HG10008951 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10008951
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionPectin lyase-like superfamily protein
LocationChr06: 1009043 .. 1012035 (-)
RNA-Seq ExpressionHG10008951
SyntenyHG10008951
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATCATTCTCCAAAGCCCACAAAAACCCATGTAATTTTTTTTTTATTTTTCTTAATTTCTCTTTGAATTTCAATTTCGTTCTCTGTTTTTTCTTTCAATTAATTAAATAGGTTTTGCTTGTTCTTCTTCATTTCATTGATTTTTTCATTTTGATTATTGAAAATGATTAGTGGGTTTCTGTTCATATACCCAATTTGTTCTATCGAAAATGTAACCGATAAAAAGATGATTTCATAAACCCAACAACCGTTTTTTCCTAGTAATTTGTTCATCAATTTCCTCGAGAAACAAACAGAAATTGAATCATCAGTTATTTTGTACGGCAGGTTACGGCGGCGAGAATCTCGGCCGTGATTGTACTGGCATTGGTGAGTTTAGCCACCGTGGAGGGCAAAAAGCCTTGTCCGACTACAAATTACTTAGAAGTTCCGGCGATCAGTTGCCGGAAACACACGGCGTTGCTGACGGACTTCGGCGGCGTTGGCGACGGAGTAACTTCAAATACTCATGCATTCCGACAAGCGATTGAACACCTCAGCCCACTGGCGGCAGACGGCGGTGCTCAGCTAATTGTGCCGCCGGGAAAATGGCTGACCGGAAGTTTTAATCTGACCAGTCATTTCACACTTTTTGTCCATAAAGATGCAAAAATTCTTGCTTCCCAGGTTTGACCTTAAACGGAATTGCTAATTTTTATTTGTATATATGGAAATGTAATCTGTCACATGTGGGATTTTTTAAAACAAAATTAATTTTTTTTTTGTAATTATGGTTTTTATTTTCTTATTTTTAGTATTTTGATATGCATTGACCAAGAATTATGTCTTTTTGGTATTAAATTATTACAATTTTTTTAGTTTTAACAATATTTAAAACGGTAATTTGAATTTTTGACTTTCACAAATTACACCCCGTATTAAAAACACATGGATATTAGTTTATAATTTTGATTAGAAGGTGATTTTGGAACAACTAAAAAGTGGAAAAACATATATGAATTTTTTAAAATTAATCAATGATAAGGAATTTCATTTAGTGTAGAACAAAAAAGATGACCTTGTAGTCTTCGTTTTTTAGTGTAATTTTCAAATAATAATTTTGGTATTCATCATTATTTATTTTAAAAGTAACATTCATTGTTTTTCCGAAATGGAAATTAATAATAATAATAATTGGGGTAGGTATTTATTGACTTCATTGTTGGTGTCAAATTGAATGTATCTAAATTGAGTTTTAATTTTTGAAAAAAAAAGTATAATTTAAAACGTATATTAAGGTAGTGATTCGGTGTGGGTGAGAGTATTGAGAACTGATGGAGTTTCATAAACCAACGGTGCAGGATGAATCAGAATGGCCACTTGTCGCAGTTCTCCCATCTTATGGGAGGGGAAGAGATGCTCCTGGTGGACGATACAGCAGCCTCATCTTTGGAACCAATCTCACTGATGTTGTCATCACAGGTGATTATTTTCTCTGCCTTTTGTTTTCTAATTTTATAAAATTAATTTTTGACTTTTTTGCAAAAGAAGAAATGGGAAATTAAATTGAACTTCCTTCAATTATTCATTTCCTAAATTTATTTTTCAAAATTTTCCTTAAATCGTTAATGTTTATTATAGACGTTTAAATGGTTTATAAGAAAATAAATAAAATTAGAAACGGTTAAAATAAAATTGTATTATATTTGGAAAGAAATAATAAAAATTTGTGAGACGTGGCACAGGTAATAATGGTACAATTGACGGGCAAGGATCTTATTGGTGGGATAAGTTCCATAAAAACGAGCTCAACGTAACACGACCGTACCTGATCGAGATCATATACTCAGATCAAATCCAGATCTCTGATCTCACTTTGGTCAACTCTCCGTCTTGGTTTGTCCATCCGATCTACAGCAGGTTTGACTATTTACCTTCATCACACACGTTTCATTCCCTCTCCTCATCCGCACTAGATCCCAACTCATTCATACACAAAATTTTAAGCCTTAGTTTATCTTTGGGTTAATTTTCAAGTAATTAACTTTTATTATTTTAAATAAAACAATTTTATAATATAACATTTTAAATTGGAAAGTAATTTAAAATAGCATAATGATAATTTTCGTTTGTTGAAAAATGCAGAAATGTAATTATCCAAGGGCTCACCATTCTGGCTCCGATCGACTCTCCCAACACCGACGGAATCGATCCAGGTTTCCTTCAAAACCTTCAAATCCTTCTTTCAAATAGATATTTTCTTCTTCATCATTTCTTCTCCCTAATTCTTCTTTCAATTTCACAGATTCTTGCTCCAATACTCGAATCGAAGACTGCTTCATCGTCTCCGGCGATGACTGTATCGCCGTCAAGAGCGGATGGGACCAATACGGTATCAAATTCGGAATGCCGACTGAAGACCTCGTTATTCGCCGACTCACCTGCATCTCACCGGATTCCGCCGGCATTGCCCTCGGCAGCGAGATGTCCGGCGGTATCCGCAACGTTAGAATCGAAAACGTCACTGGAATCAACACGCAATCCGCCGTGAGAATCAAAACCGCTCGCGGACGAGGCGGTTTCGTGAAGGACATTTTCGTCCGAGGAATGTATCTATCGACGATGAAATACGTATTCTGGATGACTGGTAACTACAAATCGCATCCGGATGAGAAGTTCGATCCCGCGGCGGTCCCGGAGATTACGAACATTAATTACAGAGACGTGGTGGCGGAGAATGTTACTTACTCGGCGAGATTGGAAGGGATTTCGGGAGATCCTTTTACGGATATTTGTATTTCGAATGTGAAGATTGGACTGACGGCGAAACCTAAAAAGCTGCAGTGGAACTGTACGGACGTTGAGGGATTCAGTAGCGATGTGGTGCCGCCGCCATGTGCTCCGTTGGCGAAGGCGGCGAAAGGCGGCGGATGTTACTTTCCTGAGGATCAGCTGCCGATTGAGAAGGTTCAGTTGAAGACTTGCTTTGTTAAAAATGCTGCCTTCAAGTGA

mRNA sequence

ATGGATCATTCTCCAAAGCCCACAAAAACCCATGTTACGGCGGCGAGAATCTCGGCCGTGATTGTACTGGCATTGGTGAGTTTAGCCACCGTGGAGGGCAAAAAGCCTTGTCCGACTACAAATTACTTAGAAGTTCCGGCGATCAGTTGCCGGAAACACACGGCGTTGCTGACGGACTTCGGCGGCGTTGGCGACGGAGTAACTTCAAATACTCATGCATTCCGACAAGCGATTGAACACCTCAGCCCACTGGCGGCAGACGGCGGTGCTCAGCTAATTGTGCCGCCGGGAAAATGGCTGACCGGAAGTTTTAATCTGACCAGTCATTTCACACTTTTTGTCCATAAAGATGCAAAAATTCTTGCTTCCCAGGATGAATCAGAATGGCCACTTGTCGCAGTTCTCCCATCTTATGGGAGGGGAAGAGATGCTCCTGGTGGACGATACAGCAGCCTCATCTTTGGAACCAATCTCACTGATGTTGTCATCACAGGTAATAATGGTACAATTGACGGGCAAGGATCTTATTGGTGGGATAAGTTCCATAAAAACGAGCTCAACGTAACACGACCGTACCTGATCGAGATCATATACTCAGATCAAATCCAGATCTCTGATCTCACTTTGGTCAACTCTCCGTCTTGGTTTGTCCATCCGATCTACAGCAGAAATGTAATTATCCAAGGGCTCACCATTCTGGCTCCGATCGACTCTCCCAACACCGACGGAATCGATCCAGATTCTTGCTCCAATACTCGAATCGAAGACTGCTTCATCGTCTCCGGCGATGACTGTATCGCCGTCAAGAGCGGATGGGACCAATACGGTATCAAATTCGGAATGCCGACTGAAGACCTCGTTATTCGCCGACTCACCTGCATCTCACCGGATTCCGCCGGCATTGCCCTCGGCAGCGAGATGTCCGGCGGTATCCGCAACGTTAGAATCGAAAACGTCACTGGAATCAACACGCAATCCGCCGTGAGAATCAAAACCGCTCGCGGACGAGGCGGTTTCGTGAAGGACATTTTCGTCCGAGGAATGTATCTATCGACGATGAAATACGTATTCTGGATGACTGGTAACTACAAATCGCATCCGGATGAGAAGTTCGATCCCGCGGCGGTCCCGGAGATTACGAACATTAATTACAGAGACGTGGTGGCGGAGAATGTTACTTACTCGGCGAGATTGGAAGGGATTTCGGGAGATCCTTTTACGGATATTTGTATTTCGAATGTGAAGATTGGACTGACGGCGAAACCTAAAAAGCTGCAGTGGAACTGTACGGACGTTGAGGGATTCAGTAGCGATGTGGTGCCGCCGCCATGTGCTCCGTTGGCGAAGGCGGCGAAAGGCGGCGGATGTTACTTTCCTGAGGATCAGCTGCCGATTGAGAAGGTTCAGTTGAAGACTTGCTTTGTTAAAAATGCTGCCTTCAAGTGA

Coding sequence (CDS)

