr01: 1711198 .. 1713945 (-)
RNA-Seq ExpressionHG10011023
SyntenyHG10011023
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTTGGATAGTATTGAGTGTGTATCATCTTCTGATGGTCTCGATGAAGATGAGATTCATCTTCATCACACTCTTCATCCTTATTCCCATTCTCATCATCATCCAGAGTTGTCAGCGACGAAGCCTCGTAATGGGAGCAATAATAGCGGTATAGCAGTCCCCACTGCGACTGCACCCGCCACTAGCGTCCATGAATTGCTTGAGTGCCCTGTCTGTACCAATTCTATGTACCCTCCCATCCATCAGGTTTGTCTTACGGATTGAAGGTTAATTTTGATAAATGTTGAAGGTTGCCCTAGTTACTTACAGAATGGGGAGGGGGCTGATTTGATTTTAGATTTCTAGGGTGGGGGAAGGGTCTTTTTTTATGAGATAATTTCCTTTGATTTATCTTTTCCCTGCTGGGTTTTTGTTTTTATATAAGTTTGATCATTCAGTGTTTGCTGGAAGTTTGGGGACGTGAATTGTTTTGGATGGATGTGTGAAATGGGAACTTGTTATTGAGTGCTAAAAGTGTGAGATGCAAAGTCTAGAACTAGATTATATTGAAATATAGCAAATCTTTGGTTTGATGTATGTTGTATCATATTGTAACCATAAAGCATTAGCCATCATATCTCTTTTACTTCTTAAGGTTTCTAACACTAGGCTATATAATCAAGAGATTATTACTTGTCCTATTTTTGCTTGAAAGCCTTTGTTTTAGCATAAGATGCTGTTTGTTTTAAACTACTCTGTAAGGAGGTTTTTTGGGTTTTGTTATAAACATGATTTTAACTGGCTGATGAAACTTTAGTTTCCTATTGCTTTCTTAGAATACCATTTCCCGCCATTGCAGTTATCTTTATCTTTCTTCCTGTATCATTGGCAGCATTCAGTCTATTTGTACAGATTTGGTTTAGGGGTCTAGAAATGTATTGAATCGATTTTTTTTAATGAATAGATCCGATCGTAACTTGGAATATGGAATTCAAAGGATCAAATAGGAAATGATACTCTGAATCATAGAACTATAATGAAATATACGATCAACCAACATTTATCAAATTTGAAAAAGGGTCCGAAGAAATGGTTCGAGGAGTTGGTACCATCAGGATGATGATAGCTGTTAAGATTTAAGAAGATGCTTGTTTTATTCCAATATTATCTTAAAACATTTAATACTTTTGATAAGATGTATATTTGGTTGTTGATATATATCTAATGATTGTATGTAAGTGAGGGTTAACTGTATGACCGACTAAAATGGTGCAGTGCCACAATGGACATACCTTATGTTCTACATGTAAAACACGGGTTCATAATCGTTGCCCTACTTGTAGACAAGAGCTTGGAGATATTAGGTGCTTAGCACTGGAGAAGGTGGCAGAGTCACTTGAATTGCCTTGCAAGTATTATTCATTGGGTTGCACTGAGATATTCCCATACTACAGTAAACTTAAGCATGAGGCTTTGTGCAACTACAGACCCTATAGTTGCCCATATGCTGGGTCGGAATGCGCTGCTGTTGGGGACATTCCTTTCCTTGTTGCCCATCTGAGGGATGATCATAAGGTGGATATGCACACAGGCTGCACATTTAACCATCGCTATGTTAAATCCAATCCAAGGGAAGTGGAGAATGCCACTTGGATGCTCACTGTAAGTCTGATGTATTTGACCTTAATTCTTATGTGATTTTCTTTCTTGTTGGTAGAACTTGTTTTATCAGACATGAATTACACATCTTACATATGATATAAATACAAATTCATGTGAGCATATGTCACGTGTGCATTTGTATCTATGAAGACTAGGATTACAGACCCAATTAAGCCACCAGGGAGATTAGAATAAGATCCTACTGGTCGTCAACAACAGATGTATGATGAAAAAAATCATTGATATTAGATGGCCTGCTACAAGTTTTAAGTTTTATAAACCATTGAAGATGCTTCTTAAGTTTTTCTGATGGCATGAAATGGTTATTTCTGAAAGTCAGTGACATGTTTGGTAATCAATCATTTTCTTTTATTTTATATAACTCGTGACCTTAACTGATTAACTATTTGCCTTTTTCCAGTGGCAAATGGATGTGATGCAATGAAATAAAGTTTGGTTGAAATATCAAGGGATCAAGAGAACCCCTTTTATTTAATATTTTCAATTTCCTTTTCTGCAAGTGAAGGACTTGTGCTTTCACGACTTGAAATTTTTTCTTAAAGTTTATTGTCTTTTCCGTTCCATTGATAGATTGGTCACTGGACGCTGAACTACTACCTATAAATGGCCTGTTTTGTGTATAATCAAATGACTTCACGCTGAGAGGTTCTGCTGGACATTCATTGGGTTTACTCCAAATTTTAGTTTGATCCTGTTTCTTGTTTGCTCTGTTACATTTTGCAGGTGTTCCATTGTTTTGGTCAATATTTCTGCCTTCACTTTGAAGCCTTTCAGCTTGGCATGGCTCCAGTCTATATGGCTTTCCTTCGTTTCATGGGTGATGAGAATGAGGCCCGAACATACAGCTACAGTCTTGAGGTTGGTGGATACGGGCGAAAATTAATATGGGAGGGCACCCCGCGAAGTATCAGAGATAGTCATCGAAAAGTGAGGGACAGCCATGATGGGCTAATCATTCAAAGAAATATGGCTCTTTTCTTCTCTGGTGGAGACAGGAAGGAACTGAAACTAAGAGTTACAGGAAGGATATGGAAGGAACAAAACCCAGATGCTGGAGTCTGCATACCGAACCTGTGTAGCTAA

