Cucsat.G5649 (gene) Cucumber (B10) v3

BLAST of Cucsat.G5649 vs. ExPASy Swiss-Prot
Match: Q9FIZ7 (5-oxoprolinase OS=Arabidopsis thaliana OX=3702 GN=OXP1 PE=1 SV=1)

HSP 1 Score: 2140.2 bits (5544), Expect = 0.0e+00
Identity = 1044/1265 (82.53%), Postives = 1149/1265 (90.83%), Query Frame = 0

Query: 1    MGSNNEEKLRFCIDRGGTFTDVYAEIPGRPDGKVFKLLSVDPSNYDDAPVEGIRRILEEY 60
            MG+  E KLRFCIDRGGTFTDVYAEIPG  DG V KLLSVDPSNYDDAPVEGIRRILEEY
Sbjct: 1    MGTVIEGKLRFCIDRGGTFTDVYAEIPGHSDGHVLKLLSVDPSNYDDAPVEGIRRILEEY 60

Query: 61   TGKKIPRTSKIPTQNIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI 120
            TGKKIPRTSKIPT  I+WIRMGTTVATNALLERKGERIALCVTKGF+DLLQIGNQARPDI
Sbjct: 61   TGKKIPRTSKIPTDKIQWIRMGTTVATNALLERKGERIALCVTKGFKDLLQIGNQARPDI 120

Query: 121  FDLTVSKPSNLYEDVVEVDERVELIHSKGDGNQDSSTYVEGVSGELIRIVKTLNEEALKP 180
            FDLTV+KPSNLYE+V+EVDERV L  +  D + D  + ++GVSGE +R+VK  + E LKP
Sbjct: 121  FDLTVAKPSNLYEEVIEVDERVVL--ALEDDDDDEGSLIKGVSGEFLRVVKPFDGEGLKP 180

Query: 181  LLNDLLQRGIGCLAVVLMHSYTYPQHELALEKLALSMGFKHVSLSSALTPMVRAVPRGLT 240
            LL  LL +GI CLAVVLMHSYTYP+HE+ +EKLAL MGF+HVSLSSALTPMVRAVPRGLT
Sbjct: 181  LLKGLLDKGISCLAVVLMHSYTYPKHEMDVEKLALEMGFRHVSLSSALTPMVRAVPRGLT 240

Query: 241  ASVDAYLTPVIKEYLSGFMSKFDESSGKVNVLFMQSDGGLAPESRFSGHKAVLSGPAGGV 300
            A+VDAYLTPVIKEYLSGF+SKFD+  GKVNVLFMQSDGGLAPESRFSGHKAVLSGPAGGV
Sbjct: 241  ATVDAYLTPVIKEYLSGFISKFDDDLGKVNVLFMQSDGGLAPESRFSGHKAVLSGPAGGV 300

Query: 301  VGYSQTLFELETTKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVAA 360
            VGYSQTLF LET KPLIGFDMGGTSTDVSRY GSYEQV+ETQIAG IIQAPQLDINTVAA
Sbjct: 301  VGYSQTLFGLETEKPLIGFDMGGTSTDVSRYDGSYEQVIETQIAGTIIQAPQLDINTVAA 360

Query: 361  GGGSKLKFQFGAFRVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPNE 420
            GGGSKLKFQFGAFRVGP+SVGAHPGPVCYRKGGELAVTDANLVLGFVIPD+FPSIFGPNE
Sbjct: 361  GGGSKLKFQFGAFRVGPDSVGAHPGPVCYRKGGELAVTDANLVLGFVIPDYFPSIFGPNE 420

Query: 421  DQPLDIEATRGEFEKLATEINSYRKNQDPSSKPMTIEEIALGFVNVANETMCRPIRQLTE 480
            DQPLD+ ATR  FEKLA +IN YRK+QDPS+K M++EEIA+GFV+VANETMCRPIRQLTE
Sbjct: 421  DQPLDVAATREAFEKLAGQINIYRKSQDPSAKDMSVEEIAMGFVSVANETMCRPIRQLTE 480

Query: 481  MKGHETKNHALACFGGAGPQHACAIARLLGMKEIFIHRFCGILSAYGMGLADVVEEEQEP 540
            MKGHETKNHALACFGGAGPQHACAIAR LGMKE+ +HR+CGILSAYGMGLADV+E+ QEP
Sbjct: 481  MKGHETKNHALACFGGAGPQHACAIARSLGMKEVLVHRYCGILSAYGMGLADVIEDAQEP 540

Query: 541  YSAVYCSKSVQEVSRREASLLKQVKHKLRSQGFREGSINTETYLNLRYDGTDTAIMVKSQ 600
            YSAVY  +S+ EV RRE  LL++V+ KL+ QGF +G+I+TETYLNLRYDGTDTAIMVK +
Sbjct: 541  YSAVYGPESLSEVFRRETVLLREVREKLQEQGFGDGNISTETYLNLRYDGTDTAIMVKGK 600

Query: 601  RVDNGIEFDFAAEFEKLFQQEYGFKLQNRNILICDIRVRGVGVTNVLKPRAFEGLSGDPK 660
            +  +G  FD+AAEF KLF+QEYGFKLQNRN+LICD+RVRG+GVT++LKPRA E     PK
Sbjct: 601  KTGDGSAFDYAAEFLKLFEQEYGFKLQNRNLLICDVRVRGIGVTSILKPRAVEAAPVTPK 660

Query: 661  IEGHYRVYFGNGWQDTPLFKLDNLGFGYIIPGPAIIMNGNSTVIVEPSCKATVTKYGNIK 720
            +E HY+VYF  GW DTPLFKL+NLGFG+ I GPAIIMNGNSTVIVEP CKA +TKYGNIK
Sbjct: 661  VERHYKVYFEGGWHDTPLFKLENLGFGHEILGPAIIMNGNSTVIVEPQCKAIITKYGNIK 720

Query: 721  IEIDSTFCTKKVSEKVADVVQLSIFNHQFMGIAEQMGRTLQRTSISTNIKERLDFSCALF 780
            IE++    + K++E VADVVQLSIFNH+FMGIAEQMGRTLQRTSISTNIKERLDFSCALF
Sbjct: 721  IEVEPATSSVKLAENVADVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCALF 780

Query: 781  GPDGGLVANAPHVPVHLGAMSSTVRWQIDFWGDNLNEGDVLVTNHPCAGGSHLPDITVIT 840
             PDGGLVANAPHVPVHLGAMSSTVRWQ+  WG+NLNEGDVLVTNHPCAGGSHLPDITVIT
Sbjct: 781  SPDGGLVANAPHVPVHLGAMSSTVRWQLKHWGENLNEGDVLVTNHPCAGGSHLPDITVIT 840

Query: 841  PVFDNGKLIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVEKGIFQEEGIN 900
            PVFD GKL+FFVASRGHHAE+GGITPGSMPPFSK+IWEEGAAIKAFK+VEKG+FQEEGI 
Sbjct: 841  PVFDKGKLVFFVASRGHHAEVGGITPGSMPPFSKAIWEEGAAIKAFKVVEKGVFQEEGIV 900

Query: 901  KLLQFPSSDEGV--IPGTRRLQDNLSDLHAQVAANHRGISLIKELIVQYGLNIVQAYMTY 960
            KLLQFPSSDE    IPGTRR+QDNLSDL AQ+AAN RGISLIKELI QYGL  VQAYM Y
Sbjct: 901  KLLQFPSSDETTTKIPGTRRIQDNLSDLQAQIAANQRGISLIKELIEQYGLGTVQAYMKY 960

Query: 961  VQLNAEEAVREMLKSVASRVSSNSAKYVEGGSIAIEEEDYMDDGSAIHLKLTIDPHKGEA 1020
            VQLNAEEAVREMLKSVA+RVSS +     G S+ IEEEDYMDDGS IHLKLTID  KGEA
Sbjct: 961  VQLNAEEAVREMLKSVANRVSSETPNSRVGNSVTIEEEDYMDDGSIIHLKLTIDADKGEA 1020

Query: 1021 NFDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPPGSFLSP 1080
            +FDF+GTSPEVYGNWNAPEAVT+AAVIYCLRC+V+VDIPLNQGCLAPV+I IP GSFLSP
Sbjct: 1021 SFDFTGTSPEVYGNWNAPEAVTSAAVIYCLRCLVNVDIPLNQGCLAPVEIRIPAGSFLSP 1080

Query: 1081 SEKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGPS 1140
            SEKAA+VGGNVLTSQR+TDV+LTAFQACACSQGCMNNLTFGDDTFGYYETIGGG GAGP+
Sbjct: 1081 SEKAAVVGGNVLTSQRVTDVVLTAFQACACSQGCMNNLTFGDDTFGYYETIGGGCGAGPT 1140

Query: 1141 WHGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGSGVYKGGDGLVREIEFKQP 1200
            W+GTSGVQCHMTNTRMTDPEIFEQRYPVLLH F LRENSGG+G++KGGDGLVREIEF++P
Sbjct: 1141 WNGTSGVQCHMTNTRMTDPEIFEQRYPVLLHRFGLRENSGGNGLHKGGDGLVREIEFRKP 1200

Query: 1201 VVVSILSERRVHAPRGLKGGKDGARGANFLVRKDGRRVYLGGKNTITVKAGEILQILTPG 1260
            VVVSILSERRVH+PRGL GG++G RGAN+L+ KD RR+YLGGKNT+ V+AGEILQILTPG
Sbjct: 1201 VVVSILSERRVHSPRGLNGGQNGLRGANYLITKDKRRIYLGGKNTVHVEAGEILQILTPG 1260

Query: 1261 GGGWG 1264
            GGG+G
Sbjct: 1261 GGGFG 1263

BLAST of Cucsat.G5649 vs. ExPASy Swiss-Prot
Match: O14841 (5-oxoprolinase OS=Homo sapiens OX=9606 GN=OPLAH PE=1 SV=3)

HSP 1 Score: 1422.5 bits (3681), Expect = 0.0e+00
Identity = 727/1272 (57.15%), Postives = 917/1272 (72.09%), Query Frame = 0

Query: 1    MGSNNEEKLRFCIDRGGTFTDVYAEIPGRPDGKVFKLLSVDPSNYDDAPVEGIRRILEEY 60
            MGS  E +  F IDRGGTFTDV+A+ PG    +V KLLS DP+NY DAP EGIRRILE+ 
Sbjct: 1    MGS-PEGRFHFAIDRGGTFTDVFAQCPG-GHVRVLKLLSEDPANYADAPTEGIRRILEQE 60

Query: 61   TGKKIPRTSKIPTQNIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI 120
             G  +PR   + + +I  IRMGTTVATNALLERKGER+AL VT+GFRDLL IG QAR D+
Sbjct: 61   AGMLLPRDQPLDSSHIASIRMGTTVATNALLERKGERVALLVTRGFRDLLHIGTQARGDL 120

Query: 121  FDLTVSKPSNLYEDVVEVDERVELIHSKGDGNQDSSTYVEGVSGELIRIVKTLNEEALKP 180
            FDL V  P  LYE+V+EVDERV ++H    G   + T V+G +G+L+ + + ++  AL+ 
Sbjct: 121  FDLAVPMPEVLYEEVLEVDERV-VLHR---GEAGTGTPVKGRTGDLLEVQQPVDLGALRG 180

Query: 181  LLNDLLQRGIGCLAVVLMHSYTYPQHELALEKLALSMGFKHVSLSSALTPMVRAVPRGLT 240
             L  LL RGI  LAVVLMHSYT+ QHE  +  LA  +GF HVSLSS   PMVR VPRG T
Sbjct: 181  KLEGLLSRGIRSLAVVLMHSYTWAQHEQQVGVLARELGFTHVSLSSEAMPMVRIVPRGHT 240

Query: 241  ASVDAYLTPVIKEYLSGFMSKFDESSGKVNVLFMQSDGGLAPESRFSGHKAVLSGPAGGV 300
            A  DAYLTP I+ Y+ GF   F      V VLFM+SDGGLAP   FSG  AVLSGPAGGV
Sbjct: 241  ACADAYLTPAIQRYVQGFCRGFQGQLKDVQVLFMRSDGGLAPMDTFSGSSAVLSGPAGGV 300

Query: 301  VGYSQTLFELETTKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVAA 360
            VGYS T ++ E  +P+IGFDMGGTSTDVSRYAG +E V E   AG  +QAPQLDINTVAA
Sbjct: 301  VGYSATTYQQEGGQPVIGFDMGGTSTDVSRYAGEFEHVFEASTAGVTLQAPQLDINTVAA 360

Query: 361  GGGSKLKFQFGAFRVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPNE 420
            GGGS+L F+ G F VGPES GAHPGP CYRKGG + VTDANLVLG ++P  FP IFGP E
Sbjct: 361  GGGSRLFFRSGLFVVGPESAGAHPGPACYRKGGPVTVTDANLVLGRLLPASFPCIFGPGE 420

Query: 421  DQPLDIEATRGEFEKLATEINSYRKNQDPSSKPMTIEEIALGFVNVANETMCRPIRQLTE 480
            +QPL  EA+R   E +ATE+NS+  N    + P+++EE+A+GFV VANE MCRPIR LT+
Sbjct: 421  NQPLSPEASRKALEAVATEVNSFLTNGPCPASPLSLEEVAMGFVRVANEAMCRPIRALTQ 480

Query: 481  MKGHETKNHALACFGGAGPQHACAIARLLGMKEIFIHRFCGILSAYGMGLADVVEEEQEP 540
             +GH+   H LACFGGAG QHACAIAR LGM  + IHR  G+LSA G+ LADVV E QEP
Sbjct: 481  ARGHDPSAHVLACFGGAGGQHACAIARALGMDTVHIHRHSGLLSALGLALADVVHEAQEP 540

Query: 541  YSAVYCSKSVQEVSRREASLLKQVKHKLRSQGFREGSINTETYLNLRYDGTDTAIMVKSQ 600
             S +Y  ++  ++ +R + L +Q    L++QGF    I+TE++L+LRY GTD A+MV + 
Sbjct: 541  CSLLYAPETFVQLDQRLSRLEEQCVDALQAQGFPRSQISTESFLHLRYQGTDCALMVSAH 600

Query: 601  R----VDNGIEFDFAAEFEKLFQQEYGFKLQNRNILICDIRVRGVGVTNVLKPRAFEGLS 660
            +      +    DF A F + + +E+GF +  R +++ D+RVRG G + +    A +  +
Sbjct: 601  QHPATARSPRAGDFGAAFVERYMREFGFVIPERPVVVDDVRVRGTGRSGLRLEDAPKAQT 660

Query: 661  GDPKIEGHYRVYFGNGWQDTPLFKLDNLGFGYIIPGPAIIMNGNSTVIVEPSCKATVTKY 720
            G P+++   + YF  G+Q+TP++ L  LG+G+ + GP +I++ NST++VEP C+A VTK 
Sbjct: 661  GPPRVDKMTQCYFEGGYQETPVYLLAELGYGHKLHGPCLIIDSNSTILVEPGCQAEVTKT 720

Query: 721  GNIKIEIDSTFCTKKVSEKVADVVQLSIFNHQFMGIAEQMGRTLQRTSISTNIKERLDFS 780
            G+I I + +            D +QLSIF+H+FM IAEQMGR LQRT+ISTNIKERLDFS
Sbjct: 721  GDICISVGAE--VPGTVGPQLDPIQLSIFSHRFMSIAEQMGRILQRTAISTNIKERLDFS 780

Query: 781  CALFGPDGGLVANAPHVPVHLGAMSSTVRWQIDFWGDNLNEGDVLVTNHPCAGGSHLPDI 840
            CALFGPDGGLV+NAPH+PVHLGAM  TV++QI   G +L+ GDVL++NHP AGGSHLPD+
Sbjct: 781  CALFGPDGGLVSNAPHIPVHLGAMQETVQFQIQHLGADLHPGDVLLSNHPSAGGSHLPDL 840

Query: 841  TVITPVFDNGKL--IFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVEKGIF 900
            TVITPVF  G+   +F+VASRGHHA+IGGITPGSMPP S  + +EGA   +FKLV+ G+F
Sbjct: 841  TVITPVFWPGQTRPVFYVASRGHHADIGGITPGSMPPHSTMLQQEGAVFLSFKLVQGGVF 900

Query: 901  QEEGINKLLQFPSSDEGVIP---GTRRLQDNLSDLHAQVAANHRGISLIKELIVQYGLNI 960
            QEE + + L+ P    G +P   GTR L DNLSDL AQVAAN +GI L+ ELI QYGL++
Sbjct: 901  QEEAVTEALRAP----GKVPNCSGTRNLHDNLSDLRAQVAANQKGIQLVGELIGQYGLDV 960

Query: 961  VQAYMTYVQLNAEEAVREMLKSVASRVSSNSAKYVEGGSIAIEEEDYMDDGSAIHLKLTI 1020
            VQAYM ++Q NAE AVR+ML++        +++   G  + +  ED+MDDGS I L++ I
Sbjct: 961  VQAYMGHIQANAELAVRDMLRAF------GTSRQARGLPLEVSSEDHMDDGSPIRLRVQI 1020

Query: 1021 DPHKGEANFDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIP 1080
               +G A FDFSGT PEV+GN NAP AVT +A+IYCLRC+V  DIPLNQGCLAPV++ IP
Sbjct: 1021 SLSQGSAVFDFSGTGPEVFGNLNAPRAVTLSALIYCLRCLVGRDIPLNQGCLAPVRVVIP 1080