ATGGATCATTCTCCAAAGCCCACAAAAACCCATGTTACGGCGGCGAGAATCTCGGCCGTGATTGTACTGGCATTGGTGAGTTTAGCCACCGTGGAGGGCAAAAAGCCTTGTCCGACTACAAATTACTTAGAAGTTCCGGCGATCAGTTGCCGGAAACACACGGCGTTGCTGACGGACTTCGGCGGCGTTGGCGACGGAGTAACTTCAAATACTCATGCATTCCGACAAGCGATTGAACACCTCAGCCCACTGGCGGCAGACGGCGGTGCTCAGCTAATTGTGCCGCCGGGAAAATGGCTGACCGGAAGTTTTAATCTGACCAGTCATTTCACACTTTTTGTCCATAAAGATGCAAAAATTCTTGCTTCCCAGGATGAATCAGAATGGCCACTTGTCGCAGTTCTCCCATCTTATGGGAGGGGAAGAGATGCTCCTGGTGGACGATACAGCAGCCTCATCTTTGGAACCAATCTCACTGATGTTGTCATCACAGGTAATAATGGTACAATTGACGGGCAAGGATCTTATTGGTGGGATAAGTTCCATAAAAACGAGCTCAACGTAACACGACCGTACCTGATCGAGATCATATACTCAGATCAAATCCAGATCTCTGATCTCACTTTGGTCAACTCTCCGTCTTGGTTTGTCCATCCGATCTACAGCAGAAATGTAATTATCCAAGGGCTCACCATTCTGGCTCCGATCGACTCTCCCAACACCGACGGAATCGATCCAGATTCTTGCTCCAATACTCGAATCGAAGACTGCTTCATCGTCTCCGGCGATGACTGTATCGCCGTCAAGAGCGGATGGGACCAATACGGTATCAAATTCGGAATGCCGACTGAAGACCTCGTTATTCGCCGACTCACCTGCATCTCACCGGATTCCGCCGGCATTGCCCTCGGCAGCGAGATGTCCGGCGGTATCCGCAACGTTAGAATCGAAAACGTCACTGGAATCAACACGCAATCCGCCGTGAGAATCAAAACCGCTCGCGGACGAGGCGGTTTCGTGAAGGACATTTTCGTCCGAGGAATGTATCTATCGACGATGAAATACGTATTCTGGATGACTGGTAACTACAAATCGCATCCGGATGAGAAGTTCGATCCCGCGGCGGTCCCGGAGATTACGAACATTAATTACAGAGACGTGGTGGCGGAGAATGTTACTTACTCGGCGAGATTGGAAGGGATTTCGGGAGATCCTTTTACGGATATTTGTATTTCGAATGTGAAGATTGGACTGACGGCGAAACCTAAAAAGCTGCAGTGGAACTGTACGGACGTTGAGGGATTCAGTAGCGATGTGGTGCCGCCGCCATGTGCTCCGTTGGCGAAGGCGGCGAAAGGCGGCGGATGTTACTTTCCTGAGGATCAGCTGCCGATTGAGAAGGTTCAGTTGAAGACTTGCTTTGTTAAAAATGCTGCCTTCAAGTGA

Protein sequence

MDHSPKPTKTHVTAARISAVIVLALVSLATVEGKKPCPTTNYLEVPAISCRKHTALLTDFGGVGDGVTSNTHAFRQAIEHLSPLAADGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAKILASQDESEWPLVAVLPSYGRGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGSYWWDKFHKNELNVTRPYLIEIIYSDQIQISDLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPNTDGIDPDSCSNTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIRRLTCISPDSAGIALGSEMSGGIRNVRIENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMTGNYKSHPDEKFDPAAVPEITNINYRDVVAENVTYSARLEGISGDPFTDICISNVKIGLTAKPKKLQWNCTDVEGFSSDVVPPPCAPLAKAAKGGGCYFPEDQLPIEKVQLKTCFVKNAAFK
Homology
BLAST of HG10008951 vs. NCBI nr
Match: XP_038906749.1 (probable polygalacturonase [Benincasa hispida])

HSP 1 Score: 953.4 bits (2463), Expect = 7.7e-274
Identity = 458/479 (95.62%), Postives = 468/479 (97.70%), Query Frame = 0

Query: 1   MDHSPKPTKTHVTAARISAVIVLALVSLATVEGKKPCPTTNYLEVPAISCRKHTALLTDF 60
           MD     TK HVTAARISAVI+LALVSLATVEGKKPCPTTNYLEVPAISCRKHTA+LTDF
Sbjct: 1   MDLPKNSTKFHVTAARISAVIILALVSLATVEGKKPCPTTNYLEVPAISCRKHTAVLTDF 60

Query: 61  GGVGDGVTSNTHAFRQAIEHLSPLAADGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAKI 120
           GGVGDGVTSNTHAFRQAIEHLSPLAADGGAQLIVPPGKWLTGSFNLTSHFTLFVHK A I
Sbjct: 61  GGVGDGVTSNTHAFRQAIEHLSPLAADGGAQLIVPPGKWLTGSFNLTSHFTLFVHKYATI 120

Query: 121 LASQDESEWPLVAVLPSYGRGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGSYWWDK 180
           LASQDESEWPLVAVLPSYGRGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGSYWWDK
Sbjct: 121 LASQDESEWPLVAVLPSYGRGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGSYWWDK 180

Query: 181 FHKNELNVTRPYLIEIIYSDQIQISDLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPN 240
           FHK+ELNVTRPYLIE++YSDQIQISDLTLVNSPSWFVHPIYSRNVII+GLTILAPIDSPN
Sbjct: 181 FHKDELNVTRPYLIEMMYSDQIQISDLTLVNSPSWFVHPIYSRNVIIRGLTILAPIDSPN 240

Query: 241 TDGIDPDSCSNTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIRRLTCISPDSAG 300
           TDGIDPDSCSNTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIRRLTCISPDSAG
Sbjct: 241 TDGIDPDSCSNTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIRRLTCISPDSAG 300

Query: 301 IALGSEMSGGIRNVRIENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMT 360
           IALGSEMSGGIRNVRIENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMT
Sbjct: 301 IALGSEMSGGIRNVRIENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMT 360

Query: 361 GNYKSHPDEKFDPAAVPEITNINYRDVVAENVTYSARLEGISGDPFTDICISNVKIGLTA 420
           GNYKSHPD+KFDP AVPEITNINYRDVVAENVTYSARLEGISGDPFTDICISNVKIGLTA
Sbjct: 361 GNYKSHPDDKFDPTAVPEITNINYRDVVAENVTYSARLEGISGDPFTDICISNVKIGLTA 420

Query: 421 KPKKLQWNCTDVEGFSSDVVPPPCAPLAKAAKGGGCYFPEDQLPIEKVQLKTCFVKNAA 480
           KPKKLQWNCTDVEGFSSDV+PPPCAPLAKAAK GGCYFPED+LPIEKVQLKTCFV+N A
Sbjct: 421 KPKKLQWNCTDVEGFSSDVMPPPCAPLAKAAKSGGCYFPEDELPIEKVQLKTCFVQNPA 479

BLAST of HG10008951 vs. NCBI nr
Match: XP_008437531.1 (PREDICTED: probable polygalacturonase [Cucumis melo] >KAA0042595.1 putative polygalacturonase [Cucumis melo var. makuwa] >TYK05998.1 putative polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 918.7 bits (2373), Expect = 2.1e-263
Identity = 442/477 (92.66%), Postives = 460/477 (96.44%), Query Frame = 0

Query: 1   MDHSPKPTKTHVTAARISAVIVLALVSLATVEGKKPCPTTNYLEVPAISCRKHTALLTDF 60
           MD   KPTKTH  AARISAVI+LALVSLATVEGK+ CP TNYL+VPAI+CR+HTA+LTDF
Sbjct: 1   MDLPQKPTKTHDAAARISAVILLALVSLATVEGKRQCP-TNYLQVPAINCRQHTAVLTDF 60

Query: 61  GGVGDGVTSNTHAFRQAIEHLSPLAADGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAKI 120
           GGVGDGVTSNT AFR+AIEHLSPLAA+GGAQLIVPPGKWLTGSFNLTSHFTLFVHKDA I
Sbjct: 61  GGVGDGVTSNTQAFRRAIEHLSPLAANGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDATI 120

Query: 121 LASQDESEWPLVAVLPSYGRGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGSYWWDK 180
           LASQDESEWP VA+LPSYG GRDAPGGRYSSLI+GTNLTDVVITGNNGTIDGQGSYWWDK
Sbjct: 121 LASQDESEWPQVAILPSYGVGRDAPGGRYSSLIYGTNLTDVVITGNNGTIDGQGSYWWDK 180

Query: 181 FHKNELNVTRPYLIEIIYSDQIQISDLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPN 240
           FHK ELN+TRPY+IEI+YSDQIQIS+LTLVNSPSWFVHPIYS+NVIIQGLTILAPIDSPN
Sbjct: 181 FHKGELNLTRPYMIEILYSDQIQISNLTLVNSPSWFVHPIYSKNVIIQGLTILAPIDSPN 240

Query: 241 TDGIDPDSCSNTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIRRLTCISPDSAG 300
           TDGIDPDSCSN RIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIRRLTCISPDSAG
Sbjct: 241 TDGIDPDSCSNIRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIRRLTCISPDSAG 300

Query: 301 IALGSEMSGGIRNVRIENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMT 360
           IALGSEMSGGIRNVRIENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMT
Sbjct: 301 IALGSEMSGGIRNVRIENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMT 360

Query: 361 GNYKSHPDEKFDPAAVPEITNINYRDVVAENVTYSARLEGISGDPFTDICISNVKIGLTA 420
           GNYKSHPDEKFDPAA+PEITNINYRDVVAENVTYSARLEGISGDPFT+ICISNVKIGLTA
Sbjct: 361 GNYKSHPDEKFDPAALPEITNINYRDVVAENVTYSARLEGISGDPFTNICISNVKIGLTA 420

Query: 421 KPKKLQWNCTDVEGFSSDVVPPPCAPLAKAAKGGGCYFPEDQLPIEKVQLKTCFVKN 478
           KPKKLQWNCTDVEG SSDVVPPPCAPLAKAAK GGC FP DQLPIEKVQLKTCFV+N
Sbjct: 421 KPKKLQWNCTDVEGISSDVVPPPCAPLAKAAKSGGCDFPADQLPIEKVQLKTCFVQN 476

BLAST of HG10008951 vs. NCBI nr
Match: XP_011654644.1 (probable polygalacturonase [Cucumis sativus] >KGN49922.1 hypothetical protein Csa_000303 [Cucumis sativus])

HSP 1 Score: 905.6 bits (2339), Expect = 1.8e-259
Identity = 436/473 (92.18%), Postives = 455/473 (96.19%), Query Frame = 0