mRNA sequence

ATGGATTTGGATAGTATTGAGTGTGTATCATCTTCTGATGGTCTCGATGAAGATGAGATTCATCTTCATCACACTCTTCATCCTTATTCCCATTCTCATCATCATCCAGAGTTGTCAGCGACGAAGCCTCGTAATGGGAGCAATAATAGCGGTATAGCAGTCCCCACTGCGACTGCACCCGCCACTAGCGTCCATGAATTGCTTGAGTGCCCTGTCTGTACCAATTCTATGTACCCTCCCATCCATCAGTGCCACAATGGACATACCTTATGTTCTACATGTAAAACACGGGTTCATAATCGTTGCCCTACTTGTAGACAAGAGCTTGGAGATATTAGGTGCTTAGCACTGGAGAAGGTGGCAGAGTCACTTGAATTGCCTTGCAAGTATTATTCATTGGGTTGCACTGAGATATTCCCATACTACAGTAAACTTAAGCATGAGGCTTTGTGCAACTACAGACCCTATAGTTGCCCATATGCTGGGTCGGAATGCGCTGCTGTTGGGGACATTCCTTTCCTTGTTGCCCATCTGAGGGATGATCATAAGGTGGATATGCACACAGGCTGCACATTTAACCATCGCTATGTTAAATCCAATCCAAGGGAAGTGGAGAATGCCACTTGGATGCTCACTGTGTTCCATTGTTTTGGTCAATATTTCTGCCTTCACTTTGAAGCCTTTCAGCTTGGCATGGCTCCAGTCTATATGGCTTTCCTTCGTTTCATGGGTGATGAGAATGAGGCCCGAACATACAGCTACAGTCTTGAGGTTGGTGGATACGGGCGAAAATTAATATGGGAGGGCACCCCGCGAAGTATCAGAGATAGTCATCGAAAAGTGAGGGACAGCCATGATGGGCTAATCATTCAAAGAAATATGGCTCTTTTCTTCTCTGGTGGAGACAGGAAGGAACTGAAACTAAGAGTTACAGGAAGGATATGGAAGGAACAAAACCCAGATGCTGGAGTCTGCATACCGAACCTGTGTAGCTAA

Coding sequence (CDS)

ATGGATTTGGATAGTATTGAGTGTGTATCATCTTCTGATGGTCTCGATGAAGATGAGATTCATCTTCATCACACTCTTCATCCTTATTCCCATTCTCATCATCATCCAGAGTTGTCAGCGACGAAGCCTCGTAATGGGAGCAATAATAGCGGTATAGCAGTCCCCACTGCGACTGCACCCGCCACTAGCGTCCATGAATTGCTTGAGTGCCCTGTCTGTACCAATTCTATGTACCCTCCCATCCATCAGTGCCACAATGGACATACCTTATGTTCTACATGTAAAACACGGGTTCATAATCGTTGCCCTACTTGTAGACAAGAGCTTGGAGATATTAGGTGCTTAGCACTGGAGAAGGTGGCAGAGTCACTTGAATTGCCTTGCAAGTATTATTCATTGGGTTGCACTGAGATATTCCCATACTACAGTAAACTTAAGCATGAGGCTTTGTGCAACTACAGACCCTATAGTTGCCCATATGCTGGGTCGGAATGCGCTGCTGTTGGGGACATTCCTTTCCTTGTTGCCCATCTGAGGGATGATCATAAGGTGGATATGCACACAGGCTGCACATTTAACCATCGCTATGTTAAATCCAATCCAAGGGAAGTGGAGAATGCCACTTGGATGCTCACTGTGTTCCATTGTTTTGGTCAATATTTCTGCCTTCACTTTGAAGCCTTTCAGCTTGGCATGGCTCCAGTCTATATGGCTTTCCTTCGTTTCATGGGTGATGAGAATGAGGCCCGAACATACAGCTACAGTCTTGAGGTTGGTGGATACGGGCGAAAATTAATATGGGAGGGCACCCCGCGAAGTATCAGAGATAGTCATCGAAAAGTGAGGGACAGCCATGATGGGCTAATCATTCAAAGAAATATGGCTCTTTTCTTCTCTGGTGGAGACAGGAAGGAACTGAAACTAAGAGTTACAGGAAGGATATGGAAGGAACAAAACCCAGATGCTGGAGTCTGCATACCGAACCTGTGTAGCTAA

Protein sequence

MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSATKPRNGSNNSGIAVPTATAPATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKVAESLELPCKYYSLGCTEIFPYYSKLKHEALCNYRPYSCPYAGSECAAVGDIPFLVAHLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQNPDAGVCIPNLCS
Homology
BLAST of HG10011023 vs. NCBI nr
Match: XP_008466823.1 (PREDICTED: E3 ubiquitin-protein ligase SINAT5-like [Cucumis melo] >XP_011651325.1 E3 ubiquitin-protein ligase SINAT5 [Cucumis sativus] >XP_031738434.1 E3 ubiquitin-protein ligase SINAT5 [Cucumis sativus] >KGN57671.1 hypothetical protein Csa_009721 [Cucumis sativus])