Query: 1081 PGSFLSPSEKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGG 1140
             GS L PS +AA+VGGNVLTSQR+ DVIL AF ACA SQGCMNN+T G+   GYYET+ G
Sbjct: 1081 RGSILDPSPEAAVVGGNVLTSQRVVDVILGAFGACAASQGCMNNVTLGNAHMGYYETVAG 1140

Query: 1141 GSGAGPSWHGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGSGVYKGGDGLVR 1200
            G+GAGPSWHG SGV  HMTNTR+TDPEI E RYPV+L  F LR  SGG G ++GGDG+ R
Sbjct: 1141 GAGAGPSWHGRSGVHSHMTNTRITDPEILESRYPVILRRFELRRGSGGRGRFRGGDGVTR 1200

Query: 1201 EIEFKQPVVVSILSERRVHAPRGLKGGKDGARGANFLVRKDGRRVYLGGKNTITVKAGEI 1260
            E+ F++  ++S+L+ERR   P GL GG+ GARG N L+RK+GR V LGGK ++TV  G++
Sbjct: 1201 ELLFREEALLSVLTERRAFRPYGLHGGEPGARGLNLLIRKNGRTVNLGGKTSVTVYPGDV 1254

Query: 1261 LQILTPGGGGWG 1264
              + TPGGGG+G
Sbjct: 1261 FCLHTPGGGGYG 1254

BLAST of Cucsat.G5649 vs. ExPASy Swiss-Prot
Match: Q8K010 (5-oxoprolinase OS=Mus musculus OX=10090 GN=Oplah PE=1 SV=1)

HSP 1 Score: 1422.1 bits (3680), Expect = 0.0e+00
Identity = 724/1269 (57.05%), Postives = 915/1269 (72.10%), Query Frame = 0

Query: 1    MGSNNEEKLRFCIDRGGTFTDVYAEIPGRPDGKVFKLLSVDPSNYDDAPVEGIRRILEEY 60
            MGS  EE+  F IDRGGTFTDV+A+ PG    +V KLLS DP+NY DAP EGIRRILE+ 
Sbjct: 1    MGS-PEERFHFAIDRGGTFTDVFAQCPG-GHVRVLKLLSEDPANYADAPTEGIRRILEQE 60

Query: 61   TGKKIPRTSKIPTQNIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI 120
             G  +PR   + T +I  IRMGTTVATNALLER+GER+AL VT+GFRDLL IG QARPD+
Sbjct: 61   RGVLLPRGRPLDTSHIASIRMGTTVATNALLERQGERVALLVTRGFRDLLHIGTQARPDL 120

Query: 121  FDLTVSKPSNLYEDVVEVDERVELIHSKGDGNQDSSTYVEGVSGELIRIVKTLNEEALKP 180
            FDL V  P  LYE+VVEVDERV L      G   + + V+G +G+L+ I + ++  AL+ 
Sbjct: 121  FDLAVPMPEVLYEEVVEVDERVLLYR----GEPGAGSPVKGCTGDLLEIQQPVDLAALRG 180

Query: 181  LLNDLLQRGIGCLAVVLMHSYTYPQHELALEKLALSMGFKHVSLSSALTPMVRAVPRGLT 240
             L  LL RGI  LAVVLMHSYT+ QHE  +  LA  +GF HVSLSS + PMVR VPRG T
Sbjct: 181  KLEGLLTRGIHSLAVVLMHSYTWAQHEQQVGTLARELGFTHVSLSSEVMPMVRIVPRGHT 240

Query: 241  ASVDAYLTPVIKEYLSGFMSKFDESSGKVNVLFMQSDGGLAPESRFSGHKAVLSGPAGGV 300
            A  DAYLTP I+ Y+ GF   F      V VLFM+SDGGLAP   FSG +AVLSGPAGGV
Sbjct: 241  ACADAYLTPTIQRYVQGFRRGFQGQLKNVQVLFMRSDGGLAPMDAFSGSRAVLSGPAGGV 300

Query: 301  VGYSQTLFELETTKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVAA 360
            VGYS T ++LE  +P+IGFDMGGTSTDVSRYAG +E V E   AG  +QAPQLDINTVAA
Sbjct: 301  VGYSTTTYQLEGGQPVIGFDMGGTSTDVSRYAGEFEHVFEASTAGVTLQAPQLDINTVAA 360

Query: 361  GGGSKLKFQFGAFRVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPNE 420
            GGGS+L F+ G F VGPES GAHPGP CYRKGG + VTDANLVLG ++P  FP IFGP E
Sbjct: 361  GGGSRLFFRSGLFVVGPESAGAHPGPACYRKGGPVTVTDANLVLGRLLPASFPCIFGPGE 420

Query: 421  DQPLDIEATRGEFEKLATEINSYRKNQDPSSKPMTIEEIALGFVNVANETMCRPIRQLTE 480
            DQPL  EA+R   E +A E+NS+  +    +  +++EE+A+GFV VANE MCRPIR LT+
Sbjct: 421  DQPLSPEASRKALEAVAMEVNSFLASGPCPASQLSLEEVAMGFVRVANEAMCRPIRALTQ 480

Query: 481  MKGHETKNHALACFGGAGPQHACAIARLLGMKEIFIHRFCGILSAYGMGLADVVEEEQEP 540
             +GH+   H LACFGGAG QHACAIAR LGM  + IHR  G+LSA G+ LADVV E QEP
Sbjct: 481  ARGHDPSAHVLACFGGAGGQHACAIARALGMDTVHIHRHSGLLSALGLALADVVHEAQEP 540

Query: 541  YSAVYCSKSVQEVSRREASLLKQVKHKLRSQGFREGSINTETYLNLRYDGTDTAIMVKSQ 600
             S  Y  ++  ++ +R + L +Q    L++QGF    I+TE++L+LRY GTD A+MV + 
Sbjct: 541  CSLSYTPETFAQLDQRLSRLEEQCVDALQAQGFSRSQISTESFLHLRYQGTDCALMVSAN 600

Query: 601  R----VDNGIEFDFAAEFEKLFQQEYGFKLQNRNILICDIRVRGVGVTNVLKPRAFEGLS 660
            +      +    DF A F + + +E+GF +  R++++ D+RVRG G + +      +  S
Sbjct: 601  QHPATTCSPRAGDFGAAFVERYMREFGFIIPERSVVVDDVRVRGTGRSGLQLEETSKIQS 660

Query: 661  GDPKIEGHYRVYFGNGWQDTPLFKLDNLGFGYIIPGPAIIMNGNSTVIVEPSCKATVTKY 720
            G P +E   + YF  G+Q+TP++ L  LG+G+ + GP +I++ NST++VEP C+A V + 
Sbjct: 661  GPPHVEKVTQCYFEGGYQETPVYLLGELGYGHQLQGPCLIIDNNSTILVEPGCQAEVIET 720

Query: 721  GNIKIEIDSTFCTKKVSEKVADVVQLSIFNHQFMGIAEQMGRTLQRTSISTNIKERLDFS 780
            G+I+I + +      + +   D +QLSIF+H+FM IAEQMGR LQRT+ISTNIKERLDFS
Sbjct: 721  GDIRISVGAE--APSMIDTKLDPIQLSIFSHRFMSIAEQMGRILQRTAISTNIKERLDFS 780

Query: 781  CALFGPDGGLVANAPHVPVHLGAMSSTVRWQIDFWGDNLNEGDVLVTNHPCAGGSHLPDI 840
            CALFGPDGGLV+NAPH+PVHLGAM  TV++QI   G +L+ GDVL++NHP AGGSHLPD+
Sbjct: 781  CALFGPDGGLVSNAPHIPVHLGAMQETVQFQIQHLGADLHPGDVLLSNHPSAGGSHLPDL 840

Query: 841  TVITPVFDNG--KLIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVEKGIF 900
            TVITPVF  G  + +F+VASRGHHA+IGGITPGSMPP S ++ +EGA   +FKLV+ G+F
Sbjct: 841  TVITPVFWPGQSRPVFYVASRGHHADIGGITPGSMPPHSTTLQQEGAVFLSFKLVQGGVF 900

Query: 901  QEEGINKLLQFPSSDEGVIPGTRRLQDNLSDLHAQVAANHRGISLIKELIVQYGLNIVQA 960
            QEE + + LQ P    G   GTR L DNLSDL AQVAAN +GI L+ ELI QYGL++VQA
Sbjct: 901  QEEAVTEALQAPGKISG-CSGTRNLHDNLSDLRAQVAANQKGIQLVGELIGQYGLDVVQA 960

Query: 961  YMTYVQLNAEEAVREMLKSVASRVSSNSAKYVEGGSIAIEEEDYMDDGSAIHLKLTIDPH 1020
            YM ++Q NAE AVR+ML++        +++   G  + +  +D+MDDGS I L + I+ +
Sbjct: 961  YMGHIQANAELAVRDMLRAF------GTSRQARGLPLEVSAKDHMDDGSPICLHVQINLN 1020

Query: 1021 KGEANFDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPPGS 1080
            +G A FDFSG+  EV+GN NAP A+T +A+IYCLRC+V  DIPLNQGCLAPV++ IP GS
Sbjct: 1021 QGSAVFDFSGSGSEVFGNLNAPRAITLSALIYCLRCLVGRDIPLNQGCLAPVQVIIPKGS 1080

Query: 1081 FLSPSEKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSG 1140
             L PS +AA+VGGNVLTSQR+ DVIL AF ACA SQGCMNN+T G+   GYYET+ GG+G
Sbjct: 1081 ILDPSPEAAVVGGNVLTSQRVVDVILGAFGACAASQGCMNNVTLGNARMGYYETVAGGAG 1140

Query: 1141 AGPSWHGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGSGVYKGGDGLVREIE 1200
            AGP WHG SGV  HMTNTR+TDPEI E RYPV+L  F LR  SGG G ++GGDG+VRE+ 
Sbjct: 1141 AGPGWHGRSGVHSHMTNTRITDPEILESRYPVILRRFELRPGSGGRGRFRGGDGVVRELV 1200

Query: 1201 FKQPVVVSILSERRVHAPRGLKGGKDGARGANFLVRKDGRRVYLGGKNTITVKAGEILQI 1260
            F++  ++S+L+ERR   P GL GG+ G RG N L+RKDGR V LGGK ++TV  G+   +
Sbjct: 1201 FREEALLSVLTERRAFQPYGLHGGEPGTRGLNLLIRKDGRTVNLGGKTSVTVYPGDAFCL 1254

Query: 1261 LTPGGGGWG 1264
             TPGGGG+G
Sbjct: 1261 HTPGGGGYG 1254

BLAST of Cucsat.G5649 vs. ExPASy Swiss-Prot
Match: P97608 (5-oxoprolinase OS=Rattus norvegicus OX=10116 GN=Oplah PE=1 SV=2)

HSP 1 Score: 1419.4 bits (3673), Expect = 0.0e+00
Identity = 723/1269 (56.97%), Postives = 914/1269 (72.03%), Query Frame = 0

Query: 1    MGSNNEEKLRFCIDRGGTFTDVYAEIPGRPDGKVFKLLSVDPSNYDDAPVEGIRRILEEY 60
            MGS  E +  F IDRGGTFTDV+A+ PG    +V KLLS DP+NY DAP EGIRRILE+ 
Sbjct: 1    MGS-PEGRFHFAIDRGGTFTDVFAQCPG-GHVRVLKLLSEDPANYADAPTEGIRRILEQE 60

Query: 61   TGKKIPRTSKIPTQNIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI 120
             G  +PR   + T  I  IRMGTTVATNALLER+GER+AL VT+GFRDLL IG QARPD+
Sbjct: 61   EGVLLPRGRPLDTSRIASIRMGTTVATNALLERQGERVALLVTRGFRDLLHIGTQARPDL 120

Query: 121  FDLTVSKPSNLYEDVVEVDERVELIHSKGDGNQDSSTYVEGVSGELIRIVKTLNEEALKP 180
            FDL V  P  LYE+V+EVDERV L      G   + + V+G +G+L+ I + ++ EAL+ 
Sbjct: 121  FDLAVPMPEVLYEEVLEVDERVVLYR----GEPGAGSPVKGRTGDLLEIQQPVDLEALRG 180

Query: 181  LLNDLLQRGIGCLAVVLMHSYTYPQHELALEKLALSMGFKHVSLSSALTPMVRAVPRGLT 240
             L  LL RGI  LAVVLMHSYT+ QHE  +  LA  +GF HVSLSS + PMVR VPRG T
Sbjct: 181  KLEGLLSRGIHSLAVVLMHSYTWAQHEQQVGTLARELGFTHVSLSSEVMPMVRIVPRGHT 240

Query: 241  ASVDAYLTPVIKEYLSGFMSKFDESSGKVNVLFMQSDGGLAPESRFSGHKAVLSGPAGGV 300
            A  DAYLTP I+ Y+ GF   F      V VLFM+SDGGLAP   FSG +AVLSGPAGGV
Sbjct: 241  ACADAYLTPTIQRYVQGFRRGFQGQLKNVQVLFMRSDGGLAPMDAFSGSRAVLSGPAGGV 300

Query: 301  VGYSQTLFELETTKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVAA 360
            VGYS T + LE  +P+IGFDMGGTSTDVSRYAG +E V E   AG  +QAPQLDINTVAA
Sbjct: 301  VGYSATTYHLEGGQPVIGFDMGGTSTDVSRYAGEFEHVFEASTAGVTLQAPQLDINTVAA 360

Query: 361  GGGSKLKFQFGAFRVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPNE 420
            GGGS+L F+ G F VGPES GAHPGP CYRKGG + VTDANLVLG ++P  FP IFGP E
Sbjct: 361  GGGSRLFFRSGLFVVGPESAGAHPGPACYRKGGPVTVTDANLVLGRLLPASFPCIFGPGE 420

Query: 421  DQPLDIEATRGEFEKLATEINSYRKNQDPSSKPMTIEEIALGFVNVANETMCRPIRQLTE 480
            DQPL  EA+R   E +A E+NS+  N    +  +++EE+A+GFV VANE MCRPIR LT+
Sbjct: 421  DQPLSPEASRKALEAVAMEVNSFLTNGPCPASQLSLEEVAMGFVRVANEAMCRPIRALTQ 480

Query: 481  MKGHETKNHALACFGGAGPQHACAIARLLGMKEIFIHRFCGILSAYGMGLADVVEEEQEP 540
             +GH+   H LACFGGAG QHACAIAR LGM  + IHR  G+LSA G+ LADVV E QEP
Sbjct: 481  ARGHDPSAHVLACFGGAGGQHACAIARALGMDTVHIHRHSGLLSALGLALADVVHEAQEP 540

Query: 541  YSAVYCSKSVQEVSRREASLLKQVKHKLRSQGFREGSINTETYLNLRYDGTDTAIMVKSQ 600
             S  Y  ++  ++ +R + L +Q    L+ QGF    I+TE++L+LRY GTD A+MV + 
Sbjct: 541  CSLSYTPETFAQLDQRLSRLEEQCVDALQVQGFPRSQISTESFLHLRYQGTDCALMVSAH 600

Query: 601  R----VDNGIEFDFAAEFEKLFQQEYGFKLQNRNILICDIRVRGVGVTNVLKPRAFEGLS 660
            +      +    DF A F + + +E+GF +  R +++ D+RVRG G + +      +  +
Sbjct: 601  QHPATACSPRAGDFGAAFVERYMREFGFIIPERPVVVDDVRVRGTGRSGLQLEDTPKIQT 660

Query: 661  GDPKIEGHYRVYFGNGWQDTPLFKLDNLGFGYIIPGPAIIMNGNSTVIVEPSCKATVTKY 720
            G P +E   + YF  G+Q+TP++ L  LG+G+ + GP +I++ NST++VEP C+A VT  
Sbjct: 661  GPPHVEKVTQCYFEGGYQETPVYLLGELGYGHQLQGPCLIIDNNSTILVEPGCQAEVTDT 720

Query: 721  GNIKIEIDSTFCTKKVSEKVADVVQLSIFNHQFMGIAEQMGRTLQRTSISTNIKERLDFS 780
            G+I+I + +      +++   D +QLSIF+H+FM IAEQMGR LQRT+ISTNIKERLDFS
Sbjct: 721  GDIRISVGAE--GPSMADTRLDPIQLSIFSHRFMSIAEQMGRILQRTAISTNIKERLDFS 780

Query: 781  CALFGPDGGLVANAPHVPVHLGAMSSTVRWQIDFWGDNLNEGDVLVTNHPCAGGSHLPDI 840
            CALFGPDGGLV+NAPH+PVHLGAM  TV++QI   G +L+ GDVL++NHP AGGSHLPD+
Sbjct: 781  CALFGPDGGLVSNAPHIPVHLGAMQETVQFQIQHLGADLHPGDVLLSNHPSAGGSHLPDL 840

Query: 841  TVITPVFDNGKL--IFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVEKGIF 900
            TVITPVF  G+   +F+VASRGHHA+IGGITPGSMPP S ++ +EGA   +FKLV+ G+F
Sbjct: 841  TVITPVFWPGQTRPVFYVASRGHHADIGGITPGSMPPHSTTLQQEGAVFLSFKLVQGGVF 900

Query: 901  QEEGINKLLQFPSSDEGVIPGTRRLQDNLSDLHAQVAANHRGISLIKELIVQYGLNIVQA 960
            QEE + + L+ P    G   GTR L DNLSDL AQVAAN +GI L+ ELI QYGL++VQA
Sbjct: 901  QEEAVTEALRAPGKISG-CSGTRNLHDNLSDLRAQVAANQKGIQLVGELIGQYGLDVVQA 960

