HG10009056 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10009056
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Descriptionlong chain acyl-CoA synthetase 4-like
LocationChr06: 2081269 .. 2086948 (+)
RNA-Seq ExpressionHG10009056
SyntenyHG10009056
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAGAGGAAGTACCTGGTTCAGGTGGAGGAGGCAAAGGAAGCCGCCGCAGGCCGGCCGTCGGTCGGGCCGGTCTACCGGAGCATTTTTGCTAAAGATGGGTTCCCCCCGCCGGTTCAAGGCTTGGAAACCTGCTGGGATATATTTCGGTATTATATTTCTTTTAATGGTTTTGTTGATCTGTTTGATGGATGAGAAACCCCAAGAAGAAAATTCTAAACTTTATATAGTTTTTGGAATGTAAACTCTGTTTTCTTTGTGTTTTGATGAATCTTTGTGGATCCCCCAGTAGGAAAACTGGATGGTTTTCGATCTGGTCGTGTCAAAGAAGTTCTGAACTTGGTTGTTTTTGTGGTTATAGTATGTGTAAACTGTGGTGCCACTTGGTTAAACTAAAGTTTAAGTTCTTAAACTTTTAAGTTTGTGCTTATTTCTTCTTTGAACTTTAAAAGTGTTTGTAGATTACTGAACTTTCAATTTTGTATCGAGTAAGTTCTTAAACTTCAAAATATCTATTACGTCTTTGAACTTTTAATTTTGTGTCTAGTAGGTTCTTGTCATATTCAAAAAGGTTTTGAAATAGTTGATCAATTAGATATAAAATTGAATCTTATGTATAATGAGATCCTAAACTTGGAATTTTGTGTTTAATAGATCCGTGAGTTTTTGAAAATATCAAATAGGTTGAGGACGGACAAAATCGAAAGTTTAAGAACCTATTAGTCATTTTTTGAAGTTTAGGGACCTAAGCTATAAGTTGGACACTTTTTGAAGTTTAAGGATCAAGTAGATATAGACAGACTAAAATTATTCCACGAAGTTAAGTTTTGTAGCCTTGTAAACTAAATTTTACTAAAATCATGAGCAAGACATTATCAGCTCATGGCTTTGGTAAATTTGGAGTAAGAAACTTTGTGTTGGGTCTATGAATTTGTAATTTGTAAAAAAGAAGGTTTAAGGAGCTAGAAATCTTGTATTTTGTAACCAAATATAAGCTTGACTTTACAAACTCCAGACTATACAAATCCACCTCATAAATAAAACGCTCTACGAACACTTGTGACCATAATGCTTGATGCAGAATGTCTGTGGAGAAAAATCCTGGAAACCGGATGCTTGGACGTCGGAAAATAGTTGAGGGAAATCCAGTAAGTTACTCATAAAGAAGTTGCTTTGGCATTGAACTGAATAGGGTAAAATGTTACGGTGGATTCTGTGCATCTGCAGGCTAAAGCTGGTGAATGTGAGTGGCTAACTTACAAAGAAGTCTATGAATTGGTGTTGAAAATTGGAAACGCAATGCGCGGTCTGGGTTATGGACCAGTAAGTTCTTGAAAATTTTTGTGTTTGTAAAATGCTATACAAATTTAGGAGCTAACATTTGTAGTTGTGCTGAGTTTGCAGGGAGTAAAATGTGGAATTTATGGTGCTAATTGTCCTGAGTGGATCATTAGCATGGAGGTAATTAGTTCATTATATACTTCTTCGTCAGTTACATAAATCAATGTTTTCCGTTTGTAAGCAGACGGTAGTAATGTAAGACTCTATTTGTTGACCTTTTGGCTTTTTGTTTCTTGTTTTGCAGGCTTGCAATGCTCATGGGCTTTATTGTGTTCCTTTATATGATACTTTAGGTAAAGACATGGTATAAAAGTTATTTTTTTATTAAACTTTGAGAATTATTTTAGTCCATGAACTTTACCTAAAAAAGGAATCTTTTAGTCCTTGCTGTTTCTTAATGAACTATTCACATCTTTTGTTCATTCCTTTATTGATAAGTCTTTGAAGCCACATGTAGTAGGTTAGGTGCTAGTTTCTATATTAATCTGGGCTCTTGTTAAATGATTTATAGAAGAGTGACAAGGACTAAAAACAGTTTCTATGTGAAGTTTAGGGACTGAATTAAAATGTTCAAAAGTTTAGAGATCAAAATGGAATAAATAGGAAAGTCTAAATAGGTTTCGGACATGAAAACGACAAACGACCTTCTCGGCCACGTCTCTCAAGTTTTTCCTGCTTTGTTTCCCATACCATATAAAAGTTATTTTGGATTCCAACTTTTTGTAATATGTTTCAGGTGCTGGTGCCATAGAATTCATAATATGCCATGCAGAGATTTCCATTTCTTTTGTAGAAGAGAAGAAGATCTCTGAGGTTCAACGCCATCTTATTTGCAACTAAACAAGAGCCATATAGTTTTAAGTAAAATGTCTTTGATATTAACTTTTAACTCTCTCTTATTCAGCTGTTGAAAACACTCCCCAACACAACAAAGTTTCTTAAAAGTAAGTCCTTACTCTTTTTTCATACACCTATAAAAGTTGTCTGTATCTAACAAGATTTTAATTTTGTTATGTAGCCGTTGTGAGCTTTGGTAAAGTTTCAGATAATCACAAGGAAGAGGTTAACAAGTTTGGTTTGGAAATATATTCTTGGGAAGAGTTTCTGCAAAAGGTAAGTTATATAAAAGTATATGTAGGACACTTAGCTCACAATTTTAGTGAAGTCTTTGGACTAGGGAGGCCTGGACTTGTGTATCTTGTGTTTGGATTTTGATTAAAAGATAGAAGTTTATCTAGGTCTGTGTTTGATTTGTGAGTGTACTTTGTAAAATTAACCATTTTGTCCTAAATAGGTTTTAGAAGTCTAGATTTGTGTCCGCAAATCTGTAACGTAGGATACGTGAACCTACCTACTGAAATTAGGCTCGATTTCACAACTCCAAGCTATTCAAATCTATCGCGTGAAACAAATGATGTGTGGTTTGTCTAACTCCAAAGTATGGATGTATCGAAATCACACTAAATTGAGTGACAATTTGGATCATTTAACTGTCATATGTGAACAAAATTGAATATCATACCTCTTTCTCCTCTCAGAATGTTGTTTTTACTACTGGAAACAGGGAGATAGCCAACAATATGATCTTCCAGTGAAAGGGAAAGGTGATATTTGCACCATCATGTACACCAGTGGAACAACTGGTGATCCCAAGGGAGTACTGTTATCCAATAATGCCATTATAGCACTTATAGCTGGTGTCAAACATTTGCTTACAACTGTAAAGGAACAGGTGACCTCTGTTCTTCCCATTCCCCTCTACAAATGGATAACTCAATTTTCAAGCTTGTTGATTCATGAGATGCATATTTGTTCGTGTTTCTTGTTATAGGTTCAAGAAAATGATGTTTACATATCATATCTTCCACTTGCGCACATCTTCGACCGGGTGATCGAGGAGGTGTTCATTTTGAACAGTGCCTCCATCGGATTCTGGCGTGGGGTGAGAAATCATATTTTGGTCATTCCCTCTTTTAAGGCAAAGAAATTGAAGACTCATGTCATGTCCATGAATTTTGTAGGATGTGAAATTGTTAGTCGAAGACATTGGAGAGTTGAAACCAACTATTTTCTGTGCAGTTCCTCGTGTGCTCGAAAGAATTTATGGTGGTGAGCATATGATATGTTGCTTTGGGTTTGATATTAATGGCATATGCGTTTAGATTCGAGCTAATTATTGAGCCAACATGTAAAAGATATTAAACTTATATGAACCAATCAGTTTAAGCTTTTGAATATTTTGTTTGTCAAACACGGTTTAAAATTAAACTTCTAATTATTAGAATGGTGGATAACATCGAAAAGTGTTTAATGGCATTGCTTAGGTTTGAACCAGAAGATATCGTCGGGAGGCTTCTTAAAAAAATCGTTGTTTAACTTTGCATACTCATAGTAAGTACATTTTCCCTCTCAAAATATGATACTTTCTTATCAACATTGTGCTATATTTCTCACTTCATATTTACTTTTGAATGATTTGGACAAAGTTCAGATTCAAGTTTCTGTTGTTTTTAATTCATCCATGTTGTTCTATCTCTCTTCTTTTTGTTTTTCTTTTTGTATTTATGCAGTAAACATAACCAAATGCAGAAGGGGAAAAAACATGAAGAGGCAGCCCCACTTTTTGATAAAGTTGTCTTTGACAAGGTAAAAGGGAAAAAAAGACAAACTTATACAATTATAAGGCTAGACTTCAATCAAAATCATCTTAAAAGATTTGAAAACTCATTCATCTTCTTGGAGGGATATTTTTAGGTGAAGAAAGGATTAGGAGGAAAAGTGCGAATCATTTTATCTGGAGGAGCCCCTCTTGCATCACATATCGAAACTTTCTTTCGAGTTGTATCGTGTGCTCATGTTCTACAAGGATATGGTATGTCATCCTTGTACCTCCATTTCCACAAAACAAAGCAACTGGATCCCAAATTTCATACATTTCTATTTGCAGGTTTGACAGAAACTTGTGGGGGAACATTTGTCACACTACCAAATGAATTGCCAATGTTGGGGACAGTAGGCCCTCCAGTGCCCAATGTGGATGTCTGTCTCGAATCGGTACCCGAGATGGGGTACGATTCGCATGCTAGCACGCCTCGAGGTGAAGTATGCATCAAGGGAGATACATTGTTTTCGGGGTACTACAAACGCGATGATCTTACCACAGAGGTGTTGGTTGATGGATGGTTTCATACAGGTACTACCTAGTTTTTTTGAACTTTTCGTCTCTCGAAGTTCAAAAGCATGTCGATATTGCTCAAAGGTTTGATTCTTACATTCTTTGTATCATATTAGGGGATGTTGGCGAATGGCAACCGGATGGAAGCTTGAAAATTATTGACCGTAAGAAGAACATCTTCAAACTTTCCCAAGGTGAATATGTTGCAGTGGAAAATCTAGAGCTGATTTATGGCCTTGTTACCGACATCGAAATGGTATGAATTCTAGCTCCTATTATTATTCATAACTAGAGAATTTATATTCTACAACGGCAAAAGAGTCGCAAAAAGTCAAAAAAAACGTCACCATAACTTATGACATCAAAACTATGTAGTATGAAGTTTTAGCGATACAAAGTATAAACGCCGTTTCGCTAAAAATTAACTTAGTGACGTTTTCCTAAAGAAAAAATTTGCTGAACACACACACATATATATATTGCAGTGGTTCTTTCCATTGATAATGTTTGATTGAGGATTTTTTTCAGATATGGATATATGGAAATAGCTTTGAGTCCTTCCTTGTTGCTGTTGTTAACCCTAAAAAGCAATCACTTGAGCATTGGGCAGAAGAAAATGGAATAAAAGGGGACTTTAATGCTCTTTGTGAAGACAAAAGGGCAAAAGACTATATACTTGGACAACTATCAAAGATTGCCAAAGAAAAAAAGGTTCATTTTTTCTTTCTTGTCATGATTATGAAGATGACCTTGTTGAAAGTAGCATCAATTGAAGTTATGGTTTTTGCTTTCGCAGTTAAAAGGTTACGAAAATATTAGAGCGGTTCATCTCGATCCCCTTCCGTTCGATATAGAACGTGATCTTCTTACTCCATCATTTAAGAAGAAGAGACCACAACTACTCAAATACTATCAGGTCCTTCTCTACTTCACTTGATACTATTAATTTACACTTTATAGTCTCAATGTTTTAACGACATTTATACATTTTCATTATGGTCTCTATAATCTGGCTAAAACTTATTAAGAGTTAATACTTCTGATTAGCTCTAATCGAACTATAGAGACGATTAATAAAAGATATAATATAGACTAACGACAAAATTTTGTTAACAGAAAGAGATCGATGACATGTACAAGAGTGGGAACAAGCCAGTTGCCTAA

mRNA sequence

ATGCAGAGGAAGTACCTGGTTCAGGTGGAGGAGGCAAAGGAAGCCGCCGCAGGCCGGCCGTCGGTCGGGCCGGTCTACCGGAGCATTTTTGCTAAAGATGGGTTCCCCCCGCCGGTTCAAGGCTTGGAAACCTGCTGGGATATATTTCGAATGTCTGTGGAGAAAAATCCTGGAAACCGGATGCTTGGACGTCGGAAAATAGTTGAGGGAAATCCAGCTAAAGCTGGTGAATGTGAGTGGCTAACTTACAAAGAAGTCTATGAATTGGTGTTGAAAATTGGAAACGCAATGCGCGGTCTGGGTTATGGACCAGGAGTAAAATGTGGAATTTATGGTGCTAATTGTCCTGAGTGGATCATTAGCATGGAGGCTTGCAATGCTCATGGGCTTTATTGTGTTCCTTTATATGATACTTTAGGTGCTGGTGCCATAGAATTCATAATATGCCATGCAGAGATTTCCATTTCTTTTGTAGAAGAGAAGAAGATCTCTGAGCTGTTGAAAACACTCCCCAACACAACAAAGTTTCTTAAAACCGTTGTGAGCTTTGGTAAAGTTTCAGATAATCACAAGGAAGAGGTTAACAAGTTTGGTTTGGAAATATATTCTTGGGAAGAGTTTCTGCAAAAGGGAGATAGCCAACAATATGATCTTCCAGTGAAAGGGAAAGGTGATATTTGCACCATCATGTACACCAGTGGAACAACTGGTGATCCCAAGGGAGTACTGTTATCCAATAATGCCATTATAGCACTTATAGCTGGTGTCAAACATTTGCTTACAACTGTAAAGGAACAGGTTCAAGAAAATGATGTTTACATATCATATCTTCCACTTGCGCACATCTTCGACCGGGTGATCGAGGAGGTGTTCATTTTGAACAGTGCCTCCATCGGATTCTGGCGTGGGGATGTGAAATTGTTAGTCGAAGACATTGGAGAGTTGAAACCAACTATTTTCTGTGCAGTTCCTCGTGTGCTCGAAAGAATTTATGGTGGTTTGAACCAGAAGATATCGTCGGGAGGCTTCTTAAAAAAATCGTTGTTTAACTTTGCATACTCATATAAACATAACCAAATGCAGAAGGGGAAAAAACATGAAGAGGCAGCCCCACTTTTTGATAAAGTTGTCTTTGACAAGGTGAAGAAAGGATTAGGAGGAAAAGTGCGAATCATTTTATCTGGAGGAGCCCCTCTTGCATCACATATCGAAACTTTCTTTCGAGTTGTATCGTGTGCTCATGTTCTACAAGGATATGGTTTGACAGAAACTTGTGGGGGAACATTTGTCACACTACCAAATGAATTGCCAATGTTGGGGACAGTAGGCCCTCCAGTGCCCAATGTGGATGTCTGTCTCGAATCGGTACCCGAGATGGGGTACGATTCGCATGCTAGCACGCCTCGAGGTGAAGTATGCATCAAGGGAGATACATTGTTTTCGGGGTACTACAAACGCGATGATCTTACCACAGAGGTGTTGGTTGATGGATGGTTTCATACAGGGGATGTTGGCGAATGGCAACCGGATGGAAGCTTGAAAATTATTGACCGTAAGAAGAACATCTTCAAACTTTCCCAAGGTGAATATGTTGCAGTGGAAAATCTAGAGCTGATTTATGGCCTTGTTACCGACATCGAAATGATATGGATATATGGAAATAGCTTTGAGTCCTTCCTTGTTGCTGTTGTTAACCCTAAAAAGCAATCACTTGAGCATTGGGCAGAAGAAAATGGAATAAAAGGGGACTTTAATGCTCTTTGTGAAGACAAAAGGGCAAAAGACTATATACTTGGACAACTATCAAAGATTGCCAAAGAAAAAAAGTTAAAAGGTTACGAAAATATTAGAGCGGTTCATCTCGATCCCCTTCCGTTCGATATAGAACGTGATCTTCTTACTCCATCATTTAAGAAGAAGAGACCACAACTACTCAAATACTATCAGAAAGAGATCGATGACATGTACAAGAGTGGGAACAAGCCAGTTGCCTAA