HSP 1 Score: 705.3 bits (1819), Expect = 2.5e-199
Identity = 328/331 (99.09%), Postives = 330/331 (99.70%), Query Frame = 0

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSATKPRNGSNNSGIAVPTATAP 60
           MDLDSIECVSSSDGLDEDEIHLHHTLHPYS SHHHPELSATKPRNGSNNSGIAVPTATAP
Sbjct: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSLSHHHPELSATKPRNGSNNSGIAVPTATAP 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCTEIFPYYSKLKHEALCNYRPYSCPYAGSECAAVGDIPFLVAHLRD 180
           AESLELPCKYYSLGCTEIFPYYSKLKHEALCNYRPYSCPYAGSEC+AVGDIPFLVAHLRD
Sbjct: 121 AESLELPCKYYSLGCTEIFPYYSKLKHEALCNYRPYSCPYAGSECSAVGDIPFLVAHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDEN+ARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG
Sbjct: 241 RFMGDENDARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 332
           GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS
Sbjct: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 331

BLAST of HG10011023 vs. NCBI nr
Match: XP_038891628.1 (E3 ubiquitin-protein ligase SINAT5 [Benincasa hispida])

HSP 1 Score: 704.9 bits (1818), Expect = 3.3e-199
Identity = 326/331 (98.49%), Postives = 329/331 (99.40%), Query Frame = 0

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSATKPRNGSNNSGIAVPTATAP 60
           MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSATKPRNG+NNSGI VPTATAP
Sbjct: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSATKPRNGNNNSGITVPTATAP 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCTEIFPYYSKLKHEALCNYRPYSCPYAGSECAAVGDIPFLVAHLRD 180
           AESLELPCKYYSLGCTEIFPYYSKLKHEALCNYRPYSCPYAGSEC+AVGDIPFLVAHLRD
Sbjct: 121 AESLELPCKYYSLGCTEIFPYYSKLKHEALCNYRPYSCPYAGSECSAVGDIPFLVAHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDEN+ARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG
Sbjct: 241 RFMGDENDARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 332
           GDRKELKLRVTGRIWKEQNPDAGVC PNLCS
Sbjct: 301 GDRKELKLRVTGRIWKEQNPDAGVCTPNLCS 331

BLAST of HG10011023 vs. NCBI nr
Match: XP_022141336.1 (E3 ubiquitin-protein ligase SINAT5-like [Momordica charantia])

HSP 1 Score: 697.6 bits (1799), Expect = 5.2e-197
Identity = 323/331 (97.58%), Postives = 327/331 (98.79%), Query Frame = 0

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSATKPRNGSNNSGIAVPTATAP 60
           MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSA+KPRNGSNNS I  PTATAP
Sbjct: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSASKPRNGSNNSAITGPTATAP 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCTEIFPYYSKLKHEALCNYRPYSCPYAGSECAAVGDIPFLVAHLRD 180
           AESLELPCKYYSLGC EIFPYYSKLKHEALCN+RPY+CPYAGSEC+AVGDIPFLVAHLRD
Sbjct: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVAHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG
Sbjct: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 332
           GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS
Sbjct: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 331

BLAST of HG10011023 vs. NCBI nr
Match: XP_022984623.1 (E3 ubiquitin-protein ligase SINAT5 isoform X1 [Cucurbita maxima])

HSP 1 Score: 695.3 bits (1793), Expect = 2.6e-196
Identity = 322/331 (97.28%), Postives = 327/331 (98.79%), Query Frame = 0

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSATKPRNGSNNSGIAVPTATAP 60
           MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELS+TKPRNGSNNS I  PTATAP
Sbjct: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSSTKPRNGSNNSAITGPTATAP 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCTEIFPYYSKLKHEALCNYRPYSCPYAGSECAAVGDIPFLVAHLRD 180
           AESLELPCKYYSLGC EIFPYYSKLKHEALCN+RPY+CPYAGSEC+AVGDIPFLVAHLRD
Sbjct: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVAHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVF+CFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFNCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG
Sbjct: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 332
           GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS
Sbjct: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 331

BLAST of HG10011023 vs. NCBI nr
Match: XP_022922771.1 (E3 ubiquitin-protein ligase SINAT5 isoform X1 [Cucurbita moschata] >XP_023553000.1 E3 ubiquitin-protein ligase SINAT5 isoform X1 [Cucurbita pepo subsp. pepo] >KAG7014703.1 E3 ubiquitin-protein ligase SINAT4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 693.7 bits (1789), Expect = 7.5e-196
Identity = 321/331 (96.98%), Postives = 327/331 (98.79%), Query Frame = 0

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSATKPRNGSNNSGIAVPTATAP 60
           MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELS+TKPRNGSNNS I  PTATAP
Sbjct: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSSTKPRNGSNNSAITGPTATAP 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCTEIFPYYSKLKHEALCNYRPYSCPYAGSECAAVGDIPFLVAHLRD 180
           AESLELPCKYYSLGC EIFPYYSKLKHEALCN+RPY+CPYAGSEC+AVGDIPFLVAHLRD
Sbjct: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVAHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMH+GCTFNHRYVKSNPREVENATWMLTVF+CFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHSGCTFNHRYVKSNPREVENATWMLTVFNCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG
Sbjct: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 332
           GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS
Sbjct: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 331