Query: 7   PTKTHVTAARISAVIVLALVSLATVEGKKPCPTTNYLEVPAISCRKHTALLTDFGGVGDG 66
           PTKTH  AARISAVI+LAL+SLATVEGK+ CP TNYL+VPAISCRKHTAL+TDFGGVGDG
Sbjct: 7   PTKTHDAAARISAVILLALLSLATVEGKRSCP-TNYLQVPAISCRKHTALITDFGGVGDG 66

Query: 67  VTSNTHAFRQAIEHLSPLAADGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAKILASQ-- 126
           VTSNT AFR+AIEHLS LAADGGAQL VPPGKWLTGSFNLTSHFTLFVHKDAKIL SQ  
Sbjct: 67  VTSNTQAFRRAIEHLSSLAADGGAQLTVPPGKWLTGSFNLTSHFTLFVHKDAKILGSQVE 126

Query: 127 DESEWPLVAVLPSYGRGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGSYWWDKFHKN 186
           +ESEWP VA+LPSYG GRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQG+YWWDKFHK 
Sbjct: 127 NESEWPQVALLPSYGVGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGAYWWDKFHKG 186

Query: 187 ELNVTRPYLIEIIYSDQIQISDLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPNTDGI 246
           ELN+TRPYLIEI+YSDQIQISDLTLVNSPSWFVHPIYS+NVIIQGLTILAPIDSPNTDGI
Sbjct: 187 ELNLTRPYLIEIMYSDQIQISDLTLVNSPSWFVHPIYSKNVIIQGLTILAPIDSPNTDGI 246

Query: 247 DPDSCSNTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIRRLTCISPDSAGIALG 306
           DPDSCSNTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVI+RLTCISPDSAG+ALG
Sbjct: 247 DPDSCSNTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIKRLTCISPDSAGVALG 306

Query: 307 SEMSGGIRNVRIENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMTGNYK 366
           SEMSGGIRNVRIEN TGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMTGNYK
Sbjct: 307 SEMSGGIRNVRIENFTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMTGNYK 366

Query: 367 SHPDEKFDPAAVPEITNINYRDVVAENVTYSARLEGISGDPFTDICISNVKIGLTAKPKK 426
           SHPD+KFDPAA+PEITNINYRDVVAENVTYSARLEGISGDPFT+ICISNVKIGLTA PKK
Sbjct: 367 SHPDDKFDPAALPEITNINYRDVVAENVTYSARLEGISGDPFTNICISNVKIGLTATPKK 426

Query: 427 LQWNCTDVEGFSSDVVPPPCAPLAKAAKGGGCYFPEDQLPIEKVQLKTCFVKN 478
           LQWNCTDVEGFSSDVVPPPCAPLAKA K GGC FPEDQLPIE+VQLKTCFV+N
Sbjct: 427 LQWNCTDVEGFSSDVVPPPCAPLAKAGKSGGCDFPEDQLPIERVQLKTCFVQN 478

BLAST of HG10008951 vs. NCBI nr
Match: XP_023532553.1 (probable polygalacturonase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 886.3 bits (2289), Expect = 1.2e-253
Identity = 424/480 (88.33%), Postives = 451/480 (93.96%), Query Frame = 0

Query: 1   MDHSPKPTKTHVTAARISAVIVLALVSLATVEGKKPCPTTNYLEVPAISCRKHTALLTDF 60
           MD   KPTKT +TAAR+SAVI+LA VSL +V+GKKPCP ++YLE PAISCRKHTA+LTDF
Sbjct: 1   MDLPHKPTKTQITAARVSAVILLAFVSLGSVDGKKPCPFSSYLEFPAISCRKHTAVLTDF 60

Query: 61  GGVGDGVTSNTHAFRQAIEHLSPLAADGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAKI 120
           GGVGDGVTSNT+AFR+A+EHLS LA DGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDA+I
Sbjct: 61  GGVGDGVTSNTNAFREAMEHLSTLAVDGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAQI 120

Query: 121 LASQDESEWPLVAVLPSYGRGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGSYWWDK 180
           LASQDESEWPLV VLPSYGRGRDAPGGR+SSLIFGTNLTDVVITGNNGTIDGQGSYWWDK
Sbjct: 121 LASQDESEWPLVEVLPSYGRGRDAPGGRFSSLIFGTNLTDVVITGNNGTIDGQGSYWWDK 180

Query: 181 FHKNELNVTRPYLIEIIYSDQIQISDLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPN 240
           FH+NELNVTRPY+IEI+YSDQIQIS+LTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPN
Sbjct: 181 FHRNELNVTRPYMIEIMYSDQIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPN 240

Query: 241 TDGIDPDSCSNTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIRRLTCISPDSAG 300
           TDGIDPDSC NTRIEDCFIVSGDDCIAVKSGWDQYGI+FGMPTEDL+IRRLTCISPDSAG
Sbjct: 241 TDGIDPDSCINTRIEDCFIVSGDDCIAVKSGWDQYGIQFGMPTEDLLIRRLTCISPDSAG 300

Query: 301 IALGSEMSGGIRNVRIENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMT 360
           IALGSEMSGGIRNVRIENVT INTQSAVRIKTA GRGG+VKDIFVR M+LSTMKYVFWMT
Sbjct: 301 IALGSEMSGGIRNVRIENVTAINTQSAVRIKTAIGRGGYVKDIFVRQMFLSTMKYVFWMT 360

Query: 361 GNYKSHPDEKFDPAAVPEITNINYRDVVAENVTYSARLEGISGDPFTDICISNVKIGLTA 420
           GNYKSHPDEKFDP A+PEI NINYRDVVAENVTYSARL+GISGDPFTDICISNVKIGLTA
Sbjct: 361 GNYKSHPDEKFDPKALPEIKNINYRDVVAENVTYSARLDGISGDPFTDICISNVKIGLTA 420

Query: 421 KPKKLQWNCTDVEGFSSDVVPPPCAPLAK-AAKGGGCYFPEDQLPIEKVQLKTCFVKNAA 480
           KPKKLQWNCT+VEG+SSDVVPPPCAPLAK      GC FPED+LPIE V+L TC  KN A
Sbjct: 421 KPKKLQWNCTNVEGYSSDVVPPPCAPLAKEVVSSDGCPFPEDRLPIEDVKLMTCVTKNHA 480

BLAST of HG10008951 vs. NCBI nr
Match: XP_022996001.1 (probable polygalacturonase [Cucurbita maxima])

HSP 1 Score: 880.9 bits (2275), Expect = 4.8e-252
Identity = 424/480 (88.33%), Postives = 448/480 (93.33%), Query Frame = 0

Query: 1   MDHSPKPTKTHVTAARISAVIVLALVSLATVEGKKPCPTTNYLEVPAISCRKHTALLTDF 60
           MD   KPTKT +TA RISAVI+L LVSLA+V+GKKPCP ++YLE  AISCRKHTA+LTDF
Sbjct: 1   MDLPHKPTKTQITAVRISAVILLVLVSLASVDGKKPCPFSSYLEFSAISCRKHTAVLTDF 60

Query: 61  GGVGDGVTSNTHAFRQAIEHLSPLAADGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAKI 120
           GGVGDGVTSNT+AFR+AIEHLS LA DGG+QLIVPPGKWLTGSFNLTSHFTLFVHKDA+I
Sbjct: 61  GGVGDGVTSNTNAFREAIEHLSTLAIDGGSQLIVPPGKWLTGSFNLTSHFTLFVHKDAQI 120

Query: 121 LASQDESEWPLVAVLPSYGRGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGSYWWDK 180
           LASQDESEWPLV VLPSYGRGRDAPGGR+SSLIFGTNLTDVVITGNNGTIDGQGSYWWDK
Sbjct: 121 LASQDESEWPLVEVLPSYGRGRDAPGGRFSSLIFGTNLTDVVITGNNGTIDGQGSYWWDK 180

Query: 181 FHKNELNVTRPYLIEIIYSDQIQISDLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPN 240
           FHKNE NVTRPY+IEI+YSDQIQIS+LTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPN
Sbjct: 181 FHKNEFNVTRPYMIEIMYSDQIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPN 240

Query: 241 TDGIDPDSCSNTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIRRLTCISPDSAG 300
           TDGIDPDSC NTRIEDCFIVSGDDCIAVKSGWDQYGI+FGMPTEDL+IRRLTCISPDSAG
Sbjct: 241 TDGIDPDSCINTRIEDCFIVSGDDCIAVKSGWDQYGIQFGMPTEDLLIRRLTCISPDSAG 300

Query: 301 IALGSEMSGGIRNVRIENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMT 360
           IALGSEMSGGIRNVRIENVT INTQSAVRIKTA GRGG+VKDIFVR M+LSTMKYVFWMT
Sbjct: 301 IALGSEMSGGIRNVRIENVTAINTQSAVRIKTAIGRGGYVKDIFVRQMFLSTMKYVFWMT 360

Query: 361 GNYKSHPDEKFDPAAVPEITNINYRDVVAENVTYSARLEGISGDPFTDICISNVKIGLTA 420
           GNYKSHPDEKFDP A+PEI NINYRDVVAENVTYSARL+GISGDPFTDICISNVKIGLTA
Sbjct: 361 GNYKSHPDEKFDPKALPEIKNINYRDVVAENVTYSARLDGISGDPFTDICISNVKIGLTA 420

Query: 421 KPKKLQWNCTDVEGFSSDVVPPPCAPLAK-AAKGGGCYFPEDQLPIEKVQLKTCFVKNAA 480
           KPKKLQWNCT+VEG+SSDVVP PCAPLAK      GC FPEDQLPIE V+L TC  KN A
Sbjct: 421 KPKKLQWNCTNVEGYSSDVVPLPCAPLAKEVVSSDGCPFPEDQLPIEDVKLMTCITKNHA 480

BLAST of HG10008951 vs. ExPASy Swiss-Prot
Match: A7PZL3 (Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 SV=1)

HSP 1 Score: 634.0 bits (1634), Expect = 1.4e-180
Identity = 301/456 (66.01%), Postives = 360/456 (78.95%), Query Frame = 0

Query: 20  VIVLALVSLATVEGKKPCPTTNYLEVPAISCRKHTALLTDFGGVGDGVTSNTHAFRQAIE 79
           V V+ L+S++  E +K       LE  AISCR H+A L DFGGVGDG T NT AF+ A+ 
Sbjct: 29  VAVVLLLSVSRGECRKG-RILEALEYSAISCRAHSASLVDFGGVGDGQTLNTKAFQDAVS 88

Query: 80  HLSPLAADGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAKILASQDESEWPLVAVLPSYG 139
            LS   ++GGAQL VP GKWLTGSF+LTSHFTLF+H+DA +LASQD S+WP++  LPSYG
Sbjct: 89  ELSKYGSEGGAQLYVPAGKWLTGSFSLTSHFTLFLHRDAVLLASQDISQWPVIKPLPSYG 148

Query: 140 RGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGSYWWDKFHKNELNVTRPYLIEIIYS 199
           RGRDA  GRY+SLIFGTNLTDV+ITG+NGTIDGQG  WW +FH  +L  TRPYLIE++YS
Sbjct: 149 RGRDAAAGRYTSLIFGTNLTDVIITGDNGTIDGQGGLWWQRFHGGKLKYTRPYLIELMYS 208

Query: 200 DQIQISDLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPNTDGIDPDSCSNTRIEDCFI 259
             IQIS+LTL+NSPSW VHP+YSRN++IQG+TILAP+ SPNTDGI+PDSC+NTRIEDC+I
Sbjct: 209 ADIQISNLTLLNSPSWNVHPVYSRNILIQGITILAPVRSPNTDGINPDSCTNTRIEDCYI 268

Query: 260 VSGDDCIAVKSGWDQYGIKFGMPTEDLVIRRLTCISPDSAGIALGSEMSGGIRNVRIENV 319
           VSGDDC+AVKSGWD+YGI +GMPT+ LVIRRLTCISP SA IALGSEMSGGI++VR E++
Sbjct: 269 VSGDDCVAVKSGWDEYGIAYGMPTKQLVIRRLTCISPYSAVIALGSEMSGGIQDVRAEDI 328

Query: 320 TGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMTGNYKSHPDEKFDPAAVPEI 379
             IN++S +RIKT  GRGG+VKDI+VRGM + TMK+ FWMTGNY SH D  +DP A P I
Sbjct: 329 VAINSESGIRIKTGIGRGGYVKDIYVRGMTMKTMKWAFWMTGNYGSHADNHYDPKAFPVI 388

Query: 380 TNINYRDVVAENVTYSARLEGISGDPFTDICISNVKIGLTAKPKKLQWNCTDVEGFSSDV 439
             INYRD+VAENV+ +ARLEGI  DPFT ICISNV I L AK KK+ W CTDVEG SS V
Sbjct: 389 QGINYRDMVAENVSMAARLEGIPSDPFTGICISNVTIHLAAKAKKVPWTCTDVEGISSGV 448

Query: 440 VPPPCAPLAKAA--KGGGCYFPEDQLPIEKVQLKTC 474
            P PC+ L      K   C FP + LPI+ V+L+ C
Sbjct: 449 TPTPCSTLPDQGPEKTSLCNFPAESLPIDTVELQKC 483

BLAST of HG10008951 vs. ExPASy Swiss-Prot
Match: Q9LW07 (Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g15720 PE=3 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 4.2e-33
Identity = 120/403 (29.78%), Postives = 178/403 (44.17%), Query Frame = 0

Query: 57  LTDFGGVGDGVTSNTHAFRQAIEHLSPLAADGGAQLIVPPGKW-------LTGSFNLTSH 116
           +T FG VGDGVT ++ AF +A E +     DG  Q +VP G           GS   T  
Sbjct: 26  VTQFGAVGDGVTDDSQAFLKAWEAVCSGTGDG--QFVVPAGMTFMLQPLKFQGSCKSTPV 85

Query: 117 FTLFVHKDAKILASQDESEWPLVAVLPSYGRGRDAPGGRYSSLIFGTNLTDVVITGNNGT 176
           F   + K                 V PS G  +    G     I  T++  +VI G +G 
Sbjct: 86  FVQMLGK----------------LVAPSKGNWK----GDKDQWILFTDIEGLVIEG-DGE 145

Query: 177 IDGQGSYWWDKFHKNELNVTRPYLIEIIYSDQIQISDLTLVNSPSWFVHPIYSRNVIIQG 236
           I+GQGS WW+  HK     +RP  ++    + +++S LT ++SP   +H      V I  
Sbjct: 146 INGQGSSWWE--HKG----SRPTALKFRSCNNLRLSGLTHLDSPMAHIHISECNYVTISS 205

Query: 237 LTILAPIDSPNTDGIDPDSCSNTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIR 296
           L I AP  SPNTDGID  + SN  I+DC I +GDDCIA+ SG           T ++ I 
Sbjct: 206 LRINAPESSPNTDGIDVGASSNVVIQDCIIATGDDCIAINSG-----------TSNIHIS 265

Query: 297 RLTCISPDSAGIALGSEMSGG----IRNVRIENVTGINTQSAVRIKTARGRGGFVKDIFV 356
            + C      GI++GS    G    + NV ++N     T +  RIKT +G  G+ + I  
Sbjct: 266 GIDC--GPGHGISIGSLGKDGETATVENVCVQNCNFRGTMNGARIKTWQGGSGYARMITF 325

Query: 357 RGMYLSTMKY-----VFWMTGNYKSHPDEKFDPAAVPEITNINYRDVVAENVTYSARLEG 416
            G+ L  ++       F+  G+  +  D K     V ++   N+  +      Y      
Sbjct: 326 NGITLDNVENPIIIDQFYNGGDSDNAKDRKSSAVEVSKVVFSNF--IGTSKSEYGVDFRC 384

Query: 417 ISGDPFTDICISNVKI--GLTAKPKKLQWNCTDVEGFSSDVVP 442
               P T+I + ++KI    +   +  Q  C +V G S+  VP
Sbjct: 386 SERVPCTEIFLRDMKIETASSGSGQVAQGQCLNVRGASTIAVP 384

BLAST of HG10008951 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 1.1e-28
Identity = 117/407 (28.75%), Postives = 173/407 (42.51%), Query Frame = 0

Query: 60  FGGVGDGVTSNTHAFRQAIEHLSPLAADGGAQLIVPPGKWLTGS---FNLTSHFTLFVHK 119
           +G VGDG   +T AF       +       A L+VP  K    +   F       L    
Sbjct: 64  YGAVGDGKHDSTEAFATT---WNAACKKASAVLLVPANKKFFVNNLVFRGPCQPHLSFKV 123

Query: 120 DAKILASQDESEWPLVAVLPSYGRGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGSY 179
           D  I+A  D + W    +   + +                 LTD  + G  G IDGQG  
Sbjct: 124 DGTIVAQPDPARWKNSKIWLQFAQ-----------------LTDFNLMG-TGVIDGQGQQ 183

Query: 180 WWDKFHK--------NELNVTRPYLIEIIYSDQIQISDLTLVNSPSWFVHPIYSRNVIIQ 239
           WW    K        N+ N  RP  I+I YS  + + +LTL+NSP + +       V IQ
Sbjct: 184 WWAGQCKVVNGRTVCNDRN--RPTAIKIDYSKSVTVKELTLMNSPEFHLVFGECEGVKIQ 243

Query: 240 GLTILAPIDSPNTDGIDPDSCSNTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVI 299
           GL I AP DSPNTDGID  +     IE C I +GDDCIA+ +G           + ++ I
Sbjct: 244 GLKIKAPRDSPNTDGIDIFASKRFHIEKCVIGTGDDCIAIGTG-----------SSNITI 303

Query: 300 RRLTCISPDSAGIALGS----EMSGGIRNVRIENVTGINTQSAVRIKTARGRGGFVKDIF 359
           + L C      GI++GS         + +V +     I+TQ+ +RIKT +G  G    I 
Sbjct: 304 KDLIC--GPGHGISIGSLGRDNSRAEVSHVHVNRAKFIDTQNGLRIKTWQGGSGLASYIT 363

Query: 360 VRGMYLSTMKYVFWMTGNYKSHPDEKFDPAAVPEITNINYRDVVAENVTYSA-RLEGISG 419
              + +   +    +   Y +      +  +  +I  + Y+++   + T +A +L     
Sbjct: 364 YENVEMINSENPILINQFYCTSASACQNQRSAVQIQGVTYKNIHGTSATAAAIQLMCSDS 423

Query: 420 DPFTDICISNVKIGLTA-KPKKLQWNCTD--VEGFSSDVVPPPCAPL 448
            P T I +SNV + LT+ KP     +C D    GF S  + P C  L
Sbjct: 424 VPCTGIQLSNVSLKLTSGKPA----SCVDKNARGFYSGRLIPTCKNL 430

BLAST of HG10008951 vs. ExPASy Swiss-Prot
Match: Q9FY19 (Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 9.2e-28
Identity = 116/411 (28.22%), Postives = 182/411 (44.28%), Query Frame = 0

Query: 60  FGGVGDGVTSNTHAFRQ----AIEHLSPLAADGGAQLIVPPGKWLTGSFNLT------SH 119
           +G VGDG   +T AF +    A   LS       A  +VP  K    + NL        H
Sbjct: 65  YGAVGDGKHDSTDAFEKTWNAACNKLS-------AVFLVPANKKFVVN-NLVFYGPCQPH 124

Query: 120 FTLFVHKDAKILASQDESEWPLVAVLPSYGRGRDAPGGRYSSLIFGTNLTDVVITGNNGT 179
           F+  V  D  I A  D ++W    +   + R                 LTD  + G  G 
Sbjct: 125 FSFKV--DGTIAAYPDPAKWKNSKIWMHFAR-----------------LTDFNLMG-TGV 184

Query: 180 IDGQGSYWWDKFHKNELNVT------RPYLIEIIYSDQIQISDLTLVNSPSWFVHPIYSR 239
           IDGQG+ WW    K     T      RP  I+I +S  + + +LTL NSP +  H ++  
Sbjct: 185 IDGQGNRWWSDQCKTINGRTVCNDKGRPTAIKIDFSKSVTVKELTLTNSPEF--HLVFGE 244

Query: 240 --NVIIQGLTILAPIDSPNTDGIDPDSCSNTRIEDCFIVSGDDCIAVKSGWDQYGIKFGM 299
              V IQG+ I AP DSPNTDGID  +     IE C I +GDDC+AV +G          
Sbjct: 245 CDGVKIQGIKIKAPRDSPNTDGIDIFASKRFEIEKCTIGTGDDCVAVGTG---------- 304

Query: 300 PTEDLVIRRLTCISPDSAGIALGSEMSGGIRN----VRIENVTGINTQSAVRIKTARGRG 359
            + ++ I+ LTC      G+++GS   G  R+    V ++    I+TQ+ +RIKT +G  
Sbjct: 305 -SSNITIKDLTC--GPGHGMSIGSLGKGNSRSEVSFVHLDGAKFIDTQNGLRIKTWQGGS 364

Query: 360 GFVKDIFVRGMYLSTMKYVFWMTGNYKSHPDEKFDPAAVPEITNINYRDVVAENVTYSA- 419
           G    I    + +   +    +   Y +      +  +  +I ++ ++++   + T +A 
Sbjct: 365 GLASHITYENVEMINAENPILINQFYCTSAAACKNQRSAVKIQDVTFKNIHGTSATTAAI 424

Query: 420 RLEGISGDPFTDICISNVKIGLTAKPKKLQWNCTDVEGFSSDVVPPPCAPL 448
           +L      P ++I +SNV + LT+       N  +  G+ ++ + P C  L
Sbjct: 425 QLMCSDSVPCSNIKLSNVFLKLTSGKVATCVN-KNANGYYTNPLNPSCKSL 431

BLAST of HG10008951 vs. ExPASy Swiss-Prot
Match: P43212 (Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 9.5e-25
Identity = 111/409 (27.14%), Postives = 175/409 (42.79%), Query Frame = 0

Query: 60  FGGVGDGVTSNTHAFRQAIEHLSPLAADGGAQLIVPPGKWLTGS---FN--LTSHFTLFV 119
           +G VGDG    T AF  A +          A L+VP  K    +   FN     HFT  V
Sbjct: 64  YGAVGDGKHDCTEAFSTAWQ---AACKKPSAMLLVPGNKKFVVNNLFFNGPCQPHFTFKV 123

Query: 120 HKDAKILASQDESEWPLVAVLPSYGRGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQG 179
             D  I A Q+ + W    +   + +                 LT   + G  G IDGQG
Sbjct: 124 --DGIIAAYQNPASWKNNRIWLQFAK-----------------LTGFTLMG-KGVIDGQG 183

Query: 180 SYWWDKFHK----NEL--NVTRPYLIEIIYSDQIQISDLTLVNSPSWFVHPIYSRNVIIQ 239
             WW    K     E+  +  RP  I+  +S  + I  L L+NSP + +       V I 
Sbjct: 184 KQWWAGQCKWVNGREICNDRDRPTAIKFDFSTGLIIQGLKLMNSPEFHLVFGNCEGVKII 243

Query: 240 GLTILAPIDSPNTDGIDPDSCSNTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVI 299
           G++I AP DSPNTDGID  +  N  ++   I +GDDC+A+ +G           + ++VI
Sbjct: 244 GISITAPRDSPNTDGIDIFASKNFHLQKNTIGTGDDCVAIGTG-----------SSNIVI 303

Query: 300 RRLTCISPDSAGIALGS----EMSGGIRNVRIENVTGINTQSAVRIKTARGRGGFVKDIF 359
             L C      GI++GS         +  V +     I+TQ+ +RIKT +G  G    I 
Sbjct: 304 EDLIC--GPGHGISIGSLGRENSRAEVSYVHVNGAKFIDTQNGLRIKTWQGGSGMASHII 363

Query: 360 VRGMYLSTMKYVFWMTGNYKSHPDEKFDPAAVPEITNINYRDVVAENVTYSA-RLEGISG 419
              + +   +    +   Y +      +  +  +I ++ Y+++   + T +A +L+    
Sbjct: 364 YENVEMINSENPILINQFYCTSASACQNQRSAVQIQDVTYKNIRGTSATAAAIQLKCSDS 423

Query: 420 DPFTDICISNVKIGLTAKPKKLQWNCTDVEGFSSDVVPPPCAPLAKAAK 453
            P  DI +S++ + LT+       N  +  G+ S  V P C  L+ +AK
Sbjct: 424 MPCKDIKLSDISLKLTSGKIASCLN-DNANGYFSGHVIPACKNLSPSAK 435

BLAST of HG10008951 vs. ExPASy TrEMBL
Match: A0A5D3C461 (Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G00950 PE=3 SV=1)

HSP 1 Score: 918.7 bits (2373), Expect = 1.0e-263
Identity = 442/477 (92.66%), Postives = 460/477 (96.44%), Query Frame = 0

Query: 1   MDHSPKPTKTHVTAARISAVIVLALVSLATVEGKKPCPTTNYLEVPAISCRKHTALLTDF 60
           MD   KPTKTH  AARISAVI+LALVSLATVEGK+ CP TNYL+VPAI+CR+HTA+LTDF
Sbjct: 1   MDLPQKPTKTHDAAARISAVILLALVSLATVEGKRQCP-TNYLQVPAINCRQHTAVLTDF 60

Query: 61  GGVGDGVTSNTHAFRQAIEHLSPLAADGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAKI 120
           GGVGDGVTSNT AFR+AIEHLSPLAA+GGAQLIVPPGKWLTGSFNLTSHFTLFVHKDA I
Sbjct: 61  GGVGDGVTSNTQAFRRAIEHLSPLAANGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDATI 120

Query: 121 LASQDESEWPLVAVLPSYGRGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGSYWWDK 180
           LASQDESEWP VA+LPSYG GRDAPGGRYSSLI+GTNLTDVVITGNNGTIDGQGSYWWDK
Sbjct: 121 LASQDESEWPQVAILPSYGVGRDAPGGRYSSLIYGTNLTDVVITGNNGTIDGQGSYWWDK 180

Query: 181 FHKNELNVTRPYLIEIIYSDQIQISDLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPN 240
           FHK ELN+TRPY+IEI+YSDQIQIS+LTLVNSPSWFVHPIYS+NVIIQGLTILAPIDSPN
Sbjct: 181 FHKGELNLTRPYMIEILYSDQIQISNLTLVNSPSWFVHPIYSKNVIIQGLTILAPIDSPN 240

Query: 241 TDGIDPDSCSNTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIRRLTCISPDSAG 300
           TDGIDPDSCSN RIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIRRLTCISPDSAG
Sbjct: 241 TDGIDPDSCSNIRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIRRLTCISPDSAG 300

Query: 301 IALGSEMSGGIRNVRIENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMT 360
           IALGSEMSGGIRNVRIENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMT
Sbjct: 301 IALGSEMSGGIRNVRIENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMT 360

Query: 361 GNYKSHPDEKFDPAAVPEITNINYRDVVAENVTYSARLEGISGDPFTDICISNVKIGLTA 420
           GNYKSHPDEKFDPAA+PEITNINYRDVVAENVTYSARLEGISGDPFT+ICISNVKIGLTA
Sbjct: 361 GNYKSHPDEKFDPAALPEITNINYRDVVAENVTYSARLEGISGDPFTNICISNVKIGLTA 420

Query: 421 KPKKLQWNCTDVEGFSSDVVPPPCAPLAKAAKGGGCYFPEDQLPIEKVQLKTCFVKN 478
           KPKKLQWNCTDVEG SSDVVPPPCAPLAKAAK GGC FP DQLPIEKVQLKTCFV+N
Sbjct: 421 KPKKLQWNCTDVEGISSDVVPPPCAPLAKAAKSGGCDFPADQLPIEKVQLKTCFVQN 476

BLAST of HG10008951 vs. ExPASy TrEMBL
Match: A0A1S3AUV5 (probable polygalacturonase OS=Cucumis melo OX=3656 GN=LOC103482916 PE=3 SV=1)

HSP 1 Score: 918.7 bits (2373), Expect = 1.0e-263
Identity = 442/477 (92.66%), Postives = 460/477 (96.44%), Query Frame = 0

Query: 1   MDHSPKPTKTHVTAARISAVIVLALVSLATVEGKKPCPTTNYLEVPAISCRKHTALLTDF 60
           MD   KPTKTH  AARISAVI+LALVSLATVEGK+ CP TNYL+VPAI+CR+HTA+LTDF
Sbjct: 1   MDLPQKPTKTHDAAARISAVILLALVSLATVEGKRQCP-TNYLQVPAINCRQHTAVLTDF 60

Query: 61  GGVGDGVTSNTHAFRQAIEHLSPLAADGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAKI 120
           GGVGDGVTSNT AFR+AIEHLSPLAA+GGAQLIVPPGKWLTGSFNLTSHFTLFVHKDA I
Sbjct: 61  GGVGDGVTSNTQAFRRAIEHLSPLAANGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDATI 120

Query: 121 LASQDESEWPLVAVLPSYGRGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGSYWWDK 180
           LASQDESEWP VA+LPSYG GRDAPGGRYSSLI+GTNLTDVVITGNNGTIDGQGSYWWDK
Sbjct: 121 LASQDESEWPQVAILPSYGVGRDAPGGRYSSLIYGTNLTDVVITGNNGTIDGQGSYWWDK 180

Query: 181 FHKNELNVTRPYLIEIIYSDQIQISDLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPN 240
           FHK ELN+TRPY+IEI+YSDQIQIS+LTLVNSPSWFVHPIYS+NVIIQGLTILAPIDSPN
Sbjct: 181 FHKGELNLTRPYMIEILYSDQIQISNLTLVNSPSWFVHPIYSKNVIIQGLTILAPIDSPN 240

Query: 241 TDGIDPDSCSNTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIRRLTCISPDSAG 300
           TDGIDPDSCSN RIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIRRLTCISPDSAG
Sbjct: 241 TDGIDPDSCSNIRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIRRLTCISPDSAG 300

Query: 301 IALGSEMSGGIRNVRIENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMT 360
           IALGSEMSGGIRNVRIENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMT
Sbjct: 301 IALGSEMSGGIRNVRIENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMT 360

Query: 361 GNYKSHPDEKFDPAAVPEITNINYRDVVAENVTYSARLEGISGDPFTDICISNVKIGLTA 420
           GNYKSHPDEKFDPAA+PEITNINYRDVVAENVTYSARLEGISGDPFT+ICISNVKIGLTA
Sbjct: 361 GNYKSHPDEKFDPAALPEITNINYRDVVAENVTYSARLEGISGDPFTNICISNVKIGLTA 420

Query: 421 KPKKLQWNCTDVEGFSSDVVPPPCAPLAKAAKGGGCYFPEDQLPIEKVQLKTCFVKN 478
           KPKKLQWNCTDVEG SSDVVPPPCAPLAKAAK GGC FP DQLPIEKVQLKTCFV+N
Sbjct: 421 KPKKLQWNCTDVEGISSDVVPPPCAPLAKAAKSGGCDFPADQLPIEKVQLKTCFVQN 476

BLAST of HG10008951 vs. ExPASy TrEMBL
Match: A0A0A0KNF9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G140480 PE=3 SV=1)

HSP 1 Score: 905.6 bits (2339), Expect = 8.9e-260
Identity = 436/473 (92.18%), Postives = 455/473 (96.19%), Query Frame = 0

Query: 7   PTKTHVTAARISAVIVLALVSLATVEGKKPCPTTNYLEVPAISCRKHTALLTDFGGVGDG 66
           PTKTH  AARISAVI+LAL+SLATVEGK+ CP TNYL+VPAISCRKHTAL+TDFGGVGDG
Sbjct: 7   PTKTHDAAARISAVILLALLSLATVEGKRSCP-TNYLQVPAISCRKHTALITDFGGVGDG 66

Query: 67  VTSNTHAFRQAIEHLSPLAADGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAKILASQ-- 126
           VTSNT AFR+AIEHLS LAADGGAQL VPPGKWLTGSFNLTSHFTLFVHKDAKIL SQ  
Sbjct: 67  VTSNTQAFRRAIEHLSSLAADGGAQLTVPPGKWLTGSFNLTSHFTLFVHKDAKILGSQVE 126

Query: 127 DESEWPLVAVLPSYGRGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGSYWWDKFHKN 186
           +ESEWP VA+LPSYG GRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQG+YWWDKFHK 
Sbjct: 127 NESEWPQVALLPSYGVGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGAYWWDKFHKG 186

Query: 187 ELNVTRPYLIEIIYSDQIQISDLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPNTDGI 246
           ELN+TRPYLIEI+YSDQIQISDLTLVNSPSWFVHPIYS+NVIIQGLTILAPIDSPNTDGI
Sbjct: 187 ELNLTRPYLIEIMYSDQIQISDLTLVNSPSWFVHPIYSKNVIIQGLTILAPIDSPNTDGI 246

Query: 247 DPDSCSNTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIRRLTCISPDSAGIALG 306
           DPDSCSNTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVI+RLTCISPDSAG+ALG
Sbjct: 247 DPDSCSNTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIKRLTCISPDSAGVALG 306

Query: 307 SEMSGGIRNVRIENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMTGNYK 366
           SEMSGGIRNVRIEN TGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMTGNYK
Sbjct: 307 SEMSGGIRNVRIENFTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMTGNYK 366

Query: 367 SHPDEKFDPAAVPEITNINYRDVVAENVTYSARLEGISGDPFTDICISNVKIGLTAKPKK 426
           SHPD+KFDPAA+PEITNINYRDVVAENVTYSARLEGISGDPFT+ICISNVKIGLTA PKK
Sbjct: 367 SHPDDKFDPAALPEITNINYRDVVAENVTYSARLEGISGDPFTNICISNVKIGLTATPKK 426

Query: 427 LQWNCTDVEGFSSDVVPPPCAPLAKAAKGGGCYFPEDQLPIEKVQLKTCFVKN 478
           LQWNCTDVEGFSSDVVPPPCAPLAKA K GGC FPEDQLPIE+VQLKTCFV+N
Sbjct: 427 LQWNCTDVEGFSSDVVPPPCAPLAKAGKSGGCDFPEDQLPIERVQLKTCFVQN 478

BLAST of HG10008951 vs. ExPASy TrEMBL
Match: A0A6J1K3G8 (probable polygalacturonase OS=Cucurbita maxima OX=3661 GN=LOC111491343 PE=3 SV=1)

HSP 1 Score: 880.9 bits (2275), Expect = 2.3e-252
Identity = 424/480 (88.33%), Postives = 448/480 (93.33%), Query Frame = 0

Query: 1   MDHSPKPTKTHVTAARISAVIVLALVSLATVEGKKPCPTTNYLEVPAISCRKHTALLTDF 60
           MD   KPTKT +TA RISAVI+L LVSLA+V+GKKPCP ++YLE  AISCRKHTA+LTDF
Sbjct: 1   MDLPHKPTKTQITAVRISAVILLVLVSLASVDGKKPCPFSSYLEFSAISCRKHTAVLTDF 60

Query: 61  GGVGDGVTSNTHAFRQAIEHLSPLAADGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAKI 120
           GGVGDGVTSNT+AFR+AIEHLS LA DGG+QLIVPPGKWLTGSFNLTSHFTLFVHKDA+I
Sbjct: 61  GGVGDGVTSNTNAFREAIEHLSTLAIDGGSQLIVPPGKWLTGSFNLTSHFTLFVHKDAQI 120

Query: 121 LASQDESEWPLVAVLPSYGRGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGSYWWDK 180
           LASQDESEWPLV VLPSYGRGRDAPGGR+SSLIFGTNLTDVVITGNNGTIDGQGSYWWDK
Sbjct: 121 LASQDESEWPLVEVLPSYGRGRDAPGGRFSSLIFGTNLTDVVITGNNGTIDGQGSYWWDK 180

Query: 181 FHKNELNVTRPYLIEIIYSDQIQISDLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPN 240
           FHKNE NVTRPY+IEI+YSDQIQIS+LTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPN
Sbjct: 181 FHKNEFNVTRPYMIEIMYSDQIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPN 240

Query: 241 TDGIDPDSCSNTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIRRLTCISPDSAG 300
           TDGIDPDSC NTRIEDCFIVSGDDCIAVKSGWDQYGI+FGMPTEDL+IRRLTCISPDSAG
Sbjct: 241 TDGIDPDSCINTRIEDCFIVSGDDCIAVKSGWDQYGIQFGMPTEDLLIRRLTCISPDSAG 300

Query: 301 IALGSEMSGGIRNVRIENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMT 360
           IALGSEMSGGIRNVRIENVT INTQSAVRIKTA GRGG+VKDIFVR M+LSTMKYVFWMT
Sbjct: 301 IALGSEMSGGIRNVRIENVTAINTQSAVRIKTAIGRGGYVKDIFVRQMFLSTMKYVFWMT 360

Query: 361 GNYKSHPDEKFDPAAVPEITNINYRDVVAENVTYSARLEGISGDPFTDICISNVKIGLTA 420
           GNYKSHPDEKFDP A+PEI NINYRDVVAENVTYSARL+GISGDPFTDICISNVKIGLTA
Sbjct: 361 GNYKSHPDEKFDPKALPEIKNINYRDVVAENVTYSARLDGISGDPFTDICISNVKIGLTA 420

Query: 421 KPKKLQWNCTDVEGFSSDVVPPPCAPLAK-AAKGGGCYFPEDQLPIEKVQLKTCFVKNAA 480
           KPKKLQWNCT+VEG+SSDVVP PCAPLAK      GC FPEDQLPIE V+L TC  KN A
Sbjct: 421 KPKKLQWNCTNVEGYSSDVVPLPCAPLAKEVVSSDGCPFPEDQLPIEDVKLMTCITKNHA 480

BLAST of HG10008951 vs. ExPASy TrEMBL
Match: A0A6J1H0R5 (probable polygalacturonase OS=Cucurbita moschata OX=3662 GN=LOC111459096 PE=3 SV=1)

HSP 1 Score: 871.7 bits (2251), Expect = 1.4e-249
Identity = 417/480 (86.88%), Postives = 447/480 (93.12%), Query Frame = 0

Query: 1   MDHSPKPTKTHVTAARISAVIVLALVSLATVEGKKPCPTTNYLEVPAISCRKHTALLTDF 60
           MD   KPTKT +TAARIS VI+LALVSL +V+GKK CP ++YLE PAISCRKHTA+LTDF
Sbjct: 1   MDLPHKPTKTQITAARISVVILLALVSLGSVDGKKSCPFSSYLEFPAISCRKHTAVLTDF 60

Query: 61  GGVGDGVTSNTHAFRQAIEHLSPLAADGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAKI 120
           GG+GDGVTSNT+AFR+A+EHLS LA DGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDA+I
Sbjct: 61  GGIGDGVTSNTNAFREAMEHLSTLAVDGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAQI 120

Query: 121 LASQDESEWPLVAVLPSYGRGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGSYWWDK 180
           LASQDESEWPLV VLPSYGRGRDAP GR+SSLIFGTNLTDVVITGNNGTIDGQGSYWWDK
Sbjct: 121 LASQDESEWPLVEVLPSYGRGRDAPDGRFSSLIFGTNLTDVVITGNNGTIDGQGSYWWDK 180

Query: 181 FHKNELNVTRPYLIEIIYSDQIQISDLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPN 240
           FHKNE NVTRPY+IEI+YS+QIQIS+LTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPN
Sbjct: 181 FHKNEFNVTRPYMIEIMYSNQIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPN 240

Query: 241 TDGIDPDSCSNTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIRRLTCISPDSAG 300
           TDGIDPDSC NTRIEDCFIVSGDDCIAVKSGWDQYGI+FGMPTEDL+IRRLTCISPDSAG
Sbjct: 241 TDGIDPDSCINTRIEDCFIVSGDDCIAVKSGWDQYGIQFGMPTEDLLIRRLTCISPDSAG 300

Query: 301 IALGSEMSGGIRNVRIENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMT 360
           IALGSEMSGGIRNVRIENVT INTQSAVRIKTA GRGG+VKDIFVR M+LSTMKYVFWMT
Sbjct: 301 IALGSEMSGGIRNVRIENVTAINTQSAVRIKTAIGRGGYVKDIFVRQMFLSTMKYVFWMT 360