Coding sequence (CDS)

ATGCAGAGGAAGTACCTGGTTCAGGTGGAGGAGGCAAAGGAAGCCGCCGCAGGCCGGCCGTCGGTCGGGCCGGTCTACCGGAGCATTTTTGCTAAAGATGGGTTCCCCCCGCCGGTTCAAGGCTTGGAAACCTGCTGGGATATATTTCGAATGTCTGTGGAGAAAAATCCTGGAAACCGGATGCTTGGACGTCGGAAAATAGTTGAGGGAAATCCAGCTAAAGCTGGTGAATGTGAGTGGCTAACTTACAAAGAAGTCTATGAATTGGTGTTGAAAATTGGAAACGCAATGCGCGGTCTGGGTTATGGACCAGGAGTAAAATGTGGAATTTATGGTGCTAATTGTCCTGAGTGGATCATTAGCATGGAGGCTTGCAATGCTCATGGGCTTTATTGTGTTCCTTTATATGATACTTTAGGTGCTGGTGCCATAGAATTCATAATATGCCATGCAGAGATTTCCATTTCTTTTGTAGAAGAGAAGAAGATCTCTGAGCTGTTGAAAACACTCCCCAACACAACAAAGTTTCTTAAAACCGTTGTGAGCTTTGGTAAAGTTTCAGATAATCACAAGGAAGAGGTTAACAAGTTTGGTTTGGAAATATATTCTTGGGAAGAGTTTCTGCAAAAGGGAGATAGCCAACAATATGATCTTCCAGTGAAAGGGAAAGGTGATATTTGCACCATCATGTACACCAGTGGAACAACTGGTGATCCCAAGGGAGTACTGTTATCCAATAATGCCATTATAGCACTTATAGCTGGTGTCAAACATTTGCTTACAACTGTAAAGGAACAGGTTCAAGAAAATGATGTTTACATATCATATCTTCCACTTGCGCACATCTTCGACCGGGTGATCGAGGAGGTGTTCATTTTGAACAGTGCCTCCATCGGATTCTGGCGTGGGGATGTGAAATTGTTAGTCGAAGACATTGGAGAGTTGAAACCAACTATTTTCTGTGCAGTTCCTCGTGTGCTCGAAAGAATTTATGGTGGTTTGAACCAGAAGATATCGTCGGGAGGCTTCTTAAAAAAATCGTTGTTTAACTTTGCATACTCATATAAACATAACCAAATGCAGAAGGGGAAAAAACATGAAGAGGCAGCCCCACTTTTTGATAAAGTTGTCTTTGACAAGGTGAAGAAAGGATTAGGAGGAAAAGTGCGAATCATTTTATCTGGAGGAGCCCCTCTTGCATCACATATCGAAACTTTCTTTCGAGTTGTATCGTGTGCTCATGTTCTACAAGGATATGGTTTGACAGAAACTTGTGGGGGAACATTTGTCACACTACCAAATGAATTGCCAATGTTGGGGACAGTAGGCCCTCCAGTGCCCAATGTGGATGTCTGTCTCGAATCGGTACCCGAGATGGGGTACGATTCGCATGCTAGCACGCCTCGAGGTGAAGTATGCATCAAGGGAGATACATTGTTTTCGGGGTACTACAAACGCGATGATCTTACCACAGAGGTGTTGGTTGATGGATGGTTTCATACAGGGGATGTTGGCGAATGGCAACCGGATGGAAGCTTGAAAATTATTGACCGTAAGAAGAACATCTTCAAACTTTCCCAAGGTGAATATGTTGCAGTGGAAAATCTAGAGCTGATTTATGGCCTTGTTACCGACATCGAAATGATATGGATATATGGAAATAGCTTTGAGTCCTTCCTTGTTGCTGTTGTTAACCCTAAAAAGCAATCACTTGAGCATTGGGCAGAAGAAAATGGAATAAAAGGGGACTTTAATGCTCTTTGTGAAGACAAAAGGGCAAAAGACTATATACTTGGACAACTATCAAAGATTGCCAAAGAAAAAAAGTTAAAAGGTTACGAAAATATTAGAGCGGTTCATCTCGATCCCCTTCCGTTCGATATAGAACGTGATCTTCTTACTCCATCATTTAAGAAGAAGAGACCACAACTACTCAAATACTATCAGAAAGAGATCGATGACATGTACAAGAGTGGGAACAAGCCAGTTGCCTAA

Protein sequence

MQRKYLVQVEEAKEAAAGRPSVGPVYRSIFAKDGFPPPVQGLETCWDIFRMSVEKNPGNRMLGRRKIVEGNPAKAGECEWLTYKEVYELVLKIGNAMRGLGYGPGVKCGIYGANCPEWIISMEACNAHGLYCVPLYDTLGAGAIEFIICHAEISISFVEEKKISELLKTLPNTTKFLKTVVSFGKVSDNHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPKGVLLSNNAIIALIAGVKHLLTTVKEQVQENDVYISYLPLAHIFDRVIEEVFILNSASIGFWRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGFLKKSLFNFAYSYKHNQMQKGKKHEEAAPLFDKVVFDKVKKGLGGKVRIILSGGAPLASHIETFFRVVSCAHVLQGYGLTETCGGTFVTLPNELPMLGTVGPPVPNVDVCLESVPEMGYDSHASTPRGEVCIKGDTLFSGYYKRDDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIYGLVTDIEMIWIYGNSFESFLVAVVNPKKQSLEHWAEENGIKGDFNALCEDKRAKDYILGQLSKIAKEKKLKGYENIRAVHLDPLPFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGNKPVA
Homology
BLAST of HG10009056 vs. NCBI nr
Match: XP_004143868.1 (long chain acyl-CoA synthetase 4 [Cucumis sativus])

HSP 1 Score: 1312.0 bits (3394), Expect = 0.0e+00
Identity = 632/664 (95.18%), Postives = 651/664 (98.04%), Query Frame = 0

Query: 1   MQRKYLVQVEEAKEAAAGRPSVGPVYRSIFAKDGFPPPVQGLETCWDIFRMSVEKNPGNR 60
           MQRKYLVQVEEAK+A AGRPSVGPVYRSIFAKDGFPPPVQGL+TCWDIFRMSVEKNPGNR
Sbjct: 1   MQRKYLVQVEEAKDATAGRPSVGPVYRSIFAKDGFPPPVQGLDTCWDIFRMSVEKNPGNR 60

Query: 61  MLGRRKIVEGNPAKAGECEWLTYKEVYELVLKIGNAMRGLGYGPGVKCGIYGANCPEWII 120
           MLGRRK+VEGNPAKAGE EWLTYKEVYELVLKIGNAMR LGYGPG KCGIYGANCPEWII
Sbjct: 61  MLGRRKVVEGNPAKAGEYEWLTYKEVYELVLKIGNAMRSLGYGPGEKCGIYGANCPEWII 120

Query: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAEISISFVEEKKISELLKTLPNTTKFLKTV 180
           SMEACNAHGLYCVPLYDTLGAGAIEFIICHAEI+I+FVEEKKISELLKTLPNT KFLKT+
Sbjct: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAEIAIAFVEEKKISELLKTLPNTAKFLKTI 180

Query: 181 VSFGKVSDNHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPK 240
           VSFGKVSDNHKEEVNKFGLEIY+WEEFLQKGDSQQYDLPVK K DICTIMYTSGTTGDPK
Sbjct: 181 VSFGKVSDNHKEEVNKFGLEIYTWEEFLQKGDSQQYDLPVKEKSDICTIMYTSGTTGDPK 240

Query: 241 GVLLSNNAIIALIAGVKHLLTTVKEQVQENDVYISYLPLAHIFDRVIEEVFILNSASIGF 300
           GVLLSN AIIALIAGVKHLLTTVKE VQENDVYISYLPLAHIFDRVIEEVFILN ASIGF
Sbjct: 241 GVLLSNTAIIALIAGVKHLLTTVKENVQENDVYISYLPLAHIFDRVIEEVFILNGASIGF 300

Query: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGFLKKSLFNFAYSYKHNQM 360
           WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGFLK+SLFNFAYSYK+NQM
Sbjct: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGFLKRSLFNFAYSYKYNQM 360

Query: 361 QKGKKHEEAAPLFDKVVFDKVKKGLGGKVRIILSGGAPLASHIETFFRVVSCAHVLQGYG 420
           QKGK H++AAPLFDKVVFDKVKKGLGG VRIILSGGAPLA+HIETFFRVVSCAHVLQGYG
Sbjct: 361 QKGKTHQQAAPLFDKVVFDKVKKGLGGNVRIILSGGAPLAAHIETFFRVVSCAHVLQGYG 420

Query: 421 LTETCGGTFVTLPNELPMLGTVGPPVPNVDVCLESVPEMGYDSHASTPRGEVCIKGDTLF 480
           LTETCGGTFV+LP+ELPMLGTVGPPVPNVD+CLESVPEMGYDSHASTPRGEVCIKGDTLF
Sbjct: 421 LTETCGGTFVSLPDELPMLGTVGPPVPNVDICLESVPEMGYDSHASTPRGEVCIKGDTLF 480

Query: 481 SGYYKRDDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY 540
           SGYYKRDDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY
Sbjct: 481 SGYYKRDDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY 540

Query: 541 GLVTDIEMIWIYGNSFESFLVAVVNPKKQSLEHWAEENGIKGDFNALCEDKRAKDYILGQ 600
           GLV+DIEMIWIYGNSFESFLVAVVNPKKQ+LE WAEENGIKGDFNALCEDKRAKDYILGQ
Sbjct: 541 GLVSDIEMIWIYGNSFESFLVAVVNPKKQALEQWAEENGIKGDFNALCEDKRAKDYILGQ 600

Query: 601 LSKIAKEKKLKGYENIRAVHLDPLPFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGN 660
           LSKIAKE+KLKGYENIRAVHLDPLPFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGN
Sbjct: 601 LSKIAKEQKLKGYENIRAVHLDPLPFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGN 660

Query: 661 KPVA 665
           KP+A
Sbjct: 661 KPIA 664

BLAST of HG10009056 vs. NCBI nr
Match: XP_008437390.1 (PREDICTED: long chain acyl-CoA synthetase 4-like [Cucumis melo] >TYK06087.1 long chain acyl-CoA synthetase 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 1306.6 bits (3380), Expect = 0.0e+00
Identity = 631/664 (95.03%), Postives = 649/664 (97.74%), Query Frame = 0

Query: 1   MQRKYLVQVEEAKEAAAGRPSVGPVYRSIFAKDGFPPPVQGLETCWDIFRMSVEKNPGNR 60
           MQRKYLVQVEEAK+A AGRPSVGPVYRSIFAKDGFPPPVQGL+TCWDIFRMSVEKNPGNR
Sbjct: 1   MQRKYLVQVEEAKDATAGRPSVGPVYRSIFAKDGFPPPVQGLDTCWDIFRMSVEKNPGNR 60

Query: 61  MLGRRKIVEGNPAKAGECEWLTYKEVYELVLKIGNAMRGLGYGPGVKCGIYGANCPEWII 120
           MLGRRK+VEGNPAKAGE EWLTYKEVYELVLKIGNAMRGLGYGPG KCGIYGANCPEWII
Sbjct: 61  MLGRRKVVEGNPAKAGEYEWLTYKEVYELVLKIGNAMRGLGYGPGEKCGIYGANCPEWII 120

Query: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAEISISFVEEKKISELLKTLPNTTKFLKTV 180
           SMEACNAHGLYCVPLYDTLGAGAIEFII HAEISI+FVEEKKISELLKTLPNT KFLKT+
Sbjct: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIISHAEISIAFVEEKKISELLKTLPNTAKFLKTI 180

Query: 181 VSFGKVSDNHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPK 240
           VSFGKVSDNHKEEV KFGLEIY+WEEFLQKGDSQQ+DLPVK K DICTIMYTSGTTGDPK
Sbjct: 181 VSFGKVSDNHKEEVKKFGLEIYTWEEFLQKGDSQQFDLPVKEKSDICTIMYTSGTTGDPK 240

Query: 241 GVLLSNNAIIALIAGVKHLLTTVKEQVQENDVYISYLPLAHIFDRVIEEVFILNSASIGF 300
           GVLLSNNAIIALIAGVKHLLTTV E VQENDVYISYLPLAHIFDRVIEEVFILN ASIGF
Sbjct: 241 GVLLSNNAIIALIAGVKHLLTTVNENVQENDVYISYLPLAHIFDRVIEEVFILNGASIGF 300

Query: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGFLKKSLFNFAYSYKHNQM 360
           WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGFLK+SLFN AYSYK+NQM
Sbjct: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGFLKRSLFNLAYSYKYNQM 360

Query: 361 QKGKKHEEAAPLFDKVVFDKVKKGLGGKVRIILSGGAPLASHIETFFRVVSCAHVLQGYG 420
           QKGKKH++AAPLFDKVVFDKVKKGLGG VRIILSGGAPLA+HIETFFRVVSCAHVLQGYG
Sbjct: 361 QKGKKHQQAAPLFDKVVFDKVKKGLGGNVRIILSGGAPLAAHIETFFRVVSCAHVLQGYG 420

Query: 421 LTETCGGTFVTLPNELPMLGTVGPPVPNVDVCLESVPEMGYDSHASTPRGEVCIKGDTLF 480
           LTETCGGTFVTLP+ELPMLGTVGPPVPNVD+CLESVPEMGYDSHASTPRGEVCIKGDTLF
Sbjct: 421 LTETCGGTFVTLPDELPMLGTVGPPVPNVDICLESVPEMGYDSHASTPRGEVCIKGDTLF 480

Query: 481 SGYYKRDDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY 540
           SGYYKRDDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY
Sbjct: 481 SGYYKRDDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY 540

Query: 541 GLVTDIEMIWIYGNSFESFLVAVVNPKKQSLEHWAEENGIKGDFNALCEDKRAKDYILGQ 600
           GLV+DIEMIWIYGNSFESFLVAVVNPKKQ+LE WAEENGIK DFNALCEDKRAKDYILGQ
Sbjct: 541 GLVSDIEMIWIYGNSFESFLVAVVNPKKQALEQWAEENGIKEDFNALCEDKRAKDYILGQ 600

Query: 601 LSKIAKEKKLKGYENIRAVHLDPLPFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGN 660
           LSKIAKE+KLKGYENIRAVHLDPLPFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGN
Sbjct: 601 LSKIAKEQKLKGYENIRAVHLDPLPFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGN 660

Query: 661 KPVA 665
           KP+A
Sbjct: 661 KPIA 664

BLAST of HG10009056 vs. NCBI nr
Match: XP_038876249.1 (long chain acyl-CoA synthetase 4-like [Benincasa hispida])

HSP 1 Score: 1306.6 bits (3380), Expect = 0.0e+00
Identity = 634/664 (95.48%), Postives = 649/664 (97.74%), Query Frame = 0

Query: 1   MQRKYLVQVEEAKEAAAGRPSVGPVYRSIFAKDGFPPPVQGLETCWDIFRMSVEKNPGNR 60
           MQRKYLVQVEEAKEAAA RPSVGPVYRSIFAKDGFPPPVQGLETCWDIFRMSVEKNPGNR
Sbjct: 1   MQRKYLVQVEEAKEAAADRPSVGPVYRSIFAKDGFPPPVQGLETCWDIFRMSVEKNPGNR 60

Query: 61  MLGRRKIVEGNPAKAGECEWLTYKEVYELVLKIGNAMRGLGYGPGVKCGIYGANCPEWII 120
           MLGRRKIVEG+PAKAG  EWLTYKEVYELVLKIGNA+R LGYGPG KCGIYGANCPEWII
Sbjct: 61  MLGRRKIVEGDPAKAGGYEWLTYKEVYELVLKIGNAVRSLGYGPGEKCGIYGANCPEWII 120

Query: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAEISISFVEEKKISELLKTLPNTTKFLKTV 180
           SMEACNAHGLYCVPLYDTLGAGAIEFIICHAEISI+FVEEKKISELLKTLPNT KFLKT+
Sbjct: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAEISITFVEEKKISELLKTLPNTAKFLKTI 180

Query: 181 VSFGKVSDNHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPK 240
           VSFGKVSDNHKEEVNKFGLE+YSWEEFLQKGDSQQYDLPVK K DICTIMYTSGTTGDPK
Sbjct: 181 VSFGKVSDNHKEEVNKFGLEMYSWEEFLQKGDSQQYDLPVKEKSDICTIMYTSGTTGDPK 240

Query: 241 GVLLSNNAIIALIAGVKHLLTTVKEQVQENDVYISYLPLAHIFDRVIEEVFILNSASIGF 300
           GVLLSNNAIIALIAGVKHLLTTVKE+VQENDVYISYLPLAHIFDRVIEEVFILN ASIGF
Sbjct: 241 GVLLSNNAIIALIAGVKHLLTTVKEEVQENDVYISYLPLAHIFDRVIEEVFILNGASIGF 300

Query: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGFLKKSLFNFAYSYKHNQM 360
           WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGG LKKSLFNFAYSYK+ QM
Sbjct: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGLLKKSLFNFAYSYKYKQM 360

Query: 361 QKGKKHEEAAPLFDKVVFDKVKKGLGGKVRIILSGGAPLASHIETFFRVVSCAHVLQGYG 420
           QKGKKHEEAAPLFDKVVFDKVKKGLGG VRIILSGGAPLA+HIETFFRVVSCAHVLQGYG
Sbjct: 361 QKGKKHEEAAPLFDKVVFDKVKKGLGGNVRIILSGGAPLAAHIETFFRVVSCAHVLQGYG 420

Query: 421 LTETCGGTFVTLPNELPMLGTVGPPVPNVDVCLESVPEMGYDSHASTPRGEVCIKGDTLF 480
           LTETCGGTFVTLPNELPMLGTVGPPVPNVD+CLESVPEMGYDS A+TPRGEVCIKGDTLF
Sbjct: 421 LTETCGGTFVTLPNELPMLGTVGPPVPNVDICLESVPEMGYDSLANTPRGEVCIKGDTLF 480

Query: 481 SGYYKRDDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY 540
           SGYYKRDDLT EVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY
Sbjct: 481 SGYYKRDDLTAEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY 540

Query: 541 GLVTDIEMIWIYGNSFESFLVAVVNPKKQSLEHWAEENGIKGDFNALCEDKRAKDYILGQ 600
           GLV+DIEMIWIYGNSFESFLVAVVNPKKQ+LE WAEENGIKGDF+ALCEDKRAKDYILGQ
Sbjct: 541 GLVSDIEMIWIYGNSFESFLVAVVNPKKQALEQWAEENGIKGDFDALCEDKRAKDYILGQ 600

Query: 601 LSKIAKEKKLKGYENIRAVHLDPLPFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGN 660
           LSKIAKEKKLKGYENIRAVHLDPLPFDIERDL+TPSFKKKRPQLLKYYQKEID+MYKSGN
Sbjct: 601 LSKIAKEKKLKGYENIRAVHLDPLPFDIERDLMTPSFKKKRPQLLKYYQKEIDEMYKSGN 660

Query: 661 KPVA 665
           KPVA
Sbjct: 661 KPVA 664

BLAST of HG10009056 vs. NCBI nr
Match: KAA0042684.1 (long chain acyl-CoA synthetase 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 1291.2 bits (3340), Expect = 0.0e+00
Identity = 630/689 (91.44%), Postives = 649/689 (94.19%), Query Frame = 0

Query: 1   MQRKYLVQVEEAKEAAAGRPSVGPVYRSIFAKDGFPPPVQGLETCWDIFRMSVEKNPGNR 60
           MQRKYLVQVEEAK+A AGRPSVGPVYRSIFAKDGFPPPVQGL+TCWDIFRMSVEKNPGNR
Sbjct: 1   MQRKYLVQVEEAKDATAGRPSVGPVYRSIFAKDGFPPPVQGLDTCWDIFRMSVEKNPGNR 60

Query: 61  MLGRRKIVEGNPAKAGECEWLTYKEVYELVLKIGNAMRGLGYGPGVKCGIYGANCPEWII 120
           MLGRRK+VEGNPAKAGE EWLTYKEVYELVLKIGNAMRGLGYGPG KCGIYGANCPEWII
Sbjct: 61  MLGRRKVVEGNPAKAGEYEWLTYKEVYELVLKIGNAMRGLGYGPGEKCGIYGANCPEWII 120

Query: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAEISISFVEEKKISELLKTLPNTTKFLKTV 180
           SMEACNAHGLYCVPLYDTLGAGAIEFII HAEISI+FVEEKKISELLKTLPNT KFLKT+
Sbjct: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIISHAEISIAFVEEKKISELLKTLPNTAKFLKTI 180

Query: 181 VSFGKVSDNHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPK 240
           VSFGKVSDNHKEEV KFGLEIY+WEEFLQKGDSQQ+DLPVK K DICTIMYTSGTTGDPK
Sbjct: 181 VSFGKVSDNHKEEVKKFGLEIYTWEEFLQKGDSQQFDLPVKEKSDICTIMYTSGTTGDPK 240

Query: 241 GVLLSNNAIIALIAGVKHLLTTVKEQVQENDVYISYLPLAHIFDRVIEEVFILNSASIGF 300
           GVLLSNNAIIALIAGVKHLLTTV E VQENDVYISYLPLAHIFDRVIEEVFILN ASIGF
Sbjct: 241 GVLLSNNAIIALIAGVKHLLTTVNENVQENDVYISYLPLAHIFDRVIEEVFILNGASIGF 300

Query: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGFLKKSLFNFAYSYKHNQM 360
           WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGFLK+SLFN AYSYK+NQM
Sbjct: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGFLKRSLFNLAYSYKYNQM 360

Query: 361 QKGKKHEEAAPLFDKVVFDKVKKGLGGKVRIILSGGAPLASHIETFFRVVSCAHVLQGY- 420
           QKGKKH++AAPLFDKVVFDKVKKGLGG VRIILSGGAPLA+HIETFFRVVSCAHVLQGY 
Sbjct: 361 QKGKKHQQAAPLFDKVVFDKVKKGLGGNVRIILSGGAPLAAHIETFFRVVSCAHVLQGYG 420

Query: 421 ------------------------GLTETCGGTFVTLPNELPMLGTVGPPVPNVDVCLES 480
                                   GLTETCGGTFVTLP+ELPMLGTVGPPVPNVD+CLES
Sbjct: 421 ISSLYLHFQKPKQREPKYHTFLFAGLTETCGGTFVTLPDELPMLGTVGPPVPNVDICLES 480

Query: 481 VPEMGYDSHASTPRGEVCIKGDTLFSGYYKRDDLTTEVLVDGWFHTGDVGEWQPDGSLKI 540
           VPEMGYDSHASTPRGEVCIKGDTLFSGYYKRDDLTTEVLVDGWFHTGDVGEWQPDGSLKI
Sbjct: 481 VPEMGYDSHASTPRGEVCIKGDTLFSGYYKRDDLTTEVLVDGWFHTGDVGEWQPDGSLKI 540

Query: 541 IDRKKNIFKLSQGEYVAVENLELIYGLVTDIEMIWIYGNSFESFLVAVVNPKKQSLEHWA 600
           IDRKKNIFKLSQGEYVAVENLELIYGLV+DIEMIWIYGNSFESFLVAVVNPKKQ+LE WA
Sbjct: 541 IDRKKNIFKLSQGEYVAVENLELIYGLVSDIEMIWIYGNSFESFLVAVVNPKKQALEQWA 600

Query: 601 EENGIKGDFNALCEDKRAKDYILGQLSKIAKEKKLKGYENIRAVHLDPLPFDIERDLLTP 660
           EENGIK DFNALCEDKRAKDYILGQLSKIAKE+KLKGYENIRAVHLDPLPFDIERDLLTP
Sbjct: 601 EENGIKEDFNALCEDKRAKDYILGQLSKIAKEQKLKGYENIRAVHLDPLPFDIERDLLTP 660

Query: 661 SFKKKRPQLLKYYQKEIDDMYKSGNKPVA 665
           SFKKKRPQLLKYYQK+IDDMYKSGNKP+A
Sbjct: 661 SFKKKRPQLLKYYQKKIDDMYKSGNKPIA 689

BLAST of HG10009056 vs. NCBI nr
Match: XP_023549978.1 (long chain acyl-CoA synthetase 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1283.5 bits (3320), Expect = 0.0e+00
Identity = 620/664 (93.37%), Postives = 643/664 (96.84%), Query Frame = 0

Query: 1   MQRKYLVQVEEAKEAAAGRPSVGPVYRSIFAKDGFPPPVQGLETCWDIFRMSVEKNPGNR 60
           MQRKYLVQVEE KEAAAGRPSVGPVYRS+FAKDGFPP VQGLETCWDIFRMSVEKNPGNR
Sbjct: 1   MQRKYLVQVEEGKEAAAGRPSVGPVYRSVFAKDGFPPSVQGLETCWDIFRMSVEKNPGNR 60

Query: 61  MLGRRKIVEGNPAKAGECEWLTYKEVYELVLKIGNAMRGLGYGPGVKCGIYGANCPEWII 120
           MLGRRKI+EGN AKAGE EWLTY++VYELVLKIGNAMR LGYGPG KCGIYGANCPEWII
Sbjct: 61  MLGRRKIIEGNLAKAGEYEWLTYQQVYELVLKIGNAMRSLGYGPGEKCGIYGANCPEWII 120

Query: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAEISISFVEEKKISELLKTLPNTTKFLKTV 180
           SMEACNAHGLYCVPLYDTLGAGAIEFIICHAE SI+FVEEKKI ELLKTLPNT   LKT+
Sbjct: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAETSIAFVEEKKIPELLKTLPNTANVLKTL 180

Query: 181 VSFGKVSDNHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPK 240
           VSFGKVS+NHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPK
Sbjct: 181 VSFGKVSNNHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPK 240

Query: 241 GVLLSNNAIIALIAGVKHLLTTVKEQVQENDVYISYLPLAHIFDRVIEEVFILNSASIGF 300
           GVLLSN AIIALIAGVKHLL TVKE+VQENDVYISYLPLAHIFDRVIEEVFILN ASIGF
Sbjct: 241 GVLLSNKAIIALIAGVKHLLATVKEEVQENDVYISYLPLAHIFDRVIEEVFILNGASIGF 300

Query: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGFLKKSLFNFAYSYKHNQM 360
           WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGG LK+SLFNFAYSYK+NQM
Sbjct: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGLLKRSLFNFAYSYKYNQM 360

Query: 361 QKGKKHEEAAPLFDKVVFDKVKKGLGGKVRIILSGGAPLASHIETFFRVVSCAHVLQGYG 420
           QKGKKHE+AA LFDK+VFDKVKKGLGGKVRIILSG APLA+HIETFFRVVSCAHVLQGYG
Sbjct: 361 QKGKKHEQAATLFDKLVFDKVKKGLGGKVRIILSGAAPLAAHIETFFRVVSCAHVLQGYG 420

Query: 421 LTETCGGTFVTLPNELPMLGTVGPPVPNVDVCLESVPEMGYDSHASTPRGEVCIKGDTLF 480
           LTETCGGTFVTLPNEL MLGTVGPPVPNVD+CLESVPEMGYDS ASTPRGEVCIKGDTLF
Sbjct: 421 LTETCGGTFVTLPNELRMLGTVGPPVPNVDICLESVPEMGYDSLASTPRGEVCIKGDTLF 480

Query: 481 SGYYKRDDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY 540
           +GYYKR+DLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY
Sbjct: 481 AGYYKREDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY 540

Query: 541 GLVTDIEMIWIYGNSFESFLVAVVNPKKQSLEHWAEENGIKGDFNALCEDKRAKDYILGQ 600
           GLV+DIEMIWIYGNSFE+FLVAVVNP++Q+LEHWAEENGIKGDFNALCEDKRAKDYILGQ
Sbjct: 541 GLVSDIEMIWIYGNSFEAFLVAVVNPQRQALEHWAEENGIKGDFNALCEDKRAKDYILGQ 600

Query: 601 LSKIAKEKKLKGYENIRAVHLDPLPFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGN 660
           LSKIAKEKKLKGYE I+AVHLDPLPFDIERDLLTPSFKKKRPQ+LKYYQKEIDDMYK GN
Sbjct: 601 LSKIAKEKKLKGYEVIKAVHLDPLPFDIERDLLTPSFKKKRPQMLKYYQKEIDDMYKGGN 660

Query: 661 KPVA 665
           KPVA
Sbjct: 661 KPVA 664

BLAST of HG10009056 vs. ExPASy Swiss-Prot
Match: Q9T0A0 (Long chain acyl-CoA synthetase 4 OS=Arabidopsis thaliana OX=3702 GN=LACS4 PE=2 SV=1)

HSP 1 Score: 1008.8 bits (2607), Expect = 2.8e-293
Identity = 468/663 (70.59%), Postives = 565/663 (85.22%), Query Frame = 0

Query: 2   QRKYLVQVEEAKEAAAGRPSVGPVYRSIFAKDGFPPPVQGLETCWDIFRMSVEKNPGNRM 61
           Q+KY+ QVEE KE + GRPSVGPVYRSIFAKDGFP P++G+++CWD+FRMSVEK P N M
Sbjct: 4   QKKYIFQVEEGKEGSDGRPSVGPVYRSIFAKDGFPDPIEGMDSCWDVFRMSVEKYPNNPM 63

Query: 62  LGRRKIVEGNPAKAGECEWLTYKEVYELVLKIGNAMRGLGYGPGVKCGIYGANCPEWIIS 121
           LGRR+IV+G P   G+  W TY+EVY++V+K+GN++R +G     KCGIYGAN PEWIIS
Sbjct: 64  LGRREIVDGKP---GKYVWQTYQEVYDIVMKLGNSLRSVGVKDEAKCGIYGANSPEWIIS 123

Query: 122 MEACNAHGLYCVPLYDTLGAGAIEFIICHAEISISFVEEKKISELLKTLPNTTKFLKTVV 181
           MEACNAHGLYCVPLYDTLGA A+EFII H+E+SI FVEEKKISEL KT PN+T+++KTVV
Sbjct: 124 MEACNAHGLYCVPLYDTLGADAVEFIISHSEVSIVFVEEKKISELFKTCPNSTEYMKTVV 183

Query: 182 SFGKVSDNHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPKG 241
           SFG VS   KEE   FGL IY+W+EFL+ G+ +QYDLP+K K DICTIMYTSGTTGDPKG
Sbjct: 184 SFGGVSREQKEEAETFGLVIYAWDEFLKLGEGKQYDLPIKKKSDICTIMYTSGTTGDPKG 243

Query: 242 VLLSNNAIIALIAGVKHLLTTVKEQVQENDVYISYLPLAHIFDRVIEEVFILNSASIGFW 301
           V++SN +I+ LIAGV  LL +  E +   DVY+SYLPLAHIFDRVIEE FI + A+IGFW
Sbjct: 244 VMISNESIVTLIAGVIRLLKSANEALTVKDVYLSYLPLAHIFDRVIEECFIQHGAAIGFW 303

Query: 302 RGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGFLKKSLFNFAYSYKHNQMQ 361
           RGDVKLL+ED+ ELKPTIFCAVPRVL+R+Y GL +K+S GGFLKK +F+ A+SYK   M+
Sbjct: 304 RGDVKLLIEDLAELKPTIFCAVPRVLDRVYSGLQKKLSDGGFLKKFIFDSAFSYKFGYMK 363

Query: 362 KGKKHEEAAPLFDKVVFDKVKKGLGGKVRIILSGGAPLASHIETFFRVVSCAHVLQGYGL 421
           KG+ H EA+PLFDK+VF KVK+GLGG VRIILSG APLASH+E+F RVV+C HVLQGYGL
Sbjct: 364 KGQSHVEASPLFDKLVFSKVKQGLGGNVRIILSGAAPLASHVESFLRVVACCHVLQGYGL 423

Query: 422 TETCGGTFVTLPNELPMLGTVGPPVPNVDVCLESVPEMGYDSHASTPRGEVCIKGDTLFS 481
           TE+C GTFV+LP+EL MLGTVGPPVPNVD+ LESVPEM YD+ AST RGE+CI+G TLFS
Sbjct: 424 TESCAGTFVSLPDELGMLGTVGPPVPNVDIRLESVPEMEYDALASTARGEICIRGKTLFS 483

Query: 482 GYYKRDDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIYG 541
           GYYKR+DLT EVL+DGW HTGDVGEWQPDGS+KIIDRKKNIFKLSQGEYVAVEN+E IYG
Sbjct: 484 GYYKREDLTKEVLIDGWLHTGDVGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIYG 543

Query: 542 LVTDIEMIWIYGNSFESFLVAVVNPKKQSLEHWAEENGIKGDFNALCEDKRAKDYILGQL 601
            V  ++ +W+YGNSFESFL+A+ NP +  LE WA ENG+ GD++ALC++++AK++ILG+L
Sbjct: 544 EVQAVDSVWVYGNSFESFLIAIANPNQHILERWAAENGVSGDYDALCQNEKAKEFILGEL 603

Query: 602 SKIAKEKKLKGYENIRAVHLDPLPFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGNK 661
            K+AKEKK+KG+E I+A+HLDP+PFD+ERDLLTP+FKKKRPQLLKYYQ  ID+MYK+ N 
Sbjct: 604 VKMAKEKKMKGFEIIKAIHLDPVPFDMERDLLTPTFKKKRPQLLKYYQSVIDEMYKTINA 663

Query: 662 PVA 665
             A
Sbjct: 664 KFA 663

BLAST of HG10009056 vs. ExPASy Swiss-Prot
Match: Q9C7W4 (Long chain acyl-CoA synthetase 3 OS=Arabidopsis thaliana OX=3702 GN=LACS3 PE=2 SV=1)

HSP 1 Score: 983.4 bits (2541), Expect = 1.3e-285
Identity = 454/659 (68.89%), Postives = 557/659 (84.52%), Query Frame = 0

Query: 4   KYLVQVEEAKEAA-AGRPSVGPVYRSIFAKDGFPPPVQGLETCWDIFRMSVEKNPGNRML 63
           +Y+V+VE+ K+    G PSVGPVYRSI+AKDGFP P   L + WDIFR+SVEK+P N ML
Sbjct: 5   RYIVEVEKGKQGVDGGSPSVGPVYRSIYAKDGFPEPPDDLVSAWDIFRLSVEKSPNNPML 64

Query: 64  GRRKIVEGNPAKAGECEWLTYKEVYELVLKIGNAMRGLGYGPGVKCGIYGANCPEWIISM 123
           GRR+IV+G   KAG+  W TYKEV+ +V+K+GN++R +G G G KCGIYGAN PEWIISM
Sbjct: 65  GRREIVDG---KAGKYVWQTYKEVHNVVIKLGNSIRTIGVGKGDKCGIYGANSPEWIISM 124

Query: 124 EACNAHGLYCVPLYDTLGAGAIEFIICHAEISISFVEEKKISELLKTLPNTTKFLKTVVS 183
           EACNAHGLYCVPLYDTLGAGAIEFIICHAE+S++F EE KISELLKT P +TK+LK +VS
Sbjct: 125 EACNAHGLYCVPLYDTLGAGAIEFIICHAEVSLAFAEENKISELLKTAPKSTKYLKYIVS 184

Query: 184 FGKVSDNHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPKGV 243
           FG+V++N + E  +  L IYSW++FL+ G+ + Y+LP K + D+CTIMYTSGTTGDPKGV
Sbjct: 185 FGEVTNNQRVEAERHRLTIYSWDQFLKLGEGKHYELPEKRRSDVCTIMYTSGTTGDPKGV 244

Query: 244 LLSNNAIIALIAGVKHLLTTVKEQVQENDVYISYLPLAHIFDRVIEEVFILNSASIGFWR 303
           LL+N +II L+ GVK LL T+ E++   DVY+SYLPLAHIFDRVIEE+ I  +ASIGFWR
Sbjct: 245 LLTNESIIHLLEGVKKLLKTIDEELTSKDVYLSYLPLAHIFDRVIEELCIYEAASIGFWR 304

Query: 304 GDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGFLKKSLFNFAYSYKHNQMQK 363
           GDVK+L+EDI  LKPT+FCAVPRVLERIY GL QK+S GGF+KK LFNFA+ YKH  M+K
Sbjct: 305 GDVKILIEDIAALKPTVFCAVPRVLERIYTGLQQKLSDGGFVKKKLFNFAFKYKHKNMEK 364

Query: 364 GKKHEEAAPLFDKVVFDKVKKGLGGKVRIILSGGAPLASHIETFFRVVSCAHVLQGYGLT 423
           G+ HE+A+P+ DK+VF KVK+GLGG VR+ILSG APLA+HIE+F RVV+CAHVLQGYGLT
Sbjct: 365 GQPHEQASPIADKIVFKKVKEGLGGNVRLILSGAAPLAAHIESFLRVVACAHVLQGYGLT 424

Query: 424 ETCGGTFVTLPNELPMLGTVGPPVPNVDVCLESVPEMGYDSHASTPRGEVCIKGDTLFSG 483
           E+CGGTFV++PNEL MLGTVGPPVPNVD+ LESVPEMGYD+ AS PRGE+CI+G TLFSG
Sbjct: 425 ESCGGTFVSIPNELSMLGTVGPPVPNVDIRLESVPEMGYDALASNPRGEICIRGKTLFSG 484

Query: 484 YYKRDDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIYGL 543
           YYKR+DLT EV +DGW HTGDVGEWQPDG++KIIDRKKNIFKLSQGEYVAVENLE IY  
Sbjct: 485 YYKREDLTQEVFIDGWLHTGDVGEWQPDGAMKIIDRKKNIFKLSQGEYVAVENLENIYSH 544

Query: 544 VTDIEMIWIYGNSFESFLVAVVNPKKQSLEHWAEENGIKGDFNALCEDKRAKDYILGQLS 603
           V  IE IW+YGNS+ES+LVAVV P K  +EHWA+E+ + GDF ++C +++ K+++LG+ +
Sbjct: 545 VAAIESIWVYGNSYESYLVAVVCPSKIQIEHWAKEHKVSGDFESICRNQKTKEFVLGEFN 604

Query: 604 KIAKEKKLKGYENIRAVHLDPLPFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGNK 662
           ++AK+KKLKG+E I+ VHLD +PFD+ERDL+TPS+K KRPQLLKYYQKEID+MYK   +
Sbjct: 605 RVAKDKKLKGFELIKGVHLDTVPFDMERDLITPSYKMKRPQLLKYYQKEIDEMYKKNRE 660

BLAST of HG10009056 vs. ExPASy Swiss-Prot
Match: Q9T009 (Long chain acyl-CoA synthetase 5 OS=Arabidopsis thaliana OX=3702 GN=LACS5 PE=2 SV=1)

HSP 1 Score: 966.8 bits (2498), Expect = 1.2e-280
Identity = 450/663 (67.87%), Postives = 543/663 (81.90%), Query Frame = 0

Query: 2   QRKYLVQVEEAKEAAAGRPSVGPVYRSIFAKDGFPPPVQGLETCWDIFRMSVEKNPGNRM 61
           Q++++ +VE AKEA  G PSVGPVYRS FA++GFP P+ G+++CWDIFR +VEK P NRM
Sbjct: 4   QKRFIFEVEAAKEATDGNPSVGPVYRSTFAQNGFPNPIDGIQSCWDIFRTAVEKYPNNRM 63

Query: 62  LGRRKIVEGNPAKAGECEWLTYKEVYELVLKIGNAMRGLGYGPGVKCGIYGANCPEWIIS 121
           LGRR+I  G   KAG+  W TYKEVY++V+K+GN++R  G   G KCGIYG NC EWIIS
Sbjct: 64  LGRREISNG---KAGKYVWKTYKEVYDIVIKLGNSLRSCGIKEGEKCGIYGINCCEWIIS 123

Query: 122 MEACNAHGLYCVPLYDTLGAGAIEFIICHAEISISFVEEKKISELLKTLPNTTKFLKTVV 181
           MEACNAHGLYCVPLYDTLGAGA+EFII HAE+SI+FVEEKKI EL KT PN+TK++KTVV
Sbjct: 124 MEACNAHGLYCVPLYDTLGAGAVEFIISHAEVSIAFVEEKKIPELFKTCPNSTKYMKTVV 183

Query: 182 SFGKVSDNHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPKG 241
           SFG V    KEE  K GL I+SW+EFL+ G+ +QY+LP+K   DICTIMYTSGTTGDPKG
Sbjct: 184 SFGGVKPEQKEEAEKLGLVIHSWDEFLKLGEGKQYELPIKKPSDICTIMYTSGTTGDPKG 243

Query: 242 VLLSNNAIIALIAGVKHLLTTVKEQVQENDVYISYLPLAHIFDRVIEEVFILNSASIGFW 301
           V++SN +I+ +  GV H L  V   + E DVYISYLPLAH+FDR IEE  I    SIGFW
Sbjct: 244 VMISNESIVTITTGVMHFLGNVNASLSEKDVYISYLPLAHVFDRAIEECIIQVGGSIGFW 303

Query: 302 RGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGFLKKSLFNFAYSYKHNQMQ 361
           RGDVKLL+ED+GELKP+IFCAVPRVL+R+Y GL QK+S GGF KK +F+ A+SYK   M+
Sbjct: 304 RGDVKLLIEDLGELKPSIFCAVPRVLDRVYTGLQQKLSGGGFFKKKVFDVAFSYKFGNMK 363

Query: 362 KGKKHEEAAPLFDKVVFDKVKKGLGGKVRIILSGGAPLASHIETFFRVVSCAHVLQGYGL 421
           KG+ H  A+P  DK+VF+KVK+GLGG VRIILSG APLASHIE+F RVV+C +VLQGYGL
Sbjct: 364 KGQSHVAASPFCDKLVFNKVKQGLGGNVRIILSGAAPLASHIESFLRVVACCNVLQGYGL 423

Query: 422 TETCGGTFVTLPNELPMLGTVGPPVPNVDVCLESVPEMGYDSHASTPRGEVCIKGDTLFS 481
           TE+C GTF T P+EL MLGTVGPPVPNVD+ LESVPEM YD+  STPRGE+CI+G TLFS
Sbjct: 424 TESCAGTFATFPDELDMLGTVGPPVPNVDIRLESVPEMNYDALGSTPRGEICIRGKTLFS 483

Query: 482 GYYKRDDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIYG 541
           GYYKR+DLT EV +DGW HTGDVGEWQP+GS+KIIDRKKNIFKL+QGEYVAVENLE +Y 
Sbjct: 484 GYYKREDLTKEVFIDGWLHTGDVGEWQPNGSMKIIDRKKNIFKLAQGEYVAVENLENVYS 543

Query: 542 LVTDIEMIWIYGNSFESFLVAVVNPKKQSLEHWAEENGIKGDFNALCEDKRAKDYILGQL 601
            V  IE IW+YGNSFESFLVA+ NP +Q+LE WA ENG+ GDFN++C++ +AK +ILG+L
Sbjct: 544 QVEVIESIWVYGNSFESFLVAIANPAQQTLERWAVENGVNGDFNSICQNAKAKAFILGEL 603

Query: 602 SKIAKEKKLKGYENIRAVHLDPLPFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGNK 661
            K AKE KLKG+E I+ VHL+P+ FD+ERDLLTP++KKKRPQLLKYYQ  I +MYK+  +
Sbjct: 604 VKTAKENKLKGFEIIKDVHLEPVAFDMERDLLTPTYKKKRPQLLKYYQNVIHEMYKTTKE 663

Query: 662 PVA 665
            +A
Sbjct: 664 SLA 663

BLAST of HG10009056 vs. ExPASy Swiss-Prot
Match: M4ISH1 (Probable CoA ligase CCL6 OS=Humulus lupulus OX=3486 GN=CCL6 PE=2 SV=1)

HSP 1 Score: 777.7 bits (2007), Expect = 1.0e-223
Identity = 358/657 (54.49%), Postives = 486/657 (73.97%), Query Frame = 0

Query: 5   YLVQVEEAKEAAAGRPSVGPVYRSIFAKDGFPPPVQGLETCWDIFRMSVEKNPGNRMLGR 64
           Y V+VEEA+ A    PS GPVYRSI+A+DG     +GL++ W+    SV+++P   MLGR
Sbjct: 3   YTVKVEEARPATEKMPSAGPVYRSIYARDGLLELPEGLQSPWEFLSGSVKRSPKTPMLGR 62

Query: 65  RKIVEGNPAKAGECEWLTYKEVYELVLKIGNAMRGLGYGPGVKCGIYGANCPEWIISMEA 124
           R+I +   ++AG   WLTY+EV++  +++ +AMR  G  PG +CGIYG NCP+WI++M+A
Sbjct: 63  RQIKD---SEAGPYVWLTYQEVHDEAIRMASAMRSRGVNPGDRCGIYGTNCPQWIVAMQA 122

Query: 125 CNAHGLYCVPLYDTLGAGAIEFIICHAEISISFVEEKKISELLKTLPNTTKFLKTVVSFG 184
           C +H +  VPLYDTLG  A+EFII H E+SI+FV+E KIS +L  LPN +  LKT+VSFG
Sbjct: 123 CYSHAITYVPLYDTLGPNAVEFIINHGEVSIAFVQENKISAILSCLPNCSSLLKTIVSFG 182

Query: 185 KVSDNHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPKGVLL 244
            ++   K+E    G+  +SWEEF Q G+    +LP K + DICT+MYTSG TG+PKGV+L
Sbjct: 183 NITSVQKKETEALGVSCFSWEEFSQLGNLSG-ELPEKHRTDICTLMYTSGATGEPKGVIL 242

Query: 245 SNNAIIALIAGVKHLLTTVKEQVQENDVYISYLPLAHIFDRVIEEVFILNSASIGFWRGD 304
           +N AI+A I    ++L    +   E DVY SYLPLAH++D+++E   I   ++IG+WRGD
Sbjct: 243 TNEAIMAEILSTDNMLELTDKVFSEEDVYFSYLPLAHVYDQIVENYCIYKGSAIGYWRGD 302

Query: 305 VKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGFLKKSLFNFAYSYKHNQMQKGK 364
           ++ L++D+ ELKPTIFC VPRV +RIY G+  K+SSGG LKK LF +AY+YK   M+KG 
Sbjct: 303 IRFLMDDLQELKPTIFCGVPRVYDRIYAGIFHKVSSGGTLKKMLFQYAYNYKMANMEKGL 362

Query: 365 KHEEAAPLFDKVVFDKVKKGLGGKVRIILSGGAPLASHIETFFRVVSCAHVLQGYGLTET 424
            H +AAPL DK+ FDK+K+G GG++R++ SG APL  H+E + RV SCA + QGYGLTE+
Sbjct: 363 PHGQAAPLMDKLFFDKIKQGFGGRIRLMFSGAAPLPHHVEEYLRVTSCAALSQGYGLTES 422

Query: 425 CGGTFVTLPNELPMLGTVGPPVPNVDVCLESVPEMGYDSHASTPRGEVCIKGDTLFSGYY 484
           CGG   ++ N  PM+GTVG P+  ++  LESVPEMGYD+ +  PRGE+C++G TLFSGY+
Sbjct: 423 CGGCLTSIANIFPMIGTVGVPMTTIEARLESVPEMGYDALSDKPRGEICLRGTTLFSGYH 482

Query: 485 KRDDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIYGLVT 544
           KR+DLT +VLVDGWFHTGD+GEWQP+G++KIIDRKKNIFKLSQGEYV VEN+E IY    
Sbjct: 483 KREDLTKDVLVDGWFHTGDIGEWQPNGAMKIIDRKKNIFKLSQGEYVPVENIEGIYLQCP 542

Query: 545 DIEMIWIYGNSFESFLVAVVNPKKQSLEHWAEENGIKGDFNALCEDKRAKDYILGQLSKI 604
            I  IW+YGNSFESFLVAVV P++ +LE+WA    +  DF +LCE+ +A  YIL +L+  
Sbjct: 543 LIASIWVYGNSFESFLVAVVVPERLALENWAANRNLTDDFKSLCENPKASKYILDELNST 602

Query: 605 AKEKKLKGYENIRAVHLDPLPFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGNK 662
           AK+ +L+G+E ++AV+L+P PFD+ERDL+TP+FK KRPQLLKYY+  +D +Y    +
Sbjct: 603 AKKHQLRGFEMLKAVYLEPNPFDMERDLITPTFKLKRPQLLKYYKDHVDKLYSEAKE 655

BLAST of HG10009056 vs. ExPASy Swiss-Prot
Match: Q9XIA9 (Long chain acyl-CoA synthetase 2 OS=Arabidopsis thaliana OX=3702 GN=LACS2 PE=2 SV=1)

HSP 1 Score: 734.6 bits (1895), Expect = 1.0e-210
Identity = 341/658 (51.82%), Postives = 470/658 (71.43%), Query Frame = 0

Query: 9   VEEAKEAAAGRPSVGPVYRSIFAKDGFPPPVQGLETCWDIFRMSVEKNPGNRMLGRRKIV 68
           VEE + A A  PS GPVYR  +AKDG       +++ W  F  +V+K P  +MLG+R   
Sbjct: 11  VEEGRPATAEHPSAGPVYRCKYAKDGLLDLPTDIDSPWQFFSEAVKKYPNEQMLGQRVTT 70

Query: 69  EGNPAKAGECEWLTYKEVYELVLKIGNAMRGLGYGPGVKCGIYGANCPEWIISMEACNAH 128
           +   +K G   W+TYKE ++  ++IG+A+R  G  PG  CGIYGANCPEWII+MEAC + 
Sbjct: 71  D---SKVGPYTWITYKEAHDAAIRIGSAIRSRGVDPGHCCGIYGANCPEWIIAMEACMSQ 130

Query: 129 GLYCVPLYDTLGAGAIEFIICHAEISISFVEEKKISELLKTLPNTTKFLKTVVSFGKVSD 188
           G+  VPLYD+LG  A+EFII HAE+S+ FV+EK +S +L      +  LKT+VSFG+VS 
Sbjct: 131 GITYVPLYDSLGVNAVEFIINHAEVSLVFVQEKTVSSILSCQKGCSSNLKTIVSFGEVSS 190

Query: 189 NHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPKGVLLSNNA 248
             KEE     + ++SW EF   G+  + +LP K K DICTIMYTSGTTG+PKGV+L+N A
Sbjct: 191 TQKEEAKNQCVSLFSWNEFSLMGNLDEANLPRKRKTDICTIMYTSGTTGEPKGVILNNAA 250

Query: 249 IIALIAGVKHLLTTVKEQVQENDVYISYLPLAHIFDRVIEEVFILNSASIGFWRGDVKLL 308
           I   +  +  +L         +DV+ SYLPLAH +D+V+E  F+   +S+G+WRGD++ L
Sbjct: 251 ISVQVLSIDKMLEVTDRSCDTSDVFFSYLPLAHCYDQVMEIYFLSRGSSVGYWRGDIRYL 310

Query: 309 VEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGFLKKSLFNFAYSYKHNQMQKGKKHEE 368
           ++D+  LKPT+FC VPRV +++Y G+ QKIS+ G ++K LF+FAY+YK   M+KG   EE
Sbjct: 311 MDDVQALKPTVFCGVPRVYDKLYAGIMQKISASGLIRKKLFDFAYNYKLGNMRKGFSQEE 370

Query: 369 AAPLFDKVVFDKVKKGLGGKVRIILSGGAPLASHIETFFRVVSCAHVLQGYGLTETCGGT 428
           A+P  D+++FDK+K+ LGG+  ++LSG APL  H+E F R++  +++ QGYGLTE+CGG+
Sbjct: 371 ASPRLDRLMFDKIKEALGGRAHMLLSGAAPLPRHVEEFLRIIPASNLSQGYGLTESCGGS 430

Query: 429 FVTLPNELPMLGTVGPPVPNVDVCLESVPEMGYDS-HASTPRGEVCIKGDTLFSGYYKRD 488
           F TL     M+GTVG P+P V+  L SVPEMGYD+  A  PRGE+C++G+++FSGY+KR 
Sbjct: 431 FTTLAGVFSMVGTVGVPMPTVEARLVSVPEMGYDAFSADVPRGEICLRGNSMFSGYHKRQ 490

Query: 489 DLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIYGLVTDIE 548
           DLT +VL+DGWFHTGD+GEWQ DGS+KIIDRKKNIFKLSQGEYVAVENLE  Y     I 
Sbjct: 491 DLTDQVLIDGWFHTGDIGEWQEDGSMKIIDRKKNIFKLSQGEYVAVENLENTYSRCPLIA 550

Query: 549 MIWIYGNSFESFLVAVVNPKKQSLEHWAEEN-GIKGDFNALCEDKRAKDYILGQLSKIAK 608
            IW+YGNSFESFLV VV P ++++E WA+ N     DF +LC++ +A+ Y L +L+  AK
Sbjct: 551 QIWVYGNSFESFLVGVVVPDRKAIEDWAKLNYQSPNDFESLCQNLKAQKYFLDELNSTAK 610

Query: 609 EKKLKGYENIRAVHLDPLPFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGNKPVA 665
           + +LKG+E ++A+HL+P PFDIERDL+TP+FK KRPQLL++Y+  +D +Y    + +A
Sbjct: 611 QYQLKGFEMLKAIHLEPNPFDIERDLITPTFKLKRPQLLQHYKGIVDQLYSEAKRSMA 665

BLAST of HG10009056 vs. ExPASy TrEMBL
Match: A0A5D3C2P4 (Long chain acyl-CoA synthetase 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G001880 PE=4 SV=1)

HSP 1 Score: 1306.6 bits (3380), Expect = 0.0e+00
Identity = 631/664 (95.03%), Postives = 649/664 (97.74%), Query Frame = 0

Query: 1   MQRKYLVQVEEAKEAAAGRPSVGPVYRSIFAKDGFPPPVQGLETCWDIFRMSVEKNPGNR 60
           MQRKYLVQVEEAK+A AGRPSVGPVYRSIFAKDGFPPPVQGL+TCWDIFRMSVEKNPGNR
Sbjct: 1   MQRKYLVQVEEAKDATAGRPSVGPVYRSIFAKDGFPPPVQGLDTCWDIFRMSVEKNPGNR 60

Query: 61  MLGRRKIVEGNPAKAGECEWLTYKEVYELVLKIGNAMRGLGYGPGVKCGIYGANCPEWII 120
           MLGRRK+VEGNPAKAGE EWLTYKEVYELVLKIGNAMRGLGYGPG KCGIYGANCPEWII
Sbjct: 61  MLGRRKVVEGNPAKAGEYEWLTYKEVYELVLKIGNAMRGLGYGPGEKCGIYGANCPEWII 120

Query: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAEISISFVEEKKISELLKTLPNTTKFLKTV 180
           SMEACNAHGLYCVPLYDTLGAGAIEFII HAEISI+FVEEKKISELLKTLPNT KFLKT+
Sbjct: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIISHAEISIAFVEEKKISELLKTLPNTAKFLKTI 180

Query: 181 VSFGKVSDNHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPK 240
           VSFGKVSDNHKEEV KFGLEIY+WEEFLQKGDSQQ+DLPVK K DICTIMYTSGTTGDPK
Sbjct: 181 VSFGKVSDNHKEEVKKFGLEIYTWEEFLQKGDSQQFDLPVKEKSDICTIMYTSGTTGDPK 240

Query: 241 GVLLSNNAIIALIAGVKHLLTTVKEQVQENDVYISYLPLAHIFDRVIEEVFILNSASIGF 300
           GVLLSNNAIIALIAGVKHLLTTV E VQENDVYISYLPLAHIFDRVIEEVFILN ASIGF
Sbjct: 241 GVLLSNNAIIALIAGVKHLLTTVNENVQENDVYISYLPLAHIFDRVIEEVFILNGASIGF 300

Query: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGFLKKSLFNFAYSYKHNQM 360
           WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGFLK+SLFN AYSYK+NQM
Sbjct: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGFLKRSLFNLAYSYKYNQM 360

Query: 361 QKGKKHEEAAPLFDKVVFDKVKKGLGGKVRIILSGGAPLASHIETFFRVVSCAHVLQGYG 420
           QKGKKH++AAPLFDKVVFDKVKKGLGG VRIILSGGAPLA+HIETFFRVVSCAHVLQGYG
Sbjct: 361 QKGKKHQQAAPLFDKVVFDKVKKGLGGNVRIILSGGAPLAAHIETFFRVVSCAHVLQGYG 420

Query: 421 LTETCGGTFVTLPNELPMLGTVGPPVPNVDVCLESVPEMGYDSHASTPRGEVCIKGDTLF 480
           LTETCGGTFVTLP+ELPMLGTVGPPVPNVD+CLESVPEMGYDSHASTPRGEVCIKGDTLF
Sbjct: 421 LTETCGGTFVTLPDELPMLGTVGPPVPNVDICLESVPEMGYDSHASTPRGEVCIKGDTLF 480

Query: 481 SGYYKRDDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY 540
           SGYYKRDDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY
Sbjct: 481 SGYYKRDDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY 540

Query: 541 GLVTDIEMIWIYGNSFESFLVAVVNPKKQSLEHWAEENGIKGDFNALCEDKRAKDYILGQ 600
           GLV+DIEMIWIYGNSFESFLVAVVNPKKQ+LE WAEENGIK DFNALCEDKRAKDYILGQ
Sbjct: 541 GLVSDIEMIWIYGNSFESFLVAVVNPKKQALEQWAEENGIKEDFNALCEDKRAKDYILGQ 600

Query: 601 LSKIAKEKKLKGYENIRAVHLDPLPFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGN 660
           LSKIAKE+KLKGYENIRAVHLDPLPFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGN
Sbjct: 601 LSKIAKEQKLKGYENIRAVHLDPLPFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGN 660

Query: 661 KPVA 665
           KP+A
Sbjct: 661 KPIA 664

BLAST of HG10009056 vs. ExPASy TrEMBL
Match: A0A1S3AUH9 (long chain acyl-CoA synthetase 4-like OS=Cucumis melo OX=3656 GN=LOC103482821 PE=4 SV=1)

HSP 1 Score: 1306.6 bits (3380), Expect = 0.0e+00
Identity = 631/664 (95.03%), Postives = 649/664 (97.74%), Query Frame = 0

Query: 1   MQRKYLVQVEEAKEAAAGRPSVGPVYRSIFAKDGFPPPVQGLETCWDIFRMSVEKNPGNR 60
           MQRKYLVQVEEAK+A AGRPSVGPVYRSIFAKDGFPPPVQGL+TCWDIFRMSVEKNPGNR
Sbjct: 1   MQRKYLVQVEEAKDATAGRPSVGPVYRSIFAKDGFPPPVQGLDTCWDIFRMSVEKNPGNR 60

Query: 61  MLGRRKIVEGNPAKAGECEWLTYKEVYELVLKIGNAMRGLGYGPGVKCGIYGANCPEWII 120
           MLGRRK+VEGNPAKAGE EWLTYKEVYELVLKIGNAMRGLGYGPG KCGIYGANCPEWII
Sbjct: 61  MLGRRKVVEGNPAKAGEYEWLTYKEVYELVLKIGNAMRGLGYGPGEKCGIYGANCPEWII 120

Query: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAEISISFVEEKKISELLKTLPNTTKFLKTV 180
           SMEACNAHGLYCVPLYDTLGAGAIEFII HAEISI+FVEEKKISELLKTLPNT KFLKT+
Sbjct: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIISHAEISIAFVEEKKISELLKTLPNTAKFLKTI 180

Query: 181 VSFGKVSDNHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPK 240
           VSFGKVSDNHKEEV KFGLEIY+WEEFLQKGDSQQ+DLPVK K DICTIMYTSGTTGDPK
Sbjct: 181 VSFGKVSDNHKEEVKKFGLEIYTWEEFLQKGDSQQFDLPVKEKSDICTIMYTSGTTGDPK 240

Query: 241 GVLLSNNAIIALIAGVKHLLTTVKEQVQENDVYISYLPLAHIFDRVIEEVFILNSASIGF 300
           GVLLSNNAIIALIAGVKHLLTTV E VQENDVYISYLPLAHIFDRVIEEVFILN ASIGF
Sbjct: 241 GVLLSNNAIIALIAGVKHLLTTVNENVQENDVYISYLPLAHIFDRVIEEVFILNGASIGF 300

Query: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGFLKKSLFNFAYSYKHNQM 360
           WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGFLK+SLFN AYSYK+NQM
Sbjct: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGFLKRSLFNLAYSYKYNQM 360

Query: 361 QKGKKHEEAAPLFDKVVFDKVKKGLGGKVRIILSGGAPLASHIETFFRVVSCAHVLQGYG 420
           QKGKKH++AAPLFDKVVFDKVKKGLGG VRIILSGGAPLA+HIETFFRVVSCAHVLQGYG
Sbjct: 361 QKGKKHQQAAPLFDKVVFDKVKKGLGGNVRIILSGGAPLAAHIETFFRVVSCAHVLQGYG 420

Query: 421 LTETCGGTFVTLPNELPMLGTVGPPVPNVDVCLESVPEMGYDSHASTPRGEVCIKGDTLF 480
           LTETCGGTFVTLP+ELPMLGTVGPPVPNVD+CLESVPEMGYDSHASTPRGEVCIKGDTLF
Sbjct: 421 LTETCGGTFVTLPDELPMLGTVGPPVPNVDICLESVPEMGYDSHASTPRGEVCIKGDTLF 480

Query: 481 SGYYKRDDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY 540
           SGYYKRDDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY
Sbjct: 481 SGYYKRDDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY 540

Query: 541 GLVTDIEMIWIYGNSFESFLVAVVNPKKQSLEHWAEENGIKGDFNALCEDKRAKDYILGQ 600
           GLV+DIEMIWIYGNSFESFLVAVVNPKKQ+LE WAEENGIK DFNALCEDKRAKDYILGQ
Sbjct: 541 GLVSDIEMIWIYGNSFESFLVAVVNPKKQALEQWAEENGIKEDFNALCEDKRAKDYILGQ 600

Query: 601 LSKIAKEKKLKGYENIRAVHLDPLPFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGN 660
           LSKIAKE+KLKGYENIRAVHLDPLPFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGN
Sbjct: 601 LSKIAKEQKLKGYENIRAVHLDPLPFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGN 660

Query: 661 KPVA 665
           KP+A
Sbjct: 661 KPIA 664

BLAST of HG10009056 vs. ExPASy TrEMBL
Match: A0A5A7TH75 (Long chain acyl-CoA synthetase 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G001820 PE=4 SV=1)

HSP 1 Score: 1291.2 bits (3340), Expect = 0.0e+00
Identity = 630/689 (91.44%), Postives = 649/689 (94.19%), Query Frame = 0

Query: 1   MQRKYLVQVEEAKEAAAGRPSVGPVYRSIFAKDGFPPPVQGLETCWDIFRMSVEKNPGNR 60
           MQRKYLVQVEEAK+A AGRPSVGPVYRSIFAKDGFPPPVQGL+TCWDIFRMSVEKNPGNR
Sbjct: 1   MQRKYLVQVEEAKDATAGRPSVGPVYRSIFAKDGFPPPVQGLDTCWDIFRMSVEKNPGNR 60

Query: 61  MLGRRKIVEGNPAKAGECEWLTYKEVYELVLKIGNAMRGLGYGPGVKCGIYGANCPEWII 120
           MLGRRK+VEGNPAKAGE EWLTYKEVYELVLKIGNAMRGLGYGPG KCGIYGANCPEWII
Sbjct: 61  MLGRRKVVEGNPAKAGEYEWLTYKEVYELVLKIGNAMRGLGYGPGEKCGIYGANCPEWII 120

Query: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAEISISFVEEKKISELLKTLPNTTKFLKTV 180
           SMEACNAHGLYCVPLYDTLGAGAIEFII HAEISI+FVEEKKISELLKTLPNT KFLKT+
Sbjct: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIISHAEISIAFVEEKKISELLKTLPNTAKFLKTI 180

Query: 181 VSFGKVSDNHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPK 240
           VSFGKVSDNHKEEV KFGLEIY+WEEFLQKGDSQQ+DLPVK K DICTIMYTSGTTGDPK
Sbjct: 181 VSFGKVSDNHKEEVKKFGLEIYTWEEFLQKGDSQQFDLPVKEKSDICTIMYTSGTTGDPK 240

Query: 241 GVLLSNNAIIALIAGVKHLLTTVKEQVQENDVYISYLPLAHIFDRVIEEVFILNSASIGF 300
           GVLLSNNAIIALIAGVKHLLTTV E VQENDVYISYLPLAHIFDRVIEEVFILN ASIGF
Sbjct: 241 GVLLSNNAIIALIAGVKHLLTTVNENVQENDVYISYLPLAHIFDRVIEEVFILNGASIGF 300

Query: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGFLKKSLFNFAYSYKHNQM 360
           WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGFLK+SLFN AYSYK+NQM
Sbjct: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGFLKRSLFNLAYSYKYNQM 360

Query: 361 QKGKKHEEAAPLFDKVVFDKVKKGLGGKVRIILSGGAPLASHIETFFRVVSCAHVLQGY- 420
           QKGKKH++AAPLFDKVVFDKVKKGLGG VRIILSGGAPLA+HIETFFRVVSCAHVLQGY 
Sbjct: 361 QKGKKHQQAAPLFDKVVFDKVKKGLGGNVRIILSGGAPLAAHIETFFRVVSCAHVLQGYG 420

Query: 421 ------------------------GLTETCGGTFVTLPNELPMLGTVGPPVPNVDVCLES 480
                                   GLTETCGGTFVTLP+ELPMLGTVGPPVPNVD+CLES
Sbjct: 421 ISSLYLHFQKPKQREPKYHTFLFAGLTETCGGTFVTLPDELPMLGTVGPPVPNVDICLES 480

Query: 481 VPEMGYDSHASTPRGEVCIKGDTLFSGYYKRDDLTTEVLVDGWFHTGDVGEWQPDGSLKI 540
           VPEMGYDSHASTPRGEVCIKGDTLFSGYYKRDDLTTEVLVDGWFHTGDVGEWQPDGSLKI
Sbjct: 481 VPEMGYDSHASTPRGEVCIKGDTLFSGYYKRDDLTTEVLVDGWFHTGDVGEWQPDGSLKI 540

Query: 541 IDRKKNIFKLSQGEYVAVENLELIYGLVTDIEMIWIYGNSFESFLVAVVNPKKQSLEHWA 600
           IDRKKNIFKLSQGEYVAVENLELIYGLV+DIEMIWIYGNSFESFLVAVVNPKKQ+LE WA
Sbjct: 541 IDRKKNIFKLSQGEYVAVENLELIYGLVSDIEMIWIYGNSFESFLVAVVNPKKQALEQWA 600

Query: 601 EENGIKGDFNALCEDKRAKDYILGQLSKIAKEKKLKGYENIRAVHLDPLPFDIERDLLTP 660
           EENGIK DFNALCEDKRAKDYILGQLSKIAKE+KLKGYENIRAVHLDPLPFDIERDLLTP
Sbjct: 601 EENGIKEDFNALCEDKRAKDYILGQLSKIAKEQKLKGYENIRAVHLDPLPFDIERDLLTP 660

Query: 661 SFKKKRPQLLKYYQKEIDDMYKSGNKPVA 665
           SFKKKRPQLLKYYQK+IDDMYKSGNKP+A
Sbjct: 661 SFKKKRPQLLKYYQKKIDDMYKSGNKPIA 689

BLAST of HG10009056 vs. ExPASy TrEMBL
Match: A0A0A0KQR9 (AMP-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G150390 PE=4 SV=1)

HSP 1 Score: 1283.5 bits (3320), Expect = 0.0e+00
Identity = 619/650 (95.23%), Postives = 637/650 (98.00%), Query Frame = 0

Query: 1   MQRKYLVQVEEAKEAAAGRPSVGPVYRSIFAKDGFPPPVQGLETCWDIFRMSVEKNPGNR 60
           MQRKYLVQVEEAK+A AGRPSVGPVYRSIFAKDGFPPPVQGL+TCWDIFRMSVEKNPGNR
Sbjct: 1   MQRKYLVQVEEAKDATAGRPSVGPVYRSIFAKDGFPPPVQGLDTCWDIFRMSVEKNPGNR 60

Query: 61  MLGRRKIVEGNPAKAGECEWLTYKEVYELVLKIGNAMRGLGYGPGVKCGIYGANCPEWII 120
           MLGRRK+VEGNPAKAGE EWLTYKEVYELVLKIGNAMR LGYGPG KCGIYGANCPEWII
Sbjct: 61  MLGRRKVVEGNPAKAGEYEWLTYKEVYELVLKIGNAMRSLGYGPGEKCGIYGANCPEWII 120

Query: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAEISISFVEEKKISELLKTLPNTTKFLKTV 180
           SMEACNAHGLYCVPLYDTLGAGAIEFIICHAEI+I+FVEEKKISELLKTLPNT KFLKT+
Sbjct: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAEIAIAFVEEKKISELLKTLPNTAKFLKTI 180

Query: 181 VSFGKVSDNHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPK 240
           VSFGKVSDNHKEEVNKFGLEIY+WEEFLQKGDSQQYDLPVK K DICTIMYTSGTTGDPK
Sbjct: 181 VSFGKVSDNHKEEVNKFGLEIYTWEEFLQKGDSQQYDLPVKEKSDICTIMYTSGTTGDPK 240

Query: 241 GVLLSNNAIIALIAGVKHLLTTVKEQVQENDVYISYLPLAHIFDRVIEEVFILNSASIGF 300
           GVLLSN AIIALIAGVKHLLTTVKE VQENDVYISYLPLAHIFDRVIEEVFILN ASIGF
Sbjct: 241 GVLLSNTAIIALIAGVKHLLTTVKENVQENDVYISYLPLAHIFDRVIEEVFILNGASIGF 300

Query: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGFLKKSLFNFAYSYKHNQM 360
           WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGFLK+SLFNFAYSYK+NQM
Sbjct: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGFLKRSLFNFAYSYKYNQM 360

Query: 361 QKGKKHEEAAPLFDKVVFDKVKKGLGGKVRIILSGGAPLASHIETFFRVVSCAHVLQGYG 420
           QKGK H++AAPLFDKVVFDKVKKGLGG VRIILSGGAPLA+HIETFFRVVSCAHVLQGYG
Sbjct: 361 QKGKTHQQAAPLFDKVVFDKVKKGLGGNVRIILSGGAPLAAHIETFFRVVSCAHVLQGYG 420

Query: 421 LTETCGGTFVTLPNELPMLGTVGPPVPNVDVCLESVPEMGYDSHASTPRGEVCIKGDTLF 480
           LTETCGGTFV+LP+ELPMLGTVGPPVPNVD+CLESVPEMGYDSHASTPRGEVCIKGDTLF
Sbjct: 421 LTETCGGTFVSLPDELPMLGTVGPPVPNVDICLESVPEMGYDSHASTPRGEVCIKGDTLF 480

Query: 481 SGYYKRDDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY 540
           SGYYKRDDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY
Sbjct: 481 SGYYKRDDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY 540

Query: 541 GLVTDIEMIWIYGNSFESFLVAVVNPKKQSLEHWAEENGIKGDFNALCEDKRAKDYILGQ 600
           GLV+DIEMIWIYGNSFESFLVAVVNPKKQ+LE WAEENGIKGDFNALCEDKRAKDYILGQ
Sbjct: 541 GLVSDIEMIWIYGNSFESFLVAVVNPKKQALEQWAEENGIKGDFNALCEDKRAKDYILGQ 600

Query: 601 LSKIAKEKKLKGYENIRAVHLDPLPFDIERDLLTPSFKKKRPQLLKYYQK 651
           LSKIAKE+KLKGYENIRAVHLDPLPFDIERDLLTPSFKKKRPQLLKYYQK
Sbjct: 601 LSKIAKEQKLKGYENIRAVHLDPLPFDIERDLLTPSFKKKRPQLLKYYQK 650

BLAST of HG10009056 vs. ExPASy TrEMBL
Match: A0A6J1ER85 (long chain acyl-CoA synthetase 4-like OS=Cucurbita moschata OX=3662 GN=LOC111435809 PE=4 SV=1)

HSP 1 Score: 1280.4 bits (3312), Expect = 0.0e+00
Identity = 618/664 (93.07%), Postives = 642/664 (96.69%), Query Frame = 0

Query: 1   MQRKYLVQVEEAKEAAAGRPSVGPVYRSIFAKDGFPPPVQGLETCWDIFRMSVEKNPGNR 60
           MQRKYL QVEE KEAAAGRPSVGPVYRS+FAKDGFPP V+GLETCWDIFRMSVEKNPGNR
Sbjct: 1   MQRKYLFQVEEGKEAAAGRPSVGPVYRSVFAKDGFPPSVEGLETCWDIFRMSVEKNPGNR 60

Query: 61  MLGRRKIVEGNPAKAGECEWLTYKEVYELVLKIGNAMRGLGYGPGVKCGIYGANCPEWII 120
           MLGRRKIVEGNPAKAGE EWLTY++VYELVLKIGNAMR LGYGPG KCGIYGANCPEWII
Sbjct: 61  MLGRRKIVEGNPAKAGEYEWLTYQQVYELVLKIGNAMRSLGYGPGEKCGIYGANCPEWII 120

Query: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAEISISFVEEKKISELLKTLPNTTKFLKTV 180
           SMEACNAHGLYCVPLYDTLGAGAIEFIICHAE SI+FVEEKKI ELLKTLPNT   LKT+
Sbjct: 121 SMEACNAHGLYCVPLYDTLGAGAIEFIICHAETSIAFVEEKKIPELLKTLPNTANVLKTL 180

Query: 181 VSFGKVSDNHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPK 240
           VSFGKVS+NHKEEVNKFGLEIYSWEEFLQKGDSQQYD PVKGKGDICTIMYTSGTTGDPK
Sbjct: 181 VSFGKVSNNHKEEVNKFGLEIYSWEEFLQKGDSQQYDPPVKGKGDICTIMYTSGTTGDPK 240

Query: 241 GVLLSNNAIIALIAGVKHLLTTVKEQVQENDVYISYLPLAHIFDRVIEEVFILNSASIGF 300
           GVLLSN AIIALIAGVKHLL TVKE+VQENDVYISYLPLAHIFDRVIEEVFILN ASIGF
Sbjct: 241 GVLLSNKAIIALIAGVKHLLATVKEEVQENDVYISYLPLAHIFDRVIEEVFILNGASIGF 300

Query: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGFLKKSLFNFAYSYKHNQM 360
           WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGG LK+SLFNFAYSYK+NQM
Sbjct: 301 WRGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGLLKRSLFNFAYSYKYNQM 360

Query: 361 QKGKKHEEAAPLFDKVVFDKVKKGLGGKVRIILSGGAPLASHIETFFRVVSCAHVLQGYG 420
           QKGKKHE+AA LFDK+VFDKVKKGLGGKVRIILSG APLA+HIETFFRVVSCAHVLQGYG
Sbjct: 361 QKGKKHEQAATLFDKLVFDKVKKGLGGKVRIILSGAAPLAAHIETFFRVVSCAHVLQGYG 420

Query: 421 LTETCGGTFVTLPNELPMLGTVGPPVPNVDVCLESVPEMGYDSHASTPRGEVCIKGDTLF 480
           LTETCGGTFVTLPNEL MLGTVGPPVPNVD+CLESVPEMGYDS ASTPRGEVCIKGDTLF
Sbjct: 421 LTETCGGTFVTLPNELRMLGTVGPPVPNVDICLESVPEMGYDSLASTPRGEVCIKGDTLF 480

Query: 481 SGYYKRDDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY 540
           +GYYKR+DLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY
Sbjct: 481 AGYYKREDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIY 540

Query: 541 GLVTDIEMIWIYGNSFESFLVAVVNPKKQSLEHWAEENGIKGDFNALCEDKRAKDYILGQ 600
           GLV+DIEMIWIYGNSFE+FLVAVVNP++Q+LEHWAEENGIKGDFNALCEDKRAKDYILGQ
Sbjct: 541 GLVSDIEMIWIYGNSFEAFLVAVVNPQRQALEHWAEENGIKGDFNALCEDKRAKDYILGQ 600

Query: 601 LSKIAKEKKLKGYENIRAVHLDPLPFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGN 660
           LSKIAKEKKLKGYE I+AVHLDPLPFDIERDLLTPSFKKKRPQ+LKYYQKEID+MYK GN
Sbjct: 601 LSKIAKEKKLKGYEVIKAVHLDPLPFDIERDLLTPSFKKKRPQMLKYYQKEIDEMYKGGN 660

Query: 661 KPVA 665
           KPVA
Sbjct: 661 KPVA 664

BLAST of HG10009056 vs. TAIR 10
Match: AT4G23850.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 1008.8 bits (2607), Expect = 2.0e-294
Identity = 468/663 (70.59%), Postives = 565/663 (85.22%), Query Frame = 0

Query: 2   QRKYLVQVEEAKEAAAGRPSVGPVYRSIFAKDGFPPPVQGLETCWDIFRMSVEKNPGNRM 61
           Q+KY+ QVEE KE + GRPSVGPVYRSIFAKDGFP P++G+++CWD+FRMSVEK P N M
Sbjct: 4   QKKYIFQVEEGKEGSDGRPSVGPVYRSIFAKDGFPDPIEGMDSCWDVFRMSVEKYPNNPM 63

Query: 62  LGRRKIVEGNPAKAGECEWLTYKEVYELVLKIGNAMRGLGYGPGVKCGIYGANCPEWIIS 121
           LGRR+IV+G P   G+  W TY+EVY++V+K+GN++R +G     KCGIYGAN PEWIIS
Sbjct: 64  LGRREIVDGKP---GKYVWQTYQEVYDIVMKLGNSLRSVGVKDEAKCGIYGANSPEWIIS 123

Query: 122 MEACNAHGLYCVPLYDTLGAGAIEFIICHAEISISFVEEKKISELLKTLPNTTKFLKTVV 181
           MEACNAHGLYCVPLYDTLGA A+EFII H+E+SI FVEEKKISEL KT PN+T+++KTVV
Sbjct: 124 MEACNAHGLYCVPLYDTLGADAVEFIISHSEVSIVFVEEKKISELFKTCPNSTEYMKTVV 183

Query: 182 SFGKVSDNHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPKG 241
           SFG VS   KEE   FGL IY+W+EFL+ G+ +QYDLP+K K DICTIMYTSGTTGDPKG
Sbjct: 184 SFGGVSREQKEEAETFGLVIYAWDEFLKLGEGKQYDLPIKKKSDICTIMYTSGTTGDPKG 243

Query: 242 VLLSNNAIIALIAGVKHLLTTVKEQVQENDVYISYLPLAHIFDRVIEEVFILNSASIGFW 301
           V++SN +I+ LIAGV  LL +  E +   DVY+SYLPLAHIFDRVIEE FI + A+IGFW
Sbjct: 244 VMISNESIVTLIAGVIRLLKSANEALTVKDVYLSYLPLAHIFDRVIEECFIQHGAAIGFW 303

Query: 302 RGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGFLKKSLFNFAYSYKHNQMQ 361
           RGDVKLL+ED+ ELKPTIFCAVPRVL+R+Y GL +K+S GGFLKK +F+ A+SYK   M+
Sbjct: 304 RGDVKLLIEDLAELKPTIFCAVPRVLDRVYSGLQKKLSDGGFLKKFIFDSAFSYKFGYMK 363

Query: 362 KGKKHEEAAPLFDKVVFDKVKKGLGGKVRIILSGGAPLASHIETFFRVVSCAHVLQGYGL 421
           KG+ H EA+PLFDK+VF KVK+GLGG VRIILSG APLASH+E+F RVV+C HVLQGYGL
Sbjct: 364 KGQSHVEASPLFDKLVFSKVKQGLGGNVRIILSGAAPLASHVESFLRVVACCHVLQGYGL 423

Query: 422 TETCGGTFVTLPNELPMLGTVGPPVPNVDVCLESVPEMGYDSHASTPRGEVCIKGDTLFS 481
           TE+C GTFV+LP+EL MLGTVGPPVPNVD+ LESVPEM YD+ AST RGE+CI+G TLFS
Sbjct: 424 TESCAGTFVSLPDELGMLGTVGPPVPNVDIRLESVPEMEYDALASTARGEICIRGKTLFS 483

Query: 482 GYYKRDDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIYG 541
           GYYKR+DLT EVL+DGW HTGDVGEWQPDGS+KIIDRKKNIFKLSQGEYVAVEN+E IYG
Sbjct: 484 GYYKREDLTKEVLIDGWLHTGDVGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIYG 543

Query: 542 LVTDIEMIWIYGNSFESFLVAVVNPKKQSLEHWAEENGIKGDFNALCEDKRAKDYILGQL 601
            V  ++ +W+YGNSFESFL+A+ NP +  LE WA ENG+ GD++ALC++++AK++ILG+L
Sbjct: 544 EVQAVDSVWVYGNSFESFLIAIANPNQHILERWAAENGVSGDYDALCQNEKAKEFILGEL 603

Query: 602 SKIAKEKKLKGYENIRAVHLDPLPFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGNK 661
            K+AKEKK+KG+E I+A+HLDP+PFD+ERDLLTP+FKKKRPQLLKYYQ  ID+MYK+ N 
Sbjct: 604 VKMAKEKKMKGFEIIKAIHLDPVPFDMERDLLTPTFKKKRPQLLKYYQSVIDEMYKTINA 663

Query: 662 PVA 665
             A
Sbjct: 664 KFA 663

BLAST of HG10009056 vs. TAIR 10
Match: AT1G64400.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 983.4 bits (2541), Expect = 8.9e-287
Identity = 454/659 (68.89%), Postives = 557/659 (84.52%), Query Frame = 0

Query: 4   KYLVQVEEAKEAA-AGRPSVGPVYRSIFAKDGFPPPVQGLETCWDIFRMSVEKNPGNRML 63
           +Y+V+VE+ K+    G PSVGPVYRSI+AKDGFP P   L + WDIFR+SVEK+P N ML
Sbjct: 5   RYIVEVEKGKQGVDGGSPSVGPVYRSIYAKDGFPEPPDDLVSAWDIFRLSVEKSPNNPML 64

Query: 64  GRRKIVEGNPAKAGECEWLTYKEVYELVLKIGNAMRGLGYGPGVKCGIYGANCPEWIISM 123
           GRR+IV+G   KAG+  W TYKEV+ +V+K+GN++R +G G G KCGIYGAN PEWIISM
Sbjct: 65  GRREIVDG---KAGKYVWQTYKEVHNVVIKLGNSIRTIGVGKGDKCGIYGANSPEWIISM 124

Query: 124 EACNAHGLYCVPLYDTLGAGAIEFIICHAEISISFVEEKKISELLKTLPNTTKFLKTVVS 183
           EACNAHGLYCVPLYDTLGAGAIEFIICHAE+S++F EE KISELLKT P +TK+LK +VS
Sbjct: 125 EACNAHGLYCVPLYDTLGAGAIEFIICHAEVSLAFAEENKISELLKTAPKSTKYLKYIVS 184

Query: 184 FGKVSDNHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPKGV 243
           FG+V++N + E  +  L IYSW++FL+ G+ + Y+LP K + D+CTIMYTSGTTGDPKGV
Sbjct: 185 FGEVTNNQRVEAERHRLTIYSWDQFLKLGEGKHYELPEKRRSDVCTIMYTSGTTGDPKGV 244

Query: 244 LLSNNAIIALIAGVKHLLTTVKEQVQENDVYISYLPLAHIFDRVIEEVFILNSASIGFWR 303
           LL+N +II L+ GVK LL T+ E++   DVY+SYLPLAHIFDRVIEE+ I  +ASIGFWR
Sbjct: 245 LLTNESIIHLLEGVKKLLKTIDEELTSKDVYLSYLPLAHIFDRVIEELCIYEAASIGFWR 304

Query: 304 GDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGFLKKSLFNFAYSYKHNQMQK 363
           GDVK+L+EDI  LKPT+FCAVPRVLERIY GL QK+S GGF+KK LFNFA+ YKH  M+K
Sbjct: 305 GDVKILIEDIAALKPTVFCAVPRVLERIYTGLQQKLSDGGFVKKKLFNFAFKYKHKNMEK 364

Query: 364 GKKHEEAAPLFDKVVFDKVKKGLGGKVRIILSGGAPLASHIETFFRVVSCAHVLQGYGLT 423
           G+ HE+A+P+ DK+VF KVK+GLGG VR+ILSG APLA+HIE+F RVV+CAHVLQGYGLT
Sbjct: 365 GQPHEQASPIADKIVFKKVKEGLGGNVRLILSGAAPLAAHIESFLRVVACAHVLQGYGLT 424

Query: 424 ETCGGTFVTLPNELPMLGTVGPPVPNVDVCLESVPEMGYDSHASTPRGEVCIKGDTLFSG 483
           E+CGGTFV++PNEL MLGTVGPPVPNVD+ LESVPEMGYD+ AS PRGE+CI+G TLFSG
Sbjct: 425 ESCGGTFVSIPNELSMLGTVGPPVPNVDIRLESVPEMGYDALASNPRGEICIRGKTLFSG 484

Query: 484 YYKRDDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIYGL 543
           YYKR+DLT EV +DGW HTGDVGEWQPDG++KIIDRKKNIFKLSQGEYVAVENLE IY  
Sbjct: 485 YYKREDLTQEVFIDGWLHTGDVGEWQPDGAMKIIDRKKNIFKLSQGEYVAVENLENIYSH 544

Query: 544 VTDIEMIWIYGNSFESFLVAVVNPKKQSLEHWAEENGIKGDFNALCEDKRAKDYILGQLS 603
           V  IE IW+YGNS+ES+LVAVV P K  +EHWA+E+ + GDF ++C +++ K+++LG+ +
Sbjct: 545 VAAIESIWVYGNSYESYLVAVVCPSKIQIEHWAKEHKVSGDFESICRNQKTKEFVLGEFN 604

Query: 604 KIAKEKKLKGYENIRAVHLDPLPFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGNK 662
           ++AK+KKLKG+E I+ VHLD +PFD+ERDL+TPS+K KRPQLLKYYQKEID+MYK   +
Sbjct: 605 RVAKDKKLKGFELIKGVHLDTVPFDMERDLITPSYKMKRPQLLKYYQKEIDEMYKKNRE 660

BLAST of HG10009056 vs. TAIR 10
Match: AT4G11030.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 966.8 bits (2498), Expect = 8.6e-282
Identity = 450/663 (67.87%), Postives = 543/663 (81.90%), Query Frame = 0

Query: 2   QRKYLVQVEEAKEAAAGRPSVGPVYRSIFAKDGFPPPVQGLETCWDIFRMSVEKNPGNRM 61
           Q++++ +VE AKEA  G PSVGPVYRS FA++GFP P+ G+++CWDIFR +VEK P NRM
Sbjct: 4   QKRFIFEVEAAKEATDGNPSVGPVYRSTFAQNGFPNPIDGIQSCWDIFRTAVEKYPNNRM 63

Query: 62  LGRRKIVEGNPAKAGECEWLTYKEVYELVLKIGNAMRGLGYGPGVKCGIYGANCPEWIIS 121
           LGRR+I  G   KAG+  W TYKEVY++V+K+GN++R  G   G KCGIYG NC EWIIS
Sbjct: 64  LGRREISNG---KAGKYVWKTYKEVYDIVIKLGNSLRSCGIKEGEKCGIYGINCCEWIIS 123

Query: 122 MEACNAHGLYCVPLYDTLGAGAIEFIICHAEISISFVEEKKISELLKTLPNTTKFLKTVV 181
           MEACNAHGLYCVPLYDTLGAGA+EFII HAE+SI+FVEEKKI EL KT PN+TK++KTVV
Sbjct: 124 MEACNAHGLYCVPLYDTLGAGAVEFIISHAEVSIAFVEEKKIPELFKTCPNSTKYMKTVV 183

Query: 182 SFGKVSDNHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPKG 241
           SFG V    KEE  K GL I+SW+EFL+ G+ +QY+LP+K   DICTIMYTSGTTGDPKG
Sbjct: 184 SFGGVKPEQKEEAEKLGLVIHSWDEFLKLGEGKQYELPIKKPSDICTIMYTSGTTGDPKG 243

Query: 242 VLLSNNAIIALIAGVKHLLTTVKEQVQENDVYISYLPLAHIFDRVIEEVFILNSASIGFW 301
           V++SN +I+ +  GV H L  V   + E DVYISYLPLAH+FDR IEE  I    SIGFW
Sbjct: 244 VMISNESIVTITTGVMHFLGNVNASLSEKDVYISYLPLAHVFDRAIEECIIQVGGSIGFW 303

Query: 302 RGDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGFLKKSLFNFAYSYKHNQMQ 361
           RGDVKLL+ED+GELKP+IFCAVPRVL+R+Y GL QK+S GGF KK +F+ A+SYK   M+
Sbjct: 304 RGDVKLLIEDLGELKPSIFCAVPRVLDRVYTGLQQKLSGGGFFKKKVFDVAFSYKFGNMK 363

Query: 362 KGKKHEEAAPLFDKVVFDKVKKGLGGKVRIILSGGAPLASHIETFFRVVSCAHVLQGYGL 421
           KG+ H  A+P  DK+VF+KVK+GLGG VRIILSG APLASHIE+F RVV+C +VLQGYGL
Sbjct: 364 KGQSHVAASPFCDKLVFNKVKQGLGGNVRIILSGAAPLASHIESFLRVVACCNVLQGYGL 423

Query: 422 TETCGGTFVTLPNELPMLGTVGPPVPNVDVCLESVPEMGYDSHASTPRGEVCIKGDTLFS 481
           TE+C GTF T P+EL MLGTVGPPVPNVD+ LESVPEM YD+  STPRGE+CI+G TLFS
Sbjct: 424 TESCAGTFATFPDELDMLGTVGPPVPNVDIRLESVPEMNYDALGSTPRGEICIRGKTLFS 483

Query: 482 GYYKRDDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIYG 541
           GYYKR+DLT EV +DGW HTGDVGEWQP+GS+KIIDRKKNIFKL+QGEYVAVENLE +Y 
Sbjct: 484 GYYKREDLTKEVFIDGWLHTGDVGEWQPNGSMKIIDRKKNIFKLAQGEYVAVENLENVYS 543

Query: 542 LVTDIEMIWIYGNSFESFLVAVVNPKKQSLEHWAEENGIKGDFNALCEDKRAKDYILGQL 601
            V  IE IW+YGNSFESFLVA+ NP +Q+LE WA ENG+ GDFN++C++ +AK +ILG+L
Sbjct: 544 QVEVIESIWVYGNSFESFLVAIANPAQQTLERWAVENGVNGDFNSICQNAKAKAFILGEL 603

Query: 602 SKIAKEKKLKGYENIRAVHLDPLPFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGNK 661
            K AKE KLKG+E I+ VHL+P+ FD+ERDLLTP++KKKRPQLLKYYQ  I +MYK+  +
Sbjct: 604 VKTAKENKLKGFEIIKDVHLEPVAFDMERDLLTPTYKKKRPQLLKYYQNVIHEMYKTTKE 663

Query: 662 PVA 665
            +A
Sbjct: 664 SLA 663

BLAST of HG10009056 vs. TAIR 10
Match: AT1G49430.1 (long-chain acyl-CoA synthetase 2 )

HSP 1 Score: 734.6 bits (1895), Expect = 7.2e-212
Identity = 341/658 (51.82%), Postives = 470/658 (71.43%), Query Frame = 0

Query: 9   VEEAKEAAAGRPSVGPVYRSIFAKDGFPPPVQGLETCWDIFRMSVEKNPGNRMLGRRKIV 68
           VEE + A A  PS GPVYR  +AKDG       +++ W  F  +V+K P  +MLG+R   
Sbjct: 11  VEEGRPATAEHPSAGPVYRCKYAKDGLLDLPTDIDSPWQFFSEAVKKYPNEQMLGQRVTT 70

Query: 69  EGNPAKAGECEWLTYKEVYELVLKIGNAMRGLGYGPGVKCGIYGANCPEWIISMEACNAH 128
           +   +K G   W+TYKE ++  ++IG+A+R  G  PG  CGIYGANCPEWII+MEAC + 
Sbjct: 71  D---SKVGPYTWITYKEAHDAAIRIGSAIRSRGVDPGHCCGIYGANCPEWIIAMEACMSQ 130

Query: 129 GLYCVPLYDTLGAGAIEFIICHAEISISFVEEKKISELLKTLPNTTKFLKTVVSFGKVSD 188
           G+  VPLYD+LG  A+EFII HAE+S+ FV+EK +S +L      +  LKT+VSFG+VS 
Sbjct: 131 GITYVPLYDSLGVNAVEFIINHAEVSLVFVQEKTVSSILSCQKGCSSNLKTIVSFGEVSS 190

Query: 189 NHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPKGVLLSNNA 248
             KEE     + ++SW EF   G+  + +LP K K DICTIMYTSGTTG+PKGV+L+N A
Sbjct: 191 TQKEEAKNQCVSLFSWNEFSLMGNLDEANLPRKRKTDICTIMYTSGTTGEPKGVILNNAA 250

Query: 249 IIALIAGVKHLLTTVKEQVQENDVYISYLPLAHIFDRVIEEVFILNSASIGFWRGDVKLL 308
           I   +  +  +L         +DV+ SYLPLAH +D+V+E  F+   +S+G+WRGD++ L
Sbjct: 251 ISVQVLSIDKMLEVTDRSCDTSDVFFSYLPLAHCYDQVMEIYFLSRGSSVGYWRGDIRYL 310

Query: 309 VEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGFLKKSLFNFAYSYKHNQMQKGKKHEE 368
           ++D+  LKPT+FC VPRV +++Y G+ QKIS+ G ++K LF+FAY+YK   M+KG   EE
Sbjct: 311 MDDVQALKPTVFCGVPRVYDKLYAGIMQKISASGLIRKKLFDFAYNYKLGNMRKGFSQEE 370

Query: 369 AAPLFDKVVFDKVKKGLGGKVRIILSGGAPLASHIETFFRVVSCAHVLQGYGLTETCGGT 428
           A+P  D+++FDK+K+ LGG+  ++LSG APL  H+E F R++  +++ QGYGLTE+CGG+
Sbjct: 371 ASPRLDRLMFDKIKEALGGRAHMLLSGAAPLPRHVEEFLRIIPASNLSQGYGLTESCGGS 430

Query: 429 FVTLPNELPMLGTVGPPVPNVDVCLESVPEMGYDS-HASTPRGEVCIKGDTLFSGYYKRD 488
           F TL     M+GTVG P+P V+  L SVPEMGYD+  A  PRGE+C++G+++FSGY+KR 
Sbjct: 431 FTTLAGVFSMVGTVGVPMPTVEARLVSVPEMGYDAFSADVPRGEICLRGNSMFSGYHKRQ 490

Query: 489 DLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIYGLVTDIE 548
           DLT +VL+DGWFHTGD+GEWQ DGS+KIIDRKKNIFKLSQGEYVAVENLE  Y     I 
Sbjct: 491 DLTDQVLIDGWFHTGDIGEWQEDGSMKIIDRKKNIFKLSQGEYVAVENLENTYSRCPLIA 550

Query: 549 MIWIYGNSFESFLVAVVNPKKQSLEHWAEEN-GIKGDFNALCEDKRAKDYILGQLSKIAK 608
            IW+YGNSFESFLV VV P ++++E WA+ N     DF +LC++ +A+ Y L +L+  AK
Sbjct: 551 QIWVYGNSFESFLVGVVVPDRKAIEDWAKLNYQSPNDFESLCQNLKAQKYFLDELNSTAK 610

Query: 609 EKKLKGYENIRAVHLDPLPFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYKSGNKPVA 665
           + +LKG+E ++A+HL+P PFDIERDL+TP+FK KRPQLL++Y+  +D +Y    + +A
Sbjct: 611 QYQLKGFEMLKAIHLEPNPFDIERDLITPTFKLKRPQLLQHYKGIVDQLYSEAKRSMA 665

BLAST of HG10009056 vs. TAIR 10
Match: AT2G47240.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 661.8 bits (1706), Expect = 5.9e-190
Identity = 318/655 (48.55%), Postives = 444/655 (67.79%), Query Frame = 0

Query: 3   RKYLVQVEEAKEAAAGRPSVGPVYRSIFAKDGFPPPVQGLETCWDIFRMSVEKNPGNRML 62
           + +  +VEE  +   G+PSVGPVYR++ ++ GFPP    + T WDIF  SVEK P N ML
Sbjct: 2   KSFAAKVEEGVKGIDGKPSVGPVYRNLLSEKGFPPIDSEITTAWDIFSKSVEKFPDNNML 61

Query: 63  GRRKIVEGNPAKAGECEWLTYKEVYELVLKIGNAMRGLGYGPGVKCGIYGANCPEWIISM 122
           G R+IV+    K G   W TYKEVYE VL+IG+A+R  G  PG + GIYG NCP+WII+M
Sbjct: 62  GWRRIVD---EKVGPYMWKTYKEVYEEVLQIGSALRAAGAEPGSRVGIYGVNCPQWIIAM 121

Query: 123 EACNAHGLYCVPLYDTLGAGAIEFIICHAEISISFVEEKKISELLKTLPNTTKFLKTVVS 182
           EAC AH L CVPLYDTLG+GA+++I+ HAEI   FV++ KI  LL+      K LK +VS
Sbjct: 122 EACAAHTLICVPLYDTLGSGAVDYIVEHAEIDFVFVQDTKIKGLLEPDCKCAKRLKAIVS 181

Query: 183 FGKVSDNHKEEVNKFGLEIYSWEEFLQKGDSQQYDLPVKGKGDICTIMYTSGTTGDPKGV 242
           F  VSD    + ++ G++ YSW +FL  G  +  D       +ICTIMYTSGT+GDPKGV
Sbjct: 182 FTNVSDELSHKASEIGVKTYSWIDFLHMGREKPEDTNPPKAFNICTIMYTSGTSGDPKGV 241

Query: 243 LLSNNAIIALIAGVKHLLTTVKEQVQENDVYISYLPLAHIFDRVIEEVFILNSASIGFWR 302
           +L++ A+   + G+   +   ++++  +DVY+S+LPLAHI DR+ EE F    AS+G++ 
Sbjct: 242 VLTHQAVATFVVGMDLYMDQFEDKMTHDDVYLSFLPLAHILDRMNEEYFFRKGASVGYYH 301

Query: 303 GDVKLLVEDIGELKPTIFCAVPRVLERIYGGLNQKISSGGFLKKSLFNFAYSYKHNQMQK 362
           G++ +L +DI ELKPT    VPRV ERI+ G+ + +      ++ +FN  Y +K   + +
Sbjct: 302 GNLNVLRDDIQELKPTYLAGVPRVFERIHEGIQKALQELNPRRRFIFNALYKHKLAWLNR 361

Query: 363 GKKHEEAAPLFDKVVFDKVKKGLGGKVRIILSGGAPLASHIETFFRVVSCAHVLQGYGLT 422
           G  H +A+P+ D + F K++  LGG++R+++SGGAPL+  IE F RV  C  V+QGYGLT
Sbjct: 362 GYSHSKASPMADFIAFRKIRDKLGGRIRLLVSGGAPLSPEIEEFLRVTCCCFVVQGYGLT 421

Query: 423 ETCGGTFVTLPNELPMLGTVGPPVPNVDVCLESVPEMGYDSHASTPRGEVCIKGDTLFSG 482
           ET GGT +  P+E+ MLGTVG P    ++ LE V EMGYD     P GE+CI+G  +FSG
Sbjct: 422 ETLGGTALGFPDEMCMLGTVGIPAVYNEIRLEEVSEMGYDPLGENPAGEICIRGQCMFSG 481

Query: 483 YYKRDDLTTEVLVDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLELIYGL 542
           YYK  +LT EV+ DGWFHTGD+GE  P+G LKIIDRKKN+ KLSQGEYVA+E+LE I+G 
Sbjct: 482 YYKNPELTEEVMKDGWFHTGDIGEILPNGVLKIIDRKKNLIKLSQGEYVALEHLENIFGQ 541

Query: 543 VTDIEMIWIYGNSFESFLVAVVNPKKQSLEHWAEENGIKGDFNALCEDKRAKDYILGQLS 602
            + ++ IW+YG+SF+S LVAVV P  +++  WA++ G    F  LC     K++I+ +L 
Sbjct: 542 NSVVQDIWVYGDSFKSMLVAVVVPNPETVNRWAKDLGFTKPFEELCSFPELKEHIISELK 601

Query: 603 KIAKEKKLKGYENIRAVHLDPLPFDIERDLLTPSFKKKRPQLLKYYQKEIDDMYK 658
             A++ KL+ +E I+AV ++  PFD+ERDL+T + K +R  LLKYYQ +ID+MY+
Sbjct: 602 STAEKNKLRKFEYIKAVTVETKPFDVERDLVTATLKNRRNNLLKYYQVQIDEMYR 653

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004143868.10.0e+0095.18long chain acyl-CoA synthetase 4 [Cucumis sativus][more]
XP_008437390.10.0e+0095.03PREDICTED: long chain acyl-CoA synthetase 4-like [Cucumis melo] >TYK06087.1 long... [more]
XP_038876249.10.0e+0095.48long chain acyl-CoA synthetase 4-like [Benincasa hispida][more]
KAA0042684.10.0e+0091.44long chain acyl-CoA synthetase 4-like [Cucumis melo var. makuwa][more]
XP_023549978.10.0e+0093.37long chain acyl-CoA synthetase 4-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9T0A02.8e-29370.59Long chain acyl-CoA synthetase 4 OS=Arabidopsis thaliana OX=3702 GN=LACS4 PE=2 S... [more]
Q9C7W41.3e-28568.89Long chain acyl-CoA synthetase 3 OS=Arabidopsis thaliana OX=3702 GN=LACS3 PE=2 S... [more]
Q9T0091.2e-28067.87Long chain acyl-CoA synthetase 5 OS=Arabidopsis thaliana OX=3702 GN=LACS5 PE=2 S... [more]
M4ISH11.0e-22354.49Probable CoA ligase CCL6 OS=Humulus lupulus OX=3486 GN=CCL6 PE=2 SV=1[more]
Q9XIA91.0e-21051.82Long chain acyl-CoA synthetase 2 OS=Arabidopsis thaliana OX=3702 GN=LACS2 PE=2 S... [more]
Match NameE-valueIdentityDescription
A0A5D3C2P40.0e+0095.03Long chain acyl-CoA synthetase 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3AUH90.0e+0095.03long chain acyl-CoA synthetase 4-like OS=Cucumis melo OX=3656 GN=LOC103482821 PE... [more]
A0A5A7TH750.0e+0091.44Long chain acyl-CoA synthetase 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A0A0KQR90.0e+0095.23AMP-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G150390... [more]
A0A6J1ER850.0e+0093.07long chain acyl-CoA synthetase 4-like OS=Cucurbita moschata OX=3662 GN=LOC111435... [more]
Match NameE-valueIdentityDescription
AT4G23850.12.0e-29470.59AMP-dependent synthetase and ligase family protein [more]
AT1G64400.18.9e-28768.89AMP-dependent synthetase and ligase family protein [more]
AT4G11030.18.6e-28267.87AMP-dependent synthetase and ligase family protein [more]
AT1G49430.17.2e-21251.82long-chain acyl-CoA synthetase 2 [more]
AT2G47240.15.9e-19048.55AMP-dependent synthetase and ligase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 69..526
e-value: 1.9E-100
score: 336.4
IPR042099ANL, N-terminal domainGENE3D3.40.50.12780coord: 40..518
e-value: 2.6E-86
score: 291.8
NoneNo IPR availablePANTHERPTHR43272:SF81LONG CHAIN ACYL-COA SYNTHETASE 4-LIKEcoord: 4..659
NoneNo IPR availablePANTHERPTHR43272LONG-CHAIN-FATTY-ACID--COA LIGASEcoord: 4..659
NoneNo IPR availableCDDcd05927LC-FACS_eukcoord: 76..657
e-value: 0.0
score: 785.638
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 42..657
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 229..240

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10009056.1HG10009056.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0001676 long-chain fatty acid metabolic process
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016020 membrane
molecular_function GO:0004467 long-chain fatty acid-CoA ligase activity