BLAST of HG10011023 vs. ExPASy Swiss-Prot
Match: Q84JL3 (E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana OX=3702 GN=SINAT3 PE=1 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 5.3e-160
Identity = 266/332 (80.12%), Postives = 280/332 (84.34%), Query Frame = 0

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSATKPRNGSNNSGIAVPTATAP 60
           MDLDS++C S+ D  D++EIH     +     HHH         N + N   A       
Sbjct: 1   MDLDSMDCTSTMDVTDDEEIHQDRHSYASVSKHHH-------TNNNTTNVNAAASGLLPT 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
            TSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCK RVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  TTSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKARVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCTEIFPYYSKLKHEALCNYRPYSCPYAGSECAAVGDIPFLVAHLRD 180
           AESLELPCK+ SLGC EIFPYYSKLKHE +CN+RPYSCPYAGSEC+  GDIPFLVAHLRD
Sbjct: 121 AESLELPCKHMSLGCPEIFPYYSKLKHETVCNFRPYSCPYAGSECSVTGDIPFLVAHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMH+GCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHSGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDE EAR Y+YSLEVGGYGRKLIWEGTPRS+RDSHRKVRDSHDGLIIQRNMALFFSG
Sbjct: 241 RFMGDETEARNYNYSLEVGGYGRKLIWEGTPRSVRDSHRKVRDSHDGLIIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKEQNPD---AGVCIPNL 330
           GDRKELKLRVTGRIWKEQ       G CIPNL
Sbjct: 301 GDRKELKLRVTGRIWKEQQQSGEGGGACIPNL 325

BLAST of HG10011023 vs. ExPASy Swiss-Prot
Match: Q9STN8 (E3 ubiquitin-protein ligase SINAT4 OS=Arabidopsis thaliana OX=3702 GN=SINAT4 PE=1 SV=1)

HSP 1 Score: 558.5 bits (1438), Expect = 5.0e-158
Identity = 266/336 (79.17%), Postives = 289/336 (86.01%), Query Frame = 0

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSATKPRNGSNNSGIAV----PT 60
           M+ DS+ECVSS+           + +H   + H   + S+TK   G+  + +      PT
Sbjct: 1   METDSMECVSSTG----------NEIHQNGNGHQSYQFSSTKTHGGAAAAAVVTNIVGPT 60

Query: 61  ATAPATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLA 120
           ATAPATSV+ELLECPVCT SMYPPIHQCHNGHTLCSTCK RVHNRCPTCRQELGDIRCLA
Sbjct: 61  ATAPATSVYELLECPVCTYSMYPPIHQCHNGHTLCSTCKVRVHNRCPTCRQELGDIRCLA 120

Query: 121 LEKVAESLELPCKYYSLGCTEIFPYYSKLKHEALCNYRPYSCPYAGSECAAVGDIPFLVA 180
           LEKVAESLELPCK+Y+LGC EIFPYYSKLKHE+LCN+RPYSCPYAGSEC  VGDIPFLVA
Sbjct: 121 LEKVAESLELPCKFYNLGCPEIFPYYSKLKHESLCNFRPYSCPYAGSECGIVGDIPFLVA 180

Query: 181 HLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVY 240
           HLRDDHKVDMH G TFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGM PVY
Sbjct: 181 HLRDDHKVDMHAGSTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMGPVY 240

Query: 241 MAFLRFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMAL 300
           MAFLRFMGDE +AR+YSYSLEVGG GRKL WEGTPRSIRDSHRKVRDS+DGLIIQRNMAL
Sbjct: 241 MAFLRFMGDEEDARSYSYSLEVGGSGRKLTWEGTPRSIRDSHRKVRDSNDGLIIQRNMAL 300

Query: 301 FFSGGDRKELKLRVTGRIWKEQ-NPDAGVCIPNLCS 332
           FFSGGDRKELKLRVTG+IWKEQ +PD+G+ IPNL S
Sbjct: 301 FFSGGDRKELKLRVTGKIWKEQHSPDSGLSIPNLSS 326

BLAST of HG10011023 vs. ExPASy Swiss-Prot
Match: Q8S3N1 (E3 ubiquitin-protein ligase SINAT5 OS=Arabidopsis thaliana OX=3702 GN=SINAT5 PE=1 SV=2)

HSP 1 Score: 555.1 bits (1429), Expect = 5.5e-157
Identity = 264/332 (79.52%), Postives = 286/332 (86.14%), Query Frame = 0

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSATKPRNGSNNSGIAVPTATAP 60
           M+ DSI+ V     +D+DEIH  H            + S+TK + G+           +P
Sbjct: 1   METDSIDSV-----IDDDEIHQKH------------QFSSTKSQGGAT-------VVISP 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           ATSV+ELLECPVCTNSMYPPIHQCHNGHTLCSTCK+RVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  ATSVYELLECPVCTNSMYPPIHQCHNGHTLCSTCKSRVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCTEIFPYYSKLKHEALCNYRPYSCPYAGSECAAVGDIPFLVAHLRD 180
           AESLELPCKYY+LGC  IFPYYSKLKHE+ CN+RPYSCPYAGSECAAVGDI FLVAHLRD
Sbjct: 121 AESLELPCKYYNLGCLGIFPYYSKLKHESQCNFRPYSCPYAGSECAAVGDITFLVAHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVF CFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFQCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDE++AR Y+YSLEVGG GRK  WEGTPRS+RDSHRKVRDSHDGLIIQRNMALFFSG
Sbjct: 241 RFMGDEDDARNYTYSLEVGGSGRKQTWEGTPRSVRDSHRKVRDSHDGLIIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKE-QNPDAGVCIPNLCS 332
           GD+KELKLRVTGRIWKE QNPD+GVCI ++CS
Sbjct: 301 GDKKELKLRVTGRIWKEQQNPDSGVCITSMCS 308

BLAST of HG10011023 vs. ExPASy Swiss-Prot
Match: Q10L91 (E3 ubiquitin-protein ligase DIS1 OS=Oryza sativa subsp. japonica OX=39947 GN=DIS1 PE=1 SV=1)

HSP 1 Score: 480.3 bits (1235), Expect = 1.7e-134
Identity = 220/273 (80.59%), Postives = 239/273 (87.55%), Query Frame = 0

Query: 50  SGIAVPTATAP----ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTC 109
           +G  VP +  P    ++SV ELLECPVC ++MYPPIHQC NGHTLCS CK RVHNRCPTC
Sbjct: 29  AGDPVPHSPKPNVVVSSSVRELLECPVCLSAMYPPIHQCSNGHTLCSGCKPRVHNRCPTC 88

Query: 110 RQELGDIRCLALEKVAESLELPCKYYSLGCTEIFPYYSKLKHEALCNYRPYSCPYAGSEC 169
           R ELG+IRCLALEKVA SLELPCKY + GC  I+PYY KLKHE+ C YRPYSCPYAGSEC
Sbjct: 89  RHELGNIRCLALEKVAASLELPCKYQNFGCVGIYPYYCKLKHESQCQYRPYSCPYAGSEC 148

Query: 170 AAVGDIPFLVAHLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHF 229
              GDIP+LV HL+DDHKVDMH GCTFNHRYVKSNP EVENATWMLTVF CFGQYFCLHF
Sbjct: 149 TVAGDIPYLVNHLKDDHKVDMHNGCTFNHRYVKSNPHEVENATWMLTVFSCFGQYFCLHF 208

Query: 230 EAFQLGMAPVYMAFLRFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSH 289
           EAFQLGMAPVY+AFLRFMGD+ EA+ YSYSLEVGG GRK+IW+G PRSIRDSHRKVRDS+
Sbjct: 209 EAFQLGMAPVYIAFLRFMGDDLEAKNYSYSLEVGGTGRKMIWQGVPRSIRDSHRKVRDSY 268

Query: 290 DGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ 319
           DGLIIQRNMALFFSGG+RKELKLRVTGRIWKEQ
Sbjct: 269 DGLIIQRNMALFFSGGERKELKLRVTGRIWKEQ 301

BLAST of HG10011023 vs. ExPASy Swiss-Prot
Match: Q9M2P4 (E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana OX=3702 GN=SINAT2 PE=1 SV=1)

HSP 1 Score: 468.8 bits (1205), Expect = 5.2e-131
Identity = 210/255 (82.35%), Postives = 230/255 (90.20%), Query Frame = 0

Query: 64  VHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKVAES 123
           V+ELLECPVCTN MYPPIHQC NGHTLCS CK RV N CPTCR ELG+IRCLALEKVAES
Sbjct: 54  VYELLECPVCTNLMYPPIHQCPNGHTLCSNCKLRVQNTCPTCRYELGNIRCLALEKVAES 113

Query: 124 LELPCKYYSLGCTEIFPYYSKLKHEALCNYRPYSCPYAGSECAAVGDIPFLVAHLRDDHK 183
           LE+PC+Y +LGC +IFPYYSKLKHE  C +RPY+CPYAGSEC+  GDIP LV HL+DDHK
Sbjct: 114 LEVPCRYQNLGCHDIFPYYSKLKHEQHCRFRPYTCPYAGSECSVTGDIPTLVVHLKDDHK 173

Query: 184 VDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFM 243
           VDMH GCTFNHRYVKSNP EVENATWMLTVF+CFG+ FCLHFEAFQLGMAPVYMAFLRFM
Sbjct: 174 VDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFM 233

Query: 244 GDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSGGDR 303
           GDENEA+ +SYSLEVG +GRKL W+G PRSIRDSHRKVRDS DGLII RN+AL+FSGGDR
Sbjct: 234 GDENEAKKFSYSLEVGAHGRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGGDR 293

Query: 304 KELKLRVTGRIWKEQ 319
           +ELKLRVTGRIWKE+
Sbjct: 294 QELKLRVTGRIWKEE 308

BLAST of HG10011023 vs. ExPASy TrEMBL
Match: A0A0A0LC94 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G239840 PE=3 SV=1)

HSP 1 Score: 705.3 bits (1819), Expect = 1.2e-199
Identity = 328/331 (99.09%), Postives = 330/331 (99.70%), Query Frame = 0

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSATKPRNGSNNSGIAVPTATAP 60
           MDLDSIECVSSSDGLDEDEIHLHHTLHPYS SHHHPELSATKPRNGSNNSGIAVPTATAP
Sbjct: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSLSHHHPELSATKPRNGSNNSGIAVPTATAP 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCTEIFPYYSKLKHEALCNYRPYSCPYAGSECAAVGDIPFLVAHLRD 180
           AESLELPCKYYSLGCTEIFPYYSKLKHEALCNYRPYSCPYAGSEC+AVGDIPFLVAHLRD
Sbjct: 121 AESLELPCKYYSLGCTEIFPYYSKLKHEALCNYRPYSCPYAGSECSAVGDIPFLVAHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDEN+ARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG
Sbjct: 241 RFMGDENDARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 332
           GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS
Sbjct: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 331

BLAST of HG10011023 vs. ExPASy TrEMBL
Match: A0A1S3CS31 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC107990291 PE=3 SV=1)