Query: 961  YMTYVQLNAEEAVREMLKSVASRVSSNSAKYVEGGSIAIEEEDYMDDGSAIHLKLTIDPH 1020
            YM ++Q NAE AVR+ML++        +++   G  + +  ED+MDDGS I L++ I+  
Sbjct: 961  YMGHIQANAELAVRDMLRAF------GTSRQARGLPLEVSAEDHMDDGSPICLRVQINLS 1020

Query: 1021 KGEANFDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPPGS 1080
            +G A FDF+G+  EV+GN NAP A+T +A+IYCLRC+V  DIPLNQGCLAPV++ IP GS
Sbjct: 1021 QGSAVFDFTGSGSEVFGNLNAPRAITLSALIYCLRCLVGRDIPLNQGCLAPVRVIIPKGS 1080

Query: 1081 FLSPSEKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSG 1140
             L PS +AA+VGGNVLTSQR+ DVIL AF AC+ SQGCMNN+T G+   GYYET+ GG+G
Sbjct: 1081 ILDPSPEAAVVGGNVLTSQRVVDVILGAFGACSASQGCMNNVTLGNARMGYYETVAGGAG 1140

Query: 1141 AGPSWHGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGSGVYKGGDGLVREIE 1200
            AGP WHG SGV  HMTNTR+TDPEI E RYPV+L  F LR  SGG G ++GGDG+VRE+ 
Sbjct: 1141 AGPGWHGRSGVHSHMTNTRITDPEILESRYPVILRRFELRPGSGGRGRFRGGDGVVRELV 1200

Query: 1201 FKQPVVVSILSERRVHAPRGLKGGKDGARGANFLVRKDGRRVYLGGKNTITVKAGEILQI 1260
            F++  ++S+L+ERR   P GL GG+ GARG N L+RKDGR V LGGK ++TV  G++  +
Sbjct: 1201 FREEALLSVLTERRAFQPYGLHGGEPGARGLNLLIRKDGRTVNLGGKTSVTVYPGDVFCL 1254

Query: 1261 LTPGGGGWG 1264
             TPGGGG+G
Sbjct: 1261 HTPGGGGYG 1254

BLAST of Cucsat.G5649 vs. ExPASy Swiss-Prot
Match: Q75WB5 (5-oxoprolinase OS=Bos taurus OX=9913 GN=OPLAH PE=1 SV=1)

HSP 1 Score: 1414.1 bits (3659), Expect = 0.0e+00
Identity = 717/1264 (56.72%), Postives = 903/1264 (71.44%), Query Frame = 0

Query: 6    EEKLRFCIDRGGTFTDVYAEIPGRPDGKVFKLLSVDPSNYDDAPVEGIRRILEEYTGKKI 65
            E +  F IDRGGTFTDV+A+ PG    +V KLLS DP+NY DAP EGIRRILE+  G  +
Sbjct: 5    EGRFHFAIDRGGTFTDVFAQCPG-GHVRVLKLLSEDPANYVDAPTEGIRRILEQEGGVLL 64

Query: 66   PRTSKIPTQNIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDIFDLTV 125
            PR   + T  I  IRMGTTVATNALLE++GER+AL VT+GFRDLL +  QAR  +FDL V
Sbjct: 65   PRDRPLDTSRIASIRMGTTVATNALLEQQGERVALLVTRGFRDLLHVCTQARAXLFDLAV 124

Query: 126  SKPSNLYEDVVEVDERVELIHSKGDGNQDSSTYVEGVSGELIRIVKTLNEEALKPLLNDL 185
              P  LYE+V+EVDERV L      G   + T V+G +G+L+ + + ++   L+  L  L
Sbjct: 125  PMPETLYEEVLEVDERVVLYR----GXPGAGTPVKGCTGDLLEVQQPVDLGGLRWKLEGL 184

Query: 186  LQRGIGCLAVVLMHSYTYPQHELALEKLALSMGFKHVSLSSALTPMVRAVPRGLTASVDA 245
            L RGI  LAVVLMHSYT+ QHE  +  LA  +GF HVSLSS   PMVR VPRG TA  DA
Sbjct: 185  LSRGIRSLAVVLMHSYTWAQHEQQVGALARELGFTHVSLSSEAMPMVRIVPRGHTACADA 244

Query: 246  YLTPVIKEYLSGFMSKFDESSGKVNVLFMQSDGGLAPESRFSGHKAVLSGPAGGVVGYSQ 305
            YLTP I+ Y+ GF   F      V VLFM+SDGGLAP   FSG +AVLSGPAGGVVGYS 
Sbjct: 245  YLTPTIQRYVQGFRRGFQGQLKDVQVLFMRSDGGLAPMDSFSGSRAVLSGPAGGVVGYSA 304

Query: 306  TLFELETTKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVAAGGGSK 365
            T + +E  +P+IGFDMGGTSTDVSRYAG +E V E   AG  +QAPQLDINTVAAGGGS+
Sbjct: 305  TTYRVEGGQPVIGFDMGGTSTDVSRYAGEFEHVFEASTAGVTLQAPQLDINTVAAGGGSR 364

Query: 366  LKFQFGAFRVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPNEDQPLD 425
            L F+ G F VGPES GAHPGP CYRKGG + VTDANLVLG ++P  FP IFGP EDQPL 
Sbjct: 365  LFFRSGLFVVGPESAGAHPGPACYRKGGPVTVTDANLVLGRLLPASFPCIFGPGEDQPLS 424

Query: 426  IEATRGEFEKLATEINSYRKNQDPSSKPMTIEEIALGFVNVANETMCRPIRQLTEMKGHE 485
             EA+R   E +ATE+NS+  N    + P+++EE+A+GFV VANE MCRPIR LT+ +GH+
Sbjct: 425  PEASRKALEAVATEVNSFLTNGPCPASPLSLEEVAMGFVRVANEAMCRPIRALTQARGHD 484

Query: 486  TKNHALACFGGAGPQHACAIARLLGMKEIFIHRFCGILSAYGMGLADVVEEEQEPYSAVY 545
               H LACFGGAG QHACAIAR LGM  + IHR  G+LSA G+ LADVV E QEP S  Y
Sbjct: 485  PSAHVLACFGGAGGQHACAIARALGMDTVHIHRHSGLLSALGLALADVVHEAQEPCSLPY 544

Query: 546  CSKSVQEVSRREASLLKQVKHKLRSQGFREGSINTETYLNLRYDGTDTAIMVKSQRVDNG 605
              ++  ++ +R   L +Q    LR+QGF    I+TE++L+LRY GTD A+MV + +    
Sbjct: 545  APETFAQLDQRLGRLEEQCVEALRAQGFPRSQISTESFLHLRYQGTDCALMVSAHQHPAS 604

Query: 606  IEF----DFAAEFEKLFQQEYGFKLQNRNILICDIRVRGVGVTNVLKPRAFEGLSGDPKI 665
                   DF A F + + +E+GF +  R +++ D+RVRG G +++      +  SG P++
Sbjct: 605  ARSPRAGDFGAAFVERYMREFGFIIPERPVVVDDVRVRGTGSSSLRLEDVPKAHSGPPRV 664

Query: 666  EGHYRVYFGNGWQDTPLFKLDNLGFGYIIPGPAIIMNGNSTVIVEPSCKATVTKYGNIKI 725
            +   + YF  G+Q+TP++ L  LG G+ + GP +I++ NST++VEP C+A VT+ G+I+I
Sbjct: 665  DKMTQCYFEGGYQETPVYLLGELGCGHKLQGPCLIIDSNSTILVEPGCQAEVTETGDIRI 724

Query: 726  EIDSTFCTKKVSEKVADVVQLSIFNHQFMGIAEQMGRTLQRTSISTNIKERLDFSCALFG 785
             + +   T  V     D + L+IF+H+FM IAEQMGR LQRT+ISTNIKERLDFSCALFG
Sbjct: 725  SVGAE--TASVVGTQLDPIHLTIFSHRFMSIAEQMGRILQRTAISTNIKERLDFSCALFG 784

Query: 786  PDGGLVANAPHVPVHLGAMSSTVRWQIDFWGDNLNEGDVLVTNHPCAGGSHLPDITVITP 845
            PDGGLV+N PH+PVHLGAM  TV++QI   G +L+ GDVL++NHP AGGSHLPD+TVITP
Sbjct: 785  PDGGLVSNVPHIPVHLGAMQETVQFQIQQLGADLHPGDVLLSNHPSAGGSHLPDLTVITP 844

Query: 846  VFDNGKL--IFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVEKGIFQEEGI 905
            VF  G+   +F+VASRGHHA+IGGITPGSMPP S S+ +EGA   +FKLV  G+FQEE +
Sbjct: 845  VFWPGQTRPVFYVASRGHHADIGGITPGSMPPHSTSLQQEGAVFLSFKLVHGGVFQEEAV 904

Query: 906  NKLLQFPSSDEGVIPGTRRLQDNLSDLHAQVAANHRGISLIKELIVQYGLNIVQAYMTYV 965
             + L+ P    G   GTR L DNLSDL AQVAAN +GI L+ ELI QYGL++VQAYM ++
Sbjct: 905  TEALRAPGKIPG-CSGTRNLHDNLSDLRAQVAANQKGIQLVGELIGQYGLDVVQAYMGHI 964

Query: 966  QLNAEEAVREMLKSVASRVSSNSAKYVEGGSIAIEEEDYMDDGSAIHLKLTIDPHKGEAN 1025
            Q NAE AVR+ML++        +A+   G  + +  ED+MDDGS I L++ I+  +G A 
Sbjct: 965  QANAELAVRDMLRAF------GTARQARGLPLEVSAEDHMDDGSPIRLRVQINMSQGSAV 1024

Query: 1026 FDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPPGSFLSPS 1085
            FDFSG+ PEV+GN NAP A+T +A+IYCLRC+V  DIPLNQGCLAPV++ IP GS L PS
Sbjct: 1025 FDFSGSGPEVFGNLNAPRAITLSALIYCLRCLVGRDIPLNQGCLAPVRVVIPKGSILDPS 1084

Query: 1086 EKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGPSW 1145
              AA+VGGNVLTSQR+ DVIL AF ACA SQGCMNN+T G+   GYYET+ GG+GAGP W
Sbjct: 1085 PDAAVVGGNVLTSQRVVDVILGAFGACAASQGCMNNVTLGNAHMGYYETVAGGAGAGPGW 1144

Query: 1146 HGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGSGVYKGGDGLVREIEFKQPV 1205
            HG SGV  HMTNTR+TDPEI E RYPV+L  F LR  SGG G ++GGDG++RE+ F++  
Sbjct: 1145 HGRSGVHSHMTNTRITDPEILESRYPVILRRFELRLGSGGRGRFRGGDGIIRELLFREEA 1204

Query: 1206 VVSILSERRVHAPRGLKGGKDGARGANFLVRKDGRRVYLGGKNTITVKAGEILQILTPGG 1264
            ++S+L+ERR   P GL GG+ GARG N L+RKDGR V LGGK ++ V  G++  + TPGG
Sbjct: 1205 LLSVLTERRAFQPYGLMGGEPGARGLNLLIRKDGRTVNLGGKTSVPVYPGDVFCLHTPGG 1254

BLAST of Cucsat.G5649 vs. NCBI nr
Match: XP_031742450.1 (5-oxoprolinase isoform X2 [Cucumis sativus] >XP_031742451.1 5-oxoprolinase isoform X2 [Cucumis sativus])

HSP 1 Score: 2531 bits (6561), Expect = 0.0
Identity = 1263/1265 (99.84%), Postives = 1264/1265 (99.92%), Query Frame = 0

Query: 1    MGSNNEEKLRFCIDRGGTFTDVYAEIPGRPDGKVFKLLSVDPSNYDDAPVEGIRRILEEY 60
            MGSNNEEKLRFCIDRGGTFTDVYAEIPGRPDGKVFKLLSVDPSNYDDAPVEGIRRILEEY
Sbjct: 1    MGSNNEEKLRFCIDRGGTFTDVYAEIPGRPDGKVFKLLSVDPSNYDDAPVEGIRRILEEY 60

Query: 61   TGKKIPRTSKIPTQNIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI 120
            TGKKIPRTSKIPTQNIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI
Sbjct: 61   TGKKIPRTSKIPTQNIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI 120

Query: 121  FDLTVSKPSNLYEDVVEVDERVELIHSKGDGNQDSSTYVEGVSGELIRIVKTLNEEALKP 180
            FDLTVSKPSNLYEDVVEVDERVELIH KGDGNQDSSTYVEGVSGELIRIVKTLNEEALKP
Sbjct: 121  FDLTVSKPSNLYEDVVEVDERVELIHGKGDGNQDSSTYVEGVSGELIRIVKTLNEEALKP 180

Query: 181  LLNDLLQRGIGCLAVVLMHSYTYPQHELALEKLALSMGFKHVSLSSALTPMVRAVPRGLT 240
            LLNDLLQRGIGCLAVVLMHSYTYPQHELALEKLALSMGFKHVSLSSALTPMVRAVPRGLT
Sbjct: 181  LLNDLLQRGIGCLAVVLMHSYTYPQHELALEKLALSMGFKHVSLSSALTPMVRAVPRGLT 240

Query: 241  ASVDAYLTPVIKEYLSGFMSKFDESSGKVNVLFMQSDGGLAPESRFSGHKAVLSGPAGGV 300
            ASVDAYLTPVIKEYLSGFMSKFDESSGKVNVLFMQSDGGLAPESRFSGHKAVLSGPAGGV
Sbjct: 241  ASVDAYLTPVIKEYLSGFMSKFDESSGKVNVLFMQSDGGLAPESRFSGHKAVLSGPAGGV 300

Query: 301  VGYSQTLFELETTKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVAA 360
            VGYSQTLFELETTKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVAA
Sbjct: 301  VGYSQTLFELETTKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVAA 360

Query: 361  GGGSKLKFQFGAFRVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPNE 420
            GGGSKLKFQFGAFRVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPNE
Sbjct: 361  GGGSKLKFQFGAFRVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPNE 420

Query: 421  DQPLDIEATRGEFEKLATEINSYRKNQDPSSKPMTIEEIALGFVNVANETMCRPIRQLTE 480
            DQPLDIEATRGEFEKLATEINSYRKNQDPSSKPMTIEEIALGFVNVANETMCRPIRQLTE
Sbjct: 421  DQPLDIEATRGEFEKLATEINSYRKNQDPSSKPMTIEEIALGFVNVANETMCRPIRQLTE 480

Query: 481  MKGHETKNHALACFGGAGPQHACAIARLLGMKEIFIHRFCGILSAYGMGLADVVEEEQEP 540
            MKGHETKNHALACFGGAGPQHACAIARLLGMKEIFIHRFCGILSAYGMGLADVVEEEQEP
Sbjct: 481  MKGHETKNHALACFGGAGPQHACAIARLLGMKEIFIHRFCGILSAYGMGLADVVEEEQEP 540

Query: 541  YSAVYCSKSVQEVSRREASLLKQVKHKLRSQGFREGSINTETYLNLRYDGTDTAIMVKSQ 600
            YSAVYCSKSVQEVSRREASLLKQVKHKLRSQGFREGSINTETYLNLRYDGTDTAIMVKSQ
Sbjct: 541  YSAVYCSKSVQEVSRREASLLKQVKHKLRSQGFREGSINTETYLNLRYDGTDTAIMVKSQ 600

Query: 601  RVDNGIEFDFAAEFEKLFQQEYGFKLQNRNILICDIRVRGVGVTNVLKPRAFEGLSGDPK 660
            RVDNG+EFDFAAEFEKLFQQEYGFKLQNRNILICDIRVRGVGVTNVLKPRAFEGLSGDPK
Sbjct: 601  RVDNGVEFDFAAEFEKLFQQEYGFKLQNRNILICDIRVRGVGVTNVLKPRAFEGLSGDPK 660

Query: 661  IEGHYRVYFGNGWQDTPLFKLDNLGFGYIIPGPAIIMNGNSTVIVEPSCKATVTKYGNIK 720
            IEGHYRVYFGNGWQDTPLFKLDNLGFGYIIPGPAIIMNGNSTVIVEPSCKATVTKYGNIK
Sbjct: 661  IEGHYRVYFGNGWQDTPLFKLDNLGFGYIIPGPAIIMNGNSTVIVEPSCKATVTKYGNIK 720

Query: 721  IEIDSTFCTKKVSEKVADVVQLSIFNHQFMGIAEQMGRTLQRTSISTNIKERLDFSCALF 780
            IEIDSTFCTKKVSEKVADVVQLSIFNHQFMGIAEQMGRTLQRTSISTNIKERLDFSCALF
Sbjct: 721  IEIDSTFCTKKVSEKVADVVQLSIFNHQFMGIAEQMGRTLQRTSISTNIKERLDFSCALF 780

Query: 781  GPDGGLVANAPHVPVHLGAMSSTVRWQIDFWGDNLNEGDVLVTNHPCAGGSHLPDITVIT 840
            GPDGGLVANAPHVPVHLGAMSSTVRWQIDFWGDNLNEGDVLVTNHPCAGGSHLPDITVIT
Sbjct: 781  GPDGGLVANAPHVPVHLGAMSSTVRWQIDFWGDNLNEGDVLVTNHPCAGGSHLPDITVIT 840

Query: 841  PVFDNGKLIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVEKGIFQEEGIN 900
            PVFDNGKLIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVEKGIFQEEGIN
Sbjct: 841  PVFDNGKLIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVEKGIFQEEGIN 900

Query: 901  KLLQFPSSDEGVIPGTRRLQDNLSDLHAQVAANHRGISLIKELIVQYGLNIVQAYMTYVQ 960
            KLLQFPSSDEGVIPGTRRLQDNLSDLHAQVAANHRGISLIKELIVQYGLNIVQAYMTYVQ
Sbjct: 901  KLLQFPSSDEGVIPGTRRLQDNLSDLHAQVAANHRGISLIKELIVQYGLNIVQAYMTYVQ 960

Query: 961  LNAEEAVREMLKSVASRVSSNSAKYVEGGSIAIEEEDYMDDGSAIHLKLTIDPHKGEANF 1020
            LNAEEAVREMLKSVASRVSSNSAKYVEGGSIAIEEEDYMDDGSAIHLKLTIDPHKGEANF
Sbjct: 961  LNAEEAVREMLKSVASRVSSNSAKYVEGGSIAIEEEDYMDDGSAIHLKLTIDPHKGEANF 1020

Query: 1021 DFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPPGSFLSPSE 1080
            DFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPPGSFLSPSE
Sbjct: 1021 DFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPPGSFLSPSE 1080

Query: 1081 KAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGPSWH 1140
            KAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGPSWH
Sbjct: 1081 KAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGPSWH 1140

Query: 1141 GTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGSGVYKGGDGLVREIEFKQPVV 1200
            GTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGSGVYKGGDGLVREIEFKQPVV
Sbjct: 1141 GTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGSGVYKGGDGLVREIEFKQPVV 1200

Query: 1201 VSILSERRVHAPRGLKGGKDGARGANFLVRKDGRRVYLGGKNTITVKAGEILQILTPGGG 1260
            VSILSERRVHAPRGLKGGKDGARGANFLVRKDGRRVYLGGKNTITVKAGEILQILTPGGG
Sbjct: 1201 VSILSERRVHAPRGLKGGKDGARGANFLVRKDGRRVYLGGKNTITVKAGEILQILTPGGG 1260

Query: 1261 GWGCA 1265
            GWGCA
Sbjct: 1261 GWGCA 1265

BLAST of Cucsat.G5649 vs. NCBI nr
Match: XP_004138530.2 (5-oxoprolinase isoform X1 [Cucumis sativus] >KGN45606.2 hypothetical protein Csa_004950 [Cucumis sativus])

HSP 1 Score: 2531 bits (6561), Expect = 0.0
Identity = 1263/1265 (99.84%), Postives = 1264/1265 (99.92%), Query Frame = 0

Query: 1    MGSNNEEKLRFCIDRGGTFTDVYAEIPGRPDGKVFKLLSVDPSNYDDAPVEGIRRILEEY 60
            MGSNNEEKLRFCIDRGGTFTDVYAEIPGRPDGKVFKLLSVDPSNYDDAPVEGIRRILEEY
Sbjct: 76   MGSNNEEKLRFCIDRGGTFTDVYAEIPGRPDGKVFKLLSVDPSNYDDAPVEGIRRILEEY 135

Query: 61   TGKKIPRTSKIPTQNIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI 120
            TGKKIPRTSKIPTQNIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI
Sbjct: 136  TGKKIPRTSKIPTQNIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI 195

Query: 121  FDLTVSKPSNLYEDVVEVDERVELIHSKGDGNQDSSTYVEGVSGELIRIVKTLNEEALKP 180
            FDLTVSKPSNLYEDVVEVDERVELIH KGDGNQDSSTYVEGVSGELIRIVKTLNEEALKP
Sbjct: 196  FDLTVSKPSNLYEDVVEVDERVELIHGKGDGNQDSSTYVEGVSGELIRIVKTLNEEALKP 255

Query: 181  LLNDLLQRGIGCLAVVLMHSYTYPQHELALEKLALSMGFKHVSLSSALTPMVRAVPRGLT 240
            LLNDLLQRGIGCLAVVLMHSYTYPQHELALEKLALSMGFKHVSLSSALTPMVRAVPRGLT
Sbjct: 256  LLNDLLQRGIGCLAVVLMHSYTYPQHELALEKLALSMGFKHVSLSSALTPMVRAVPRGLT 315

Query: 241  ASVDAYLTPVIKEYLSGFMSKFDESSGKVNVLFMQSDGGLAPESRFSGHKAVLSGPAGGV 300
            ASVDAYLTPVIKEYLSGFMSKFDESSGKVNVLFMQSDGGLAPESRFSGHKAVLSGPAGGV
Sbjct: 316  ASVDAYLTPVIKEYLSGFMSKFDESSGKVNVLFMQSDGGLAPESRFSGHKAVLSGPAGGV 375

Query: 301  VGYSQTLFELETTKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVAA 360
            VGYSQTLFELETTKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVAA
Sbjct: 376  VGYSQTLFELETTKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVAA 435

Query: 361  GGGSKLKFQFGAFRVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPNE 420
            GGGSKLKFQFGAFRVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPNE
Sbjct: 436  GGGSKLKFQFGAFRVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPNE 495

Query: 421  DQPLDIEATRGEFEKLATEINSYRKNQDPSSKPMTIEEIALGFVNVANETMCRPIRQLTE 480
            DQPLDIEATRGEFEKLATEINSYRKNQDPSSKPMTIEEIALGFVNVANETMCRPIRQLTE
Sbjct: 496  DQPLDIEATRGEFEKLATEINSYRKNQDPSSKPMTIEEIALGFVNVANETMCRPIRQLTE 555

Query: 481  MKGHETKNHALACFGGAGPQHACAIARLLGMKEIFIHRFCGILSAYGMGLADVVEEEQEP 540
            MKGHETKNHALACFGGAGPQHACAIARLLGMKEIFIHRFCGILSAYGMGLADVVEEEQEP
Sbjct: 556  MKGHETKNHALACFGGAGPQHACAIARLLGMKEIFIHRFCGILSAYGMGLADVVEEEQEP 615

Query: 541  YSAVYCSKSVQEVSRREASLLKQVKHKLRSQGFREGSINTETYLNLRYDGTDTAIMVKSQ 600
            YSAVYCSKSVQEVSRREASLLKQVKHKLRSQGFREGSINTETYLNLRYDGTDTAIMVKSQ
Sbjct: 616  YSAVYCSKSVQEVSRREASLLKQVKHKLRSQGFREGSINTETYLNLRYDGTDTAIMVKSQ 675

Query: 601  RVDNGIEFDFAAEFEKLFQQEYGFKLQNRNILICDIRVRGVGVTNVLKPRAFEGLSGDPK 660
            RVDNG+EFDFAAEFEKLFQQEYGFKLQNRNILICDIRVRGVGVTNVLKPRAFEGLSGDPK
Sbjct: 676  RVDNGVEFDFAAEFEKLFQQEYGFKLQNRNILICDIRVRGVGVTNVLKPRAFEGLSGDPK 735

Query: 661  IEGHYRVYFGNGWQDTPLFKLDNLGFGYIIPGPAIIMNGNSTVIVEPSCKATVTKYGNIK 720
            IEGHYRVYFGNGWQDTPLFKLDNLGFGYIIPGPAIIMNGNSTVIVEPSCKATVTKYGNIK
Sbjct: 736  IEGHYRVYFGNGWQDTPLFKLDNLGFGYIIPGPAIIMNGNSTVIVEPSCKATVTKYGNIK 795

Query: 721  IEIDSTFCTKKVSEKVADVVQLSIFNHQFMGIAEQMGRTLQRTSISTNIKERLDFSCALF 780
            IEIDSTFCTKKVSEKVADVVQLSIFNHQFMGIAEQMGRTLQRTSISTNIKERLDFSCALF
Sbjct: 796  IEIDSTFCTKKVSEKVADVVQLSIFNHQFMGIAEQMGRTLQRTSISTNIKERLDFSCALF 855

Query: 781  GPDGGLVANAPHVPVHLGAMSSTVRWQIDFWGDNLNEGDVLVTNHPCAGGSHLPDITVIT 840
            GPDGGLVANAPHVPVHLGAMSSTVRWQIDFWGDNLNEGDVLVTNHPCAGGSHLPDITVIT
Sbjct: 856  GPDGGLVANAPHVPVHLGAMSSTVRWQIDFWGDNLNEGDVLVTNHPCAGGSHLPDITVIT 915

Query: 841  PVFDNGKLIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVEKGIFQEEGIN 900
            PVFDNGKLIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVEKGIFQEEGIN
Sbjct: 916  PVFDNGKLIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVEKGIFQEEGIN 975

Query: 901  KLLQFPSSDEGVIPGTRRLQDNLSDLHAQVAANHRGISLIKELIVQYGLNIVQAYMTYVQ 960
            KLLQFPSSDEGVIPGTRRLQDNLSDLHAQVAANHRGISLIKELIVQYGLNIVQAYMTYVQ
Sbjct: 976  KLLQFPSSDEGVIPGTRRLQDNLSDLHAQVAANHRGISLIKELIVQYGLNIVQAYMTYVQ 1035

Query: 961  LNAEEAVREMLKSVASRVSSNSAKYVEGGSIAIEEEDYMDDGSAIHLKLTIDPHKGEANF 1020
            LNAEEAVREMLKSVASRVSSNSAKYVEGGSIAIEEEDYMDDGSAIHLKLTIDPHKGEANF
Sbjct: 1036 LNAEEAVREMLKSVASRVSSNSAKYVEGGSIAIEEEDYMDDGSAIHLKLTIDPHKGEANF 1095

Query: 1021 DFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPPGSFLSPSE 1080
            DFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPPGSFLSPSE
Sbjct: 1096 DFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPPGSFLSPSE 1155

Query: 1081 KAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGPSWH 1140
            KAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGPSWH
Sbjct: 1156 KAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGPSWH 1215

Query: 1141 GTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGSGVYKGGDGLVREIEFKQPVV 1200
            GTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGSGVYKGGDGLVREIEFKQPVV
Sbjct: 1216 GTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGSGVYKGGDGLVREIEFKQPVV 1275

Query: 1201 VSILSERRVHAPRGLKGGKDGARGANFLVRKDGRRVYLGGKNTITVKAGEILQILTPGGG 1260
            VSILSERRVHAPRGLKGGKDGARGANFLVRKDGRRVYLGGKNTITVKAGEILQILTPGGG
Sbjct: 1276 VSILSERRVHAPRGLKGGKDGARGANFLVRKDGRRVYLGGKNTITVKAGEILQILTPGGG 1335

Query: 1261 GWGCA 1265
            GWGCA
Sbjct: 1336 GWGCA 1340

BLAST of Cucsat.G5649 vs. NCBI nr
Match: XP_008458316.1 (PREDICTED: 5-oxoprolinase [Cucumis melo] >TYK02972.1 5-oxoprolinase [Cucumis melo var. makuwa])

HSP 1 Score: 2484 bits (6439), Expect = 0.0
Identity = 1238/1265 (97.87%), Postives = 1251/1265 (98.89%), Query Frame = 0

Query: 1    MGSNNEEKLRFCIDRGGTFTDVYAEIPGRPDGKVFKLLSVDPSNYDDAPVEGIRRILEEY 60
            MGSNNEEKLRFCIDRGGTFTDVYAEIPGRPDGKV KLLSVDPSNYDDAPVEGIRRILEEY
Sbjct: 1    MGSNNEEKLRFCIDRGGTFTDVYAEIPGRPDGKVLKLLSVDPSNYDDAPVEGIRRILEEY 60

Query: 61   TGKKIPRTSKIPTQNIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI 120
            TGKKIPRTSKIPTQNIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI
Sbjct: 61   TGKKIPRTSKIPTQNIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI 120

Query: 121  FDLTVSKPSNLYEDVVEVDERVELIHSKGDGNQDSSTYVEGVSGELIRIVKTLNEEALKP 180
            FDLTVSKPSNLYEDVVEVDERVELIH KGDGNQDSSTYV+GVSGELIRIVKTLNEEALKP
Sbjct: 121  FDLTVSKPSNLYEDVVEVDERVELIHGKGDGNQDSSTYVKGVSGELIRIVKTLNEEALKP 180

Query: 181  LLNDLLQRGIGCLAVVLMHSYTYPQHELALEKLALSMGFKHVSLSSALTPMVRAVPRGLT 240
            LL DLLQRGIGCLAVVLMHSYTYPQHELALEKLALSMGFKHVSLSSALTPMVRAVPRGLT
Sbjct: 181  LLKDLLQRGIGCLAVVLMHSYTYPQHELALEKLALSMGFKHVSLSSALTPMVRAVPRGLT 240

Query: 241  ASVDAYLTPVIKEYLSGFMSKFDESSGKVNVLFMQSDGGLAPESRFSGHKAVLSGPAGGV 300
            ASVDAYLTPVIKEYLSGFMSKFD+SSGKVNVLFMQSDGGLAPESRFSGHKAVLSGPAGGV
Sbjct: 241  ASVDAYLTPVIKEYLSGFMSKFDKSSGKVNVLFMQSDGGLAPESRFSGHKAVLSGPAGGV 300

Query: 301  VGYSQTLFELETTKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVAA 360
            VGYSQTLFELETTKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVAA
Sbjct: 301  VGYSQTLFELETTKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVAA 360

Query: 361  GGGSKLKFQFGAFRVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPNE 420
            GGGSKLKFQFGAFRVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPNE
Sbjct: 361  GGGSKLKFQFGAFRVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPNE 420

Query: 421  DQPLDIEATRGEFEKLATEINSYRKNQDPSSKPMTIEEIALGFVNVANETMCRPIRQLTE 480
            DQPLDIEATRGEFEKLATEINSYRKNQDPSSKPMTIE+IALGFVNVANETMCRPIRQLTE
Sbjct: 421  DQPLDIEATRGEFEKLATEINSYRKNQDPSSKPMTIEQIALGFVNVANETMCRPIRQLTE 480

Query: 481  MKGHETKNHALACFGGAGPQHACAIARLLGMKEIFIHRFCGILSAYGMGLADVVEEEQEP 540
            MKGHETKNHALACFGGAGPQHACAIARLLGMKEIFIHRFCGILSAYGMGLADVVEEEQEP
Sbjct: 481  MKGHETKNHALACFGGAGPQHACAIARLLGMKEIFIHRFCGILSAYGMGLADVVEEEQEP 540

Query: 541  YSAVYCSKSVQEVSRREASLLKQVKHKLRSQGFREGSINTETYLNLRYDGTDTAIMVKSQ 600
            YSAVYCS+S  EVSRREASLLKQVKHKL+SQGFREGSI TETYLNLRYDGTDTAIMVK Q
Sbjct: 541  YSAVYCSESFLEVSRREASLLKQVKHKLQSQGFREGSIKTETYLNLRYDGTDTAIMVKGQ 600

Query: 601  RVDNGIEFDFAAEFEKLFQQEYGFKLQNRNILICDIRVRGVGVTNVLKPRAFEGLSGDPK 660
            R DNG+EFDFAAEFEKLFQQEYGFKLQNRNILICDIRVRGVGVTNVLKPRAFEGLSGDPK
Sbjct: 601  RADNGVEFDFAAEFEKLFQQEYGFKLQNRNILICDIRVRGVGVTNVLKPRAFEGLSGDPK 660

Query: 661  IEGHYRVYFGNGWQDTPLFKLDNLGFGYIIPGPAIIMNGNSTVIVEPSCKATVTKYGNIK 720
            IEGHYRVYFGNGWQDTPL KLDNLGFG+IIPGPAIIMNGNSTVIVEPSCKATVTKYGNIK
Sbjct: 661  IEGHYRVYFGNGWQDTPLLKLDNLGFGHIIPGPAIIMNGNSTVIVEPSCKATVTKYGNIK 720

Query: 721  IEIDSTFCTKKVSEKVADVVQLSIFNHQFMGIAEQMGRTLQRTSISTNIKERLDFSCALF 780
            IEIDSTFCT+ VSEKVADVVQLSIFNH+FMGIAEQMGRTLQRTSISTNIKERLDFSCALF
Sbjct: 721  IEIDSTFCTENVSEKVADVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCALF 780

Query: 781  GPDGGLVANAPHVPVHLGAMSSTVRWQIDFWGDNLNEGDVLVTNHPCAGGSHLPDITVIT 840
            GPDGGLVANAPHVPVHLGAMSSTVRWQID+WGDNLNEGDVLVTNHPCAGGSHLPDITVIT
Sbjct: 781  GPDGGLVANAPHVPVHLGAMSSTVRWQIDYWGDNLNEGDVLVTNHPCAGGSHLPDITVIT 840

Query: 841  PVFDNGKLIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVEKGIFQEEGIN 900
            PVFDNGKLIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVEKGIFQEEGI 
Sbjct: 841  PVFDNGKLIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVEKGIFQEEGII 900

Query: 901  KLLQFPSSDEGVIPGTRRLQDNLSDLHAQVAANHRGISLIKELIVQYGLNIVQAYMTYVQ 960
            KLLQFPSSDEGVIPGTRRLQDNLSDLHAQVAANHRGISLIKELIVQYGLNIVQAYMTYVQ
Sbjct: 901  KLLQFPSSDEGVIPGTRRLQDNLSDLHAQVAANHRGISLIKELIVQYGLNIVQAYMTYVQ 960

Query: 961  LNAEEAVREMLKSVASRVSSNSAKYVEGGSIAIEEEDYMDDGSAIHLKLTIDPHKGEANF 1020
            LNAEEAVREMLKSVASRVSSNSA+ VEGGSI IEEEDYMDDGSAIHLKLTIDP+KGEANF
Sbjct: 961  LNAEEAVREMLKSVASRVSSNSARSVEGGSIVIEEEDYMDDGSAIHLKLTIDPNKGEANF 1020

Query: 1021 DFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPPGSFLSPSE 1080
            DFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPPGSFLSPSE
Sbjct: 1021 DFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPPGSFLSPSE 1080

Query: 1081 KAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGPSWH 1140
            KAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGPSWH
Sbjct: 1081 KAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGPSWH 1140

Query: 1141 GTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGSGVYKGGDGLVREIEFKQPVV 1200
            GTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGSGVYKGGDGLVREIEFKQPVV
Sbjct: 1141 GTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGSGVYKGGDGLVREIEFKQPVV 1200

Query: 1201 VSILSERRVHAPRGLKGGKDGARGANFLVRKDGRRVYLGGKNTITVKAGEILQILTPGGG 1260
            VSILSERRVHAPRGLKGGKDGA GANFLVRKDGRRVYLGGKNT+TVKAGEILQILTPGGG
Sbjct: 1201 VSILSERRVHAPRGLKGGKDGAHGANFLVRKDGRRVYLGGKNTVTVKAGEILQILTPGGG 1260

Query: 1261 GWGCA 1265
            GWGCA
Sbjct: 1261 GWGCA 1265

BLAST of Cucsat.G5649 vs. NCBI nr
Match: XP_038907125.1 (5-oxoprolinase [Benincasa hispida] >XP_038907126.1 5-oxoprolinase [Benincasa hispida] >XP_038907127.1 5-oxoprolinase [Benincasa hispida])

HSP 1 Score: 2445 bits (6337), Expect = 0.0
Identity = 1215/1265 (96.05%), Postives = 1244/1265 (98.34%), Query Frame = 0

Query: 1    MGSNNEEKLRFCIDRGGTFTDVYAEIPGRPDGKVFKLLSVDPSNYDDAPVEGIRRILEEY 60
            MGSNNEEKLRFCIDRGGTFTDVYAEIPGRPDGKV KLLSVDPSNYDDAPVEGIRRILEEY
Sbjct: 1    MGSNNEEKLRFCIDRGGTFTDVYAEIPGRPDGKVIKLLSVDPSNYDDAPVEGIRRILEEY 60

Query: 61   TGKKIPRTSKIPTQNIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI 120
            T KKIPRTSKIPTQNIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI
Sbjct: 61   TSKKIPRTSKIPTQNIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI 120

Query: 121  FDLTVSKPSNLYEDVVEVDERVELIHSKGDGNQDSST-YVEGVSGELIRIVKTLNEEALK 180
            FDLTVSKPSNLYEDV+EVDERVELIH KGDGNQDSST YV+GVSGELIRIVKTLNEEALK
Sbjct: 121  FDLTVSKPSNLYEDVIEVDERVELIHGKGDGNQDSSTSYVKGVSGELIRIVKTLNEEALK 180

Query: 181  PLLNDLLQRGIGCLAVVLMHSYTYPQHELALEKLALSMGFKHVSLSSALTPMVRAVPRGL 240
            PLL DLLQR I CLAVVLMHSYTYP+HELALEKLALSMGFKHVSLSSALTPMVRAVPRGL
Sbjct: 181  PLLKDLLQRRISCLAVVLMHSYTYPEHELALEKLALSMGFKHVSLSSALTPMVRAVPRGL 240

Query: 241  TASVDAYLTPVIKEYLSGFMSKFDESSGKVNVLFMQSDGGLAPESRFSGHKAVLSGPAGG 300
            TASVDAYLTPVIKEYLSGFMSKFDESSGKVNVLFMQSDGGLAPE+RFSGHKAVLSGPAGG
Sbjct: 241  TASVDAYLTPVIKEYLSGFMSKFDESSGKVNVLFMQSDGGLAPENRFSGHKAVLSGPAGG 300

Query: 301  VVGYSQTLFELETTKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVA 360
            VVGYSQTLF+LETTKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVA
Sbjct: 301  VVGYSQTLFKLETTKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVA 360

Query: 361  AGGGSKLKFQFGAFRVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPN 420
            AGGGSKLKFQFGAFRVGPESVGAHPGPVCYRKGGELAVTDANLVLG VIP FFPSIFGPN
Sbjct: 361  AGGGSKLKFQFGAFRVGPESVGAHPGPVCYRKGGELAVTDANLVLGLVIPAFFPSIFGPN 420

Query: 421  EDQPLDIEATRGEFEKLATEINSYRKNQDPSSKPMTIEEIALGFVNVANETMCRPIRQLT 480
            EDQPLDIEATRG+FEKLATEINSYRKNQDPSSKPMTIEEIALGFVNVANETMCRPIRQLT
Sbjct: 421  EDQPLDIEATRGKFEKLATEINSYRKNQDPSSKPMTIEEIALGFVNVANETMCRPIRQLT 480

Query: 481  EMKGHETKNHALACFGGAGPQHACAIARLLGMKEIFIHRFCGILSAYGMGLADVVEEEQE 540
            EMKGHETKNHALACFGGAGPQHACAIARLLGMKEIFIHRFCGILSAYGMGLADVVEEEQE
Sbjct: 481  EMKGHETKNHALACFGGAGPQHACAIARLLGMKEIFIHRFCGILSAYGMGLADVVEEEQE 540

Query: 541  PYSAVYCSKSVQEVSRREASLLKQVKHKLRSQGFREGSINTETYLNLRYDGTDTAIMVKS 600
            PY AVYCS+SVQ+VS+REASLL+QVKHKL+ QGFREGSI TETYLNLRY+GTDTA+MVKS
Sbjct: 541  PYLAVYCSESVQDVSQREASLLEQVKHKLQGQGFREGSIKTETYLNLRYEGTDTAMMVKS 600

Query: 601  QRVDNGIEFDFAAEFEKLFQQEYGFKLQNRNILICDIRVRGVGVTNVLKPRAFEGLSGDP 660
            Q+ DNG+EFDFAAEFEKLFQQEYGFKLQNRNILICDIRVRG+GVTNVLKPRAFEGL+GDP
Sbjct: 601  QKADNGVEFDFAAEFEKLFQQEYGFKLQNRNILICDIRVRGIGVTNVLKPRAFEGLAGDP 660

Query: 661  KIEGHYRVYFGNGWQDTPLFKLDNLGFGYIIPGPAIIMNGNSTVIVEPSCKATVTKYGNI 720
            KIEGHYRVYFGNGWQDTPLFKLDNLGFG+IIPGPAIIMNGNSTVIVEPSCKATVTKYGNI
Sbjct: 661  KIEGHYRVYFGNGWQDTPLFKLDNLGFGHIIPGPAIIMNGNSTVIVEPSCKATVTKYGNI 720

Query: 721  KIEIDSTFCTKKVSEKVADVVQLSIFNHQFMGIAEQMGRTLQRTSISTNIKERLDFSCAL 780
            KIE+DSTFCT+KVSEKVADVVQLSIFNH+FMGIAEQMGRTLQRTSISTNIKERLDFSCAL
Sbjct: 721  KIELDSTFCTEKVSEKVADVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCAL 780

Query: 781  FGPDGGLVANAPHVPVHLGAMSSTVRWQIDFWGDNLNEGDVLVTNHPCAGGSHLPDITVI 840
            FGPDGGLVANAPHVPVHLGAMSSTVRWQI++WGDNLNEGDVLVTNHPCAGGSHLPDITVI
Sbjct: 781  FGPDGGLVANAPHVPVHLGAMSSTVRWQIEYWGDNLNEGDVLVTNHPCAGGSHLPDITVI 840

Query: 841  TPVFDNGKLIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVEKGIFQEEGI 900
            TPVFDNGKLIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVEKGIFQEEGI
Sbjct: 841  TPVFDNGKLIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVEKGIFQEEGI 900

Query: 901  NKLLQFPSSDEGVIPGTRRLQDNLSDLHAQVAANHRGISLIKELIVQYGLNIVQAYMTYV 960
             KLLQFPSSDEGVIPGTRRLQDNLSDLHAQVAANHRGISLIKELI QYGLNIVQAYMTYV
Sbjct: 901  IKLLQFPSSDEGVIPGTRRLQDNLSDLHAQVAANHRGISLIKELIAQYGLNIVQAYMTYV 960

Query: 961  QLNAEEAVREMLKSVASRVSSNSAKYVEGGSIAIEEEDYMDDGSAIHLKLTIDPHKGEAN 1020
            QLNAEEAVREMLKSVASRVSSNSA+ V+G SI IEEEDYMDDGS IHLKLTIDPHKGEAN
Sbjct: 961  QLNAEEAVREMLKSVASRVSSNSARSVQGSSITIEEEDYMDDGSVIHLKLTIDPHKGEAN 1020

Query: 1021 FDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPPGSFLSPS 1080
            FDFSGTSPEVYGNWN PEAVTAAAVIYCLRC+V+VDIPLNQGCLAPVKIYIPPGSFLSPS
Sbjct: 1021 FDFSGTSPEVYGNWNTPEAVTAAAVIYCLRCLVNVDIPLNQGCLAPVKIYIPPGSFLSPS 1080

Query: 1081 EKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGPSW 1140
            EKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGPSW
Sbjct: 1081 EKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGPSW 1140

Query: 1141 HGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGSGVYKGGDGLVREIEFKQPV 1200
            HGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGSGVYKGGDGLVREIEFKQPV
Sbjct: 1141 HGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGSGVYKGGDGLVREIEFKQPV 1200

Query: 1201 VVSILSERRVHAPRGLKGGKDGARGANFLVRKDGRRVYLGGKNTITVKAGEILQILTPGG 1260
            VVSILSERRV+APRGLKGGK GARGANFLVRKDGRRVYLGGKNT+TVKAGEILQILTPGG
Sbjct: 1201 VVSILSERRVYAPRGLKGGKGGARGANFLVRKDGRRVYLGGKNTVTVKAGEILQILTPGG 1260

Query: 1261 GGWGC 1264
            GGWGC
Sbjct: 1261 GGWGC 1265

BLAST of Cucsat.G5649 vs. NCBI nr
Match: XP_022924406.1 (5-oxoprolinase [Cucurbita moschata])

HSP 1 Score: 2410 bits (6246), Expect = 0.0
Identity = 1197/1265 (94.62%), Postives = 1229/1265 (97.15%), Query Frame = 0

Query: 1    MGSNNEEKLRFCIDRGGTFTDVYAEIPGRPDGKVFKLLSVDPSNYDDAPVEGIRRILEEY 60
            MGS+NE+KLRFCIDRGGTFTDVYAEIPGRPDGKV KLLSVDPSNYDDAPVEGIRRILEEY
Sbjct: 1    MGSSNEDKLRFCIDRGGTFTDVYAEIPGRPDGKVIKLLSVDPSNYDDAPVEGIRRILEEY 60

Query: 61   TGKKIPRTSKIPTQNIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI 120
             GKKIPRTSKIPTQNIEWIRMGTTVATNALLER+GERIALCVTKGFRDLLQIGNQARP+I
Sbjct: 61   GGKKIPRTSKIPTQNIEWIRMGTTVATNALLERRGERIALCVTKGFRDLLQIGNQARPNI 120

Query: 121  FDLTVSKPSNLYEDVVEVDERVELIHSKGDGNQDSST-YVEGVSGELIRIVKTLNEEALK 180
            FDLTVSKPSNLYEDV+EVDERVEL+H KGD NQDSST YV+GVSGELI IVKTLNEEALK
Sbjct: 121  FDLTVSKPSNLYEDVIEVDERVELVHGKGDDNQDSSTSYVKGVSGELIHIVKTLNEEALK 180

Query: 181  PLLNDLLQRGIGCLAVVLMHSYTYPQHELALEKLALSMGFKHVSLSSALTPMVRAVPRGL 240
            PLL DLLQRGI CLAVVLMHSYTYPQHELALEKLA SMGFKHVSLSSALTPMVRAVPRGL
Sbjct: 181  PLLKDLLQRGISCLAVVLMHSYTYPQHELALEKLARSMGFKHVSLSSALTPMVRAVPRGL 240

Query: 241  TASVDAYLTPVIKEYLSGFMSKFDESSGKVNVLFMQSDGGLAPESRFSGHKAVLSGPAGG 300
            TASVDAYLTPVIKEYLSGFMSKFDESSGKVNVLFMQSDGGLAPE+RFSGHKAVLSGPAGG
Sbjct: 241  TASVDAYLTPVIKEYLSGFMSKFDESSGKVNVLFMQSDGGLAPENRFSGHKAVLSGPAGG 300

Query: 301  VVGYSQTLFELETTKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVA 360
            VVGYSQTLF+LET KPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVA
Sbjct: 301  VVGYSQTLFDLETRKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVA 360

Query: 361  AGGGSKLKFQFGAFRVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPN 420
            AGGGSKLKFQFGAFRVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPN
Sbjct: 361  AGGGSKLKFQFGAFRVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPN 420

Query: 421  EDQPLDIEATRGEFEKLATEINSYRKNQDPSSKPMTIEEIALGFVNVANETMCRPIRQLT 480
            EDQPLDIEATRGEFEKLATEINSYRK QDPSSKPMTIEEIALGF+NVANETMCRPIRQLT
Sbjct: 421  EDQPLDIEATRGEFEKLATEINSYRKIQDPSSKPMTIEEIALGFINVANETMCRPIRQLT 480

Query: 481  EMKGHETKNHALACFGGAGPQHACAIARLLGMKEIFIHRFCGILSAYGMGLADVVEEEQE 540
            EMKGHETKNHALACFGGAGPQHACAIARLLGMKEIFIHRFCGILSAYGMGLADVVEEEQE
Sbjct: 481  EMKGHETKNHALACFGGAGPQHACAIARLLGMKEIFIHRFCGILSAYGMGLADVVEEEQE 540

Query: 541  PYSAVYCSKSVQEVSRREASLLKQVKHKLRSQGFREGSINTETYLNLRYDGTDTAIMVKS 600
            PYSAVYCS+S+QEV+RREASLLKQVK KL+ QGF EGSI TETYLNLRY+GTDTAIMV+S
Sbjct: 541  PYSAVYCSESIQEVTRREASLLKQVKQKLQGQGFSEGSIKTETYLNLRYEGTDTAIMVRS 600

Query: 601  QRVDNGIEFDFAAEFEKLFQQEYGFKLQNRNILICDIRVRGVGVTNVLKPRAFEGLSGDP 660
            Q+ DNG+ FDFAA FEKLFQQEYGFKLQNRNILICDIRVRG+GVTNVLKPRAFEGL+GDP
Sbjct: 601  QKADNGVGFDFAAVFEKLFQQEYGFKLQNRNILICDIRVRGIGVTNVLKPRAFEGLAGDP 660

Query: 661  KIEGHYRVYFGNGWQDTPLFKLDNLGFGYIIPGPAIIMNGNSTVIVEPSCKATVTKYGNI 720
            KIEGHYRVYFGNGWQDTPLFKLDNLGFG++I GPAIIMNGNSTVIV+PSCKAT+TK+GNI
Sbjct: 661  KIEGHYRVYFGNGWQDTPLFKLDNLGFGHVISGPAIIMNGNSTVIVDPSCKATITKFGNI 720

Query: 721  KIEIDSTFCTKKVSEKVADVVQLSIFNHQFMGIAEQMGRTLQRTSISTNIKERLDFSCAL 780
            K+EIDS  CT+KVSEKVADVVQLSIFNH+FMGIAEQMGRTLQRTSISTNIKERLDFSCAL
Sbjct: 721  KVEIDSAVCTEKVSEKVADVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCAL 780

Query: 781  FGPDGGLVANAPHVPVHLGAMSSTVRWQIDFWGDNLNEGDVLVTNHPCAGGSHLPDITVI 840
            FGPDGGLVANAPHVPVHLGAMSSTVRWQID+WGDNLNEGDVLVTNHPCAGGSHLPDITVI
Sbjct: 781  FGPDGGLVANAPHVPVHLGAMSSTVRWQIDYWGDNLNEGDVLVTNHPCAGGSHLPDITVI 840

Query: 841  TPVFDNGKLIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVEKGIFQEEGI 900
            TPVFDNGKLIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVEKGIFQEEGI
Sbjct: 841  TPVFDNGKLIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVEKGIFQEEGI 900

Query: 901  NKLLQFPSSDEGVIPGTRRLQDNLSDLHAQVAANHRGISLIKELIVQYGLNIVQAYMTYV 960
             KLLQFP+SDEGVIPGTRRL DNLSDLHAQVAANHRGISLIKELI QYGLN VQAYMTYV
Sbjct: 901  IKLLQFPNSDEGVIPGTRRLHDNLSDLHAQVAANHRGISLIKELIAQYGLNTVQAYMTYV 960

Query: 961  QLNAEEAVREMLKSVASRVSSNSAKYVEGGSIAIEEEDYMDDGSAIHLKLTIDPHKGEAN 1020
            QLNAE AVREMLKSVASRVSSNSA   EG SI IEEEDYMDDGS IHLKLTIDP KGEAN
Sbjct: 961  QLNAEGAVREMLKSVASRVSSNSAGSAEGSSITIEEEDYMDDGSVIHLKLTIDPRKGEAN 1020

Query: 1021 FDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPPGSFLSPS 1080
            FDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPPGSFLSPS
Sbjct: 1021 FDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPPGSFLSPS 1080

Query: 1081 EKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGPSW 1140
            EKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGD TFGYYETIGGGSGAGPSW
Sbjct: 1081 EKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDSTFGYYETIGGGSGAGPSW 1140

Query: 1141 HGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGSGVYKGGDGLVREIEFKQPV 1200
            HGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGSGVYKGGDGLVREIEFKQPV
Sbjct: 1141 HGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGSGVYKGGDGLVREIEFKQPV 1200

Query: 1201 VVSILSERRVHAPRGLKGGKDGARGANFLVRKDGRRVYLGGKNTITVKAGEILQILTPGG 1260
            VVSILSERRVHAPRGL GGKDGARGANFLV+KD RRVYLGGKNT+TVKAGEILQILTPGG
Sbjct: 1201 VVSILSERRVHAPRGLNGGKDGARGANFLVKKDKRRVYLGGKNTVTVKAGEILQILTPGG 1260

Query: 1261 GGWGC 1264
            GGWGC
Sbjct: 1261 GGWGC 1265

BLAST of Cucsat.G5649 vs. ExPASy TrEMBL
Match: A0A0A0KCV4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G000060 PE=3 SV=1)

HSP 1 Score: 2531 bits (6561), Expect = 0.0
Identity = 1263/1265 (99.84%), Postives = 1264/1265 (99.92%), Query Frame = 0

Query: 1    MGSNNEEKLRFCIDRGGTFTDVYAEIPGRPDGKVFKLLSVDPSNYDDAPVEGIRRILEEY 60
            MGSNNEEKLRFCIDRGGTFTDVYAEIPGRPDGKVFKLLSVDPSNYDDAPVEGIRRILEEY
Sbjct: 53   MGSNNEEKLRFCIDRGGTFTDVYAEIPGRPDGKVFKLLSVDPSNYDDAPVEGIRRILEEY 112

Query: 61   TGKKIPRTSKIPTQNIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI 120
            TGKKIPRTSKIPTQNIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI
Sbjct: 113  TGKKIPRTSKIPTQNIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI 172

Query: 121  FDLTVSKPSNLYEDVVEVDERVELIHSKGDGNQDSSTYVEGVSGELIRIVKTLNEEALKP 180
            FDLTVSKPSNLYEDVVEVDERVELIH KGDGNQDSSTYVEGVSGELIRIVKTLNEEALKP
Sbjct: 173  FDLTVSKPSNLYEDVVEVDERVELIHGKGDGNQDSSTYVEGVSGELIRIVKTLNEEALKP 232

Query: 181  LLNDLLQRGIGCLAVVLMHSYTYPQHELALEKLALSMGFKHVSLSSALTPMVRAVPRGLT 240
            LLNDLLQRGIGCLAVVLMHSYTYPQHELALEKLALSMGFKHVSLSSALTPMVRAVPRGLT
Sbjct: 233  LLNDLLQRGIGCLAVVLMHSYTYPQHELALEKLALSMGFKHVSLSSALTPMVRAVPRGLT 292

Query: 241  ASVDAYLTPVIKEYLSGFMSKFDESSGKVNVLFMQSDGGLAPESRFSGHKAVLSGPAGGV 300
            ASVDAYLTPVIKEYLSGFMSKFDESSGKVNVLFMQSDGGLAPESRFSGHKAVLSGPAGGV
Sbjct: 293  ASVDAYLTPVIKEYLSGFMSKFDESSGKVNVLFMQSDGGLAPESRFSGHKAVLSGPAGGV 352

Query: 301  VGYSQTLFELETTKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVAA 360
            VGYSQTLFELETTKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVAA
Sbjct: 353  VGYSQTLFELETTKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVAA 412

Query: 361  GGGSKLKFQFGAFRVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPNE 420
            GGGSKLKFQFGAFRVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPNE
Sbjct: 413  GGGSKLKFQFGAFRVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPNE 472

Query: 421  DQPLDIEATRGEFEKLATEINSYRKNQDPSSKPMTIEEIALGFVNVANETMCRPIRQLTE 480
            DQPLDIEATRGEFEKLATEINSYRKNQDPSSKPMTIEEIALGFVNVANETMCRPIRQLTE
Sbjct: 473  DQPLDIEATRGEFEKLATEINSYRKNQDPSSKPMTIEEIALGFVNVANETMCRPIRQLTE 532

Query: 481  MKGHETKNHALACFGGAGPQHACAIARLLGMKEIFIHRFCGILSAYGMGLADVVEEEQEP 540
            MKGHETKNHALACFGGAGPQHACAIARLLGMKEIFIHRFCGILSAYGMGLADVVEEEQEP
Sbjct: 533  MKGHETKNHALACFGGAGPQHACAIARLLGMKEIFIHRFCGILSAYGMGLADVVEEEQEP 592

Query: 541  YSAVYCSKSVQEVSRREASLLKQVKHKLRSQGFREGSINTETYLNLRYDGTDTAIMVKSQ 600
            YSAVYCSKSVQEVSRREASLLKQVKHKLRSQGFREGSINTETYLNLRYDGTDTAIMVKSQ
Sbjct: 593  YSAVYCSKSVQEVSRREASLLKQVKHKLRSQGFREGSINTETYLNLRYDGTDTAIMVKSQ 652

Query: 601  RVDNGIEFDFAAEFEKLFQQEYGFKLQNRNILICDIRVRGVGVTNVLKPRAFEGLSGDPK 660
            RVDNG+EFDFAAEFEKLFQQEYGFKLQNRNILICDIRVRGVGVTNVLKPRAFEGLSGDPK
Sbjct: 653  RVDNGVEFDFAAEFEKLFQQEYGFKLQNRNILICDIRVRGVGVTNVLKPRAFEGLSGDPK 712

Query: 661  IEGHYRVYFGNGWQDTPLFKLDNLGFGYIIPGPAIIMNGNSTVIVEPSCKATVTKYGNIK 720
            IEGHYRVYFGNGWQDTPLFKLDNLGFGYIIPGPAIIMNGNSTVIVEPSCKATVTKYGNIK
Sbjct: 713  IEGHYRVYFGNGWQDTPLFKLDNLGFGYIIPGPAIIMNGNSTVIVEPSCKATVTKYGNIK 772

Query: 721  IEIDSTFCTKKVSEKVADVVQLSIFNHQFMGIAEQMGRTLQRTSISTNIKERLDFSCALF 780
            IEIDSTFCTKKVSEKVADVVQLSIFNHQFMGIAEQMGRTLQRTSISTNIKERLDFSCALF
Sbjct: 773  IEIDSTFCTKKVSEKVADVVQLSIFNHQFMGIAEQMGRTLQRTSISTNIKERLDFSCALF 832

Query: 781  GPDGGLVANAPHVPVHLGAMSSTVRWQIDFWGDNLNEGDVLVTNHPCAGGSHLPDITVIT 840
            GPDGGLVANAPHVPVHLGAMSSTVRWQIDFWGDNLNEGDVLVTNHPCAGGSHLPDITVIT
Sbjct: 833  GPDGGLVANAPHVPVHLGAMSSTVRWQIDFWGDNLNEGDVLVTNHPCAGGSHLPDITVIT 892

Query: 841  PVFDNGKLIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVEKGIFQEEGIN 900
            PVFDNGKLIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVEKGIFQEEGIN
Sbjct: 893  PVFDNGKLIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVEKGIFQEEGIN 952

Query: 901  KLLQFPSSDEGVIPGTRRLQDNLSDLHAQVAANHRGISLIKELIVQYGLNIVQAYMTYVQ 960
            KLLQFPSSDEGVIPGTRRLQDNLSDLHAQVAANHRGISLIKELIVQYGLNIVQAYMTYVQ
Sbjct: 953  KLLQFPSSDEGVIPGTRRLQDNLSDLHAQVAANHRGISLIKELIVQYGLNIVQAYMTYVQ 1012

Query: 961  LNAEEAVREMLKSVASRVSSNSAKYVEGGSIAIEEEDYMDDGSAIHLKLTIDPHKGEANF 1020
            LNAEEAVREMLKSVASRVSSNSAKYVEGGSIAIEEEDYMDDGSAIHLKLTIDPHKGEANF
Sbjct: 1013 LNAEEAVREMLKSVASRVSSNSAKYVEGGSIAIEEEDYMDDGSAIHLKLTIDPHKGEANF 1072

Query: 1021 DFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPPGSFLSPSE 1080
            DFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPPGSFLSPSE
Sbjct: 1073 DFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPPGSFLSPSE 1132

Query: 1081 KAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGPSWH 1140
            KAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGPSWH
Sbjct: 1133 KAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGPSWH 1192

Query: 1141 GTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGSGVYKGGDGLVREIEFKQPVV 1200
            GTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGSGVYKGGDGLVREIEFKQPVV
Sbjct: 1193 GTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGSGVYKGGDGLVREIEFKQPVV 1252

Query: 1201 VSILSERRVHAPRGLKGGKDGARGANFLVRKDGRRVYLGGKNTITVKAGEILQILTPGGG 1260
            VSILSERRVHAPRGLKGGKDGARGANFLVRKDGRRVYLGGKNTITVKAGEILQILTPGGG
Sbjct: 1253 VSILSERRVHAPRGLKGGKDGARGANFLVRKDGRRVYLGGKNTITVKAGEILQILTPGGG 1312

Query: 1261 GWGCA 1265
            GWGCA
Sbjct: 1313 GWGCA 1317

BLAST of Cucsat.G5649 vs. ExPASy TrEMBL
Match: A0A5D3BVW9 (5-oxoprolinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G00570 PE=3 SV=1)

HSP 1 Score: 2484 bits (6439), Expect = 0.0
Identity = 1238/1265 (97.87%), Postives = 1251/1265 (98.89%), Query Frame = 0

Query: 1    MGSNNEEKLRFCIDRGGTFTDVYAEIPGRPDGKVFKLLSVDPSNYDDAPVEGIRRILEEY 60
            MGSNNEEKLRFCIDRGGTFTDVYAEIPGRPDGKV KLLSVDPSNYDDAPVEGIRRILEEY
Sbjct: 1    MGSNNEEKLRFCIDRGGTFTDVYAEIPGRPDGKVLKLLSVDPSNYDDAPVEGIRRILEEY 60

Query: 61   TGKKIPRTSKIPTQNIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI 120
            TGKKIPRTSKIPTQNIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI
Sbjct: 61   TGKKIPRTSKIPTQNIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI 120

Query: 121  FDLTVSKPSNLYEDVVEVDERVELIHSKGDGNQDSSTYVEGVSGELIRIVKTLNEEALKP 180
            FDLTVSKPSNLYEDVVEVDERVELIH KGDGNQDSSTYV+GVSGELIRIVKTLNEEALKP
Sbjct: 121  FDLTVSKPSNLYEDVVEVDERVELIHGKGDGNQDSSTYVKGVSGELIRIVKTLNEEALKP 180

Query: 181  LLNDLLQRGIGCLAVVLMHSYTYPQHELALEKLALSMGFKHVSLSSALTPMVRAVPRGLT 240
            LL DLLQRGIGCLAVVLMHSYTYPQHELALEKLALSMGFKHVSLSSALTPMVRAVPRGLT
Sbjct: 181  LLKDLLQRGIGCLAVVLMHSYTYPQHELALEKLALSMGFKHVSLSSALTPMVRAVPRGLT 240

Query: 241  ASVDAYLTPVIKEYLSGFMSKFDESSGKVNVLFMQSDGGLAPESRFSGHKAVLSGPAGGV 300
            ASVDAYLTPVIKEYLSGFMSKFD+SSGKVNVLFMQSDGGLAPESRFSGHKAVLSGPAGGV
Sbjct: 241  ASVDAYLTPVIKEYLSGFMSKFDKSSGKVNVLFMQSDGGLAPESRFSGHKAVLSGPAGGV 300

Query: 301  VGYSQTLFELETTKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVAA 360
            VGYSQTLFELETTKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVAA
Sbjct: 301  VGYSQTLFELETTKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVAA 360

Query: 361  GGGSKLKFQFGAFRVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPNE 420
            GGGSKLKFQFGAFRVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPNE
Sbjct: 361  GGGSKLKFQFGAFRVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPNE 420

Query: 421  DQPLDIEATRGEFEKLATEINSYRKNQDPSSKPMTIEEIALGFVNVANETMCRPIRQLTE 480
            DQPLDIEATRGEFEKLATEINSYRKNQDPSSKPMTIE+IALGFVNVANETMCRPIRQLTE
Sbjct: 421  DQPLDIEATRGEFEKLATEINSYRKNQDPSSKPMTIEQIALGFVNVANETMCRPIRQLTE 480

Query: 481  MKGHETKNHALACFGGAGPQHACAIARLLGMKEIFIHRFCGILSAYGMGLADVVEEEQEP 540
            MKGHETKNHALACFGGAGPQHACAIARLLGMKEIFIHRFCGILSAYGMGLADVVEEEQEP
Sbjct: 481  MKGHETKNHALACFGGAGPQHACAIARLLGMKEIFIHRFCGILSAYGMGLADVVEEEQEP 540

Query: 541  YSAVYCSKSVQEVSRREASLLKQVKHKLRSQGFREGSINTETYLNLRYDGTDTAIMVKSQ 600
            YSAVYCS+S  EVSRREASLLKQVKHKL+SQGFREGSI TETYLNLRYDGTDTAIMVK Q
Sbjct: 541  YSAVYCSESFLEVSRREASLLKQVKHKLQSQGFREGSIKTETYLNLRYDGTDTAIMVKGQ 600

Query: 601  RVDNGIEFDFAAEFEKLFQQEYGFKLQNRNILICDIRVRGVGVTNVLKPRAFEGLSGDPK 660
            R DNG+EFDFAAEFEKLFQQEYGFKLQNRNILICDIRVRGVGVTNVLKPRAFEGLSGDPK
Sbjct: 601  RADNGVEFDFAAEFEKLFQQEYGFKLQNRNILICDIRVRGVGVTNVLKPRAFEGLSGDPK 660

Query: 661  IEGHYRVYFGNGWQDTPLFKLDNLGFGYIIPGPAIIMNGNSTVIVEPSCKATVTKYGNIK 720
            IEGHYRVYFGNGWQDTPL KLDNLGFG+IIPGPAIIMNGNSTVIVEPSCKATVTKYGNIK
Sbjct: 661  IEGHYRVYFGNGWQDTPLLKLDNLGFGHIIPGPAIIMNGNSTVIVEPSCKATVTKYGNIK 720

Query: 721  IEIDSTFCTKKVSEKVADVVQLSIFNHQFMGIAEQMGRTLQRTSISTNIKERLDFSCALF 780
            IEIDSTFCT+ VSEKVADVVQLSIFNH+FMGIAEQMGRTLQRTSISTNIKERLDFSCALF
Sbjct: 721  IEIDSTFCTENVSEKVADVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCALF 780

Query: 781  GPDGGLVANAPHVPVHLGAMSSTVRWQIDFWGDNLNEGDVLVTNHPCAGGSHLPDITVIT 840
            GPDGGLVANAPHVPVHLGAMSSTVRWQID+WGDNLNEGDVLVTNHPCAGGSHLPDITVIT
Sbjct: 781  GPDGGLVANAPHVPVHLGAMSSTVRWQIDYWGDNLNEGDVLVTNHPCAGGSHLPDITVIT 840

Query: 841  PVFDNGKLIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVEKGIFQEEGIN 900
            PVFDNGKLIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVEKGIFQEEGI 
Sbjct: 841  PVFDNGKLIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVEKGIFQEEGII 900

Query: 901  KLLQFPSSDEGVIPGTRRLQDNLSDLHAQVAANHRGISLIKELIVQYGLNIVQAYMTYVQ 960
            KLLQFPSSDEGVIPGTRRLQDNLSDLHAQVAANHRGISLIKELIVQYGLNIVQAYMTYVQ
Sbjct: 901  KLLQFPSSDEGVIPGTRRLQDNLSDLHAQVAANHRGISLIKELIVQYGLNIVQAYMTYVQ 960

Query: 961  LNAEEAVREMLKSVASRVSSNSAKYVEGGSIAIEEEDYMDDGSAIHLKLTIDPHKGEANF 1020
            LNAEEAVREMLKSVASRVSSNSA+ VEGGSI IEEEDYMDDGSAIHLKLTIDP+KGEANF
Sbjct: 961  LNAEEAVREMLKSVASRVSSNSARSVEGGSIVIEEEDYMDDGSAIHLKLTIDPNKGEANF 1020

Query: 1021 DFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPPGSFLSPSE 1080
            DFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPPGSFLSPSE
Sbjct: 1021 DFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPPGSFLSPSE 1080

Query: 1081 KAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGPSWH 1140
            KAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGPSWH
Sbjct: 1081 KAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGPSWH 1140

Query: 1141 GTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGSGVYKGGDGLVREIEFKQPVV 1200
            GTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGSGVYKGGDGLVREIEFKQPVV
Sbjct: 1141 GTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGSGVYKGGDGLVREIEFKQPVV 1200

Query: 1201 VSILSERRVHAPRGLKGGKDGARGANFLVRKDGRRVYLGGKNTITVKAGEILQILTPGGG 1260
            VSILSERRVHAPRGLKGGKDGA GANFLVRKDGRRVYLGGKNT+TVKAGEILQILTPGGG
Sbjct: 1201 VSILSERRVHAPRGLKGGKDGAHGANFLVRKDGRRVYLGGKNTVTVKAGEILQILTPGGG 1260

Query: 1261 GWGCA 1265
            GWGCA
Sbjct: 1261 GWGCA 1265

BLAST of Cucsat.G5649 vs. ExPASy TrEMBL
Match: A0A1S3C7K4 (5-oxoprolinase OS=Cucumis melo OX=3656 GN=LOC103497767 PE=3 SV=1)

HSP 1 Score: 2484 bits (6439), Expect = 0.0
Identity = 1238/1265 (97.87%), Postives = 1251/1265 (98.89%), Query Frame = 0

Query: 1    MGSNNEEKLRFCIDRGGTFTDVYAEIPGRPDGKVFKLLSVDPSNYDDAPVEGIRRILEEY 60
            MGSNNEEKLRFCIDRGGTFTDVYAEIPGRPDGKV KLLSVDPSNYDDAPVEGIRRILEEY
Sbjct: 1    MGSNNEEKLRFCIDRGGTFTDVYAEIPGRPDGKVLKLLSVDPSNYDDAPVEGIRRILEEY 60

Query: 61   TGKKIPRTSKIPTQNIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI 120
            TGKKIPRTSKIPTQNIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI
Sbjct: 61   TGKKIPRTSKIPTQNIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI 120

Query: 121  FDLTVSKPSNLYEDVVEVDERVELIHSKGDGNQDSSTYVEGVSGELIRIVKTLNEEALKP 180
            FDLTVSKPSNLYEDVVEVDERVELIH KGDGNQDSSTYV+GVSGELIRIVKTLNEEALKP
Sbjct: 121  FDLTVSKPSNLYEDVVEVDERVELIHGKGDGNQDSSTYVKGVSGELIRIVKTLNEEALKP 180

Query: 181  LLNDLLQRGIGCLAVVLMHSYTYPQHELALEKLALSMGFKHVSLSSALTPMVRAVPRGLT 240
            LL DLLQRGIGCLAVVLMHSYTYPQHELALEKLALSMGFKHVSLSSALTPMVRAVPRGLT
Sbjct: 181  LLKDLLQRGIGCLAVVLMHSYTYPQHELALEKLALSMGFKHVSLSSALTPMVRAVPRGLT 240

Query: 241  ASVDAYLTPVIKEYLSGFMSKFDESSGKVNVLFMQSDGGLAPESRFSGHKAVLSGPAGGV 300
            ASVDAYLTPVIKEYLSGFMSKFD+SSGKVNVLFMQSDGGLAPESRFSGHKAVLSGPAGGV
Sbjct: 241  ASVDAYLTPVIKEYLSGFMSKFDKSSGKVNVLFMQSDGGLAPESRFSGHKAVLSGPAGGV 300

Query: 301  VGYSQTLFELETTKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVAA 360
            VGYSQTLFELETTKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVAA
Sbjct: 301  VGYSQTLFELETTKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVAA 360

Query: 361  GGGSKLKFQFGAFRVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPNE 420
            GGGSKLKFQFGAFRVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPNE
Sbjct: 361  GGGSKLKFQFGAFRVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPNE 420

Query: 421  DQPLDIEATRGEFEKLATEINSYRKNQDPSSKPMTIEEIALGFVNVANETMCRPIRQLTE 480
            DQPLDIEATRGEFEKLATEINSYRKNQDPSSKPMTIE+IALGFVNVANETMCRPIRQLTE
Sbjct: 421  DQPLDIEATRGEFEKLATEINSYRKNQDPSSKPMTIEQIALGFVNVANETMCRPIRQLTE 480

Query: 481  MKGHETKNHALACFGGAGPQHACAIARLLGMKEIFIHRFCGILSAYGMGLADVVEEEQEP 540
            MKGHETKNHALACFGGAGPQHACAIARLLGMKEIFIHRFCGILSAYGMGLADVVEEEQEP
Sbjct: 481  MKGHETKNHALACFGGAGPQHACAIARLLGMKEIFIHRFCGILSAYGMGLADVVEEEQEP 540

Query: 541  YSAVYCSKSVQEVSRREASLLKQVKHKLRSQGFREGSINTETYLNLRYDGTDTAIMVKSQ 600
            YSAVYCS+S  EVSRREASLLKQVKHKL+SQGFREGSI TETYLNLRYDGTDTAIMVK Q
Sbjct: 541  YSAVYCSESFLEVSRREASLLKQVKHKLQSQGFREGSIKTETYLNLRYDGTDTAIMVKGQ 600

Query: 601  RVDNGIEFDFAAEFEKLFQQEYGFKLQNRNILICDIRVRGVGVTNVLKPRAFEGLSGDPK 660
            R DNG+EFDFAAEFEKLFQQEYGFKLQNRNILICDIRVRGVGVTNVLKPRAFEGLSGDPK
Sbjct: 601  RADNGVEFDFAAEFEKLFQQEYGFKLQNRNILICDIRVRGVGVTNVLKPRAFEGLSGDPK 660

Query: 661  IEGHYRVYFGNGWQDTPLFKLDNLGFGYIIPGPAIIMNGNSTVIVEPSCKATVTKYGNIK 720
            IEGHYRVYFGNGWQDTPL KLDNLGFG+IIPGPAIIMNGNSTVIVEPSCKATVTKYGNIK
Sbjct: 661  IEGHYRVYFGNGWQDTPLLKLDNLGFGHIIPGPAIIMNGNSTVIVEPSCKATVTKYGNIK 720

Query: 721  IEIDSTFCTKKVSEKVADVVQLSIFNHQFMGIAEQMGRTLQRTSISTNIKERLDFSCALF 780
            IEIDSTFCT+ VSEKVADVVQLSIFNH+FMGIAEQMGRTLQRTSISTNIKERLDFSCALF
Sbjct: 721  IEIDSTFCTENVSEKVADVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCALF 780

Query: 781  GPDGGLVANAPHVPVHLGAMSSTVRWQIDFWGDNLNEGDVLVTNHPCAGGSHLPDITVIT 840
            GPDGGLVANAPHVPVHLGAMSSTVRWQID+WGDNLNEGDVLVTNHPCAGGSHLPDITVIT
Sbjct: 781  GPDGGLVANAPHVPVHLGAMSSTVRWQIDYWGDNLNEGDVLVTNHPCAGGSHLPDITVIT 840

Query: 841  PVFDNGKLIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVEKGIFQEEGIN 900
            PVFDNGKLIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVEKGIFQEEGI 
Sbjct: 841  PVFDNGKLIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVEKGIFQEEGII 900

Query: 901  KLLQFPSSDEGVIPGTRRLQDNLSDLHAQVAANHRGISLIKELIVQYGLNIVQAYMTYVQ 960
            KLLQFPSSDEGVIPGTRRLQDNLSDLHAQVAANHRGISLIKELIVQYGLNIVQAYMTYVQ
Sbjct: 901  KLLQFPSSDEGVIPGTRRLQDNLSDLHAQVAANHRGISLIKELIVQYGLNIVQAYMTYVQ 960

Query: 961  LNAEEAVREMLKSVASRVSSNSAKYVEGGSIAIEEEDYMDDGSAIHLKLTIDPHKGEANF 1020
            LNAEEAVREMLKSVASRVSSNSA+ VEGGSI IEEEDYMDDGSAIHLKLTIDP+KGEANF
Sbjct: 961  LNAEEAVREMLKSVASRVSSNSARSVEGGSIVIEEEDYMDDGSAIHLKLTIDPNKGEANF 1020

Query: 1021 DFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPPGSFLSPSE 1080
            DFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPPGSFLSPSE
Sbjct: 1021 DFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPPGSFLSPSE 1080

Query: 1081 KAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGPSWH 1140
            KAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGPSWH
Sbjct: 1081 KAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGPSWH 1140

Query: 1141 GTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGSGVYKGGDGLVREIEFKQPVV 1200
            GTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGSGVYKGGDGLVREIEFKQPVV
Sbjct: 1141 GTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGSGVYKGGDGLVREIEFKQPVV 1200

Query: 1201 VSILSERRVHAPRGLKGGKDGARGANFLVRKDGRRVYLGGKNTITVKAGEILQILTPGGG 1260
            VSILSERRVHAPRGLKGGKDGA GANFLVRKDGRRVYLGGKNT+TVKAGEILQILTPGGG
Sbjct: 1201 VSILSERRVHAPRGLKGGKDGAHGANFLVRKDGRRVYLGGKNTVTVKAGEILQILTPGGG 1260

Query: 1261 GWGCA 1265
            GWGCA
Sbjct: 1261 GWGCA 1265

BLAST of Cucsat.G5649 vs. ExPASy TrEMBL
Match: A0A6J1E9D6 (5-oxoprolinase OS=Cucurbita moschata OX=3662 GN=LOC111431917 PE=3 SV=1)

HSP 1 Score: 2410 bits (6246), Expect = 0.0
Identity = 1197/1265 (94.62%), Postives = 1229/1265 (97.15%), Query Frame = 0

Query: 1    MGSNNEEKLRFCIDRGGTFTDVYAEIPGRPDGKVFKLLSVDPSNYDDAPVEGIRRILEEY 60
            MGS+NE+KLRFCIDRGGTFTDVYAEIPGRPDGKV KLLSVDPSNYDDAPVEGIRRILEEY
Sbjct: 1    MGSSNEDKLRFCIDRGGTFTDVYAEIPGRPDGKVIKLLSVDPSNYDDAPVEGIRRILEEY 60

Query: 61   TGKKIPRTSKIPTQNIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI 120
             GKKIPRTSKIPTQNIEWIRMGTTVATNALLER+GERIALCVTKGFRDLLQIGNQARP+I
Sbjct: 61   GGKKIPRTSKIPTQNIEWIRMGTTVATNALLERRGERIALCVTKGFRDLLQIGNQARPNI 120

Query: 121  FDLTVSKPSNLYEDVVEVDERVELIHSKGDGNQDSST-YVEGVSGELIRIVKTLNEEALK 180
            FDLTVSKPSNLYEDV+EVDERVEL+H KGD NQDSST YV+GVSGELI IVKTLNEEALK
Sbjct: 121  FDLTVSKPSNLYEDVIEVDERVELVHGKGDDNQDSSTSYVKGVSGELIHIVKTLNEEALK 180

Query: 181  PLLNDLLQRGIGCLAVVLMHSYTYPQHELALEKLALSMGFKHVSLSSALTPMVRAVPRGL 240
            PLL DLLQRGI CLAVVLMHSYTYPQHELALEKLA SMGFKHVSLSSALTPMVRAVPRGL
Sbjct: 181  PLLKDLLQRGISCLAVVLMHSYTYPQHELALEKLARSMGFKHVSLSSALTPMVRAVPRGL 240

Query: 241  TASVDAYLTPVIKEYLSGFMSKFDESSGKVNVLFMQSDGGLAPESRFSGHKAVLSGPAGG 300
            TASVDAYLTPVIKEYLSGFMSKFDESSGKVNVLFMQSDGGLAPE+RFSGHKAVLSGPAGG
Sbjct: 241  TASVDAYLTPVIKEYLSGFMSKFDESSGKVNVLFMQSDGGLAPENRFSGHKAVLSGPAGG 300

Query: 301  VVGYSQTLFELETTKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVA 360
            VVGYSQTLF+LET KPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVA
Sbjct: 301  VVGYSQTLFDLETRKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVA 360

Query: 361  AGGGSKLKFQFGAFRVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPN 420
            AGGGSKLKFQFGAFRVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPN
Sbjct: 361  AGGGSKLKFQFGAFRVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPN 420

Query: 421  EDQPLDIEATRGEFEKLATEINSYRKNQDPSSKPMTIEEIALGFVNVANETMCRPIRQLT 480
            EDQPLDIEATRGEFEKLATEINSYRK QDPSSKPMTIEEIALGF+NVANETMCRPIRQLT
Sbjct: 421  EDQPLDIEATRGEFEKLATEINSYRKIQDPSSKPMTIEEIALGFINVANETMCRPIRQLT 480

Query: 481  EMKGHETKNHALACFGGAGPQHACAIARLLGMKEIFIHRFCGILSAYGMGLADVVEEEQE 540
            EMKGHETKNHALACFGGAGPQHACAIARLLGMKEIFIHRFCGILSAYGMGLADVVEEEQE
Sbjct: 481  EMKGHETKNHALACFGGAGPQHACAIARLLGMKEIFIHRFCGILSAYGMGLADVVEEEQE 540

Query: 541  PYSAVYCSKSVQEVSRREASLLKQVKHKLRSQGFREGSINTETYLNLRYDGTDTAIMVKS 600
            PYSAVYCS+S+QEV+RREASLLKQVK KL+ QGF EGSI TETYLNLRY+GTDTAIMV+S
Sbjct: 541  PYSAVYCSESIQEVTRREASLLKQVKQKLQGQGFSEGSIKTETYLNLRYEGTDTAIMVRS 600

Query: 601  QRVDNGIEFDFAAEFEKLFQQEYGFKLQNRNILICDIRVRGVGVTNVLKPRAFEGLSGDP 660
            Q+ DNG+ FDFAA FEKLFQQEYGFKLQNRNILICDIRVRG+GVTNVLKPRAFEGL+GDP
Sbjct: 601  QKADNGVGFDFAAVFEKLFQQEYGFKLQNRNILICDIRVRGIGVTNVLKPRAFEGLAGDP 660

Query: 661  KIEGHYRVYFGNGWQDTPLFKLDNLGFGYIIPGPAIIMNGNSTVIVEPSCKATVTKYGNI 720
            KIEGHYRVYFGNGWQDTPLFKLDNLGFG++I GPAIIMNGNSTVIV+PSCKAT+TK+GNI
Sbjct: 661  KIEGHYRVYFGNGWQDTPLFKLDNLGFGHVISGPAIIMNGNSTVIVDPSCKATITKFGNI 720

Query: 721  KIEIDSTFCTKKVSEKVADVVQLSIFNHQFMGIAEQMGRTLQRTSISTNIKERLDFSCAL 780
            K+EIDS  CT+KVSEKVADVVQLSIFNH+FMGIAEQMGRTLQRTSISTNIKERLDFSCAL
Sbjct: 721  KVEIDSAVCTEKVSEKVADVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCAL 780

Query: 781  FGPDGGLVANAPHVPVHLGAMSSTVRWQIDFWGDNLNEGDVLVTNHPCAGGSHLPDITVI 840
            FGPDGGLVANAPHVPVHLGAMSSTVRWQID+WGDNLNEGDVLVTNHPCAGGSHLPDITVI
Sbjct: 781  FGPDGGLVANAPHVPVHLGAMSSTVRWQIDYWGDNLNEGDVLVTNHPCAGGSHLPDITVI 840

Query: 841  TPVFDNGKLIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVEKGIFQEEGI 900
            TPVFDNGKLIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVEKGIFQEEGI
Sbjct: 841  TPVFDNGKLIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVEKGIFQEEGI 900

Query: 901  NKLLQFPSSDEGVIPGTRRLQDNLSDLHAQVAANHRGISLIKELIVQYGLNIVQAYMTYV 960
             KLLQFP+SDEGVIPGTRRL DNLSDLHAQVAANHRGISLIKELI QYGLN VQAYMTYV
Sbjct: 901  IKLLQFPNSDEGVIPGTRRLHDNLSDLHAQVAANHRGISLIKELIAQYGLNTVQAYMTYV 960

Query: 961  QLNAEEAVREMLKSVASRVSSNSAKYVEGGSIAIEEEDYMDDGSAIHLKLTIDPHKGEAN 1020
            QLNAE AVREMLKSVASRVSSNSA   EG SI IEEEDYMDDGS IHLKLTIDP KGEAN
Sbjct: 961  QLNAEGAVREMLKSVASRVSSNSAGSAEGSSITIEEEDYMDDGSVIHLKLTIDPRKGEAN 1020

Query: 1021 FDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPPGSFLSPS 1080
            FDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPPGSFLSPS
Sbjct: 1021 FDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPPGSFLSPS 1080

Query: 1081 EKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGPSW 1140
            EKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGD TFGYYETIGGGSGAGPSW
Sbjct: 1081 EKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDSTFGYYETIGGGSGAGPSW 1140

Query: 1141 HGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGSGVYKGGDGLVREIEFKQPV 1200
            HGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGSGVYKGGDGLVREIEFKQPV
Sbjct: 1141 HGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGSGVYKGGDGLVREIEFKQPV 1200

Query: 1201 VVSILSERRVHAPRGLKGGKDGARGANFLVRKDGRRVYLGGKNTITVKAGEILQILTPGG 1260
            VVSILSERRVHAPRGL GGKDGARGANFLV+KD RRVYLGGKNT+TVKAGEILQILTPGG
Sbjct: 1201 VVSILSERRVHAPRGLNGGKDGARGANFLVKKDKRRVYLGGKNTVTVKAGEILQILTPGG 1260

Query: 1261 GGWGC 1264
            GGWGC
Sbjct: 1261 GGWGC 1265

BLAST of Cucsat.G5649 vs. ExPASy TrEMBL
Match: A0A6J1ISY5 (5-oxoprolinase OS=Cucurbita maxima OX=3661 GN=LOC111479649 PE=3 SV=1)

HSP 1 Score: 2404 bits (6231), Expect = 0.0
Identity = 1196/1265 (94.55%), Postives = 1228/1265 (97.08%), Query Frame = 0

Query: 1    MGSNNEEKLRFCIDRGGTFTDVYAEIPGRPDGKVFKLLSVDPSNYDDAPVEGIRRILEEY 60
            MGS+NE+KLRFCIDRGGTFTDVYAEIPG PDGKV KLLSVDPSNYDDAPVEGIRRILEEY
Sbjct: 1    MGSSNEDKLRFCIDRGGTFTDVYAEIPGLPDGKVIKLLSVDPSNYDDAPVEGIRRILEEY 60

Query: 61   TGKKIPRTSKIPTQNIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI 120
             GKKIPRTSKIPTQNIEWIRMGTTVATNALLER+GERIALCVTKGFRDLLQIGNQARP+I
Sbjct: 61   GGKKIPRTSKIPTQNIEWIRMGTTVATNALLERRGERIALCVTKGFRDLLQIGNQARPNI 120

Query: 121  FDLTVSKPSNLYEDVVEVDERVELIHSKGDGNQDSST-YVEGVSGELIRIVKTLNEEALK 180
            FDLTVSKPSNLYEDV+EVDERVEL+H KGD NQDSST YV+GVSGELI IVKTLNEEALK
Sbjct: 121  FDLTVSKPSNLYEDVIEVDERVELVHGKGDDNQDSSTSYVKGVSGELIHIVKTLNEEALK 180

Query: 181  PLLNDLLQRGIGCLAVVLMHSYTYPQHELALEKLALSMGFKHVSLSSALTPMVRAVPRGL 240
            PLL DLLQRGI CLAVVLMHSYTYPQHELALEKLA SMGFKHVSLSSALTPMVRAVPRGL
Sbjct: 181  PLLKDLLQRGISCLAVVLMHSYTYPQHELALEKLARSMGFKHVSLSSALTPMVRAVPRGL 240

Query: 241  TASVDAYLTPVIKEYLSGFMSKFDESSGKVNVLFMQSDGGLAPESRFSGHKAVLSGPAGG 300
            TASVDAYLTPVIKEYLSGFMSKFDESSGKVNVLFMQSDGGLAPE+RFSGHKAVLSGPAGG
Sbjct: 241  TASVDAYLTPVIKEYLSGFMSKFDESSGKVNVLFMQSDGGLAPENRFSGHKAVLSGPAGG 300

Query: 301  VVGYSQTLFELETTKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVA 360
            VVGYSQTLF+LET KPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVA
Sbjct: 301  VVGYSQTLFDLETRKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVA 360

Query: 361  AGGGSKLKFQFGAFRVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPN 420
            AGGGSKLKFQFGAFRVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPN
Sbjct: 361  AGGGSKLKFQFGAFRVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPN 420

Query: 421  EDQPLDIEATRGEFEKLATEINSYRKNQDPSSKPMTIEEIALGFVNVANETMCRPIRQLT 480
            EDQPLDIEATRGEFEKLATEINSYRK QDPSSKPMTIEEIALGFVNVANETMCRPIRQLT
Sbjct: 421  EDQPLDIEATRGEFEKLATEINSYRKIQDPSSKPMTIEEIALGFVNVANETMCRPIRQLT 480

Query: 481  EMKGHETKNHALACFGGAGPQHACAIARLLGMKEIFIHRFCGILSAYGMGLADVVEEEQE 540
            EMKGHETKNHALACFGGAGPQHACAIARLLGMKEIFIHRFCGILSAYGMGLADVVEEEQE
Sbjct: 481  EMKGHETKNHALACFGGAGPQHACAIARLLGMKEIFIHRFCGILSAYGMGLADVVEEEQE 540

Query: 541  PYSAVYCSKSVQEVSRREASLLKQVKHKLRSQGFREGSINTETYLNLRYDGTDTAIMVKS 600
            PYSAVYCS+S+QEV+RREASLLKQVK KL+ QGF EGSI TETYLNLRY+GTDTAIMV+S
Sbjct: 541  PYSAVYCSESIQEVTRREASLLKQVKQKLQGQGFSEGSIKTETYLNLRYEGTDTAIMVRS 600

Query: 601  QRVDNGIEFDFAAEFEKLFQQEYGFKLQNRNILICDIRVRGVGVTNVLKPRAFEGLSGDP 660
            Q+VD+G+ FDFAA FEKLFQQEYGFKLQN+NILICDIRVRG+GVTNVLKPRAFEGL+GDP
Sbjct: 601  QKVDDGVGFDFAAVFEKLFQQEYGFKLQNKNILICDIRVRGIGVTNVLKPRAFEGLAGDP 660

Query: 661  KIEGHYRVYFGNGWQDTPLFKLDNLGFGYIIPGPAIIMNGNSTVIVEPSCKATVTKYGNI 720
            KIEGHYRVYFGNGWQDTPLFKLDNLGFG++I GPAIIMNGNSTVIVEPSCKAT+TK+GNI
Sbjct: 661  KIEGHYRVYFGNGWQDTPLFKLDNLGFGHVISGPAIIMNGNSTVIVEPSCKATITKFGNI 720

Query: 721  KIEIDSTFCTKKVSEKVADVVQLSIFNHQFMGIAEQMGRTLQRTSISTNIKERLDFSCAL 780
            KIEIDS FCT+KVSEKVADVVQLSIFNH+FMGIAEQMGRTLQRTSISTNIKERLDFSCAL
Sbjct: 721  KIEIDSAFCTEKVSEKVADVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCAL 780

Query: 781  FGPDGGLVANAPHVPVHLGAMSSTVRWQIDFWGDNLNEGDVLVTNHPCAGGSHLPDITVI 840
            FGPDGGLVANAPHVPVHLGAMSSTVRWQI++WGDNLNEGDVLVTNHPCAGGSHLPDITVI
Sbjct: 781  FGPDGGLVANAPHVPVHLGAMSSTVRWQIEYWGDNLNEGDVLVTNHPCAGGSHLPDITVI 840

Query: 841  TPVFDNGKLIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVEKGIFQEEGI 900
            TPVFDNGKLIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVEKGIFQEEGI
Sbjct: 841  TPVFDNGKLIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVEKGIFQEEGI 900

Query: 901  NKLLQFPSSDEGVIPGTRRLQDNLSDLHAQVAANHRGISLIKELIVQYGLNIVQAYMTYV 960
             KLLQFP+SDEGVIPGTRRL DNLSDLHAQVAANHRGISLIKELI QYGLN VQAYMTYV
Sbjct: 901  IKLLQFPNSDEGVIPGTRRLHDNLSDLHAQVAANHRGISLIKELIAQYGLNTVQAYMTYV 960

Query: 961  QLNAEEAVREMLKSVASRVSSNSAKYVEGGSIAIEEEDYMDDGSAIHLKLTIDPHKGEAN 1020
            QLNAE AVREMLKSVASRVSSNS    EG SI IEEEDYMDDGS IHLKLTIDP KGEAN
Sbjct: 961  QLNAEGAVREMLKSVASRVSSNSTGSAEGSSITIEEEDYMDDGSVIHLKLTIDPRKGEAN 1020

Query: 1021 FDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPPGSFLSPS 1080
            FDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPPGSFLSPS
Sbjct: 1021 FDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPPGSFLSPS 1080

Query: 1081 EKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGPSW 1140
            EKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGD TFGYYETIGGGSGAGPSW
Sbjct: 1081 EKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDSTFGYYETIGGGSGAGPSW 1140

Query: 1141 HGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGSGVYKGGDGLVREIEFKQPV 1200
            HGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGSGVYKGGDGLVREIEFKQPV
Sbjct: 1141 HGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGSGVYKGGDGLVREIEFKQPV 1200

Query: 1201 VVSILSERRVHAPRGLKGGKDGARGANFLVRKDGRRVYLGGKNTITVKAGEILQILTPGG 1260
            VVSILSERRVHAPRGL GGKDGA GANFLV+KD RRVYLGGKNT+TVKAGEILQILTPGG
Sbjct: 1201 VVSILSERRVHAPRGLNGGKDGACGANFLVKKDKRRVYLGGKNTVTVKAGEILQILTPGG 1260

Query: 1261 GGWGC 1264
            GGWGC
Sbjct: 1261 GGWGC 1265

BLAST of Cucsat.G5649 vs. TAIR 10
Match: AT5G37830.1 (oxoprolinase 1 )

HSP 1 Score: 2140.2 bits (5544), Expect = 0.0e+00
Identity = 1044/1265 (82.53%), Postives = 1149/1265 (90.83%), Query Frame = 0

Query: 1    MGSNNEEKLRFCIDRGGTFTDVYAEIPGRPDGKVFKLLSVDPSNYDDAPVEGIRRILEEY 60
            MG+  E KLRFCIDRGGTFTDVYAEIPG  DG V KLLSVDPSNYDDAPVEGIRRILEEY
Sbjct: 1    MGTVIEGKLRFCIDRGGTFTDVYAEIPGHSDGHVLKLLSVDPSNYDDAPVEGIRRILEEY 60

Query: 61   TGKKIPRTSKIPTQNIEWIRMGTTVATNALLERKGERIALCVTKGFRDLLQIGNQARPDI 120
            TGKKIPRTSKIPT  I+WIRMGTTVATNALLERKGERIALCVTKGF+DLLQIGNQARPDI
Sbjct: 61   TGKKIPRTSKIPTDKIQWIRMGTTVATNALLERKGERIALCVTKGFKDLLQIGNQARPDI 120

Query: 121  FDLTVSKPSNLYEDVVEVDERVELIHSKGDGNQDSSTYVEGVSGELIRIVKTLNEEALKP 180
            FDLTV+KPSNLYE+V+EVDERV L  +  D + D  + ++GVSGE +R+VK  + E LKP
Sbjct: 121  FDLTVAKPSNLYEEVIEVDERVVL--ALEDDDDDEGSLIKGVSGEFLRVVKPFDGEGLKP 180

Query: 181  LLNDLLQRGIGCLAVVLMHSYTYPQHELALEKLALSMGFKHVSLSSALTPMVRAVPRGLT 240
            LL  LL +GI CLAVVLMHSYTYP+HE+ +EKLAL MGF+HVSLSSALTPMVRAVPRGLT
Sbjct: 181  LLKGLLDKGISCLAVVLMHSYTYPKHEMDVEKLALEMGFRHVSLSSALTPMVRAVPRGLT 240

Query: 241  ASVDAYLTPVIKEYLSGFMSKFDESSGKVNVLFMQSDGGLAPESRFSGHKAVLSGPAGGV 300
            A+VDAYLTPVIKEYLSGF+SKFD+  GKVNVLFMQSDGGLAPESRFSGHKAVLSGPAGGV
Sbjct: 241  ATVDAYLTPVIKEYLSGFISKFDDDLGKVNVLFMQSDGGLAPESRFSGHKAVLSGPAGGV 300

Query: 301  VGYSQTLFELETTKPLIGFDMGGTSTDVSRYAGSYEQVLETQIAGAIIQAPQLDINTVAA 360
            VGYSQTLF LET KPLIGFDMGGTSTDVSRY GSYEQV+ETQIAG IIQAPQLDINTVAA
Sbjct: 301  VGYSQTLFGLETEKPLIGFDMGGTSTDVSRYDGSYEQVIETQIAGTIIQAPQLDINTVAA 360

Query: 361  GGGSKLKFQFGAFRVGPESVGAHPGPVCYRKGGELAVTDANLVLGFVIPDFFPSIFGPNE 420
            GGGSKLKFQFGAFRVGP+SVGAHPGPVCYRKGGELAVTDANLVLGFVIPD+FPSIFGPNE
Sbjct: 361  GGGSKLKFQFGAFRVGPDSVGAHPGPVCYRKGGELAVTDANLVLGFVIPDYFPSIFGPNE 420

Query: 421  DQPLDIEATRGEFEKLATEINSYRKNQDPSSKPMTIEEIALGFVNVANETMCRPIRQLTE 480
            DQPLD+ ATR  FEKLA +IN YRK+QDPS+K M++EEIA+GFV+VANETMCRPIRQLTE
Sbjct: 421  DQPLDVAATREAFEKLAGQINIYRKSQDPSAKDMSVEEIAMGFVSVANETMCRPIRQLTE 480

Query: 481  MKGHETKNHALACFGGAGPQHACAIARLLGMKEIFIHRFCGILSAYGMGLADVVEEEQEP 540
            MKGHETKNHALACFGGAGPQHACAIAR LGMKE+ +HR+CGILSAYGMGLADV+E+ QEP
Sbjct: 481  MKGHETKNHALACFGGAGPQHACAIARSLGMKEVLVHRYCGILSAYGMGLADVIEDAQEP 540

Query: 541  YSAVYCSKSVQEVSRREASLLKQVKHKLRSQGFREGSINTETYLNLRYDGTDTAIMVKSQ 600
            YSAVY  +S+ EV RRE  LL++V+ KL+ QGF +G+I+TETYLNLRYDGTDTAIMVK +
Sbjct: 541  YSAVYGPESLSEVFRRETVLLREVREKLQEQGFGDGNISTETYLNLRYDGTDTAIMVKGK 600

Query: 601  RVDNGIEFDFAAEFEKLFQQEYGFKLQNRNILICDIRVRGVGVTNVLKPRAFEGLSGDPK 660
            +  +G  FD+AAEF KLF+QEYGFKLQNRN+LICD+RVRG+GVT++LKPRA E     PK
Sbjct: 601  KTGDGSAFDYAAEFLKLFEQEYGFKLQNRNLLICDVRVRGIGVTSILKPRAVEAAPVTPK 660

Query: 661  IEGHYRVYFGNGWQDTPLFKLDNLGFGYIIPGPAIIMNGNSTVIVEPSCKATVTKYGNIK 720
            +E HY+VYF  GW DTPLFKL+NLGFG+ I GPAIIMNGNSTVIVEP CKA +TKYGNIK
Sbjct: 661  VERHYKVYFEGGWHDTPLFKLENLGFGHEILGPAIIMNGNSTVIVEPQCKAIITKYGNIK 720

Query: 721  IEIDSTFCTKKVSEKVADVVQLSIFNHQFMGIAEQMGRTLQRTSISTNIKERLDFSCALF 780
            IE++    + K++E VADVVQLSIFNH+FMGIAEQMGRTLQRTSISTNIKERLDFSCALF
Sbjct: 721  IEVEPATSSVKLAENVADVVQLSIFNHRFMGIAEQMGRTLQRTSISTNIKERLDFSCALF 780

Query: 781  GPDGGLVANAPHVPVHLGAMSSTVRWQIDFWGDNLNEGDVLVTNHPCAGGSHLPDITVIT 840
             PDGGLVANAPHVPVHLGAMSSTVRWQ+  WG+NLNEGDVLVTNHPCAGGSHLPDITVIT
Sbjct: 781  SPDGGLVANAPHVPVHLGAMSSTVRWQLKHWGENLNEGDVLVTNHPCAGGSHLPDITVIT 840

Query: 841  PVFDNGKLIFFVASRGHHAEIGGITPGSMPPFSKSIWEEGAAIKAFKLVEKGIFQEEGIN 900
            PVFD GKL+FFVASRGHHAE+GGITPGSMPPFSK+IWEEGAAIKAFK+VEKG+FQEEGI 
Sbjct: 841  PVFDKGKLVFFVASRGHHAEVGGITPGSMPPFSKAIWEEGAAIKAFKVVEKGVFQEEGIV 900

Query: 901  KLLQFPSSDEGV--IPGTRRLQDNLSDLHAQVAANHRGISLIKELIVQYGLNIVQAYMTY 960
            KLLQFPSSDE    IPGTRR+QDNLSDL AQ+AAN RGISLIKELI QYGL  VQAYM Y
Sbjct: 901  KLLQFPSSDETTTKIPGTRRIQDNLSDLQAQIAANQRGISLIKELIEQYGLGTVQAYMKY 960

Query: 961  VQLNAEEAVREMLKSVASRVSSNSAKYVEGGSIAIEEEDYMDDGSAIHLKLTIDPHKGEA 1020
            VQLNAEEAVREMLKSVA+RVSS +     G S+ IEEEDYMDDGS IHLKLTID  KGEA
Sbjct: 961  VQLNAEEAVREMLKSVANRVSSETPNSRVGNSVTIEEEDYMDDGSIIHLKLTIDADKGEA 1020

Query: 1021 NFDFSGTSPEVYGNWNAPEAVTAAAVIYCLRCMVDVDIPLNQGCLAPVKIYIPPGSFLSP 1080
            +FDF+GTSPEVYGNWNAPEAVT+AAVIYCLRC+V+VDIPLNQGCLAPV+I IP GSFLSP
Sbjct: 1021 SFDFTGTSPEVYGNWNAPEAVTSAAVIYCLRCLVNVDIPLNQGCLAPVEIRIPAGSFLSP 1080

Query: 1081 SEKAAIVGGNVLTSQRITDVILTAFQACACSQGCMNNLTFGDDTFGYYETIGGGSGAGPS 1140
            SEKAA+VGGNVLTSQR+TDV+LTAFQACACSQGCMNNLTFGDDTFGYYETIGGG GAGP+
Sbjct: 1081 SEKAAVVGGNVLTSQRVTDVVLTAFQACACSQGCMNNLTFGDDTFGYYETIGGGCGAGPT 1140

Query: 1141 WHGTSGVQCHMTNTRMTDPEIFEQRYPVLLHTFALRENSGGSGVYKGGDGLVREIEFKQP 1200
            W+GTSGVQCHMTNTRMTDPEIFEQRYPVLLH F LRENSGG+G++KGGDGLVREIEF++P
Sbjct: 1141 WNGTSGVQCHMTNTRMTDPEIFEQRYPVLLHRFGLRENSGGNGLHKGGDGLVREIEFRKP 1200

Query: 1201 VVVSILSERRVHAPRGLKGGKDGARGANFLVRKDGRRVYLGGKNTITVKAGEILQILTPG 1260
            VVVSILSERRVH+PRGL GG++G RGAN+L+ KD RR+YLGGKNT+ V+AGEILQILTPG
Sbjct: 1201 VVVSILSERRVHSPRGLNGGQNGLRGANYLITKDKRRIYLGGKNTVHVEAGEILQILTPG 1260

Query: 1261 GGGWG 1264
            GGG+G
Sbjct: 1261 GGGFG 1263