Cucsat.G9521 (gene) Cucumber (B10) v3

Overview
NameCucsat.G9521
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionFormin-like protein
Locationctg1673: 1896862 .. 1899842 (-)
RNA-Seq ExpressionCucsat.G9521
SyntenyCucsat.G9521
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AGATATAATAAATAAATGGATGAATATAGAATATAGAATTTAGAAAGGTAAAGGGAAAGGGAAAGGGAAAAGGAAGGATTGAAGAGAAACAACAACAACTAACTGCTCACTTTGAAACTGTCAGCCACCAAACTCCAAAACATTAACACATAAACTGATGTTTTCTTTACAATCATTCTCTTCCAATCTTTCCATTTCTTCATTTCTCTCCGTTTCCCATCTCCTAATCCTCCCATGGATTCTCTATCGCACCTTATTCTCTTCCTCTTCTTCCTTTCTCTTTCCCCTCTTTCCTACTCCCAACTCAGCCCCCCTCAAAATATCGAAACTTACTACCCTTTCCCTCAACCGCCCTCGCCCTCCTCTTCTTCAGTTGACCATCCGCCATCGTCCACGTCCACCAAGACCATCGCCACCGCGGTGGCCGTCACTGCTGTCGGTGTTGCTCTGATTTCCACCTTCTTCTTCTTTTTAATACAGAGATATGTTATCGGCAGAAAGAGGAAAACAGAGGTCGTAAATTCAGGGACGGGTTCGGGTTCGGGTTCGGCAGTGCCACCGGCGGTTGCTCAGAGTGAATTTTCGCGGGTTGATGGGAATCTTAAAGGCTTTATTGTGGATGAAAATGGGTTGGATGTGATTTATTGGAAGAAACTTGAACAAAGGAAATCCAAAAATAGCTTTGATCGGGATGTTGAAGGTAATGTTAAGGAAAACCGAACAAAAAAGTCTGAGCCTGTTCAAGAAATTCCTCTGCTTCGAGGAAAATCTTCAACTTCGCATGTTAAAATTGCACCCGAAGATGAAGACGATAGTCGGATTACATCGCCACCGCCTCCTCCGCCGCCCCAGGTTAACCAACCTCCGCAATTTACTGCGATTTCGGTTCAGGCTGTTGGAAAATCACCAAGTAGTTCAAATCTTTCATCAACAGCACCAACGCAATCGACGGGAAATCAGGTACCTCCGAAACAATCTCCAATGGCCGTTCCGCCACCGCCCCCACCTATTCCAGCCAAGACCAATTCAAGACTGCCGCCGCCGCCACCTCCCATTCCCAAGACCAATTCAAGACCGCCGCCACCGCCGCCTCCTATTCAACCCAAGACCAACTCCGCAGGACCACCTCCTCCTCCTATACCGGCCAAGGCAAATCCGTCAGCACCACCGCCACCACCGCCCAAGGCCGGCGGTTCAAAATTACCCTTAAGGCCTGCACCTCCAAAAGAGAGTAACAAATCTTCTGCAGAGGCTTCTTCATCAGCTGACAATGGTCAGGTTAAAATGAAGCCCCTGCATTGGGATAAAGTGAACACTGCCAATGCCGATCATTCCATGGTTTGGGACAAAATGACCGCCGGTTCTTTCAAGTAATTATCTTTTCAAACACTCTTTCTTGTTTCTTGAATAATCTCTTTTGGGTACTGATTAGTTTTTCGTTTTGAGTACAGATTCGATGGGGATCTTATGGAAGCTCTCTTTGGATATGTCGCAACCAACCGGAAATCCCCCCGGAGTGAAGCTAGTTCTTCAGCAATCGCCGTTGGCCGGAACTCGGGGCCGTCGCAAACTTTCATTCTTGAACCGAAAAAATCTCAGAACATCGCCATTGTAATCAAGTCCTTAACAGTTCCTCGCAACGAAATCCTCGATGCACTCAACGAAGGTCAAGGCCTGGAAACAGAGGTTCTTGAAAAACTCACAAGAATTGCTCTGACTCAAGAAGAAATTTCCCAAATTCTTGCTTACAAAGGAGATCCCCAGAAGCTAGCTGATGCTGAATCTTTCCTTTATGATCTTCTCAAATCGGTTCCATCGGCCTTTACGCGCTTCAACGCCATGCTTTTCCGATTGAATTTTACGTCAGATATTCTCCATCACAAGGAATCTCTACAAACTCTGGAATCCGCTTGCAAGGAGCTTAGGACACGAGGATTGTTTATGAAACTTCTTGAAGCAATTCTCAAAGCTGGGAACCGGTTGAATGCAGGAACTGCAAGAGGAAATGCCAGAGCTTTCAATCTTACAGCTCTTCGAAAGCTCTCTGATGTTCGAAGCACTGATGGGAAAACCACTTTGCTTCATTTTGTAGTCCAGGAAGTGATCAGGGCAGAAGGGAAAAGGTGTGTTTTAAACAGGAACAAGAGCTTGAGCCGTAATAGCAGCCGTAGCAGCGACAACAGCTTTAGCAGCTTAGAAAACTCGGCTGCGAAGGAGGACAGAGTGAAGGAATACATGATGTTGGGATTGCCAGTGGTGGGAGGTCTAAGCTCCGAATTCTCTCATGTAAAGAAAGCGTCGGCGATCGACTACGAGAGCTTCGTCAAGGCAGGGACGTCCCTGACTAGTCGAACCGAAGAAATCCGGAAGCTCCTGACTCAAATGGGGAACAATGAAGGTGGGTTTGCGAAGGAAATGAGAGAATTTCTTGATGCAGCAGAGAATGAGCTGAAGATGGTAAGAGAAGCACAAACGAAGGTGATGGATTTAGTGATGAAGACAACAGAGTATTATCAGGCAGGAAGTTCTAAAGATAAAGAAACAAATCGGCTTCAATTGTTTATTATAATTAAAGATTTTCTAGAAATGGTAGATCGAGTTTGTGTTGAAATTACTCGAGATCTCCAGAGGAAGAGATCGTCAGCAGTAAATGCAGGCACAGGTTCGGGTTCGTTGCCGGGGAGATCAAAAGCCATTTTCCATAACTTGCCGGAAAATTTTATGTCAGACAAGTCTAGAGGGAGTTCTAGTGATACAGATGATGAATTCTGAGCTGAACTTATTCTTAAATGTATTCTAAGTTTTGGATTTTTTTGGTATAAACGAATGTACATAAGATTCTTAATCAAATCAGGAACATAAGATAAGAAGTAACAATAGGGCATTTTTTCATGTATTTAAATTGTTTGATAAAATTTCGTCATAGCATTAAGTCTATGATGTGCAAATCTAAGCATCATCCAAATGAATTACAAAAGCTT

Coding sequence (CDS)

ATGGATTCTCTATCGCACCTTATTCTCTTCCTCTTCTTCCTTTCTCTTTCCCCTCTTTCCTACTCCCAACTCAGCCCCCCTCAAAATATCGAAACTTACTACCCTTTCCCTCAACCGCCCTCGCCCTCCTCTTCTTCAGTTGACCATCCGCCATCGTCCACGTCCACCAAGACCATCGCCACCGCGGTGGCCGTCACTGCTGTCGGTGTTGCTCTGATTTCCACCTTCTTCTTCTTTTTAATACAGAGATATGTTATCGGCAGAAAGAGGAAAACAGAGGTCGTAAATTCAGGGACGGGTTCGGGTTCGGGTTCGGCAGTGCCACCGGCGGTTGCTCAGAGTGAATTTTCGCGGGTTGATGGGAATCTTAAAGGCTTTATTGTGGATGAAAATGGGTTGGATGTGATTTATTGGAAGAAACTTGAACAAAGGAAATCCAAAAATAGCTTTGATCGGGATGTTGAAGGTAATGTTAAGGAAAACCGAACAAAAAAGTCTGAGCCTGTTCAAGAAATTCCTCTGCTTCGAGGAAAATCTTCAACTTCGCATGTTAAAATTGCACCCGAAGATGAAGACGATAGTCGGATTACATCGCCACCGCCTCCTCCGCCGCCCCAGGTTAACCAACCTCCGCAATTTACTGCGATTTCGGTTCAGGCTGTTGGAAAATCACCAAGTAGTTCAAATCTTTCATCAACAGCACCAACGCAATCGACGGGAAATCAGGTACCTCCGAAACAATCTCCAATGGCCGTTCCGCCACCGCCCCCACCTATTCCAGCCAAGACCAATTCAAGACTGCCGCCGCCGCCACCTCCCATTCCCAAGACCAATTCAAGACCGCCGCCACCGCCGCCTCCTATTCAACCCAAGACCAACTCCGCAGGACCACCTCCTCCTCCTATACCGGCCAAGGCAAATCCGTCAGCACCACCGCCACCACCGCCCAAGGCCGGCGGTTCAAAATTACCCTTAAGGCCTGCACCTCCAAAAGAGAGTAACAAATCTTCTGCAGAGGCTTCTTCATCAGCTGACAATGGTCAGGTTAAAATGAAGCCCCTGCATTGGGATAAAGTGAACACTGCCAATGCCGATCATTCCATGGTTTGGGACAAAATGACCGCCGGTTCTTTCAAATTCGATGGGGATCTTATGGAAGCTCTCTTTGGATATGTCGCAACCAACCGGAAATCCCCCCGGAGTGAAGCTAGTTCTTCAGCAATCGCCGTTGGCCGGAACTCGGGGCCGTCGCAAACTTTCATTCTTGAACCGAAAAAATCTCAGAACATCGCCATTGTAATCAAGTCCTTAACAGTTCCTCGCAACGAAATCCTCGATGCACTCAACGAAGGTCAAGGCCTGGAAACAGAGGTTCTTGAAAAACTCACAAGAATTGCTCTGACTCAAGAAGAAATTTCCCAAATTCTTGCTTACAAAGGAGATCCCCAGAAGCTAGCTGATGCTGAATCTTTCCTTTATGATCTTCTCAAATCGGTTCCATCGGCCTTTACGCGCTTCAACGCCATGCTTTTCCGATTGAATTTTACGTCAGATATTCTCCATCACAAGGAATCTCTACAAACTCTGGAATCCGCTTGCAAGGAGCTTAGGACACGAGGATTGTTTATGAAACTTCTTGAAGCAATTCTCAAAGCTGGGAACCGGTTGAATGCAGGAACTGCAAGAGGAAATGCCAGAGCTTTCAATCTTACAGCTCTTCGAAAGCTCTCTGATGTTCGAAGCACTGATGGGAAAACCACTTTGCTTCATTTTGTAGTCCAGGAAGTGATCAGGGCAGAAGGGAAAAGGTGTGTTTTAAACAGGAACAAGAGCTTGAGCCGTAATAGCAGCCGTAGCAGCGACAACAGCTTTAGCAGCTTAGAAAACTCGGCTGCGAAGGAGGACAGAGTGAAGGAATACATGATGTTGGGATTGCCAGTGGTGGGAGGTCTAAGCTCCGAATTCTCTCATGTAAAGAAAGCGTCGGCGATCGACTACGAGAGCTTCGTCAAGGCAGGGACGTCCCTGACTAGTCGAACCGAAGAAATCCGGAAGCTCCTGACTCAAATGGGGAACAATGAAGGTGGGTTTGCGAAGGAAATGAGAGAATTTCTTGATGCAGCAGAGAATGAGCTGAAGATGGTAAGAGAAGCACAAACGAAGGTGATGGATTTAGTGATGAAGACAACAGAGTATTATCAGGCAGGAAGTTCTAAAGATAAAGAAACAAATCGGCTTCAATTGTTTATTATAATTAAAGATTTTCTAGAAATGGTAGATCGAGTTTGTGTTGAAATTACTCGAGATCTCCAGAGGAAGAGATCGTCAGCAGTAAATGCAGGCACAGGTTCGGGTTCGTTGCCGGGGAGATCAAAAGCCATTTTCCATAACTTGCCGGAAAATTTTATGTCAGACAAGTCTAGAGGGAGTTCTAGTGATACAGATGATGAATTCTGA

Protein sequence

MDSLSHLILFLFFLSLSPLSYSQLSPPQNIETYYPFPQPPSPSSSSVDHPPSSTSTKTIATAVAVTAVGVALISTFFFFLIQRYVIGRKRKTEVVNSGTGSGSGSAVPPAVAQSEFSRVDGNLKGFIVDENGLDVIYWKKLEQRKSKNSFDRDVEGNVKENRTKKSEPVQEIPLLRGKSSTSHVKIAPEDEDDSRITSPPPPPPPQVNQPPQFTAISVQAVGKSPSSSNLSSTAPTQSTGNQVPPKQSPMAVPPPPPPIPAKTNSRLPPPPPPIPKTNSRPPPPPPPIQPKTNSAGPPPPPIPAKANPSAPPPPPPKAGGSKLPLRPAPPKESNKSSAEASSSADNGQVKMKPLHWDKVNTANADHSMVWDKMTAGSFKFDGDLMEALFGYVATNRKSPRSEASSSAIAVGRNSGPSQTFILEPKKSQNIAIVIKSLTVPRNEILDALNEGQGLETEVLEKLTRIALTQEEISQILAYKGDPQKLADAESFLYDLLKSVPSAFTRFNAMLFRLNFTSDILHHKESLQTLESACKELRTRGLFMKLLEAILKAGNRLNAGTARGNARAFNLTALRKLSDVRSTDGKTTLLHFVVQEVIRAEGKRCVLNRNKSLSRNSSRSSDNSFSSLENSAAKEDRVKEYMMLGLPVVGGLSSEFSHVKKASAIDYESFVKAGTSLTSRTEEIRKLLTQMGNNEGGFAKEMREFLDAAENELKMVREAQTKVMDLVMKTTEYYQAGSSKDKETNRLQLFIIIKDFLEMVDRVCVEITRDLQRKRSSAVNAGTGSGSLPGRSKAIFHNLPENFMSDKSRGSSSDTDDEF
Homology
BLAST of Cucsat.G9521 vs. ExPASy Swiss-Prot
Match: O48682 (Formin-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=FH4 PE=1 SV=2)

HSP 1 Score: 582.8 bits (1501), Expect = 6.1e-165
Identity = 402/841 (47.80%), Postives = 529/841 (62.90%), Query Frame = 0

Query: 3   SLSHLILFLFFLSLSPLSYSQLSPPQNIETYYP----FPQPPSPSSSSVDHPPSSTSTK- 62
           +L  L L LFF +    S+SQ   P+NIET++P     P   SP  S   +P SS+S   
Sbjct: 17  TLVFLTLILFFPN---QSFSQSDSPRNIETFFPNDTITPPVQSPVLSPPQNPSSSSSDSD 76

Query: 63  --TIATAVAVTAVGVALISTFFFFLIQRYVIGRKRKTEVVNSGTGSGSGSAVPP----AV 122
              I  AV +TA    L++  FFFL+ +    R+R+  V   G  +     VPP    A+
Sbjct: 77  RGNILRAVLITAASTLLVAAVFFFLVHKC---RRRRNRV--GGVDNTLQPPVPPLAEAAL 136

Query: 123 AQSEFSRVDGNLKGFIVDENGLDVIYWKKLEQRKSKN---SFDRDV------EGNVKENR 182
           A+  F+R  GN+KG I+DENGLDV+YW+KL+Q +  N   SF +++      E NV  ++
Sbjct: 137 AREGFTRFGGNVKGLILDENGLDVLYWRKLQQSQRDNKGGSFRKEIIHGDDEEKNVIYSK 196

Query: 183 T-KKSEPVQEIPLLRGKSSTSHVKIAPEDEDDSRITSPPPPPPPQVNQPPQFTAISVQAV 242
           + KKS PV E PLLRG+SSTSH  I  ++  ++  T            PP     S + V
Sbjct: 197 SKKKSGPVTETPLLRGRSSTSHSVIHNDNYRNATTT-----------HPPHVKTDSFEFV 256

Query: 243 GKSPSSSNLSSTAPTQSTGNQVPPKQSPMAVPPPPPPIPAKTNSRLPPPPPPIPKTNSRP 302
              P+                 PP       PPPPPPIP K ++  PPPPPP  K N   
Sbjct: 257 KPDPT-----------------PP-------PPPPPPIPVKQSATPPPPPPPKLKNNGPS 316

Query: 303 PPPPPPIQPKTNSAGPPPPPIPAKANPSAPPPPPPKAGGSKLPLRPAPPKESNKSSAEAS 362
           PPPPPP++ KT           A  + SA   PPP   GS              SS E+S
Sbjct: 317 PPPPPPLK-KT-----------AALSSSASKKPPPAPRGS--------------SSGESS 376

Query: 363 SSADNGQVKMKPLHWDKVNTANADHSMVWDKMTAGSFKFDGDLMEALFGYVATNRKSPRS 422
               NGQVK+KPLHWDKVN  ++DHSMVWDK+  GSF FDGDLMEALFGYVA  +KSP  
Sbjct: 377 ----NGQVKLKPLHWDKVN-PDSDHSMVWDKIDRGSFSFDGDLMEALFGYVAVGKKSPDD 436

Query: 423 EASSSAIAVGRNSGPSQTFILEPKKSQNIAIVIKSLTVPRNEILDALNEGQGLETEVLEK 482
                      ++ P+Q FIL+P+KSQN AIV+KSL + R+E++++L EG     + LE+
Sbjct: 437 GGDKKP----SSASPAQIFILDPRKSQNTAIVLKSLGMTRDELVESLMEGHDFHPDTLER 496

Query: 483 LTRIALTQEEISQILAYKGDPQKLADAESFLYDLLKSVPSAFTRFNAMLFRLNFTSDILH 542
           L+RIA T+EE S IL + GD + LADAESFL+ LLK+VP AFTR NA+LFR N+  +I +
Sbjct: 497 LSRIAPTKEEQSAILQFDGDTKMLADAESFLFHLLKAVPCAFTRLNALLFRANYYPEISN 556

Query: 543 HKESLQTLESACKELRTRGLFMKLLEAILKAGNRLNAGTARGNARAFNLTALRKLSDVRS 602
           H ++LQTL+ AC ELR+RGLF+KLLEAILK+GNR+NAGTARG+A+AFNLTAL KLSDV+S
Sbjct: 557 HNKNLQTLDLACTELRSRGLFVKLLEAILKSGNRMNAGTARGDAQAFNLTALLKLSDVKS 616

Query: 603 TDGKTTLLHFVVQEVIRAEGKRCVLNR--NKSLSRNSSRSSDNSFSSLENSAAKEDRVKE 662
            DGKTTLL+FVV+EV+R+EGKRCVLNR  N+S SR+SS       SS+    +KE++ KE
Sbjct: 617 VDGKTTLLNFVVEEVVRSEGKRCVLNRRTNRSFSRSSS-------SSISEVISKEEQEKE 676

Query: 663 YMMLGLPVVGGLSSEFSHVKKASAIDYESFVKAGTSLTSRTEEIRKLLTQM-GNNEGG-- 722
           Y+ LGLPVVGGLSSEF++VKKA+A+DY++      +LTSR ++ R++L Q  G+N+ G  
Sbjct: 677 YLRLGLPVVGGLSSEFTNVKKAAAVDYDTVAATCLALTSRAKDARRVLAQSEGDNKEGVR 736

Query: 723 FAKEMREFLDAAENELKMVREAQTKVMDLVMKTTEYYQAGSSKDKETNRLQLFIIIKDFL 782
           F K+M EFLD+ E E+K+ +E + KV++LV +TTEYYQAG+ K K  N L LF+I++DFL
Sbjct: 737 FVKKMNEFLDSVEEEVKLAKEEEKKVLELVKRTTEYYQAGAVKGK--NPLHLFVIVRDFL 762

Query: 783 EMVDRVCVEITRDLQRKRSSAVNAGTGSGSLPGRSKAIFHNLPENFMSDKSRGSSSDTDD 818
            MVD+VCVEI R+LQR+ S         GS   R+   F  LP NFMSD+SR  S  +D 
Sbjct: 797 AMVDKVCVEIARNLQRRSS--------MGSTQQRNAVKFPVLPPNFMSDRSRSDSGGSDS 762

BLAST of Cucsat.G9521 vs. ExPASy Swiss-Prot
Match: O04532 (Formin-like protein 8 OS=Arabidopsis thaliana OX=3702 GN=FH8 PE=1 SV=1)

HSP 1 Score: 557.8 bits (1436), Expect = 2.1e-157
Identity = 381/841 (45.30%), Postives = 506/841 (60.17%), Query Frame = 0

Query: 7   LILFLFFLSLSPLSYSQLSPPQNIETYYPFPQ---------PPSPSSSSVDHPPSSTSTK 66
           LI F F + L   S SQ   PQNIET++P            PPS + S   +  SS+  K
Sbjct: 14  LIYFFFIVVLPFQSLSQFDSPQNIETFFPISSLSPVPPPLLPPSSNPSPPSNNSSSSDKK 73

Query: 67  TIATAVAVTAVGVALISTFFFFLIQRYVIGRKRKTEVVNSGTGSGSGSAVPP-------- 126
           TI  AV +TA    L++  FFF +QR +I R+R+  V      +      PP        
Sbjct: 74  TITKAVLITAASTLLVAGVFFFCLQRCIIARRRRDRVGPVRVENTLPPYPPPPMTSAAVT 133

Query: 127 --AVAQSEFSRVDGNLKGFIVDENGLDVIYWKKLE-QRKSKNSFDRDV----EGNVKE-- 186
              +A+  F+R  G +KG I+DENGLDV+YW+KL+ QR+   SF + +    E + KE  
Sbjct: 134 TTTLAREGFTRF-GGVKGLILDENGLDVLYWRKLQSQRERSGSFRKQIVTGEEEDEKEVI 193

Query: 187 --NRTKKSEPVQEIPLLRGKSSTSHVKIAPEDEDDSRITSPPPPPPPQVNQPPQFTAISV 246
                KK+EPV EIPLLRG+SSTSH  I  ED            PPPQV           
Sbjct: 194 YYKNKKKTEPVTEIPLLRGRSSTSHSVIHNEDHQ----------PPPQV----------- 253

Query: 247 QAVGKSPSSSNLSSTAPTQSTGNQVPPKQSPMAVPPPPPPIPAKTNSRLPPPPPPIPKTN 306
                                      KQS    PPPPP I  K ++  P PPPPI K +
Sbjct: 254 ---------------------------KQSEPTPPPPPPSIAVKQSAPTPSPPPPIKKGS 313

Query: 307 SRPPPPPPPIQPKTNSAGPPPPPIPAKANPSAPPPPPPKAGGSKLPLRPAPPKESNKSSA 366
           S  PPPPPP++            + A ++ ++ PPP P  G                   
Sbjct: 314 SPSPPPPPPVK-----------KVGALSSSASKPPPAPVRG------------------- 373

Query: 367 EASSSADNGQVKMKPLHWDKVNTANADHSMVWDKMTAGSFKFDGDLMEALFGYVATNRKS 426
            AS    + QVK+KPLHWDKVN  ++DHSMVWDK+  GSF FDGDLMEALFGYVA  +KS
Sbjct: 374 -ASGGETSKQVKLKPLHWDKVN-PDSDHSMVWDKIDRGSFSFDGDLMEALFGYVAVGKKS 433

Query: 427 PRSEASSSAIAVGRNSGPSQTFILEPKKSQNIAIVIKSLTVPRNEILDALNEGQGLETEV 486
           P            +N   +Q FIL+P+KSQN AIV+KSL + R E++++L EG     + 
Sbjct: 434 PEQGDE-------KNPKSTQIFILDPRKSQNTAIVLKSLGMTREELVESLIEGNDFVPDT 493

Query: 487 LEKLTRIALTQEEISQILAYKGDPQKLADAESFLYDLLKSVPSAFTRFNAMLFRLNFTSD 546
           LE+L RIA T+EE S IL + GD  KLADAE+FL+ LLKSVP+AFTR NA LFR N+  +
Sbjct: 494 LERLARIAPTKEEQSAILEFDGDTAKLADAETFLFHLLKSVPTAFTRLNAFLFRANYYPE 553

Query: 547 ILHHKESLQTLESACKELRTRGLFMKLLEAILKAGNRLNAGTARGNARAFNLTALRKLSD 606
           + HH + LQTL+ ACKELR+RGLF+KLLEAILKAGNR+NAGTARGNA+AFNLTAL KLSD
Sbjct: 554 MAHHSKCLQTLDLACKELRSRGLFVKLLEAILKAGNRMNAGTARGNAQAFNLTALLKLSD 613

Query: 607 VRSTDGKTTLLHFVVQEVIRAEGKRCVLN-RNKSLSRNSSRSSDNSFSSLENSAAKEDRV 666
           V+S DGKT+LL+FVV+EV+R+EGKRCV+N R+ SL+R+ S + +   SSL+   +KE++ 
Sbjct: 614 VKSVDGKTSLLNFVVEEVVRSEGKRCVMNRRSHSLTRSGSSNYNGGNSSLQ-VMSKEEQE 673

Query: 667 KEYMMLGLPVVGGLSSEFSHVKKASAIDYESFVKAGTSLTSRTEEIRKLLTQMGNNEGG- 726
           KEY+ LGLPVVGGLSSEFS+VKKA+ +DYE+ V   ++L  R ++ + ++ +  + EGG 
Sbjct: 674 KEYLKLGLPVVGGLSSEFSNVKKAACVDYETVVATCSALAVRAKDAKTVIGECEDGEGGR 733

Query: 727 FAKEMREFLDAAENELKMVREAQTKVMDLVMKTTEYYQAGSSKDKETNRLQLFIIIKDFL 786
           F K M  FLD+ E E+K+ +  + KVM+LV +TT+YYQAG +  K  N L LF+I++DFL
Sbjct: 734 FVKTMMTFLDSVEEEVKIAKGEERKVMELVKRTTDYYQAG-AVTKGKNPLHLFVIVRDFL 759

Query: 787 EMVDRVCVEITRDLQRKRSSAVNAGTGSGSLPGRSKAIFHNLPENFMSDKSRGSSSDTDD 818
            MVD+VC++I R++QR++      G+       R+   F  LP NFMSD++   S  +D 
Sbjct: 794 AMVDKVCLDIMRNMQRRK-----VGSPISPSSQRNAVKFPVLPPNFMSDRAWSDSGGSDS 759

BLAST of Cucsat.G9521 vs. ExPASy Swiss-Prot
Match: A3AB67 (Formin-like protein 16 OS=Oryza sativa subsp. japonica OX=39947 GN=FH16 PE=2 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 3.8e-135
Identity = 368/920 (40.00%), Postives = 504/920 (54.78%), Query Frame = 0

Query: 8   ILFLFFLSLSPLSYSQLSPPQNIETYY----------------PFPQPPSPSSSSVDHPP 67
           +L L  + ++PL+ +Q    QNI+T +                P P P +P+++    PP
Sbjct: 14  LLLLLLVGVAPLAAAQ---GQNIQTRFPSTRTPAFATPPPITSPSPSPGTPTATPSSSPP 73

Query: 68  SSTSTKT-IATAVAVTAVGVALISTFFFFLIQRYVIGRKRK-TEVVNSGT--GSGSGSAV 127
           SS+  ++ IA AV  TA+    +S   FFL  R+  G+KR+ TE   +G   G   G A+
Sbjct: 74  SSSGKRSDIAVAVVSTALSSFAVSGLAFFLFLRH--GKKRELTEAGGAGQHYGGAQGGAL 133

Query: 128 PPAVAQSEFSRVDGNLKGFIVDENGLDVIYWKKLEQR----------------------- 187
                + E  R     +G +VDENGLD IYW++ E+                        
Sbjct: 134 TGKRPEREPKR---PARGNMVDENGLDAIYWREFEKEGDGGRGRKPPASRRPPQPPPPRP 193

Query: 188 -KSKNSFDRDVEGNVKENRTKKSEPVQEIPLLRGK-----------------SSTSHVKI 247
            +++   D          R++K+   QE  + RG                   STS   +
Sbjct: 194 YRAERRQDAHESSAPSPPRSRKNRIDQEPLIPRGSLDSASAEFDESLYAPSAGSTSSFSV 253

Query: 248 APED-----EDDSRIT--------SPPPPPPPQVNQPPQFTAISVQAVGKSPSSSNLSST 307
           A  +          IT        SP P P P         ++ +    +SPS     + 
Sbjct: 254 AAAEAYARPPSTPAITAVSSVPRSSPSPAPAPAARPASPSPSLPLPPGRESPSRPQSIAA 313