HSP 1 Score: 705.3 bits (1819), Expect = 1.2e-199
Identity = 328/331 (99.09%), Postives = 330/331 (99.70%), Query Frame = 0

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSATKPRNGSNNSGIAVPTATAP 60
           MDLDSIECVSSSDGLDEDEIHLHHTLHPYS SHHHPELSATKPRNGSNNSGIAVPTATAP
Sbjct: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSLSHHHPELSATKPRNGSNNSGIAVPTATAP 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCTEIFPYYSKLKHEALCNYRPYSCPYAGSECAAVGDIPFLVAHLRD 180
           AESLELPCKYYSLGCTEIFPYYSKLKHEALCNYRPYSCPYAGSEC+AVGDIPFLVAHLRD
Sbjct: 121 AESLELPCKYYSLGCTEIFPYYSKLKHEALCNYRPYSCPYAGSECSAVGDIPFLVAHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDEN+ARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG
Sbjct: 241 RFMGDENDARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 332
           GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS
Sbjct: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 331

BLAST of HG10011023 vs. ExPASy TrEMBL
Match: A0A6J1CIV8 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111011754 PE=3 SV=1)

HSP 1 Score: 697.6 bits (1799), Expect = 2.5e-197
Identity = 323/331 (97.58%), Postives = 327/331 (98.79%), Query Frame = 0

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSATKPRNGSNNSGIAVPTATAP 60
           MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSA+KPRNGSNNS I  PTATAP
Sbjct: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSASKPRNGSNNSAITGPTATAP 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCTEIFPYYSKLKHEALCNYRPYSCPYAGSECAAVGDIPFLVAHLRD 180
           AESLELPCKYYSLGC EIFPYYSKLKHEALCN+RPY+CPYAGSEC+AVGDIPFLVAHLRD
Sbjct: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVAHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG
Sbjct: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 332
           GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS
Sbjct: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 331

BLAST of HG10011023 vs. ExPASy TrEMBL
Match: A0A6J1JB27 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111482855 PE=3 SV=1)

HSP 1 Score: 695.3 bits (1793), Expect = 1.3e-196
Identity = 322/331 (97.28%), Postives = 327/331 (98.79%), Query Frame = 0

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSATKPRNGSNNSGIAVPTATAP 60
           MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELS+TKPRNGSNNS I  PTATAP
Sbjct: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSSTKPRNGSNNSAITGPTATAP 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCTEIFPYYSKLKHEALCNYRPYSCPYAGSECAAVGDIPFLVAHLRD 180
           AESLELPCKYYSLGC EIFPYYSKLKHEALCN+RPY+CPYAGSEC+AVGDIPFLVAHLRD
Sbjct: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVAHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVF+CFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFNCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG
Sbjct: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 332
           GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS
Sbjct: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 331

BLAST of HG10011023 vs. ExPASy TrEMBL
Match: A0A6J1E513 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111430668 PE=3 SV=1)

HSP 1 Score: 693.7 bits (1789), Expect = 3.7e-196
Identity = 321/331 (96.98%), Postives = 327/331 (98.79%), Query Frame = 0

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSATKPRNGSNNSGIAVPTATAP 60
           MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELS+TKPRNGSNNS I  PTATAP
Sbjct: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSSTKPRNGSNNSAITGPTATAP 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCTEIFPYYSKLKHEALCNYRPYSCPYAGSECAAVGDIPFLVAHLRD 180
           AESLELPCKYYSLGC EIFPYYSKLKHEALCN+RPY+CPYAGSEC+AVGDIPFLVAHLRD
Sbjct: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVAHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMH+GCTFNHRYVKSNPREVENATWMLTVF+CFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHSGCTFNHRYVKSNPREVENATWMLTVFNCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG
Sbjct: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 332
           GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS
Sbjct: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 331

BLAST of HG10011023 vs. TAIR 10
Match: AT3G61790.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 565.1 bits (1455), Expect = 3.8e-161
Identity = 266/332 (80.12%), Postives = 280/332 (84.34%), Query Frame = 0

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSATKPRNGSNNSGIAVPTATAP 60
           MDLDS++C S+ D  D++EIH     +     HHH         N + N   A       
Sbjct: 1   MDLDSMDCTSTMDVTDDEEIHQDRHSYASVSKHHH-------TNNNTTNVNAAASGLLPT 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
            TSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCK RVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  TTSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKARVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCTEIFPYYSKLKHEALCNYRPYSCPYAGSECAAVGDIPFLVAHLRD 180
           AESLELPCK+ SLGC EIFPYYSKLKHE +CN+RPYSCPYAGSEC+  GDIPFLVAHLRD
Sbjct: 121 AESLELPCKHMSLGCPEIFPYYSKLKHETVCNFRPYSCPYAGSECSVTGDIPFLVAHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMH+GCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHSGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDE EAR Y+YSLEVGGYGRKLIWEGTPRS+RDSHRKVRDSHDGLIIQRNMALFFSG
Sbjct: 241 RFMGDETEARNYNYSLEVGGYGRKLIWEGTPRSVRDSHRKVRDSHDGLIIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKEQNPD---AGVCIPNL 330
           GDRKELKLRVTGRIWKEQ       G CIPNL
Sbjct: 301 GDRKELKLRVTGRIWKEQQQSGEGGGACIPNL 325

BLAST of HG10011023 vs. TAIR 10
Match: AT4G27880.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 558.5 bits (1438), Expect = 3.5e-159
Identity = 266/336 (79.17%), Postives = 289/336 (86.01%), Query Frame = 0

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSATKPRNGSNNSGIAV----PT 60
           M+ DS+ECVSS+           + +H   + H   + S+TK   G+  + +      PT
Sbjct: 1   METDSMECVSSTG----------NEIHQNGNGHQSYQFSSTKTHGGAAAAAVVTNIVGPT 60

Query: 61  ATAPATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLA 120
           ATAPATSV+ELLECPVCT SMYPPIHQCHNGHTLCSTCK RVHNRCPTCRQELGDIRCLA
Sbjct: 61  ATAPATSVYELLECPVCTYSMYPPIHQCHNGHTLCSTCKVRVHNRCPTCRQELGDIRCLA 120

Query: 121 LEKVAESLELPCKYYSLGCTEIFPYYSKLKHEALCNYRPYSCPYAGSECAAVGDIPFLVA 180
           LEKVAESLELPCK+Y+LGC EIFPYYSKLKHE+LCN+RPYSCPYAGSEC  VGDIPFLVA
Sbjct: 121 LEKVAESLELPCKFYNLGCPEIFPYYSKLKHESLCNFRPYSCPYAGSECGIVGDIPFLVA 180

Query: 181 HLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVY 240
           HLRDDHKVDMH G TFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGM PVY
Sbjct: 181 HLRDDHKVDMHAGSTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMGPVY 240

Query: 241 MAFLRFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMAL 300
           MAFLRFMGDE +AR+YSYSLEVGG GRKL WEGTPRSIRDSHRKVRDS+DGLIIQRNMAL
Sbjct: 241 MAFLRFMGDEEDARSYSYSLEVGGSGRKLTWEGTPRSIRDSHRKVRDSNDGLIIQRNMAL 300

Query: 301 FFSGGDRKELKLRVTGRIWKEQ-NPDAGVCIPNLCS 332
           FFSGGDRKELKLRVTG+IWKEQ +PD+G+ IPNL S
Sbjct: 301 FFSGGDRKELKLRVTGKIWKEQHSPDSGLSIPNLSS 326

BLAST of HG10011023 vs. TAIR 10
Match: AT3G58040.1 (seven in absentia of Arabidopsis 2 )

HSP 1 Score: 468.8 bits (1205), Expect = 3.7e-132
Identity = 210/255 (82.35%), Postives = 230/255 (90.20%), Query Frame = 0

Query: 64  VHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKVAES 123
           V+ELLECPVCTN MYPPIHQC NGHTLCS CK RV N CPTCR ELG+IRCLALEKVAES
Sbjct: 54  VYELLECPVCTNLMYPPIHQCPNGHTLCSNCKLRVQNTCPTCRYELGNIRCLALEKVAES 113

Query: 124 LELPCKYYSLGCTEIFPYYSKLKHEALCNYRPYSCPYAGSECAAVGDIPFLVAHLRDDHK 183
           LE+PC+Y +LGC +IFPYYSKLKHE  C +RPY+CPYAGSEC+  GDIP LV HL+DDHK
Sbjct: 114 LEVPCRYQNLGCHDIFPYYSKLKHEQHCRFRPYTCPYAGSECSVTGDIPTLVVHLKDDHK 173

Query: 184 VDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFM 243
           VDMH GCTFNHRYVKSNP EVENATWMLTVF+CFG+ FCLHFEAFQLGMAPVYMAFLRFM
Sbjct: 174 VDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFM 233

Query: 244 GDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSGGDR 303
           GDENEA+ +SYSLEVG +GRKL W+G PRSIRDSHRKVRDS DGLII RN+AL+FSGGDR
Sbjct: 234 GDENEAKKFSYSLEVGAHGRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGGDR 293

Query: 304 KELKLRVTGRIWKEQ 319
           +ELKLRVTGRIWKE+
Sbjct: 294 QELKLRVTGRIWKEE 308

BLAST of HG10011023 vs. TAIR 10
Match: AT2G41980.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 461.1 bits (1185), Expect = 7.7e-130
Identity = 207/258 (80.23%), Postives = 229/258 (88.76%), Query Frame = 0

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           +  V+ELLECPVCTN MYPPIHQC NGHTLCS+CK RV N CPTCR ELG+IRCLALEKV
Sbjct: 48  SNGVYELLECPVCTNLMYPPIHQCPNGHTLCSSCKLRVQNTCPTCRYELGNIRCLALEKV 107

Query: 121 AESLELPCKYYSLGCTEIFPYYSKLKHEALCNYRPYSCPYAGSECAAVGDIPFLVAHLRD 180
           AESLE+PC+Y +LGC +IFPYYSKLKHE  C +R YSCPYAGSEC+  GDIP LV HL+D
Sbjct: 108 AESLEVPCRYQNLGCQDIFPYYSKLKHEQHCRFRSYSCPYAGSECSVTGDIPTLVDHLKD 167

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMH GCTFNHRYVKSNP EVENATWMLTVF+CFG+ FCLHFEAFQLGMAPVYMAFL
Sbjct: 168 DHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFL 227

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDENEA+ +SYSLEVG + RKL W+G PRSIRDSHRKVRDS DGLII RN+AL+FSG
Sbjct: 228 RFMGDENEAKKFSYSLEVGAHSRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSG 287

Query: 301 GDRKELKLRVTGRIWKEQ 319
            D++ELKLRVTGRIWKE+
Sbjct: 288 SDKEELKLRVTGRIWKEE 305

BLAST of HG10011023 vs. TAIR 10
Match: AT5G53360.1 (TRAF-like superfamily protein )