Query: 361 GNYKSHPDEKFDPAAVPEITNINYRDVVAENVTYSARLEGISGDPFTDICISNVKIGLTA 420
           G+YKSHPDEKFDP A+PEI NINYRDVVAENVTYSARL+GISGDPFTDICISN+KIGLTA
Sbjct: 361 GDYKSHPDEKFDPKALPEIKNINYRDVVAENVTYSARLDGISGDPFTDICISNIKIGLTA 420

Query: 421 KPKKLQWNCTDVEGFSSDVVPPPCAPLAK-AAKGGGCYFPEDQLPIEKVQLKTCFVKNAA 480
           K KKLQWNCT+VEG+SSDVVPPPCAPLAK      GC FPED+LPIE ++L TC  KN A
Sbjct: 421 KQKKLQWNCTNVEGYSSDVVPPPCAPLAKEVVSSDGCPFPEDRLPIEDLKLMTCVTKNHA 480

BLAST of HG10008951 vs. TAIR 10
Match: AT3G48950.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 658.3 bits (1697), Expect = 4.8e-189
Identity = 312/461 (67.68%), Postives = 372/461 (80.69%), Query Frame = 0

Query: 20  VIVLALVSLATVEGKK-PCPTTNYLEVPAISCRKHTALLTDFGGVGDGVTSNTHAFRQAI 79
           ++ LA+     +E +      T+ +E  A++CRKH+A+LTDFG VGDG TSNT AFR AI
Sbjct: 9   LVFLAIFLFPAIESRSHRNSVTSKIEFSALNCRKHSAILTDFGAVGDGKTSNTKAFRNAI 68

Query: 80  EHLSPLAADGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAKILASQDESEWPLVAVLPSY 139
             LS +A DGGAQL+VPPGKWLTGSFNLTSHFTLF+ + A ILASQDESEWP++A LPSY
Sbjct: 69  SKLSQMATDGGAQLVVPPGKWLTGSFNLTSHFTLFIQRGATILASQDESEWPVIAPLPSY 128

Query: 140 GRGRDAPG-GRYSSLIFGTNLTDVVITGNNGTIDGQGSYWWDKFHKNELNVTRPYLIEII 199
           G+GRD  G GR++SLI GTNLTDVVITGNNGTI+GQG YWWDKF K +  +TRPYLIEI+
Sbjct: 129 GKGRDGTGTGRFNSLISGTNLTDVVITGNNGTINGQGQYWWDKFKKKQFKITRPYLIEIL 188

Query: 200 YSDQIQISDLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPNTDGIDPDSCSNTRIEDC 259
           +S  IQIS++TL++SPSW +HP+Y  +VI++ +T+LAP+  PNTDGI+PDSC+NT IEDC
Sbjct: 189 FSKNIQISNITLIDSPSWNIHPVYCNSVIVKSVTVLAPVTVPNTDGINPDSCTNTLIEDC 248

Query: 260 FIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIRRLTCISPDSAGIALGSEMSGGIRNVRIE 319
           +IVSGDDCIAVKSGWDQYGIKFGMPT+ L IRRLTCISP SAG+ALGSEMSGGI++VRIE
Sbjct: 249 YIVSGDDCIAVKSGWDQYGIKFGMPTQQLSIRRLTCISPKSAGVALGSEMSGGIKDVRIE 308

Query: 320 NVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMTGNYKSHPDEKFDPAAVP 379
           +VT  NT+SA+RIKTA GRG +VKDI+ R + + TMKYVFWM+GNY SHPDE FDP A+P
Sbjct: 309 DVTLTNTESAIRIKTAVGRGAYVKDIYARRITMKTMKYVFWMSGNYGSHPDEGFDPKALP 368

Query: 380 EITNINYRDVVAENVTYSARLEGISGDPFTDICISNVKIGLTAKPKKLQWNCTDVEGFSS 439
           EITNINYRD+ AENVT SA L+GI  DPFT ICISNV I L AK KK+QWNCTDV G +S
Sbjct: 369 EITNINYRDMTAENVTMSASLDGIDKDPFTGICISNVTIALAAKAKKMQWNCTDVAGVTS 428

Query: 440 DVVPPPCA--PLAKA-AKGGGCYFPEDQLPIEKVQLKTCFV 476
            V P PC+  P  KA AK   C FP D +PIE V LK CF+
Sbjct: 429 RVTPEPCSLLPEKKAQAKNVDCAFPSDLIPIESVVLKKCFL 469

BLAST of HG10008951 vs. TAIR 10
Match: AT3G61490.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 640.6 bits (1651), Expect = 1.0e-183
Identity = 299/463 (64.58%), Postives = 369/463 (79.70%), Query Frame = 0

Query: 20  VIVLALVSLATV-EGKKPCPTTNYLEVPAISCRKHTALLTDFGGVGDGVTSNTHAFRQAI 79
           V ++ L +L T+ +G++   +    E  AI CR H+A +T++GGVGDG T NT AF+ A+
Sbjct: 9   VTIITLSNLITISQGRRVSQSFETFEYTAIICRSHSASITEYGGVGDGKTLNTKAFQSAV 68

Query: 80  EHLSPLAADGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAKILASQDESEWPLVAVLPSY 139
           +HLS  +++GGAQL VP GKWLTGSFNLTSHFTLF+HKDA +LA+QD +E+P++  LPSY
Sbjct: 69  DHLSQYSSEGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAILLAAQDLNEYPILKALPSY 128

Query: 140 GRGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGSYWWDKFHKNELNVTRPYLIEIIY 199
           GRGRDA GGR++SLIFGTNL+DV+ITGNNGTIDGQGS+WW KFH  +L  TRPYLIE+++
Sbjct: 129 GRGRDAAGGRFASLIFGTNLSDVIITGNNGTIDGQGSFWWQKFHGGKLKYTRPYLIELMF 188

Query: 200 SDQIQISDLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPNTDGIDPDSCSNTRIEDCF 259
           SD IQIS+LT ++SPSW +HP+YS N+I++G+TI+AP+ SPNTDGI+PDSC+NTRIEDC+
Sbjct: 189 SDTIQISNLTFLDSPSWNIHPVYSSNIIVKGVTIIAPVKSPNTDGINPDSCTNTRIEDCY 248

Query: 260 IVSGDDCIAVKSGWDQYGIKFGMPTEDLVIRRLTCISPDSAGIALGSEMSGGIRNVRIEN 319
           I+SGDDCIAVKSGWD+YGI FGMPT+ LVIRRLTCISP SA IALGSEMSGGI +VR E+
Sbjct: 249 IISGDDCIAVKSGWDEYGISFGMPTKHLVIRRLTCISPYSAAIALGSEMSGGIEDVRAED 308

Query: 320 VTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMTGNYKSHPDEKFDPAAVPE 379
           +T   T+S VRIKTA GRG FVK+I+V+GM L TMK+VFWMTGNYK+H D  +DP A+PE
Sbjct: 309 ITAYQTESGVRIKTAVGRGAFVKNIYVKGMNLHTMKWVFWMTGNYKAHADSHYDPHALPE 368

Query: 380 ITNINYRDVVAENVTYSARLEGISGDPFTDICISNVKIGLTAKPKKLQWNCTDVEGFSSD 439
           IT INYRD+VAENV+ + RLEGISGDPFT ICISN  I + AK KK  W C+DVEG +S 
Sbjct: 369 ITGINYRDIVAENVSMAGRLEGISGDPFTGICISNATISMAAKHKKAIWMCSDVEGVTSG 428

Query: 440 VVPPPCAPL--------AKAAKGGGCYFPEDQLPIEKVQLKTC 474
           V P PC  L         K    GGC FP D L I+ V+LKTC
Sbjct: 429 VDPKPCDLLDGQESETTKKKMIDGGCDFPTDVLEIDNVELKTC 471

BLAST of HG10008951 vs. TAIR 10
Match: AT3G61490.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 640.6 bits (1651), Expect = 1.0e-183
Identity = 299/463 (64.58%), Postives = 369/463 (79.70%), Query Frame = 0

Query: 20  VIVLALVSLATV-EGKKPCPTTNYLEVPAISCRKHTALLTDFGGVGDGVTSNTHAFRQAI 79
           V ++ L +L T+ +G++   +    E  AI CR H+A +T++GGVGDG T NT AF+ A+
Sbjct: 9   VTIITLSNLITISQGRRVSQSFETFEYTAIICRSHSASITEYGGVGDGKTLNTKAFQSAV 68

Query: 80  EHLSPLAADGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAKILASQDESEWPLVAVLPSY 139
           +HLS  +++GGAQL VP GKWLTGSFNLTSHFTLF+HKDA +LA+QD +E+P++  LPSY
Sbjct: 69  DHLSQYSSEGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAILLAAQDLNEYPILKALPSY 128

Query: 140 GRGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGSYWWDKFHKNELNVTRPYLIEIIY 199
           GRGRDA GGR++SLIFGTNL+DV+ITGNNGTIDGQGS+WW KFH  +L  TRPYLIE+++
Sbjct: 129 GRGRDAAGGRFASLIFGTNLSDVIITGNNGTIDGQGSFWWQKFHGGKLKYTRPYLIELMF 188

Query: 200 SDQIQISDLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPNTDGIDPDSCSNTRIEDCF 259
           SD IQIS+LT ++SPSW +HP+YS N+I++G+TI+AP+ SPNTDGI+PDSC+NTRIEDC+
Sbjct: 189 SDTIQISNLTFLDSPSWNIHPVYSSNIIVKGVTIIAPVKSPNTDGINPDSCTNTRIEDCY 248

Query: 260 IVSGDDCIAVKSGWDQYGIKFGMPTEDLVIRRLTCISPDSAGIALGSEMSGGIRNVRIEN 319
           I+SGDDCIAVKSGWD+YGI FGMPT+ LVIRRLTCISP SA IALGSEMSGGI +VR E+
Sbjct: 249 IISGDDCIAVKSGWDEYGISFGMPTKHLVIRRLTCISPYSAAIALGSEMSGGIEDVRAED 308

Query: 320 VTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMTGNYKSHPDEKFDPAAVPE 379
           +T   T+S VRIKTA GRG FVK+I+V+GM L TMK+VFWMTGNYK+H D  +DP A+PE
Sbjct: 309 ITAYQTESGVRIKTAVGRGAFVKNIYVKGMNLHTMKWVFWMTGNYKAHADSHYDPHALPE 368

Query: 380 ITNINYRDVVAENVTYSARLEGISGDPFTDICISNVKIGLTAKPKKLQWNCTDVEGFSSD 439
           IT INYRD+VAENV+ + RLEGISGDPFT ICISN  I + AK KK  W C+DVEG +S 
Sbjct: 369 ITGINYRDIVAENVSMAGRLEGISGDPFTGICISNATISMAAKHKKAIWMCSDVEGVTSG 428

Query: 440 VVPPPCAPL--------AKAAKGGGCYFPEDQLPIEKVQLKTC 474
           V P PC  L         K    GGC FP D L I+ V+LKTC
Sbjct: 429 VDPKPCDLLDGQESETTKKKMIDGGCDFPTDVLEIDNVELKTC 471

BLAST of HG10008951 vs. TAIR 10
Match: AT3G61490.3 (Pectin lyase-like superfamily protein )

HSP 1 Score: 640.6 bits (1651), Expect = 1.0e-183
Identity = 299/463 (64.58%), Postives = 369/463 (79.70%), Query Frame = 0

Query: 20  VIVLALVSLATV-EGKKPCPTTNYLEVPAISCRKHTALLTDFGGVGDGVTSNTHAFRQAI 79
           V ++ L +L T+ +G++   +    E  AI CR H+A +T++GGVGDG T NT AF+ A+
Sbjct: 9   VTIITLSNLITISQGRRVSQSFETFEYTAIICRSHSASITEYGGVGDGKTLNTKAFQSAV 68

Query: 80  EHLSPLAADGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAKILASQDESEWPLVAVLPSY 139
           +HLS  +++GGAQL VP GKWLTGSFNLTSHFTLF+HKDA +LA+QD +E+P++  LPSY
Sbjct: 69  DHLSQYSSEGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAILLAAQDLNEYPILKALPSY 128

Query: 140 GRGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGSYWWDKFHKNELNVTRPYLIEIIY 199
           GRGRDA GGR++SLIFGTNL+DV+ITGNNGTIDGQGS+WW KFH  +L  TRPYLIE+++
Sbjct: 129 GRGRDAAGGRFASLIFGTNLSDVIITGNNGTIDGQGSFWWQKFHGGKLKYTRPYLIELMF 188

Query: 200 SDQIQISDLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPNTDGIDPDSCSNTRIEDCF 259
           SD IQIS+LT ++SPSW +HP+YS N+I++G+TI+AP+ SPNTDGI+PDSC+NTRIEDC+
Sbjct: 189 SDTIQISNLTFLDSPSWNIHPVYSSNIIVKGVTIIAPVKSPNTDGINPDSCTNTRIEDCY 248

Query: 260 IVSGDDCIAVKSGWDQYGIKFGMPTEDLVIRRLTCISPDSAGIALGSEMSGGIRNVRIEN 319
           I+SGDDCIAVKSGWD+YGI FGMPT+ LVIRRLTCISP SA IALGSEMSGGI +VR E+
Sbjct: 249 IISGDDCIAVKSGWDEYGISFGMPTKHLVIRRLTCISPYSAAIALGSEMSGGIEDVRAED 308

Query: 320 VTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMTGNYKSHPDEKFDPAAVPE 379
           +T   T+S VRIKTA GRG FVK+I+V+GM L TMK+VFWMTGNYK+H D  +DP A+PE
Sbjct: 309 ITAYQTESGVRIKTAVGRGAFVKNIYVKGMNLHTMKWVFWMTGNYKAHADSHYDPHALPE 368

Query: 380 ITNINYRDVVAENVTYSARLEGISGDPFTDICISNVKIGLTAKPKKLQWNCTDVEGFSSD 439
           IT INYRD+VAENV+ + RLEGISGDPFT ICISN  I + AK KK  W C+DVEG +S 
Sbjct: 369 ITGINYRDIVAENVSMAGRLEGISGDPFTGICISNATISMAAKHKKAIWMCSDVEGVTSG 428

Query: 440 VVPPPCAPL--------AKAAKGGGCYFPEDQLPIEKVQLKTC 474
           V P PC  L         K    GGC FP D L I+ V+LKTC
Sbjct: 429 VDPKPCDLLDGQESETTKKKMIDGGCDFPTDVLEIDNVELKTC 471

BLAST of HG10008951 vs. TAIR 10
Match: AT2G23900.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 637.5 bits (1643), Expect = 8.7e-183
Identity = 303/476 (63.66%), Postives = 369/476 (77.52%), Query Frame = 0

Query: 1   MDHSPKPTKTHVTAARISAVIVLALVSLATVEGKKPCP--TTNYLEVPAISCRKHTALLT 60
           M+ S  P+   +       ++VLA+ S + +E +       TN +E  A++CRKHTA+LT
Sbjct: 1   MEFSINPSTLKMIRTSFCILVVLAISSFSMMEARDLASKGKTN-IEYMALNCRKHTAVLT 60

Query: 61  DFGGVGDGVTSNTHAFRQAIEHLSPLAADGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDA 120
           +FG VGDG TSNT AF++AI  L+P AADGG QLIVPPGKWLTGSFNLTSHFTLF+ K A
Sbjct: 61  EFGAVGDGKTSNTKAFKEAITKLAPKAADGGVQLIVPPGKWLTGSFNLTSHFTLFIQKGA 120

Query: 121 KILASQDESEWPLVAVLPSYGRGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGSYWW 180
            ILASQDESE+P+VA LPSYG+GRDA G  ++SLI GTNLTDVVITGNNGTI+GQG YWW
Sbjct: 121 TILASQDESEYPVVAPLPSYGQGRDAAGPTFASLISGTNLTDVVITGNNGTINGQGKYWW 180

Query: 181 DKFHKNEL-NVTRPYLIEIIYSDQIQISDLTLVNSPSWFVHPIYSRNVIIQGLTILAPID 240
            K+       +TRPY IEII+S  +QIS++T+++SP+W +HP+Y  NVI++G+TILAPID
Sbjct: 181 VKYRSGGFKGITRPYTIEIIFSQNVQISNITIIDSPAWNIHPVYCNNVIVKGVTILAPID 240

Query: 241 SPNTDGIDPDSCSNTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIRRLTCISPD 300
           SPNTDGI+PDSC+NT IEDC++VSGDDCIAVKSGWDQ+GIK GMPT+ L IRRLTCISPD
Sbjct: 241 SPNTDGINPDSCTNTLIEDCYVVSGDDCIAVKSGWDQFGIKVGMPTQQLSIRRLTCISPD 300

Query: 301 SAGIALGSEMSGGIRNVRIENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVF 360
           SAGIALGSEMSGGI++VRIE++T + TQSA+RIKTA GRGG+VKDIF R   + TMKYVF
Sbjct: 301 SAGIALGSEMSGGIKDVRIEDITLLQTQSAIRIKTAVGRGGYVKDIFARRFTMKTMKYVF 360

Query: 361 WMTGNYKSHPDEKFDPAAVPEITNINYRDVVAENVTYSARLEGISGDPFTDICISNVKIG 420
           WM+G Y  HP   FDP A+P ITNINYRD+ A+NVT  ARL+G   DPFT IC+SN+KI 
Sbjct: 361 WMSGAYNQHPASGFDPKAMPVITNINYRDMTADNVTQPARLDGFKNDPFTKICMSNIKID 420

Query: 421 LTAKPKKLQWNCTDVEGFSSDVVPPPCAPLAKAAKGGGCYFPEDQLPIEKVQLKTC 474
           L A+PKKL WNCT + G SS V P PC+ L +      C FP D++PIE V L  C
Sbjct: 421 LAAEPKKLLWNCTSISGVSSKVTPKPCSLLPEKGAPVDCAFPVDKIPIESVVLNKC 475

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038906749.17.7e-27495.62probable polygalacturonase [Benincasa hispida][more]
XP_008437531.12.1e-26392.66PREDICTED: probable polygalacturonase [Cucumis melo] >KAA0042595.1 putative poly... [more]
XP_011654644.11.8e-25992.18probable polygalacturonase [Cucumis sativus] >KGN49922.1 hypothetical protein Cs... [more]
XP_023532553.11.2e-25388.33probable polygalacturonase [Cucurbita pepo subsp. pepo][more]
XP_022996001.14.8e-25288.33probable polygalacturonase [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A7PZL31.4e-18066.01Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 ... [more]
Q9LW074.2e-3329.78Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g1572... [more]
Q7M1E71.1e-2828.75Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1[more]
Q9FY199.2e-2828.22Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1[more]
P432129.5e-2527.14Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3C4611.0e-26392.66Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A1S3AUV51.0e-26392.66probable polygalacturonase OS=Cucumis melo OX=3656 GN=LOC103482916 PE=3 SV=1[more]
A0A0A0KNF98.9e-26092.18Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G140480 PE=3 SV=1[more]
A0A6J1K3G82.3e-25288.33probable polygalacturonase OS=Cucurbita maxima OX=3661 GN=LOC111491343 PE=3 SV=1[more]
A0A6J1H0R51.4e-24986.88probable polygalacturonase OS=Cucurbita moschata OX=3662 GN=LOC111459096 PE=3 SV... [more]
Match NameE-valueIdentityDescription
AT3G48950.14.8e-18967.68Pectin lyase-like superfamily protein [more]
AT3G61490.11.0e-18364.58Pectin lyase-like superfamily protein [more]
AT3G61490.21.0e-18364.58Pectin lyase-like superfamily protein [more]
AT3G61490.31.0e-18364.58Pectin lyase-like superfamily protein [more]
AT2G23900.18.7e-18363.66Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 311..332
e-value: 750.0
score: 8.1
coord: 222..248
e-value: 1100.0
score: 6.9
coord: 249..270
e-value: 350.0
score: 10.0
coord: 283..305
e-value: 1900.0
score: 5.2
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 151..433
e-value: 1.6E-40
score: 139.2
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 39..438
e-value: 3.0E-114
score: 384.2
NoneNo IPR availablePANTHERPTHR31339:SF12ENDO-POLYGALACTURONASE-LIKE PROTEINcoord: 18..474
NoneNo IPR availablePANTHERPTHR31339PECTIN LYASE-RELATEDcoord: 18..474
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 47..475

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10008951.1HG10008951.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity