HG10006184 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10006184
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Descriptionervatamin-B-like
LocationChr07: 15445258 .. 15446616 (-)
RNA-Seq ExpressionHG10006184
SyntenyHG10006184
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTCATCATGAAATTTCTTATTGTTCTTGTTTTGATTGCTCTAACTTCTGGACTATGTGAAAGCTTTGAATTTGAAAGGAAGGAGTTAGAATCTGAAAAGAATCTATGGCATTTGTATAAGAGATGGAGTAGCCACCATAGAATCTCAAGGAATGGAAGAGAGATGTACAATCGTTTCAAGGTATTCAAAGATAATGCAAAGTATGTATCAAAAGTGAACCGAATGAACAAAACTTTCAAGTTGAAGCTGAACCAGTTTGCTGATATGTCCAATGATGAGTTCGTGAACTTATATACTAGCTCCAATATTACGTACTACAAAAACCTACATGCCAAGAAAATAGCAGAAGCTGGTGGGTGTGATGGTGGGTTCATGTATAAGAACGCTAAGGGTCTTCCACATTCCATTGATTGGAGGAAAAAAGGAGCTGTTAATGACATCAAAAACCAAGGCACAAGATGTGGTATAATTTATAATTTCGAATTTTGGCTTTATTATTCACCCTCATGATAACTAATCAATGATTTTGAAAATATATGTTTGCAGGAAGTTGTTGGGCGTTTGCAGCGGTGGCTGCAGTTGAAGGAATCCACCAAATCAAAACCAAGTCCCTATTATCTCTATCAGAGCAGGAGGTGGTCAATTGTGATTTTAGGGATGGAGGTTGCAATGGAGGATTCTATGACTCTGCTTTTGAATTCATTATGCAAAAGGATGGGATCACAACTGAGGAGAACTATCCCTACTATGCTGAAAATGATTACTGTCACTCACCGAGAGTGAGTTTTGAAGCAATTTCTAACTCTAAATTATATAATCATTTATCATTTTTGAAATTTTTTTAAGTTAATTAAGTAAATTATGTGTTTGATTTAATAGAGGAGTAATCCGAAAGTGACAATTGATGGGTATGAGAACGTACCTTCAAACAACGAGGATGCTTTGAAGAAGGCTGTGGCACACCAACCGGTAGCAGTAGCTATAGCGTCCAGTGGACGAGATTTCCAATTATACACTGGGGTAAGTAGTTTTGATCGTTTGAGTGCAAATTTGATTTTTGTAAACATTGTTAAGATTTTTGTAAACATTGTTAAAAGGATATTGTTTTGATCGTTTGAGTGTAAATTTGATTTTTGTAAACATTGTTAAAAGGATATTGTGAATTTGCAGGGAATCTTCACTGAAGACAAAGATTGTGGAAATCAAATTAATCACACAGTGGTGGTGGTTGGATACGGAACGGATGAAGACGGGACAGATTATTGGATCATAAGGAACTCATGGGGAGTTGGATGGGGAGCAGAAGGTTATATGAAGATGCAGCGAGGAGCGTGGAATCCAGTGGATTGGCAATGA

mRNA sequence

ATGGTCATCATGAAATTTCTTATTGTTCTTGTTTTGATTGCTCTAACTTCTGGACTATGTGAAAGCTTTGAATTTGAAAGGAAGGAGTTAGAATCTGAAAAGAATCTATGGCATTTGTATAAGAGATGGAGTAGCCACCATAGAATCTCAAGGAATGGAAGAGAGATGTACAATCGTTTCAAGGTATTCAAAGATAATGCAAAGTATGTATCAAAAGTGAACCGAATGAACAAAACTTTCAAGTTGAAGCTGAACCAGTTTGCTGATATGTCCAATGATGAGTTCGTGAACTTATATACTAGCTCCAATATTACGTACTACAAAAACCTACATGCCAAGAAAATAGCAGAAGCTGGTGGGTGTGATGGTGGGTTCATGTATAAGAACGCTAAGGGTCTTCCACATTCCATTGATTGGAGGAAAAAAGGAGCTGTTAATGACATCAAAAACCAAGGCACAAGATGTGGAAGTTGTTGGGCGTTTGCAGCGGTGGCTGCAGTTGAAGGAATCCACCAAATCAAAACCAAGTCCCTATTATCTCTATCAGAGCAGGAGGTGGTCAATTGTGATTTTAGGGATGGAGGTTGCAATGGAGGATTCTATGACTCTGCTTTTGAATTCATTATGCAAAAGGATGGGATCACAACTGAGGAGAACTATCCCTACTATGCTGAAAATGATTACTGTCACTCACCGAGAAGGAGTAATCCGAAAGTGACAATTGATGGGTATGAGAACGTACCTTCAAACAACGAGGATGCTTTGAAGAAGGCTGTGGCACACCAACCGGTAGCAGTAGCTATAGCGTCCAGTGGACGAGATTTCCAATTATACACTGGGGGAATCTTCACTGAAGACAAAGATTGTGGAAATCAAATTAATCACACAGTGGTGGTGGTTGGATACGGAACGGATGAAGACGGGACAGATTATTGGATCATAAGGAACTCATGGGGAGTTGGATGGGGAGCAGAAGGTTATATGAAGATGCAGCGAGGAGCGTGGAATCCAGTGGATTGGCAATGA

Coding sequence (CDS)

ATGGTCATCATGAAATTTCTTATTGTTCTTGTTTTGATTGCTCTAACTTCTGGACTATGTGAAAGCTTTGAATTTGAAAGGAAGGAGTTAGAATCTGAAAAGAATCTATGGCATTTGTATAAGAGATGGAGTAGCCACCATAGAATCTCAAGGAATGGAAGAGAGATGTACAATCGTTTCAAGGTATTCAAAGATAATGCAAAGTATGTATCAAAAGTGAACCGAATGAACAAAACTTTCAAGTTGAAGCTGAACCAGTTTGCTGATATGTCCAATGATGAGTTCGTGAACTTATATACTAGCTCCAATATTACGTACTACAAAAACCTACATGCCAAGAAAATAGCAGAAGCTGGTGGGTGTGATGGTGGGTTCATGTATAAGAACGCTAAGGGTCTTCCACATTCCATTGATTGGAGGAAAAAAGGAGCTGTTAATGACATCAAAAACCAAGGCACAAGATGTGGAAGTTGTTGGGCGTTTGCAGCGGTGGCTGCAGTTGAAGGAATCCACCAAATCAAAACCAAGTCCCTATTATCTCTATCAGAGCAGGAGGTGGTCAATTGTGATTTTAGGGATGGAGGTTGCAATGGAGGATTCTATGACTCTGCTTTTGAATTCATTATGCAAAAGGATGGGATCACAACTGAGGAGAACTATCCCTACTATGCTGAAAATGATTACTGTCACTCACCGAGAAGGAGTAATCCGAAAGTGACAATTGATGGGTATGAGAACGTACCTTCAAACAACGAGGATGCTTTGAAGAAGGCTGTGGCACACCAACCGGTAGCAGTAGCTATAGCGTCCAGTGGACGAGATTTCCAATTATACACTGGGGGAATCTTCACTGAAGACAAAGATTGTGGAAATCAAATTAATCACACAGTGGTGGTGGTTGGATACGGAACGGATGAAGACGGGACAGATTATTGGATCATAAGGAACTCATGGGGAGTTGGATGGGGAGCAGAAGGTTATATGAAGATGCAGCGAGGAGCGTGGAATCCAGTGGATTGGCAATGA

Protein sequence

MVIMKFLIVLVLIALTSGLCESFEFERKELESEKNLWHLYKRWSSHHRISRNGREMYNRFKVFKDNAKYVSKVNRMNKTFKLKLNQFADMSNDEFVNLYTSSNITYYKNLHAKKIAEAGGCDGGFMYKNAKGLPHSIDWRKKGAVNDIKNQGTRCGSCWAFAAVAAVEGIHQIKTKSLLSLSEQEVVNCDFRDGGCNGGFYDSAFEFIMQKDGITTEENYPYYAENDYCHSPRRSNPKVTIDGYENVPSNNEDALKKAVAHQPVAVAIASSGRDFQLYTGGIFTEDKDCGNQINHTVVVVGYGTDEDGTDYWIIRNSWGVGWGAEGYMKMQRGAWNPVDWQ
Homology
BLAST of HG10006184 vs. NCBI nr
Match: XP_038896226.1 (vignain-like [Benincasa hispida])

HSP 1 Score: 526.2 bits (1354), Expect = 2.1e-145
Identity = 258/336 (76.79%), Postives = 295/336 (87.80%), Query Frame = 0

Query: 3   IMKFLIV-LVLIALTSGLCESFEFERKELESEKNLWHLYKRWSSHHRISRNGREMYNRFK 62
           IMKFLIV LVLI LT GLCESF+FE KELESE++LWHLYKRWSSHHRI RNGREM+NRFK
Sbjct: 44  IMKFLIVPLVLIVLTFGLCESFKFEGKELESEESLWHLYKRWSSHHRILRNGREMHNRFK 103

Query: 63  VFKDNAKYVSKVNRMNKTFKLKLNQFADMSNDEFVNLYTSSNITYYKNLHAKKIAEAGGC 122
           VFK+NAKYV KVN+MNK+ KLKLNQFADMS+DEF+N + SSNITYYKNLHAKKI   GG 
Sbjct: 104 VFKENAKYVFKVNQMNKSLKLKLNQFADMSDDEFINTHVSSNITYYKNLHAKKIEALGGR 163

Query: 123 DGGFMYKNAKGLPHSIDWRKKGAVNDIKNQGTRCGSCWAFAAVAAVEGIHQIKTKSLLSL 182
            GGFMY++ + LP SIDWRKKGAV DIKNQG RCGSCWAFAAVA VEGI+QIKT  L+SL
Sbjct: 164 VGGFMYEHVEDLPSSIDWRKKGAVTDIKNQG-RCGSCWAFAAVAVVEGINQIKTNKLVSL 223

Query: 183 SEQEVVNCDFRDGGCNGGFYDSAFEFIMQKDGITTEENYPYYAENDYCHSPRRSNPKVTI 242
           SEQEVV+CD++DGGC GGFYDSAFEF+M+ +GIT EENYPYYAEN YC + ++ N +VTI
Sbjct: 224 SEQEVVDCDYKDGGCGGGFYDSAFEFMMENNGITIEENYPYYAENRYCLA-QKGNKRVTI 283

Query: 243 DGYENVPSNNEDALKKAVAHQPVAVAIASSGRDFQLYTGGIFTEDKDCGNQINHTVVVVG 302
           DGYENVP NNE+ALKKAVAHQPVAV+IA+SGR F+ Y+ G+FTE+  CG++I+HTVVVVG
Sbjct: 284 DGYENVPPNNENALKKAVAHQPVAVSIAASGRRFRYYSEGMFTENDYCGDRIDHTVVVVG 343

Query: 303 YGTDEDGTDYWIIRNSWGVGWGAEGYMKMQRGAWNP 338
           YGT+EDGTDYWII+NSWG  WG EGYMKMQRGA  P
Sbjct: 344 YGTEEDGTDYWIIKNSWGTNWGLEGYMKMQRGAQQP 377

BLAST of HG10006184 vs. NCBI nr
Match: XP_038885798.1 (vignain-like [Benincasa hispida])

HSP 1 Score: 516.5 bits (1329), Expect = 1.7e-142
Identity = 259/341 (75.95%), Postives = 295/341 (86.51%), Query Frame = 0

Query: 1   MVIMKFLIV-LVLIALTSGLCESFEFERKELESEKNLWHLYKRWSSHHRISRNGREMYNR 60
           + +MKFLIV LVLIAL  GLCESF FE  ELESE++LWHLYKRWSSHHRISRNG EM+ R
Sbjct: 66  ITMMKFLIVPLVLIALIFGLCESFTFEGNELESEESLWHLYKRWSSHHRISRNGWEMHYR 125

Query: 61  FKVFKDNAKYVSKVNRMNKTFKLKLNQFADMSNDEFVNLYTSSNITYYKNLHA-KKIAEA 120
           FKVFKDNAKYV KVN+MNK+ KLKLNQFADMS+DEF+N + +SNITYYKNLHA KK A  
Sbjct: 126 FKVFKDNAKYVFKVNQMNKSLKLKLNQFADMSDDEFINTHVNSNITYYKNLHAKKKEAVG 185

Query: 121 GGCDGGFMYKNAKGLPHSIDWRKKGAVNDIKNQGTRCGSCWAFAAVAAVEGIHQIKTKSL 180
           GG  GGFMY++A+ LP SIDWRKKGAV DIKNQG  CGSCWAFAAVA VEGIHQIKT  L
Sbjct: 186 GGRVGGFMYEHAEDLPSSIDWRKKGAVTDIKNQG-GCGSCWAFAAVAVVEGIHQIKTNKL 245

Query: 181 LSLSEQEVVNCDFRDGGCNGGFYDSAFEFIMQKDGITTEENYPYYAENDYCHSPRRSNPK 240
           +SLSEQEVV+CD+RDGGC GGFYDSAFEF+M+ +GITTEENYPYYAEN YC S +R+N +
Sbjct: 246 VSLSEQEVVDCDYRDGGCGGGFYDSAFEFMMKNNGITTEENYPYYAENRYCLS-QRANKR 305

Query: 241 VTIDGYENVPSNNEDALKKAVAHQPVAVAIASSGRDFQLYTGGIFTEDKDCGNQINHTVV 300
           VTIDGYENVP NNE+ALKKAVA+QPVAV+IA+SGR F+ Y+ G+FTE+  CG++I+HTVV
Sbjct: 306 VTIDGYENVPPNNENALKKAVANQPVAVSIAASGRRFRYYSEGMFTENDYCGDRIDHTVV 365

Query: 301 VVGYGTDEDGTDYWIIRNSWGVGWGAEGYMKMQRGAWNPVD 340
           VVGYGT+EDG DYWII+NSWG  WG EGYMKMQRGA  P D
Sbjct: 366 VVGYGTEEDGADYWIIKNSWGTNWGLEGYMKMQRGARQPED 404

BLAST of HG10006184 vs. NCBI nr
Match: XP_011658479.1 (ervatamin-B [Cucumis sativus] >KGN48503.1 hypothetical protein Csa_003803 [Cucumis sativus])

HSP 1 Score: 480.7 bits (1236), Expect = 1.0e-131
Identity = 243/338 (71.89%), Postives = 275/338 (81.36%), Query Frame = 0

Query: 1   MVIMKFLIV-LVLIALTSGLCESFEFERKELESEKNLWHLYKRWSSHHRISRNGREMYNR 60
           M +MKFLIV LVL+A +  +CESFE ERK+ ESEK+L  LYKRWSSHHRISRN  EM+NR
Sbjct: 1   MTVMKFLIVPLVLVAFSCNICESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHNR 60

Query: 61  FKVFKDNAKYVSKVNRMNKTFKLKLNQFADMSNDEFVNLYTSSNITYYKNLHAKKIAEAG 120
           FKVFK+NAK+V KVN M K+ KLKLNQFADMS+DEF N+Y SSNITYYK+LHAKKI   G
Sbjct: 61  FKVFKNNAKHVFKVNLMGKSLKLKLNQFADMSDDEFRNMY-SSNITYYKDLHAKKIEATG 120

Query: 121 GCDGGFMYKNAKGLPHSIDWRKKGAVNDIKNQGTRCGSCWAFAAVAAVEGIHQIKTKSLL 180
           G  GGFMY++A  +P SIDWRKKGAVN IKNQG RCGSCWAFAAVAAVE IHQIKT  L+
Sbjct: 121 GRIGGFMYEHANNIPSSIDWRKKGAVNAIKNQG-RCGSCWAFAAVAAVESIHQIKTNELV 180

Query: 181 SLSEQEVVNCDFRDGGCNGGFYDSAFEFIMQKDGITTEENYPYYAENDYCHSPRRSNPKV 240
           SLSE+EV++CD+RDGGC GGFY+SAFEF+M  DG+T E+NYPYY  N YC      N +V
Sbjct: 181 SLSEEEVLDCDYRDGGCRGGFYNSAFEFMMDNDGVTIEDNYPYYEGNGYCRRRGGRNKRV 240

Query: 241 TIDGYENVPSNNEDALKKAVAHQPVAVAIASSGRDFQLYTGGIFTEDKDCGNQINHTVVV 300
            IDGYENVP NNE AL KAVAHQPVAVAIAS G DF+ Y GG+FTE+  CG  I+HTVVV
Sbjct: 241 RIDGYENVPRNNEYALMKAVAHQPVAVAIASGGSDFKFYGGGMFTENDFCGFNIDHTVVV 300

Query: 301 VGYGTDEDGTDYWIIRNSWGVGWGAEGYMKMQRGAWNP 338
           VGYGTDEDG DYWIIRN +G  WG  GYMKMQRGA +P
Sbjct: 301 VGYGTDEDG-DYWIIRNQYGHRWGMNGYMKMQRGAHSP 335

BLAST of HG10006184 vs. NCBI nr
Match: XP_008454482.1 (PREDICTED: ervatamin-B-like [Cucumis melo])

HSP 1 Score: 463.8 bits (1192), Expect = 1.3e-126
Identity = 239/338 (70.71%), Postives = 267/338 (78.99%), Query Frame = 0

Query: 1   MVIMKFLIV-LVLIALTSGLCESFEFERKELESEKNLWHLYKRWSSHHRISRNGREMYNR 60
           M +MKFLIV LVLIA T  LCESFE ERK+ ESEK+L  LYKRWSSHHRISRN  EM+ R
Sbjct: 1   MAVMKFLIVPLVLIAFTFHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHKR 60

Query: 61  FKVFKDNAKYVSKVNRMNKTFKLKLNQFADMSNDEFVNLYTSSNITYYKNLHAKKIAEAG 120
           FKVFKDNAKYV K N M ++ KL+LNQFADMS+DEF +++  SNITYYKNLHAK      
Sbjct: 61  FKVFKDNAKYVFKKNHMGRSLKLQLNQFADMSDDEFSSIH-GSNITYYKNLHAK-----N 120

Query: 121 GCDGGFMYKNAKGLPHSIDWRKKGAVNDIKNQGTRCGSCWAFAAVAAVEGIHQIKTKSLL 180
           G  GGFMY++A  +P SIDWRKKGAVN IKNQG RCGSCWAFAAVAAVE IHQIKT  L+
Sbjct: 121 GRVGGFMYEHANDIPSSIDWRKKGAVNAIKNQG-RCGSCWAFAAVAAVESIHQIKTNELV 180

Query: 181 SLSEQEVVNCDFRDGGCNGGFYDSAFEFIMQKDGITTEENYPYYAENDYCHSPRRSNPKV 240
           SLSEQEVV+CD+RD GC GGFY+SAFEF+M+  GIT E+NYPYY  + YC      N +V
Sbjct: 181 SLSEQEVVDCDYRDSGCLGGFYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERV 240

Query: 241 TIDGYENVPSNNEDALKKAVAHQPVAVAIASSGRDFQLYTGGIFTEDKDCGNQINHTVVV 300
           TIDGYENVP NNE AL KAVAHQPVAVAIASSG DF+ Y  G+FTE   CG  I+HTVVV
Sbjct: 241 TIDGYENVPRNNEHALMKAVAHQPVAVAIASSGSDFRFYGQGMFTEQDFCGYNIDHTVVV 300

Query: 301 VGYGTDEDGTDYWIIRNSWGVGWGAEGYMKMQRGAWNP 338
           VGYGTDE+  DYWIIRN +G  WG  GYMKMQRGA NP
Sbjct: 301 VGYGTDEEDGDYWIIRNQYGTQWGMNGYMKMQRGARNP 331

BLAST of HG10006184 vs. NCBI nr
Match: XP_008454483.1 (PREDICTED: ervatamin-B-like [Cucumis melo])

HSP 1 Score: 460.3 bits (1183), Expect = 1.4e-125
Identity = 237/336 (70.54%), Postives = 267/336 (79.46%), Query Frame = 0

Query: 3   IMKFLIV-LVLIALTSGLCESFEFERKELESEKNLWHLYKRWSSHHRISRNGREMYNRFK 62
           +MKFLIV LVLIALTS LCESFE ERK+ ESEK+L  LYKRWSSHHRISRN  EM+ RFK
Sbjct: 4   VMKFLIVPLVLIALTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHKRFK 63

Query: 63  VFKDNAKYVSKVNRMNKTFKLKLNQFADMSNDEFVNLYTSSNITYYKNLHAKKIAEAGGC 122
           VFKDNAK+V K N M ++ KL+LNQFADMS+DEF +++  SNITYYKNLHAK      G 
Sbjct: 64  VFKDNAKHVFKKNHMGRSLKLQLNQFADMSDDEFSSIH-GSNITYYKNLHAKT-----GH 123

Query: 123 DGGFMYKNAKGLPHSIDWRKKGAVNDIKNQGTRCGSCWAFAAVAAVEGIHQIKTKSLLSL 182
            GGFMY++AK +P SIDWRKKGAVN IKNQG  CGSCWAFAAVAAVE IHQIKT  L+SL
Sbjct: 124 VGGFMYEHAKEIPSSIDWRKKGAVNAIKNQG-GCGSCWAFAAVAAVESIHQIKTNELVSL 183

Query: 183 SEQEVVNCDFRDGGCNGGFYDSAFEFIMQKDGITTEENYPYYAENDYCHSPRRSNPKVTI 242
           SEQEVV+CD+RDGGC GG Y+SAFEF+M+  GIT E+NYPYY  + YC      N +V I
Sbjct: 184 SEQEVVDCDYRDGGCRGGHYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVKI 243

Query: 243 DGYENVPSNNEDALKKAVAHQPVAVAIASSGRDFQLYTGGIFTEDKDCGNQINHTVVVVG 302
           DGYENVP NNE AL KAVAHQPVAVAIASSG DF+ Y  G+FTE   CG  I+HTVVVVG
Sbjct: 244 DGYENVPRNNEHALMKAVAHQPVAVAIASSGSDFRFYGQGMFTEQDFCGYNIDHTVVVVG 303

Query: 303 YGTDEDGTDYWIIRNSWGVGWGAEGYMKMQRGAWNP 338
           YG+DE+  DYWIIRN +G  WG  GYMKMQRGA NP
Sbjct: 304 YGSDEEDGDYWIIRNQYGTQWGMNGYMKMQRGARNP 332

BLAST of HG10006184 vs. ExPASy Swiss-Prot
Match: P12412 (Vignain OS=Vigna mungo OX=3915 PE=1 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 1.3e-97
Identity = 177/333 (53.15%), Postives = 236/333 (70.87%), Query Frame = 0

Query: 1   MVIMKFLIVLVLIALTSGLCESFEFERKELESEKNLWHLYKRWSSHHRISRNGREMYNRF 60
           M + K L V++ ++L  G+  SF+F  K+LESE++LW LY+RW SHH +SR+  E + RF
Sbjct: 1   MAMKKLLWVVLSLSLVLGVANSFDFHEKDLESEESLWDLYERWRSHHTVSRSLGEKHKRF 60

Query: 61  KVFKDNAKYVSKVNRMNKTFKLKLNQFADMSNDEFVNLYTSSNITYYKNLHAKKIAEAGG 120
            VFK N  +V   N+M+K +KLKLN+FADM+N EF + Y  S + ++K     +      
Sbjct: 61  NVFKANVMHVHNTNKMDKPYKLKLNKFADMTNHEFRSTYAGSKVNHHKMFRGSQHG---- 120

Query: 121 CDGGFMYKNAKGLPHSIDWRKKGAVNDIKNQGTRCGSCWAFAAVAAVEGIHQIKTKSLLS 180
             G FMY+    +P S+DWRKKGAV D+K+QG +CGSCWAF+ + AVEGI+QIKT  L+S
Sbjct: 121 -SGTFMYEKVGSVPASVDWRKKGAVTDVKDQG-QCGSCWAFSTIVAVEGINQIKTNKLVS 180

Query: 181 LSEQEVVNCDFRDG-GCNGGFYDSAFEFIMQKDGITTEENYPYYAENDYCHSPRRSNPKV 240
           LSEQE+V+CD  +  GCNGG  +SAFEFI QK GITTE NYPY A+   C   + ++  V
Sbjct: 181 LSEQELVDCDKEENQGCNGGLMESAFEFIKQKGGITTESNYPYTAQEGTCDESKVNDLAV 240

Query: 241 TIDGYENVPSNNEDALKKAVAHQPVAVAIASSGRDFQLYTGGIFTEDKDCGNQINHTVVV 300
           +IDG+ENVP N+E+AL KAVA+QPV+VAI + G DFQ Y+ G+FT   DC   +NH V +
Sbjct: 241 SIDGHENVPVNDENALLKAVANQPVSVAIDAGGSDFQFYSEGVFT--GDCNTDLNHGVAI 300

Query: 301 VGYGTDEDGTDYWIIRNSWGVGWGAEGYMKMQR 333
           VGYGT  DGT+YWI+RNSWG  WG +GY++MQR
Sbjct: 301 VGYGTTVDGTNYWIVRNSWGPEWGEQGYIRMQR 325

BLAST of HG10006184 vs. ExPASy Swiss-Prot
Match: P25803 (Vignain OS=Phaseolus vulgaris OX=3885 PE=2 SV=2)

HSP 1 Score: 353.2 bits (905), Expect = 3.3e-96
Identity = 177/333 (53.15%), Postives = 233/333 (69.97%), Query Frame = 0

Query: 1   MVIMKFLIVLVLIALTSGLCESFEFERKELESEKNLWHLYKRWSSHHRISRNGREMYNRF 60
           M   K L V++  +L  G+  SF+F  K+L SE++LW LY+RW SHH +SR+  E + RF
Sbjct: 1   MATKKLLWVVLSFSLVLGVANSFDFHDKDLASEESLWDLYERWRSHHTVSRSLGEKHKRF 60

Query: 61  KVFKDNAKYVSKVNRMNKTFKLKLNQFADMSNDEFVNLYTSSNITYYKNLHAKKIAEAGG 120
            VFK N  +V   N+M+K +KLKLN+FADM+N EF + Y  S +      H +       
Sbjct: 61  NVFKANLMHVHNTNKMDKPYKLKLNKFADMTNHEFRSTYAGSKVN-----HPRMFRGTPH 120

Query: 121 CDGGFMYKNAKGLPHSIDWRKKGAVNDIKNQGTRCGSCWAFAAVAAVEGIHQIKTKSLLS 180
            +G FMY+    +P S+DWRKKGAV D+K+QG +CGSCWAF+ V AVEGI+QIKT  L++
Sbjct: 121 ENGAFMYEKVVSVPPSVDWRKKGAVTDVKDQG-QCGSCWAFSTVVAVEGINQIKTNKLVA 180

Query: 181 LSEQEVVNCDFRDG-GCNGGFYDSAFEFIMQKDGITTEENYPYYAENDYCHSPRRSNPKV 240
           LSEQE+V+CD  +  GCNGG  +SAFEFI QK GITTE NYPY A+   C + + ++  V
Sbjct: 181 LSEQELVDCDKEENQGCNGGLMESAFEFIKQKGGITTESNYPYKAQEGTCDASKVNDLAV 240

Query: 241 TIDGYENVPSNNEDALKKAVAHQPVAVAIASSGRDFQLYTGGIFTEDKDCGNQINHTVVV 300
           +IDG+ENVP+N+EDAL KAVA+QPV+VAI + G DFQ Y+ G+FT   DC   +NH V +
Sbjct: 241 SIDGHENVPANDEDALLKAVANQPVSVAIDAGGSDFQFYSEGVFT--GDCSTDLNHGVAI 300

Query: 301 VGYGTDEDGTDYWIIRNSWGVGWGAEGYMKMQR 333
           VGYGT  DGT+YWI+RNSWG  WG  GY++MQR
Sbjct: 301 VGYGTTVDGTNYWIVRNSWGPEWGEHGYIRMQR 325

BLAST of HG10006184 vs. ExPASy Swiss-Prot
Match: O65039 (Vignain OS=Ricinus communis OX=3988 GN=CYSEP PE=1 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 3.3e-96
Identity = 176/334 (52.69%), Postives = 238/334 (71.26%), Query Frame = 0

Query: 3   IMKFLIVLVLIALTSGLCESFEFERKELESEKNLWHLYKRWSSHHRISRNGREMYNRFKV 62
           + KF+++ + +AL   + ESF+F  KELESE++LW LY+RW SHH +SR+  E   RF V
Sbjct: 1   MQKFILLALSLALVLAITESFDFHEKELESEESLWGLYERWRSHHTVSRSLHEKQKRFNV 60

Query: 63  FKDNAKYVSKVNRMNKTFKLKLNQFADMSNDEFVNLYTSSNITYYKNLHAKKIAEAGG-- 122
           FK NA +V   N+M+K +KLKLN+FADM+N EF N Y+ S + +++          GG  
Sbjct: 61  FKHNAMHVHNANKMDKPYKLKLNKFADMTNHEFRNTYSGSKVKHHRMFR-------GGPR 120

Query: 123 CDGGFMYKNAKGLPHSIDWRKKGAVNDIKNQGTRCGSCWAFAAVAAVEGIHQIKTKSLLS 182
            +G FMY+    +P S+DWRKKGAV  +K+QG +CGSCWAF+ + AVEGI+QIKT  L+S
Sbjct: 121 GNGTFMYEKVDTVPASVDWRKKGAVTSVKDQG-QCGSCWAFSTIVAVEGINQIKTNKLVS 180

Query: 183 LSEQEVVNCDF-RDGGCNGGFYDSAFEFIMQKDGITTEENYPYYAENDYCHSPRRSNPKV 242
           LSEQE+V+CD  ++ GCNGG  D AFEFI Q+ GITTE NYPY A +  C   + + P V
Sbjct: 181 LSEQELVDCDTDQNQGCNGGLMDYAFEFIKQRGGITTEANYPYEAYDGTCDVSKENAPAV 240

Query: 243 TIDGYENVPSNNEDALKKAVAHQPVAVAIASSGRDFQLYTGGIFTEDKDCGNQINHTVVV 302
           +IDG+ENVP N+E+AL KAVA+QPV+VAI + G DFQ Y+ G+FT    CG +++H V +
Sbjct: 241 SIDGHENVPENDENALLKAVANQPVSVAIDAGGSDFQFYSEGVFT--GSCGTELDHGVAI 300

Query: 303 VGYGTDEDGTDYWIIRNSWGVGWGAEGYMKMQRG 334
           VGYGT  DGT YW ++NSWG  WG +GY++M+RG
Sbjct: 301 VGYGTTIDGTKYWTVKNSWGPEWGEKGYIRMERG 324

BLAST of HG10006184 vs. ExPASy Swiss-Prot
Match: Q9FGR9 (KDEL-tailed cysteine endopeptidase CEP1 OS=Arabidopsis thaliana OX=3702 GN=CEP1 PE=1 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 2.2e-92
Identity = 171/332 (51.51%), Postives = 233/332 (70.18%), Query Frame = 0

Query: 3   IMKFLIVLVLIALTSGLCESFEFERKELESEKNLWHLYKRWSSHHRISRNGREMYNRFKV 62
           I+  L +L+++  T GL    +F  K++ESE +LW LY+RW SHH ++R+  E   RF V
Sbjct: 5   IVLALCMLMVLETTKGL----DFHNKDVESENSLWELYERWRSHHTVARSLEEKAKRFNV 64

Query: 63  FKDNAKYVSKVNRMNKTFKLKLNQFADMSNDEFVNLYTSSNITYYKNLHAKKIAEAGGCD 122
           FK N K++ + N+ +K++KLKLN+F DM+++EF   Y  SNI +++    +K A      
Sbjct: 65  FKHNVKHIHETNKKDKSYKLKLNKFGDMTSEEFRRTYAGSNIKHHRMFQGEKKATK---- 124

Query: 123 GGFMYKNAKGLPHSIDWRKKGAVNDIKNQGTRCGSCWAFAAVAAVEGIHQIKTKSLLSLS 182
             FMY N   LP S+DWRK GAV  +KNQG +CGSCWAF+ V AVEGI+QI+TK L SLS
Sbjct: 125 -SFMYANVNTLPTSVDWRKNGAVTPVKNQG-QCGSCWAFSTVVAVEGINQIRTKKLTSLS 184

Query: 183 EQEVVNCDF-RDGGCNGGFYDSAFEFIMQKDGITTEENYPYYAENDYCHSPRRSNPKVTI 242
           EQE+V+CD  ++ GCNGG  D AFEFI +K G+T+E  YPY A ++ C + + + P V+I
Sbjct: 185 EQELVDCDTNQNQGCNGGLMDLAFEFIKEKGGLTSELVYPYKASDETCDTNKENAPVVSI 244

Query: 243 DGYENVPSNNEDALKKAVAHQPVAVAIASSGRDFQLYTGGIFTEDKDCGNQINHTVVVVG 302
           DG+E+VP N+ED L KAVA+QPV+VAI + G DFQ Y+ G+FT    CG ++NH V VVG
Sbjct: 245 DGHEDVPKNSEDDLMKAVANQPVSVAIDAGGSDFQFYSEGVFT--GRCGTELNHGVAVVG 304

Query: 303 YGTDEDGTDYWIIRNSWGVGWGAEGYMKMQRG 334
           YGT  DGT YWI++NSWG  WG +GY++MQRG
Sbjct: 305 YGTTIDGTKYWIVKNSWGEEWGEKGYIRMQRG 324

BLAST of HG10006184 vs. ExPASy Swiss-Prot
Match: Q9STL5 (KDEL-tailed cysteine endopeptidase CEP3 OS=Arabidopsis thaliana OX=3702 GN=CEP3 PE=2 SV=1)

HSP 1 Score: 329.7 bits (844), Expect = 3.9e-89
Identity = 163/330 (49.39%), Postives = 224/330 (67.88%), Query Frame = 0

Query: 6   FLIVLVLIALTSGLCESFEFERKELESEKNLWHLYKRWSSHHRISRNGREMYNRFKVFKD 65
           F IVL+         + F+F+ KELE+E+N+W LY+RW  HH +SR   E   RF VF+ 
Sbjct: 4   FFIVLISFLSLLQASKGFDFDEKELETEENVWKLYERWRGHHSVSRASHEAIKRFNVFRH 63

Query: 66  NAKYVSKVNRMNKTFKLKLNQFADMSNDEFVNLYTSSNITYYKNLHAKKIAEAGGCDGGF 125
           N  +V + N+ NK +KLK+N+FAD+++ EF + Y  SN+ +++ L   K        GGF
Sbjct: 64  NVLHVHRTNKKNKPYKLKINRFADITHHEFRSSYAGSNVKHHRMLRGPKRG-----SGGF 123

Query: 126 MYKNAKGLPHSIDWRKKGAVNDIKNQGTRCGSCWAFAAVAAVEGIHQIKTKSLLSLSEQE 185
           MY+N   +P S+DWR+KGAV ++KNQ   CGSCWAF+ VAAVEGI++I+T  L+SLSEQE
Sbjct: 124 MYENVTRVPSSVDWREKGAVTEVKNQ-QDCGSCWAFSTVAAVEGINKIRTNKLVSLSEQE 183

Query: 186 VVNCDFRDG-GCNGGFYDSAFEFIMQKDGITTEENYPYYAEN-DYCHSPRRSNPKVTIDG 245
           +V+CD  +  GC GG  + AFEFI    GI TEE YPY + +  +C +       VTIDG
Sbjct: 184 LVDCDTEENQGCAGGLMEPAFEFIKNNGGIKTEETYPYDSSDVQFCRANSIGGETVTIDG 243

Query: 246 YENVPSNNEDALKKAVAHQPVAVAIASSGRDFQLYTGGIFTEDKDCGNQINHTVVVVGYG 305
           +E+VP N+E+ L KAVAHQPV+VAI +   DFQLY+ G+F    +CG Q+NH VV+VGYG
Sbjct: 244 HEHVPENDEEELLKAVAHQPVSVAIDAGSSDFQLYSEGVFI--GECGTQLNHGVVIVGYG 303

Query: 306 TDEDGTDYWIIRNSWGVGWGAEGYMKMQRG 334
             ++GT YWI+RNSWG  WG  GY++++RG
Sbjct: 304 ETKNGTKYWIVRNSWGPEWGEGGYVRIERG 325

BLAST of HG10006184 vs. ExPASy TrEMBL
Match: A0A0A0KGB1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G490180 PE=3 SV=1)

HSP 1 Score: 480.7 bits (1236), Expect = 5.0e-132
Identity = 243/338 (71.89%), Postives = 275/338 (81.36%), Query Frame = 0

Query: 1   MVIMKFLIV-LVLIALTSGLCESFEFERKELESEKNLWHLYKRWSSHHRISRNGREMYNR 60
           M +MKFLIV LVL+A +  +CESFE ERK+ ESEK+L  LYKRWSSHHRISRN  EM+NR
Sbjct: 1   MTVMKFLIVPLVLVAFSCNICESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHNR 60

Query: 61  FKVFKDNAKYVSKVNRMNKTFKLKLNQFADMSNDEFVNLYTSSNITYYKNLHAKKIAEAG 120
           FKVFK+NAK+V KVN M K+ KLKLNQFADMS+DEF N+Y SSNITYYK+LHAKKI   G
Sbjct: 61  FKVFKNNAKHVFKVNLMGKSLKLKLNQFADMSDDEFRNMY-SSNITYYKDLHAKKIEATG 120

Query: 121 GCDGGFMYKNAKGLPHSIDWRKKGAVNDIKNQGTRCGSCWAFAAVAAVEGIHQIKTKSLL 180
           G  GGFMY++A  +P SIDWRKKGAVN IKNQG RCGSCWAFAAVAAVE IHQIKT  L+
Sbjct: 121 GRIGGFMYEHANNIPSSIDWRKKGAVNAIKNQG-RCGSCWAFAAVAAVESIHQIKTNELV 180

Query: 181 SLSEQEVVNCDFRDGGCNGGFYDSAFEFIMQKDGITTEENYPYYAENDYCHSPRRSNPKV 240
           SLSE+EV++CD+RDGGC GGFY+SAFEF+M  DG+T E+NYPYY  N YC      N +V
Sbjct: 181 SLSEEEVLDCDYRDGGCRGGFYNSAFEFMMDNDGVTIEDNYPYYEGNGYCRRRGGRNKRV 240

Query: 241 TIDGYENVPSNNEDALKKAVAHQPVAVAIASSGRDFQLYTGGIFTEDKDCGNQINHTVVV 300
            IDGYENVP NNE AL KAVAHQPVAVAIAS G DF+ Y GG+FTE+  CG  I+HTVVV
Sbjct: 241 RIDGYENVPRNNEYALMKAVAHQPVAVAIASGGSDFKFYGGGMFTENDFCGFNIDHTVVV 300

Query: 301 VGYGTDEDGTDYWIIRNSWGVGWGAEGYMKMQRGAWNP 338
           VGYGTDEDG DYWIIRN +G  WG  GYMKMQRGA +P
Sbjct: 301 VGYGTDEDG-DYWIIRNQYGHRWGMNGYMKMQRGAHSP 335

BLAST of HG10006184 vs. ExPASy TrEMBL
Match: A0A1S3BYU0 (ervatamin-B-like OS=Cucumis melo OX=3656 GN=LOC103494878 PE=3 SV=1)

HSP 1 Score: 463.8 bits (1192), Expect = 6.3e-127
Identity = 239/338 (70.71%), Postives = 267/338 (78.99%), Query Frame = 0

Query: 1   MVIMKFLIV-LVLIALTSGLCESFEFERKELESEKNLWHLYKRWSSHHRISRNGREMYNR 60
           M +MKFLIV LVLIA T  LCESFE ERK+ ESEK+L  LYKRWSSHHRISRN  EM+ R
Sbjct: 1   MAVMKFLIVPLVLIAFTFHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHKR 60

Query: 61  FKVFKDNAKYVSKVNRMNKTFKLKLNQFADMSNDEFVNLYTSSNITYYKNLHAKKIAEAG 120
           FKVFKDNAKYV K N M ++ KL+LNQFADMS+DEF +++  SNITYYKNLHAK      
Sbjct: 61  FKVFKDNAKYVFKKNHMGRSLKLQLNQFADMSDDEFSSIH-GSNITYYKNLHAK-----N 120

Query: 121 GCDGGFMYKNAKGLPHSIDWRKKGAVNDIKNQGTRCGSCWAFAAVAAVEGIHQIKTKSLL 180
           G  GGFMY++A  +P SIDWRKKGAVN IKNQG RCGSCWAFAAVAAVE IHQIKT  L+
Sbjct: 121 GRVGGFMYEHANDIPSSIDWRKKGAVNAIKNQG-RCGSCWAFAAVAAVESIHQIKTNELV 180

Query: 181 SLSEQEVVNCDFRDGGCNGGFYDSAFEFIMQKDGITTEENYPYYAENDYCHSPRRSNPKV 240
           SLSEQEVV+CD+RD GC GGFY+SAFEF+M+  GIT E+NYPYY  + YC      N +V
Sbjct: 181 SLSEQEVVDCDYRDSGCLGGFYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERV 240

Query: 241 TIDGYENVPSNNEDALKKAVAHQPVAVAIASSGRDFQLYTGGIFTEDKDCGNQINHTVVV 300
           TIDGYENVP NNE AL KAVAHQPVAVAIASSG DF+ Y  G+FTE   CG  I+HTVVV
Sbjct: 241 TIDGYENVPRNNEHALMKAVAHQPVAVAIASSGSDFRFYGQGMFTEQDFCGYNIDHTVVV 300

Query: 301 VGYGTDEDGTDYWIIRNSWGVGWGAEGYMKMQRGAWNP 338
           VGYGTDE+  DYWIIRN +G  WG  GYMKMQRGA NP
Sbjct: 301 VGYGTDEEDGDYWIIRNQYGTQWGMNGYMKMQRGARNP 331

BLAST of HG10006184 vs. ExPASy TrEMBL
Match: A0A1S3BYQ3 (ervatamin-B-like OS=Cucumis melo OX=3656 GN=LOC103494879 PE=3 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 7.0e-126
Identity = 237/336 (70.54%), Postives = 267/336 (79.46%), Query Frame = 0

Query: 3   IMKFLIV-LVLIALTSGLCESFEFERKELESEKNLWHLYKRWSSHHRISRNGREMYNRFK 62
           +MKFLIV LVLIALTS LCESFE ERK+ ESEK+L  LYKRWSSHHRISRN  EM+ RFK
Sbjct: 4   VMKFLIVPLVLIALTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHKRFK 63

Query: 63  VFKDNAKYVSKVNRMNKTFKLKLNQFADMSNDEFVNLYTSSNITYYKNLHAKKIAEAGGC 122
           VFKDNAK+V K N M ++ KL+LNQFADMS+DEF +++  SNITYYKNLHAK      G 
Sbjct: 64  VFKDNAKHVFKKNHMGRSLKLQLNQFADMSDDEFSSIH-GSNITYYKNLHAKT-----GH 123

Query: 123 DGGFMYKNAKGLPHSIDWRKKGAVNDIKNQGTRCGSCWAFAAVAAVEGIHQIKTKSLLSL 182
            GGFMY++AK +P SIDWRKKGAVN IKNQG  CGSCWAFAAVAAVE IHQIKT  L+SL
Sbjct: 124 VGGFMYEHAKEIPSSIDWRKKGAVNAIKNQG-GCGSCWAFAAVAAVESIHQIKTNELVSL 183

Query: 183 SEQEVVNCDFRDGGCNGGFYDSAFEFIMQKDGITTEENYPYYAENDYCHSPRRSNPKVTI 242
           SEQEVV+CD+RDGGC GG Y+SAFEF+M+  GIT E+NYPYY  + YC      N +V I
Sbjct: 184 SEQEVVDCDYRDGGCRGGHYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVKI 243

Query: 243 DGYENVPSNNEDALKKAVAHQPVAVAIASSGRDFQLYTGGIFTEDKDCGNQINHTVVVVG 302
           DGYENVP NNE AL KAVAHQPVAVAIASSG DF+ Y  G+FTE   CG  I+HTVVVVG
Sbjct: 244 DGYENVPRNNEHALMKAVAHQPVAVAIASSGSDFRFYGQGMFTEQDFCGYNIDHTVVVVG 303

Query: 303 YGTDEDGTDYWIIRNSWGVGWGAEGYMKMQRGAWNP 338
           YG+DE+  DYWIIRN +G  WG  GYMKMQRGA NP
Sbjct: 304 YGSDEEDGDYWIIRNQYGTQWGMNGYMKMQRGARNP 332

BLAST of HG10006184 vs. ExPASy TrEMBL
Match: A0A5A7TM64 (Ervatamin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G003430 PE=3 SV=1)

HSP 1 Score: 458.8 bits (1179), Expect = 2.0e-125
Identity = 236/336 (70.24%), Postives = 266/336 (79.17%), Query Frame = 0

Query: 3   IMKFLIV-LVLIALTSGLCESFEFERKELESEKNLWHLYKRWSSHHRISRNGREMYNRFK 62
           +MKFLIV  VLIALTS LCESFE ERK+ ESEK+L  LYKRWSSHHRISRN  EM+ RFK
Sbjct: 4   VMKFLIVPFVLIALTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHKRFK 63

Query: 63  VFKDNAKYVSKVNRMNKTFKLKLNQFADMSNDEFVNLYTSSNITYYKNLHAKKIAEAGGC 122
           VFKDNAK+V K N M ++ KL+LNQFADMS+DEF +++  SNITYYKNLHAK      G 
Sbjct: 64  VFKDNAKHVFKKNHMGRSLKLQLNQFADMSDDEFSSIH-GSNITYYKNLHAKT-----GH 123

Query: 123 DGGFMYKNAKGLPHSIDWRKKGAVNDIKNQGTRCGSCWAFAAVAAVEGIHQIKTKSLLSL 182
            GGFMY++AK +P SIDWRKKGAVN IKNQG  CGSCWAFAAVAAVE IHQIKT  L+SL
Sbjct: 124 VGGFMYEHAKEIPSSIDWRKKGAVNAIKNQG-GCGSCWAFAAVAAVESIHQIKTNELVSL 183

Query: 183 SEQEVVNCDFRDGGCNGGFYDSAFEFIMQKDGITTEENYPYYAENDYCHSPRRSNPKVTI 242
           SEQEVV+CD+RDGGC GG Y+SAFEF+M+  GIT E+NYPYY  + YC      N +V I
Sbjct: 184 SEQEVVDCDYRDGGCRGGHYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVKI 243

Query: 243 DGYENVPSNNEDALKKAVAHQPVAVAIASSGRDFQLYTGGIFTEDKDCGNQINHTVVVVG 302
           DGYENVP NNE AL KAVAHQPVAVAIASSG DF+ Y  G+FTE   CG  I+HTVVVVG
Sbjct: 244 DGYENVPRNNEHALMKAVAHQPVAVAIASSGSDFRFYGQGMFTEQDFCGYNIDHTVVVVG 303

Query: 303 YGTDEDGTDYWIIRNSWGVGWGAEGYMKMQRGAWNP 338
           YG+DE+  DYWIIRN +G  WG  GYMKMQRGA NP
Sbjct: 304 YGSDEEDGDYWIIRNQYGTQWGMNGYMKMQRGARNP 332

BLAST of HG10006184 vs. ExPASy TrEMBL
Match: A0A5D3D043 (Ervatamin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001320 PE=3 SV=1)

HSP 1 Score: 458.0 bits (1177), Expect = 3.5e-125
Identity = 239/340 (70.29%), Postives = 267/340 (78.53%), Query Frame = 0

Query: 1   MVIMKFLIV-LVLIALTSGLCESFEFERKELESEKNLWHLYKRWSSHHRISRNGREMYNR 60
           M +MKFLIV LVLIA T  LCESFE ERK+ ESEK+L  LYKRWSSHHRISRN  EM+ R
Sbjct: 1   MAVMKFLIVPLVLIAFTFHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHKR 60

Query: 61  FKVFKDNAKYVSKVNRMNKTFKLKLNQFADMSNDEFVNLYTSSNITYYKNLHAKKIAEAG 120
           FKVFKDNAKYV K N M ++ KL+LNQFADMS+DEF +++  SNITYYKNLHAK      
Sbjct: 61  FKVFKDNAKYVFKKNHMGRSLKLQLNQFADMSDDEFSSIH-GSNITYYKNLHAK-----N 120

Query: 121 GCDGGFMYKNAKGLPHSIDWRKKGAVNDIKNQGTRCGSCWAFAAVAAVEGIHQIKTKSLL 180
           G  GGFMY++A  +P SIDWRKKGAVN IKNQG RCGSCWAFAAVAAVE IHQIKT  L+
Sbjct: 121 GRVGGFMYEHANDIPSSIDWRKKGAVNAIKNQG-RCGSCWAFAAVAAVESIHQIKTNELV 180

Query: 181 SLSEQEVVNCDFRDGGCNGGFYDSAFEFIMQKDGITTEENYPYYAENDYCHSPRRSNPKV 240
           SLSEQEVV+CD+RD GC GGFY+SAFEF+M+  GIT E+NYPYY  + YC      N +V
Sbjct: 181 SLSEQEVVDCDYRDSGCLGGFYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERV 240

Query: 241 TIDGYENVPSNNEDALKKAVAHQPVAVAIASSGR--DFQLYTGGIFTEDKDCGNQINHTV 300
           TIDGYENVP NNE AL KAVAHQPVAVAIASSGR  +F  Y  G+FTE   CG  I+HTV
Sbjct: 241 TIDGYENVPRNNEHALMKAVAHQPVAVAIASSGRILNFDNYLQGMFTEQDFCGYNIDHTV 300

Query: 301 VVVGYGTDEDGTDYWIIRNSWGVGWGAEGYMKMQRGAWNP 338
           VVVGYGTDE+  DYWIIRN +G  WG  GYMKMQRGA NP
Sbjct: 301 VVVGYGTDEEDGDYWIIRNQYGTQWGMNGYMKMQRGARNP 333

BLAST of HG10006184 vs. TAIR 10
Match: AT5G50260.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 340.5 bits (872), Expect = 1.6e-93
Identity = 171/332 (51.51%), Postives = 233/332 (70.18%), Query Frame = 0

Query: 3   IMKFLIVLVLIALTSGLCESFEFERKELESEKNLWHLYKRWSSHHRISRNGREMYNRFKV 62
           I+  L +L+++  T GL    +F  K++ESE +LW LY+RW SHH ++R+  E   RF V
Sbjct: 5   IVLALCMLMVLETTKGL----DFHNKDVESENSLWELYERWRSHHTVARSLEEKAKRFNV 64

Query: 63  FKDNAKYVSKVNRMNKTFKLKLNQFADMSNDEFVNLYTSSNITYYKNLHAKKIAEAGGCD 122
           FK N K++ + N+ +K++KLKLN+F DM+++EF   Y  SNI +++    +K A      
Sbjct: 65  FKHNVKHIHETNKKDKSYKLKLNKFGDMTSEEFRRTYAGSNIKHHRMFQGEKKATK---- 124

Query: 123 GGFMYKNAKGLPHSIDWRKKGAVNDIKNQGTRCGSCWAFAAVAAVEGIHQIKTKSLLSLS 182
             FMY N   LP S+DWRK GAV  +KNQG +CGSCWAF+ V AVEGI+QI+TK L SLS
Sbjct: 125 -SFMYANVNTLPTSVDWRKNGAVTPVKNQG-QCGSCWAFSTVVAVEGINQIRTKKLTSLS 184

Query: 183 EQEVVNCDF-RDGGCNGGFYDSAFEFIMQKDGITTEENYPYYAENDYCHSPRRSNPKVTI 242
           EQE+V+CD  ++ GCNGG  D AFEFI +K G+T+E  YPY A ++ C + + + P V+I
Sbjct: 185 EQELVDCDTNQNQGCNGGLMDLAFEFIKEKGGLTSELVYPYKASDETCDTNKENAPVVSI 244

Query: 243 DGYENVPSNNEDALKKAVAHQPVAVAIASSGRDFQLYTGGIFTEDKDCGNQINHTVVVVG 302
           DG+E+VP N+ED L KAVA+QPV+VAI + G DFQ Y+ G+FT    CG ++NH V VVG
Sbjct: 245 DGHEDVPKNSEDDLMKAVANQPVSVAIDAGGSDFQFYSEGVFT--GRCGTELNHGVAVVG 304

Query: 303 YGTDEDGTDYWIIRNSWGVGWGAEGYMKMQRG 334
           YGT  DGT YWI++NSWG  WG +GY++MQRG
Sbjct: 305 YGTTIDGTKYWIVKNSWGEEWGEKGYIRMQRG 324

BLAST of HG10006184 vs. TAIR 10
Match: AT3G48350.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 329.7 bits (844), Expect = 2.7e-90
Identity = 163/330 (49.39%), Postives = 224/330 (67.88%), Query Frame = 0

Query: 6   FLIVLVLIALTSGLCESFEFERKELESEKNLWHLYKRWSSHHRISRNGREMYNRFKVFKD 65
           F IVL+         + F+F+ KELE+E+N+W LY+RW  HH +SR   E   RF VF+ 
Sbjct: 4   FFIVLISFLSLLQASKGFDFDEKELETEENVWKLYERWRGHHSVSRASHEAIKRFNVFRH 63

Query: 66  NAKYVSKVNRMNKTFKLKLNQFADMSNDEFVNLYTSSNITYYKNLHAKKIAEAGGCDGGF 125
           N  +V + N+ NK +KLK+N+FAD+++ EF + Y  SN+ +++ L   K        GGF
Sbjct: 64  NVLHVHRTNKKNKPYKLKINRFADITHHEFRSSYAGSNVKHHRMLRGPKRG-----SGGF 123

Query: 126 MYKNAKGLPHSIDWRKKGAVNDIKNQGTRCGSCWAFAAVAAVEGIHQIKTKSLLSLSEQE 185
           MY+N   +P S+DWR+KGAV ++KNQ   CGSCWAF+ VAAVEGI++I+T  L+SLSEQE
Sbjct: 124 MYENVTRVPSSVDWREKGAVTEVKNQ-QDCGSCWAFSTVAAVEGINKIRTNKLVSLSEQE 183

Query: 186 VVNCDFRDG-GCNGGFYDSAFEFIMQKDGITTEENYPYYAEN-DYCHSPRRSNPKVTIDG 245
           +V+CD  +  GC GG  + AFEFI    GI TEE YPY + +  +C +       VTIDG
Sbjct: 184 LVDCDTEENQGCAGGLMEPAFEFIKNNGGIKTEETYPYDSSDVQFCRANSIGGETVTIDG 243

Query: 246 YENVPSNNEDALKKAVAHQPVAVAIASSGRDFQLYTGGIFTEDKDCGNQINHTVVVVGYG 305
           +E+VP N+E+ L KAVAHQPV+VAI +   DFQLY+ G+F    +CG Q+NH VV+VGYG
Sbjct: 244 HEHVPENDEEELLKAVAHQPVSVAIDAGSSDFQLYSEGVFI--GECGTQLNHGVVIVGYG 303

Query: 306 TDEDGTDYWIIRNSWGVGWGAEGYMKMQRG 334
             ++GT YWI+RNSWG  WG  GY++++RG
Sbjct: 304 ETKNGTKYWIVRNSWGPEWGEGGYVRIERG 325

BLAST of HG10006184 vs. TAIR 10
Match: AT3G48340.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 317.0 bits (811), Expect = 1.8e-86
Identity = 165/332 (49.70%), Postives = 227/332 (68.37%), Query Frame = 0

Query: 2   VIMKFLIVLVLIALTSGLCESFEFERKELESEKNLWHLYKRWSSHHRISRNGREMYNRFK 61
           +++ FL  LV++    G    F+++ KE+ESE+ L  LY RW SHH + R+  E   RF 
Sbjct: 4   LLLIFLFSLVILQTACG----FDYDDKEIESEEGLSTLYDRWRSHHSVPRSLNEREKRFN 63

Query: 62  VFKDNAKYVSKVNRMNKTFKLKLNQFADMSNDEFVNLYTSSNITYYKNLHAKKIAEAGGC 121
           VF+ N  +V   N+ N+++KLKLN+FAD++ +EF N YT SNI +++ L   K    G  
Sbjct: 64  VFRHNVMHVHNTNKKNRSYKLKLNKFADLTINEFKNAYTGSNIKHHRMLQGPK---RGSK 123

Query: 122 DGGFMYKNAKGLPHSIDWRKKGAVNDIKNQGTRCGSCWAFAAVAAVEGIHQIKTKSLLSL 181
              + ++N   LP S+DWRKKGAV +IKNQG +CGSCWAF+ VAAVEGI++IKT  L+SL
Sbjct: 124 QFMYDHENLSKLPSSVDWRKKGAVTEIKNQG-KCGSCWAFSTVAAVEGINKIKTNKLVSL 183

Query: 182 SEQEVVNCDFRDG-GCNGGFYDSAFEFIMQKDGITTEENYPYYAENDYCHSPRRSNPKVT 241
           SEQE+V+CD +   GCNGG  + AFEFI +  GITTE++YPY   +  C + + +   VT
Sbjct: 184 SEQELVDCDTKQNEGCNGGLMEIAFEFIKKNGGITTEDSYPYEGIDGKCDASKDNGVLVT 243

Query: 242 IDGYENVPSNNEDALKKAVAHQPVAVAIASSGRDFQLYTGGIFTEDKDCGNQINHTVVVV 301
           IDG+E+VP N+E+AL KAVA+QPV+VAI +   DFQ Y+ G+FT    CG ++NH V  V
Sbjct: 244 IDGHEDVPENDENALLKAVANQPVSVAIDAGSSDFQFYSEGVFT--GSCGTELNHGVAAV 303

Query: 302 GYGTDEDGTDYWIIRNSWGVGWGAEGYMKMQR 333
           GYG+ E G  YWI+RNSWG  WG  GY+K++R
Sbjct: 304 GYGS-ERGKKYWIVRNSWGAEWGEGGYIKIER 324

BLAST of HG10006184 vs. TAIR 10
Match: AT3G19390.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 288.1 bits (736), Expect = 9.2e-78
Identity = 156/327 (47.71%), Postives = 222/327 (67.89%), Query Frame = 0

Query: 10  LVLIALTSGLCESFEFERKELESEKNLWHLYKRWSSHHRISRNG-REMYNRFKVFKDNAK 69
           ++LI+L+ G   + E  R E E+ +    +Y+RW   +R + NG  E   RF++FKDN K
Sbjct: 17  VLLISLSLGSVTATETTRNEAEARR----MYERWLVENRKNYNGLGEKERRFEIFKDNLK 76

Query: 70  YVSKVNRM-NKTFKLKLNQFADMSNDEFVNLYTSSNITYYKNLHAKKIAEAGGCDGGFMY 129
           +V + + + N+T+++ L +FAD++NDEF  +Y  S       +   ++   G     ++Y
Sbjct: 77  FVEEHSSIPNRTYEVGLTRFADLTNDEFRAIYLRS------KMERTRVPVKG---EKYLY 136

Query: 130 KNAKGLPHSIDWRKKGAVNDIKNQGTRCGSCWAFAAVAAVEGIHQIKTKSLLSLSEQEVV 189
           K    LP +IDWR KGAVN +K+QG+ CGSCWAF+A+ AVEGI+QIKT  L+SLSEQE+V
Sbjct: 137 KVGDSLPDAIDWRAKGAVNPVKDQGS-CGSCWAFSAIGAVEGINQIKTGELISLSEQELV 196

Query: 190 NCDFR-DGGCNGGFYDSAFEFIMQKDGITTEENYPYYAEN-DYCHSPRRSNPKVTIDGYE 249
           +CD   + GC GG  D AF+FI++  GI TEE+YPY A + + C+S +++   VTIDGYE
Sbjct: 197 DCDTSYNDGCGGGLMDYAFKFIIENGGIDTEEDYPYIATDVNVCNSDKKNTRVVTIDGYE 256

Query: 250 NVPSNNEDALKKAVAHQPVAVAIASSGRDFQLYTGGIFTEDKDCGNQINHTVVVVGYGTD 309
           +VP N+E +LKKA+A+QP++VAI + GR FQLYT G+FT    CG  ++H VV VGYG+ 
Sbjct: 257 DVPQNDEKSLKKALANQPISVAIEAGGRAFQLYTSGVFT--GTCGTSLDHGVVAVGYGS- 316

Query: 310 EDGTDYWIIRNSWGVGWGAEGYMKMQR 333
           E G DYWI+RNSWG  WG  GY K++R
Sbjct: 317 EGGQDYWIVRNSWGSNWGESGYFKLER 326

BLAST of HG10006184 vs. TAIR 10
Match: AT5G45890.1 (senescence-associated gene 12 )

HSP 1 Score: 279.6 bits (714), Expect = 3.3e-75
Identity = 153/335 (45.67%), Postives = 215/335 (64.18%), Query Frame = 0

Query: 4   MKFLIVLVLIALTSGLCESFEFERKELESEKNLWHLYKRW-SSHHRISRNGREMYNRFKV 63
           +K + + + +A+ S  C S    R  L++E  +   +  W + H R+  + +E  NR+ V
Sbjct: 3   LKHMQIFLFVAIFSSFCFSITLSR-PLDNELIMQKRHIEWMTKHGRVYADVKEENNRYVV 62

Query: 64  FKDNAKYVSKVNRM--NKTFKLKLNQFADMSNDEFVNLYTSSNITYYKNLHAKKIAEAGG 123
           FK+N + +  +N +   +TFKL +NQFAD++NDEF ++YT      +K + A   +++  
Sbjct: 63  FKNNVERIEHLNSIPAGRTFKLAVNQFADLTNDEFRSMYTG-----FKGVSALS-SQSQT 122

Query: 124 CDGGFMYKNAK--GLPHSIDWRKKGAVNDIKNQGTRCGSCWAFAAVAAVEGIHQIKTKSL 183
               F Y+N     LP S+DWRKKGAV  IKNQG+ CG CWAF+AVAA+EG  QIK   L
Sbjct: 123 KMSPFRYQNVSSGALPVSVDWRKKGAVTPIKNQGS-CGCCWAFSAVAAIEGATQIKKGKL 182

Query: 184 LSLSEQEVVNCDFRDGGCNGGFYDSAFEFIMQKDGITTEENYPYYAENDYCHSPRRSNPK 243
           +SLSEQ++V+CD  D GC GG  D+AFE I    G+TTE NYPY  E+  C+S +++NPK
Sbjct: 183 ISLSEQQLVDCDTNDFGCEGGLMDTAFEHIKATGGLTTESNYPYKGEDATCNS-KKTNPK 242

Query: 244 VT-IDGYENVPSNNEDALKKAVAHQPVAVAIASSGRDFQLYTGGIFTEDKDCGNQINHTV 303
            T I GYE+VP N+E AL KAVAHQPV+V I   G DFQ Y+ G+FT   +C   ++H V
Sbjct: 243 ATSITGYEDVPVNDEQALMKAVAHQPVSVGIEGGGFDFQFYSSGVFT--GECTTYLDHAV 302

Query: 304 VVVGYGTDEDGTDYWIIRNSWGVGWGAEGYMKMQR 333
             +GYG   +G+ YWII+NSWG  WG  GYM++Q+
Sbjct: 303 TAIGYGESTNGSKYWIIKNSWGTKWGESGYMRIQK 326

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038896226.12.1e-14576.79vignain-like [Benincasa hispida][more]
XP_038885798.11.7e-14275.95vignain-like [Benincasa hispida][more]
XP_011658479.11.0e-13171.89ervatamin-B [Cucumis sativus] >KGN48503.1 hypothetical protein Csa_003803 [Cucum... [more]
XP_008454482.11.3e-12670.71PREDICTED: ervatamin-B-like [Cucumis melo][more]
XP_008454483.11.4e-12570.54PREDICTED: ervatamin-B-like [Cucumis melo][more]
Match NameE-valueIdentityDescription
P124121.3e-9753.15Vignain OS=Vigna mungo OX=3915 PE=1 SV=1[more]
P258033.3e-9653.15Vignain OS=Phaseolus vulgaris OX=3885 PE=2 SV=2[more]
O650393.3e-9652.69Vignain OS=Ricinus communis OX=3988 GN=CYSEP PE=1 SV=1[more]
Q9FGR92.2e-9251.51KDEL-tailed cysteine endopeptidase CEP1 OS=Arabidopsis thaliana OX=3702 GN=CEP1 ... [more]
Q9STL53.9e-8949.39KDEL-tailed cysteine endopeptidase CEP3 OS=Arabidopsis thaliana OX=3702 GN=CEP3 ... [more]
Match NameE-valueIdentityDescription
A0A0A0KGB15.0e-13271.89Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G490180 PE=3 SV=1[more]
A0A1S3BYU06.3e-12770.71ervatamin-B-like OS=Cucumis melo OX=3656 GN=LOC103494878 PE=3 SV=1[more]
A0A1S3BYQ37.0e-12670.54ervatamin-B-like OS=Cucumis melo OX=3656 GN=LOC103494879 PE=3 SV=1[more]
A0A5A7TM642.0e-12570.24Ervatamin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G0034... [more]
A0A5D3D0433.5e-12570.29Ervatamin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001... [more]
Match NameE-valueIdentityDescription
AT5G50260.11.6e-9351.51Cysteine proteinases superfamily protein [more]
AT3G48350.12.7e-9049.39Cysteine proteinases superfamily protein [more]
AT3G48340.11.8e-8649.70Cysteine proteinases superfamily protein [more]
AT3G19390.19.2e-7847.71Granulin repeat cysteine protease family protein [more]
AT5G45890.13.3e-7545.67senescence-associated gene 12 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000668Peptidase C1A, papain C-terminalPRINTSPR00705PAPAINcoord: 311..317
score: 69.12
coord: 152..167
score: 51.87
coord: 295..305
score: 53.51
IPR000668Peptidase C1A, papain C-terminalSMARTSM00645pept_c1coord: 133..338
e-value: 2.5E-95
score: 332.7
IPR000668Peptidase C1A, papain C-terminalPFAMPF00112Peptidase_C1coord: 133..334
e-value: 7.8E-73
score: 245.2
IPR013201Cathepsin propeptide inhibitor domain (I29)SMARTSM00848Inhibitor_I29_2coord: 40..95
e-value: 9.0E-13
score: 58.4
IPR013201Cathepsin propeptide inhibitor domain (I29)PFAMPF08246Inhibitor_I29coord: 40..95
e-value: 5.9E-11
score: 42.6
NoneNo IPR availableGENE3D3.90.70.10Cysteine proteinasescoord: 14..338
e-value: 1.4E-109
score: 368.8
NoneNo IPR availablePANTHERPTHR12411CYSTEINE PROTEASE FAMILY C1-RELATEDcoord: 20..334
NoneNo IPR availablePANTHERPTHR12411:SF706KDEL-TAILED CYSTEINE ENDOPEPTIDASE CEP1coord: 20..334
IPR025661Cysteine peptidase, asparagine active sitePROSITEPS00640THIOL_PROTEASE_ASNcoord: 311..330
IPR039417Papain-like cysteine endopeptidaseCDDcd02248Peptidase_C1Acoord: 134..334
e-value: 2.71614E-96
score: 282.59
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILY54001Cysteine proteinasescoord: 37..335

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10006184.1HG10006184.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051603 proteolysis involved in cellular protein catabolic process
biological_process GO:0006508 proteolysis
cellular_component GO:0005615 extracellular space
cellular_component GO:0005764 lysosome
molecular_function GO:0004197 cysteine-type endopeptidase activity
molecular_function GO:0008234 cysteine-type peptidase activity