HSP 1 Score: 426.0 bits (1094), Expect = 2.7e-119
Identity = 195/218 (89.45%), Postives = 207/218 (94.95%), Query Frame = 0

Query: 115 LALEKVAESLELPCKYYSLGCTEIFPYYSKLKHEALCNYRPYSCPYAGSECAAVGDIPFL 174
           +ALEKVAESLELPCKYY+LGC  IFPYYSKLKHE+ CN+RPYSCPYAGSECAAVGDI FL
Sbjct: 15  IALEKVAESLELPCKYYNLGCLGIFPYYSKLKHESQCNFRPYSCPYAGSECAAVGDITFL 74

Query: 175 VAHLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAP 234
           VAHLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVF CFGQYFCLHFEAFQLGMAP
Sbjct: 75  VAHLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFQCFGQYFCLHFEAFQLGMAP 134

Query: 235 VYMAFLRFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNM 294
           VYMAFLRFMGDE++AR Y+YSLEVGG GRK  WEGTPRS+RDSHRKVRDSHDGLIIQRNM
Sbjct: 135 VYMAFLRFMGDEDDARNYTYSLEVGGSGRKQTWEGTPRSVRDSHRKVRDSHDGLIIQRNM 194

Query: 295 ALFFSGGDRKELKLRVTGRIWKE-QNPDAGVCIPNLCS 332
           ALFFSGGD+KELKLRVTGRIWKE QNPD+GVCI ++CS
Sbjct: 195 ALFFSGGDKKELKLRVTGRIWKEQQNPDSGVCITSMCS 232

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008466823.12.5e-19999.09PREDICTED: E3 ubiquitin-protein ligase SINAT5-like [Cucumis melo] >XP_011651325.... [more]
XP_038891628.13.3e-19998.49E3 ubiquitin-protein ligase SINAT5 [Benincasa hispida][more]
XP_022141336.15.2e-19797.58E3 ubiquitin-protein ligase SINAT5-like [Momordica charantia][more]
XP_022984623.12.6e-19697.28E3 ubiquitin-protein ligase SINAT5 isoform X1 [Cucurbita maxima][more]
XP_022922771.17.5e-19696.98E3 ubiquitin-protein ligase SINAT5 isoform X1 [Cucurbita moschata] >XP_023553000... [more]
Match NameE-valueIdentityDescription
Q84JL35.3e-16080.12E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana OX=3702 GN=SINAT3 PE=... [more]
Q9STN85.0e-15879.17E3 ubiquitin-protein ligase SINAT4 OS=Arabidopsis thaliana OX=3702 GN=SINAT4 PE=... [more]
Q8S3N15.5e-15779.52E3 ubiquitin-protein ligase SINAT5 OS=Arabidopsis thaliana OX=3702 GN=SINAT5 PE=... [more]
Q10L911.7e-13480.59E3 ubiquitin-protein ligase DIS1 OS=Oryza sativa subsp. japonica OX=39947 GN=DIS... [more]
Q9M2P45.2e-13182.35E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana OX=3702 GN=SINAT2 PE=... [more]
Match NameE-valueIdentityDescription
A0A0A0LC941.2e-19999.09RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G239840 PE... [more]
A0A1S3CS311.2e-19999.09RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC107990291 PE=3 ... [more]
A0A6J1CIV82.5e-19797.58RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101175... [more]
A0A6J1JB271.3e-19697.28RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111482855 P... [more]
A0A6J1E5133.7e-19696.98RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111430668... [more]
Match NameE-valueIdentityDescription
AT3G61790.13.8e-16180.12Protein with RING/U-box and TRAF-like domains [more]
AT4G27880.13.5e-15979.17Protein with RING/U-box and TRAF-like domains [more]
AT3G58040.13.7e-13282.35seven in absentia of Arabidopsis 2 [more]
AT2G41980.17.7e-13080.23Protein with RING/U-box and TRAF-like domains [more]
AT5G53360.12.7e-11989.45TRAF-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008974TRAF-likeGENE3D2.60.210.10Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2; Chain Acoord: 177..311
e-value: 5.9E-38
score: 131.6
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 31..122
e-value: 6.9E-7
score: 30.9
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 123..176
e-value: 3.1E-17
score: 64.5
IPR018121Seven-in-absentia protein, TRAF-like domainPFAMPF03145Sinacoord: 112..311
e-value: 7.5E-79
score: 264.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 28..55
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 41..55
NoneNo IPR availablePANTHERPTHR10315E3 UBIQUITIN PROTEIN LIGASE SIAHcoord: 1..323
NoneNo IPR availablePANTHERPTHR10315:SF127E3 UBIQUITIN-PROTEIN LIGASEcoord: 1..323
NoneNo IPR availableCDDcd16571RING-HC_SIAHscoord: 68..106
e-value: 1.44383E-14
score: 64.9986
NoneNo IPR availableCDDcd03829Sinacoord: 184..312
e-value: 7.43249E-68
score: 206.854
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 57..109
NoneNo IPR availableSUPERFAMILY49599TRAF domain-likecoord: 123..315
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 70..106
score: 9.464913
IPR013010Zinc finger, SIAH-typePROSITEPS51081ZF_SIAHcoord: 123..183
score: 14.125784

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10011023.1HG10011023.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007275 multicellular organism development
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0016874 ligase activity
molecular_function GO:0005515 protein binding
molecular_function GO:0061630 ubiquitin protein ligase activity
molecular_function GO:0008270 zinc ion binding