HG10000872 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10000872 vs. NCBI nr
Match: XP_038878641.1 (NPC intracellular cholesterol transporter 1-like [Benincasa hispida])

HSP 1 Score: 1385.9 bits (3586), Expect = 0.0e+00
Identity = 732/809 (90.48%), Postives = 749/809 (92.58%), Query Frame = 0

Query: 1    MDPENFDDYGGVEHAEYCFQHYASTETCFSAFKAPLDPSTSLGGFVGNNYSEASAFVVTY 60
            M+PENFDDYGGVEHAEYCFQHY STETCFSAFKAPLDPSTSLGGFVGNNYSEASAFVVTY
Sbjct: 516  MNPENFDDYGGVEHAEYCFQHYTSTETCFSAFKAPLDPSTSLGGFVGNNYSEASAFVVTY 575

Query: 61   PVNNAIDAVGNENGKAIAWEKAFVKLAKEELLPLVQSRNLTLSFSSESSIEEELKRESTA 120
            PVNNA+D+VGNENGKAIAWEKAFVKLAKEELLPLVQSRNLTLSFSSESSIEEELKRESTA
Sbjct: 576  PVNNAMDSVGNENGKAIAWEKAFVKLAKEELLPLVQSRNLTLSFSSESSIEEELKRESTA 635

Query: 121  DILTIA----------------------------VLLGLSGVILVVLSVLGSVGFFSAIG 180
            DILTIA                            VLLGLSGVILV+LSVLGSVGFFSAIG
Sbjct: 636  DILTIAVSYLVMFAYISVALGDSNISSSFYLSSKVLLGLSGVILVLLSVLGSVGFFSAIG 695

Query: 181  VKSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQPYELTLEERISSALVEVGPSITLASLS 240
            VKSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQPYELTLEERISSALVEVGPSITLASLS
Sbjct: 696  VKSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQPYELTLEERISSALVEVGPSITLASLS 755

Query: 241  EILAFAVGTFVPMPACRVFSMFAALAVLLDFILQLSAFVALIVFDILRAEDHRVDCFPCI 300
            EILAFAVG FVPMPACRVFSMFAALAVLLDFILQLSAFVALIVFDILRAEDHRVDCFPCI
Sbjct: 756  EILAFAVGAFVPMPACRVFSMFAALAVLLDFILQLSAFVALIVFDILRAEDHRVDCFPCI 815

Query: 301  KVPPHSDELNQGFNQGRPGLLSRYMKDVHAPLLGLWGVKIVVVTIFVGLTLGSIALSTKI 360
            KVPPHSDE NQGFNQGRPGLLSRYMKDVHAPLLGLWGVKIVVV IFVGLTLGSIALSTKI
Sbjct: 816  KVPPHSDEPNQGFNQGRPGLLSRYMKDVHAPLLGLWGVKIVVVVIFVGLTLGSIALSTKI 875

Query: 361  EVGLEQKIVLPRDSYLQDYFDDLAEYLRIGPPLYFVVKDYNYSSKSRHTNQLCSISQCDS 420
            EVGLEQKIVLPRDSYLQDYFD+LAEYLRIGPP+YFVVKDYNYSSKSRHTNQLCSISQCDS
Sbjct: 876  EVGLEQKIVLPRDSYLQDYFDELAEYLRIGPPVYFVVKDYNYSSKSRHTNQLCSISQCDS 935

Query: 421  NSLLNEISRASLTPELNYIAKPAASWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCC 480
            NSLLNEISRASLTPEL+YIAKPAASWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCC
Sbjct: 936  NSLLNEISRASLTPELSYIAKPAASWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCC 995

Query: 481  FPDEGSCDSSEGLCKDCTTVKFYFCYIVFITIKMAFGIWYGGCFHHSDLVADRPTTVQFQ 540
            FPDEGSCDSSEG+CKDCTT                       CFHHSDL+ DRPTTVQFQ
Sbjct: 996  FPDEGSCDSSEGVCKDCTT-----------------------CFHHSDLIGDRPTTVQFQ 1055

Query: 541  EKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRSYHTPLNKQGDYVNALR 600
            EKLPWFLNSLPSADCAKGGHGAYTNSVNL GYESGIIKASEFRSYHTPLNKQGDYVNALR
Sbjct: 1056 EKLPWFLNSLPSADCAKGGHGAYTNSVNLKGYESGIIKASEFRSYHTPLNKQGDYVNALR 1115

Query: 601  AAKEFSSKISDSLKIEIFPYSVFYIFFEQYLDIWKTALMNIAIALGAIFIVSLVITSSLW 660
            AAKEFS+KISDSLK++IFPYSVFYIFFEQYLDIW+TALMNIAIALGAIFIVSLVITSSLW
Sbjct: 1116 AAKEFSAKISDSLKMDIFPYSVFYIFFEQYLDIWETALMNIAIALGAIFIVSLVITSSLW 1175

Query: 661  SSGIIILVLAMIIIDLMGVMAILKIQLNAVSVVNILMSIGIAVEFCVHLVHAFSVSCGDK 720
            SS IIILVLAMIIIDLMGVMAILKIQLNAVSVVNILMSIGIAVEFCVHLVHAF VSCGD+
Sbjct: 1176 SSAIIILVLAMIIIDLMGVMAILKIQLNAVSVVNILMSIGIAVEFCVHLVHAFLVSCGDR 1235

Query: 721  SQRAQEALSTMGASVFSGITLTKLVGVIVLCFAKTEIFVVYYFQMYLALVIIGFLHGLMF 780
            SQRAQ+ALSTMGASVFSGITLTKLVGVIVLCFAK+EIFVVYYFQMYLALVIIGFLHGL+F
Sbjct: 1236 SQRAQQALSTMGASVFSGITLTKLVGVIVLCFAKSEIFVVYYFQMYLALVIIGFLHGLVF 1295

Query: 781  LPVILSMIGPPSRYLVKDDAPMETELLVS 782
            LPVILSMIGPPSRYLVKDD PMETELLVS
Sbjct: 1296 LPVILSMIGPPSRYLVKDDDPMETELLVS 1301

BLAST of HG10000872 vs. NCBI nr
Match: XP_011656918.1 (NPC intracellular cholesterol transporter 1 isoform X1 [Cucumis sativus])

HSP 1 Score: 1361.3 bits (3522), Expect = 0.0e+00
Identity = 716/809 (88.50%), Postives = 740/809 (91.47%), Query Frame = 0

Query: 1    MDPENFDDYGGVEHAEYCFQHYASTETCFSAFKAPLDPSTSLGGFVGNNYSEASAFVVTY 60
            M+PENFDDYGGVEHAEYCFQHY S+ETCFSAFKAPLDPSTSLGGF GNNYSEASAFV+TY
Sbjct: 517  MNPENFDDYGGVEHAEYCFQHYTSSETCFSAFKAPLDPSTSLGGFFGNNYSEASAFVITY 576

Query: 61   PVNNAIDAVGNENGKAIAWEKAFVKLAKEELLPLVQSRNLTLSFSSESSIEEELKRESTA 120
            PVNNAIDAVGNENGKAIAWEKAFVKLAKEEL+PLV S+NLTLSFSSESSIEEELKRESTA
Sbjct: 577  PVNNAIDAVGNENGKAIAWEKAFVKLAKEELMPLVHSKNLTLSFSSESSIEEELKRESTA 636

Query: 121  DILTIA----------------------------VLLGLSGVILVVLSVLGSVGFFSAIG 180
            DILTIA                            VLLGLSGVILVVLSVLGS+GFFSAIG
Sbjct: 637  DILTIAVSYLVMFAYISVALGDSNISSSFYLSSKVLLGLSGVILVVLSVLGSIGFFSAIG 696

Query: 181  VKSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQPYELTLEERISSALVEVGPSITLASLS 240
            +KSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQPYEL+LEERIS ALVEVGPSITLASLS
Sbjct: 697  IKSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQPYELSLEERISCALVEVGPSITLASLS 756

Query: 241  EILAFAVGTFVPMPACRVFSMFAALAVLLDFILQLSAFVALIVFDILRAEDHRVDCFPCI 300
            EILAFAVGTFVPMPACRVFSMFAALAVLLDFILQLSAFVALIV DILRAEDHRVDCFPCI
Sbjct: 757  EILAFAVGTFVPMPACRVFSMFAALAVLLDFILQLSAFVALIVLDILRAEDHRVDCFPCI 816

Query: 301  KVPPHSDELNQGFNQGRPGLLSRYMKDVHAPLLGLWGVKIVVVTIFVGLTLGSIALSTKI 360
            KV PHSDE NQGFNQGR GLLSRYMKDVHAP LG WGVKIVVV IFVGLTLGSIALSTKI
Sbjct: 817  KVHPHSDEPNQGFNQGRHGLLSRYMKDVHAPFLGFWGVKIVVVVIFVGLTLGSIALSTKI 876

Query: 361  EVGLEQKIVLPRDSYLQDYFDDLAEYLRIGPPLYFVVKDYNYSSKSRHTNQLCSISQCDS 420
            EVGLEQKIVLPRDSYLQDYFDDLAEYLRIGPPLYFVVKDYNYSSKS+ TNQLCSIS CDS
Sbjct: 877  EVGLEQKIVLPRDSYLQDYFDDLAEYLRIGPPLYFVVKDYNYSSKSKETNQLCSISHCDS 936

Query: 421  NSLLNEISRASLTPELNYIAKPAASWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCC 480
            NSLLNEI+RASLTPELNYIAKPAASWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCC
Sbjct: 937  NSLLNEIARASLTPELNYIAKPAASWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCC 996

Query: 481  FPDEGSCDSSEGLCKDCTTVKFYFCYIVFITIKMAFGIWYGGCFHHSDLVADRPTTVQFQ 540
            FPD+G CDSSEG+CKDCTT                       CFHHSDLV  RPTTVQFQ
Sbjct: 997  FPDDGFCDSSEGVCKDCTT-----------------------CFHHSDLVGGRPTTVQFQ 1056

Query: 541  EKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRSYHTPLNKQGDYVNALR 600
            EKLPWFLNSLPSADCAKGGHGAYTNSVNL GYESGIIKASEFRSYHTPLNKQGDYVNALR
Sbjct: 1057 EKLPWFLNSLPSADCAKGGHGAYTNSVNLKGYESGIIKASEFRSYHTPLNKQGDYVNALR 1116

Query: 601  AAKEFSSKISDSLKIEIFPYSVFYIFFEQYLDIWKTALMNIAIALGAIFIVSLVITSSLW 660
            AAK+FSSKISDSLK++IFPYSVFYIFFEQYLDIWKTALMNIAIALGAIFIVSLVITSSLW
Sbjct: 1117 AAKDFSSKISDSLKMDIFPYSVFYIFFEQYLDIWKTALMNIAIALGAIFIVSLVITSSLW 1176

Query: 661  SSGIIILVLAMIIIDLMGVMAILKIQLNAVSVVNILMSIGIAVEFCVHLVHAFSVSCGDK 720
            SSG+IILVLAMI+IDL+GVMA+LKIQLNAVSVVNILMSIGIAVEFCVHLVHAFSVSCGD+
Sbjct: 1177 SSGMIILVLAMIVIDLLGVMAVLKIQLNAVSVVNILMSIGIAVEFCVHLVHAFSVSCGDR 1236

Query: 721  SQRAQEALSTMGASVFSGITLTKLVGVIVLCFAKTEIFVVYYFQMYLALVIIGFLHGLMF 780
            SQRAQEALST+GASVFSGITLTKLVGVIVLCFAK+EIFVVYYFQMYLALVIIGFLHGL+F
Sbjct: 1237 SQRAQEALSTIGASVFSGITLTKLVGVIVLCFAKSEIFVVYYFQMYLALVIIGFLHGLVF 1296

Query: 781  LPVILSMIGPPSRYLVKDDAPMETELLVS 782
            LPVILSMIGPPSRYL+ DDAPMETELLVS
Sbjct: 1297 LPVILSMIGPPSRYLISDDAPMETELLVS 1302

BLAST of HG10000872 vs. NCBI nr
Match: KAE8646830.1 (hypothetical protein Csa_018310 [Cucumis sativus])

HSP 1 Score: 1361.3 bits (3522), Expect = 0.0e+00
Identity = 716/809 (88.50%), Postives = 740/809 (91.47%), Query Frame = 0

Query: 1    MDPENFDDYGGVEHAEYCFQHYASTETCFSAFKAPLDPSTSLGGFVGNNYSEASAFVVTY 60
            M+PENFDDYGGVEHAEYCFQHY S+ETCFSAFKAPLDPSTSLGGF GNNYSEASAFV+TY
Sbjct: 278  MNPENFDDYGGVEHAEYCFQHYTSSETCFSAFKAPLDPSTSLGGFFGNNYSEASAFVITY 337

Query: 61   PVNNAIDAVGNENGKAIAWEKAFVKLAKEELLPLVQSRNLTLSFSSESSIEEELKRESTA 120
            PVNNAIDAVGNENGKAIAWEKAFVKLAKEEL+PLV S+NLTLSFSSESSIEEELKRESTA
Sbjct: 338  PVNNAIDAVGNENGKAIAWEKAFVKLAKEELMPLVHSKNLTLSFSSESSIEEELKRESTA 397

Query: 121  DILTIA----------------------------VLLGLSGVILVVLSVLGSVGFFSAIG 180
            DILTIA                            VLLGLSGVILVVLSVLGS+GFFSAIG
Sbjct: 398  DILTIAVSYLVMFAYISVALGDSNISSSFYLSSKVLLGLSGVILVVLSVLGSIGFFSAIG 457

Query: 181  VKSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQPYELTLEERISSALVEVGPSITLASLS 240
            +KSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQPYEL+LEERIS ALVEVGPSITLASLS
Sbjct: 458  IKSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQPYELSLEERISCALVEVGPSITLASLS 517

Query: 241  EILAFAVGTFVPMPACRVFSMFAALAVLLDFILQLSAFVALIVFDILRAEDHRVDCFPCI 300
            EILAFAVGTFVPMPACRVFSMFAALAVLLDFILQLSAFVALIV DILRAEDHRVDCFPCI
Sbjct: 518  EILAFAVGTFVPMPACRVFSMFAALAVLLDFILQLSAFVALIVLDILRAEDHRVDCFPCI 577

Query: 301  KVPPHSDELNQGFNQGRPGLLSRYMKDVHAPLLGLWGVKIVVVTIFVGLTLGSIALSTKI 360
            KV PHSDE NQGFNQGR GLLSRYMKDVHAP LG WGVKIVVV IFVGLTLGSIALSTKI
Sbjct: 578  KVHPHSDEPNQGFNQGRHGLLSRYMKDVHAPFLGFWGVKIVVVVIFVGLTLGSIALSTKI 637

Query: 361  EVGLEQKIVLPRDSYLQDYFDDLAEYLRIGPPLYFVVKDYNYSSKSRHTNQLCSISQCDS 420
            EVGLEQKIVLPRDSYLQDYFDDLAEYLRIGPPLYFVVKDYNYSSKS+ TNQLCSIS CDS
Sbjct: 638  EVGLEQKIVLPRDSYLQDYFDDLAEYLRIGPPLYFVVKDYNYSSKSKETNQLCSISHCDS 697

Query: 421  NSLLNEISRASLTPELNYIAKPAASWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCC 480
            NSLLNEI+RASLTPELNYIAKPAASWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCC
Sbjct: 698  NSLLNEIARASLTPELNYIAKPAASWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCC 757

Query: 481  FPDEGSCDSSEGLCKDCTTVKFYFCYIVFITIKMAFGIWYGGCFHHSDLVADRPTTVQFQ 540
            FPD+G CDSSEG+CKDCTT                       CFHHSDLV  RPTTVQFQ
Sbjct: 758  FPDDGFCDSSEGVCKDCTT-----------------------CFHHSDLVGGRPTTVQFQ 817

Query: 541  EKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRSYHTPLNKQGDYVNALR 600
            EKLPWFLNSLPSADCAKGGHGAYTNSVNL GYESGIIKASEFRSYHTPLNKQGDYVNALR
Sbjct: 818  EKLPWFLNSLPSADCAKGGHGAYTNSVNLKGYESGIIKASEFRSYHTPLNKQGDYVNALR 877

Query: 601  AAKEFSSKISDSLKIEIFPYSVFYIFFEQYLDIWKTALMNIAIALGAIFIVSLVITSSLW 660
            AAK+FSSKISDSLK++IFPYSVFYIFFEQYLDIWKTALMNIAIALGAIFIVSLVITSSLW
Sbjct: 878  AAKDFSSKISDSLKMDIFPYSVFYIFFEQYLDIWKTALMNIAIALGAIFIVSLVITSSLW 937

Query: 661  SSGIIILVLAMIIIDLMGVMAILKIQLNAVSVVNILMSIGIAVEFCVHLVHAFSVSCGDK 720
            SSG+IILVLAMI+IDL+GVMA+LKIQLNAVSVVNILMSIGIAVEFCVHLVHAFSVSCGD+
Sbjct: 938  SSGMIILVLAMIVIDLLGVMAVLKIQLNAVSVVNILMSIGIAVEFCVHLVHAFSVSCGDR 997

Query: 721  SQRAQEALSTMGASVFSGITLTKLVGVIVLCFAKTEIFVVYYFQMYLALVIIGFLHGLMF 780
            SQRAQEALST+GASVFSGITLTKLVGVIVLCFAK+EIFVVYYFQMYLALVIIGFLHGL+F
Sbjct: 998  SQRAQEALSTIGASVFSGITLTKLVGVIVLCFAKSEIFVVYYFQMYLALVIIGFLHGLVF 1057

Query: 781  LPVILSMIGPPSRYLVKDDAPMETELLVS 782
            LPVILSMIGPPSRYL+ DDAPMETELLVS
Sbjct: 1058 LPVILSMIGPPSRYLISDDAPMETELLVS 1063

BLAST of HG10000872 vs. NCBI nr
Match: KAA0047847.1 (Niemann-Pick C1 protein-like isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 1350.9 bits (3495), Expect = 0.0e+00
Identity = 712/809 (88.01%), Postives = 738/809 (91.22%), Query Frame = 0

Query: 1    MDPENFDDYGGVEHAEYCFQHYASTETCFSAFKAPLDPSTSLGGFVGNNYSEASAFVVTY 60
            M+PENFDDYGGVEHAEYCFQHY S+ETCFSAFKAPLDPSTSLGGF G+NYSEASAFV+TY
Sbjct: 497  MNPENFDDYGGVEHAEYCFQHYTSSETCFSAFKAPLDPSTSLGGFFGSNYSEASAFVITY 556

Query: 61   PVNNAIDAVGNENGKAIAWEKAFVKLAKEELLPLVQSRNLTLSFSSESSIEEELKRESTA 120
            PVNNAIDAVGNENGKAIAWEKAFVKLAKEEL+PLV SRNLTLSFSSESSIEEELKRESTA
Sbjct: 557  PVNNAIDAVGNENGKAIAWEKAFVKLAKEELMPLVHSRNLTLSFSSESSIEEELKRESTA 616

Query: 121  DILTIA----------------------------VLLGLSGVILVVLSVLGSVGFFSAIG 180
            DILTIA                            VLLGLSGVILVVLSVLGSVGFFSAIG
Sbjct: 617  DILTIAVSYLVMFAYISVALGDSNISSSFYLSSKVLLGLSGVILVVLSVLGSVGFFSAIG 676

Query: 181  VKSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQPYELTLEERISSALVEVGPSITLASLS 240
            +KSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQPYEL+LE+RISSALVEVGPSITLASLS
Sbjct: 677  IKSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQPYELSLEDRISSALVEVGPSITLASLS 736

Query: 241  EILAFAVGTFVPMPACRVFSMFAALAVLLDFILQLSAFVALIVFDILRAEDHRVDCFPCI 300
            EILAFAVGTFVPMPACRVFS+FAALAVLLDFILQLSAFVALIV DILRAEDHRVDCFPCI
Sbjct: 737  EILAFAVGTFVPMPACRVFSLFAALAVLLDFILQLSAFVALIVLDILRAEDHRVDCFPCI 796

Query: 301  KVPPHSDELNQGFNQGRPGLLSRYMKDVHAPLLGLWGVKIVVVTIFVGLTLGSIALSTKI 360
            KV PHSDE NQGFNQGR GLLSRYMKDVHAPLLG WGVKIVVV IFVGLTLGSIALSTKI
Sbjct: 797  KVHPHSDEPNQGFNQGRHGLLSRYMKDVHAPLLGFWGVKIVVVVIFVGLTLGSIALSTKI 856

Query: 361  EVGLEQKIVLPRDSYLQDYFDDLAEYLRIGPPLYFVVKDYNYSSKSRHTNQLCSISQCDS 420
            EVGLEQKIVLPRDSYLQDYFDDLAEYLRIGPPLYFVVKDYNYSS+SR TNQLCSIS CDS
Sbjct: 857  EVGLEQKIVLPRDSYLQDYFDDLAEYLRIGPPLYFVVKDYNYSSRSRQTNQLCSISHCDS 916

Query: 421  NSLLNEISRASLTPELNYIAKPAASWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCC 480
            NSLLNEISRASLTPELNYIAKPAASWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCC
Sbjct: 917  NSLLNEISRASLTPELNYIAKPAASWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCC 976

Query: 481  FPDEGSCDSSEGLCKDCTTVKFYFCYIVFITIKMAFGIWYGGCFHHSDLVADRPTTVQFQ 540
            FPDEG CDSSEG+C+DCTT                       CF HSDLV DRPTT QF+
Sbjct: 977  FPDEGFCDSSEGVCRDCTT-----------------------CFRHSDLVGDRPTTGQFK 1036

Query: 541  EKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRSYHTPLNKQGDYVNALR 600
            EKLPWFLNSLPSADCAKGGHGAYTNSVNL GYE GIIKASEFRSYHTPLNKQGDYVNALR
Sbjct: 1037 EKLPWFLNSLPSADCAKGGHGAYTNSVNLKGYEIGIIKASEFRSYHTPLNKQGDYVNALR 1096

Query: 601  AAKEFSSKISDSLKIEIFPYSVFYIFFEQYLDIWKTALMNIAIALGAIFIVSLVITSSLW 660
            AAKEF SKISDSLK++IFPYSVFYIFFEQYLDIW TALMNIAIALGAIFIVSLVITSSLW
Sbjct: 1097 AAKEFCSKISDSLKMDIFPYSVFYIFFEQYLDIWNTALMNIAIALGAIFIVSLVITSSLW 1156

Query: 661  SSGIIILVLAMIIIDLMGVMAILKIQLNAVSVVNILMSIGIAVEFCVHLVHAFSVSCGDK 720
             SG+IILVLAMI+IDL+GVMAILKIQLNAVSVVNILMSIGIAVEFCVHLVHAFSVSCGD+
Sbjct: 1157 CSGMIILVLAMIVIDLLGVMAILKIQLNAVSVVNILMSIGIAVEFCVHLVHAFSVSCGDR 1216

Query: 721  SQRAQEALSTMGASVFSGITLTKLVGVIVLCFAKTEIFVVYYFQMYLALVIIGFLHGLMF 780
            +QRAQEALST+GASVFSGITLTKLVGV+VLCFAK+EIFVVYYFQMYLALVIIGFLHGL+F
Sbjct: 1217 NQRAQEALSTIGASVFSGITLTKLVGVVVLCFAKSEIFVVYYFQMYLALVIIGFLHGLVF 1276

Query: 781  LPVILSMIGPPSRYLVKDDAPMETELLVS 782
            LPVILSMIGPPSRYL+KDDAP+ETELLVS
Sbjct: 1277 LPVILSMIGPPSRYLIKDDAPVETELLVS 1282

BLAST of HG10000872 vs. NCBI nr
Match: XP_022140528.1 (Niemann-Pick C1 protein [Momordica charantia])

HSP 1 Score: 1349.3 bits (3491), Expect = 0.0e+00
Identity = 708/809 (87.52%), Postives = 737/809 (91.10%), Query Frame = 0

Query: 1    MDPENFDDYGGVEHAEYCFQHYASTETCFSAFKAPLDPSTSLGGFVGNNYSEASAFVVTY 60
            MDPEN+DDYGGV+HAEYCFQHYASTETCFSAFKAPLDPSTSLGGF GNNYSEASAFVVTY
Sbjct: 515  MDPENYDDYGGVQHAEYCFQHYASTETCFSAFKAPLDPSTSLGGFSGNNYSEASAFVVTY 574

Query: 61   PVNNAIDAVGNENGKAIAWEKAFVKLAKEELLPLVQSRNLTLSFSSESSIEEELKRESTA 120
            PVNNAIDAVGNENGKA+AWEKAFVKL KEELLPLVQSRNLTLSFSSESSIEEELKRESTA
Sbjct: 575  PVNNAIDAVGNENGKAVAWEKAFVKLVKEELLPLVQSRNLTLSFSSESSIEEELKRESTA 634

Query: 121  DILTIA----------------------------VLLGLSGVILVVLSVLGSVGFFSAIG 180
            D+LTI                             VLLGLSGVILVVLSVLGSVGFFSAIG
Sbjct: 635  DVLTIVVSYLVMFVYISVALGDSSISSSFYLSSKVLLGLSGVILVVLSVLGSVGFFSAIG 694

Query: 181  VKSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQPYELTLEERISSALVEVGPSITLASLS 240
            VKSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQP+ELTLEERISSALVEVGPSITLASLS
Sbjct: 695  VKSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQPFELTLEERISSALVEVGPSITLASLS 754

Query: 241  EILAFAVGTFVPMPACRVFSMFAALAVLLDFILQLSAFVALIVFDILRAEDHRVDCFPCI 300
            EILAFAVG FVPMPACRVFSMFAALAVLLDFILQLSAFVALIVFDILRAEDHRVDCFPCI
Sbjct: 755  EILAFAVGAFVPMPACRVFSMFAALAVLLDFILQLSAFVALIVFDILRAEDHRVDCFPCI 814

Query: 301  KVPPHSDELNQGFNQGRPGLLSRYMKDVHAPLLGLWGVKIVVVTIFVGLTLGSIALSTKI 360
            KV PHSDE NQGFNQG+PGLLS YMKDVHAPLLGLWGVKI V+ IFVG TLG IALSTKI
Sbjct: 815  KVTPHSDEPNQGFNQGKPGLLSWYMKDVHAPLLGLWGVKIAVIVIFVGFTLGGIALSTKI 874

Query: 361  EVGLEQKIVLPRDSYLQDYFDDLAEYLRIGPPLYFVVKDYNYSSKSRHTNQLCSISQCDS 420
            EVGLEQKIVLPRDSYLQDYFDDLAEYLRIGPPLYFVVKDYNYSS+SR TNQLCSISQCDS
Sbjct: 875  EVGLEQKIVLPRDSYLQDYFDDLAEYLRIGPPLYFVVKDYNYSSESRQTNQLCSISQCDS 934

Query: 421  NSLLNEISRASLTPELNYIAKPAASWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCC 480
            NSLLNEISRASLTPELNYIAKPAASWLDDFLVWLSP+AFGCCRKFTNGSYCPPDDQPPCC
Sbjct: 935  NSLLNEISRASLTPELNYIAKPAASWLDDFLVWLSPDAFGCCRKFTNGSYCPPDDQPPCC 994

Query: 481  FPDEGSCDSSEGLCKDCTTVKFYFCYIVFITIKMAFGIWYGGCFHHSDLVADRPTTVQFQ 540
            FPDEGSCDSS G+CKDCTT                       CFH+SDLVA RPTT+QFQ
Sbjct: 995  FPDEGSCDSSGGVCKDCTT-----------------------CFHYSDLVAGRPTTIQFQ 1054

Query: 541  EKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRSYHTPLNKQGDYVNALR 600
            EKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFR+YHTPLNKQGDYVNALR
Sbjct: 1055 EKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRTYHTPLNKQGDYVNALR 1114

Query: 601  AAKEFSSKISDSLKIEIFPYSVFYIFFEQYLDIWKTALMNIAIALGAIFIVSLVITSSLW 660
            AAKEFSSK+SDSLK++IFPYSVFYIFFEQYLDIWK AL+NIA+ALGA+F+VSLVITS LW
Sbjct: 1115 AAKEFSSKMSDSLKMDIFPYSVFYIFFEQYLDIWKIALINIAVALGAVFVVSLVITSCLW 1174

Query: 661  SSGIIILVLAMIIIDLMGVMAILKIQLNAVSVVNILMSIGIAVEFCVHLVHAFSVSCGDK 720
            SSGIIILVLAMIIIDLMG+MAIL IQLNAVSVVN+LMSIGIAVEFCVH+VHAF VS GD+
Sbjct: 1175 SSGIIILVLAMIIIDLMGIMAILNIQLNAVSVVNLLMSIGIAVEFCVHIVHAFLVSSGDR 1234

Query: 721  SQRAQEALSTMGASVFSGITLTKLVGVIVLCFAKTEIFVVYYFQMYLALVIIGFLHGLMF 780
            SQRA+EAL TMGASVFSGITLTKLVGVIVLCF+++EIFVVYYFQMYLALVIIGFLHGL+F
Sbjct: 1235 SQRAREALGTMGASVFSGITLTKLVGVIVLCFSRSEIFVVYYFQMYLALVIIGFLHGLVF 1294

Query: 781  LPVILSMIGPPSRYLVKDDAPMETELLVS 782
            LPVILSMIGPPSRYLVKDDAP ETELLVS
Sbjct: 1295 LPVILSMIGPPSRYLVKDDAPTETELLVS 1300

BLAST of HG10000872 vs. ExPASy Swiss-Prot
Match: O35604 (NPC intracellular cholesterol transporter 1 OS=Mus musculus OX=10090 GN=Npc1 PE=1 SV=2)

HSP 1 Score: 479.9 bits (1234), Expect = 5.3e-134
Identity = 308/795 (38.74%), Postives = 463/795 (58.24%), Query Frame = 0

Query: 14   HAEYCFQHYASTE-------TCFSAFKAPLDPSTSLGGFVGNNYSEASAFVVTYPVNNAI 73
            H  YC +  AS          C   F  P+ P   LGG+   NY+ A+A V+T+PVNN  
Sbjct: 512  HFLYCVRAPASLNDTSLLHGPCLGTFGGPVFPWLVLGGYDDQNYNNATALVITFPVNNYY 571

Query: 74   DAVGNENGKAIAWEKAFVKLAKEELLPLVQSRNLTLSFSSESSIEEELKRESTADILTI- 133
            +       +A AWEK F+   K    P     NLT+SF++E SIE+EL RES +D+ T+ 
Sbjct: 572  NDT-ERLQRAWAWEKEFISFVKNYKNP-----NLTISFTAERSIEDELNRESNSDVFTVI 631

Query: 134  ---------------------------AVLLGLSGVILVVLSVLGSVGFFSAIGVKSTLI 193
                                        + LG++G+++V+ SV  S+G FS +G+  TLI
Sbjct: 632  ISYVVMFLYISLALGHIQSCSRLLVDSKISLGIAGILIVLSSVACSLGIFSYMGMPLTLI 691

Query: 194  IMEVIPFLVLAVGVDNMCILVHAVKRQP--YELTLEERISSALVEVGPSITLASLSEILA 253
            ++EVIPFLVLAVGVDN+ ILV   +R     E TL++++   L EV P++ L+S SE  A
Sbjct: 692  VIEVIPFLVLAVGVDNIFILVQTYQRDERLQEETLDQQLGRILGEVAPTMFLSSFSETSA 751

Query: 254  FAVGTFVPMPACRVFSMFAALAVLLDFILQLSAFVALIVFDILRAEDHRVDCFPCIKVPP 313
            F  G    MPA   FS+FA +AVL+DF+LQ++ FV+L+  DI R E + +D   C++   
Sbjct: 752  FFFGALSSMPAVHTFSLFAGMAVLIDFLLQITCFVSLLGLDIKRQEKNHLDILCCVR--- 811

Query: 314  HSDELNQGFNQGRPGLLSRYMKDVHAPLLGLWGVKIVVVTIFVGLTLGSIALSTKIEVGL 373
               +  QG +      L R+ K+  APLL    ++ +VV +FVG+   S+A+  K+++GL
Sbjct: 812  -GADDGQG-SHASESYLFRFFKNYFAPLLLKDWLRPIVVAVFVGVLSFSVAVVNKVDIGL 871

Query: 374  EQKIVLPRDSYLQDYFDDLAEYLRIGPPLYFVVKD-YNYSSKSRHTNQLCSISQCDSNSL 433
            +Q + +P DSY+ DYF  LA+YL  GPP+YFV+++ YNYSS+ +  N +C    CD++SL
Sbjct: 872  DQSLSMPNDSYVIDYFKSLAQYLHSGPPVYFVLEEGYNYSSR-KGQNMVCGGMGCDNDSL 931

Query: 434  LNEISRASLTPELNYIAKPAASWLDDFLVWLSPEAFGCCRKF-TNGSYCPPDDQPPCCFP 493
            + +I  A+       +    +SW+DD+  W+SP++  CCR +     +C      P C  
Sbjct: 932  VQQIFNAAELDTYTRVGFAPSSWIDDYFDWVSPQS-SCCRLYNVTHQFCNASVMDPTCV- 991

Query: 494  DEGSCDSSEGLCKDCTTVKFYFCYIVFITIKMAFGIWYGGCFHHSDLVADRPTTVQFQEK 553
                       C+  T                                  RP   +F + 
Sbjct: 992  ----------RCRPLTPEG-----------------------------KQRPQGKEFMKF 1051

Query: 554  LPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRSYHTPLNKQGDYVNALRAA 613
            LP FL+  P+  C KGGH AY ++VN+ G +   I A+ F +YHT L    DY +A++ A
Sbjct: 1052 LPMFLSDNPNPKCGKGGHAAYGSAVNIVG-DDTYIGATYFMTYHTILKTSADYTDAMKKA 1111

Query: 614  KEFSSKISDSLK-----IEIFPYSVFYIFFEQYLDIWKTALMNIAIALGAIFIVSLVITS 673
            +  +S I+++++       +FPYSVFY+F+EQYL I    + N++++LG+IF+V+LV+  
Sbjct: 1112 RLIASNITETMRSKGSDYRVFPYSVFYVFYEQYLTIIDDTIFNLSVSLGSIFLVTLVVLG 1171

Query: 674  -SLWSSGIIILVLAMIIIDLMGVMAILKIQLNAVSVVNILMSIGIAVEFCVHLVHAFSVS 733
              LWS+ I+ + +AMI++++ GVM +  I LNAVS+VN++MS GI+VEFC H+  AF++S
Sbjct: 1172 CELWSAVIMCITIAMILVNMFGVMWLWGISLNAVSLVNLVMSCGISVEFCSHITRAFTMS 1231

Query: 734  C-GDKSQRAQEALSTMGASVFSGITLTKLVGVIVLCFAKTEIFVVYYFQMYLALVIIGFL 763
              G +  RA+EAL+ MG+SVFSGITLTK  G++VL FAK++IF ++YF+MYLA+V++G  
Sbjct: 1232 TKGSRVSRAEEALAHMGSSVFSGITLTKFGGIVVLAFAKSQIFEIFYFRMYLAMVLLGAT 1252

BLAST of HG10000872 vs. ExPASy Swiss-Prot
Match: O15118 (NPC intracellular cholesterol transporter 1 OS=Homo sapiens OX=9606 GN=NPC1 PE=1 SV=2)

HSP 1 Score: 477.2 bits (1227), Expect = 3.4e-133
Identity = 303/794 (38.16%), Postives = 459/794 (57.81%), Query Frame = 0

Query: 14   HAEYCFQHYAST-------ETCFSAFKAPLDPSTSLGGFVGNNYSEASAFVVTYPVNNAI 73
            H  YC +  AS        + C   F  P+ P   LGG+   NY+ A+A V+T+PVNN  
Sbjct: 512  HFLYCVRAPASLNDTSLLHDPCLGTFGGPVFPWLVLGGYDDQNYNNATALVITFPVNNYY 571

Query: 74   DAVGNENGKAIAWEKAFVKLAKEELLPLVQSRNLTLSFSSESSIEEELKRESTADILTIA 133
            +    +  +A AWEK F+   K    P     NLT+SF++E SIE+EL RES +D+ T+ 
Sbjct: 572  NDT-EKLQRAQAWEKEFINFVKNYKNP-----NLTISFTAERSIEDELNRESDSDVFTVV 631

Query: 134  ----------------------------VLLGLSGVILVVLSVLGSVGFFSAIGVKSTLI 193
                                        V LG++G+++V+ SV  S+G FS IG+  TLI
Sbjct: 632  ISYAIMFLYISLALGHMKSCRRLLVDSKVSLGIAGILIVLSSVACSLGVFSYIGLPLTLI 691

Query: 194  IMEVIPFLVLAVGVDNMCILVHAVKRQP--YELTLEERISSALVEVGPSITLASLSEILA 253
            ++EVIPFLVLAVGVDN+ ILV A +R       TL++++   L EV PS+ L+S SE +A
Sbjct: 692  VIEVIPFLVLAVGVDNIFILVQAYQRDERLQGETLDQQLGRVLGEVAPSMFLSSFSETVA 751

Query: 254  FAVGTFVPMPACRVFSMFAALAVLLDFILQLSAFVALIVFDILRAEDHRVDCFPCIKVPP 313
            F +G    MPA   FS+FA LAV +DF+LQ++ FV+L+  DI R E +R+D F C++   
Sbjct: 752  FFLGALSVMPAVHTFSLFAGLAVFIDFLLQITCFVSLLGLDIKRQEKNRLDIFCCVRGAE 811

Query: 314  HSDELNQGFNQGRPGLLSRYMKDVHAPLLGLWGVKIVVVTIFVGLTLGSIALSTKIEVGL 373
                +     Q     L R+ K+ ++PLL    ++ +V+ IFVG+   SIA+  K+++GL
Sbjct: 812  DGTSV-----QASESCLFRFFKNSYSPLLLKDWMRPIVIAIFVGVLSFSIAVLNKVDIGL 871

Query: 374  EQKIVLPRDSYLQDYFDDLAEYLRIGPPLYFVVKDYNYSSKSRHTNQLCSISQCDSNSLL 433
            +Q + +P DSY+ DYF  +++YL  GPP+YFV+++ +  + S+  N +C    C+++SL+
Sbjct: 872  DQSLSMPDDSYMVDYFKSISQYLHAGPPVYFVLEEGHDYTSSKGQNMVCGGMGCNNDSLV 931

Query: 434  NEISRASLTPELNYIAKPAASWLDDFLVWLSPEAFGCCR-KFTNGSYCPPDDQPPCCFPD 493
             +I  A+       I    +SW+DD+  W+ P++  CCR       +C      P C   
Sbjct: 932  QQIFNAAQLDNYTRIGFAPSSWIDDYFDWVKPQS-SCCRVDNITDQFCNASVVDPACV-- 991

Query: 494  EGSCDSSEGLCKDCTTVKFYFCYIVFITIKMAFGIWYGGCFHHSDLVADRPTTVQFQEKL 553
                      C+  T                                  RP    F   L
Sbjct: 992  ---------RCRPLTPEG-----------------------------KQRPQGGDFMRFL 1051

Query: 554  PWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRSYHTPLNKQGDYVNALRAAK 613
            P FL+  P+  C KGGH AY+++VN+       + A+ F +YHT L    D+++AL+ A+
Sbjct: 1052 PMFLSDNPNPKCGKGGHAAYSSAVNILLGHGTRVGATYFMTYHTVLQTSADFIDALKKAR 1111

Query: 614  EFSSKISDSLKI-----EIFPYSVFYIFFEQYLDIWKTALMNIAIALGAIFIVSLVITS- 673
              +S +++++ I      +FPYSVFY+F+EQYL I    + N+ ++LGAIF+V++V+   
Sbjct: 1112 LIASNVTETMGINGSAYRVFPYSVFYVFYEQYLTIIDDTIFNLGVSLGAIFLVTMVLLGC 1171

Query: 674  SLWSSGIIILVLAMIIIDLMGVMAILKIQLNAVSVVNILMSIGIAVEFCVHLVHAFSVSC 733
             LWS+ I+   +AM+++++ GVM +  I LNAVS+VN++MS GI+VEFC H+  AF+VS 
Sbjct: 1172 ELWSAVIMCATIAMVLVNMFGVMWLWGISLNAVSLVNLVMSCGISVEFCSHITRAFTVSM 1231

Query: 734  -GDKSQRAQEALSTMGASVFSGITLTKLVGVIVLCFAKTEIFVVYYFQMYLALVIIGFLH 763
             G + +RA+EAL+ MG+SVFSGITLTK  G++VL FAK++IF ++YF+MYLA+V++G  H
Sbjct: 1232 KGSRVERAEEALAHMGSSVFSGITLTKFGGIVVLAFAKSQIFQIFYFRMYLAMVLLGATH 1253

BLAST of HG10000872 vs. ExPASy Swiss-Prot
Match: P56941 (NPC intracellular cholesterol transporter 1 OS=Sus scrofa OX=9823 GN=NPC1 PE=2 SV=1)

HSP 1 Score: 474.9 bits (1221), Expect = 1.7e-132
Identity = 308/795 (38.74%), Postives = 464/795 (58.36%), Query Frame = 0

Query: 14   HAEYCFQHYAST-------ETCFSAFKAPLDPSTSLGGFVGNNYSEASAFVVTYPVNNAI 73
            H  YC +  AS        + C   F  P+ P   LGG+   NY+ A+A V+T+PVNN  
Sbjct: 512  HFLYCVRAPASLNDASLLHDPCLGTFGGPVFPWLVLGGYDDQNYNNATALVITFPVNNYY 571

Query: 74   DAVGNENGKAIAWEKAFVKLAKEELLPLVQSRNLTLSFSSESSIEEELKRESTADILTI- 133
            +    +  +A AWE  F+   K    P     NLT+SF +E SIE+EL RES +D+ TI 
Sbjct: 572  NDT-EKLQRAQAWESEFINFVKNYKNP-----NLTISFMAERSIEDELNRESNSDLFTIL 631

Query: 134  ---------------------------AVLLGLSGVILVVLSVLGSVGFFSAIGVKSTLI 193
                                        + LG++G+++V+ SV  S+G FS IGV  TLI
Sbjct: 632  ISYAIMFLYISIALGHIKSCSRLLVDSKISLGIAGILIVLSSVACSLGIFSYIGVPLTLI 691

Query: 194  IMEVIPFLVLAVGVDNMCILVHAVKRQP--YELTLEERISSALVEVGPSITLASLSEILA 253
            ++EVIPFLVLAVGVDN+ ILV   +R       TL++++   L EV PS+ L+S SE +A
Sbjct: 692  VIEVIPFLVLAVGVDNIFILVQTYQRDERLQGETLDQQLGRVLGEVAPSMFLSSFSETVA 751

Query: 254  FAVGTFVPMPACRVFSMFAALAVLLDFILQLSAFVALIVFDILRAEDHRVDCFPCIKVPP 313
            F +G    +PA   FS+FA +AVL+DF+LQ++ FV+L+  DI R E +R+D   C++   
Sbjct: 752  FFLGGLSVVPAVHTFSLFAGMAVLIDFLLQITCFVSLLGLDIKRQEKNRLDVVCCVQ--- 811

Query: 314  HSDELNQGFNQGRPGLLSRYMKDVHAPLLGLWGVKIVVVTIFVGLTLGSIALSTKIEVGL 373
               E   G  Q     L R+ K+ +APLL    ++ +V+ +FVG+   SIA+  K+E+GL
Sbjct: 812  -GAEDGAGV-QASESCLFRFFKNSYAPLLLKDWMRPIVIAVFVGVLSFSIAVLNKVEIGL 871

Query: 374  EQKIVLPRDSYLQDYFDDLAEYLRIGPPLYFVVKD-YNYSSKSRHTNQLCSISQCDSNSL 433
            +Q + +P DSY+ DYF  L+ YL  GPP+YFVV++ +NY+S  +  N +C    C+++SL
Sbjct: 872  DQSLSMPDDSYVMDYFQSLSRYLHAGPPVYFVVEEGHNYTS-LKGQNMVCGGLGCNNDSL 931

Query: 434  LNEISRASLTPELNYIAKPAASWLDDFLVWLSPEAFGCCRKF-TNGSYCPPDDQPPCCFP 493
            + +I  A+       I    +SW+DD+  W+ P++  CCR + +   +C      P C  
Sbjct: 932  VQQIFTAAQLDNYTRIGFAPSSWIDDYFDWIKPQS-SCCRVYNSTDQFCNASVVDPTCI- 991

Query: 494  DEGSCDSSEGLCKDCTTVKFYFCYIVFITIKMAFGIWYGGCFHHSDLVADRPTTVQFQEK 553
                       C+  T+                                 RP    F   
Sbjct: 992  ----------RCRPLTSEG-----------------------------KQRPQGEDFMRF 1051

Query: 554  LPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRSYHTPLNKQGDYVNALRAA 613
            LP FL+  P+  C KGGH AY+++VN+ G  SG + A+ F +YHT L    D+++A++ A
Sbjct: 1052 LPMFLSDNPNPKCGKGGHAAYSSAVNILGNGSG-VGATYFMTYHTVLQASADFIDAMQKA 1111

Query: 614  KEFSSKISDSLKIE-----IFPYSVFYIFFEQYLDIWKTALMNIAIALGAIFIVSLVITS 673
            +  +S I+ ++ +E     +FPYSVFY+F+EQYL +    + N+ ++LGAIF+V++V+  
Sbjct: 1112 RLIASNITRTMGLEASSYRVFPYSVFYVFYEQYLTVIDDTIFNLGVSLGAIFLVTVVLMG 1171

Query: 674  -SLWSSGIIILVLAMIIIDLMGVMAILKIQLNAVSVVNILMSIGIAVEFCVHLVHAFSVS 733
              LW++ I+ + +AMI++++ GVM +  I LNAVS+VN++MS GI+VEFC H+  AF++S
Sbjct: 1172 CELWATVIMCVTIAMILVNMFGVMWLWGISLNAVSLVNLVMSCGISVEFCSHITRAFTLS 1231

Query: 734  C-GDKSQRAQEALSTMGASVFSGITLTKLVGVIVLCFAKTEIFVVYYFQMYLALVIIGFL 763
              G +  RA+EAL+ MG+SVFSGITLTK  G++VL FAK++IF ++YF+MYLA+V++G  
Sbjct: 1232 TKGSRVDRAEEALAHMGSSVFSGITLTKFGGIVVLAFAKSQIFQIFYFRMYLAIVLLGAT 1252

BLAST of HG10000872 vs. ExPASy Swiss-Prot
Match: Q6T3U3 (NPC1-like intracellular cholesterol transporter 1 OS=Rattus norvegicus OX=10116 GN=Npc1l1 PE=1 SV=1)

HSP 1 Score: 421.4 bits (1082), Expect = 2.2e-116
Identity = 282/804 (35.07%), Postives = 449/804 (55.85%), Query Frame = 0

Query: 27   TCFSAFKAPLDPSTSLGGFVGNNYSEASAFVVTYPVNNAIDAVGNENGKAIAWEKAFVKL 86
            +C + + AP+ P  ++GG+ G +YSEA A ++T+ +NN   A      +A  WE+AF+K 
Sbjct: 541  SCMADYGAPVFPFLAVGGYQGTDYSEAEALIITFSLNN-YPADDPRMAQAKLWEEAFLK- 600

Query: 87   AKEELLPLVQSRNLTLSFSSESSIEEELKRESTADILTIAV------------------- 146
             + E      S    ++FS+E S+E+E+ R +  D+   AV                   
Sbjct: 601  -EMESFQRNTSDKFQVAFSAERSLEDEINRTTIQDLPVFAVSYIIVFLYISLALGSYSRC 660

Query: 147  ---------LLGLSGVILVVLSVLGSVGFFSAIGVKSTLIIMEVIPFLVLAVGVDNMCIL 206
                      LGL GVI+V+ +VL ++GF+S +GV S+L+I++V+PFLVLAVG DN+ I 
Sbjct: 661  SRVAVESKATLGLGGVIVVLGAVLAAMGFYSYLGVPSSLVIIQVVPFLVLAVGADNIFIF 720

Query: 207  VHAVKRQPYELTLEER---ISSALVEVGPSITLASLSEILAFAVGTFVPMPACRVFSMFA 266
            V   +R P  +  E+R   I   L  V PS+ L SLSE + F +G   PMPA R F++ +
Sbjct: 721  VLEYQRLP-RMPGEQREAHIGRTLGSVAPSMLLCSLSEAICFFLGALTPMPAVRTFALTS 780

Query: 267  ALAVLLDFILQLSAFVALIVFDILRAEDHRVD---CFPCIKVPPHSDELNQGFNQGRPGL 326
             LA++LDF+LQ++AFVAL+  D  R E  R D   CF   K+PP  +         + GL
Sbjct: 781  GLAIILDFLLQMTAFVALLSLDSKRQEASRPDVLCCFSTRKLPPPKE---------KEGL 840

Query: 327  LSRYMKDVHAPLLGLWGVKIVVVTIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQDYF 386
            L R+ + ++AP L    ++ VV+ +F+ L   ++ L   I VGL+Q++ LP+DSYL DYF
Sbjct: 841  LLRFFRKIYAPFLLHRFIRPVVMLLFLTLFGANLYLMCNINVGLDQELALPKDSYLIDYF 900

Query: 387  DDLAEYLRIGPPLYFV-VKDYNYSSKSRHTNQLCSISQCDSNSLLNEISRASLTPELNYI 446
              L  YL +GPP+YFV    +N+SS++   N  CS + C S SL  +I  AS  P+ +Y+
Sbjct: 901  LFLNRYLEVGPPVYFVTTSGFNFSSEA-GMNATCSSAGCKSFSLTQKIQYASEFPDQSYV 960

Query: 447  AKPAASWLDDFLVWLSPEAFGCCRKFTNG----SYCPPDDQPPCCFPDEGSCDSSEGLCK 506
            A  A+SW+DDF+ WL+P +  CCR +  G     +CP             S D+S    K
Sbjct: 961  AIAASSWVDDFIDWLTPSS-SCCRLYIRGPHKDEFCP-------------STDTSFNCLK 1020

Query: 507  DCTTVKFYFCYIVFITIKMAFGIWYGGCFHHSDLVADRPTTVQFQEKLPWFLNSLPSADC 566
            +C                            +  L   RPT  QF + LPWFLN  P+  C
Sbjct: 1021 NCM---------------------------NRTLGPVRPTAEQFHKYLPWFLNDPPNIRC 1080

Query: 567  AKGGHGAYTNSVNLNGYESGIIKASEFRSYHTPLNKQGDYVNALRAAKEFSSKISDSLK- 626
             KGG  AY  SVNL+    G + AS+F +YH PL    D+  ALRA++  ++ I+  L+ 
Sbjct: 1081 PKGGLAAYRTSVNLS--SDGQVIASQFMAYHKPLRNSQDFTEALRASRLLAANITADLRK 1140

Query: 627  -------IEIFPYSVFYIFFEQYLDIWKTALMNIAIALGAIFIVSLVITSSLWSSGII-I 686
                    E+FPY++  +F++QYL +    +  +A+     F+V  ++      SGI+ +
Sbjct: 1141 VPGTDPNFEVFPYTISNVFYQQYLTVLPEGIFTLALCFVPTFVVCYLLLGLDMCSGILNL 1200

Query: 687  LVLAMIIIDLMGVMAILKIQLNAVSVVNILMSIGIAVEFCVHLVHAFSVSC-GDKSQRAQ 746
            L + MI++D +G+MA+  I  NAVS++N++ ++G++VEF  H+  +F+VS    + +RA+
Sbjct: 1201 LSIIMILVDTIGLMAVWGISYNAVSLINLVTAVGMSVEFVSHITRSFAVSTKPTRLERAK 1260

Query: 747  EALSTMGASVFSGITLTKLVGVIVLCFAKTEIFVVYYFQMYLALVIIGFLHGLMFLPVIL 782
            +A   MG++VF+G+ +T   G+++L FA+ ++  +++F++ L + ++G LHGL+FLPV+L
Sbjct: 1261 DATVFMGSAVFAGVAMTNFPGILILGFAQAQLIQIFFFRLNLLITLLGLLHGLVFLPVVL 1287

BLAST of HG10000872 vs. ExPASy Swiss-Prot
Match: Q6T3U4 (NPC1-like intracellular cholesterol transporter 1 OS=Mus musculus OX=10090 GN=Npc1l1 PE=1 SV=1)

HSP 1 Score: 406.4 bits (1043), Expect = 7.4e-112
Identity = 274/800 (34.25%), Postives = 440/800 (55.00%), Query Frame = 0

Query: 27   TCFSAFKAPLDPSTSLGGFVGNNYSEASAFVVTYPVNNAIDAVGNENGKAIAWEKAFVKL 86
            +C + + AP+ P  ++GG+ G +YSEA A ++T+ +NN   A       A  WE+AF+K 
Sbjct: 541  SCIADYGAPVFPFLAVGGYQGTDYSEAEALIITFSINN-YPADDPRMAHAKLWEEAFLK- 600

Query: 87   AKEELLPLVQSRNLTLSFSSESSIEEELKRESTADILTIAV------------------- 146
             + +      +    ++FS+E S+E+E+ R +  D+   A+                   
Sbjct: 601  -EMQSFQRSTADKFQIAFSAERSLEDEINRTTIQDLPVFAISYLIVFLYISLALGSYSRW 660

Query: 147  ---------LLGLSGVILVVLSVLGSVGFFSAIGVKSTLIIMEVIPFLVLAVGVDNMCIL 206
                      LGL GV +V+ +V+ ++GF+S +GV S+L+I++V+PFLVLAVG DN+ I 
Sbjct: 661  SRVAVDSKATLGLGGVAVVLGAVVAAMGFYSYLGVPSSLVIIQVVPFLVLAVGADNIFIF 720

Query: 207  VHAVKRQPYELTLEER---ISSALVEVGPSITLASLSEILAFAVGTFVPMPACRVFSMFA 266
            V   +R P  +  E+R   I   L  V PS+ L SLSE + F +G    MPA R F++ +
Sbjct: 721  VLEYQRLP-RMPGEQREAHIGRTLGSVAPSMLLCSLSEAICFFLGALTSMPAVRTFALTS 780

Query: 267  ALAVLLDFILQLSAFVALIVFDILRAEDHRVD---CFPCIKVPPHSDELNQGFNQGRPGL 326
             LA++ DF+LQ++AFVAL+  D  R E  R D   CF    +PP            + GL
Sbjct: 781  GLAIIFDFLLQMTAFVALLSLDSKRQEASRPDVVCCFSSRNLPPPKQ---------KEGL 840

Query: 327  LSRYMKDVHAPLLGLWGVKIVVVTIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQDYF 386
            L  + + ++ P L    ++ VV+ +F+ L   ++ L   I VGL+Q + LP+DSYL DYF
Sbjct: 841  LLCFFRKIYTPFLLHRFIRPVVLLLFLVLFGANLYLMCNISVGLDQDLALPKDSYLIDYF 900

Query: 387  DDLAEYLRIGPPLYF-VVKDYNYSSKSRHTNQLCSISQCDSNSLLNEISRASLTPELNYI 446
              L  YL +GPP+YF     YN+S+++   N +CS + C+S SL  +I  AS  P  +Y+
Sbjct: 901  LFLNRYLEVGPPVYFDTTSGYNFSTEA-GMNAICSSAGCESFSLTQKIQYASEFPNQSYV 960

Query: 447  AKPAASWLDDFLVWLSPEAFGCCRKFTNG----SYCPPDDQPPCCFPDEGSCDSSEGLCK 506
            A  A+SW+DDF+ WL+P +  CCR +T G     +CP             S D+S    K
Sbjct: 961  AIAASSWVDDFIDWLTPSS-SCCRIYTRGPHKDEFCP-------------STDTSFNCLK 1020

Query: 507  DCTTVKFYFCYIVFITIKMAFGIWYGGCFHHSDLVADRPTTVQFQEKLPWFLNSLPSADC 566
            +C                            +  L   RPTT QF + LPWFLN  P+  C
Sbjct: 1021 NCM---------------------------NRTLGPVRPTTEQFHKYLPWFLNDTPNIRC 1080

Query: 567  AKGGHGAYTNSVNLNGYESGIIKASEFRSYHTPLNKQGDYVNALRAAKEFSSKISDSLK- 626
             KGG  AY  SVNL+    G I AS+F +YH PL    D+  ALRA++  ++ I+  L+ 
Sbjct: 1081 PKGGLAAYRTSVNLS--SDGQIIASQFMAYHKPLRNSQDFTEALRASRLLAANITAELRK 1140

Query: 627  -------IEIFPYSVFYIFFEQYLDIWKTALMNIAIALGAIFIVSLVITSSLWSSGII-I 686
                    E+FPY++  +F++QYL +    +  +A+     F+V  ++      SGI+ +
Sbjct: 1141 VPGTDPNFEVFPYTISNVFYQQYLTVLPEGIFTLALCFVPTFVVCYLLLGLDIRSGILNL 1200

Query: 687  LVLAMIIIDLMGVMAILKIQLNAVSVVNILMSIGIAVEFCVHLVHAFSVSC-GDKSQRAQ 746
            L + MI++D +G+MA+  I  NAVS++N++ ++G++VEF  H+  +F+VS    + +RA+
Sbjct: 1201 LSIIMILVDTIGLMAVWGISYNAVSLINLVTAVGMSVEFVSHITRSFAVSTKPTRLERAK 1260

Query: 747  EALSTMGASVFSGITLTKLVGVIVLCFAKTEIFVVYYFQMYLALVIIGFLHGLMFLPVIL 778
            +A   MG++VF+G+ +T   G+++L FA+ ++  +++F++ L + ++G LHGL+FLPV+L
Sbjct: 1261 DATIFMGSAVFAGVAMTNFPGILILGFAQAQLIQIFFFRLNLLITLLGLLHGLVFLPVVL 1283

BLAST of HG10000872 vs. ExPASy TrEMBL
Match: A0A5A7U0V7 (Niemann-Pick C1 protein-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold133G001120 PE=3 SV=1)

HSP 1 Score: 1350.9 bits (3495), Expect = 0.0e+00
Identity = 712/809 (88.01%), Postives = 738/809 (91.22%), Query Frame = 0

Query: 1    MDPENFDDYGGVEHAEYCFQHYASTETCFSAFKAPLDPSTSLGGFVGNNYSEASAFVVTY 60
            M+PENFDDYGGVEHAEYCFQHY S+ETCFSAFKAPLDPSTSLGGF G+NYSEASAFV+TY
Sbjct: 497  MNPENFDDYGGVEHAEYCFQHYTSSETCFSAFKAPLDPSTSLGGFFGSNYSEASAFVITY 556

Query: 61   PVNNAIDAVGNENGKAIAWEKAFVKLAKEELLPLVQSRNLTLSFSSESSIEEELKRESTA 120
            PVNNAIDAVGNENGKAIAWEKAFVKLAKEEL+PLV SRNLTLSFSSESSIEEELKRESTA
Sbjct: 557  PVNNAIDAVGNENGKAIAWEKAFVKLAKEELMPLVHSRNLTLSFSSESSIEEELKRESTA 616

Query: 121  DILTIA----------------------------VLLGLSGVILVVLSVLGSVGFFSAIG 180
            DILTIA                            VLLGLSGVILVVLSVLGSVGFFSAIG
Sbjct: 617  DILTIAVSYLVMFAYISVALGDSNISSSFYLSSKVLLGLSGVILVVLSVLGSVGFFSAIG 676

Query: 181  VKSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQPYELTLEERISSALVEVGPSITLASLS 240
            +KSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQPYEL+LE+RISSALVEVGPSITLASLS
Sbjct: 677  IKSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQPYELSLEDRISSALVEVGPSITLASLS 736

Query: 241  EILAFAVGTFVPMPACRVFSMFAALAVLLDFILQLSAFVALIVFDILRAEDHRVDCFPCI 300
            EILAFAVGTFVPMPACRVFS+FAALAVLLDFILQLSAFVALIV DILRAEDHRVDCFPCI
Sbjct: 737  EILAFAVGTFVPMPACRVFSLFAALAVLLDFILQLSAFVALIVLDILRAEDHRVDCFPCI 796

Query: 301  KVPPHSDELNQGFNQGRPGLLSRYMKDVHAPLLGLWGVKIVVVTIFVGLTLGSIALSTKI 360
            KV PHSDE NQGFNQGR GLLSRYMKDVHAPLLG WGVKIVVV IFVGLTLGSIALSTKI
Sbjct: 797  KVHPHSDEPNQGFNQGRHGLLSRYMKDVHAPLLGFWGVKIVVVVIFVGLTLGSIALSTKI 856

Query: 361  EVGLEQKIVLPRDSYLQDYFDDLAEYLRIGPPLYFVVKDYNYSSKSRHTNQLCSISQCDS 420
            EVGLEQKIVLPRDSYLQDYFDDLAEYLRIGPPLYFVVKDYNYSS+SR TNQLCSIS CDS
Sbjct: 857  EVGLEQKIVLPRDSYLQDYFDDLAEYLRIGPPLYFVVKDYNYSSRSRQTNQLCSISHCDS 916

Query: 421  NSLLNEISRASLTPELNYIAKPAASWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCC 480
            NSLLNEISRASLTPELNYIAKPAASWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCC
Sbjct: 917  NSLLNEISRASLTPELNYIAKPAASWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCC 976

Query: 481  FPDEGSCDSSEGLCKDCTTVKFYFCYIVFITIKMAFGIWYGGCFHHSDLVADRPTTVQFQ 540
            FPDEG CDSSEG+C+DCTT                       CF HSDLV DRPTT QF+
Sbjct: 977  FPDEGFCDSSEGVCRDCTT-----------------------CFRHSDLVGDRPTTGQFK 1036

Query: 541  EKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRSYHTPLNKQGDYVNALR 600
            EKLPWFLNSLPSADCAKGGHGAYTNSVNL GYE GIIKASEFRSYHTPLNKQGDYVNALR
Sbjct: 1037 EKLPWFLNSLPSADCAKGGHGAYTNSVNLKGYEIGIIKASEFRSYHTPLNKQGDYVNALR 1096

Query: 601  AAKEFSSKISDSLKIEIFPYSVFYIFFEQYLDIWKTALMNIAIALGAIFIVSLVITSSLW 660
            AAKEF SKISDSLK++IFPYSVFYIFFEQYLDIW TALMNIAIALGAIFIVSLVITSSLW
Sbjct: 1097 AAKEFCSKISDSLKMDIFPYSVFYIFFEQYLDIWNTALMNIAIALGAIFIVSLVITSSLW 1156

Query: 661  SSGIIILVLAMIIIDLMGVMAILKIQLNAVSVVNILMSIGIAVEFCVHLVHAFSVSCGDK 720
             SG+IILVLAMI+IDL+GVMAILKIQLNAVSVVNILMSIGIAVEFCVHLVHAFSVSCGD+
Sbjct: 1157 CSGMIILVLAMIVIDLLGVMAILKIQLNAVSVVNILMSIGIAVEFCVHLVHAFSVSCGDR 1216

Query: 721  SQRAQEALSTMGASVFSGITLTKLVGVIVLCFAKTEIFVVYYFQMYLALVIIGFLHGLMF 780
            +QRAQEALST+GASVFSGITLTKLVGV+VLCFAK+EIFVVYYFQMYLALVIIGFLHGL+F
Sbjct: 1217 NQRAQEALSTIGASVFSGITLTKLVGVVVLCFAKSEIFVVYYFQMYLALVIIGFLHGLVF 1276

Query: 781  LPVILSMIGPPSRYLVKDDAPMETELLVS 782
            LPVILSMIGPPSRYL+KDDAP+ETELLVS
Sbjct: 1277 LPVILSMIGPPSRYLIKDDAPVETELLVS 1282

BLAST of HG10000872 vs. ExPASy TrEMBL
Match: A0A6J1CI77 (Niemann-Pick C1 protein OS=Momordica charantia OX=3673 GN=LOC111011166 PE=3 SV=1)

HSP 1 Score: 1349.3 bits (3491), Expect = 0.0e+00
Identity = 708/809 (87.52%), Postives = 737/809 (91.10%), Query Frame = 0

Query: 1    MDPENFDDYGGVEHAEYCFQHYASTETCFSAFKAPLDPSTSLGGFVGNNYSEASAFVVTY 60
            MDPEN+DDYGGV+HAEYCFQHYASTETCFSAFKAPLDPSTSLGGF GNNYSEASAFVVTY
Sbjct: 515  MDPENYDDYGGVQHAEYCFQHYASTETCFSAFKAPLDPSTSLGGFSGNNYSEASAFVVTY 574

Query: 61   PVNNAIDAVGNENGKAIAWEKAFVKLAKEELLPLVQSRNLTLSFSSESSIEEELKRESTA 120
            PVNNAIDAVGNENGKA+AWEKAFVKL KEELLPLVQSRNLTLSFSSESSIEEELKRESTA
Sbjct: 575  PVNNAIDAVGNENGKAVAWEKAFVKLVKEELLPLVQSRNLTLSFSSESSIEEELKRESTA 634

Query: 121  DILTIA----------------------------VLLGLSGVILVVLSVLGSVGFFSAIG 180
            D+LTI                             VLLGLSGVILVVLSVLGSVGFFSAIG
Sbjct: 635  DVLTIVVSYLVMFVYISVALGDSSISSSFYLSSKVLLGLSGVILVVLSVLGSVGFFSAIG 694

Query: 181  VKSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQPYELTLEERISSALVEVGPSITLASLS 240
            VKSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQP+ELTLEERISSALVEVGPSITLASLS
Sbjct: 695  VKSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQPFELTLEERISSALVEVGPSITLASLS 754

Query: 241  EILAFAVGTFVPMPACRVFSMFAALAVLLDFILQLSAFVALIVFDILRAEDHRVDCFPCI 300
            EILAFAVG FVPMPACRVFSMFAALAVLLDFILQLSAFVALIVFDILRAEDHRVDCFPCI
Sbjct: 755  EILAFAVGAFVPMPACRVFSMFAALAVLLDFILQLSAFVALIVFDILRAEDHRVDCFPCI 814

Query: 301  KVPPHSDELNQGFNQGRPGLLSRYMKDVHAPLLGLWGVKIVVVTIFVGLTLGSIALSTKI 360
            KV PHSDE NQGFNQG+PGLLS YMKDVHAPLLGLWGVKI V+ IFVG TLG IALSTKI
Sbjct: 815  KVTPHSDEPNQGFNQGKPGLLSWYMKDVHAPLLGLWGVKIAVIVIFVGFTLGGIALSTKI 874

Query: 361  EVGLEQKIVLPRDSYLQDYFDDLAEYLRIGPPLYFVVKDYNYSSKSRHTNQLCSISQCDS 420
            EVGLEQKIVLPRDSYLQDYFDDLAEYLRIGPPLYFVVKDYNYSS+SR TNQLCSISQCDS
Sbjct: 875  EVGLEQKIVLPRDSYLQDYFDDLAEYLRIGPPLYFVVKDYNYSSESRQTNQLCSISQCDS 934

Query: 421  NSLLNEISRASLTPELNYIAKPAASWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCC 480
            NSLLNEISRASLTPELNYIAKPAASWLDDFLVWLSP+AFGCCRKFTNGSYCPPDDQPPCC
Sbjct: 935  NSLLNEISRASLTPELNYIAKPAASWLDDFLVWLSPDAFGCCRKFTNGSYCPPDDQPPCC 994

Query: 481  FPDEGSCDSSEGLCKDCTTVKFYFCYIVFITIKMAFGIWYGGCFHHSDLVADRPTTVQFQ 540
            FPDEGSCDSS G+CKDCTT                       CFH+SDLVA RPTT+QFQ
Sbjct: 995  FPDEGSCDSSGGVCKDCTT-----------------------CFHYSDLVAGRPTTIQFQ 1054

Query: 541  EKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRSYHTPLNKQGDYVNALR 600
            EKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFR+YHTPLNKQGDYVNALR
Sbjct: 1055 EKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRTYHTPLNKQGDYVNALR 1114

Query: 601  AAKEFSSKISDSLKIEIFPYSVFYIFFEQYLDIWKTALMNIAIALGAIFIVSLVITSSLW 660
            AAKEFSSK+SDSLK++IFPYSVFYIFFEQYLDIWK AL+NIA+ALGA+F+VSLVITS LW
Sbjct: 1115 AAKEFSSKMSDSLKMDIFPYSVFYIFFEQYLDIWKIALINIAVALGAVFVVSLVITSCLW 1174

Query: 661  SSGIIILVLAMIIIDLMGVMAILKIQLNAVSVVNILMSIGIAVEFCVHLVHAFSVSCGDK 720
            SSGIIILVLAMIIIDLMG+MAIL IQLNAVSVVN+LMSIGIAVEFCVH+VHAF VS GD+
Sbjct: 1175 SSGIIILVLAMIIIDLMGIMAILNIQLNAVSVVNLLMSIGIAVEFCVHIVHAFLVSSGDR 1234

Query: 721  SQRAQEALSTMGASVFSGITLTKLVGVIVLCFAKTEIFVVYYFQMYLALVIIGFLHGLMF 780
            SQRA+EAL TMGASVFSGITLTKLVGVIVLCF+++EIFVVYYFQMYLALVIIGFLHGL+F
Sbjct: 1235 SQRAREALGTMGASVFSGITLTKLVGVIVLCFSRSEIFVVYYFQMYLALVIIGFLHGLVF 1294

Query: 781  LPVILSMIGPPSRYLVKDDAPMETELLVS 782
            LPVILSMIGPPSRYLVKDDAP ETELLVS
Sbjct: 1295 LPVILSMIGPPSRYLVKDDAPTETELLVS 1300

BLAST of HG10000872 vs. ExPASy TrEMBL
Match: A0A6J1G6Q4 (Niemann-Pick C1 protein-like OS=Cucurbita moschata OX=3662 GN=LOC111451262 PE=3 SV=1)

HSP 1 Score: 1348.6 bits (3489), Expect = 0.0e+00
Identity = 714/809 (88.26%), Postives = 737/809 (91.10%), Query Frame = 0

Query: 1    MDPENFDDYGGVEHAEYCFQHYASTETCFSAFKAPLDPSTSLGGFVGNNYSEASAFVVTY 60
            MDPENFD+YGGVEH EYC QHY STETCFSAFKAPLDPSTSLGGFVGNNYSEASAFVVTY
Sbjct: 516  MDPENFDEYGGVEHVEYCLQHYTSTETCFSAFKAPLDPSTSLGGFVGNNYSEASAFVVTY 575

Query: 61   PVNNAIDAVGNENGKAIAWEKAFVKLAKEELLPLVQSRNLTLSFSSESSIEEELKRESTA 120
            PVNNAID VG+ENGKAIAWEKAFVKLAKEELLPLVQS+NLTLSFSSESSIEEELKRESTA
Sbjct: 576  PVNNAIDDVGDENGKAIAWEKAFVKLAKEELLPLVQSKNLTLSFSSESSIEEELKRESTA 635

Query: 121  DILTIA----------------------------VLLGLSGVILVVLSVLGSVGFFSAIG 180
            D+LTIA                            VLLGLSGV+LVVLSVLGSVGFFSA+G
Sbjct: 636  DVLTIAVSYLVMFAYISVALGDSNISSSFYLSSKVLLGLSGVLLVVLSVLGSVGFFSALG 695

Query: 181  VKSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQPYELTLEERISSALVEVGPSITLASLS 240
            VKSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQP+ELTLEERIS+ALVEVGPSITLASLS
Sbjct: 696  VKSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQPFELTLEERISNALVEVGPSITLASLS 755

Query: 241  EILAFAVGTFVPMPACRVFSMFAALAVLLDFILQLSAFVALIVFDILRAEDHRVDCFPCI 300
            EILAFAVGTFVPMPACRVFSMFAALAV LDFILQLSAFVALIVFDI RAE+HRVDCFPCI
Sbjct: 756  EILAFAVGTFVPMPACRVFSMFAALAVFLDFILQLSAFVALIVFDIRRAENHRVDCFPCI 815

Query: 301  KVPPHSDELNQGFNQGRPGLLSRYMKDVHAPLLGLWGVKIVVVTIFVGLTLGSIALSTKI 360
            KVPP SDE NQGFNQ R GLLSRYMKDVHAPLLGLWGVKI VV IFVGLTLGSIALSTKI
Sbjct: 816  KVPPRSDEPNQGFNQRRLGLLSRYMKDVHAPLLGLWGVKIAVVIIFVGLTLGSIALSTKI 875

Query: 361  EVGLEQKIVLPRDSYLQDYFDDLAEYLRIGPPLYFVVKDYNYSSKSRHTNQLCSISQCDS 420
            EVGLEQKIVLPRDSYLQ YFDDLAEYLRIGPPLYFVVKDYNYSSKSRHTNQLCSISQCDS
Sbjct: 876  EVGLEQKIVLPRDSYLQGYFDDLAEYLRIGPPLYFVVKDYNYSSKSRHTNQLCSISQCDS 935

Query: 421  NSLLNEISRASLTPELNYIAKPAASWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCC 480
            NSLLNEISRASLTPELNYIAKPAASWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCC
Sbjct: 936  NSLLNEISRASLTPELNYIAKPAASWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCC 995

Query: 481  FPDEGSCDSSEGLCKDCTTVKFYFCYIVFITIKMAFGIWYGGCFHHSDLVADRPTTVQFQ 540
             PDEG CD+SEG+C+DCTT                       CFHHSDLVA RPTTVQF+
Sbjct: 996  IPDEGFCDASEGVCQDCTT-----------------------CFHHSDLVAGRPTTVQFR 1055

Query: 541  EKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRSYHTPLNKQGDYVNALR 600
            EKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRSYHTPLNKQGDYVNALR
Sbjct: 1056 EKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRSYHTPLNKQGDYVNALR 1115

Query: 601  AAKEFSSKISDSLKIEIFPYSVFYIFFEQYLDIWKTALMNIAIALGAIFIVSLVITSSLW 660
            AAKEF SKISDSLK++IFPYSVFYIFFEQYLDIWKTALMNIAIALGAIFIVSLVITS LW
Sbjct: 1116 AAKEFCSKISDSLKMDIFPYSVFYIFFEQYLDIWKTALMNIAIALGAIFIVSLVITSCLW 1175

Query: 661  SSGIIILVLAMIIIDLMGVMAILKIQLNAVSVVNILMSIGIAVEFCVHLVHAFSVSCGDK 720
            SSGIIILVLAMIIIDLMGVMAIL IQLNAVSVVNILMSIGIAVEFCVHLVHAFSVSCGD+
Sbjct: 1176 SSGIIILVLAMIIIDLMGVMAILNIQLNAVSVVNILMSIGIAVEFCVHLVHAFSVSCGDR 1235

Query: 721  SQRAQEALSTMGASVFSGITLTKLVGVIVLCFAKTEIFVVYYFQMYLALVIIGFLHGLMF 780
            SQRA+EAL+TMGASVFSGITLTKLVGVIVLCFAK+EIFV+YYFQMYLALVIIGFLHGL+F
Sbjct: 1236 SQRAREALTTMGASVFSGITLTKLVGVIVLCFAKSEIFVIYYFQMYLALVIIGFLHGLVF 1295

Query: 781  LPVILSMIGPPSRYLVKDDAPMETELLVS 782
            LPVILSMIGPPSRY V+DDAP+ETEL VS
Sbjct: 1296 LPVILSMIGPPSRYSVQDDAPIETELHVS 1301

BLAST of HG10000872 vs. ExPASy TrEMBL
Match: A0A1S3BJR3 (Niemann-Pick C1 protein-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103490460 PE=3 SV=1)

HSP 1 Score: 1348.6 bits (3489), Expect = 0.0e+00
Identity = 711/809 (87.89%), Postives = 737/809 (91.10%), Query Frame = 0

Query: 1    MDPENFDDYGGVEHAEYCFQHYASTETCFSAFKAPLDPSTSLGGFVGNNYSEASAFVVTY 60
            M+PENFDDYGGVEHAEYCFQHY S+ETCFSAFKAPLDPSTSLGGF G+NYSEASAFV+TY
Sbjct: 516  MNPENFDDYGGVEHAEYCFQHYTSSETCFSAFKAPLDPSTSLGGFFGSNYSEASAFVITY 575

Query: 61   PVNNAIDAVGNENGKAIAWEKAFVKLAKEELLPLVQSRNLTLSFSSESSIEEELKRESTA 120
            PVNNAIDAVGNENGKAIAWEKAFVKLAKEEL+PLV SRNLTLSFSSESSIEEELKRESTA
Sbjct: 576  PVNNAIDAVGNENGKAIAWEKAFVKLAKEELMPLVHSRNLTLSFSSESSIEEELKRESTA 635

Query: 121  DILTIA----------------------------VLLGLSGVILVVLSVLGSVGFFSAIG 180
            DILTIA                            VLLGLSGVILVVLSVLGSVGFFSAIG
Sbjct: 636  DILTIAVSYLVMFAYISVALGDSNISSSFYLSSKVLLGLSGVILVVLSVLGSVGFFSAIG 695

Query: 181  VKSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQPYELTLEERISSALVEVGPSITLASLS 240
            +KSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQPYEL+LE+RISSALVEVGPSITLASLS
Sbjct: 696  IKSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQPYELSLEDRISSALVEVGPSITLASLS 755

Query: 241  EILAFAVGTFVPMPACRVFSMFAALAVLLDFILQLSAFVALIVFDILRAEDHRVDCFPCI 300
            EILAFAVGTFVPMPACRVFS+FAALAVLLDFILQLSAFVALIV DILRAEDHRVDCFPCI
Sbjct: 756  EILAFAVGTFVPMPACRVFSLFAALAVLLDFILQLSAFVALIVLDILRAEDHRVDCFPCI 815

Query: 301  KVPPHSDELNQGFNQGRPGLLSRYMKDVHAPLLGLWGVKIVVVTIFVGLTLGSIALSTKI 360
            KV PHSDE NQGFNQGR GLLS YMKDVHAPLLG WGVKIVVV IFVGLTLGSIALSTKI
Sbjct: 816  KVHPHSDEPNQGFNQGRHGLLSLYMKDVHAPLLGFWGVKIVVVVIFVGLTLGSIALSTKI 875

Query: 361  EVGLEQKIVLPRDSYLQDYFDDLAEYLRIGPPLYFVVKDYNYSSKSRHTNQLCSISQCDS 420
            EVGLEQKIVLPRDSYLQDYFDDLAEYLRIGPPLYFVVKDYNYSS+SR TNQLCSIS CDS
Sbjct: 876  EVGLEQKIVLPRDSYLQDYFDDLAEYLRIGPPLYFVVKDYNYSSRSRQTNQLCSISHCDS 935

Query: 421  NSLLNEISRASLTPELNYIAKPAASWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCC 480
            NSLLNEISRASLTPELNYIAKPAASWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCC
Sbjct: 936  NSLLNEISRASLTPELNYIAKPAASWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCC 995

Query: 481  FPDEGSCDSSEGLCKDCTTVKFYFCYIVFITIKMAFGIWYGGCFHHSDLVADRPTTVQFQ 540
            FPDEG CDSSEG+C+DCTT                       CF HSDLV DRPTT QF+
Sbjct: 996  FPDEGFCDSSEGVCRDCTT-----------------------CFRHSDLVGDRPTTEQFK 1055

Query: 541  EKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRSYHTPLNKQGDYVNALR 600
            EKLPWFLNSLPSADCAKGGHGAYTNSVNL GYE GIIKASEFRSYHTPLNKQGDYVNALR
Sbjct: 1056 EKLPWFLNSLPSADCAKGGHGAYTNSVNLKGYEIGIIKASEFRSYHTPLNKQGDYVNALR 1115

Query: 601  AAKEFSSKISDSLKIEIFPYSVFYIFFEQYLDIWKTALMNIAIALGAIFIVSLVITSSLW 660
            AAKEF SKISDSLK++IFPYSVFYIFFEQYLDIW TALMNIAIALGAIFIVSLVITSSLW
Sbjct: 1116 AAKEFCSKISDSLKMDIFPYSVFYIFFEQYLDIWNTALMNIAIALGAIFIVSLVITSSLW 1175

Query: 661  SSGIIILVLAMIIIDLMGVMAILKIQLNAVSVVNILMSIGIAVEFCVHLVHAFSVSCGDK 720
             SG+IILVLAMI+IDL+GVMAILKIQLNAVSVVNILMSIGIAVEFCVHLVHAFSVSCGD+
Sbjct: 1176 CSGMIILVLAMIVIDLLGVMAILKIQLNAVSVVNILMSIGIAVEFCVHLVHAFSVSCGDR 1235

Query: 721  SQRAQEALSTMGASVFSGITLTKLVGVIVLCFAKTEIFVVYYFQMYLALVIIGFLHGLMF 780
            +QRAQEALST+GASVFSGITLTKLVGV+VLCFAK+EIFVVYYFQMYLALVIIGFLHGL+F
Sbjct: 1236 NQRAQEALSTIGASVFSGITLTKLVGVVVLCFAKSEIFVVYYFQMYLALVIIGFLHGLVF 1295

Query: 781  LPVILSMIGPPSRYLVKDDAPMETELLVS 782
            LPVILSMIGPPSRYL+KDDAP+ETELLVS
Sbjct: 1296 LPVILSMIGPPSRYLIKDDAPVETELLVS 1301

BLAST of HG10000872 vs. ExPASy TrEMBL
Match: A0A6J1I473 (Niemann-Pick C1 protein-like OS=Cucurbita maxima OX=3661 GN=LOC111469740 PE=3 SV=1)

HSP 1 Score: 1348.2 bits (3488), Expect = 0.0e+00
Identity = 713/809 (88.13%), Postives = 736/809 (90.98%), Query Frame = 0

Query: 1    MDPENFDDYGGVEHAEYCFQHYASTETCFSAFKAPLDPSTSLGGFVGNNYSEASAFVVTY 60
            MDPENFD+YGGVEH EYC QHY STETCFSAFKAPLDPSTSLGGFVGNNYSEASAFVVTY
Sbjct: 516  MDPENFDEYGGVEHVEYCLQHYTSTETCFSAFKAPLDPSTSLGGFVGNNYSEASAFVVTY 575

Query: 61   PVNNAIDAVGNENGKAIAWEKAFVKLAKEELLPLVQSRNLTLSFSSESSIEEELKRESTA 120
            PVNNAID VG+ENGKAIAWEKAFVKL KEELLPLVQS+NLTLSFSSESSIEEELKRESTA
Sbjct: 576  PVNNAIDDVGDENGKAIAWEKAFVKLVKEELLPLVQSKNLTLSFSSESSIEEELKRESTA 635

Query: 121  DILTIA----------------------------VLLGLSGVILVVLSVLGSVGFFSAIG 180
            D+LTIA                            VLLGLSGV+LVVLSVLGSVGFFSA+G
Sbjct: 636  DVLTIAVSYLVMFAYISVALGDSNISSSFYLSSKVLLGLSGVLLVVLSVLGSVGFFSALG 695

Query: 181  VKSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQPYELTLEERISSALVEVGPSITLASLS 240
            VKSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQP+ELTLEERIS+ALVEVGPSITLASLS
Sbjct: 696  VKSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQPFELTLEERISNALVEVGPSITLASLS 755

Query: 241  EILAFAVGTFVPMPACRVFSMFAALAVLLDFILQLSAFVALIVFDILRAEDHRVDCFPCI 300
            EILAFAVGTFVPMPACRVFSMFAALAV LDFILQLSAFVALIVFDILRAE+HRVDCFPCI
Sbjct: 756  EILAFAVGTFVPMPACRVFSMFAALAVFLDFILQLSAFVALIVFDILRAENHRVDCFPCI 815

Query: 301  KVPPHSDELNQGFNQGRPGLLSRYMKDVHAPLLGLWGVKIVVVTIFVGLTLGSIALSTKI 360
            KVPP SDE NQGFNQ R GLLSRYMKDVHAPLLGLWGVKI VV IFVGLTLGSI LSTKI
Sbjct: 816  KVPPRSDEPNQGFNQRRLGLLSRYMKDVHAPLLGLWGVKIAVVIIFVGLTLGSITLSTKI 875

Query: 361  EVGLEQKIVLPRDSYLQDYFDDLAEYLRIGPPLYFVVKDYNYSSKSRHTNQLCSISQCDS 420
            EVGLEQKIVLPRDSYLQ YFDDLAEYLRIGPPLYFVVKDYNYSSKSRHTNQLCSISQCDS
Sbjct: 876  EVGLEQKIVLPRDSYLQGYFDDLAEYLRIGPPLYFVVKDYNYSSKSRHTNQLCSISQCDS 935

Query: 421  NSLLNEISRASLTPELNYIAKPAASWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCC 480
            NSLLNEISRASLTPELNYIAKPAASWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCC
Sbjct: 936  NSLLNEISRASLTPELNYIAKPAASWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCC 995

Query: 481  FPDEGSCDSSEGLCKDCTTVKFYFCYIVFITIKMAFGIWYGGCFHHSDLVADRPTTVQFQ 540
             PDEG CD+SEG+C+DCTT                       CFHHSDLVA RPTTVQF+
Sbjct: 996  IPDEGFCDASEGVCQDCTT-----------------------CFHHSDLVAGRPTTVQFR 1055

Query: 541  EKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRSYHTPLNKQGDYVNALR 600
            EKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRSYHTPLNKQGDYVNALR
Sbjct: 1056 EKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRSYHTPLNKQGDYVNALR 1115

Query: 601  AAKEFSSKISDSLKIEIFPYSVFYIFFEQYLDIWKTALMNIAIALGAIFIVSLVITSSLW 660
            AAKEF SKISDSLK++IFPYSVFYIFFEQYLDIWKTALMNIAIALGAIFIVSLVITS LW
Sbjct: 1116 AAKEFCSKISDSLKMDIFPYSVFYIFFEQYLDIWKTALMNIAIALGAIFIVSLVITSCLW 1175

Query: 661  SSGIIILVLAMIIIDLMGVMAILKIQLNAVSVVNILMSIGIAVEFCVHLVHAFSVSCGDK 720
            SSGIIILVLAMIIIDLMGVMAIL IQLNAVSVVNILMSIGIAVEFCVHLVHAFSVSCGD+
Sbjct: 1176 SSGIIILVLAMIIIDLMGVMAILNIQLNAVSVVNILMSIGIAVEFCVHLVHAFSVSCGDR 1235

Query: 721  SQRAQEALSTMGASVFSGITLTKLVGVIVLCFAKTEIFVVYYFQMYLALVIIGFLHGLMF 780
            SQR+QEAL+TMGASVFSGITLTKLVGVIVLCFAK+EIFV+YYFQMYLALVIIGFLHGL+F
Sbjct: 1236 SQRSQEALTTMGASVFSGITLTKLVGVIVLCFAKSEIFVIYYFQMYLALVIIGFLHGLVF 1295

Query: 781  LPVILSMIGPPSRYLVKDDAPMETELLVS 782
            LPVILSMIGPPSRY V+DDAP+ETEL VS
Sbjct: 1296 LPVILSMIGPPSRYSVQDDAPIETELHVS 1301

BLAST of HG10000872 vs. TAIR 10
Match: AT4G38350.1 (Patched family protein )

HSP 1 Score: 1128.2 bits (2917), Expect = 0.0e+00
Identity = 589/791 (74.46%), Postives = 667/791 (84.32%), Query Frame = 0

Query: 1    MDPENFDDYGGVEHAEYCFQHYASTETCFSAFKAPLDPSTSLGGFVGNNYSEASAFVVTY 60
            MD   FDDYGGVEHAEYCFQHY S+ETC SAF+AP+DPS  LGGF GNNYSEA+AFVVTY
Sbjct: 489  MDSGTFDDYGGVEHAEYCFQHYTSSETCLSAFQAPVDPSAVLGGFSGNNYSEATAFVVTY 548

Query: 61   PVNNAIDAVGNENGKAIAWEKAFVKLAKEELLPLVQSRNLTLSFSSESSIEEELKRESTA 120
            PVNN I    NEN +A+AWEK+F++LAKEELLP+V+S+NL+LSFSSESSIEEELKRESTA
Sbjct: 549  PVNNVIGDSSNENARAVAWEKSFIQLAKEELLPMVRSKNLSLSFSSESSIEEELKRESTA 608

Query: 121  DILTIA----------------------------VLLGLSGVILVVLSVLGSVGFFSAIG 180
            D++TIA                            VLLGLSGV+LV+LSVLGSVG FSA+G
Sbjct: 609  DVITIAASYLVMFVYISVTLGDAPQFYTFYISSKVLLGLSGVVLVLLSVLGSVGVFSALG 668

Query: 181  VKSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQPYELTLEERISSALVEVGPSITLASLS 240
            VKSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQP E++LE+RISSALVEVGPSITLASLS
Sbjct: 669  VKSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQPREVSLEQRISSALVEVGPSITLASLS 728

Query: 241  EILAFAVGTFVPMPACRVFSMFAALAVLLDFILQLSAFVALIVFDILRAEDHRVDCFPCI 300
            E+LAFAVG FVPMPACR+FSMFAALA++LDF LQ++AFVALIVFD  R+ D+R+DCFPCI
Sbjct: 729  EVLAFAVGAFVPMPACRIFSMFAALAIMLDFFLQITAFVALIVFDCKRSADNRIDCFPCI 788

Query: 301  KVPPHSDELNQGFNQGRPGLLSRYMKDVHAPLLGLWGVKIVVVTIFVGLTLGSIALSTKI 360
            KVP  S E  +G  +  PG L RYMK+VHAP+LGLWGVK+VVV +F    L SIA+S ++
Sbjct: 789  KVPSSSRESVEGGRE--PGFLERYMKEVHAPVLGLWGVKMVVVAVFFAFALASIAISPRL 848

Query: 361  EVGLEQKIVLPRDSYLQDYFDDLAEYLRIGPPLYFVVKDYNYSSKSRHTNQLCSISQCDS 420
            E GLEQKIVLPRDSYLQDYFD L+EYLR+GPPLYFVVK+YNYSS+SRHTNQLCSISQC+S
Sbjct: 849  ETGLEQKIVLPRDSYLQDYFDSLSEYLRVGPPLYFVVKNYNYSSESRHTNQLCSISQCNS 908

Query: 421  NSLLNEISRASLTPELNYIAKPAASWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCC 480
            NSLLNEISRAS   + +YIAKPAASWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCC
Sbjct: 909  NSLLNEISRASQASDTSYIAKPAASWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCC 968

Query: 481  FPDEGSCDSSEGLCKDCTTVKFYFCYIVFITIKMAFGIWYGGCFHHSDLVADRPTTVQFQ 540
              +E  C S +G+CKDCTT                       CF HSDLV DRP+T QF+
Sbjct: 969  TAEEDIC-SLDGICKDCTT-----------------------CFRHSDLVQDRPSTAQFR 1028

Query: 541  EKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRSYHTPLNKQGDYVNALR 600
            EKLPWFLN+LPSADCAKGGHGAYTNSV+L GYESG+I+ASEFR+YHTPLN QGDYVNALR
Sbjct: 1029 EKLPWFLNALPSADCAKGGHGAYTNSVDLKGYESGVIQASEFRTYHTPLNTQGDYVNALR 1088

Query: 601  AAKEFSSKISDSLKIEIFPYSVFYIFFEQYLDIWKTALMNIAIALGAIFIVSLVITSSLW 660
            AA+EFSS+IS+SLKI+IFPYSVFYIFFEQYL+IW  AL N+AIA+GAIFIV  +ITSS W
Sbjct: 1089 AAREFSSRISNSLKIDIFPYSVFYIFFEQYLNIWTVALTNLAIAIGAIFIVCWLITSSAW 1148

Query: 661  SSGIIILVLAMIIIDLMGVMAILKIQLNAVSVVNILMSIGIAVEFCVHLVHAFSVSCGDK 720
            SS II+LVL MI++DLMG+M IL IQLNAVSVVN++MSIGIAVEFCVH+ HAF +S GD+
Sbjct: 1149 SSAIIVLVLVMILVDLMGMMVILGIQLNAVSVVNLIMSIGIAVEFCVHISHAFLMSSGDR 1208

Query: 721  SQRAQEALSTMGASVFSGITLTKLVGVIVLCFAKTEIFVVYYFQMYLALVIIGFLHGLMF 764
              RA+EAL TMGASVFSGITLTKLVGVIVLCFA++EIFVVYYFQMYLALVIIGFLHGL+F
Sbjct: 1209 EHRAREALETMGASVFSGITLTKLVGVIVLCFARSEIFVVYYFQMYLALVIIGFLHGLVF 1253

BLAST of HG10000872 vs. TAIR 10
Match: AT4G38350.2 (Patched family protein )

HSP 1 Score: 1114.8 bits (2882), Expect = 0.0e+00
Identity = 589/815 (72.27%), Postives = 667/815 (81.84%), Query Frame = 0

Query: 1    MDPENFDDYGGVEHAEYCFQHYASTETCFSAFKAPLDPSTSLGGFVGNNYSE-------- 60
            MD   FDDYGGVEHAEYCFQHY S+ETC SAF+AP+DPS  LGGF GNNYSE        
Sbjct: 489  MDSGTFDDYGGVEHAEYCFQHYTSSETCLSAFQAPVDPSAVLGGFSGNNYSEVMVSELGC 548

Query: 61   ----------------ASAFVVTYPVNNAIDAVGNENGKAIAWEKAFVKLAKEELLPLVQ 120
                            A+AFVVTYPVNN I    NEN +A+AWEK+F++LAKEELLP+V+
Sbjct: 549  SVPFDCYSDVKRTLFQATAFVVTYPVNNVIGDSSNENARAVAWEKSFIQLAKEELLPMVR 608

Query: 121  SRNLTLSFSSESSIEEELKRESTADILTIA----------------------------VL 180
            S+NL+LSFSSESSIEEELKRESTAD++TIA                            VL
Sbjct: 609  SKNLSLSFSSESSIEEELKRESTADVITIAASYLVMFVYISVTLGDAPQFYTFYISSKVL 668

Query: 181  LGLSGVILVVLSVLGSVGFFSAIGVKSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQPYE 240
            LGLSGV+LV+LSVLGSVG FSA+GVKSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQP E
Sbjct: 669  LGLSGVVLVLLSVLGSVGVFSALGVKSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQPRE 728

Query: 241  LTLEERISSALVEVGPSITLASLSEILAFAVGTFVPMPACRVFSMFAALAVLLDFILQLS 300
            ++LE+RISSALVEVGPSITLASLSE+LAFAVG FVPMPACR+FSMFAALA++LDF LQ++
Sbjct: 729  VSLEQRISSALVEVGPSITLASLSEVLAFAVGAFVPMPACRIFSMFAALAIMLDFFLQIT 788

Query: 301  AFVALIVFDILRAEDHRVDCFPCIKVPPHSDELNQGFNQGRPGLLSRYMKDVHAPLLGLW 360
            AFVALIVFD  R+ D+R+DCFPCIKVP  S E  +G  +  PG L RYMK+VHAP+LGLW
Sbjct: 789  AFVALIVFDCKRSADNRIDCFPCIKVPSSSRESVEGGRE--PGFLERYMKEVHAPVLGLW 848

Query: 361  GVKIVVVTIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQDYFDDLAEYLRIGPPLYFV 420
            GVK+VVV +F    L SIA+S ++E GLEQKIVLPRDSYLQDYFD L+EYLR+GPPLYFV
Sbjct: 849  GVKMVVVAVFFAFALASIAISPRLETGLEQKIVLPRDSYLQDYFDSLSEYLRVGPPLYFV 908

Query: 421  VKDYNYSSKSRHTNQLCSISQCDSNSLLNEISRASLTPELNYIAKPAASWLDDFLVWLSP 480
            VK+YNYSS+SRHTNQLCSISQC+SNSLLNEISRAS   + +YIAKPAASWLDDFLVWLSP
Sbjct: 909  VKNYNYSSESRHTNQLCSISQCNSNSLLNEISRASQASDTSYIAKPAASWLDDFLVWLSP 968

Query: 481  EAFGCCRKFTNGSYCPPDDQPPCCFPDEGSCDSSEGLCKDCTTVKFYFCYIVFITIKMAF 540
            EAFGCCRKFTNGSYCPPDDQPPCC  +E  C S +G+CKDCTT                 
Sbjct: 969  EAFGCCRKFTNGSYCPPDDQPPCCTAEEDIC-SLDGICKDCTT----------------- 1028

Query: 541  GIWYGGCFHHSDLVADRPTTVQFQEKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGI 600
                  CF HSDLV DRP+T QF+EKLPWFLN+LPSADCAKGGHGAYTNSV+L GYESG+
Sbjct: 1029 ------CFRHSDLVQDRPSTAQFREKLPWFLNALPSADCAKGGHGAYTNSVDLKGYESGV 1088

Query: 601  IKASEFRSYHTPLNKQGDYVNALRAAKEFSSKISDSLKIEIFPYSVFYIFFEQYLDIWKT 660
            I+ASEFR+YHTPLN QGDYVNALRAA+EFSS+IS+SLKI+IFPYSVFYIFFEQYL+IW  
Sbjct: 1089 IQASEFRTYHTPLNTQGDYVNALRAAREFSSRISNSLKIDIFPYSVFYIFFEQYLNIWTV 1148

Query: 661  ALMNIAIALGAIFIVSLVITSSLWSSGIIILVLAMIIIDLMGVMAILKIQLNAVSVVNIL 720
            AL N+AIA+GAIFIV  +ITSS WSS II+LVL MI++DLMG+M IL IQLNAVSVVN++
Sbjct: 1149 ALTNLAIAIGAIFIVCWLITSSAWSSAIIVLVLVMILVDLMGMMVILGIQLNAVSVVNLI 1208

Query: 721  MSIGIAVEFCVHLVHAFSVSCGDKSQRAQEALSTMGASVFSGITLTKLVGVIVLCFAKTE 764
            MSIGIAVEFCVH+ HAF +S GD+  RA+EAL TMGASVFSGITLTKLVGVIVLCFA++E
Sbjct: 1209 MSIGIAVEFCVHISHAFLMSSGDREHRAREALETMGASVFSGITLTKLVGVIVLCFARSE 1268

BLAST of HG10000872 vs. TAIR 10
Match: AT1G42470.1 (Patched family protein )

HSP 1 Score: 972.6 bits (2513), Expect = 1.9e-283
Identity = 498/714 (69.75%), Postives = 594/714 (83.19%), Query Frame = 0

Query: 1    MDPENFDDYGGVEHAEYCFQHYASTETCFSAFKAPLDPSTSLGGFVGNNYSEASAFVVTY 60
            M PEN+DDYGGV+H +YCF+H+ STE+C SAFK PLDP+T+LGGF GN++SEASAF+VTY
Sbjct: 476  MKPENYDDYGGVDHVKYCFEHFTSTESCLSAFKGPLDPTTALGGFSGNSFSEASAFLVTY 535

Query: 61   PVNNAIDAVGNENGKAIAWEKAFVKLAKEELLPLVQSRNLTLSFSSESSIEEELKRESTA 120
            PV+N +D  GN+  KA+AWEKAF++LAK+ELLP+VQ++NLTLSFSSESSIEEELKRESTA
Sbjct: 536  PVDNFVDNKGNKTEKAVAWEKAFIQLAKDELLPMVQAKNLTLSFSSESSIEEELKRESTA 595

Query: 121  DILTIA----------------------------VLLGLSGVILVVLSVLGSVGFFSAIG 180
            D++TIA                            VLLGLSGV+LV+LSVLGSVGFFSA+G
Sbjct: 596  DVITIAISYLVMFAYISLTLGDSPRLKSFYITSKVLLGLSGVLLVMLSVLGSVGFFSAVG 655

Query: 181  VKSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQPYELTLEERISSALVEVGPSITLASLS 240
            +KSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQ  EL LE RIS+AL+EVGPSITLASL+
Sbjct: 656  MKSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQEQELPLERRISNALMEVGPSITLASLA 715

Query: 241  EILAFAVGTFVPMPACRVFSMFAALAVLLDFILQLSAFVALIVFDILRAEDHRVDCFPCI 300
            EILAFAVG F+ MPA RVFSMFAALAVLLDF+LQ++AFVALIVFD  R ED RVDCFPCI
Sbjct: 716  EILAFAVGAFIKMPAVRVFSMFAALAVLLDFLLQITAFVALIVFDFRRTEDKRVDCFPCI 775

Query: 301  KVPPHSDELNQGFNQGRPGLLSRYMKDVHAPLLGLWGVKIVVVTIFVGLTLGSIALSTKI 360
            K    S    +G  Q + GLL+RYMK+VHAP+L  W VKIVV+  F GL +  IALST+I
Sbjct: 776  KTSKSSISAEKGVGQRKAGLLTRYMKEVHAPVLSHWIVKIVVIAFFFGLAMAGIALSTRI 835

Query: 361  EVGLEQKIVLPRDSYLQDYFDDLAEYLRIGPPLYFVVKDYNYSSKSRHTNQLCSISQCDS 420
            E GLEQ+IVLP+DSYLQ YF++++ YLRIGPPLYFV+K+YNYSS+SRHTNQLCSI++C+ 
Sbjct: 836  EPGLEQQIVLPQDSYLQGYFNNISTYLRIGPPLYFVLKNYNYSSESRHTNQLCSINKCNP 895

Query: 421  NSLLNEISRASLTPELNYIAKPAASWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCC 480
            NSLLNEI+RASLTPEL+YIAKPAASWLDDFLVWLSPEAFGCCRKFTNG++CPPDDQPPCC
Sbjct: 896  NSLLNEIARASLTPELSYIAKPAASWLDDFLVWLSPEAFGCCRKFTNGTFCPPDDQPPCC 955

Query: 481  FPDEGSCDSSEGLCKDCTTVKFYFCYIVFITIKMAFGIWYGGCFHHSDLVADRPTTVQFQ 540
             P + SC  SE +CKDCTT                       CF H+DL +DRP+T QF+
Sbjct: 956  PPGQTSCGLSE-VCKDCTT-----------------------CFRHADLSSDRPSTTQFK 1015

Query: 541  EKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRSYHTPLNKQGDYVNALR 600
            EKLPWFLN+LPSADCAKGGHGAY++SV+L GY +GII+AS FR+YHTPLNKQ D+VN++R
Sbjct: 1016 EKLPWFLNALPSADCAKGGHGAYSSSVDLQGYANGIIQASSFRTYHTPLNKQVDFVNSMR 1075

Query: 601  AAKEFSSKISDSLKIEIFPYSVFYIFFEQYLDIWKTALMNIAIALGAIFIVSLVITSSLW 660
            AA+EFS+K+S SLK+EI+PYSVFY+FFEQYLDIWKTAL+N++IA+ A+F+V L+IT S W
Sbjct: 1076 AAQEFSAKVSRSLKMEIYPYSVFYMFFEQYLDIWKTALINLSIAIAAVFVVCLIITCSFW 1135

Query: 661  SSGIIILVLAMIIIDLMGVMAILKIQLNAVSVVNILMSIGIAVEFCVHLVHAFS 687
            SS II+LV+AMIIIDL+GVMA+  IQLNA+SVVN++MS+GIAVEFCVH+ HAFS
Sbjct: 1136 SSAIILLVIAMIIIDLLGVMAVFHIQLNALSVVNLIMSVGIAVEFCVHITHAFS 1165