Query: 308 APTQSTGNQVPPKQSPMAVPPPPPPIPAKTNSRLPPPPPPIPKTNSRPPPPPPPIQPKTN 367
           A   S     PP   P A  PPPPP P       PP  PP P     PPPPPPP  P   
Sbjct: 314 AAVASPAPPPPPPPKPAAAAPPPPPPPKAAPPPPPPKGPPPPPPAKGPPPPPPPKGPS-- 373

Query: 368 SAGPPPPPIPAKANPSAPPPPPPKAGGSKLPLRPAPPKESNKSSAEASSSADNGQVKMKP 427
               PPPP P       PPPPPPK G S+ P  P  P          + SAD  Q K+KP
Sbjct: 374 ----PPPPPPPGGKKGGPPPPPPKGGASRPPAAPGVP----------TGSADQ-QAKLKP 433

Query: 428 LHWDKVNTANADHSMVWDKMTAGSFKFDGDLMEALFGYVATNRK-----SPRSEASSSAI 487
           LHWDKVN A  DHSMVWD +T GSF  D  ++EALFG  A NRK     S  +   S++ 
Sbjct: 434 LHWDKVNVAATDHSMVWDNITGGSFNLDEGIIEALFGTAAVNRKTKPADSKDASGGSTSA 493

Query: 488 AVGRNSGPSQTFILEPKKSQNIAIVIKSLTVPRNEILDALNEGQ-GLETEVLEKLTRIAL 547
            +GR++ P Q F+LEP+KS NI+I+++SLTV R EI+DAL  G   L TEVLEKL+R+ +
Sbjct: 494 GLGRSNSPEQIFLLEPRKSHNISIILRSLTVGREEIIDALLNGHTELSTEVLEKLSRLNI 553

Query: 548 TQEEISQILAYKGDPQKLADAESFLYDLLKSVPSAFTRFNAMLFRLNFTSDILHHKESLQ 607
           ++EE + +L + G+P +LA AE FL  LL  VPS F R NA+LF+ N+ +++   K+SL+
Sbjct: 554 SKEEENTLLKFSGNPDRLAPAEFFLLRLLLDVPSPFARVNALLFKANYAAEVAQLKQSLR 613

Query: 608 TLESACKELRTRGLFMKLLEAILKAGNRLNAGTARGNARAFNLTALRKLSDVRSTDGKTT 667
           TLE A +ELRT+GLF KLLEA+LKAGNR+NAGTARGNA+AFNLTALRKLSDV+STDG TT
Sbjct: 614 TLEMASQELRTKGLFFKLLEAVLKAGNRMNAGTARGNAQAFNLTALRKLSDVKSTDGSTT 673

Query: 668 LLHFVVQEVIRAEGKRCVLNRNKSLSRNSS--RSSDN---SFSSLENSAAKEDRVKEYMM 727
           LLHFV++EV+R+EGKR  +NRN SL R+ S  +S+D    + SS     ++E+R  EY+ 
Sbjct: 674 LLHFVIEEVVRSEGKRLAINRNYSLRRSGSLAKSTDGGNPAASSTSQGPSREERQNEYLN 733

Query: 728 LGLPVVGGLSSEFSHVKKASAIDYESFVKAGTSLTSRTEEIRKLLTQMGNNEGGFAKEMR 787
           LGLP+VGGLS+EF++VKKA+ +DY++ V     L +R    +KLL   G++  GFA+ +R
Sbjct: 734 LGLPIVGGLSTEFANVKKAALVDYDTVVNECAILGNRLAGTKKLLETYGDD--GFARGLR 793

Query: 788 EFLDAAENELKMVREAQTKVMDLVMKTTEYYQAGSSKDKETNRLQLFIIIKDFLEMVDRV 817
            F+ AAE EL  ++  Q KV++LV +TTEYY  G++KDK  + LQLFII++DFL MVD+ 
Sbjct: 794 GFVKAAEQELNELKGNQEKVLELVQRTTEYYHTGATKDKNAHPLQLFIIVRDFLGMVDQA 853

BLAST of Cucsat.G9521 vs. ExPASy Swiss-Prot
Match: Q9XIE0 (Formin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=FH7 PE=2 SV=1)

HSP 1 Score: 464.9 bits (1195), Expect = 1.8e-129
Identity = 319/640 (49.84%), Postives = 406/640 (63.44%), Query Frame = 0

Query: 197 TSPPPPPPPQVNQPPQFTAI------SVQAVGKSPSSSNLSSTAPTQSTGNQVPPKQSPM 256
           TS P  P P    P Q+TA+      S Q V   P      + A + ST   + P Q   
Sbjct: 315 TSAPSVPLP----PGQYTAVNAPFSTSTQPVSLPPGQYMPGNAALSAST--PLTPGQFTT 374

Query: 257 AVPPPPPPIPAKTNSRLPPPPPPIPKTNSRPPPPPPPIQPKTNSAGPPPPPIPAKANPSA 316
           A  PP PP PA  N   PPPPPP    ++  PPPPPP  PK   A PPPPP P K     
Sbjct: 375 ANAPPAPPGPA--NQTSPPPPPP---PSAAAPPPPPP--PKKGPAAPPPPPPPGKKGAGP 434

Query: 317 PPPPP-PKAGGSKLPLRPAPPKESNKSSAEASSSADNGQVKMKPLHWDKVNTANADHSMV 376
           PPPPP  K G  K P  P  P +S ++S     + D  Q K+KPLHWDK+N  +A  SMV
Sbjct: 435 PPPPPMSKKGPPKPPGNPKGPTKSGETSLAVGKTEDPTQPKLKPLHWDKMN-PDASRSMV 494

Query: 377 WDKMTAGSFKFDGDLMEALFGYVATNRKSPRSEASSSAIAVGRNSGPSQTFILEPKKSQN 436
           W K+  GSF FDGDLMEALFGYVA  RK   S +      V  +   +QT+IL+P+KSQN
Sbjct: 495 WHKIDGGSFNFDGDLMEALFGYVA--RKPSESNSVPQNQTVSNSVPHNQTYILDPRKSQN 554

Query: 437 IAIVIKSLTVPRNEILDALNEGQGLETEVLEKLTRIALTQEEISQILAYKGDPQKLADAE 496
            AIV+KSL + + EI+D L EG   E++ LEKL  IA T EE ++I+ + G+P  LA A+
Sbjct: 555 KAIVLKSLGMTKEEIIDLLTEGHDAESDTLEKLAGIAPTPEEQTEIIDFDGEPMTLAYAD 614

Query: 497 SFLYDLLKSVPSAFTRFNAMLFRLNFTSDILHHKESLQTLESACKELRTRGLFMKLLEAI 556
           S L+ +LK+VPSAF RFN MLF++N+ S++   K SL TLESAC ELR RGLFMKLLEAI
Sbjct: 615 SLLFHILKAVPSAFNRFNVMLFKINYGSEVAQQKGSLLTLESACNELRARGLFMKLLEAI 674

Query: 557 LKAGNRLNAGTARGNARAFNLTALRKLSDVRSTDGKTTLLHFVVQEVIRAEGKRCVLNRN 616
           LKAGNR+NAGTARGNA+AFNLTALRKLSDV+S D KTTLLHFVV+EV+R+EGKR  +N+N
Sbjct: 675 LKAGNRMNAGTARGNAQAFNLTALRKLSDVKSVDAKTTLLHFVVEEVVRSEGKRAAMNKN 734

Query: 617 KSLSRNSSRSSDNSFSSLENSAAKEDRVKEYMMLGLPVVGGLSSEFSHVKKASAIDYESF 676
                    SSDN  S      ++E++  E++ +GLP++GGLSSEF++VKKA+ IDY+SF
Sbjct: 735 -------MMSSDNG-SGENADMSREEQEIEFIKMGLPIIGGLSSEFTNVKKAAGIDYDSF 794

Query: 677 VKAGTSLTSRTEEIRKLLTQMGNNEGGFAKEMREFLDAAENELKMVREAQTKVMDLVMKT 736
           V    +L +R +E ++LL Q    E G   ++R F ++AE ELK++ E Q ++M+LV KT
Sbjct: 795 VATTLALGTRVKETKRLLDQSKGKEDGCLTKLRSFFESAEEELKVITEEQLRIMELVKKT 854

Query: 737 TEYYQAGSSKDKETNRLQLFIIIKDFLEMVDRVCVEITRD---LQRKRSSAVNAGTGS-- 796
           T YYQAG+   KE N  QLF+II+DFL MVD  C EI R+    Q++R +   AG  S  
Sbjct: 855 TNYYQAGAL--KERNLFQLFVIIRDFLGMVDNACSEIARNQRKQQQQRPATTVAGASSSP 914

Query: 797 ------GSLPGRSKAIFHNLPENFMSDKSR-GSSSDTDDE 818
                  + P R+   F  LP NFMS+ SR  SSSD+D E
Sbjct: 915 AETPSVAAAPQRNAVRFPILPPNFMSESSRYSSSSDSDSE 928


HSP 2 Score: 4.6 bits (0), Expect = 6.8e+09
Identity = 68/283 (24.03%), Postives = 105/283 (37.10%), Query Frame = 0

Query: 102 GSGSAVPPA------VAQSEFSRVDGNLKGFIVDENGLDVIYWKKLEQRKSKNSFDRDVE 161
           GSGS +PP       +   +F R+DGNLK  IVD+ GLDVIYWKKL         + +  
Sbjct: 39  GSGSDLPPPPSPWGFLFPEDFVRIDGNLKAVIVDDEGLDVIYWKKL--------LELENS 98

Query: 162 GNVKENRTKKSEPVQEIPLLRGKSSTSHVKIAPEDEDDSRITSPPPPPPPQVNQPPQFTA 221
           G +++N           P  RG  S    +    D+DD+            + +   F  
Sbjct: 99  GKIRKNPK---------PRRRGDKSGDGFRRTGADQDDND-DGDDEVGDESIEEAFSFHV 158

Query: 222 ISVQAV------------------------GKSPSSSNLSSTAPTQSTGNQVPPKQSPMA 281
              Q+                         G+  +SS+ S + P+ S+ +   P ++  A
Sbjct: 159 KKSQSASSSGGEIRDQSNNGGGGGGGGGGGGRYYTSSSASPSRPSSSSASAASPSRTSYA 218

Query: 282 VPPPPP------PIPAKTNSRLPP----PPPPIPKTNSRPPPPPPPIQPKTNSAGPPPPP 341
                          +++  + P     P  P    +     PP P+ P   +AG    P
Sbjct: 219 TSAGSDYGGGGGGKQSQSKFQAPGGGSFPSSPSQIHSGGGRSPPLPLPPGQFTAGNASFP 278

Query: 342 IPAKANPSAPPPPPPKAGGSKLP-LRPAPPKESNKSSAEASSS 344
                + + PPP    AG +  P   P PP +    +A  SSS
Sbjct: 279 -----SSTQPPPGQYMAGNASFPSSTPPPPGQYMAGNAPFSSS 298

BLAST of Cucsat.G9521 vs. ExPASy Swiss-Prot
Match: Q10Q99 (Formin-like protein 8 OS=Oryza sativa subsp. japonica OX=39947 GN=FH8 PE=2 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 8.1e-77
Identity = 241/648 (37.19%), Postives = 357/648 (55.09%), Query Frame = 0

Query: 196 ITSPPPPPPPQVNQPPQFTAISVQAVGKSPSSSNLSSTAPTQSTGNQVPPKQSPMAVPPP 255
           I +PP PPP +  + P  T  S    G +P +  ++S +P         P Q   A PPP
Sbjct: 304 IAAPPAPPPARSRRTPPRTRFS---TGSTPDTKQVTSPSPR--------PVQPSNAPPPP 363

Query: 256 PPPIPAKTNSRLPPPPPPIPKTNSRPPPPPPPIQPKTNSAGPPPPPIPAKANPSAPPPPP 315
           PPP P       PPPPPP PK N+ P PPPPP         PPPP +P+  N   P  PP
Sbjct: 364 PPPPP-------PPPPPPPPKLNTAPKPPPPP---------PPPPSVPSNNNLPKPAEPP 423

Query: 316 PKAGGSKLPLRPAPPK----------------ESNKSSAEA---SSSADNG-----QVKM 375
                 +  L+P PP+                ++N S++     +++AD+G     + K+
Sbjct: 424 AVPTSRRRLLKPLPPEGPRIAMPMPITAATTVDNNGSTSMREGDNAAADDGGSGEPRPKL 483

Query: 376 KPLHWDKVNTANADHSMVWDKMTAGSFKFDGDLMEALFGYVATNRKSPRSEASSSAIAVG 435
           KPLHWDKV  A +D +MVWD++ + SF+ D D++EALF   +T    PR     +A   G
Sbjct: 484 KPLHWDKVR-ATSDRAMVWDQLKSSSFQLDEDMIEALFMNNSTPAAPPREVGRKAA---G 543

Query: 436 RNSGPSQTFILEPKKSQNIAIVIKSLTVPRNEILDALNEGQG--LETEVLEKLTRIALTQ 495
             S   +  +L+PKK+QNIAI++++L V R E+ DAL +G    L +E+LE L ++A T+
Sbjct: 544 VPSFRQEERVLDPKKAQNIAILLRALNVTREEVSDALLDGNAECLGSELLETLVKMAPTK 603

Query: 496 EEISQILAYKGDPQKLADAESFLYDLLKSVPSAFTRFNAMLFRLNFTSDILHHKESLQTL 555
           EE  ++  Y GD  KL  AE FL  +L  +P AF R +AML+R NF ++I + + S +TL
Sbjct: 604 EEELKLRDYSGDLSKLGSAERFLKAVL-DIPFAFKRVDAMLYRANFETEINYLRNSFETL 663

Query: 556 ESACKELRTRGLFMKLLEAILKAGNRLNAGTARGNARAFNLTALRKLSDVRSTDGKTTLL 615
           E+AC++LR   LF+KLLEA+L+ GNR+N GT RG A+AF L  L KL+DV+ TDGKTTLL
Sbjct: 664 EAACEDLRGSRLFLKLLEAVLRTGNRMNVGTNRGEAKAFKLDTLLKLADVKGTDGKTTLL 723

Query: 616 HFVVQEVIRAEGKRCVLNRNKSLSRNSSRSSDNSFSSLENSAAKEDRVKEYMMLGLPVVG 675
           HFVVQE+IR+E                   + +   S   S++K+DR       GL VV 
Sbjct: 724 HFVVQEIIRSE------------------DAKSEKESAMISSSKDDRKH-----GLKVVS 783

Query: 676 GLSSEFSHVKKASAIDYESFVKAGTSLTSRTEEIRKLLTQMGNNEGG--FAKEMREFLDA 735
           GLSSE  +VKKA+ +D++        L +  E+I+ +L        G  F   M++FL  
Sbjct: 784 GLSSELGNVKKAATMDFDVLHGYVNKLETGLEKIKSVLQLEKKCTQGQRFFMSMQDFLKE 843

Query: 736 AENELKMVREAQTKVMDLVMKTTEYYQAGSSKDKETNRLQLFIIIKDFLEMVDRVCVEIT 795
           AE E++ VR  + + +  V   TEY+   ++K+ E + L++F++++DFL  +D+VC E+ 
Sbjct: 844 AEREIERVRGEERRALGRVKDITEYFHGDTAKE-EAHPLRIFMVVRDFLSTLDQVCREVG 888

Query: 796 RDLQRKRSSAVNAGTGSGSLPGRSKAIFHNLPENFMSDKSRGSSSDTD 816
           R +Q+ R+         GS      +   +LP   +  + R ++SD D
Sbjct: 904 R-MQQDRTVI------GGSARSFRISATSSLPVLSLYGQRRENNSDDD 888

BLAST of Cucsat.G9521 vs. NCBI nr
Match: XP_004135469.1 (formin-like protein 4 [Cucumis sativus] >KGN51846.1 hypothetical protein Csa_008407 [Cucumis sativus])

HSP 1 Score: 1501 bits (3887), Expect = 0.0
Identity = 813/818 (99.39%), Postives = 815/818 (99.63%), Query Frame = 0

Query: 1   MDSLSHLILFLFFLSLSPLSYSQLSPPQNIETYYPFPQPPSPSSSSVDHPPSSTSTKTIA 60
           MDSLSHLILFLFFLSLSPLSYSQLSPPQNIETYYPFPQPPSPSSSSVDHPPSSTSTKTIA
Sbjct: 1   MDSLSHLILFLFFLSLSPLSYSQLSPPQNIETYYPFPQPPSPSSSSVDHPPSSTSTKTIA 60

Query: 61  TAVAVTAVGVALISTFFFFLIQRYVIGRKRKTEVVNSGTGSGSGSAVPPAVAQSEFSRVD 120
           TAVAVTAVGVALISTFFFFLIQRYVIGRKRKTEVVNSGTGSGSGSAVPPAVAQS+FSRVD
Sbjct: 61  TAVAVTAVGVALISTFFFFLIQRYVIGRKRKTEVVNSGTGSGSGSAVPPAVAQSDFSRVD 120

Query: 121 GNLKGFIVDENGLDVIYWKKLEQRKSKNSFDRDVEGNVKENRTKKSEPVQEIPLLRGKSS 180
           GNLKGFIVDENGLDVIYWKKLEQRKSKNSFDRDVEGNVKENRTKKSEPVQEIPLLRGKSS
Sbjct: 121 GNLKGFIVDENGLDVIYWKKLEQRKSKNSFDRDVEGNVKENRTKKSEPVQEIPLLRGKSS 180

Query: 181 TSHVKIAPEDEDDSRITSPPPPPPPQVNQPPQFTAISVQAVGKSPSSSNLSSTAPTQSTG 240
           TSHVKIAPEDEDDSRITSPPPPPPPQVNQPPQFTAISVQAVGKSPSSSNLSSTAPTQSTG
Sbjct: 181 TSHVKIAPEDEDDSRITSPPPPPPPQVNQPPQFTAISVQAVGKSPSSSNLSSTAPTQSTG 240

Query: 241 NQVPPKQSPMAVPPPPPPIPAKTNSRLPPPPPPIPKTNSRPPPPPPPIQPKTNSAGPPPP 300
           NQVPPKQSPMAVPPPPPPIPAKTNSRLPPPPPPIPKTNSRPPPPPPPIQPKTNSAGPPPP
Sbjct: 241 NQVPPKQSPMAVPPPPPPIPAKTNSRLPPPPPPIPKTNSRPPPPPPPIQPKTNSAGPPPP 300

Query: 301 PIPAKANPSAPPPPPPKAGGSKLPLRPAPPKESNKSSAEASSSADNGQVKMKPLHWDKVN 360
           PIPAKANPSAPPPPPPKAGGSKLPLRPAPPKESNKSSAEASSSADNGQVKMKPLHWDKVN
Sbjct: 301 PIPAKANPSAPPPPPPKAGGSKLPLRPAPPKESNKSSAEASSSADNGQVKMKPLHWDKVN 360

Query: 361 TANADHSMVWDKMTAGSFKFDGDLMEALFGYVATNRKSPRSEASSSAIAVGRNSGPSQTF 420
           TANADHSMVWDKMTAGSFKFDGDLMEALFGYVATNRKSPRSEASSSA AVGRNSGPSQTF
Sbjct: 361 TANADHSMVWDKMTAGSFKFDGDLMEALFGYVATNRKSPRSEASSSATAVGRNSGPSQTF 420

Query: 421 ILEPKKSQNIAIVIKSLTVPRNEILDALNEGQGLETEVLEKLTRIALTQEEISQILAYKG 480
           ILEPKKSQNIAIVIKSLTVPRN+ILDALNEGQGLETEVLEKLTRIALTQEEISQILAYKG
Sbjct: 421 ILEPKKSQNIAIVIKSLTVPRNDILDALNEGQGLETEVLEKLTRIALTQEEISQILAYKG 480

Query: 481 DPQKLADAESFLYDLLKSVPSAFTRFNAMLFRLNFTSDILHHKESLQTLESACKELRTRG 540
           D QKLADAESFLY LLKSVPSAFTRFNAMLFRLNFTSDILHHKESLQTLESACKELRTRG
Sbjct: 481 DHQKLADAESFLYHLLKSVPSAFTRFNAMLFRLNFTSDILHHKESLQTLESACKELRTRG 540

Query: 541 LFMKLLEAILKAGNRLNAGTARGNARAFNLTALRKLSDVRSTDGKTTLLHFVVQEVIRAE 600
           LFMKLLEAILKAGNRLNAGTARGNARAFNLTALRKLSDVRSTDGKTTLLHFVVQEVIRAE
Sbjct: 541 LFMKLLEAILKAGNRLNAGTARGNARAFNLTALRKLSDVRSTDGKTTLLHFVVQEVIRAE 600

Query: 601 GKRCVLNRNKSLSRNSSRSSDNSFSSLENSAAKEDRVKEYMMLGLPVVGGLSSEFSHVKK 660
           GKRCVLNRNKSLSRNSSRSSDNSFSSLENSAAKEDRVKEYMMLGLPVVGGLSSEFSHVKK
Sbjct: 601 GKRCVLNRNKSLSRNSSRSSDNSFSSLENSAAKEDRVKEYMMLGLPVVGGLSSEFSHVKK 660

Query: 661 ASAIDYESFVKAGTSLTSRTEEIRKLLTQMGNNEGGFAKEMREFLDAAENELKMVREAQT 720
           ASAIDYESFVKAGTSLTSRTEEIRKLLTQMGNNEGGFAKEMREFLDAAENELKMVREAQT
Sbjct: 661 ASAIDYESFVKAGTSLTSRTEEIRKLLTQMGNNEGGFAKEMREFLDAAENELKMVREAQT 720

Query: 721 KVMDLVMKTTEYYQAGSSKDKETNRLQLFIIIKDFLEMVDRVCVEITRDLQRKRSSAVNA 780
           KVMDLVMKTTEYYQAGSSKDKETNRLQLFIIIKDFLEMVDRVCVEITRDLQRKRSSAVNA
Sbjct: 721 KVMDLVMKTTEYYQAGSSKDKETNRLQLFIIIKDFLEMVDRVCVEITRDLQRKRSSAVNA 780

Query: 781 GTGSGSLPGRSKAIFHNLPENFMSDKSRGSSSDTDDEF 818
           GTGSGSLPGRSKAIFHNLPENFMSDKSRGSSSDTDDEF
Sbjct: 781 GTGSGSLPGRSKAIFHNLPENFMSDKSRGSSSDTDDEF 818

BLAST of Cucsat.G9521 vs. NCBI nr
Match: XP_008446271.1 (PREDICTED: formin-like protein 4 [Cucumis melo] >KAA0034348.1 formin-like protein 4 [Cucumis melo var. makuwa] >TYK15571.1 formin-like protein 4 [Cucumis melo var. makuwa])

HSP 1 Score: 1399 bits (3621), Expect = 0.0
Identity = 767/817 (93.88%), Postives = 782/817 (95.72%), Query Frame = 0

Query: 6   HLILFLFFLSLSPLSYSQLSPPQNIETYYP--FPQPPSPSSSSVDHPPSSTSTKTIATAV 65
           HL LFLFFLSLSPL Y QL+PPQNIET+YP   PQPPSPSSSS+DHPPSSTSTKTIATAV
Sbjct: 13  HLNLFLFFLSLSPLCYCQLNPPQNIETFYPPFIPQPPSPSSSSLDHPPSSTSTKTIATAV 72

Query: 66  AVTAVGVALISTFFFFLIQRYVIGRKRKTEVVNSGTGSGSGSAVPPAVAQSEFSRVDGNL 125
           AVTAVGVALIST FFFLIQ+YVIGRKRKTE VNSGTGSG    VPPAVAQSEFSRVDGNL
Sbjct: 73  AVTAVGVALISTLFFFLIQKYVIGRKRKTEEVNSGTGSGL--VVPPAVAQSEFSRVDGNL 132

Query: 126 KGFIVDENGLDVIYWKKLEQRKSKNSFDRDVEGNVKENRTKKSEPVQEIPLLRGKSSTSH 185
           KGFIVDENGLDVIYWK+LE+RKSKNSFDRDVEGNVKENR KKSEPVQEIPLLRGKSSTSH
Sbjct: 133 KGFIVDENGLDVIYWKRLEKRKSKNSFDRDVEGNVKENRNKKSEPVQEIPLLRGKSSTSH 192

Query: 186 VKIAPEDEDDSRITSPPPPPPPQVNQPPQFTAISVQAVGKSPSSSNLSSTAPTQSTGNQV 245
           VKIAPEDEDD+RITS  PPPPP +NQPPQF   SVQAVGKSPSSSNLSSTAP QST NQV
Sbjct: 193 VKIAPEDEDDTRITSSSPPPPPHINQPPQFAGNSVQAVGKSPSSSNLSSTAPPQSTDNQV 252

Query: 246 PPKQSPMAVPPPPPPIPAKTNSRLPPPPPPIPKTNSRPPPPPPPIQPKTNSAGPPPPPIP 305
           PP QSPMAVPPPPPPIPAKTNSRLPPPPPPIPKTNSRPPPPPPPIQ KTNSAGPPPPPIP
Sbjct: 253 PPTQSPMAVPPPPPPIPAKTNSRLPPPPPPIPKTNSRPPPPPPPIQTKTNSAGPPPPPIP 312

Query: 306 AKANPSAPPPPPPKAGGSKLPLRPAPPKESNKSSAEASSSADNGQVKMKPLHWDKVNTAN 365
           AKANPSAPPPPPPKAGGSKLPLRPAPPKESNKSS EASSSADNGQVKMKPLHWDKVNTAN
Sbjct: 313 AKANPSAPPPPPPKAGGSKLPLRPAPPKESNKSSGEASSSADNGQVKMKPLHWDKVNTAN 372

Query: 366 ADHSMVWDKMTAGSFKFDGDLMEALFGYVATNRKSPRSEASSSAIAVGRNSGPSQTFILE 425
           ADHSMVWDKM+AGSFKFDGDLMEALFGYVATNRKSPRSEASSSA AVGRNSGPSQTFILE
Sbjct: 373 ADHSMVWDKMSAGSFKFDGDLMEALFGYVATNRKSPRSEASSSANAVGRNSGPSQTFILE 432

Query: 426 PKKSQNIAIVIKSLTVPRNEILDALNEGQGLETEVLEKLTRIALTQEEISQILAYKGDPQ 485
           PKKSQNIAIVIKSLTVPRNEILDALNEG GLETEVLEKLTRIALTQEEISQILAY+GDPQ
Sbjct: 433 PKKSQNIAIVIKSLTVPRNEILDALNEGHGLETEVLEKLTRIALTQEEISQILAYRGDPQ 492

Query: 486 KLADAESFLYDLLKSVPSAFTRFNAMLFRLNFTSDILHHKESLQTLESACKELRTRGLFM 545
           KLADAESFLY LLKSVPSAFTRFNAMLFRLNFTS+ILH KESLQTLESACKELRTRGLFM
Sbjct: 493 KLADAESFLYHLLKSVPSAFTRFNAMLFRLNFTSEILHLKESLQTLESACKELRTRGLFM 552

Query: 546 KLLEAILKAGNRLNAGTARGNARAFNLTALRKLSDVRSTDGKTTLLHFVVQEVIRAEGKR 605
           KLLEAILKAGNRLNAGTARGNARAFNLTALRKLSDVRSTDGKTTLLHFVVQEVIRAEGKR
Sbjct: 553 KLLEAILKAGNRLNAGTARGNARAFNLTALRKLSDVRSTDGKTTLLHFVVQEVIRAEGKR 612

Query: 606 CVLNRNKSLSRNSSRSSDNSFSSLENSAAKEDRVKEYMMLGLPVVGGLSSEFSHVKKASA 665
           CVLNRNKSLSRNSSRSSDNSFSSLENS AKED+VKEYMMLGLPVVGGLSSEFSHVKKASA
Sbjct: 613 CVLNRNKSLSRNSSRSSDNSFSSLENSTAKEDKVKEYMMLGLPVVGGLSSEFSHVKKASA 672

Query: 666 IDYESFVKAGTSLTSRTEEIRKLLTQMGNNEGGFAKEMREFLDAAENELKMVREAQTKVM 725
           IDYESFVKAGTSLTSRTEEIRKLLTQMGNNEGGF KEMREFLDA+E+ELK VRE QTKVM
Sbjct: 673 IDYESFVKAGTSLTSRTEEIRKLLTQMGNNEGGFTKEMREFLDASEDELKKVREEQTKVM 732

Query: 726 DLVMKTTEYYQAGSSKDKETNRLQLFIIIKDFLEMVDRVCVEITRDLQRKRSSAVNAGT- 785
           DLVMKTTEYYQAGSSKDKETNRLQLFIIIKDFLEMVDRVCVEITRDLQRKRSSAVNAG+ 
Sbjct: 733 DLVMKTTEYYQAGSSKDKETNRLQLFIIIKDFLEMVDRVCVEITRDLQRKRSSAVNAGSS 792

Query: 786 -GSGSLPGRSKAIFHNLPENFMSDKSRGSSSDTDDEF 818
            GSGS PGRSKAIFHNLPENFMSDKSRGSSSDTDDEF
Sbjct: 793 SGSGSSPGRSKAIFHNLPENFMSDKSRGSSSDTDDEF 827

BLAST of Cucsat.G9521 vs. NCBI nr
Match: XP_038891749.1 (formin-like protein 4 [Benincasa hispida])

HSP 1 Score: 1276 bits (3302), Expect = 0.0
Identity = 720/839 (85.82%), Postives = 752/839 (89.63%), Query Frame = 0

Query: 1   MDSLSHLIL-------FLFFLSLSPLSYSQLSPPQNIETYYP---FPQPPSP--SSSSVD 60
           MD L HL L       FL FLSLSP+ Y QLSPPQNIET+YP    PQPPSP   SSS+D
Sbjct: 1   MDLLLHLHLQPWQLHLFLLFLSLSPVCYCQLSPPQNIETFYPSPVIPQPPSPISPSSSLD 60

Query: 61  HPPSSTSTKTIATAVAVTAVGVALISTFFFFLIQRYVIGRKRKTEVVNSGTGSGSGSAVP 120
           HPPSSTSTKTIATAVAVTAVGVALIST FFFLIQRYVIGRKRKTE  NSGTG  +G    
Sbjct: 61  HPPSSTSTKTIATAVAVTAVGVALISTLFFFLIQRYVIGRKRKTEEANSGTGP-AGLNSQ 120

Query: 121 PAVAQSEFSRVDGNLKGFIVDENGLDVIYWKKLEQRKSKNSFDR-DVEGNVKENRTKKSE 180
           PAVAQSEFSRVDGNLKGFIVDENGLDVIYWK+LE++KSKNSF R D EGNVKENR+KKSE
Sbjct: 121 PAVAQSEFSRVDGNLKGFIVDENGLDVIYWKRLERKKSKNSFHRSDGEGNVKENRSKKSE 180

Query: 181 PVQEIPLLRGKSSTSHVKIAPEDEDDSRITSPPPPPPPQVNQPPQFTAISVQAVGKSPSS 240
           PVQEIPLLRGKSSTSHVKIAPEDE+ SRITSPPPPPPPQ+N PP F   SVQ+VGK  SS
Sbjct: 181 PVQEIPLLRGKSSTSHVKIAPEDEESSRITSPPPPPPPQINNPPPFIENSVQSVGKPSSS 240

Query: 241 SNLSSTAPTQSTGNQVPPKQSPMAVP-------PPPPPIPAKTNSRLPPPPPPIP-KTNS 300
           SNLSS AP Q    QVPP QS MAVP       PPPP IPAKTNSRLPPPPPPIP KTNS
Sbjct: 241 SNLSSAAPPQPAEIQVPPSQSLMAVPKNKSSVPPPPPSIPAKTNSRLPPPPPPIPAKTNS 300

Query: 301 RPPPPPPPIQPKTNSAGPPPPPIPAKANPSAPPPPPPKAGGSKLPLRPAPPKESNKSSAE 360
           RPPPPPPPIQ KTNSAGPPPPPIPAKANP+APP PPPK GGSKLP RPAP KE NKSS E
Sbjct: 301 RPPPPPPPIQSKTNSAGPPPPPIPAKANPAAPPLPPPKPGGSKLPSRPAPAKEGNKSSGE 360

Query: 361 ASSSADNGQVKMKPLHWDKVNTANADHSMVWDKMTAGSFKFDGDLMEALFGYVATNRKSP 420
           ASSSADNGQVKMKPLHWDKVNTANADHSMVWDKM+AGSFKFDGDLMEALFGYVATNRKSP
Sbjct: 361 ASSSADNGQVKMKPLHWDKVNTANADHSMVWDKMSAGSFKFDGDLMEALFGYVATNRKSP 420

Query: 421 RSEASSSAIAVGRNSGPSQTFILEPKKSQNIAIVIKSLTVPRNEILDALNEGQGLETEVL 480
           RSEASSSA AVGRNSGPSQTFILEPKKSQNIAIVIKSLT+PRNEILDALNEGQGLETE+L
Sbjct: 421 RSEASSSANAVGRNSGPSQTFILEPKKSQNIAIVIKSLTIPRNEILDALNEGQGLETEIL 480

Query: 481 EKLTRIALTQEEISQILAYKGDPQKLADAESFLYDLLKSVPSAFTRFNAMLFRLNFTSDI 540
           EKLTRIA TQEEISQILAYKGDPQKLADAESFLY LLKSVPSAFTRFNAMLFRLNFTS+I
Sbjct: 481 EKLTRIAPTQEEISQILAYKGDPQKLADAESFLYHLLKSVPSAFTRFNAMLFRLNFTSEI 540

Query: 541 LHHKESLQTLESACKELRTRGLFMKLLEAILKAGNRLNAGTARGNARAFNLTALRKLSDV 600
           LH KES+QTLESACKELRTRGLF+KLLEAILKAGNRLNAGTARGNARAFNLTALRKLSDV
Sbjct: 541 LHLKESIQTLESACKELRTRGLFLKLLEAILKAGNRLNAGTARGNARAFNLTALRKLSDV 600

Query: 601 RSTDGKTTLLHFVVQEVIRAEGKRCVLNRNKSLSRNSSRSSDNSFSSLENSAAKEDRVKE 660
           RSTDGKTTLLHFVVQEV+RAEGK+C+LNR KSLSRNSS SSDNSFSS ENS AKEDRVKE
Sbjct: 601 RSTDGKTTLLHFVVQEVVRAEGKKCILNRKKSLSRNSSHSSDNSFSSSENSTAKEDRVKE 660

Query: 661 YMMLGLPVVGGLSSEFSHVKKASAIDYESFVKAGTSLTSRTEEIRKLLTQMGNNEGGFAK 720
           YMMLGLPVVGGLS+EFS+VKKA+AIDYESFVKA  SLTSRT EI++L TQ+GNNEGGFAK
Sbjct: 661 YMMLGLPVVGGLSAEFSNVKKAAAIDYESFVKAWPSLTSRTAEIQQLSTQIGNNEGGFAK 720

Query: 721 EMREFLDAAENELKMVREAQTKVMDLVMKTTEYYQAGSSKDKETNRLQLFIIIKDFLEMV 780
           EMR FL+AAE ELK V+E QTKVM+LVMKTTEYYQA SSKDKE NRLQLFII+KDFLEMV
Sbjct: 721 EMRVFLEAAERELKGVKEEQTKVMELVMKTTEYYQAVSSKDKEANRLQLFIIVKDFLEMV 780

Query: 781 DRVCVEITRDLQRKRSSAVNAGTGSGSLPGRSKAIFHNLPENFMSDKSRGSSSDTDDEF 818
           DRVCVEITRDLQR+RSS VN G GSGS P RSKAIFHNLPENFMSDKSRGSSSDTDDEF
Sbjct: 781 DRVCVEITRDLQRRRSSGVNMGLGSGSSPVRSKAIFHNLPENFMSDKSRGSSSDTDDEF 838

BLAST of Cucsat.G9521 vs. NCBI nr
Match: XP_022965655.1 (formin-like protein 4 [Cucurbita maxima])

HSP 1 Score: 1139 bits (2946), Expect = 0.0
Identity = 646/826 (78.21%), Postives = 711/826 (86.08%), Query Frame = 0

Query: 9   LFLFFLSLSPLSYSQLSPPQNIETYYPFPQPPSPSSSSVDHPP-------SSTSTKTIAT 68
           L L FLS+SP+SY Q +PPQNIET+YP   PP       DHPP       SS++T+TI T
Sbjct: 18  LLLLFLSVSPVSYCQDTPPQNIETFYPILPPPP---FLPDHPPPPLPPPSSSSTTRTIVT 77

Query: 69  AVAVTAVGVALISTFFFFLIQRYVIGRKRKTEVVNSGTGSGSGSAVPPAVAQSEFSRVDG 128
           AVA+TAVG+ALIST FFFLIQRY++ RK+KTE VNSG G G  S   PAVA++EF+RVDG
Sbjct: 78  AVAITAVGMALISTVFFFLIQRYLVRRKQKTEEVNSGPGQGPVSQ--PAVARNEFARVDG 137

Query: 129 NLKGFIVDENGLDVIYWKKLEQRKSKNSFDR-DVEGNVKENRTKKSEPVQEIPLLRGKSS 188
           NLKGFIVDE+GLDVIYWK+LE+RKSKNSFDR D EGNV+ NR+KKSEPVQEIPLLRGKSS
Sbjct: 138 NLKGFIVDEDGLDVIYWKRLERRKSKNSFDRGDGEGNVQANRSKKSEPVQEIPLLRGKSS 197

Query: 189 TSHVKIAPEDEDDSRITSPPPPPPPQVNQPPQFTAISVQAVGKSPSSSNLSSTAPTQSTG 248
           +SHVKI+PEDE+  R+T PPPPPPP +N PP FT  SVQ VGK  SSS LSS+AP Q T 
Sbjct: 198 SSHVKISPEDEEPRRVTPPPPPPPPHINNPPPFTGKSVQEVGKPSSSSILSSSAPPQPTA 257

Query: 249 NQVPPKQSPMAVP-------PPPPPIPAKTNSRLPPPPPPIP-KTNSRPPPPPPPIQPKT 308
             VPP QS MAVP       PPPPPIPAKT SR PPPPPPI  KTNSRPPPPPP I  KT
Sbjct: 258 IPVPPSQSLMAVPNNKCSVPPPPPPIPAKTVSRPPPPPPPIATKTNSRPPPPPP-IPAKT 317

Query: 309 NSAGPPPPPIPAKANPSAPPPPPPKAGGSKLPLRPAPPKESNKSSAEASSSADNGQVKMK 368
           NSA PPPPPI AKANP+APPPP  KAG SKLPLRPAP KE  KSS E+S+SADNGQVKMK
Sbjct: 318 NSAAPPPPPIVAKANPAAPPPP--KAGSSKLPLRPAPQKE-GKSSGESSTSADNGQVKMK 377

Query: 369 PLHWDKVNTANADHSMVWDKMTAGSFKFDGDLMEALFGYVATNRKSPRSEASSSAIAVGR 428
           PLHWDKVNTANADHSMVWDKM AGSFKFDGDLMEALFGYVATNRKSPRSEA+SS I  GR
Sbjct: 378 PLHWDKVNTANADHSMVWDKMQAGSFKFDGDLMEALFGYVATNRKSPRSEANSSVIPTGR 437

Query: 429 NSGPSQTFILEPKKSQNIAIVIKSLTVPRNEILDALNEGQGLETEVLEKLTRIALTQEEI 488
           NSGPSQTFILEPKKSQNIAIV+KSLT+PRNEILDALNEGQGLET++LEKLTRI LTQEEI
Sbjct: 438 NSGPSQTFILEPKKSQNIAIVVKSLTIPRNEILDALNEGQGLETDILEKLTRITLTQEEI 497

Query: 489 SQILAYKGDPQKLADAESFLYDLLKSVPSAFTRFNAMLFRLNFTSDILHHKESLQTLESA 548
           SQILAYKGDPQKLADAE+FLY LLKSVPSAF RFNAMLFRL F SDI H KESL+ LESA
Sbjct: 498 SQILAYKGDPQKLADAETFLYHLLKSVPSAFARFNAMLFRLTFGSDIHHLKESLEILESA 557

Query: 549 CKELRTRGLFMKLLEAILKAGNRLNAGTARGNARAFNLTALRKLSDVRSTDGKTTLLHFV 608
           CKELRTRGLF+KLLEA+LKAGNRLNAGTARGNARAFNL+ALRKLSDVRSTDGKTTLLHFV
Sbjct: 558 CKELRTRGLFLKLLEAVLKAGNRLNAGTARGNARAFNLSALRKLSDVRSTDGKTTLLHFV 617

Query: 609 VQEVIRAEGKRCVLNRNKSLSRNSSRSSDNSFSSLENSAAKEDRVKEYMMLGLPVVGGLS 668
           VQEVIRAEGKRCVLNRNKSLSRN+S +SD+S S+ +NS++KEDRV EYMMLGLPVVGGLS
Sbjct: 618 VQEVIRAEGKRCVLNRNKSLSRNTSHASDSSVSTSDNSSSKEDRVNEYMMLGLPVVGGLS 677

Query: 669 SEFSHVKKASAIDYESFVKAGTSLTSRTEEIRKLLTQMGNNEGGFAKEMREFLDAAENEL 728
           +EFS+VKKA+ IDYESF   GTSLTSRT EIR+L+ Q+GNN GGF KEMR FL+AAE+EL
Sbjct: 678 AEFSNVKKAATIDYESFANTGTSLTSRTAEIRQLMAQIGNNGGGFGKEMRGFLEAAESEL 737

Query: 729 KMVREAQTKVMDLVMKTTEYYQAGSSKDKETNRLQLFIIIKDFLEMVDRVCVEITRDLQR 788
           K+VRE QTKVM+LVMKTTEYYQAGSS+DKE+NRLQ F I+KDFLEMVDRVCVEI+R+LQ+
Sbjct: 738 KVVREEQTKVMELVMKTTEYYQAGSSRDKESNRLQFFTIVKDFLEMVDRVCVEISRNLQK 797

Query: 789 KRSSAVNAGTGSGSLPGRSKAIFHNLPENFMSDKSRGSSSDTDDEF 818
           +RSS VN G    S P RSKAIF NLP NFMSDKSRGSSSD+D+EF
Sbjct: 798 RRSSTVNVG----SSPVRSKAIFPNLPANFMSDKSRGSSSDSDNEF 830

BLAST of Cucsat.G9521 vs. NCBI nr
Match: KAG6600142.1 (Formin-like protein 8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1137 bits (2942), Expect = 0.0
Identity = 649/824 (78.76%), Postives = 709/824 (86.04%), Query Frame = 0

Query: 9   LFLFFLSLSPLSYSQLSPPQNIETYYPFPQPPS-----PSSSSVDHPPSSTSTKTIATAV 68
           L L FLS+SP+SY Q +PPQNIET+YP   PP      P S S    PSS ST+TI TAV
Sbjct: 16  LLLLFLSVSPVSYCQDTPPQNIETFYPILPPPPFLPVHPPSPSPSSSPSS-STRTIVTAV 75

Query: 69  AVTAVGVALISTFFFFLIQRYVIGRKRKTEVVNSGTGSGSGSAVPPAVAQSEFSRVDGNL 128
           A+TAVG+ALIST FFFLIQRY+I RKRKTE VNSG G G  S   PAVA+SEF+RVDGNL
Sbjct: 76  AITAVGMALISTVFFFLIQRYLIRRKRKTEEVNSGPGQGPVSQ--PAVARSEFARVDGNL 135

Query: 129 KGFIVDENGLDVIYWKKLEQRKSKNSFDR-DVEGNVKENRTKKSEPVQEIPLLRGKSSTS 188
           KGFIVDE+GLDVIYWK+LE+RKSKNSFDR D EGNV+ NR+KKSEPVQEIPLLRGKSS+S
Sbjct: 136 KGFIVDEDGLDVIYWKRLERRKSKNSFDRGDGEGNVQANRSKKSEPVQEIPLLRGKSSSS 195

Query: 189 HVKIAPEDEDDSRITSPPPPPPPQVNQPPQFTAISVQAVGKSPSSSNLSSTAPTQSTGNQ 248
           HVKI+PEDE+  R+T PPPPPP  +N PP FT  SVQ VGK  SSS LSS AP Q     
Sbjct: 196 HVKISPEDEEPRRVTPPPPPPPSHINNPPPFTGKSVQEVGKPSSSSILSSPAPPQPAAIP 255

Query: 249 VPPKQSPMAVP-------PPPPPIPAKTNSRLPPPPPPIP-KTNSRPPPPPPPIQPKTNS 308
           VPP QS MAVP       PPPPPIPAKT SR PPPPPPI  KTNSRPPPPPP I  KTNS
Sbjct: 256 VPPSQSLMAVPNNKSSVPPPPPPIPAKTVSRPPPPPPPIATKTNSRPPPPPP-IPAKTNS 315

Query: 309 AGPPPPPIPAKANPSAPPPPPPKAGGSKLPLRPAPPKESNKSSAEASSSADNGQVKMKPL 368
           A PPPPPI AKANP+APPPP  KAG SKLPLRPAP KE  KSS E+S+SADNGQVKMKPL
Sbjct: 316 AAPPPPPIVAKANPAAPPPP--KAGSSKLPLRPAPQKE-GKSSGESSTSADNGQVKMKPL 375

Query: 369 HWDKVNTANADHSMVWDKMTAGSFKFDGDLMEALFGYVATNRKSPRSEASSSAIAVGRNS 428
           HWDKVNTANADHSMVWDKM AGSFKFDGDLMEALFGYVATNRKSPRSEA+SSAI  GRNS
Sbjct: 376 HWDKVNTANADHSMVWDKMQAGSFKFDGDLMEALFGYVATNRKSPRSEANSSAIPTGRNS 435

Query: 429 GPSQTFILEPKKSQNIAIVIKSLTVPRNEILDALNEGQGLETEVLEKLTRIALTQEEISQ 488
           GPSQTFILEPKKSQNIAIV+KSLT+PRNEILDALNEGQGLET++LEKLTRI LTQEEISQ
Sbjct: 436 GPSQTFILEPKKSQNIAIVVKSLTIPRNEILDALNEGQGLETDILEKLTRITLTQEEISQ 495

Query: 489 ILAYKGDPQKLADAESFLYDLLKSVPSAFTRFNAMLFRLNFTSDILHHKESLQTLESACK 548
           ILAYKGDPQKLADAE+FLY LLK+VPSAF RFNAMLFRL F SDI H KESL+ LESACK
Sbjct: 496 ILAYKGDPQKLADAETFLYHLLKAVPSAFARFNAMLFRLTFGSDIHHLKESLEILESACK 555

Query: 549 ELRTRGLFMKLLEAILKAGNRLNAGTARGNARAFNLTALRKLSDVRSTDGKTTLLHFVVQ 608
           ELRTRGLF+KLLEA+LKAGNRLNAGTARGNARAFNL+ALRKLSDVRSTDGKTTLLHFVVQ
Sbjct: 556 ELRTRGLFLKLLEAVLKAGNRLNAGTARGNARAFNLSALRKLSDVRSTDGKTTLLHFVVQ 615

Query: 609 EVIRAEGKRCVLNRNKSLSRNSSRSSDNSFSSLENSAAKEDRVKEYMMLGLPVVGGLSSE 668
           EVIRAEGKRCVLNRNKSLSRN+S +SD+S S+ +NS++KEDRV EYMMLGLPVVGGLS+E
Sbjct: 616 EVIRAEGKRCVLNRNKSLSRNTSHASDSSVSTSDNSSSKEDRVNEYMMLGLPVVGGLSAE 675

Query: 669 FSHVKKASAIDYESFVKAGTSLTSRTEEIRKLLTQMGNNEGGFAKEMREFLDAAENELKM 728
           FS+VKKA+ IDYESF   GTSLTSRT EIR+L+ Q+GNN GGF KEMR FL+AAE+ELK+
Sbjct: 676 FSNVKKAATIDYESFANTGTSLTSRTAEIRQLMAQIGNNGGGFGKEMRGFLEAAESELKV 735

Query: 729 VREAQTKVMDLVMKTTEYYQAGSSKDKETNRLQLFIIIKDFLEMVDRVCVEITRDLQRKR 788
           VRE QTKVM+LVMKTTEYYQAGSS+DKE+NRLQ FII+KDFLEMVDRVCVEI+R+LQ++R
Sbjct: 736 VREEQTKVMELVMKTTEYYQAGSSRDKESNRLQFFIIVKDFLEMVDRVCVEISRNLQKRR 795

Query: 789 SSAVNAGTGSGSLPGRSKAIFHNLPENFMSDKSRGSSSDTDDEF 818
           SS VN G    S P RSKAIF NLP NFMSDKSRGSSSD+D+EF
Sbjct: 796 SSTVNVG----SSPVRSKAIFPNLPANFMSDKSRGSSSDSDNEF 828

BLAST of Cucsat.G9521 vs. ExPASy TrEMBL
Match: A0A0A0KVR0 (Formin-like protein OS=Cucumis sativus OX=3659 GN=Csa_5G603920 PE=3 SV=1)

HSP 1 Score: 1501 bits (3887), Expect = 0.0
Identity = 813/818 (99.39%), Postives = 815/818 (99.63%), Query Frame = 0

Query: 1   MDSLSHLILFLFFLSLSPLSYSQLSPPQNIETYYPFPQPPSPSSSSVDHPPSSTSTKTIA 60
           MDSLSHLILFLFFLSLSPLSYSQLSPPQNIETYYPFPQPPSPSSSSVDHPPSSTSTKTIA
Sbjct: 1   MDSLSHLILFLFFLSLSPLSYSQLSPPQNIETYYPFPQPPSPSSSSVDHPPSSTSTKTIA 60

Query: 61  TAVAVTAVGVALISTFFFFLIQRYVIGRKRKTEVVNSGTGSGSGSAVPPAVAQSEFSRVD 120
           TAVAVTAVGVALISTFFFFLIQRYVIGRKRKTEVVNSGTGSGSGSAVPPAVAQS+FSRVD
Sbjct: 61  TAVAVTAVGVALISTFFFFLIQRYVIGRKRKTEVVNSGTGSGSGSAVPPAVAQSDFSRVD 120

Query: 121 GNLKGFIVDENGLDVIYWKKLEQRKSKNSFDRDVEGNVKENRTKKSEPVQEIPLLRGKSS 180
           GNLKGFIVDENGLDVIYWKKLEQRKSKNSFDRDVEGNVKENRTKKSEPVQEIPLLRGKSS
Sbjct: 121 GNLKGFIVDENGLDVIYWKKLEQRKSKNSFDRDVEGNVKENRTKKSEPVQEIPLLRGKSS 180

Query: 181 TSHVKIAPEDEDDSRITSPPPPPPPQVNQPPQFTAISVQAVGKSPSSSNLSSTAPTQSTG 240
           TSHVKIAPEDEDDSRITSPPPPPPPQVNQPPQFTAISVQAVGKSPSSSNLSSTAPTQSTG
Sbjct: 181 TSHVKIAPEDEDDSRITSPPPPPPPQVNQPPQFTAISVQAVGKSPSSSNLSSTAPTQSTG 240

Query: 241 NQVPPKQSPMAVPPPPPPIPAKTNSRLPPPPPPIPKTNSRPPPPPPPIQPKTNSAGPPPP 300
           NQVPPKQSPMAVPPPPPPIPAKTNSRLPPPPPPIPKTNSRPPPPPPPIQPKTNSAGPPPP
Sbjct: 241 NQVPPKQSPMAVPPPPPPIPAKTNSRLPPPPPPIPKTNSRPPPPPPPIQPKTNSAGPPPP 300

Query: 301 PIPAKANPSAPPPPPPKAGGSKLPLRPAPPKESNKSSAEASSSADNGQVKMKPLHWDKVN 360
           PIPAKANPSAPPPPPPKAGGSKLPLRPAPPKESNKSSAEASSSADNGQVKMKPLHWDKVN
Sbjct: 301 PIPAKANPSAPPPPPPKAGGSKLPLRPAPPKESNKSSAEASSSADNGQVKMKPLHWDKVN 360

Query: 361 TANADHSMVWDKMTAGSFKFDGDLMEALFGYVATNRKSPRSEASSSAIAVGRNSGPSQTF 420
           TANADHSMVWDKMTAGSFKFDGDLMEALFGYVATNRKSPRSEASSSA AVGRNSGPSQTF
Sbjct: 361 TANADHSMVWDKMTAGSFKFDGDLMEALFGYVATNRKSPRSEASSSATAVGRNSGPSQTF 420

Query: 421 ILEPKKSQNIAIVIKSLTVPRNEILDALNEGQGLETEVLEKLTRIALTQEEISQILAYKG 480
           ILEPKKSQNIAIVIKSLTVPRN+ILDALNEGQGLETEVLEKLTRIALTQEEISQILAYKG
Sbjct: 421 ILEPKKSQNIAIVIKSLTVPRNDILDALNEGQGLETEVLEKLTRIALTQEEISQILAYKG 480

Query: 481 DPQKLADAESFLYDLLKSVPSAFTRFNAMLFRLNFTSDILHHKESLQTLESACKELRTRG 540
           D QKLADAESFLY LLKSVPSAFTRFNAMLFRLNFTSDILHHKESLQTLESACKELRTRG
Sbjct: 481 DHQKLADAESFLYHLLKSVPSAFTRFNAMLFRLNFTSDILHHKESLQTLESACKELRTRG 540

Query: 541 LFMKLLEAILKAGNRLNAGTARGNARAFNLTALRKLSDVRSTDGKTTLLHFVVQEVIRAE 600
           LFMKLLEAILKAGNRLNAGTARGNARAFNLTALRKLSDVRSTDGKTTLLHFVVQEVIRAE
Sbjct: 541 LFMKLLEAILKAGNRLNAGTARGNARAFNLTALRKLSDVRSTDGKTTLLHFVVQEVIRAE 600

Query: 601 GKRCVLNRNKSLSRNSSRSSDNSFSSLENSAAKEDRVKEYMMLGLPVVGGLSSEFSHVKK 660
           GKRCVLNRNKSLSRNSSRSSDNSFSSLENSAAKEDRVKEYMMLGLPVVGGLSSEFSHVKK
Sbjct: 601 GKRCVLNRNKSLSRNSSRSSDNSFSSLENSAAKEDRVKEYMMLGLPVVGGLSSEFSHVKK 660

Query: 661 ASAIDYESFVKAGTSLTSRTEEIRKLLTQMGNNEGGFAKEMREFLDAAENELKMVREAQT 720
           ASAIDYESFVKAGTSLTSRTEEIRKLLTQMGNNEGGFAKEMREFLDAAENELKMVREAQT
Sbjct: 661 ASAIDYESFVKAGTSLTSRTEEIRKLLTQMGNNEGGFAKEMREFLDAAENELKMVREAQT 720

Query: 721 KVMDLVMKTTEYYQAGSSKDKETNRLQLFIIIKDFLEMVDRVCVEITRDLQRKRSSAVNA 780
           KVMDLVMKTTEYYQAGSSKDKETNRLQLFIIIKDFLEMVDRVCVEITRDLQRKRSSAVNA
Sbjct: 721 KVMDLVMKTTEYYQAGSSKDKETNRLQLFIIIKDFLEMVDRVCVEITRDLQRKRSSAVNA 780

Query: 781 GTGSGSLPGRSKAIFHNLPENFMSDKSRGSSSDTDDEF 818
           GTGSGSLPGRSKAIFHNLPENFMSDKSRGSSSDTDDEF
Sbjct: 781 GTGSGSLPGRSKAIFHNLPENFMSDKSRGSSSDTDDEF 818

BLAST of Cucsat.G9521 vs. ExPASy TrEMBL
Match: A0A5D3CUJ4 (Formin-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G00650 PE=3 SV=1)

HSP 1 Score: 1399 bits (3621), Expect = 0.0
Identity = 767/817 (93.88%), Postives = 782/817 (95.72%), Query Frame = 0

Query: 6   HLILFLFFLSLSPLSYSQLSPPQNIETYYP--FPQPPSPSSSSVDHPPSSTSTKTIATAV 65
           HL LFLFFLSLSPL Y QL+PPQNIET+YP   PQPPSPSSSS+DHPPSSTSTKTIATAV
Sbjct: 13  HLNLFLFFLSLSPLCYCQLNPPQNIETFYPPFIPQPPSPSSSSLDHPPSSTSTKTIATAV 72

Query: 66  AVTAVGVALISTFFFFLIQRYVIGRKRKTEVVNSGTGSGSGSAVPPAVAQSEFSRVDGNL 125
           AVTAVGVALIST FFFLIQ+YVIGRKRKTE VNSGTGSG    VPPAVAQSEFSRVDGNL
Sbjct: 73  AVTAVGVALISTLFFFLIQKYVIGRKRKTEEVNSGTGSGL--VVPPAVAQSEFSRVDGNL 132

Query: 126 KGFIVDENGLDVIYWKKLEQRKSKNSFDRDVEGNVKENRTKKSEPVQEIPLLRGKSSTSH 185
           KGFIVDENGLDVIYWK+LE+RKSKNSFDRDVEGNVKENR KKSEPVQEIPLLRGKSSTSH
Sbjct: 133 KGFIVDENGLDVIYWKRLEKRKSKNSFDRDVEGNVKENRNKKSEPVQEIPLLRGKSSTSH 192

Query: 186 VKIAPEDEDDSRITSPPPPPPPQVNQPPQFTAISVQAVGKSPSSSNLSSTAPTQSTGNQV 245
           VKIAPEDEDD+RITS  PPPPP +NQPPQF   SVQAVGKSPSSSNLSSTAP QST NQV
Sbjct: 193 VKIAPEDEDDTRITSSSPPPPPHINQPPQFAGNSVQAVGKSPSSSNLSSTAPPQSTDNQV 252

Query: 246 PPKQSPMAVPPPPPPIPAKTNSRLPPPPPPIPKTNSRPPPPPPPIQPKTNSAGPPPPPIP 305
           PP QSPMAVPPPPPPIPAKTNSRLPPPPPPIPKTNSRPPPPPPPIQ KTNSAGPPPPPIP
Sbjct: 253 PPTQSPMAVPPPPPPIPAKTNSRLPPPPPPIPKTNSRPPPPPPPIQTKTNSAGPPPPPIP 312

Query: 306 AKANPSAPPPPPPKAGGSKLPLRPAPPKESNKSSAEASSSADNGQVKMKPLHWDKVNTAN 365
           AKANPSAPPPPPPKAGGSKLPLRPAPPKESNKSS EASSSADNGQVKMKPLHWDKVNTAN
Sbjct: 313 AKANPSAPPPPPPKAGGSKLPLRPAPPKESNKSSGEASSSADNGQVKMKPLHWDKVNTAN 372

Query: 366 ADHSMVWDKMTAGSFKFDGDLMEALFGYVATNRKSPRSEASSSAIAVGRNSGPSQTFILE 425
           ADHSMVWDKM+AGSFKFDGDLMEALFGYVATNRKSPRSEASSSA AVGRNSGPSQTFILE
Sbjct: 373 ADHSMVWDKMSAGSFKFDGDLMEALFGYVATNRKSPRSEASSSANAVGRNSGPSQTFILE 432

Query: 426 PKKSQNIAIVIKSLTVPRNEILDALNEGQGLETEVLEKLTRIALTQEEISQILAYKGDPQ 485
           PKKSQNIAIVIKSLTVPRNEILDALNEG GLETEVLEKLTRIALTQEEISQILAY+GDPQ
Sbjct: 433 PKKSQNIAIVIKSLTVPRNEILDALNEGHGLETEVLEKLTRIALTQEEISQILAYRGDPQ 492

Query: 486 KLADAESFLYDLLKSVPSAFTRFNAMLFRLNFTSDILHHKESLQTLESACKELRTRGLFM 545
           KLADAESFLY LLKSVPSAFTRFNAMLFRLNFTS+ILH KESLQTLESACKELRTRGLFM
Sbjct: 493 KLADAESFLYHLLKSVPSAFTRFNAMLFRLNFTSEILHLKESLQTLESACKELRTRGLFM 552

Query: 546 KLLEAILKAGNRLNAGTARGNARAFNLTALRKLSDVRSTDGKTTLLHFVVQEVIRAEGKR 605
           KLLEAILKAGNRLNAGTARGNARAFNLTALRKLSDVRSTDGKTTLLHFVVQEVIRAEGKR
Sbjct: 553 KLLEAILKAGNRLNAGTARGNARAFNLTALRKLSDVRSTDGKTTLLHFVVQEVIRAEGKR 612

Query: 606 CVLNRNKSLSRNSSRSSDNSFSSLENSAAKEDRVKEYMMLGLPVVGGLSSEFSHVKKASA 665
           CVLNRNKSLSRNSSRSSDNSFSSLENS AKED+VKEYMMLGLPVVGGLSSEFSHVKKASA
Sbjct: 613 CVLNRNKSLSRNSSRSSDNSFSSLENSTAKEDKVKEYMMLGLPVVGGLSSEFSHVKKASA 672

Query: 666 IDYESFVKAGTSLTSRTEEIRKLLTQMGNNEGGFAKEMREFLDAAENELKMVREAQTKVM 725
           IDYESFVKAGTSLTSRTEEIRKLLTQMGNNEGGF KEMREFLDA+E+ELK VRE QTKVM
Sbjct: 673 IDYESFVKAGTSLTSRTEEIRKLLTQMGNNEGGFTKEMREFLDASEDELKKVREEQTKVM 732

Query: 726 DLVMKTTEYYQAGSSKDKETNRLQLFIIIKDFLEMVDRVCVEITRDLQRKRSSAVNAGT- 785
           DLVMKTTEYYQAGSSKDKETNRLQLFIIIKDFLEMVDRVCVEITRDLQRKRSSAVNAG+ 
Sbjct: 733 DLVMKTTEYYQAGSSKDKETNRLQLFIIIKDFLEMVDRVCVEITRDLQRKRSSAVNAGSS 792

Query: 786 -GSGSLPGRSKAIFHNLPENFMSDKSRGSSSDTDDEF 818
            GSGS PGRSKAIFHNLPENFMSDKSRGSSSDTDDEF
Sbjct: 793 SGSGSSPGRSKAIFHNLPENFMSDKSRGSSSDTDDEF 827

BLAST of Cucsat.G9521 vs. ExPASy TrEMBL
Match: A0A1S3BEN1 (Formin-like protein OS=Cucumis melo OX=3656 GN=LOC103489049 PE=3 SV=1)

HSP 1 Score: 1399 bits (3621), Expect = 0.0
Identity = 767/817 (93.88%), Postives = 782/817 (95.72%), Query Frame = 0

Query: 6   HLILFLFFLSLSPLSYSQLSPPQNIETYYP--FPQPPSPSSSSVDHPPSSTSTKTIATAV 65
           HL LFLFFLSLSPL Y QL+PPQNIET+YP   PQPPSPSSSS+DHPPSSTSTKTIATAV
Sbjct: 13  HLNLFLFFLSLSPLCYCQLNPPQNIETFYPPFIPQPPSPSSSSLDHPPSSTSTKTIATAV 72

Query: 66  AVTAVGVALISTFFFFLIQRYVIGRKRKTEVVNSGTGSGSGSAVPPAVAQSEFSRVDGNL 125
           AVTAVGVALIST FFFLIQ+YVIGRKRKTE VNSGTGSG    VPPAVAQSEFSRVDGNL
Sbjct: 73  AVTAVGVALISTLFFFLIQKYVIGRKRKTEEVNSGTGSGL--VVPPAVAQSEFSRVDGNL 132

Query: 126 KGFIVDENGLDVIYWKKLEQRKSKNSFDRDVEGNVKENRTKKSEPVQEIPLLRGKSSTSH 185
           KGFIVDENGLDVIYWK+LE+RKSKNSFDRDVEGNVKENR KKSEPVQEIPLLRGKSSTSH
Sbjct: 133 KGFIVDENGLDVIYWKRLEKRKSKNSFDRDVEGNVKENRNKKSEPVQEIPLLRGKSSTSH 192

Query: 186 VKIAPEDEDDSRITSPPPPPPPQVNQPPQFTAISVQAVGKSPSSSNLSSTAPTQSTGNQV 245
           VKIAPEDEDD+RITS  PPPPP +NQPPQF   SVQAVGKSPSSSNLSSTAP QST NQV
Sbjct: 193 VKIAPEDEDDTRITSSSPPPPPHINQPPQFAGNSVQAVGKSPSSSNLSSTAPPQSTDNQV 252

Query: 246 PPKQSPMAVPPPPPPIPAKTNSRLPPPPPPIPKTNSRPPPPPPPIQPKTNSAGPPPPPIP 305
           PP QSPMAVPPPPPPIPAKTNSRLPPPPPPIPKTNSRPPPPPPPIQ KTNSAGPPPPPIP
Sbjct: 253 PPTQSPMAVPPPPPPIPAKTNSRLPPPPPPIPKTNSRPPPPPPPIQTKTNSAGPPPPPIP 312

Query: 306 AKANPSAPPPPPPKAGGSKLPLRPAPPKESNKSSAEASSSADNGQVKMKPLHWDKVNTAN 365
           AKANPSAPPPPPPKAGGSKLPLRPAPPKESNKSS EASSSADNGQVKMKPLHWDKVNTAN
Sbjct: 313 AKANPSAPPPPPPKAGGSKLPLRPAPPKESNKSSGEASSSADNGQVKMKPLHWDKVNTAN 372

Query: 366 ADHSMVWDKMTAGSFKFDGDLMEALFGYVATNRKSPRSEASSSAIAVGRNSGPSQTFILE 425
           ADHSMVWDKM+AGSFKFDGDLMEALFGYVATNRKSPRSEASSSA AVGRNSGPSQTFILE
Sbjct: 373 ADHSMVWDKMSAGSFKFDGDLMEALFGYVATNRKSPRSEASSSANAVGRNSGPSQTFILE 432

Query: 426 PKKSQNIAIVIKSLTVPRNEILDALNEGQGLETEVLEKLTRIALTQEEISQILAYKGDPQ 485
           PKKSQNIAIVIKSLTVPRNEILDALNEG GLETEVLEKLTRIALTQEEISQILAY+GDPQ
Sbjct: 433 PKKSQNIAIVIKSLTVPRNEILDALNEGHGLETEVLEKLTRIALTQEEISQILAYRGDPQ 492

Query: 486 KLADAESFLYDLLKSVPSAFTRFNAMLFRLNFTSDILHHKESLQTLESACKELRTRGLFM 545
           KLADAESFLY LLKSVPSAFTRFNAMLFRLNFTS+ILH KESLQTLESACKELRTRGLFM
Sbjct: 493 KLADAESFLYHLLKSVPSAFTRFNAMLFRLNFTSEILHLKESLQTLESACKELRTRGLFM 552

Query: 546 KLLEAILKAGNRLNAGTARGNARAFNLTALRKLSDVRSTDGKTTLLHFVVQEVIRAEGKR 605
           KLLEAILKAGNRLNAGTARGNARAFNLTALRKLSDVRSTDGKTTLLHFVVQEVIRAEGKR
Sbjct: 553 KLLEAILKAGNRLNAGTARGNARAFNLTALRKLSDVRSTDGKTTLLHFVVQEVIRAEGKR 612

Query: 606 CVLNRNKSLSRNSSRSSDNSFSSLENSAAKEDRVKEYMMLGLPVVGGLSSEFSHVKKASA 665
           CVLNRNKSLSRNSSRSSDNSFSSLENS AKED+VKEYMMLGLPVVGGLSSEFSHVKKASA
Sbjct: 613 CVLNRNKSLSRNSSRSSDNSFSSLENSTAKEDKVKEYMMLGLPVVGGLSSEFSHVKKASA 672

Query: 666 IDYESFVKAGTSLTSRTEEIRKLLTQMGNNEGGFAKEMREFLDAAENELKMVREAQTKVM 725
           IDYESFVKAGTSLTSRTEEIRKLLTQMGNNEGGF KEMREFLDA+E+ELK VRE QTKVM
Sbjct: 673 IDYESFVKAGTSLTSRTEEIRKLLTQMGNNEGGFTKEMREFLDASEDELKKVREEQTKVM 732

Query: 726 DLVMKTTEYYQAGSSKDKETNRLQLFIIIKDFLEMVDRVCVEITRDLQRKRSSAVNAGT- 785
           DLVMKTTEYYQAGSSKDKETNRLQLFIIIKDFLEMVDRVCVEITRDLQRKRSSAVNAG+ 
Sbjct: 733 DLVMKTTEYYQAGSSKDKETNRLQLFIIIKDFLEMVDRVCVEITRDLQRKRSSAVNAGSS 792

Query: 786 -GSGSLPGRSKAIFHNLPENFMSDKSRGSSSDTDDEF 818
            GSGS PGRSKAIFHNLPENFMSDKSRGSSSDTDDEF
Sbjct: 793 SGSGSSPGRSKAIFHNLPENFMSDKSRGSSSDTDDEF 827

BLAST of Cucsat.G9521 vs. ExPASy TrEMBL
Match: A0A6J1HPB5 (Formin-like protein OS=Cucurbita maxima OX=3661 GN=LOC111465458 PE=3 SV=1)

HSP 1 Score: 1139 bits (2946), Expect = 0.0
Identity = 646/826 (78.21%), Postives = 711/826 (86.08%), Query Frame = 0

Query: 9   LFLFFLSLSPLSYSQLSPPQNIETYYPFPQPPSPSSSSVDHPP-------SSTSTKTIAT 68
           L L FLS+SP+SY Q +PPQNIET+YP   PP       DHPP       SS++T+TI T
Sbjct: 18  LLLLFLSVSPVSYCQDTPPQNIETFYPILPPPP---FLPDHPPPPLPPPSSSSTTRTIVT 77

Query: 69  AVAVTAVGVALISTFFFFLIQRYVIGRKRKTEVVNSGTGSGSGSAVPPAVAQSEFSRVDG 128
           AVA+TAVG+ALIST FFFLIQRY++ RK+KTE VNSG G G  S   PAVA++EF+RVDG
Sbjct: 78  AVAITAVGMALISTVFFFLIQRYLVRRKQKTEEVNSGPGQGPVSQ--PAVARNEFARVDG 137

Query: 129 NLKGFIVDENGLDVIYWKKLEQRKSKNSFDR-DVEGNVKENRTKKSEPVQEIPLLRGKSS 188
           NLKGFIVDE+GLDVIYWK+LE+RKSKNSFDR D EGNV+ NR+KKSEPVQEIPLLRGKSS
Sbjct: 138 NLKGFIVDEDGLDVIYWKRLERRKSKNSFDRGDGEGNVQANRSKKSEPVQEIPLLRGKSS 197

Query: 189 TSHVKIAPEDEDDSRITSPPPPPPPQVNQPPQFTAISVQAVGKSPSSSNLSSTAPTQSTG 248
           +SHVKI+PEDE+  R+T PPPPPPP +N PP FT  SVQ VGK  SSS LSS+AP Q T 
Sbjct: 198 SSHVKISPEDEEPRRVTPPPPPPPPHINNPPPFTGKSVQEVGKPSSSSILSSSAPPQPTA 257

Query: 249 NQVPPKQSPMAVP-------PPPPPIPAKTNSRLPPPPPPIP-KTNSRPPPPPPPIQPKT 308
             VPP QS MAVP       PPPPPIPAKT SR PPPPPPI  KTNSRPPPPPP I  KT
Sbjct: 258 IPVPPSQSLMAVPNNKCSVPPPPPPIPAKTVSRPPPPPPPIATKTNSRPPPPPP-IPAKT 317

Query: 309 NSAGPPPPPIPAKANPSAPPPPPPKAGGSKLPLRPAPPKESNKSSAEASSSADNGQVKMK 368
           NSA PPPPPI AKANP+APPPP  KAG SKLPLRPAP KE  KSS E+S+SADNGQVKMK
Sbjct: 318 NSAAPPPPPIVAKANPAAPPPP--KAGSSKLPLRPAPQKE-GKSSGESSTSADNGQVKMK 377

Query: 369 PLHWDKVNTANADHSMVWDKMTAGSFKFDGDLMEALFGYVATNRKSPRSEASSSAIAVGR 428
           PLHWDKVNTANADHSMVWDKM AGSFKFDGDLMEALFGYVATNRKSPRSEA+SS I  GR
Sbjct: 378 PLHWDKVNTANADHSMVWDKMQAGSFKFDGDLMEALFGYVATNRKSPRSEANSSVIPTGR 437

Query: 429 NSGPSQTFILEPKKSQNIAIVIKSLTVPRNEILDALNEGQGLETEVLEKLTRIALTQEEI 488
           NSGPSQTFILEPKKSQNIAIV+KSLT+PRNEILDALNEGQGLET++LEKLTRI LTQEEI
Sbjct: 438 NSGPSQTFILEPKKSQNIAIVVKSLTIPRNEILDALNEGQGLETDILEKLTRITLTQEEI 497

Query: 489 SQILAYKGDPQKLADAESFLYDLLKSVPSAFTRFNAMLFRLNFTSDILHHKESLQTLESA 548
           SQILAYKGDPQKLADAE+FLY LLKSVPSAF RFNAMLFRL F SDI H KESL+ LESA
Sbjct: 498 SQILAYKGDPQKLADAETFLYHLLKSVPSAFARFNAMLFRLTFGSDIHHLKESLEILESA 557

Query: 549 CKELRTRGLFMKLLEAILKAGNRLNAGTARGNARAFNLTALRKLSDVRSTDGKTTLLHFV 608
           CKELRTRGLF+KLLEA+LKAGNRLNAGTARGNARAFNL+ALRKLSDVRSTDGKTTLLHFV
Sbjct: 558 CKELRTRGLFLKLLEAVLKAGNRLNAGTARGNARAFNLSALRKLSDVRSTDGKTTLLHFV 617

Query: 609 VQEVIRAEGKRCVLNRNKSLSRNSSRSSDNSFSSLENSAAKEDRVKEYMMLGLPVVGGLS 668
           VQEVIRAEGKRCVLNRNKSLSRN+S +SD+S S+ +NS++KEDRV EYMMLGLPVVGGLS
Sbjct: 618 VQEVIRAEGKRCVLNRNKSLSRNTSHASDSSVSTSDNSSSKEDRVNEYMMLGLPVVGGLS 677

Query: 669 SEFSHVKKASAIDYESFVKAGTSLTSRTEEIRKLLTQMGNNEGGFAKEMREFLDAAENEL 728
           +EFS+VKKA+ IDYESF   GTSLTSRT EIR+L+ Q+GNN GGF KEMR FL+AAE+EL
Sbjct: 678 AEFSNVKKAATIDYESFANTGTSLTSRTAEIRQLMAQIGNNGGGFGKEMRGFLEAAESEL 737

Query: 729 KMVREAQTKVMDLVMKTTEYYQAGSSKDKETNRLQLFIIIKDFLEMVDRVCVEITRDLQR 788
           K+VRE QTKVM+LVMKTTEYYQAGSS+DKE+NRLQ F I+KDFLEMVDRVCVEI+R+LQ+
Sbjct: 738 KVVREEQTKVMELVMKTTEYYQAGSSRDKESNRLQFFTIVKDFLEMVDRVCVEISRNLQK 797

Query: 789 KRSSAVNAGTGSGSLPGRSKAIFHNLPENFMSDKSRGSSSDTDDEF 818
           +RSS VN G    S P RSKAIF NLP NFMSDKSRGSSSD+D+EF
Sbjct: 798 RRSSTVNVG----SSPVRSKAIFPNLPANFMSDKSRGSSSDSDNEF 830

BLAST of Cucsat.G9521 vs. ExPASy TrEMBL
Match: A0A6J1FPF1 (Formin-like protein OS=Cucurbita moschata OX=3662 GN=LOC111447297 PE=3 SV=1)

HSP 1 Score: 1137 bits (2940), Expect = 0.0
Identity = 649/824 (78.76%), Postives = 709/824 (86.04%), Query Frame = 0

Query: 9   LFLFFLSLSPLSYSQLSPPQNIETYYPFPQPPS-----PSSSSVDHPPSSTSTKTIATAV 68
           L L FLS+SP+SY Q +PPQNIET+YP   PP      P S S    PSS ST+TI TAV
Sbjct: 16  LLLLFLSVSPVSYCQDTPPQNIETFYPILPPPPFLPVHPPSPSPSSSPSS-STRTIVTAV 75

Query: 69  AVTAVGVALISTFFFFLIQRYVIGRKRKTEVVNSGTGSGSGSAVPPAVAQSEFSRVDGNL 128
           A+TAVG+ALIST FFFLIQRY+I RKRKTE VNSG G G  S   PAVA+SEF+RVDGNL
Sbjct: 76  AITAVGMALISTVFFFLIQRYLIRRKRKTEEVNSGPGQGPVSQ--PAVARSEFARVDGNL 135

Query: 129 KGFIVDENGLDVIYWKKLEQRKSKNSFDR-DVEGNVKENRTKKSEPVQEIPLLRGKSSTS 188
           KGFIVDE+GLDVIYWK+LE+RKSKNSFDR D EGNV+ NR+KKSEPVQEIPLLRGKSS+S
Sbjct: 136 KGFIVDEDGLDVIYWKRLERRKSKNSFDRGDGEGNVQANRSKKSEPVQEIPLLRGKSSSS 195

Query: 189 HVKIAPEDEDDSRITSPPPPPPPQVNQPPQFTAISVQAVGKSPSSSNLSSTAPTQSTGNQ 248
           HVKI+PEDE+  R+T PPPPPP  +N PP FT  SVQ VGK  SSS LSS AP Q     
Sbjct: 196 HVKISPEDEEPRRVTPPPPPPPSHINNPPPFTGKSVQEVGKPSSSSILSSPAPPQPAAIP 255

Query: 249 VPPKQSPMAVP-------PPPPPIPAKTNSRLPPPPPPIP-KTNSRPPPPPPPIQPKTNS 308
           VPP QS MAVP       PPPPPIPAKT SR PPPPPPI  KTNSRPPPPPP I  KTNS
Sbjct: 256 VPPSQSLMAVPNNKSSVPPPPPPIPAKTVSRPPPPPPPIATKTNSRPPPPPP-IPAKTNS 315

Query: 309 AGPPPPPIPAKANPSAPPPPPPKAGGSKLPLRPAPPKESNKSSAEASSSADNGQVKMKPL 368
           A PPPPPI AKANP+APPPP  KAG SKLPLRPAP KE  KSS E+S+SADNGQVKMKPL
Sbjct: 316 AAPPPPPIVAKANPAAPPPP--KAGSSKLPLRPAPQKE-GKSSGESSTSADNGQVKMKPL 375

Query: 369 HWDKVNTANADHSMVWDKMTAGSFKFDGDLMEALFGYVATNRKSPRSEASSSAIAVGRNS 428
           HWDKVNTANADHSMVWDKM AGSFKFDGDLMEALFGYVATNRKSPRSEA+SSAI  GRNS
Sbjct: 376 HWDKVNTANADHSMVWDKMQAGSFKFDGDLMEALFGYVATNRKSPRSEANSSAIPTGRNS 435

Query: 429 GPSQTFILEPKKSQNIAIVIKSLTVPRNEILDALNEGQGLETEVLEKLTRIALTQEEISQ 488
           GPSQTFILEPKKSQNIAIV+KSLT+PRNEILDALNEGQGLET+VLEKLTRI LTQEEISQ
Sbjct: 436 GPSQTFILEPKKSQNIAIVVKSLTIPRNEILDALNEGQGLETDVLEKLTRITLTQEEISQ 495

Query: 489 ILAYKGDPQKLADAESFLYDLLKSVPSAFTRFNAMLFRLNFTSDILHHKESLQTLESACK 548
           ILAYKGDPQKLADAE+FLY LLK+VPSAF RFNAMLFRL F SDI H KESL+ LESACK
Sbjct: 496 ILAYKGDPQKLADAETFLYPLLKAVPSAFARFNAMLFRLTFGSDIHHLKESLEILESACK 555

Query: 549 ELRTRGLFMKLLEAILKAGNRLNAGTARGNARAFNLTALRKLSDVRSTDGKTTLLHFVVQ 608
           ELRTRGLF+KLLEA+LKAGNRLNAGTARGNARAFNL+ALRKLSDVRSTDGKTTLLHFVVQ
Sbjct: 556 ELRTRGLFLKLLEAVLKAGNRLNAGTARGNARAFNLSALRKLSDVRSTDGKTTLLHFVVQ 615

Query: 609 EVIRAEGKRCVLNRNKSLSRNSSRSSDNSFSSLENSAAKEDRVKEYMMLGLPVVGGLSSE 668
           EVIRAEGKRCVLNRNKSLSRN+S +SD+S S+ +NS++KEDRV EYMMLGLPVVGGLS+E
Sbjct: 616 EVIRAEGKRCVLNRNKSLSRNTSHASDSSVSTSDNSSSKEDRVNEYMMLGLPVVGGLSAE 675

Query: 669 FSHVKKASAIDYESFVKAGTSLTSRTEEIRKLLTQMGNNEGGFAKEMREFLDAAENELKM 728
           FS+VKKA+ IDYESF   GTSLT+RT EIR+L+ Q+GNN GGF KEMR FL+AAE+ELK+
Sbjct: 676 FSNVKKAATIDYESFANTGTSLTNRTAEIRQLMAQIGNNGGGFGKEMRGFLEAAESELKV 735

Query: 729 VREAQTKVMDLVMKTTEYYQAGSSKDKETNRLQLFIIIKDFLEMVDRVCVEITRDLQRKR 788
           VRE QTKVM+LVMKTTEYYQAGSS+DKE+NRLQ FII+KDFLEMVDRVCVEI+R+LQ++R
Sbjct: 736 VREEQTKVMELVMKTTEYYQAGSSRDKESNRLQFFIIVKDFLEMVDRVCVEISRNLQKRR 795

Query: 789 SSAVNAGTGSGSLPGRSKAIFHNLPENFMSDKSRGSSSDTDDEF 818
           SS VN G    S P RSKAIF NLP NFMSDKSRGSSSD+D+EF
Sbjct: 796 SSTVNVG----SSPVRSKAIFPNLPPNFMSDKSRGSSSDSDNEF 828

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O486826.1e-16547.80Formin-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=FH4 PE=1 SV=2[more]
O045322.1e-15745.30Formin-like protein 8 OS=Arabidopsis thaliana OX=3702 GN=FH8 PE=1 SV=1[more]
A3AB673.8e-13540.00Formin-like protein 16 OS=Oryza sativa subsp. japonica OX=39947 GN=FH16 PE=2 SV=... [more]
Q9XIE01.8e-12949.84Formin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=FH7 PE=2 SV=1[more]
Q10Q998.1e-7737.19Formin-like protein 8 OS=Oryza sativa subsp. japonica OX=39947 GN=FH8 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_004135469.10.099.39formin-like protein 4 [Cucumis sativus] >KGN51846.1 hypothetical protein Csa_008... [more]
XP_008446271.10.093.88PREDICTED: formin-like protein 4 [Cucumis melo] >KAA0034348.1 formin-like protei... [more]
XP_038891749.10.085.82formin-like protein 4 [Benincasa hispida][more]
XP_022965655.10.078.21formin-like protein 4 [Cucurbita maxima][more]
KAG6600142.10.078.76Formin-like protein 8, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
A0A0A0KVR00.099.39Formin-like protein OS=Cucumis sativus OX=3659 GN=Csa_5G603920 PE=3 SV=1[more]
A0A5D3CUJ40.093.88Formin-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G0... [more]
A0A1S3BEN10.093.88Formin-like protein OS=Cucumis melo OX=3656 GN=LOC103489049 PE=3 SV=1[more]
A0A6J1HPB50.078.21Formin-like protein OS=Cucurbita maxima OX=3661 GN=LOC111465458 PE=3 SV=1[more]
A0A6J1FPF10.078.76Formin-like protein OS=Cucurbita moschata OX=3662 GN=LOC111447297 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 698..718
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 611..630
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 32..46
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 334..348
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 148..350
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 247..323
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 148..171
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 32..53
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 211..246
NoneNo IPR availablePANTHERPTHR23213FORMIN-RELATEDcoord: 7..814
NoneNo IPR availablePANTHERPTHR23213:SF354FORMIN-LIKE PROTEIN 4coord: 7..814
NoneNo IPR availableSUPERFAMILY101447Formin homology 2 domain (FH2 domain)coord: 282..773
IPR015425Formin, FH2 domainSMARTSM00498it6_sourcecoord: 341..774
e-value: 5.2E-101
score: 351.5
IPR015425Formin, FH2 domainPFAMPF02181FH2coord: 344..760
e-value: 1.6E-101
score: 340.2
IPR015425Formin, FH2 domainPROSITEPS51444FH2coord: 341..785
score: 47.147152
IPR042201Formin, FH2 domain superfamilyGENE3D1.20.58.2220Formin, FH2 domaincoord: 351..781
e-value: 1.3E-92
score: 312.7

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G9521.T1Cucsat.G9521.T1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane