HG10008214 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10008214
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionVery-long-chain aldehyde decarbonylase GL1-3
LocationChr10: 20900026 .. 20904529 (+)
RNA-Seq ExpressionHG10008214
SyntenyHG10008214
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTGCTCCATTATCATCTTGGCCATGGGAGAATTTGGGGATGTTCAAGGTAAATTTCTGGAATATGAAATTGATGAGTGTGTTATTGATTTTGCATGTGTTTGATTTTTTCATTTAAATGAATAATGATGCAGTATTTGCTATATGGGCCATTGGTTGGAAAGGGTTTATATTCGTTATATGAAGAAGGGAACATCATAAACAATTGGTGTCTCCATATTCTTTTGATCTCTTTACTCAGAGTGGGAATTCATGTCGCTTGGAGTTCTTATAGCAACATGCTTTTCTTGACAAGAAACCGACGGATTCTTCAACAAGGAGTTGATTTCAAACAGATTGATATGGAATGGGAATGGTATTTAGTATTTACATTCAAATTACATCCCCCCCCACATCATTTTTCTTTCCAAATAATTAAAAAAAAAAAATTCTTGATAATTTCAGGGATAATTTCTTGTTACTTCAAGCTCTAATGGCTTCCATGATGATTTACCTATTCCCTTCACTTGGAAATCTTCCCCTTTGGAACACAAAAGGGTTAATTGCAGTTCAAATACTCCACGTTGGAATTGCAGAGCCATTGTTTTACATCTTCCATAGATTCTTCCACACCAACCACTATCTTTTTACTTATTACCATTCTCTTCACCATTCTTCCTCAGTCCCACAGTCTTTCACAGGTAACCCAACAATTTATTAACTTAAATCTCTTTTGGGTTCTTTTTAATTTTGATTTTTTTTATCTTAAATCTCTGAAAGTTTTGATTTTTTTTGTTTTTTTTTGTTTTGTTCTGTTTTTCCAATAAAGCTGGAAATGGGACAGTTCTGGAACATCTTGCGTTGAGTATGGTAATTGGAGCGCCAATTCTTGGAACAAGTCTTCTTGGGTATGGATCAACGGCTATGATTGTCTGTTACGTTTTGGTATTTGACTTTCTCAGATGCTTAGGGCTTTCCAATGTTGAAATTGTCCCACATCGGTTGTTTGAAGCTATCCCAATTTTTCGATATCTTCTCTACACTCCAACGTAAGTAATGATTTATTTTTATTTTTTAGCTTGTCGATGAAATTGAAAGCTCCTGTAGTTTCTTGACACCTTTGCCTCTTCTTAGTGCATAAATCGCTTTCATCATCAATGAGTATTGCTCTAAAACCACATAAAATAGAAAAAAAGGGAAAAAAAATTTTATTATTTTGTTTTAACGATTTTATCAGGTACCATACCCTTCACCATACAGAGAAGGATTCCAATTTCTGCCTCTTCATGCCTCTCTTTTATGCAATTGGAAATACCCTTCATAAAAACTCATGGGAATTACATAAGGAGAAAAGCTCAAATGCAGGTCCGTCTCTCATCTTGACTGCTAAGTTTAAAATACACCAAATATTTTTGTTTTCCAGCTTGATTCAATAATCGTCATTAATGGGTGTTTATACCTTCTCATTAAAGTAATAAAGTTCATAAAGATACGGCTCTCACGTTAATTACTTGAAAAAGGGGTCGTAAATTGCAGATTTTAGGAGTAGAAGCATTTATGACAGTGTTATTACATTATTAGAAATTTTTTTAAAGCAATTGAATACACACTTAAATATATATGGTGATAGTAAAAATGAATTAAATGAGTAGAAATTGAATAAATGCAGGAAAAAATGGGAGAGTGCCTGATTTCGTATTTCTAGCCCATGTGGTGGATGTGACTTCATCAATGCATGCGCCATTTGTATCAAGATTCTTTGCTTCACGTCCCTTTGTTATAAAATTTTCTTTATTTCCATTATGGCCCCTTGCCTTCATAACGATGCTTCTCATGTGGGGCCGCTCTAAAATCTTCCTTTTTACTTACTACCATCTTAGAGATTGGCTTCATCAAACCTGGGTTGTTCCAAGATTTGGATTTCAGGTCATTATTTTTCTCTTTTAATTATTATTATCATTCATTCTCACTTAATTAATTCCTAATTATTGTTTGTGTGGTACAGTATTTCTTGCCATTTGCAAGAGAAGGCATCAACAAACATATAGAGGATGCAATTCTGAGAGCTGACAAACTGGGGGTTAAGGTCATTAGTCTTTCTGCACTAAATAAGGTAGCTCTCTTCTCACTTTATTTCTGTTGCCAAAATGTTTTAATGTAACTCTGATCATCTCTGAATTGGGTTGGTGACGGAAATAAATATCTTTCTCTGAGGTTAATCTTTTTATCTATTGTGTGAGGACATGAATCATGTTCTATCATTTAAGACTCCTGCTCTTAATTTATTTTCTTACCTCACATACAATAAAAATATATGCTTTTTTTTTTTCTGACTCACACCTATCTTAAACATAGGGCAATGCAATAAACTTGCATACATTTGCAGTCCACTCCAACCACAACATCAAAAACCCAAAAGTATTAAAAAAAAAAAAAAAAAAAAAAAAAAGATAAATTGCAAAAACTACCTTTGAAGTATCGCGGTAGTTTGCTAGTAGCTGTAATTAGACCCTCAAACTTTCAAGTCTAAAAATTAGATCCTCAAACTTTTATAGGTATTAAAATTGGATCTCAAACTTATAATAGTTATATAAATTAGACTCCTCAATGATAAAAATCGAACCTTCAAACTTATATAATTATGGAAAATTTTCACAGATAGAAAAAATGTTAGACTATTTATAGAAAATAACAAAAAAAAAAAAAAATACTCATAGATATTGATAGACTTCTATCAACATCTATCACAACTATCTAAAAGTTTTGCTATTTCGTGTAAATAGTTTTCCTTCTTTTTTTATTTTGAAAAATTCTCCTATAATTATTACAATTTTTACCGTTATGTAAGTTTGAGAGTCCATTTTTAACAATTATATAAGTTAGAAGGTATATTGCAACTACTACTGTACTTCGGGATTGATTTTTGTAATTTACCAAAAGTTTTTTTACAAAATTGTGGTTGAACAATTTACCAGACTCAAAACGATGGGTTAAGTAACATACGATGCACCCAAATATTAAATTTTCAGAATTGAAATAATATTATTATAAGGTTTGAGCAGTGAGAAATTCAAATAAGAATATTATTATAATTTTTGTTTGTGGTTGATTTTTGGACACAGAATGAAGCTCGGAATGGTGTGAACACTTTTCGTAGAGAAGCATCCAAATCTTAGGGTAAGAGTTTTGCATGGAAATACATTAACAGCTGCTGTGATTCTCAATGAAATTCCAAAGGATGTTAAAGAAGTCTTTCTAACTGGCGCAACTTCAAAGCTTGGAAGAGCCATTGCTCTCTACCTTTGTCGAAGGAAAGTTCGAGTCCTTTTAGTCCTCTTTTTCTTTCCGAAATTATTTAATGAGGTCGTCACAAATTATTAAATTACATTCTGACAGTTTTCTCGAATAATTTCTTCTCGATTTTACAGCGATGCAATAACTATCTCATGTTACTTAGTTTGTATTCTAATATTATTAAAATATATATCACTATTAGACTTAAATTGATAGATTACGGTAAAGTTAGATTCTTTTTTATTAATGTACTTTCTTAACATTTTTTTTTTCTTTCTAAAAATTCCTTATAGATGCTTACACTTTCCACGGAAAGATTTGAGAAAATACAAAAGGAAGCACCTGCGGATTGTCAAAACTACTTGGTTCAAGTTACTAAGTACCAGCGCTGCCCAATTGCAAGGTAATTACTAAATGCTTAATTATATATATATAAAAAGAGACTCTGAATGCAGGTTTAAAAGTGATTTTAAAATCTTTAAAATTTTTTTTTTTTTTTTTTAAAATCACTCTAAAATATGCTTCTAATCACTCAAAATTAATTTAATAATAAATTTTAAATTTTTATATCATCAAAATTAATTTTGAACGATTAAAAATATGTTTCATAGTAATTTTGAAAACGATAAATATGATTTTTAACTATTTTAAAATACTGTCAAACATGCCTTAAAATGGAGGGTTGACTTGTCAAAAACTGTGACATATACTAATAATTATAATATGGATAAAATTCATGTTTTTTTTGTGACACATTAACTGATCATGTAAAAGTGTCGTCAAACTCAAACAGACTTGGATTGTTGGGAAATGGATCACTCCGAGAGAGCAAAGTTGGGCCCCAAGTGGAACTCACTTTCATCAATTTGTAGTCCCTCCCATCTTAGCTTTCAGAAGAGATTGCACATACGGCGAACTCGCAGCCATGCGTTTGCCCGACGATGTTCAAGGCCTTGGCAATTGCGAGGTATTAATTGATAATCTCTTATTCTGATACCATATTAATTTTTTATTTATATTTTTTAACGAAGAGAGGTGGTGGTTTCGTGTGATTGTTCGGGCAGTATACGATGAGCCGAGGAGTCGTGCATGCATGTCATGCGGGAGGAGTGGTGCACCACCTAGAAGGGTGGAGCCACCATGAAGTTGGGGCTTTGGATGTTGATAGAATTGACCTTGTTTGGGAGGCAGCTTTAAAACATGGCCTAAAACCAGTCTCCTCCAAATAA

mRNA sequence

ATGGTTGCTCCATTATCATCTTGGCCATGGGAGAATTTGGGGATGTTCAAGTATTTGCTATATGGGCCATTGGTTGGAAAGGGTTTATATTCGTTATATGAAGAAGGGAACATCATAAACAATTGGTGTCTCCATATTCTTTTGATCTCTTTACTCAGAGTGGGAATTCATGTCGCTTGGAGTTCTTATAGCAACATGCTTTTCTTGACAAGAAACCGACGGATTCTTCAACAAGGAGTTGATTTCAAACAGATTGATATGGAATGGGAATGGGATAATTTCTTGTTACTTCAAGCTCTAATGGCTTCCATGATGATTTACCTATTCCCTTCACTTGGAAATCTTCCCCTTTGGAACACAAAAGGGTTAATTGCAGTTCAAATACTCCACGTTGGAATTGCAGAGCCATTGTTTTACATCTTCCATAGATTCTTCCACACCAACCACTATCTTTTTACTTATTACCATTCTCTTCACCATTCTTCCTCAGTCCCACAGTCTTTCACAGCTGGAAATGGGACAGTTCTGGAACATCTTGCGTTGAGTATGGTAATTGGAGCGCCAATTCTTGGAACAAGTCTTCTTGGGTATGGATCAACGGCTATGATTGTCTGTTACGTTTTGGTATTTGACTTTCTCAGATGCTTAGGGCTTTCCAATGTTGAAATTGTCCCACATCGGTTGTTTGAAGCTATCCCAATTTTTCGATATCTTCTCTACACTCCAACGTACCATACCCTTCACCATACAGAGAAGGATTCCAATTTCTGCCTCTTCATGCCTCTCTTTTATGCAATTGGAAATACCCTTCATAAAAACTCATGGGAATTACATAAGGAGAAAAGCTCAAATGCAGGAAAAAATGGGAGAGTGCCTGATTTCGTATTTCTAGCCCATGTGGTGGATGTGACTTCATCAATGCATGCGCCATTTGTATCAAGATTCTTTGCTTCACGTCCCTTTGTTATAAAATTTTCTTTATTTCCATTATGGCCCCTTGCCTTCATAACGATGCTTCTCATGTGGGGCCGCTCTAAAATCTTCCTTTTTACTTACTACCATCTTAGAGATTGGCTTCATCAAACCTGGGTTGTTCCAAGATTTGGATTTCAGTATTTCTTGCCATTTGCAAGAGAAGGCATCAACAAACATATAGAGGATGCAATTCTGAGAGCTGACAAACTGGGGGTTAAGGTCATTAGTCTTTCTGCACTAAATAAGATGCTTACACTTTCCACGGAAAGATTTGAGAAAATACAAAAGGAAGCACCTGCGGATTGTCAAAACTACTTGGTTCAAGTTACTAAGTACCAGCGCTGCCCAATTGCAAGTGTCGTCAAACTCAAACAGACTTGGATTGTTGGGAAATGGATCACTCCGAGAGAGCAAAGTTGGGCCCCAAGTGGAACTCACTTTCATCAATTTGTAGTCCCTCCCATCTTAGCTTTCAGAAGAGATTGCACATACGGCGAACTCGCAGCCATGCGTTTGCCCGACGATGTTCAAGGCCTTGGCAATTGCGAGTATACGATGAGCCGAGGAGTCGTGCATGCATGTCATGCGGGAGGAGTGGTGCACCACCTAGAAGGGTGGAGCCACCATGAAGTTGGGGCTTTGGATGTTGATAGAATTGACCTTGTTTGGGAGGCAGCTTTAAAACATGGCCTAAAACCAGTCTCCTCCAAATAA

Coding sequence (CDS)

ATGGTTGCTCCATTATCATCTTGGCCATGGGAGAATTTGGGGATGTTCAAGTATTTGCTATATGGGCCATTGGTTGGAAAGGGTTTATATTCGTTATATGAAGAAGGGAACATCATAAACAATTGGTGTCTCCATATTCTTTTGATCTCTTTACTCAGAGTGGGAATTCATGTCGCTTGGAGTTCTTATAGCAACATGCTTTTCTTGACAAGAAACCGACGGATTCTTCAACAAGGAGTTGATTTCAAACAGATTGATATGGAATGGGAATGGGATAATTTCTTGTTACTTCAAGCTCTAATGGCTTCCATGATGATTTACCTATTCCCTTCACTTGGAAATCTTCCCCTTTGGAACACAAAAGGGTTAATTGCAGTTCAAATACTCCACGTTGGAATTGCAGAGCCATTGTTTTACATCTTCCATAGATTCTTCCACACCAACCACTATCTTTTTACTTATTACCATTCTCTTCACCATTCTTCCTCAGTCCCACAGTCTTTCACAGCTGGAAATGGGACAGTTCTGGAACATCTTGCGTTGAGTATGGTAATTGGAGCGCCAATTCTTGGAACAAGTCTTCTTGGGTATGGATCAACGGCTATGATTGTCTGTTACGTTTTGGTATTTGACTTTCTCAGATGCTTAGGGCTTTCCAATGTTGAAATTGTCCCACATCGGTTGTTTGAAGCTATCCCAATTTTTCGATATCTTCTCTACACTCCAACGTACCATACCCTTCACCATACAGAGAAGGATTCCAATTTCTGCCTCTTCATGCCTCTCTTTTATGCAATTGGAAATACCCTTCATAAAAACTCATGGGAATTACATAAGGAGAAAAGCTCAAATGCAGGAAAAAATGGGAGAGTGCCTGATTTCGTATTTCTAGCCCATGTGGTGGATGTGACTTCATCAATGCATGCGCCATTTGTATCAAGATTCTTTGCTTCACGTCCCTTTGTTATAAAATTTTCTTTATTTCCATTATGGCCCCTTGCCTTCATAACGATGCTTCTCATGTGGGGCCGCTCTAAAATCTTCCTTTTTACTTACTACCATCTTAGAGATTGGCTTCATCAAACCTGGGTTGTTCCAAGATTTGGATTTCAGTATTTCTTGCCATTTGCAAGAGAAGGCATCAACAAACATATAGAGGATGCAATTCTGAGAGCTGACAAACTGGGGGTTAAGGTCATTAGTCTTTCTGCACTAAATAAGATGCTTACACTTTCCACGGAAAGATTTGAGAAAATACAAAAGGAAGCACCTGCGGATTGTCAAAACTACTTGGTTCAAGTTACTAAGTACCAGCGCTGCCCAATTGCAAGTGTCGTCAAACTCAAACAGACTTGGATTGTTGGGAAATGGATCACTCCGAGAGAGCAAAGTTGGGCCCCAAGTGGAACTCACTTTCATCAATTTGTAGTCCCTCCCATCTTAGCTTTCAGAAGAGATTGCACATACGGCGAACTCGCAGCCATGCGTTTGCCCGACGATGTTCAAGGCCTTGGCAATTGCGAGTATACGATGAGCCGAGGAGTCGTGCATGCATGTCATGCGGGAGGAGTGGTGCACCACCTAGAAGGGTGGAGCCACCATGAAGTTGGGGCTTTGGATGTTGATAGAATTGACCTTGTTTGGGAGGCAGCTTTAAAACATGGCCTAAAACCAGTCTCCTCCAAATAA

Protein sequence

MVAPLSSWPWENLGMFKYLLYGPLVGKGLYSLYEEGNIINNWCLHILLISLLRVGIHVAWSSYSNMLFLTRNRRILQQGVDFKQIDMEWEWDNFLLLQALMASMMIYLFPSLGNLPLWNTKGLIAVQILHVGIAEPLFYIFHRFFHTNHYLFTYYHSLHHSSSVPQSFTAGNGTVLEHLALSMVIGAPILGTSLLGYGSTAMIVCYVLVFDFLRCLGLSNVEIVPHRLFEAIPIFRYLLYTPTYHTLHHTEKDSNFCLFMPLFYAIGNTLHKNSWELHKEKSSNAGKNGRVPDFVFLAHVVDVTSSMHAPFVSRFFASRPFVIKFSLFPLWPLAFITMLLMWGRSKIFLFTYYHLRDWLHQTWVVPRFGFQYFLPFAREGINKHIEDAILRADKLGVKVISLSALNKMLTLSTERFEKIQKEAPADCQNYLVQVTKYQRCPIASVVKLKQTWIVGKWITPREQSWAPSGTHFHQFVVPPILAFRRDCTYGELAAMRLPDDVQGLGNCEYTMSRGVVHACHAGGVVHHLEGWSHHEVGALDVDRIDLVWEAALKHGLKPVSSK
Homology
BLAST of HG10008214 vs. NCBI nr
Match: XP_038879572.1 (very-long-chain aldehyde decarbonylase CER3 [Benincasa hispida])

HSP 1 Score: 1041.2 bits (2691), Expect = 3.3e-300
Identity = 517/630 (82.06%), Postives = 536/630 (85.08%), Query Frame = 0

Query: 1   MVAPLSSWPWENLGMFKYLLYGPLVGKGLYSLYEEGNIINNWCLHILLISLLRVGIHVAW 60
           MVAPLSSWPW+NLGMFKYLLYGPL+ KGLYSLYEEGNIINNWCLHILLISLLRVGIH+AW
Sbjct: 1   MVAPLSSWPWDNLGMFKYLLYGPLLAKGLYSLYEEGNIINNWCLHILLISLLRVGIHIAW 60

Query: 61  SSYSNMLFLTRNRRILQQGVDFKQIDMEWEWDNFLLLQALMASMMIYLFPSLGNLPLWNT 120
           SSYSNMLFLTRNRRILQQGVDFKQIDMEWEWDNFLLLQALMASMMIYLFPSLGNLPLWNT
Sbjct: 61  SSYSNMLFLTRNRRILQQGVDFKQIDMEWEWDNFLLLQALMASMMIYLFPSLGNLPLWNT 120

Query: 121 KGLIAVQILHVGIAEPLFYIFHRFFHTNHYLFTYYHSLHHSSSVPQSFTAGNGTVLEHLA 180
           KG IAV ILHV IAEPLFYIFHRFFH+NH+LFT+YHSLHHSS VPQSFTAGN TVLEHLA
Sbjct: 121 KGFIAVLILHVVIAEPLFYIFHRFFHSNHHLFTHYHSLHHSSPVPQSFTAGNATVLEHLA 180

Query: 181 LSMVIGAPILGTSLLGYGSTAMIVCYVLVFDFLRCLGLSNVEIVPHRLFEAIPIFRYLLY 240
            SMVIGAPILGTSLLGYGSTAM+VCYVLVFDFLRCLGLSNVE+VPHRLFEAIPIFRYLLY
Sbjct: 181 WSMVIGAPILGTSLLGYGSTAMVVCYVLVFDFLRCLGLSNVEVVPHRLFEAIPIFRYLLY 240

Query: 241 TPTYHTLHHTEKDSNFCLFMPLFYAIGNTLHKNSWELHKEKSSNAGKNGRVPDFVFLAHV 300
           TPTYHTLHHTEK +NFCLFMPLF  IGNTLHKNSWELHKEKS NAGKNGRVPDFVFLAHV
Sbjct: 241 TPTYHTLHHTEKGTNFCLFMPLFDVIGNTLHKNSWELHKEKSLNAGKNGRVPDFVFLAHV 300

Query: 301 VDVTSSMHAPFVSRFFASRPFVIKFSLFPLWPLAFITMLLMWGRSKIFLFTYYHLRDWLH 360
           VDVTSSMHAPFVSRFFASRPFV K SLFP WP+AFI ML+MWGRSKIFL++YY+LRDWLH
Sbjct: 301 VDVTSSMHAPFVSRFFASRPFVTKLSLFPSWPIAFIVMLIMWGRSKIFLYSYYNLRDWLH 360

Query: 361 QTWVVPRFGFQYFLPFAREGINKHIEDAILRADKLGVKVISLSALNK------------- 420
           QTWVVPRFGFQYFLPFAREGINKHIEDAILRADKLGVKVISL+ALNK             
Sbjct: 361 QTWVVPRFGFQYFLPFAREGINKHIEDAILRADKLGVKVISLAALNKNEALNGGGTLFVE 420

Query: 421 --------------------------------------------------------MLTL 480
                                                                   MLTL
Sbjct: 421 NHPNLRVRVVHGNTLTAAVILNEIPKEVKEVFLTGATSKLGRAIALYLCRRKVRVLMLTL 480

Query: 481 STERFEKIQKEAPADCQNYLVQVTKYQRCPIASVVKLKQTWIVGKWITPREQSWAPSGTH 540
           STERFEKIQKEAPADCQNYLVQVTKYQ        +  +TWIVGKWITPREQSWAPSGTH
Sbjct: 481 STERFEKIQKEAPADCQNYLVQVTKYQ------AARNCKTWIVGKWITPREQSWAPSGTH 540

Query: 541 FHQFVVPPILAFRRDCTYGELAAMRLPDDVQGLGNCEYTMSRGVVHACHAGGVVHHLEGW 562
           FHQFVVPPILAFR+DCTYGELAAMRLPDDVQGLGNCEYTMSRGVVHACHAGGVVHHLEGW
Sbjct: 541 FHQFVVPPILAFRKDCTYGELAAMRLPDDVQGLGNCEYTMSRGVVHACHAGGVVHHLEGW 600

BLAST of HG10008214 vs. NCBI nr
Match: XP_004149879.1 (very-long-chain aldehyde decarbonylase CER3 [Cucumis sativus] >KGN65271.1 hypothetical protein Csa_019744 [Cucumis sativus])

HSP 1 Score: 1009.2 bits (2608), Expect = 1.4e-290
Identity = 499/631 (79.08%), Postives = 527/631 (83.52%), Query Frame = 0

Query: 1   MVAPLSSWPWENLGMFKYLLYGPLVGKGLYSLYEEGNIINNWCLHILLISLLRVGIHVAW 60
           MVAPL+SWPWENLGMFKYLLYGPL+  GLY+LYEEGNII+NWCLHILLISLLRVGIHV W
Sbjct: 1   MVAPLASWPWENLGMFKYLLYGPLLANGLYTLYEEGNIIHNWCLHILLISLLRVGIHVVW 60

Query: 61  SSYSNMLFLTRNRRILQQGVDFKQIDMEWEWDNFLLLQALMASMMIYLFPSLGNLPLWNT 120
           SSYSNMLFLTRNRRILQQGVDFKQIDMEWEWDNFLLLQALM SMM+YLFPSLGNLPLWN 
Sbjct: 61  SSYSNMLFLTRNRRILQQGVDFKQIDMEWEWDNFLLLQALMTSMMVYLFPSLGNLPLWNP 120

Query: 121 KGLIAVQILHVGIAEPLFYIFHRFFHTNHYLFTYYHSLHHSSSVPQSFTAGNGTVLEHLA 180
           KGLIAV ILH+ IAEPLFY FHR FH+NHYLFT+YHSLHHSSSVPQSFTAGNGTVLEHLA
Sbjct: 121 KGLIAVLILHIVIAEPLFYFFHRLFHSNHYLFTHYHSLHHSSSVPQSFTAGNGTVLEHLA 180

Query: 181 LSMVIGAPILGTSLLGYGSTAMIVCYVLVFDFLRCLGLSNVEIVPHRLFEAIPIFRYLLY 240
            S+VIGAPI+GTSLLGYGSTA   CYVLVFDFLRCLGLSNVEIV HRLF+AIP+ RYLLY
Sbjct: 181 WSIVIGAPIVGTSLLGYGSTATFACYVLVFDFLRCLGLSNVEIVSHRLFDAIPVLRYLLY 240

Query: 241 TPTYHTLHHTEKDSNFCLFMPLFYAIGNTLHKNSWELHKEKSSNAGKNGRVPDFVFLAHV 300
           TPTYHTLHHTEK++NFCLFMPLF AIGNTLHK SW+LHK+ S NAGKNGRVPDFVFLAHV
Sbjct: 241 TPTYHTLHHTEKETNFCLFMPLFDAIGNTLHKCSWKLHKQNSLNAGKNGRVPDFVFLAHV 300

Query: 301 VDVTSSMHAPFVSRFFASRPFVIKFSLFPLWPLAFITMLLMWGRSKIFLFTYYHLRDWLH 360
           VDVTSSMHAPFVSRFFASRPFV K SLFP WP AFI ML+MWGRSKIFL++YY+LR+WLH
Sbjct: 301 VDVTSSMHAPFVSRFFASRPFVTKLSLFPSWPAAFIVMLIMWGRSKIFLYSYYNLRNWLH 360

Query: 361 QTWVVPRFGFQYFLPFAREGINKHIEDAILRADKLGVKVISLSALNK------------- 420
           QTWVVPRFGFQYFLPFAREGINKHIEDAILRADKLGVKVISL+ALNK             
Sbjct: 361 QTWVVPRFGFQYFLPFAREGINKHIEDAILRADKLGVKVISLAALNKNEALNGGGTLFVE 420

Query: 421 --------------------------------------------------------MLTL 480
                                                                   MLTL
Sbjct: 421 KHPNLRVRVVHGNTLTAAVILNEIPKDVKEVFLTGATSKLGRAIALYLCRRKVRVLMLTL 480

Query: 481 STERFEKIQKEAPADCQNYLVQVTKYQRCPIASVVKLKQTWIVGKWITPREQSWAPSGTH 540
           STERFEKIQKEAP DCQNYLVQVTKYQ        +  +TWIVGKWITPREQSWAPSGTH
Sbjct: 481 STERFEKIQKEAPVDCQNYLVQVTKYQ------AARNCKTWIVGKWITPREQSWAPSGTH 540

Query: 541 FHQFVVPPILAFRRDCTYGELAAMRLPDDVQGLGNCEYTMSRGVVHACHAGGVVHHLEGW 563
           FHQFVVPPILAFRRDCTYG+LAAMRLP+DVQGLGNCEYTMSRGVVHACHAGGVVHHLEGW
Sbjct: 541 FHQFVVPPILAFRRDCTYGDLAAMRLPEDVQGLGNCEYTMSRGVVHACHAGGVVHHLEGW 600

BLAST of HG10008214 vs. NCBI nr
Match: XP_023515806.1 (protein ECERIFERUM 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 993.4 bits (2567), Expect = 7.8e-286
Identity = 489/631 (77.50%), Postives = 524/631 (83.04%), Query Frame = 0

Query: 1   MVAPLSSWPWENLGMFKYLLYGPLVGKGLYSLYEEGNIINNWCLHILLISLLRVGIHVAW 60
           +VAPLSSWPWENLG  KYLLYGPL+  G YSLY++GNI+ NWCLHILL+SLLR+GIHVAW
Sbjct: 3   VVAPLSSWPWENLGSLKYLLYGPLLANGFYSLYQDGNILQNWCLHILLLSLLRMGIHVAW 62

Query: 61  SSYSNMLFLTRNRRILQQGVDFKQIDMEWEWDNFLLLQALMASMMIYLFPSLGNLPLWNT 120
           SSYSNMLFLTRNRRI+QQGVDFKQIDMEWEWDNFL+LQ+LMASMM+YLFP LGNLPLWNT
Sbjct: 63  SSYSNMLFLTRNRRIIQQGVDFKQIDMEWEWDNFLILQSLMASMMVYLFPWLGNLPLWNT 122

Query: 121 KGLIAVQILHVGIAEPLFYIFHRFFHTNHYLFTYYHSLHHSSSVPQSFTAGNGTVLEHLA 180
           KGLIA+ ILHVGIAEPLFY+FHRFFHT HYLFT+YHSLHHSS VPQSFTAGN T LEHLA
Sbjct: 123 KGLIAILILHVGIAEPLFYMFHRFFHT-HYLFTHYHSLHHSSPVPQSFTAGNATFLEHLA 182

Query: 181 LSMVIGAPILGTSLLGYGSTAMIVCYVLVFDFLRCLGLSNVEIVPHRLFEAIPIFRYLLY 240
            S+VIGAPILGTSLLGYGST MI CYVLVFDFLRCLGLSNVEIVPHRLFEA+PI RYLLY
Sbjct: 183 WSLVIGAPILGTSLLGYGSTIMIFCYVLVFDFLRCLGLSNVEIVPHRLFEAVPILRYLLY 242

Query: 241 TPTYHTLHHTEKDSNFCLFMPLFYAIGNTLHKNSWELHKEKSSNAGKNGRVPDFVFLAHV 300
           TPTYHTLH TEK SNFCLFMPLF AIGNT+HKNSWELHKE SS AGKNG+VPDFVFLAHV
Sbjct: 243 TPTYHTLHRTEKGSNFCLFMPLFDAIGNTVHKNSWELHKEMSSKAGKNGKVPDFVFLAHV 302

Query: 301 VDVTSSMHAPFVSRFFASRPFVIKFSLFPLWPLAFITMLLMWGRSKIFLFTYYHLRDWLH 360
           VD+TSSMHAPFVSRFFASRPFV K SLFPLWP+AFI ML+MWGRSK FL+++Y+LRDWLH
Sbjct: 303 VDITSSMHAPFVSRFFASRPFVTKLSLFPLWPIAFIVMLVMWGRSKPFLYSFYNLRDWLH 362

Query: 361 QTWVVPRFGFQYFLPFAREGINKHIEDAILRADKLGVKVISLSALNK------------- 420
           QTW VPRFGFQYFLPFAREGIN HIE+AILRADKLGVKVISL+ALNK             
Sbjct: 363 QTWAVPRFGFQYFLPFAREGINNHIEEAILRADKLGVKVISLAALNKNEALNGGGTLFVE 422

Query: 421 --------------------------------------------------------MLTL 480
                                                                   MLTL
Sbjct: 423 KHPDLRVRVVHGNTLTAAVILNEIPKDVKEVFLTGATSKLGRAIALYLCRRKVRVLMLTL 482

Query: 481 STERFEKIQKEAPADCQNYLVQVTKYQRCPIASVVKLKQTWIVGKWITPREQSWAPSGTH 540
           +TERFEKIQKEAP +CQ+YLVQVTKYQ        +  +TWIVGKWITPREQSWAPSGTH
Sbjct: 483 ATERFEKIQKEAPTECQSYLVQVTKYQ------AARNCKTWIVGKWITPREQSWAPSGTH 542

Query: 541 FHQFVVPPILAFRRDCTYGELAAMRLPDDVQGLGNCEYTMSRGVVHACHAGGVVHHLEGW 563
           FHQFVVPPILAFRRDCTYG+LAAMRLPDDVQGLGNCEYTMSRGVVHACHAGGVVHHLEGW
Sbjct: 543 FHQFVVPPILAFRRDCTYGDLAAMRLPDDVQGLGNCEYTMSRGVVHACHAGGVVHHLEGW 602

BLAST of HG10008214 vs. NCBI nr
Match: KAG6589741.1 (Very-long-chain aldehyde decarbonylase CER3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 989.9 bits (2558), Expect = 8.7e-285
Identity = 488/631 (77.34%), Postives = 522/631 (82.73%), Query Frame = 0

Query: 1   MVAPLSSWPWENLGMFKYLLYGPLVGKGLYSLYEEGNIINNWCLHILLISLLRVGIHVAW 60
           +VAPLSSWPWENLG  KYLLYGPL+  G YSLY++GNI+ NWCLHILL+S+LR+GIHVAW
Sbjct: 3   VVAPLSSWPWENLGSLKYLLYGPLLANGFYSLYQDGNILQNWCLHILLLSILRMGIHVAW 62

Query: 61  SSYSNMLFLTRNRRILQQGVDFKQIDMEWEWDNFLLLQALMASMMIYLFPSLGNLPLWNT 120
           SSYSNMLFLTRNRRILQQGVDFKQIDMEWEWDNFL+L +LMASMM+YLFP LGNLPLWNT
Sbjct: 63  SSYSNMLFLTRNRRILQQGVDFKQIDMEWEWDNFLILHSLMASMMVYLFPWLGNLPLWNT 122

Query: 121 KGLIAVQILHVGIAEPLFYIFHRFFHTNHYLFTYYHSLHHSSSVPQSFTAGNGTVLEHLA 180
           KGLIA+ ILHVGIAEPLFY+FHRFFHT HYLFT+YHSLHHSS VPQSFTAGN T LEHLA
Sbjct: 123 KGLIAILILHVGIAEPLFYVFHRFFHT-HYLFTHYHSLHHSSPVPQSFTAGNATFLEHLA 182

Query: 181 LSMVIGAPILGTSLLGYGSTAMIVCYVLVFDFLRCLGLSNVEIVPHRLFEAIPIFRYLLY 240
            S+VIGAPILGTSLLGYGST MI CYVLVFDFLRCLGLSNVEIVPHRLFEA+PI RYLLY
Sbjct: 183 WSLVIGAPILGTSLLGYGSTTMIFCYVLVFDFLRCLGLSNVEIVPHRLFEAVPILRYLLY 242

Query: 241 TPTYHTLHHTEKDSNFCLFMPLFYAIGNTLHKNSWELHKEKSSNAGKNGRVPDFVFLAHV 300
           TPTYHTLH TEK SNFCLFMPLF AIGNT+HKNSWELHKE SS AGKNG+VPDFVFLAHV
Sbjct: 243 TPTYHTLHRTEKGSNFCLFMPLFDAIGNTVHKNSWELHKEMSSTAGKNGKVPDFVFLAHV 302

Query: 301 VDVTSSMHAPFVSRFFASRPFVIKFSLFPLWPLAFITMLLMWGRSKIFLFTYYHLRDWLH 360
           VD+TSSMHAPFVSRFFASRPFV K SLFPLWP+AFI ML+MWGRSK FL ++Y+LRDWLH
Sbjct: 303 VDITSSMHAPFVSRFFASRPFVTKLSLFPLWPIAFIVMLVMWGRSKPFLHSFYNLRDWLH 362

Query: 361 QTWVVPRFGFQYFLPFAREGINKHIEDAILRADKLGVKVISLSALNK------------- 420
           QTW VPRFGFQYFLPFAREGIN HIE+AILRADKLGVKVISL+ALNK             
Sbjct: 363 QTWAVPRFGFQYFLPFAREGINNHIEEAILRADKLGVKVISLAALNKNEALNGGGTLFVE 422

Query: 421 --------------------------------------------------------MLTL 480
                                                                   MLTL
Sbjct: 423 KHPNLRVRVVHGNTLTAAVILNEIPKDVKEVFLTGATSKLGRAIALYLCRRKVRVLMLTL 482

Query: 481 STERFEKIQKEAPADCQNYLVQVTKYQRCPIASVVKLKQTWIVGKWITPREQSWAPSGTH 540
           +TERFEKIQKEAP +CQ+YLVQVTKYQ        +  +TWIVGKWITPREQSWAPSGTH
Sbjct: 483 ATERFEKIQKEAPTECQSYLVQVTKYQ------AARNCKTWIVGKWITPREQSWAPSGTH 542

Query: 541 FHQFVVPPILAFRRDCTYGELAAMRLPDDVQGLGNCEYTMSRGVVHACHAGGVVHHLEGW 563
           FHQFVVPPILAFRRDCTYG+LAAMRLPDDV+GLGNCEYTMSRGVVHACHAGGVVHHLEGW
Sbjct: 543 FHQFVVPPILAFRRDCTYGDLAAMRLPDDVKGLGNCEYTMSRGVVHACHAGGVVHHLEGW 602

BLAST of HG10008214 vs. NCBI nr
Match: XP_022921868.1 (protein ECERIFERUM 3-like isoform X1 [Cucurbita moschata] >KAG7023416.1 Protein ECERIFERUM 3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 989.9 bits (2558), Expect = 8.7e-285
Identity = 488/631 (77.34%), Postives = 522/631 (82.73%), Query Frame = 0

Query: 1   MVAPLSSWPWENLGMFKYLLYGPLVGKGLYSLYEEGNIINNWCLHILLISLLRVGIHVAW 60
           +VAPLSSWPWENLG  KYLLYGPL+  G YSLY++GNI+ NWCLHILL+S+LR+GIHVAW
Sbjct: 3   VVAPLSSWPWENLGSLKYLLYGPLLANGFYSLYQDGNILQNWCLHILLLSILRMGIHVAW 62

Query: 61  SSYSNMLFLTRNRRILQQGVDFKQIDMEWEWDNFLLLQALMASMMIYLFPSLGNLPLWNT 120
           SSYSNMLFLTRNRRILQQGVDFKQIDMEWEWDNFL+L +LMASMM+YLFP LGNLPLWNT
Sbjct: 63  SSYSNMLFLTRNRRILQQGVDFKQIDMEWEWDNFLILHSLMASMMVYLFPWLGNLPLWNT 122

Query: 121 KGLIAVQILHVGIAEPLFYIFHRFFHTNHYLFTYYHSLHHSSSVPQSFTAGNGTVLEHLA 180
           KGLIA+ ILHVGIAEPLFY+FHRFFHT HYLFT+YHSLHHSS VPQSFTAGN T LEHLA
Sbjct: 123 KGLIAILILHVGIAEPLFYVFHRFFHT-HYLFTHYHSLHHSSPVPQSFTAGNATFLEHLA 182

Query: 181 LSMVIGAPILGTSLLGYGSTAMIVCYVLVFDFLRCLGLSNVEIVPHRLFEAIPIFRYLLY 240
            S+VIGAPILGTSLLGYGST MI CYVLVFDFLRCLGLSNVEIVPHRLFEA+PI RYLLY
Sbjct: 183 WSLVIGAPILGTSLLGYGSTTMIFCYVLVFDFLRCLGLSNVEIVPHRLFEAVPILRYLLY 242

Query: 241 TPTYHTLHHTEKDSNFCLFMPLFYAIGNTLHKNSWELHKEKSSNAGKNGRVPDFVFLAHV 300
           TPTYHTLH TEK SNFCLFMPLF AIGNT+HKNSWELHKE SS AGKNG+VPDFVFLAHV
Sbjct: 243 TPTYHTLHRTEKGSNFCLFMPLFDAIGNTVHKNSWELHKEMSSTAGKNGKVPDFVFLAHV 302

Query: 301 VDVTSSMHAPFVSRFFASRPFVIKFSLFPLWPLAFITMLLMWGRSKIFLFTYYHLRDWLH 360
           VD+TSSMHAPFVSRFFASRPFV K SLFPLWP+AFI ML+MWGRSK FL+++Y+LRDWLH
Sbjct: 303 VDITSSMHAPFVSRFFASRPFVTKLSLFPLWPIAFIVMLVMWGRSKPFLYSFYNLRDWLH 362

Query: 361 QTWVVPRFGFQYFLPFAREGINKHIEDAILRADKLGVKVISLSALNK------------- 420
           QTW VPRFGFQYFLPFAREGIN HIE+AILRADKLGVKVISL+ALNK             
Sbjct: 363 QTWAVPRFGFQYFLPFAREGINNHIEEAILRADKLGVKVISLAALNKNEALNGGGTLFVE 422

Query: 421 --------------------------------------------------------MLTL 480
                                                                   MLTL
Sbjct: 423 KHPNLRVRVVHGNTLTAAVILNEIPKDVKEVFLTGATSKLGRAIALYLCRRKVRVLMLTL 482

Query: 481 STERFEKIQKEAPADCQNYLVQVTKYQRCPIASVVKLKQTWIVGKWITPREQSWAPSGTH 540
           +TERFEKIQKEAP +CQ+YLVQVTKYQ        +  +TWIVGKWITPREQSWAPSGTH
Sbjct: 483 ATERFEKIQKEAPTECQSYLVQVTKYQ------AARNCKTWIVGKWITPREQSWAPSGTH 542

Query: 541 FHQFVVPPILAFRRDCTYGELAAMRLPDDVQGLGNCEYTMSRGVVHACHAGGVVHHLEGW 563
           FHQFVVPPILAFRRDCTYG+LAAMRLPDDVQGLG CEYTMSRGVVHACHAGGVVHHLEGW
Sbjct: 543 FHQFVVPPILAFRRDCTYGDLAAMRLPDDVQGLGYCEYTMSRGVVHACHAGGVVHHLEGW 602

BLAST of HG10008214 vs. ExPASy Swiss-Prot
Match: Q8H1Z0 (Very-long-chain aldehyde decarbonylase CER3 OS=Arabidopsis thaliana OX=3702 GN=CER3 PE=1 SV=1)

HSP 1 Score: 710.7 bits (1833), Expect = 1.3e-203
Identity = 362/638 (56.74%), Postives = 435/638 (68.18%), Query Frame = 0

Query: 1   MVAPLSSWPWENLGMFKYLLYGPLVGKGLYS-LYEEGNIINNWCLHILLISLLRVGIHVA 60
           MVA LS+WPWEN G  KYLLY PL  + +YS +YEE      WC+HIL+I  L+  +H  
Sbjct: 1   MVAFLSAWPWENFGNLKYLLYAPLAAQVVYSWVYEEDISKVLWCIHILIICGLKALVHEL 60

Query: 61  WSSYSNMLFLTRNRRILQQGVDFKQIDMEWEWDNFLLLQALMASMMIYLFPSL----GNL 120
           WS ++NMLF+TR  RI  +G+DFKQID EW WDN+++LQA++ S++ Y+ P L     +L
Sbjct: 61  WSVFNNMLFVTRTLRINPKGIDFKQIDHEWHWDNYIILQAIIVSLICYMSPPLMMMINSL 120

Query: 121 PLWNTKGLIAVQILHVGIAEPLFYIFHRFFHTNHYLFTYYHSLHHSSSVPQSFTAGNGTV 180
           PLWNTKGLIA+ +LHV  +EPL+Y  HR FH N+Y FT+YHS HHSS VP   TAGN T+
Sbjct: 121 PLWNTKGLIALIVLHVTFSEPLYYFLHRSFHRNNYFFTHYHSFHHSSPVPHPMTAGNATL 180

Query: 181 LEHLALSMVIGAPILGTSLLGYGSTAMIVCYVLVFDFLRCLGLSNVEIVPHRLFEAIPIF 240
           LE++ L +V G P++G  L G GS + I  Y ++FDF+RCLG  NVEI  H+LFE +P+ 
Sbjct: 181 LENIILCVVAGVPLIGCCLFGVGSLSAIYGYAVMFDFMRCLGHCNVEIFSHKLFEILPVL 240

Query: 241 RYLLYTPTYHTLHHTEKDSNFCLFMPLFYAIGNTLHKNSWELHKEKSSNAGKNGRVPDFV 300
           RYL+YTPTYH+LHH E  +NFCLFMPLF  +G+T + NSWEL K+   +AG+  RVP+FV
Sbjct: 241 RYLIYTPTYHSLHHQEMGTNFCLFMPLFDVLGDTQNPNSWELQKKIRLSAGERKRVPEFV 300

Query: 301 FLAHVVDVTSSMHAPFVSRFFASRPFVIKFSLFPLWPLAFITMLLMWGRSKIFLFTYYHL 360
           FLAH VDV S+MHAPFV R FAS P+  +  L P+WP  F  ML MW  SK FLF++Y L
Sbjct: 301 FLAHGVDVMSAMHAPFVFRSFASMPYTTRIFLLPMWPFTFCVMLGMWAWSKTFLFSFYTL 360

Query: 361 RDWLHQTWVVPRFGFQYFLPFAREGINKHIEDAILRADKLGVKVISLSALNK-------- 420
           R+ L QTW VPRFGFQYFLPFA +GIN  IE AILRADK+GVKVISL+ALNK        
Sbjct: 361 RNNLCQTWGVPRFGFQYFLPFATKGINDQIEAAILRADKIGVKVISLAALNKNEALNGGG 420

Query: 421 ------------------------------------------------------------ 480
                                                                       
Sbjct: 421 TLFVNKHPDLRVRVVHGNTLTAAVILYEIPKDVNEVFLTGATSKLGRAIALYLCRRGVRV 480

Query: 481 -MLTLSTERFEKIQKEAPADCQNYLVQVTKY---QRCPIASVVKLKQTWIVGKWITPREQ 540
            MLTLS ERF+KIQKEAP + QN LVQVTKY   Q C         +TWIVGKW+TPREQ
Sbjct: 481 LMLTLSMERFQKIQKEAPVEFQNNLVQVTKYNAAQHC---------KTWIVGKWLTPREQ 540

Query: 541 SWAPSGTHFHQFVVPPILAFRRDCTYGELAAMRLPDDVQGLGNCEYTMSRGVVHACHAGG 562
           SWAP+GTHFHQFVVPPIL FRR+CTYG+LAAM+LP DV+GLG CEYTM RGVVHACHAGG
Sbjct: 541 SWAPAGTHFHQFVVPPILKFRRNCTYGDLAAMKLPKDVEGLGTCEYTMERGVVHACHAGG 600

BLAST of HG10008214 vs. ExPASy Swiss-Prot
Match: A2Z1F5 (Very-long-chain aldehyde decarbonylase GL1-1 OS=Oryza sativa subsp. indica OX=39946 GN=GL1-1 PE=3 SV=1)

HSP 1 Score: 662.9 bits (1709), Expect = 3.2e-189
Identity = 337/627 (53.75%), Postives = 413/627 (65.87%), Query Frame = 0

Query: 5   LSSWPWENLGMFKYLLYGPLVGKGLYSLYEEGNIINNWCLHILLISLLRVGIHVAWSSYS 64
           LSSWPW+NLG +KY+LY PLVGK +     E    ++W L +L++  +R   +  WSS+S
Sbjct: 6   LSSWPWDNLGAYKYVLYAPLVGKAVAGRAWERASPDHWLLLLLVLFGVRALTYQLWSSFS 65

Query: 65  NMLFLTRNRRILQQGVDFKQIDMEWEWDNFLLLQALMASMMIYLFPSLGNLPLWNTKGLI 124
           NMLF TR RRI++ GVDF QID EW+WDNFL+LQ  MA+   Y FPSL +LPLW+ +GL 
Sbjct: 66  NMLFATRRRRIVRDGVDFGQIDREWDWDNFLILQVHMAAAAFYAFPSLRHLPLWDARGLA 125

Query: 125 AVQILHVGIAEPLFYIFHRFFHTNHYLFTYYHSLHHSSSVPQSFTAGNGTVLEHLALSMV 184
              +LHV   EPLFY  HR FH  H LF+ YH  HHS+ VPQ FTAG  T LE L L  +
Sbjct: 126 VAALLHVAATEPLFYAAHRAFHRGH-LFSCYHLQHHSAKVPQPFTAGFATPLEQLVLGAL 185

Query: 185 IGAPILGTSLLGYGSTAMIVCYVLVFDFLRCLGLSNVEIVPHRLFEAIPIFRYLLYTPTY 244
           +  P+      G+GS A+   YVL FD LR +G  NVE+ P  LF+++P+ +YL+YTPTY
Sbjct: 186 MAVPLAAACAAGHGSVALAFAYVLGFDNLRAMGHCNVEVFPGGLFQSLPVLKYLIYTPTY 245

Query: 245 HTLHHTEKDSNFCLFMPLFYAIGNTLHKNSWELHKEKSSNAGKNGRVPDFVFLAHVVDVT 304
           HT+HHT++D+NFCLFMPLF  IG TL   SWE+ K+ S+   +   VP+FVFLAHVVDV 
Sbjct: 246 HTIHHTKEDANFCLFMPLFDLIGGTLDAQSWEMQKKTSAGVDE---VPEFVFLAHVVDVM 305

Query: 305 SSMHAPFVSRFFASRPFVIKFSLFPLWPLAFITMLLMWGRSKIFLFTYYHLRDWLHQTWV 364
            S+H PFV R FAS PF ++  L P+WP AF+ ML+MW  SK F+ + Y LR  LHQ W 
Sbjct: 306 QSLHVPFVLRTFASTPFSVQPFLLPMWPFAFLVMLMMWAWSKTFVISCYRLRGRLHQMWA 365

Query: 365 VPRFGFQYFLPFAREGINKHIEDAILRADKLGVKVISLSALNK----------------- 424
           VPR+GF YFLPFA++GIN  IE AILRADK+G KV+SL+ALNK                 
Sbjct: 366 VPRYGFHYFLPFAKDGINNQIELAILRADKMGAKVVSLAALNKNEALNGGGTLFVNKHPG 425

Query: 425 ----------------------------------------------------MLTLSTER 484
                                                               M+TLSTER
Sbjct: 426 LRVRVVHGNTLTAAVILNEIPQGTTEVFMTGATSKLGRAIALYLCRKKVRVMMMTLSTER 485

Query: 485 FEKIQKEAPADCQNYLVQVTKY---QRCPIASVVKLKQTWIVGKWITPREQSWAPSGTHF 544
           F+KIQ+EA  + Q YLVQVTKY   Q C         +TWIVGKW++PREQ WAP GTHF
Sbjct: 486 FQKIQREATPEHQQYLVQVTKYRSAQHC---------KTWIVGKWLSPREQRWAPPGTHF 545

Query: 545 HQFVVPPILAFRRDCTYGELAAMRLPDDVQGLGNCEYTMSRGVVHACHAGGVVHHLEGWS 560
           HQFVVPPI+ FRRDCTYG+LAAMRLP DVQGLG CEY++ RGVVHACHAGGVVH LEG++
Sbjct: 546 HQFVVPPIIGFRRDCTYGKLAAMRLPKDVQGLGACEYSLERGVVHACHAGGVVHFLEGYT 605

BLAST of HG10008214 vs. ExPASy Swiss-Prot
Match: Q69PA8 (Very-long-chain aldehyde decarbonylase GL1-1 OS=Oryza sativa subsp. japonica OX=39947 GN=GL1-1 PE=2 SV=1)

HSP 1 Score: 662.9 bits (1709), Expect = 3.2e-189
Identity = 337/627 (53.75%), Postives = 413/627 (65.87%), Query Frame = 0

Query: 5   LSSWPWENLGMFKYLLYGPLVGKGLYSLYEEGNIINNWCLHILLISLLRVGIHVAWSSYS 64
           LSSWPW+NLG +KY+LY PLVGK +     E    ++W L +L++  +R   +  WSS+S
Sbjct: 6   LSSWPWDNLGAYKYVLYAPLVGKAVAGRAWERASPDHWLLLLLVLFGVRALTYQLWSSFS 65

Query: 65  NMLFLTRNRRILQQGVDFKQIDMEWEWDNFLLLQALMASMMIYLFPSLGNLPLWNTKGLI 124
           NMLF TR RRI++ GVDF QID EW+WDNFL+LQ  MA+   Y FPSL +LPLW+ +GL 
Sbjct: 66  NMLFATRRRRIVRDGVDFGQIDREWDWDNFLILQVHMAAAAFYAFPSLRHLPLWDARGLA 125

Query: 125 AVQILHVGIAEPLFYIFHRFFHTNHYLFTYYHSLHHSSSVPQSFTAGNGTVLEHLALSMV 184
              +LHV   EPLFY  HR FH  H LF+ YH  HHS+ VPQ FTAG  T LE L L  +
Sbjct: 126 VAALLHVAATEPLFYAAHRAFHRGH-LFSCYHLQHHSAKVPQPFTAGFATPLEQLVLGAL 185

Query: 185 IGAPILGTSLLGYGSTAMIVCYVLVFDFLRCLGLSNVEIVPHRLFEAIPIFRYLLYTPTY 244
           +  P+      G+GS A+   YVL FD LR +G  NVE+ P  LF+++P+ +YL+YTPTY
Sbjct: 186 MAVPLAAACAAGHGSVALAFAYVLGFDNLRAMGHCNVEVFPGGLFQSLPVLKYLIYTPTY 245

Query: 245 HTLHHTEKDSNFCLFMPLFYAIGNTLHKNSWELHKEKSSNAGKNGRVPDFVFLAHVVDVT 304
           HT+HHT++D+NFCLFMPLF  IG TL   SWE+ K+ S+   +   VP+FVFLAHVVDV 
Sbjct: 246 HTIHHTKEDANFCLFMPLFDLIGGTLDAQSWEMQKKTSAGVDE---VPEFVFLAHVVDVM 305

Query: 305 SSMHAPFVSRFFASRPFVIKFSLFPLWPLAFITMLLMWGRSKIFLFTYYHLRDWLHQTWV 364
            S+H PFV R FAS PF ++  L P+WP AF+ ML+MW  SK F+ + Y LR  LHQ W 
Sbjct: 306 QSLHVPFVLRTFASTPFSVQPFLLPMWPFAFLVMLMMWAWSKTFVISCYRLRGRLHQMWA 365

Query: 365 VPRFGFQYFLPFAREGINKHIEDAILRADKLGVKVISLSALNK----------------- 424
           VPR+GF YFLPFA++GIN  IE AILRADK+G KV+SL+ALNK                 
Sbjct: 366 VPRYGFHYFLPFAKDGINNQIELAILRADKMGAKVVSLAALNKNEALNGGGTLFVNKHPG 425

Query: 425 ----------------------------------------------------MLTLSTER 484
                                                               M+TLSTER
Sbjct: 426 LRVRVVHGNTLTAAVILNEIPQGTTEVFMTGATSKLGRAIALYLCRKKVRVMMMTLSTER 485

Query: 485 FEKIQKEAPADCQNYLVQVTKY---QRCPIASVVKLKQTWIVGKWITPREQSWAPSGTHF 544
           F+KIQ+EA  + Q YLVQVTKY   Q C         +TWIVGKW++PREQ WAP GTHF
Sbjct: 486 FQKIQREATPEHQQYLVQVTKYRSAQHC---------KTWIVGKWLSPREQRWAPPGTHF 545

Query: 545 HQFVVPPILAFRRDCTYGELAAMRLPDDVQGLGNCEYTMSRGVVHACHAGGVVHHLEGWS 560
           HQFVVPPI+ FRRDCTYG+LAAMRLP DVQGLG CEY++ RGVVHACHAGGVVH LEG++
Sbjct: 546 HQFVVPPIIGFRRDCTYGKLAAMRLPKDVQGLGACEYSLERGVVHACHAGGVVHFLEGYT 605

BLAST of HG10008214 vs. ExPASy Swiss-Prot
Match: Q67WQ7 (Very-long-chain aldehyde decarbonylase GL1-3 OS=Oryza sativa subsp. japonica OX=39947 GN=GL1-3 PE=2 SV=2)

HSP 1 Score: 642.9 bits (1657), Expect = 3.4e-183
Identity = 331/631 (52.46%), Postives = 413/631 (65.45%), Query Frame = 0

Query: 1   MVAPLSSWPWENLGMFKYLLYGPLVGKGLYSLYEEGNII--NNWCLHILLISLLRVGIHV 60
           M +PLSSWPW  LG +KYLLYGP+VGK +    E+G +    +WCLH++L+  LR     
Sbjct: 5   MASPLSSWPWAFLGSYKYLLYGPVVGKVVQEWREQGRLPLGTSWCLHLILLLALR----- 64

Query: 61  AWSSYSNMLFLTRNRRILQQGVDFKQIDMEWEWDNFLLLQALMASMMI--YLFPSLGNLP 120
                  MLF TR RR++  GVDF+QID EW+WDN +++Q L+A++++   +FP+  +L 
Sbjct: 65  ----SLTMLFFTRRRRVVDDGVDFRQIDTEWDWDNMVIMQTLIAAVLVTSRVFPATSDLS 124

Query: 121 LWNTKGLIAVQILHVGIAEPLFYIFHRFFHTNHYLFTYYHSLHHSSSVPQSFTAGNGTVL 180
            W+ +G     +LHV ++EP FY  HR  H    LF+ YHSLHHS    Q+ TAG  T L
Sbjct: 125 AWDLRGWAIAVVLHVAVSEPAFYWAHRALHLGP-LFSRYHSLHHSFQATQALTAGFVTPL 184

Query: 181 EHLALSMVIGAPILGTSLLGYGSTAMIVCYVLVFDFLRCLGLSNVEIVPHRLFEAIPIFR 240
           E L L++V  AP+ G  + G+GS +++  ++L+FD+LR +G SNVE++ H+ F+  P  R
Sbjct: 185 ESLILTLVAWAPLAGAFMAGHGSVSLVYGHILLFDYLRSMGYSNVEVISHKTFQDFPFLR 244

Query: 241 YLLYTPTYHTLHHTEKDSNFCLFMPLFYAIGNTLHKNSWELHKEKSSNAGKNGRVPDFVF 300
           YL+YTP+Y +LHH EKDSNFCLFMPLF A+G TL+  SW+L KE   + GKN RVPDFVF
Sbjct: 245 YLIYTPSYLSLHHREKDSNFCLFMPLFDALGGTLNPKSWQLQKE--VDLGKNHRVPDFVF 304

Query: 301 LAHVVDVTSSMHAPFVSRFFASRPFVIKFSLFPLWPLAFITMLLMWGRSKIFLFTYYHLR 360
           L HVVDV SSMH PF  R  +S PF     L PLWP+AF  MLL W  SK F  ++Y LR
Sbjct: 305 LVHVVDVVSSMHVPFAFRACSSLPFATHLVLLPLWPIAFGFMLLQWFCSKTFTVSFYKLR 364

Query: 361 DWLHQTWVVPRFGFQYFLPFAREGINKHIEDAILRADKLGVKVISLSALNK--------- 420
            +LHQTW VPR+GFQYF+P A++GIN+ IE AILRADK+GVKV+SL+ALNK         
Sbjct: 365 GFLHQTWSVPRYGFQYFIPSAKKGINEMIELAILRADKMGVKVLSLAALNKNEALNGGGT 424

Query: 421 ------------------------------------------------------------ 480
                                                                       
Sbjct: 425 LFVRKHPDLRVRVVHGNTLTAAVILNEIPGDVAEVFLTGATSKLGRAIALYLCRKKIRVL 484

Query: 481 MLTLSTERFEKIQKEAPADCQNYLVQVTKYQRCPIASVVKLKQTWIVGKWITPREQSWAP 540
           MLTLSTERF  IQ+EAPA+ Q YLVQVTKYQ        +  +TWIVGKW++PREQ WAP
Sbjct: 485 MLTLSTERFMNIQREAPAEFQQYLVQVTKYQ------AAQNCKTWIVGKWLSPREQRWAP 544

Query: 541 SGTHFHQFVVPPILAFRRDCTYGELAAMRLPDDVQGLGNCEYTMSRGVVHACHAGGVVHH 559
           +GTHFHQFVVPPI+ FRRDCTYG+LAAMRLP+DV+GLG CEYTM RGVVHACHAGGVVH 
Sbjct: 545 AGTHFHQFVVPPIIGFRRDCTYGKLAAMRLPEDVEGLGTCEYTMGRGVVHACHAGGVVHF 604

BLAST of HG10008214 vs. ExPASy Swiss-Prot
Match: Q6ETL8 (Very-long-chain aldehyde decarbonylase GL1-2 OS=Oryza sativa subsp. japonica OX=39947 GN=GL1-2 PE=2 SV=1)

HSP 1 Score: 638.6 bits (1646), Expect = 6.4e-182
Identity = 337/634 (53.15%), Postives = 413/634 (65.14%), Query Frame = 0

Query: 4   PLSSWPWENLGMFKYLLYGPLVGKGLYSLYEEGNI-INNWCLHILLISLLRVGIHVAWSS 63
           PLSSWPW +LG +KY+LYG +V K      ++G   + +W LH+LL+   R   +  W S
Sbjct: 5   PLSSWPWASLGSYKYVLYGAVVWKVAEEWRQQGAAPVGSWWLHLLLLFAARGLTYQFWFS 64

Query: 64  YSNMLFLTRNRRILQQGVDFKQIDMEWEWDNFLLLQALMASMMI---------YLFPSLG 123
           Y NMLF TR RR++   VDF+Q+D EW+WDNFLLLQ L+ + ++          L PSL 
Sbjct: 65  YGNMLFFTRRRRVVPDSVDFRQVDAEWDWDNFLLLQTLIGATLVGSPAVARQQLLLPSLK 124

Query: 124 NLPLWNTKGLIAVQILHVGIAEPLFYIFHRFFHTNHYLFTYYHSLHHSSSVPQSFTAGNG 183
               W+ +G     +LHV +AEPLFY  HR  H    LF+ YH+ HH +SV    TAG G
Sbjct: 125 Q--AWDPRGWAIALLLHVLVAEPLFYWAHRALH-RAPLFSRYHAAHHHASVTTPLTAGFG 184

Query: 184 TVLEHLALSMVIGAPILGTSLLGYGSTAMIVCYVLVFDFLRCLGLSNVEIVPHRLFEAIP 243
           T LE L L++VIG P+ G  L+G GS  ++  +VL+FDFLR +G SNVE++  R+F+A+P
Sbjct: 185 TPLESLLLTVVIGVPLAGAFLMGVGSVGLVYGHVLLFDFLRSMGYSNVEVISPRVFQAVP 244

Query: 244 IFRYLLYTPTYHTLHHTEKDSNFCLFMPLFYAIGNTLHKNSWELHKEKSSNAGKNGRVPD 303
           + RYL+YTPTY +LHH EKDSNFCLFMP+F  +G TL+  SWEL KE     GKN + PD
Sbjct: 245 LLRYLIYTPTYLSLHHREKDSNFCLFMPIFDLLGGTLNHKSWELQKE--VYLGKNDQAPD 304

Query: 304 FVFLAHVVDVTSSMHAPFVSRFFASRPFVIKFSLFPLWPLAFITMLLMWGRSKIFLFTYY 363
           FVFLAHVVD+ +SMH PFV R  +S PF   F L P WP+AF  MLLMW  SK FL + Y
Sbjct: 305 FVFLAHVVDIMASMHVPFVLRSCSSTPFANHFVLLPFWPVAFGFMLLMWCCSKTFLVSSY 364

Query: 364 HLRDWLHQTWVVPRFGFQYFLPFAREGINKHIEDAILRADKLGVKVISLSALNK------ 423
            LR  LHQ W VPR+GFQYF+P A++GIN+ IE AILRAD++GVKV+SL+ALNK      
Sbjct: 365 RLRGNLHQMWTVPRYGFQYFIPAAKKGINEQIELAILRADRMGVKVLSLAALNKNEALNG 424

Query: 424 ------------------------------------------------------------ 483
                                                                       
Sbjct: 425 GGTLFVNKHPELRVRVVHGNTLTAAVILNEIPSNVKDVFLTGATSKLGRAIALYLCRKKI 484

Query: 484 ---MLTLSTERFEKIQKEAPADCQNYLVQVTKYQRCPIASVVKLKQTWIVGKWITPREQS 543
              MLTLS+ERF KIQ+EAPA+ Q YLVQVTKYQ  P  +     +TW+VGKW++PREQ 
Sbjct: 485 RVLMLTLSSERFLKIQREAPAEFQQYLVQVTKYQ--PAQNC----KTWLVGKWLSPREQR 544

Query: 544 WAPSGTHFHQFVVPPILAFRRDCTYGELAAMRLPDDVQGLGNCEYTMSRGVVHACHAGGV 559
           WAP+GTHFHQFVVPPI+ FRRDCTYG+LAAMRLP DVQGLG CEYTM RGVVHACHAGGV
Sbjct: 545 WAPAGTHFHQFVVPPIIGFRRDCTYGKLAAMRLPKDVQGLGYCEYTMERGVVHACHAGGV 604

BLAST of HG10008214 vs. ExPASy TrEMBL
Match: A0A0A0LZ62 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G294020 PE=3 SV=1)

HSP 1 Score: 1009.2 bits (2608), Expect = 6.7e-291
Identity = 499/631 (79.08%), Postives = 527/631 (83.52%), Query Frame = 0

Query: 1   MVAPLSSWPWENLGMFKYLLYGPLVGKGLYSLYEEGNIINNWCLHILLISLLRVGIHVAW 60
           MVAPL+SWPWENLGMFKYLLYGPL+  GLY+LYEEGNII+NWCLHILLISLLRVGIHV W
Sbjct: 1   MVAPLASWPWENLGMFKYLLYGPLLANGLYTLYEEGNIIHNWCLHILLISLLRVGIHVVW 60

Query: 61  SSYSNMLFLTRNRRILQQGVDFKQIDMEWEWDNFLLLQALMASMMIYLFPSLGNLPLWNT 120
           SSYSNMLFLTRNRRILQQGVDFKQIDMEWEWDNFLLLQALM SMM+YLFPSLGNLPLWN 
Sbjct: 61  SSYSNMLFLTRNRRILQQGVDFKQIDMEWEWDNFLLLQALMTSMMVYLFPSLGNLPLWNP 120

Query: 121 KGLIAVQILHVGIAEPLFYIFHRFFHTNHYLFTYYHSLHHSSSVPQSFTAGNGTVLEHLA 180
           KGLIAV ILH+ IAEPLFY FHR FH+NHYLFT+YHSLHHSSSVPQSFTAGNGTVLEHLA
Sbjct: 121 KGLIAVLILHIVIAEPLFYFFHRLFHSNHYLFTHYHSLHHSSSVPQSFTAGNGTVLEHLA 180

Query: 181 LSMVIGAPILGTSLLGYGSTAMIVCYVLVFDFLRCLGLSNVEIVPHRLFEAIPIFRYLLY 240
            S+VIGAPI+GTSLLGYGSTA   CYVLVFDFLRCLGLSNVEIV HRLF+AIP+ RYLLY
Sbjct: 181 WSIVIGAPIVGTSLLGYGSTATFACYVLVFDFLRCLGLSNVEIVSHRLFDAIPVLRYLLY 240

Query: 241 TPTYHTLHHTEKDSNFCLFMPLFYAIGNTLHKNSWELHKEKSSNAGKNGRVPDFVFLAHV 300
           TPTYHTLHHTEK++NFCLFMPLF AIGNTLHK SW+LHK+ S NAGKNGRVPDFVFLAHV
Sbjct: 241 TPTYHTLHHTEKETNFCLFMPLFDAIGNTLHKCSWKLHKQNSLNAGKNGRVPDFVFLAHV 300

Query: 301 VDVTSSMHAPFVSRFFASRPFVIKFSLFPLWPLAFITMLLMWGRSKIFLFTYYHLRDWLH 360
           VDVTSSMHAPFVSRFFASRPFV K SLFP WP AFI ML+MWGRSKIFL++YY+LR+WLH
Sbjct: 301 VDVTSSMHAPFVSRFFASRPFVTKLSLFPSWPAAFIVMLIMWGRSKIFLYSYYNLRNWLH 360

Query: 361 QTWVVPRFGFQYFLPFAREGINKHIEDAILRADKLGVKVISLSALNK------------- 420
           QTWVVPRFGFQYFLPFAREGINKHIEDAILRADKLGVKVISL+ALNK             
Sbjct: 361 QTWVVPRFGFQYFLPFAREGINKHIEDAILRADKLGVKVISLAALNKNEALNGGGTLFVE 420

Query: 421 --------------------------------------------------------MLTL 480
                                                                   MLTL
Sbjct: 421 KHPNLRVRVVHGNTLTAAVILNEIPKDVKEVFLTGATSKLGRAIALYLCRRKVRVLMLTL 480

Query: 481 STERFEKIQKEAPADCQNYLVQVTKYQRCPIASVVKLKQTWIVGKWITPREQSWAPSGTH 540
           STERFEKIQKEAP DCQNYLVQVTKYQ        +  +TWIVGKWITPREQSWAPSGTH
Sbjct: 481 STERFEKIQKEAPVDCQNYLVQVTKYQ------AARNCKTWIVGKWITPREQSWAPSGTH 540

Query: 541 FHQFVVPPILAFRRDCTYGELAAMRLPDDVQGLGNCEYTMSRGVVHACHAGGVVHHLEGW 563
           FHQFVVPPILAFRRDCTYG+LAAMRLP+DVQGLGNCEYTMSRGVVHACHAGGVVHHLEGW
Sbjct: 541 FHQFVVPPILAFRRDCTYGDLAAMRLPEDVQGLGNCEYTMSRGVVHACHAGGVVHHLEGW 600

BLAST of HG10008214 vs. ExPASy TrEMBL
Match: A0A6J1E508 (protein ECERIFERUM 3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430006 PE=3 SV=1)

HSP 1 Score: 989.9 bits (2558), Expect = 4.2e-285
Identity = 488/631 (77.34%), Postives = 522/631 (82.73%), Query Frame = 0

Query: 1   MVAPLSSWPWENLGMFKYLLYGPLVGKGLYSLYEEGNIINNWCLHILLISLLRVGIHVAW 60
           +VAPLSSWPWENLG  KYLLYGPL+  G YSLY++GNI+ NWCLHILL+S+LR+GIHVAW
Sbjct: 3   VVAPLSSWPWENLGSLKYLLYGPLLANGFYSLYQDGNILQNWCLHILLLSILRMGIHVAW 62

Query: 61  SSYSNMLFLTRNRRILQQGVDFKQIDMEWEWDNFLLLQALMASMMIYLFPSLGNLPLWNT 120
           SSYSNMLFLTRNRRILQQGVDFKQIDMEWEWDNFL+L +LMASMM+YLFP LGNLPLWNT
Sbjct: 63  SSYSNMLFLTRNRRILQQGVDFKQIDMEWEWDNFLILHSLMASMMVYLFPWLGNLPLWNT 122

Query: 121 KGLIAVQILHVGIAEPLFYIFHRFFHTNHYLFTYYHSLHHSSSVPQSFTAGNGTVLEHLA 180
           KGLIA+ ILHVGIAEPLFY+FHRFFHT HYLFT+YHSLHHSS VPQSFTAGN T LEHLA
Sbjct: 123 KGLIAILILHVGIAEPLFYVFHRFFHT-HYLFTHYHSLHHSSPVPQSFTAGNATFLEHLA 182

Query: 181 LSMVIGAPILGTSLLGYGSTAMIVCYVLVFDFLRCLGLSNVEIVPHRLFEAIPIFRYLLY 240
            S+VIGAPILGTSLLGYGST MI CYVLVFDFLRCLGLSNVEIVPHRLFEA+PI RYLLY
Sbjct: 183 WSLVIGAPILGTSLLGYGSTTMIFCYVLVFDFLRCLGLSNVEIVPHRLFEAVPILRYLLY 242

Query: 241 TPTYHTLHHTEKDSNFCLFMPLFYAIGNTLHKNSWELHKEKSSNAGKNGRVPDFVFLAHV 300
           TPTYHTLH TEK SNFCLFMPLF AIGNT+HKNSWELHKE SS AGKNG+VPDFVFLAHV
Sbjct: 243 TPTYHTLHRTEKGSNFCLFMPLFDAIGNTVHKNSWELHKEMSSTAGKNGKVPDFVFLAHV 302

Query: 301 VDVTSSMHAPFVSRFFASRPFVIKFSLFPLWPLAFITMLLMWGRSKIFLFTYYHLRDWLH 360
           VD+TSSMHAPFVSRFFASRPFV K SLFPLWP+AFI ML+MWGRSK FL+++Y+LRDWLH
Sbjct: 303 VDITSSMHAPFVSRFFASRPFVTKLSLFPLWPIAFIVMLVMWGRSKPFLYSFYNLRDWLH 362

Query: 361 QTWVVPRFGFQYFLPFAREGINKHIEDAILRADKLGVKVISLSALNK------------- 420
           QTW VPRFGFQYFLPFAREGIN HIE+AILRADKLGVKVISL+ALNK             
Sbjct: 363 QTWAVPRFGFQYFLPFAREGINNHIEEAILRADKLGVKVISLAALNKNEALNGGGTLFVE 422

Query: 421 --------------------------------------------------------MLTL 480
                                                                   MLTL
Sbjct: 423 KHPNLRVRVVHGNTLTAAVILNEIPKDVKEVFLTGATSKLGRAIALYLCRRKVRVLMLTL 482

Query: 481 STERFEKIQKEAPADCQNYLVQVTKYQRCPIASVVKLKQTWIVGKWITPREQSWAPSGTH 540
           +TERFEKIQKEAP +CQ+YLVQVTKYQ        +  +TWIVGKWITPREQSWAPSGTH
Sbjct: 483 ATERFEKIQKEAPTECQSYLVQVTKYQ------AARNCKTWIVGKWITPREQSWAPSGTH 542

Query: 541 FHQFVVPPILAFRRDCTYGELAAMRLPDDVQGLGNCEYTMSRGVVHACHAGGVVHHLEGW 563
           FHQFVVPPILAFRRDCTYG+LAAMRLPDDVQGLG CEYTMSRGVVHACHAGGVVHHLEGW
Sbjct: 543 FHQFVVPPILAFRRDCTYGDLAAMRLPDDVQGLGYCEYTMSRGVVHACHAGGVVHHLEGW 602

BLAST of HG10008214 vs. ExPASy TrEMBL
Match: A0A1S3B8V5 (protein ECERIFERUM 3 OS=Cucumis melo OX=3656 GN=LOC103487308 PE=3 SV=1)

HSP 1 Score: 981.5 bits (2536), Expect = 1.5e-282
Identity = 486/617 (78.77%), Postives = 517/617 (83.79%), Query Frame = 0

Query: 15  MFKYLLYGPLVGKGLYSLYEEGNIINNWCLHILLISLLRVGIHVAWSSYSNMLFLTRNRR 74
           MFKYLLYGPL+  GLY+LYEEGNII+NWCLHILLISLLRVGIHV WSSYSNMLFLTRNRR
Sbjct: 1   MFKYLLYGPLLANGLYTLYEEGNIIHNWCLHILLISLLRVGIHVVWSSYSNMLFLTRNRR 60

Query: 75  ILQQGVDFKQIDMEWEWDNFLLLQALMASMMIYLFPSLGNLPLWNTKGLIAVQILHVGIA 134
           ILQQGVDFKQIDMEWEWDNFLLL+ALM SMM+YLFPSLGNLPLWNTKGLIA+ ILH+ IA
Sbjct: 61  ILQQGVDFKQIDMEWEWDNFLLLEALMTSMMVYLFPSLGNLPLWNTKGLIALLILHIVIA 120

Query: 135 EPLFYIFHRFFHTNHYLFTYYHSLHHSSSVPQSFTAGNGTVLEHLALSMVIGAPILGTSL 194
           EPLFY FHR FH+NHYLFT+YHSLHHSSSVPQSFTAGNGTVLEHLA S+VIGAPI+GT L
Sbjct: 121 EPLFYFFHRLFHSNHYLFTHYHSLHHSSSVPQSFTAGNGTVLEHLAWSIVIGAPIVGTFL 180

Query: 195 LGYGSTAMIVCYVLVFDFLRCLGLSNVEIVPHRLFEAIPIFRYLLYTPTYHTLHHTEKDS 254
           LGYGSTA I CYVLVFDFLRCLGLSNVEIV HRLF+AIP+ RYLLYTPTYHTLHHTEK++
Sbjct: 181 LGYGSTATIACYVLVFDFLRCLGLSNVEIVSHRLFDAIPVLRYLLYTPTYHTLHHTEKET 240

Query: 255 NFCLFMPLFYAIGNTLHKNSWELHKEKSSNAGKNGRVPDFVFLAHVVDVTSSMHAPFVSR 314
           NFCLFMPLF AIGNTLH+NSW+LHK+ S NAGKNGRVPDFVFLAHVVDVTSSMHAPFVSR
Sbjct: 241 NFCLFMPLFDAIGNTLHENSWKLHKQNSLNAGKNGRVPDFVFLAHVVDVTSSMHAPFVSR 300

Query: 315 FFASRPFVIKFSLFPLWPLAFITMLLMWGRSKIFLFTYYHLRDWLHQTWVVPRFGFQYFL 374
           FFASRPFV K SLFP WP+AFI ML+MWGRSKIFL++YY+LR+WLHQTWVVPRFGFQYFL
Sbjct: 301 FFASRPFVTKLSLFPSWPVAFIVMLIMWGRSKIFLYSYYNLRNWLHQTWVVPRFGFQYFL 360

Query: 375 PFAREGINKHIEDAILRADKLGVKVISLSALNK--------------------------- 434
           PFAREGINKHIEDAILRADKLGVKVISL+ALNK                           
Sbjct: 361 PFAREGINKHIEDAILRADKLGVKVISLAALNKNEALNGGGTLFVEKHPNLRVRVVHGNT 420

Query: 435 ------------------------------------------MLTLSTERFEKIQKEAPA 494
                                                     MLTLSTERFEKIQKEAPA
Sbjct: 421 LTAAVILNEIPKDVKEVFLTGATSKLGRAIALYLCRRKVRVLMLTLSTERFEKIQKEAPA 480

Query: 495 DCQNYLVQVTKYQRCPIASVVKLKQTWIVGKWITPREQSWAPSGTHFHQFVVPPILAFRR 554
           DCQNYLVQVTKYQ        +  +TWIVGKWITPREQSWAPSGTHFHQFVVPPILAFRR
Sbjct: 481 DCQNYLVQVTKYQ------AARNCKTWIVGKWITPREQSWAPSGTHFHQFVVPPILAFRR 540

Query: 555 DCTYGELAAMRLPDDVQGLGNCEYTMSRGVVHACHAGGVVHHLEGWSHHEVGALDVDRID 563
           DCTYG+LAAMRLP+DVQGLGNCEYTMSRGVVHACHAGGVVHHLEGW+HHEVGALDVDRID
Sbjct: 541 DCTYGDLAAMRLPEDVQGLGNCEYTMSRGVVHACHAGGVVHHLEGWTHHEVGALDVDRID 600

BLAST of HG10008214 vs. ExPASy TrEMBL
Match: A0A6J1JJW6 (protein ECERIFERUM 3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485764 PE=3 SV=1)

HSP 1 Score: 978.4 bits (2528), Expect = 1.3e-281
Identity = 482/631 (76.39%), Postives = 520/631 (82.41%), Query Frame = 0

Query: 1   MVAPLSSWPWENLGMFKYLLYGPLVGKGLYSLYEEGNIINNWCLHILLISLLRVGIHVAW 60
           +VAPLSSWPWENLG  K LLYGPL+  G YSLY++GNI+ NWCLHILL+SLLR+GIHVAW
Sbjct: 3   VVAPLSSWPWENLGSLKCLLYGPLLANGFYSLYQDGNILQNWCLHILLLSLLRMGIHVAW 62

Query: 61  SSYSNMLFLTRNRRILQQGVDFKQIDMEWEWDNFLLLQALMASMMIYLFPSLGNLPLWNT 120
           SSYSNMLFLTRNRRI+QQGVDFKQIDMEWEWDNFL+LQ+LMASMM+YLFP LGNLPLW T
Sbjct: 63  SSYSNMLFLTRNRRIIQQGVDFKQIDMEWEWDNFLILQSLMASMMVYLFPWLGNLPLWKT 122

Query: 121 KGLIAVQILHVGIAEPLFYIFHRFFHTNHYLFTYYHSLHHSSSVPQSFTAGNGTVLEHLA 180
           KGLI + ILHVGIAEPLFY+FHRFFHT HYLFT+YHSLHHSS VPQSFTAGN T LEHLA
Sbjct: 123 KGLITILILHVGIAEPLFYVFHRFFHT-HYLFTHYHSLHHSSPVPQSFTAGNATFLEHLA 182

Query: 181 LSMVIGAPILGTSLLGYGSTAMIVCYVLVFDFLRCLGLSNVEIVPHRLFEAIPIFRYLLY 240
            S+VIGAPILGTSLLGYGST MI CYVLVFDFLRCLGLSNVEIVPHRLFEA+PI RYLLY
Sbjct: 183 WSLVIGAPILGTSLLGYGSTIMIFCYVLVFDFLRCLGLSNVEIVPHRLFEAVPILRYLLY 242

Query: 241 TPTYHTLHHTEKDSNFCLFMPLFYAIGNTLHKNSWELHKEKSSNAGKNGRVPDFVFLAHV 300
           TPTYHTLH TEK++NFCLFMPLF AIGNT+HKNSWELHKE SS AGKNG+VPDFVFLAHV
Sbjct: 243 TPTYHTLHRTEKETNFCLFMPLFDAIGNTVHKNSWELHKEMSSKAGKNGKVPDFVFLAHV 302

Query: 301 VDVTSSMHAPFVSRFFASRPFVIKFSLFPLWPLAFITMLLMWGRSKIFLFTYYHLRDWLH 360
           VD+TSSMHAPFVSRFFASRPFV K SL PLWP+ FI ML+MWGRSK FL+++Y+LRDWLH
Sbjct: 303 VDITSSMHAPFVSRFFASRPFVTKLSLIPLWPITFIVMLVMWGRSKPFLYSFYNLRDWLH 362

Query: 361 QTWVVPRFGFQYFLPFAREGINKHIEDAILRADKLGVKVISLSALNK------------- 420
           QTWVVPRFGFQYFLPFAREGIN HIE+AILRADKLGVKVISL+ALNK             
Sbjct: 363 QTWVVPRFGFQYFLPFAREGINNHIEEAILRADKLGVKVISLAALNKNEALNGGGTLFVE 422

Query: 421 --------------------------------------------------------MLTL 480
                                                                   MLTL
Sbjct: 423 KHPNLRVRVVHGNTLTAAVILNEIPKDAKEVFLTGATSKLGRAIALYLCRRKVRVLMLTL 482

Query: 481 STERFEKIQKEAPADCQNYLVQVTKYQRCPIASVVKLKQTWIVGKWITPREQSWAPSGTH 540
           +TERFEKIQKEAP +CQ+YLVQVTKYQ        +  +TWIVGKWITPREQSWAPSGTH
Sbjct: 483 ATERFEKIQKEAPTECQSYLVQVTKYQ------AARNCKTWIVGKWITPREQSWAPSGTH 542

Query: 541 FHQFVVPPILAFRRDCTYGELAAMRLPDDVQGLGNCEYTMSRGVVHACHAGGVVHHLEGW 563
           FHQFVVPPILAFRRDCTYG+LAAM+LP+DVQGLG CEYTMSRGVVHACHAGGVVHHLEGW
Sbjct: 543 FHQFVVPPILAFRRDCTYGDLAAMQLPNDVQGLGYCEYTMSRGVVHACHAGGVVHHLEGW 602

BLAST of HG10008214 vs. ExPASy TrEMBL
Match: A0A6J1BYY3 (protein ECERIFERUM 3 OS=Momordica charantia OX=3673 GN=LOC111007024 PE=3 SV=1)

HSP 1 Score: 976.5 bits (2523), Expect = 4.8e-281
Identity = 486/630 (77.14%), Postives = 517/630 (82.06%), Query Frame = 0

Query: 1   MVAPLSSWPWENLGMFKYLLYGPLVGKGLYSLYEEGNIINNWCLHILLISLLRVGIHVAW 60
           MVAPLSSWPWENLG+FKY+LYGPLV  GLYSLYEEGNI+NNWCLHI+LISLLRVGIH  W
Sbjct: 1   MVAPLSSWPWENLGIFKYVLYGPLVANGLYSLYEEGNIVNNWCLHIVLISLLRVGIHTCW 60

Query: 61  SSYSNMLFLTRNRRILQQGVDFKQIDMEWEWDNFLLLQALMASMMIYLFPSLGNLPLWNT 120
           SSYS MLFLTRNR+ILQQGVDFKQIDME++WDNFLLLQAL++SM+IYLFP LGNLPLWNT
Sbjct: 61  SSYSTMLFLTRNRQILQQGVDFKQIDMEFDWDNFLLLQALISSMVIYLFPWLGNLPLWNT 120

Query: 121 KGLIAVQILHVGIAEPLFYIFHRFFHTNHYLFTYYHSLHHSSSVPQSFTAGNGTVLEHLA 180
           KG IAV ILHVG+AEPLFY+ HRFFHT+ Y FT+YHSLHHSS VPQ FTAGNGTVLE LA
Sbjct: 121 KGFIAVLILHVGVAEPLFYVLHRFFHTD-YFFTHYHSLHHSSPVPQPFTAGNGTVLEQLA 180

Query: 181 LSMVIGAPILGTSLLGYGSTAMIVCYVLVFDFLRCLGLSNVEIVPHRLFEAIPIFRYLLY 240
           LS+VIGAPILGTSLLGYGSTAMI CYVL+FDFLRCLG  NVEI PHRLFEAIPIFRYLLY
Sbjct: 181 LSLVIGAPILGTSLLGYGSTAMIFCYVLIFDFLRCLGHCNVEIFPHRLFEAIPIFRYLLY 240

Query: 241 TPTYHTLHHTEKDSNFCLFMPLFYAIGNTLHKNSWELHKEKSSNAGKNGRVPDFVFLAHV 300
           TPTYHTLHHTEK +NFCLFMPLF AIGNTLHKNSW+L KE S N+GK G+VPDFVFLAHV
Sbjct: 241 TPTYHTLHHTEKGTNFCLFMPLFDAIGNTLHKNSWDLQKEISLNSGKRGKVPDFVFLAHV 300

Query: 301 VDVTSSMHAPFVSRFFASRPFVIKFSLFPLWPLAFITMLLMWGRSKIFLFTYYHLRDWLH 360
           VDVTSSMHA FVSRFFASRPFV K SL P WP+AFI MLLMWGRSK FL+++Y+LR WLH
Sbjct: 301 VDVTSSMHASFVSRFFASRPFVTKLSLLPQWPIAFIVMLLMWGRSKTFLYSFYNLRGWLH 360

Query: 361 QTWVVPRFGFQYFLPFAREGINKHIEDAILRADKLGVKVISLSALNK------------- 420
           QTWVVPRFGFQYFLPFAREGINKHIEDAILRADKLGVKVISL+ALNK             
Sbjct: 361 QTWVVPRFGFQYFLPFAREGINKHIEDAILRADKLGVKVISLAALNKNEALNGGGTLFVE 420

Query: 421 --------------------------------------------------------MLTL 480
                                                                   MLTL
Sbjct: 421 KHPNLRVRVVHGNTLTAAVILNEIPKDVKEVFLTGATSKLGRAIALYLCRRKVRVLMLTL 480

Query: 481 STERFEKIQKEAPADCQNYLVQVTKYQRCPIASVVKLKQTWIVGKWITPREQSWAPSGTH 540
           S ERFEKIQKEAPADCQNYLVQVTKYQ        +  +TWIVGKWITPREQSWAPSGTH
Sbjct: 481 SAERFEKIQKEAPADCQNYLVQVTKYQ------AARNCKTWIVGKWITPREQSWAPSGTH 540

Query: 541 FHQFVVPPILAFRRDCTYGELAAMRLPDDVQGLGNCEYTMSRGVVHACHAGGVVHHLEGW 562
           FHQFVVPPILAFRRDCTYG+LAAMRLPDDVQGLGNCEYTM RGVVHACHAGGVVHHLEGW
Sbjct: 541 FHQFVVPPILAFRRDCTYGDLAAMRLPDDVQGLGNCEYTMDRGVVHACHAGGVVHHLEGW 600

BLAST of HG10008214 vs. TAIR 10
Match: AT5G57800.1 (Fatty acid hydroxylase superfamily )

HSP 1 Score: 710.7 bits (1833), Expect = 9.4e-205
Identity = 362/638 (56.74%), Postives = 435/638 (68.18%), Query Frame = 0

Query: 1   MVAPLSSWPWENLGMFKYLLYGPLVGKGLYS-LYEEGNIINNWCLHILLISLLRVGIHVA 60
           MVA LS+WPWEN G  KYLLY PL  + +YS +YEE      WC+HIL+I  L+  +H  
Sbjct: 1   MVAFLSAWPWENFGNLKYLLYAPLAAQVVYSWVYEEDISKVLWCIHILIICGLKALVHEL 60

Query: 61  WSSYSNMLFLTRNRRILQQGVDFKQIDMEWEWDNFLLLQALMASMMIYLFPSL----GNL 120
           WS ++NMLF+TR  RI  +G+DFKQID EW WDN+++LQA++ S++ Y+ P L     +L
Sbjct: 61  WSVFNNMLFVTRTLRINPKGIDFKQIDHEWHWDNYIILQAIIVSLICYMSPPLMMMINSL 120

Query: 121 PLWNTKGLIAVQILHVGIAEPLFYIFHRFFHTNHYLFTYYHSLHHSSSVPQSFTAGNGTV 180
           PLWNTKGLIA+ +LHV  +EPL+Y  HR FH N+Y FT+YHS HHSS VP   TAGN T+
Sbjct: 121 PLWNTKGLIALIVLHVTFSEPLYYFLHRSFHRNNYFFTHYHSFHHSSPVPHPMTAGNATL 180

Query: 181 LEHLALSMVIGAPILGTSLLGYGSTAMIVCYVLVFDFLRCLGLSNVEIVPHRLFEAIPIF 240
           LE++ L +V G P++G  L G GS + I  Y ++FDF+RCLG  NVEI  H+LFE +P+ 
Sbjct: 181 LENIILCVVAGVPLIGCCLFGVGSLSAIYGYAVMFDFMRCLGHCNVEIFSHKLFEILPVL 240

Query: 241 RYLLYTPTYHTLHHTEKDSNFCLFMPLFYAIGNTLHKNSWELHKEKSSNAGKNGRVPDFV 300
           RYL+YTPTYH+LHH E  +NFCLFMPLF  +G+T + NSWEL K+   +AG+  RVP+FV
Sbjct: 241 RYLIYTPTYHSLHHQEMGTNFCLFMPLFDVLGDTQNPNSWELQKKIRLSAGERKRVPEFV 300

Query: 301 FLAHVVDVTSSMHAPFVSRFFASRPFVIKFSLFPLWPLAFITMLLMWGRSKIFLFTYYHL 360
           FLAH VDV S+MHAPFV R FAS P+  +  L P+WP  F  ML MW  SK FLF++Y L
Sbjct: 301 FLAHGVDVMSAMHAPFVFRSFASMPYTTRIFLLPMWPFTFCVMLGMWAWSKTFLFSFYTL 360

Query: 361 RDWLHQTWVVPRFGFQYFLPFAREGINKHIEDAILRADKLGVKVISLSALNK-------- 420
           R+ L QTW VPRFGFQYFLPFA +GIN  IE AILRADK+GVKVISL+ALNK        
Sbjct: 361 RNNLCQTWGVPRFGFQYFLPFATKGINDQIEAAILRADKIGVKVISLAALNKNEALNGGG 420

Query: 421 ------------------------------------------------------------ 480
                                                                       
Sbjct: 421 TLFVNKHPDLRVRVVHGNTLTAAVILYEIPKDVNEVFLTGATSKLGRAIALYLCRRGVRV 480

Query: 481 -MLTLSTERFEKIQKEAPADCQNYLVQVTKY---QRCPIASVVKLKQTWIVGKWITPREQ 540
            MLTLS ERF+KIQKEAP + QN LVQVTKY   Q C         +TWIVGKW+TPREQ
Sbjct: 481 LMLTLSMERFQKIQKEAPVEFQNNLVQVTKYNAAQHC---------KTWIVGKWLTPREQ 540

Query: 541 SWAPSGTHFHQFVVPPILAFRRDCTYGELAAMRLPDDVQGLGNCEYTMSRGVVHACHAGG 562
           SWAP+GTHFHQFVVPPIL FRR+CTYG+LAAM+LP DV+GLG CEYTM RGVVHACHAGG
Sbjct: 541 SWAPAGTHFHQFVVPPILKFRRNCTYGDLAAMKLPKDVEGLGTCEYTMERGVVHACHAGG 600

BLAST of HG10008214 vs. TAIR 10
Match: AT1G02205.2 (Fatty acid hydroxylase superfamily )

HSP 1 Score: 301.6 bits (771), Expect = 1.3e-81
Identity = 190/624 (30.45%), Postives = 295/624 (47.28%), Query Frame = 0

Query: 5   LSSWPWENLGMFKYLLYGPLVGKGLYSLYEEGNIINNWCLHILLISLL-RVGIHVAWSSY 64
           L+ WPW  LG FKY++  P      Y    +     +    ++   LL R+  +  W S 
Sbjct: 8   LTDWPWTPLGSFKYIVIAPWAVHSTYRFVTDDPEKRDLGYFLVFPFLLFRILHNQVWISL 67

Query: 65  SNMLFLTRNRRILQQGVDFKQIDMEWEWDNFLLLQALMASMMIYLFPSLGNLPLWNTKGL 124
           S     +  RRI+ +G+DF Q+D E  WD+ +L   ++  + I L P    LP W T G+
Sbjct: 68  SRYYTSSGKRRIVDKGIDFNQVDRETNWDDQILFNGVLFYIGINLLPEAKQLPWWRTDGV 127

Query: 125 IAVQILHVGIAEPLFYIFHRFFHTNHYLFTYYHSLHHSSSVPQSFTAGNGTVLEHLALSM 184
           +   ++H G  E L+Y  H+  H +H+L++ YHS HHSS V +  T+      EH+A  +
Sbjct: 128 LMAALIHTGPVEFLYYWLHKALH-HHFLYSRYHSHHHSSIVTEPITSVIHPFAEHIAYFI 187

Query: 185 VIGAPILGTSLLGYGSTAMIVCYVLVFDFLRCLGLSNVEIVPHRLFEAIPIFRYLLYTPT 244
           +   P+L T L    S      Y++  DF+  +G  N E++P RLF   P  ++L YTP+
Sbjct: 188 LFAIPLLTTLLTKTASIISFAGYIIYIDFMNNMGHCNFELIPKRLFHLFPPLKFLCYTPS 247

Query: 245 YHTLHHTEKDSNFCLFMPLFYAIGNTLHKNSWELHKEKSSNAGKNGRVPDFVFLAHVVDV 304
           YH+LHHT+  +N+ LFMPL+  I  T+ +++  L+ EK+   G +  + D V L H+   
Sbjct: 248 YHSLHHTQFRTNYSLFMPLYDYIYGTMDESTDTLY-EKTLERGDD--IVDVVHLTHLTTP 307

Query: 305 TSSMHAPFVSRFFASRPFVIKFSLFPLWPLAFITMLLMWGRSKIFLFTYYHLRDWLHQTW 364
            S  H       FAS PF  ++ +  LWP   ++M+     +++F+           Q+W
Sbjct: 308 ESIYHLRIGLASFASYPFAYRWFMRLLWPFTSLSMIFTLFYARLFVAERNSFNKLNLQSW 367

Query: 365 VVPRFGFQYFLPFAREGINKHIEDAILRADKLGVKVISLSALN----------------- 424
           V+PR+  QY L + +E IN  IE AIL ADK GVKV+SL  +N                 
Sbjct: 368 VIPRYNLQYLLKWRKEAINNMIEKAILEADKKGVKVLSLGLMNQGEELNRNGEVYIHNHP 427

Query: 425 --------------------------------------------------KMLTLSTERF 484
                                                             ++ TL  + +
Sbjct: 428 DMKVRLVDGSRLAAAVVINSVPKATTSVVMTGNLTKVAYTIASALCQRGVQVSTLRLDEY 487

Query: 485 EKIQKEAPADCQNYLVQVTKYQRCPIASVVKLKQTWIVGKWITPREQSWAPSGTHFHQFV 544
           EKI+   P +C+++LV +T       +  +   + W+VG+  T  EQ  A  GT F  F 
Sbjct: 488 EKIRSCVPQECRDHLVYLT-------SEALSSNKVWLVGEGTTREEQEKATKGTLFIPFS 547

Query: 545 VPPILAFRRDCTYGELAAMRLPDDVQGLGNCEYTMSRGVVHACHAGGVVHHLEGWSHHEV 560
             P+   RRDC Y    A+ +P  +  + +CE  + R  + A    G++H LEGW  HE 
Sbjct: 548 QFPLKQLRRDCIYHTTPALIVPKSLVNVHSCENWLPRKAMSATRVAGILHALEGWEMHEC 607

BLAST of HG10008214 vs. TAIR 10
Match: AT1G02205.3 (Fatty acid hydroxylase superfamily )

HSP 1 Score: 297.7 bits (761), Expect = 1.9e-80
Identity = 190/624 (30.45%), Postives = 295/624 (47.28%), Query Frame = 0

Query: 5   LSSWPWENLGMFKYLLYGPLVGKGLYSLYEEGNIINNWCLHILLISLL-RVGIHVAWSSY 64
           L+ WPW  LG FKY++  P      Y    +     +    ++   LL R+  +  W S 
Sbjct: 8   LTDWPWTPLGSFKYIVIAPWAVHSTYRFVTDDPEKRDLGYFLVFPFLLFRILHNQVWISL 67

Query: 65  SNMLFLTRNRRILQQGVDFKQIDMEWEWDNFLLLQALMASMMIYLFPSLGNLPLWNTKGL 124
           S     +  RRI+ +G+DF Q+D E  WD+ +L   ++  + I L P    LP W T G+
Sbjct: 68  SRYYTSSGKRRIVDKGIDFNQVDRETNWDDQILFNGVLFYIGINLLPEAKQLPWWRTDGV 127

Query: 125 IAVQILHVGIAEPLFYIFHRFFHTNHYLFTYYHSLHHSSSVPQSFTAGNGTVLEHLALSM 184
           +   ++H G  E L+Y  H+  H +H+L++ YHS HHSS V +  T+      EH+A  +
Sbjct: 128 LMAALIHTGPVEFLYYWLHKALH-HHFLYSRYHSHHHSSIVTEPITSVIHPFAEHIAYFI 187

Query: 185 VIGAPILGTSLLGYGSTAMIVCYVLVFDFLRCLGLSNVEIVPHRLFEAIPIFRYLLYTPT 244
           +   P+L T L    S      Y++  DF+  +G  N E++P RLF   P  ++L YTP+
Sbjct: 188 LFAIPLLTTLLTKTASIISFAGYIIYIDFMNNMGHCNFELIPKRLFHLFPPLKFLCYTPS 247

Query: 245 YHTLHHTEKDSNFCLFMPLFYAIGNTLHKNSWELHKEKSSNAGKNGRVPDFVFLAHVVDV 304
           YH+LHHT+  +N+ LFMPL+  I  T+ +++  L+ EK+   G +  + D V L H+   
Sbjct: 248 YHSLHHTQFRTNYSLFMPLYDYIYGTMDESTDTLY-EKTLERGDD--IVDVVHLTHLTTP 307

Query: 305 TSSMHAPFVSRFFASRPFVIKFSLFPLWPLAFITMLLMWGRSKIFLFTYYHLRDWLHQTW 364
            S  H       FAS PF  ++ +  LWP   ++M+     +++F+           Q+W
Sbjct: 308 ESIYHLRIGLASFASYPFAYRWFMRLLWPFTSLSMIFTLFYARLFVAERNSFNKLNLQSW 367

Query: 365 VVPRFGFQYFLPFAREGINKHIEDAILRADKLGVKVISLSALN----------------- 424
           V+PR+  QY L + +E IN  IE AIL ADK GVKV+SL  +N                 
Sbjct: 368 VIPRYNLQYLLKWRKEAINNMIEKAILEADKKGVKVLSLGLMNQGEELNRNGEVYIHNHP 427

Query: 425 --------------------------------------------------KMLTLSTERF 484
                                                             ++ TL  + +
Sbjct: 428 DMKVRLVDGSRLAAAVVINSVPKATTSVVMTGNLTKVAYTIASALCQRGVQVSTLRLDEY 487

Query: 485 EKIQKEAPADCQNYLVQVTKYQRCPIASVVKLKQTWIVGKWITPREQSWAPSGTHFHQFV 544
           EKI+   P +C+++LV +T       ++     + W+VG+  T  EQ  A  GT F  F 
Sbjct: 488 EKIRSCVPQECRDHLVYLT--SEALSSNKGFWVKVWLVGEGTTREEQEKATKGTLFIPFS 547

Query: 545 VPPILAFRRDCTYGELAAMRLPDDVQGLGNCEYTMSRGVVHACHAGGVVHHLEGWSHHEV 560
             P+   RRDC Y    A+ +P  +  + +CE  + R  + A    G++H LEGW  HE 
Sbjct: 548 QFPLKQLRRDCIYHTTPALIVPKSLVNVHSCENWLPRKAMSATRVAGILHALEGWEMHEC 607

BLAST of HG10008214 vs. TAIR 10
Match: AT2G37700.1 (Fatty acid hydroxylase superfamily )

HSP 1 Score: 287.0 bits (733), Expect = 3.4e-77
Identity = 193/606 (31.85%), Postives = 293/606 (48.35%), Query Frame = 0

Query: 5   LSSWPWENLGMFKYLLYGPLVGKGLYSLYEEGNIINNWCLHILLISLLRVGIHVAWSSYS 64
           L+ WPW  LG FKYLL  PLV     S+Y    I ++  L I+ +++ R+     W S S
Sbjct: 8   LTDWPWTPLGSFKYLLLAPLV---FDSIYSYATIRDHEKLLIVAVTVWRIVHSQIWISLS 67

Query: 65  NMLFLTRNRRILQQGVDFKQIDMEWEWDNFLLLQALMASMMIYLFPSLGNLPLWNTKGLI 124
                   +RIL + ++F Q+D E  WD+ ++   L+  +          +P W T G+I
Sbjct: 68  RYQTAKGTKRILNKSIEFDQVDRERTWDDQIIFNTLIVYLTKVYVSGTSTIPFWRTDGVI 127

Query: 125 AVQILHVGIAEPLFYIFHRFFHTNHYLFTYYHSLHHSSSVPQSFTAGNGTVLEHLALSMV 184
            V +LH G  E ++Y FHR  H +H+L++ YHS HHSS V +  T+      EH+  +++
Sbjct: 128 LVALLHAGPVEFIYYWFHRALH-HHFLYSRYHSHHHSSIVTEPITSVVHPFAEHIGYTLI 187

Query: 185 IGAPILGTSLLGYGSTAMIVCYVLVFDFLRCLGLSNVEIVPHRLFEAIPIFRYLLYTPTY 244
           +G P++ T + G  S   I  Y+   DF+  +G  N E++P  LF  +P  ++L YTP++
Sbjct: 188 LGLPLITTFMCGTVSVVSIALYLTYIDFMNNMGHCNFELIPKFLFSLLPPLKFLCYTPSF 247

Query: 245 HTLHHTEKDSNFCLFMPLFYAIGNTLHKNSWELHKEKSSNAGKNGRVPDFVFLAHVVDVT 304
           H+LHHT+  +N+ LFMP++  I  T  + S  L++   ++  K    PD + L H+  + 
Sbjct: 248 HSLHHTQFRTNYSLFMPMYDYIYGTTDECSDSLYE---TSLEKEEEKPDAIHLTHLTSLD 307

Query: 305 SSMHAPFVSRFFASRPFVIKFSLFPLWPLAFITMLLMWGRS-KIFLFTYYHLRDWLHQTW 364
           S  H        +S P   +  LF + P A I   ++   S + F+      RD    + 
Sbjct: 308 SIYHLRLGFASLSSHPLSSRCYLFLMKPFALILSFILRSFSFQTFVVERNRFRDLTLHSH 367

Query: 365 VVPRFGFQYFLPFAREGINKHIEDAILRADKLGVKVISLSALNKMLTLS------TERFE 424
           ++P+F   Y     +E INK IE AIL ADK GVKV+SL  LN+   L+        R  
Sbjct: 368 LLPKFSSHYMSHQQKECINKMIEAAILEADKKGVKVMSLGLLNQGEELNGYGEMYVRRHP 427

Query: 425 KIQ---------------KEAPADCQNYLV--QVTKYQRCPIASVV-------------- 484
           K++                  P   +  L   Q+TK  R  + S+               
Sbjct: 428 KLKIRIVDGGSLAAEVVLHSIPVGTKEVLFRGQITKVARAIVFSLCQNAIKVMVLRKEEH 487

Query: 485 ---------KLKQT--WIVGKWITPREQSWAPSGTHFHQFVVPPILAFRRDCTYGELAAM 544
                    K K+   W+VG  ++ +EQ  A  GT F  F   P    R+DC Y    AM
Sbjct: 488 SMLAEFLDDKCKENLIWLVGDGLSTKEQKMAKDGTLFLPFSQFPPKTLRKDCFYHTTPAM 547

Query: 545 RLPDDVQGLGNCEYTMSRGVVHACHAGGVVHHLEGWSHHEVGALDVDRID--LVWEAALK 560
            +P   Q + +CE  + R V+ A   GG+VH LEGW  HE G  D   I+   VWEAAL+
Sbjct: 548 IIPHSAQNIDSCENWLGRRVMSAWRVGGIVHALEGWKEHECGLDDNSIINPPRVWEAALR 606

BLAST of HG10008214 vs. TAIR 10
Match: AT1G02190.1 (Fatty acid hydroxylase superfamily )

HSP 1 Score: 261.5 bits (667), Expect = 1.5e-69
Identity = 190/629 (30.21%), Postives = 290/629 (46.10%), Query Frame = 0

Query: 5   LSSWPWENLGMFKYLLYGPLVGKGLYS----LYEEGNIINNWCLHILLISLLRVGIHVAW 64
           L+ WPW  LG FKYLL  PLV   ++S    + EE ++     L I+++ L R+     W
Sbjct: 8   LTEWPWSPLGSFKYLLVAPLVMASMHSYVTAVDEEKDLSR---LMIVVLMLWRIVHSQIW 67

Query: 65  SSYSNMLFLTRNRRILQQGVDFKQIDMEWEWDNFLLLQALMASMMIYLFPSLGNLPLWNT 124
            S S         +I+ + ++F+Q+D E  WD+ ++   L+  +     P   +LP W  
Sbjct: 68  ISVSRQRTAKGTNKIVDKPIEFEQVDRERTWDDQVIFNTLLMYLANIKLPGASHLPPWRL 127

Query: 125 KGLIAVQILHVGIAEPLFYIFHRFFHTNHYLFTYYHSLHHSSSVPQSFTAGNGTVLEHLA 184
            G I + +LH G  E L+Y FHR  H +H+L++ YHS HHSS V +  T+      EH+A
Sbjct: 128 DGAILMALLHAGPVEFLYYWFHRALH-HHFLYSRYHSHHHSSIVTEPITSVVHPFAEHIA 187

Query: 185 LSMVIGAPILGTSLLGYGSTAMIVCYVLVFDFLRCLGLSNVEIVPHRLFEAIPIFRYLLY 244
            +++   P++  SL G  S   I+ Y+   DF+  +G  N E+ P RLF   P  ++L Y
Sbjct: 188 YTLLFAIPMVTASLCGILSIVSIMGYITYIDFMNNMGHCNFELFPKRLFHLFPPLKFLCY 247

Query: 245 TPTYHTLHHTEKDSNFCLFMPLF-YAIGNT------LHKNSWELHKEKSSNAGKNGRVPD 304
           TP++H+LHHT+  +N+ LFMP++ +  G T      L++ S E+ +E           PD
Sbjct: 248 TPSFHSLHHTQFRTNYSLFMPIYDFIYGTTDNLTDSLYERSLEIEEES----------PD 307

Query: 305 FVFLAHVVDVTSSMHAPFVSRFFASRPFVIK---FSLFPLWPLAFITMLLMWGR--SKIF 364
            + L H+    S           +S P   +   +    +WP   +    +      + F
Sbjct: 308 VIHLTHLTTHNSIYQMRLGFPSLSSCPLWSRPPWYLTCFMWPFTLLCSFALTSAIPLRTF 367

Query: 365 LFTYYHLRDWLHQTWVVPRFGFQYFLPFAREGINKHIEDAILRADKLGVKVISLSALNKM 424
           +F    LRD    + ++P+F F Y      E IN  IE+AIL AD+ GVKV+SL  +N  
Sbjct: 368 VFERNRLRDLTVHSHLLPKFSFHYKSQRHHESINTIIEEAILEADEKGVKVMSLGLMNNR 427

Query: 425 LTL-------------------------STERFEKIQKEAP-----------------AD 484
             L                         +T     I KEA                  A 
Sbjct: 428 EELNGSGEMYVQKYPKLKIRLVDGSSMAATVVINNIPKEATEIVFRGNLTKVASAVVFAL 487

Query: 485 CQN----YLVQVTKYQRCPIASVVK-----------LKQTWIVGKWITPREQSWAPSGTH 544
           CQ      +++  ++ +   + V K             + W+VG  I   EQ  A  GT 
Sbjct: 488 CQKGVKVVVLREEEHSKLIKSGVDKNLVLSTSNSYYSPKVWLVGDGIENEEQMKAKEGTL 547

Query: 545 FHQFVVPPILAFRRDCTYGELAAMRLPDDVQGLGNCEYTMSRGVVHACHAGGVVHHLEGW 560
           F  F   P    R+DC Y    AMR+P   Q + +CE  + R V+ A   GG+VH LEGW
Sbjct: 548 FVPFSHFPPNKLRKDCFYQSTPAMRVPKSAQNIDSCENWLGRRVMSAWKIGGIVHALEGW 607

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038879572.13.3e-30082.06very-long-chain aldehyde decarbonylase CER3 [Benincasa hispida][more]
XP_004149879.11.4e-29079.08very-long-chain aldehyde decarbonylase CER3 [Cucumis sativus] >KGN65271.1 hypoth... [more]
XP_023515806.17.8e-28677.50protein ECERIFERUM 3-like [Cucurbita pepo subsp. pepo][more]
KAG6589741.18.7e-28577.34Very-long-chain aldehyde decarbonylase CER3, partial [Cucurbita argyrosperma sub... [more]
XP_022921868.18.7e-28577.34protein ECERIFERUM 3-like isoform X1 [Cucurbita moschata] >KAG7023416.1 Protein ... [more]
Match NameE-valueIdentityDescription
Q8H1Z01.3e-20356.74Very-long-chain aldehyde decarbonylase CER3 OS=Arabidopsis thaliana OX=3702 GN=C... [more]
A2Z1F53.2e-18953.75Very-long-chain aldehyde decarbonylase GL1-1 OS=Oryza sativa subsp. indica OX=39... [more]
Q69PA83.2e-18953.75Very-long-chain aldehyde decarbonylase GL1-1 OS=Oryza sativa subsp. japonica OX=... [more]
Q67WQ73.4e-18352.46Very-long-chain aldehyde decarbonylase GL1-3 OS=Oryza sativa subsp. japonica OX=... [more]
Q6ETL86.4e-18253.15Very-long-chain aldehyde decarbonylase GL1-2 OS=Oryza sativa subsp. japonica OX=... [more]
Match NameE-valueIdentityDescription
A0A0A0LZ626.7e-29179.08Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G294020 PE=3 SV=1[more]
A0A6J1E5084.2e-28577.34protein ECERIFERUM 3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC1114300... [more]
A0A1S3B8V51.5e-28278.77protein ECERIFERUM 3 OS=Cucumis melo OX=3656 GN=LOC103487308 PE=3 SV=1[more]
A0A6J1JJW61.3e-28176.39protein ECERIFERUM 3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485764... [more]
A0A6J1BYY34.8e-28177.14protein ECERIFERUM 3 OS=Momordica charantia OX=3673 GN=LOC111007024 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G57800.19.4e-20556.74Fatty acid hydroxylase superfamily [more]
AT1G02205.21.3e-8130.45Fatty acid hydroxylase superfamily [more]
AT1G02205.31.9e-8030.45Fatty acid hydroxylase superfamily [more]
AT2G37700.13.4e-7731.85Fatty acid hydroxylase superfamily [more]
AT1G02190.11.5e-6930.21Fatty acid hydroxylase superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021940Uncharacterised domain Wax2, C-terminalPFAMPF12076Wax2_Ccoord: 407..558
e-value: 5.2E-54
score: 182.3
IPR006694Fatty acid hydroxylasePFAMPF04116FA_hydroxylasecoord: 129..263
e-value: 1.4E-14
score: 54.6
NoneNo IPR availablePANTHERPTHR11863:SF66VERY-LONG-CHAIN ALDEHYDE DECARBONYLASE CER3coord: 1..403
NoneNo IPR availablePANTHERPTHR11863STEROL DESATURASEcoord: 1..403
coord: 408..560
NoneNo IPR availablePANTHERPTHR11863:SF66VERY-LONG-CHAIN ALDEHYDE DECARBONYLASE CER3coord: 408..560

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10008214.1HG10008214.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016126 sterol biosynthetic process
biological_process GO:0008610 lipid biosynthetic process
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0000254 C-4 methylsterol oxidase activity
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016491 oxidoreductase activity