Cucsat.G5577 (gene) Cucumber (B10) v3

Overview
NameCucsat.G5577
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionXB3 in
Locationctg1296: 217578 .. 220775 (+)
RNA-Seq ExpressionCucsat.G5577
SyntenyCucsat.G5577
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CAACATTAGGAATCTAACTGCCCGAGAAATATCTGAACCCTGCCCGCAGTCACCATTTCAACTCCCTCCCTTGCTTCTACGGTCACCCTTCTCTCTCTCTTTTCCCAATTTCATCCTTCTCTGACTCCATTACCGATAGTATTTAGTAATGGTCCTAATGGACCTCTTCTTCGTATTCCATGCCACTCCCCTCCATTTCTGATCCTTTAATGGAGCTCCGAGCCTGCGTCCAACCCCTCTCTTGAGTTTCTTTCTTCTCCCTCTGTTTTCACCGTTTTAAGCAGAGGAGGAAAGATGGGGCAGGGTCTGAGTTGCAGTGCGACTCATGAGAATGGATTGTTTAGTGCTGTACAGTGTGGGGATTTAGAGATGGTTCAACTTTTGTTGAATAACCAACCATCTTTGTTGCATAATACCACTCTCTATGATCGTCAATCTCCGCTTCATTTTGCTGCTGCTAATGGGCAGATCGAGGTTTGTTGTTGTCTTTTTCTTTCTTCTTTGATTTGGGTTTCGTTTTCTTTCGTTTACCTGTTTTGGTTTATTGATTGGGGATTTTGAGTTTTGGGTTTTTTTTCTTTTTTTTTTCATTATTAGATCCTCTCTTTACTTTTGGAACGATCTGTTAATCCGGATTTGTTGAATCGTCACAAACAGGTGAGGGATTCTTCTTTTAATTTTAATTTCATCAGTTTATTTCGGAATTGTTTCTTCTTACTGGGTTTTGATTGTTTAATGTGAATGTTTGTTTTAGACTCCACTTATGTTGGCAGCAATGCATGGGAAGATCTCTTGTTTGAAGAAACTCCTTGAAGTTGGAGCAAATGTATAGAATCGATCGAATCCCATATCTTGATTCTATTCCTTTTACTTTTCACAACCAATGATTTGAAATATACTAATCTGGGTATAAATTTTTTCAGATTTTGAAGTTTGATTCTCTTCACGGGAGAACTTGCTTGCATTATGCTGCTTATTATGGCCATTTTGATTGTTTAGAAGTCATTCTTTCTGCAGCTCAATCAAGCCCCGTTGCTGCTTCCTGGTATTTGTTGCTGATCGCTCCCTTTTAACAAATACTCATTAACTTTTCATTCTAGAATCATTGGTTAACTTATTGGTCAATAGGGGATTTGTACGATTTGTGAATATTAGAGATGGTAAAGGAGCAACCCCTTTACACTTAGCAGCTCGTCAAAGACGACCTGAATGTATTCATATTCTGTTGGATAATGGAGCTCTTGTTAGTGCTTCAACGGGTGGATATGGGTATTTTTCTATATCAAGTATTCTTCAACTTTCTTTTGTTTTGAGTTGAATTTGACAAATTTCTATGAACAGATGCCAAGGAAGTACACCTCTTCATTTGGCAGCTCGTGGGGGTTCCTTGGATTGCATCAGGGAATTGTTAGCTTGGGGGGCGGATCGTCTTCAGAGAGATTCCACCGGGTATTAGAACATACTAATTTACATCATAAACTAGTTCTAATTGCATATGAATCAGGTAATTAAGGCCAGGATTATCACTTTTTCTGTGTACAGGAGAATTCCCTATGTAATTGCTATGAAGCACAGACATGGAACTTGTGCAGCCTTGCTGAATCCTTCATCCACAGAGCCACTTGTTTGGCCATCACCCTTGAAATTTATCAATGAGCTTAATCAAGAAGCAAAAGCTTTATTAGAACAGGCTCTAATGGAAGCCAACAGGGAGAGAGAGAAGAACATCTTGAAGACAAATTCTCCTGGACTACCCTCTCCATCGCATTCCGATGCAGTGGATGACAATTTGTCTGAGGTAATCAAGAATTTCCTTTGTCATTAAAGAATGTCTTTAACTTTTAAAGTGGATTTCAAACACGGCAGCCATTCGGTATTTGAAAAGTCATGACTCATGACTCTCTTTGACTCGACGTTATGAAGTTTTGCGTTTAAATCCAAAAAATTTTCGAGTTAAGAATCATAGGTAAAGAAACAAAGTTATGGGGATTGATAAGATATTGATGGTTTTGAAGGTTTTGTCCTTCCACACTATTAGTGGCATTGCTTTCCCAATTTGGAGGTATTCACTTTACGTCTGAAAGCATCTCTGATGTAATGTAGACTTGTCATTTGTTTCTTAGAGAGCTTTTTTTCAACTTAGGCTCTTAGAAGACACGTGATGAATGGTCCCAATATCTGCAATTTCAATTATCAATTCTCTTCACTTTTCTCAGATTTTTAAAAAATGATTAGCTTTTCTGCTTTAGGTCTTCGGTATGGGTCTGTCATCCCATTGATTAGTTGAGAATTTTCAAGTGCTTTTGAGATCCCTTTTATGTATTGGGAAACTTGGCTTCTTTGCAGATTAGTGATGAAGATACAGAATTATGCTCCATTTGCTTTGAACAAGCTTCCACAATTGAAGTCCAAAACTGTGGCCATGTCATGTGTGCTCAATGCACTCTTTCCCTTTGTTGCCACAGTAAACCGAACCCAACCACGGCATGCCTGACGCCACCGGTTTGCCCGTTCTGTCGAAGCAACATTCTTCATTTGGTTGTCGCAAAGACCAAAAGGGAAGAAGTAGATGACCATGAAGCTATTAACTCCAAGCAGAGAAAGGGAAGAAAGTCCCACAACTTCAGCGAAGGTGGCAGTAGCAGCTTCAAGGGGTTGTCGTCAATGACCTCTTTTGGGAAGATCGGCGGTGGCCGGAACTCTGGACGGATAGCTGCGGAAGAGAATCAGTCAGTTGATAAGCTTGGTTAATAGGTGTGGAAATGGCAAATCATACCATCTTTTTTACCAACCAAGATCCTTTTTTCCCGCATTTGCAAACAAATCAAAAAGGTTTCAATTAAACTTTTAATGTCGGCCGGGCCTCTATCAATACGTAATATAATATAGATTGGGAAAAGAAAACCAAAAAAGAAAAAAAAGAAGAAGAAAGTTGCTTGTAGAATTGGGAAATAAGAGATATTAAGAAAAGAAATGGGTTGGGTTGGTGACTTTTCATATTTGTGGGGTCATGTTTAGTTGTAATATGATTTGTTTAAATTGATATTGAAGAAGTCAAAGAAAAAAAAAAGTGAGAGATTATTTATTAAAAAAGAATGATAAATATCTAAATTCTTGAATGGATGGGGTTATTAGGACCGAGTTGAACCGGTGGTGAGTGAGACTCAAAAGGAAAACAAAAAAAAGGTTTGAAAATAT

Coding sequence (CDS)

ATGGGGCAGGGTCTGAGTTGCAGTGCGACTCATGAGAATGGATTGTTTAGTGCTGTACAGTGTGGGGATTTAGAGATGGTTCAACTTTTGTTGAATAACCAACCATCTTTGTTGCATAATACCACTCTCTATGATCGTCAATCTCCGCTTCATTTTGCTGCTGCTAATGGGCAGATCGAGATCCTCTCTTTACTTTTGGAACGATCTGTTAATCCGGATTTGTTGAATCGTCACAAACAGACTCCACTTATGTTGGCAGCAATGCATGGGAAGATCTCTTGTTTGAAGAAACTCCTTGAAGTTGGAGCAAATATTTTGAAGTTTGATTCTCTTCACGGGAGAACTTGCTTGCATTATGCTGCTTATTATGGCCATTTTGATTGTTTAGAAGTCATTCTTTCTGCAGCTCAATCAAGCCCCGTTGCTGCTTCCTGGGGATTTGTACGATTTGTGAATATTAGAGATGGTAAAGGAGCAACCCCTTTACACTTAGCAGCTCGTCAAAGACGACCTGAATGTATTCATATTCTGTTGGATAATGGAGCTCTTGTTAGTGCTTCAACGGGTGGATATGGATGCCAAGGAAGTACACCTCTTCATTTGGCAGCTCGTGGGGGTTCCTTGGATTGCATCAGGGAATTGTTAGCTTGGGGGGCGGATCGTCTTCAGAGAGATTCCACCGGGAGAATTCCCTATGTAATTGCTATGAAGCACAGACATGGAACTTGTGCAGCCTTGCTGAATCCTTCATCCACAGAGCCACTTGTTTGGCCATCACCCTTGAAATTTATCAATGAGCTTAATCAAGAAGCAAAAGCTTTATTAGAACAGGCTCTAATGGAAGCCAACAGGGAGAGAGAGAAGAACATCTTGAAGACAAATTCTCCTGGACTACCCTCTCCATCGCATTCCGATGCAGTGGATGACAATTTGTCTGAGATTAGTGATGAAGATACAGAATTATGCTCCATTTGCTTTGAACAAGCTTCCACAATTGAAGTCCAAAACTGTGGCCATGTCATGTGTGCTCAATGCACTCTTTCCCTTTGTTGCCACAGTAAACCGAACCCAACCACGGCATGCCTGACGCCACCGGTTTGCCCGTTCTGTCGAAGCAACATTCTTCATTTGGTTGTCGCAAAGACCAAAAGGGAAGAAGTAGATGACCATGAAGCTATTAACTCCAAGCAGAGAAAGGGAAGAAAGTCCCACAACTTCAGCGAAGGTGGCAGTAGCAGCTTCAAGGGGTTGTCGTCAATGACCTCTTTTGGGAAGATCGGCGGTGGCCGGAACTCTGGACGGATAGCTGCGGAAGAGAATCAGTCAGTTGATAAGCTTGGTTAA

Protein sequence

MGQGLSCSATHENGLFSAVQCGDLEMVQLLLNNQPSLLHNTTLYDRQSPLHFAAANGQIEILSLLLERSVNPDLLNRHKQTPLMLAAMHGKISCLKKLLEVGANILKFDSLHGRTCLHYAAYYGHFDCLEVILSAAQSSPVAASWGFVRFVNIRDGKGATPLHLAARQRRPECIHILLDNGALVSASTGGYGCQGSTPLHLAARGGSLDCIRELLAWGADRLQRDSTGRIPYVIAMKHRHGTCAALLNPSSTEPLVWPSPLKFINELNQEAKALLEQALMEANREREKNILKTNSPGLPSPSHSDAVDDNLSEISDEDTELCSICFEQASTIEVQNCGHVMCAQCTLSLCCHSKPNPTTACLTPPVCPFCRSNILHLVVAKTKREEVDDHEAINSKQRKGRKSHNFSEGGSSSFKGLSSMTSFGKIGGGRNSGRIAAEENQSVDKLG
Homology
BLAST of Cucsat.G5577 vs. ExPASy Swiss-Prot
Match: Q94B55 (Putative E3 ubiquitin-protein ligase XBAT31 OS=Arabidopsis thaliana OX=3702 GN=XBAT31 PE=2 SV=1)

HSP 1 Score: 609.4 bits (1570), Expect = 3.3e-173
Identity = 316/459 (68.85%), Postives = 371/459 (80.83%), Query Frame = 0

Query: 1   MGQGLSCSATHENGLFSAVQCGDLEMVQLLLNNQPSLLHNTTLYDRQSPLHFAAANGQIE 60
           MGQ +SC +  E+G+F++VQCGD+  ++ ++  +PSLL+ TT YDR S LH AAANGQIE
Sbjct: 1   MGQSMSCGSRPEHGIFASVQCGDIITIRRVMATEPSLLNQTTPYDRHSVLHVAAANGQIE 60

Query: 61  ILSLLLERSVNPDLLNRHKQTPLMLAAMHGKISCLKKLLEVGANILKFDSLHGRTCLHYA 120
           ILSLLLER  NPDLLNRHKQTPLMLAAM+G+ISC+KKL EVGANIL FDS++ RTCLHYA
Sbjct: 61  ILSLLLERFTNPDLLNRHKQTPLMLAAMYGRISCVKKLAEVGANILMFDSVNRRTCLHYA 120

Query: 121 AYYGHFDCLEVILSAAQSSPVAASWGFVRFVNIRDGKGATPLHLAARQRRPECIHILLDN 180
           AYYGH +C++ ILSAAQSSPVA  WG+ RFVNIRD KGATPLHLAARQRRPEC+++LLD+
Sbjct: 121 AYYGHANCVQAILSAAQSSPVAVHWGYARFVNIRDDKGATPLHLAARQRRPECVNVLLDS 180

Query: 181 GALVSASTGGYGCQGSTPLHLAARGGSLDCIRELLAWGADRLQRDSTGRIPYVIAMKHRH 240
           G+LV AST  YG  GSTPLHLAAR GS+DC+R+LLAWGADRLQRD++GRIPYV+AMKH+H
Sbjct: 181 GSLVCASTSVYGSPGSTPLHLAARSGSIDCVRKLLAWGADRLQRDASGRIPYVVAMKHKH 240

Query: 241 GTCAALLNPSSTEPLVWPSPLKFINELNQEAKALLEQALMEANREREKNILKTNSPGLPS 300
           G C ALLNPSS EPLVWPSPLKFI+ELN EAK LLEQALMEANREREK ILK  +  LPS
Sbjct: 241 GACGALLNPSSAEPLVWPSPLKFISELNDEAKLLLEQALMEANREREKTILKGTAYSLPS 300

Query: 301 PSHSDAVDDNLSEISDEDTELCSICFEQASTIEVQNCGHVMCAQCTLSLCCHSKPNPTTA 360
           PS SD  DDN+SE+S  DTELC ICFEQ  TIEV++CGH MCAQCTL+LCCH+KPNPTT+
Sbjct: 301 PSFSD-TDDNMSEVS--DTELCCICFEQVCTIEVKDCGHQMCAQCTLALCCHNKPNPTTS 360

Query: 361 CLTPPVCPFCRSNILHLVVAKT------KREEVDD--------HEAINSKQRKGRKSHNF 420
            +TPPVCPFCRS I  LVVA+       K + +DD         +  +SK RK R+S N 
Sbjct: 361 TVTPPVCPFCRSTIACLVVAQNNNNNNEKSKSLDDVVVVDREAGDVSSSKFRKHRRSINL 420

Query: 421 SEGGSSSFKGLSSMTSFGKIGGGRNSGRIAAEENQSVDK 446
            E  SSSF GLS++ SFG+I  GR SGRIAA EN+ +DK
Sbjct: 421 GE-ESSSFMGLSTIGSFGRI-TGRGSGRIAA-ENELMDK 453

BLAST of Cucsat.G5577 vs. ExPASy Swiss-Prot
Match: Q65XV2 (E3 ubiquitin-protein ligase XB3 OS=Oryza sativa subsp. japonica OX=39947 GN=XB3 PE=1 SV=1)

HSP 1 Score: 556.6 bits (1433), Expect = 2.6e-157
Identity = 289/450 (64.22%), Postives = 341/450 (75.78%), Query Frame = 0

Query: 1   MGQGLSCSAT-HENGLFSAVQCGDLEMVQLLLNNQPSLLHNTTLYDRQSPLHFAAANGQI 60
           MG G+SC+ T  E+  F A Q GDL+ +  LL   PSL    TLYDR S LH AAANG+I
Sbjct: 1   MGHGVSCARTGDEHDFFRAAQLGDLDALAALLAADPSLARRATLYDRLSVLHIAAANGRI 60

Query: 61  EILSLLLERSVNPDLLNRHKQTPLMLAAMHGKISCLKKLLEVGANILKFDSLHGRTCLHY 120
           E+LS+ L+R   PD +NRHKQTPLMLAAMHGKI C+ KLL+  ANIL FDS+H RTCLH+
Sbjct: 61  EVLSMFLDRGAPPDAVNRHKQTPLMLAAMHGKIDCVLKLLQADANILMFDSVHARTCLHH 120

Query: 121 AAYYGHFDCLEVILSAAQSSPVAASWGFVRFVNIRDGKGATPLHLAARQRRPECIHILLD 180
           AAYYGH DCL+ IL+AAQ++PVA SWGF RFVN+RD  GATPLHLAARQ RP C+ +LL+
Sbjct: 121 AAYYGHVDCLQAILAAAQTTPVADSWGFARFVNVRDDHGATPLHLAARQGRPGCVQVLLE 180

Query: 181 NGALVSASTGGYGCQGSTPLHLAARGGSLDCIRELLAWGADRLQRDSTGRIPYVIAMKHR 240
           NGA+VSA TG YG  GST LHLAAR G+LDCIR+LLAWGADRLQRDS GRIPY +A+K  
Sbjct: 181 NGAIVSALTGSYGFPGSTSLHLAARSGNLDCIRKLLAWGADRLQRDSAGRIPYSVALKRN 240

Query: 241 HGTCAALLNPSSTEPLVWPSPLKFINELNQEAKALLEQALMEANREREKNILKTNSPGLP 300
           HG CAALLNP+S EP+VWPSPLKFI+EL  EAKALLE ALMEANREREK IL      LP
Sbjct: 241 HGACAALLNPTSAEPMVWPSPLKFISELEPEAKALLEAALMEANREREKKILNGTKYSLP 300

Query: 301 SPSHSD--AVDDNLSEISDEDTELCSICFEQASTIEVQNCGHVMCAQCTLSLCCHSKPNP 360
           SPS  D  A DD  SE+S  DTELC ICF+QA TIEVQ+CGH MCA CTL+LCCH+KPNP
Sbjct: 301 SPSPGDDSADDDACSEVS--DTELCCICFDQACTIEVQDCGHQMCAPCTLALCCHNKPNP 360

Query: 361 TTACLTPPVCPFCRSNILHLVVAKTKRE-EVDDHEAINSKQRKGRKSHNFSEGGSSSFKG 420
           TT     P CPFCR +I  LVVA+T+   + D   ++   +++ R+SHN SE GSSSFKG
Sbjct: 361 TTLTPPSPACPFCRGSISRLVVAQTRSACDPDKPSSLQLTRKRSRRSHNLSE-GSSSFKG 420

Query: 421 L-SSMTSFGKIGGGRNSGRIAAEENQSVDK 446
           L S+M SF K+  GR S R+A  ++ ++DK
Sbjct: 421 LPSAMGSFSKL--GRGSSRMADSDSSNLDK 445

BLAST of Cucsat.G5577 vs. ExPASy Swiss-Prot
Match: Q94CT7 (Probable E3 ubiquitin-protein ligase XBOS31 OS=Oryza sativa subsp. japonica OX=39947 GN=XBOS31 PE=2 SV=1)

HSP 1 Score: 468.8 bits (1205), Expect = 7.0e-131
Identity = 250/443 (56.43%), Postives = 304/443 (68.62%), Query Frame = 0

Query: 1   MGQGLSCSA-THENGLFSAVQCGDLEMVQLLLNNQPSLLHNTTLYDRQSPLHFAAANGQI 60
           MG GLSCS  T E  LF A Q GD+  +  LL   P+L    T+YDR + LH AAANG++
Sbjct: 1   MGHGLSCSRDTDEYDLFRAAQLGDIHALSALLAADPALARRATVYDRFTALHIAAANGRL 60

Query: 61  EILSLLLERSVNPDLLNRHKQTPLMLAAMHGKISCLKKLLEVGANILKFDSLHGRTCLHY 120
           ++LS+LL+R  + D+L+R KQTPLM+AAM G   C+ +LL  GAN+L FDS   RTCLH+
Sbjct: 61  QVLSMLLDRDGDVDVLSRKKQTPLMVAAMRGNTECVVRLLRGGANVLTFDSPRARTCLHH 120

Query: 121 AAYYGHFDCLEVILSAAQSS--PVAASWGFVRFVNIRDGKGATPLHLAARQRRPECIHIL 180
           AAYYGH +CL+ IL AA  +  PVAASWGF RFVN+RD +GATPLHLAAR  R  C+ +L
Sbjct: 121 AAYYGHAECLQAILGAAAQAQGPVAASWGFARFVNVRDERGATPLHLAARHARASCVRLL 180

Query: 181 LDNGALVSASTGGYGCQGSTPLHLAARGGSLDCIRELLAWGADRLQRDSTGRIPYVIAMK 240
           LD GA+VSA T  YG  GST LHLAAR GS++CIRELLAWGADRLQRDS GRI Y +AM+
Sbjct: 181 LDKGAIVSAPTAVYGFPGSTALHLAARAGSMECIRELLAWGADRLQRDSAGRIAYAVAMR 240

Query: 241 HRHGTCAALLNPSSTEPLVWPSPLKFINELNQEAKALLEQALMEANREREKNILKTNSPG 300
             H  CAALLNP++ EP+VWPSPLKFI EL  +AKALLE ALMEANREREK IL  +   
Sbjct: 241 RGHRACAALLNPAAAEPIVWPSPLKFIGELEADAKALLEAALMEANREREKRILHGSDIN 300

Query: 301 LPSPSHSDAVDDNLSEISDEDTELCSICFEQASTIEVQNCGHVMCAQCTLSLCCHSKPNP 360
           +              E S+++ E C+ICFEQA ++EV+ CGH MCA CTL++CCHSKPNP
Sbjct: 301 IKGGDE--------EEESEDEEEACNICFEQACSMEVKECGHQMCAACTLAICCHSKPNP 360

Query: 361 TTACLTPPVCPFCRSNILHLVVAKTKREEVDDHEAINSKQRKGRKSHNFSEGGSSSFKG- 420
            T  L PP CPFCR+ I  LVVA T   +       NS++R        S   SSSFKG 
Sbjct: 361 KTLLLHPPACPFCRTTISRLVVATTNSNKT------NSRRR--------SRSRSSSFKGG 419

Query: 421 -LSSMTSFGKIGGGRNSGRIAAE 439
             S+M SF +I  GR SGR+  +
Sbjct: 421 LSSAMGSFSRI--GRGSGRLVVD 419

BLAST of Cucsat.G5577 vs. ExPASy Swiss-Prot
Match: Q337A0 (Probable E3 ubiquitin-protein ligase XBOS33 OS=Oryza sativa subsp. japonica OX=39947 GN=XBOS33 PE=2 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 7.9e-42
Identity = 129/413 (31.23%), Postives = 193/413 (46.73%), Query Frame = 0

Query: 1   MGQGLSCSATHENGLFSAVQCGDLEMVQLLLNNQPSLLHNTTLYDRQSPLHFAAANGQIE 60
           MG  L CSA+ E  L SA + GD    ++LL   P+L   +T     SPLHFAAA G ++
Sbjct: 1   MGNSLGCSASGER-LVSAARDGDAVEARMLLELSPALARYSTFGGLNSPLHFAAAKGHLD 60

Query: 61  ILSLLLERSVNPDLLNRHKQTPLMLAAMHGKISCLKKLLEVGANILKFDSLHGRTCLHYA 120
           I++LLLE+  + ++ N   QT LM A  HG    ++ LL    N+ + D L GRT LH+A
Sbjct: 61  IVTLLLEKGADVNVRNYCGQTALMHACRHGHWEVVQMLLLFRCNVTRADYLSGRTALHFA 120

Query: 121 AYYGHFDCLEVILS----------------------AAQSSPVAASWGF-------VRFV 180
           A+ G   C+ ++L+                          S   +S G        +R++
Sbjct: 121 AHDGLVRCVRLLLADFVPSAPLEDGASSTVDGGECQTNSGSSPCSSLGLKFNESARLRYI 180

Query: 181 NIRDGKGATPLHLAARQRRPECIHILLDNGALVSASTGGYGC------QGSTPLHLAARG 240
           N     G T LH+AA     +C+ +L+D GA VSA T  YG        GSTPLH AA G
Sbjct: 181 NKPADGGVTALHMAALNGHFDCMQLLIDLGANVSAVTFPYGTTANLIGAGSTPLHYAAGG 240

Query: 241 GSLDCIRELLAWGADRLQRDSTGRIPYVIAMKHRHGTCAALLNPSSTEPLVWPSPLKFIN 300
           G+ +C + LL+ GA +L  +  G +P  +A          LL+P+S   +    P  ++ 
Sbjct: 241 GNAECCQLLLSKGASKLTLNCNGWLPIDVARMFGRRFLEPLLSPNSNSSIPAYQPSNYL- 300

Query: 301 ELNQEAKALLEQALMEANREREKNILKTNSPGLPSPSHSDAVDDNLSEISDEDTELCSIC 360
                  AL   +++  N  RE  +L T +                   S +D++LC++C
Sbjct: 301 -------ALPFMSIL--NIAREFGLLHTVA-------------------SVDDSDLCAVC 360

Query: 361 FEQASTIEVQNCGHVMCAQCTLSLCCHSKPNPTTACLTPP---VCPFCRSNIL 376
            E++ ++  + C H  C +C L LC  S  N       PP    CP CR+ I+
Sbjct: 361 LERSCSVAAEGCCHEFCIKCALYLC--STSNTRVEFTGPPGSIPCPLCRNGIM 381

BLAST of Cucsat.G5577 vs. ExPASy Swiss-Prot
Match: Q4FE45 (E3 ubiquitin-protein ligase XBAT33 OS=Arabidopsis thaliana OX=3702 GN=XBAT33 PE=2 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 8.8e-41
Identity = 136/398 (34.17%), Postives = 185/398 (46.48%), Query Frame = 0

Query: 1   MGQGLSCSATHENGLFSAVQCGDLEMVQLLLNNQPSLLHNTTLYDRQSPLHFAAANGQIE 60
           MG    CSA+ E  L SA + GD    ++LL+  P L   +T     SPLHFAAA G  E
Sbjct: 1   MGNSFGCSASGER-LVSAARDGDFVEAKMLLDCNPCLAKYSTFGGLNSPLHFAAAKGHNE 60

Query: 61  ILSLLLERSVNPDLLNRHKQTPLMLAAMHGKISCLKKLLEVGANILKFDSLHGRTCLHYA 120
           I+ LLLE   + +  N   QT LM A  +G    ++ LL    N+ + D L GRT LH+A
Sbjct: 61  IVGLLLENGADVNSRNYCGQTALMQACRYGHWEVVQTLLLFRCNVTRADYLAGRTALHFA 120

Query: 121 AYYGHFDCLEVIL----------SAAQSSPVAA-----SWGFVRFVNIRDGKGATPLHLA 180
           A  GH  C+ ++L          S  ++  V A          +FVN     G T LH+A
Sbjct: 121 AVNGHARCIRLVLADFLPSDKLNSLPETGVVTAKNKSEQSALSKFVNKAADGGITALHMA 180

Query: 181 ARQRRPECIHILLDNGALVSASTGGYGCQ------GSTPLHLAARGGSLDCIRELLAWGA 240
           A     +C+ +LLD  A VSA T  YG        GSTPLH AA GG+L C + LLA GA
Sbjct: 181 ALNGLFDCVQLLLDLEANVSAVTFHYGTSMDMIGAGSTPLHYAACGGNLKCCQILLARGA 240

Query: 241 DRLQRDSTGRIPYVIAMKHRHGTCAALLNPSSTEPLVWPSPLKFINELNQEAKALLEQAL 300
            ++  +  G +P  IA          LL+P+S   +V P+     N L+    ++L    
Sbjct: 241 RKMTLNCNGWLPIDIARMWSRHWLEPLLSPNS--DVVIPA-FPHSNYLSLPLLSIL---- 300

Query: 301 MEANREREKNILKTNSPGLPSPSHSDAVDDNLSEISDEDTELCSICFEQASTIEVQNCGH 360
              N  RE         GL S +  D VD            +C++C E+  T+  + C H
Sbjct: 301 ---NIAREF--------GLQSATIGDEVD------------ICAVCLERTCTVAAEGCEH 360

Query: 361 VMCAQCTLSLCCHSKPNPTTACLTPP---VCPFCRSNI 375
            +C +C L LC  S  N  +  + PP    CP CR  I
Sbjct: 361 QLCVRCALYLC--SSSNVPSVTVGPPGSIPCPLCRHGI 365

BLAST of Cucsat.G5577 vs. NCBI nr
Match: XP_004143108.1 (putative E3 ubiquitin-protein ligase XBAT31 [Cucumis sativus] >KGN47159.1 hypothetical protein Csa_021018 [Cucumis sativus])

HSP 1 Score: 899 bits (2322), Expect = 0.0
Identity = 447/447 (100.00%), Postives = 447/447 (100.00%), Query Frame = 0

Query: 1   MGQGLSCSATHENGLFSAVQCGDLEMVQLLLNNQPSLLHNTTLYDRQSPLHFAAANGQIE 60
           MGQGLSCSATHENGLFSAVQCGDLEMVQLLLNNQPSLLHNTTLYDRQSPLHFAAANGQIE
Sbjct: 1   MGQGLSCSATHENGLFSAVQCGDLEMVQLLLNNQPSLLHNTTLYDRQSPLHFAAANGQIE 60

Query: 61  ILSLLLERSVNPDLLNRHKQTPLMLAAMHGKISCLKKLLEVGANILKFDSLHGRTCLHYA 120
           ILSLLLERSVNPDLLNRHKQTPLMLAAMHGKISCLKKLLEVGANILKFDSLHGRTCLHYA
Sbjct: 61  ILSLLLERSVNPDLLNRHKQTPLMLAAMHGKISCLKKLLEVGANILKFDSLHGRTCLHYA 120

Query: 121 AYYGHFDCLEVILSAAQSSPVAASWGFVRFVNIRDGKGATPLHLAARQRRPECIHILLDN 180
           AYYGHFDCLEVILSAAQSSPVAASWGFVRFVNIRDGKGATPLHLAARQRRPECIHILLDN
Sbjct: 121 AYYGHFDCLEVILSAAQSSPVAASWGFVRFVNIRDGKGATPLHLAARQRRPECIHILLDN 180

Query: 181 GALVSASTGGYGCQGSTPLHLAARGGSLDCIRELLAWGADRLQRDSTGRIPYVIAMKHRH 240
           GALVSASTGGYGCQGSTPLHLAARGGSLDCIRELLAWGADRLQRDSTGRIPYVIAMKHRH
Sbjct: 181 GALVSASTGGYGCQGSTPLHLAARGGSLDCIRELLAWGADRLQRDSTGRIPYVIAMKHRH 240

Query: 241 GTCAALLNPSSTEPLVWPSPLKFINELNQEAKALLEQALMEANREREKNILKTNSPGLPS 300
           GTCAALLNPSSTEPLVWPSPLKFINELNQEAKALLEQALMEANREREKNILKTNSPGLPS
Sbjct: 241 GTCAALLNPSSTEPLVWPSPLKFINELNQEAKALLEQALMEANREREKNILKTNSPGLPS 300

Query: 301 PSHSDAVDDNLSEISDEDTELCSICFEQASTIEVQNCGHVMCAQCTLSLCCHSKPNPTTA 360
           PSHSDAVDDNLSEISDEDTELCSICFEQASTIEVQNCGHVMCAQCTLSLCCHSKPNPTTA
Sbjct: 301 PSHSDAVDDNLSEISDEDTELCSICFEQASTIEVQNCGHVMCAQCTLSLCCHSKPNPTTA 360

Query: 361 CLTPPVCPFCRSNILHLVVAKTKREEVDDHEAINSKQRKGRKSHNFSEGGSSSFKGLSSM 420
           CLTPPVCPFCRSNILHLVVAKTKREEVDDHEAINSKQRKGRKSHNFSEGGSSSFKGLSSM
Sbjct: 361 CLTPPVCPFCRSNILHLVVAKTKREEVDDHEAINSKQRKGRKSHNFSEGGSSSFKGLSSM 420

Query: 421 TSFGKIGGGRNSGRIAAEENQSVDKLG 447
           TSFGKIGGGRNSGRIAAEENQSVDKLG
Sbjct: 421 TSFGKIGGGRNSGRIAAEENQSVDKLG 447

BLAST of Cucsat.G5577 vs. NCBI nr
Match: XP_008464140.1 (PREDICTED: putative E3 ubiquitin-protein ligase XBAT31 [Cucumis melo])

HSP 1 Score: 890 bits (2299), Expect = 0.0
Identity = 441/447 (98.66%), Postives = 445/447 (99.55%), Query Frame = 0

Query: 1   MGQGLSCSATHENGLFSAVQCGDLEMVQLLLNNQPSLLHNTTLYDRQSPLHFAAANGQIE 60
           MGQGLSCS++HENGLF+AVQCGDLEMVQLLLNNQPSLLHNTTLYDRQSPLHFAAANGQIE
Sbjct: 1   MGQGLSCSSSHENGLFNAVQCGDLEMVQLLLNNQPSLLHNTTLYDRQSPLHFAAANGQIE 60

Query: 61  ILSLLLERSVNPDLLNRHKQTPLMLAAMHGKISCLKKLLEVGANILKFDSLHGRTCLHYA 120
           ILSLLLERSVNPDLLNRHKQTPLMLAAMHGKISCLKKLLEVGANILKFDSLHGRTCLHYA
Sbjct: 61  ILSLLLERSVNPDLLNRHKQTPLMLAAMHGKISCLKKLLEVGANILKFDSLHGRTCLHYA 120

Query: 121 AYYGHFDCLEVILSAAQSSPVAASWGFVRFVNIRDGKGATPLHLAARQRRPECIHILLDN 180
           AYYGHFDCLE ILSAAQSSPVA SWGFVRFVNIRDGKGATPLHLAARQRRPECIHILLDN
Sbjct: 121 AYYGHFDCLEAILSAAQSSPVAVSWGFVRFVNIRDGKGATPLHLAARQRRPECIHILLDN 180

Query: 181 GALVSASTGGYGCQGSTPLHLAARGGSLDCIRELLAWGADRLQRDSTGRIPYVIAMKHRH 240
           GALVSASTGGYGCQGSTPLHLAARGGSLDCIRELLAWGADRLQRDSTGRIPYVIAMKHRH
Sbjct: 181 GALVSASTGGYGCQGSTPLHLAARGGSLDCIRELLAWGADRLQRDSTGRIPYVIAMKHRH 240

Query: 241 GTCAALLNPSSTEPLVWPSPLKFINELNQEAKALLEQALMEANREREKNILKTNSPGLPS 300
           GTCAALLNPSSTEPLVWPSPLKFINELNQEAKALLEQALMEANREREKNILKTNSPGLPS
Sbjct: 241 GTCAALLNPSSTEPLVWPSPLKFINELNQEAKALLEQALMEANREREKNILKTNSPGLPS 300

Query: 301 PSHSDAVDDNLSEISDEDTELCSICFEQASTIEVQNCGHVMCAQCTLSLCCHSKPNPTTA 360
           PSHSDAVDDNLSEISDEDTELCSICFEQASTIEVQNCGHVMCAQCTLSLCCHSKPNPTTA
Sbjct: 301 PSHSDAVDDNLSEISDEDTELCSICFEQASTIEVQNCGHVMCAQCTLSLCCHSKPNPTTA 360

Query: 361 CLTPPVCPFCRSNILHLVVAKTKREEVDDHEAINSKQRKGRKSHNFSEGGSSSFKGLSSM 420
           CLTPPVCPFCRSNILHLVVAKTKREEVDDHEAINSKQRKGRKSHNFSEGGSSSFKGLSSM
Sbjct: 361 CLTPPVCPFCRSNILHLVVAKTKREEVDDHEAINSKQRKGRKSHNFSEGGSSSFKGLSSM 420

Query: 421 TSFGKIGGGRNSGRIAAEENQSVDKLG 447
           TSFGKIGGGRNSGRIAAEE+QSVDKLG
Sbjct: 421 TSFGKIGGGRNSGRIAAEEDQSVDKLG 447

BLAST of Cucsat.G5577 vs. NCBI nr
Match: XP_038902234.1 (putative E3 ubiquitin-protein ligase XBAT31 [Benincasa hispida])

HSP 1 Score: 865 bits (2236), Expect = 0.0
Identity = 433/448 (96.65%), Postives = 438/448 (97.77%), Query Frame = 0

Query: 1   MGQGLSCSATHENGLFSAVQCGDLEMVQLLLNNQPSLLHNTTLYDRQSPLHFAAANGQIE 60
           MGQGLSCSA+HENGLFSAVQCGDLEMVQ+LL N PSLLHNTTLYDRQSPLHFAAANGQIE
Sbjct: 1   MGQGLSCSASHENGLFSAVQCGDLEMVQVLLKNDPSLLHNTTLYDRQSPLHFAAANGQIE 60

Query: 61  ILSLLLERSVNPDLLNRHKQTPLMLAAMHGKISCLKKLLEVGANILKFDSLHGRTCLHYA 120
           ILSLLLERSVNPDLLNRHKQTPLMLAAMHGKISCLKKLLEVGANILKFDSLHGRTCLHYA
Sbjct: 61  ILSLLLERSVNPDLLNRHKQTPLMLAAMHGKISCLKKLLEVGANILKFDSLHGRTCLHYA 120

Query: 121 AYYGHFDCLEVILSAAQSSPVAASWGFVRFVNIRDGKGATPLHLAARQRRPECIHILLDN 180
           AYYGHFDCLE ILSAAQSSPVAASWGFVRFVNIRDGKGATPLHLAARQRRPECIHILLDN
Sbjct: 121 AYYGHFDCLEAILSAAQSSPVAASWGFVRFVNIRDGKGATPLHLAARQRRPECIHILLDN 180

Query: 181 GALVSASTGGYGCQGSTPLHLAARGGSLDCIRELLAWGADRLQRDSTGRIPYVIAMKHRH 240
           GALVSASTGGYGCQGSTPLHLAARGGSLDCIRELLAWGADRLQRDSTGRIPYVIAMKHRH
Sbjct: 181 GALVSASTGGYGCQGSTPLHLAARGGSLDCIRELLAWGADRLQRDSTGRIPYVIAMKHRH 240

Query: 241 GTCAALLNPSSTEPLVWPSPLKFINELNQEAKALLEQALMEANREREKNILKTNSPGLPS 300
           GTCAALLNPSSTEPLVWPSPLKFINELNQEAKALLEQALMEANREREK+ILKTNS GLPS
Sbjct: 241 GTCAALLNPSSTEPLVWPSPLKFINELNQEAKALLEQALMEANREREKSILKTNSHGLPS 300

Query: 301 PSHSDAVDDNLSEISDEDTELCSICFEQASTIEVQNCGHVMCAQCTLSLCCHSKPNPTTA 360
           PSHSDAVDDNLSEISD D ELCSICFEQASTIEVQNCGHVMCAQCTLSLCCHSKPNPTTA
Sbjct: 301 PSHSDAVDDNLSEISDGDAELCSICFEQASTIEVQNCGHVMCAQCTLSLCCHSKPNPTTA 360

Query: 361 CLTPPVCPFCRSNILHLVVAKTK-REEVDDHEAINSKQRKGRKSHNFSEGGSSSFKGLSS 420
           CL PPVCPFCRSNILHLVVAKTK REEVDDHE  NSKQRKGRKSHNFSEGGSSSFKGLSS
Sbjct: 361 CLMPPVCPFCRSNILHLVVAKTKTREEVDDHETNNSKQRKGRKSHNFSEGGSSSFKGLSS 420

Query: 421 MTSFGKIGGGRNSGRIAAEENQSVDKLG 447
           M+SFGKIGGGRNSGRIAAEE+QSVDKLG
Sbjct: 421 MSSFGKIGGGRNSGRIAAEEDQSVDKLG 448

BLAST of Cucsat.G5577 vs. NCBI nr
Match: KAG6570711.1 (putative E3 ubiquitin-protein ligase XBAT31, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 823 bits (2127), Expect = 8.38e-300
Identity = 411/448 (91.74%), Postives = 427/448 (95.31%), Query Frame = 0

Query: 1   MGQGLSCSATHENGLFSAVQCGDLEMVQLLLNNQPSLLHNTTLYDRQSPLHFAAANGQIE 60
           MGQGLSCSA+HENGLFSAVQCGDL MV++ L N+PSLL NTTLYDRQSPLH AAANGQIE
Sbjct: 1   MGQGLSCSASHENGLFSAVQCGDLAMVEVFLKNKPSLLLNTTLYDRQSPLHIAAANGQIE 60

Query: 61  ILSLLLERSVNPDLLNRHKQTPLMLAAMHGKISCLKKLLEVGANILKFDSLHGRTCLHYA 120
           ILSLLLERSVNPDLLNRHKQTPLMLAAMHGKISCL+KLLEVGANILKFDSL+GRTCLHYA
Sbjct: 61  ILSLLLERSVNPDLLNRHKQTPLMLAAMHGKISCLRKLLEVGANILKFDSLNGRTCLHYA 120

Query: 121 AYYGHFDCLEVILSAAQSSPVAASWGFVRFVNIRDGKGATPLHLAARQRRPECIHILLDN 180
           AYYGHFDCLE ILSAAQSSPVAASWGFVRFVNIRDGKGATPLHLAAR+RRPECIHILLDN
Sbjct: 121 AYYGHFDCLEAILSAAQSSPVAASWGFVRFVNIRDGKGATPLHLAARRRRPECIHILLDN 180

Query: 181 GALVSASTGGYGCQGSTPLHLAARGGSLDCIRELLAWGADRLQRDSTGRIPYVIAMKHRH 240
           GALV ASTGGYGCQGSTPLHLAARGGSLDCIRELLAWGADRLQRDS+GRIPYVIAMKH+H
Sbjct: 181 GALVGASTGGYGCQGSTPLHLAARGGSLDCIRELLAWGADRLQRDSSGRIPYVIAMKHKH 240

Query: 241 GTCAALLNPSSTEPLVWPSPLKFINELNQEAKALLEQALMEANREREKNILKTNSPGLPS 300
           GTCAALLNPSSTEPLVWPSPLKFINELNQEAKALLEQALMEANREREK+ILKTNS    S
Sbjct: 241 GTCAALLNPSSTEPLVWPSPLKFINELNQEAKALLEQALMEANREREKSILKTNSNEFSS 300

Query: 301 PSHSDAVDDNLSEISDEDTELCSICFEQASTIEVQNCGHVMCAQCTLSLCCHSKPNPTTA 360
           P+HSDAVDD+LSEISD D ELCSICFEQ STIEVQNCGHVMCAQCTLSLCCHSKPNP TA
Sbjct: 301 PAHSDAVDDSLSEISDGDAELCSICFEQPSTIEVQNCGHVMCAQCTLSLCCHSKPNPATA 360

Query: 361 CLTPPVCPFCRSNILHLVVAKTK-REEVDDHEAINSKQRKGRKSHNFSEGGSSSFKGLSS 420
           CLTPPVCPFCRSNI+HLVV KTK +EEVDDHE  NSKQRKGRKSHNFSEGGSSSFKGLSS
Sbjct: 361 CLTPPVCPFCRSNIIHLVVKKTKTKEEVDDHETNNSKQRKGRKSHNFSEGGSSSFKGLSS 420

Query: 421 MTSFGKIGGGRNSGRIAAEENQSVDKLG 447
           MTSFGKI GGR+SGRIAAEE+Q VDKLG
Sbjct: 421 MTSFGKINGGRSSGRIAAEEDQFVDKLG 448

BLAST of Cucsat.G5577 vs. NCBI nr
Match: XP_023511973.1 (putative E3 ubiquitin-protein ligase XBAT31 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 822 bits (2123), Expect = 3.41e-299
Identity = 410/448 (91.52%), Postives = 425/448 (94.87%), Query Frame = 0

Query: 1   MGQGLSCSATHENGLFSAVQCGDLEMVQLLLNNQPSLLHNTTLYDRQSPLHFAAANGQIE 60
           MGQGLSC A+HENGLFSAVQCGDL MV++ L N+PSLL NTTLYDRQSPLH AAANGQIE
Sbjct: 1   MGQGLSCGASHENGLFSAVQCGDLAMVEVFLKNKPSLLLNTTLYDRQSPLHIAAANGQIE 60

Query: 61  ILSLLLERSVNPDLLNRHKQTPLMLAAMHGKISCLKKLLEVGANILKFDSLHGRTCLHYA 120
           ILSLLLERSVNPDLLNRHKQTPLMLAAMHGKISCL+KLLEVGANILKFDSL+GRTCLHYA
Sbjct: 61  ILSLLLERSVNPDLLNRHKQTPLMLAAMHGKISCLRKLLEVGANILKFDSLNGRTCLHYA 120

Query: 121 AYYGHFDCLEVILSAAQSSPVAASWGFVRFVNIRDGKGATPLHLAARQRRPECIHILLDN 180
           AYYGHFDCLE ILSAAQSSPVAASWGFVRFVNIRDGKGATPLHLAARQRRPECIH LLDN
Sbjct: 121 AYYGHFDCLEAILSAAQSSPVAASWGFVRFVNIRDGKGATPLHLAARQRRPECIHFLLDN 180

Query: 181 GALVSASTGGYGCQGSTPLHLAARGGSLDCIRELLAWGADRLQRDSTGRIPYVIAMKHRH 240
           GALV ASTGGYGCQGSTPLHLAARGGSLDCIRELLAWGADRLQRDS+GRIPYVIAMKH+H
Sbjct: 181 GALVGASTGGYGCQGSTPLHLAARGGSLDCIRELLAWGADRLQRDSSGRIPYVIAMKHKH 240

Query: 241 GTCAALLNPSSTEPLVWPSPLKFINELNQEAKALLEQALMEANREREKNILKTNSPGLPS 300
           GTCAALLNPSSTEPLVWPSPLKFINELNQEAKALLEQALMEANREREK+ILKTNS    S
Sbjct: 241 GTCAALLNPSSTEPLVWPSPLKFINELNQEAKALLEQALMEANREREKSILKTNSHEFSS 300

Query: 301 PSHSDAVDDNLSEISDEDTELCSICFEQASTIEVQNCGHVMCAQCTLSLCCHSKPNPTTA 360
           PSHSDAVDD+LSEISD D ELCSICFEQ STIEV+NCGHVMCAQCTLSLCCHSKPNP TA
Sbjct: 301 PSHSDAVDDSLSEISDGDAELCSICFEQPSTIEVENCGHVMCAQCTLSLCCHSKPNPATA 360

Query: 361 CLTPPVCPFCRSNILHLVVAKTK-REEVDDHEAINSKQRKGRKSHNFSEGGSSSFKGLSS 420
           CLTPPVCPFCRSNI+HLVV KTK +EEVDDHE  NSKQRKGRKSHNFSEGGSSSFKGLSS
Sbjct: 361 CLTPPVCPFCRSNIIHLVVKKTKTKEEVDDHETNNSKQRKGRKSHNFSEGGSSSFKGLSS 420

Query: 421 MTSFGKIGGGRNSGRIAAEENQSVDKLG 447
           MTSFGKI GGR+SGRIAAEE+Q VDKLG
Sbjct: 421 MTSFGKISGGRSSGRIAAEEDQFVDKLG 448

BLAST of Cucsat.G5577 vs. ExPASy TrEMBL
Match: A0A0A0KE96 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G190990 PE=4 SV=1)

HSP 1 Score: 899 bits (2322), Expect = 0.0
Identity = 447/447 (100.00%), Postives = 447/447 (100.00%), Query Frame = 0

Query: 1   MGQGLSCSATHENGLFSAVQCGDLEMVQLLLNNQPSLLHNTTLYDRQSPLHFAAANGQIE 60
           MGQGLSCSATHENGLFSAVQCGDLEMVQLLLNNQPSLLHNTTLYDRQSPLHFAAANGQIE
Sbjct: 1   MGQGLSCSATHENGLFSAVQCGDLEMVQLLLNNQPSLLHNTTLYDRQSPLHFAAANGQIE 60

Query: 61  ILSLLLERSVNPDLLNRHKQTPLMLAAMHGKISCLKKLLEVGANILKFDSLHGRTCLHYA 120
           ILSLLLERSVNPDLLNRHKQTPLMLAAMHGKISCLKKLLEVGANILKFDSLHGRTCLHYA
Sbjct: 61  ILSLLLERSVNPDLLNRHKQTPLMLAAMHGKISCLKKLLEVGANILKFDSLHGRTCLHYA 120

Query: 121 AYYGHFDCLEVILSAAQSSPVAASWGFVRFVNIRDGKGATPLHLAARQRRPECIHILLDN 180
           AYYGHFDCLEVILSAAQSSPVAASWGFVRFVNIRDGKGATPLHLAARQRRPECIHILLDN
Sbjct: 121 AYYGHFDCLEVILSAAQSSPVAASWGFVRFVNIRDGKGATPLHLAARQRRPECIHILLDN 180

Query: 181 GALVSASTGGYGCQGSTPLHLAARGGSLDCIRELLAWGADRLQRDSTGRIPYVIAMKHRH 240
           GALVSASTGGYGCQGSTPLHLAARGGSLDCIRELLAWGADRLQRDSTGRIPYVIAMKHRH
Sbjct: 181 GALVSASTGGYGCQGSTPLHLAARGGSLDCIRELLAWGADRLQRDSTGRIPYVIAMKHRH 240

Query: 241 GTCAALLNPSSTEPLVWPSPLKFINELNQEAKALLEQALMEANREREKNILKTNSPGLPS 300
           GTCAALLNPSSTEPLVWPSPLKFINELNQEAKALLEQALMEANREREKNILKTNSPGLPS
Sbjct: 241 GTCAALLNPSSTEPLVWPSPLKFINELNQEAKALLEQALMEANREREKNILKTNSPGLPS 300

Query: 301 PSHSDAVDDNLSEISDEDTELCSICFEQASTIEVQNCGHVMCAQCTLSLCCHSKPNPTTA 360
           PSHSDAVDDNLSEISDEDTELCSICFEQASTIEVQNCGHVMCAQCTLSLCCHSKPNPTTA
Sbjct: 301 PSHSDAVDDNLSEISDEDTELCSICFEQASTIEVQNCGHVMCAQCTLSLCCHSKPNPTTA 360

Query: 361 CLTPPVCPFCRSNILHLVVAKTKREEVDDHEAINSKQRKGRKSHNFSEGGSSSFKGLSSM 420
           CLTPPVCPFCRSNILHLVVAKTKREEVDDHEAINSKQRKGRKSHNFSEGGSSSFKGLSSM
Sbjct: 361 CLTPPVCPFCRSNILHLVVAKTKREEVDDHEAINSKQRKGRKSHNFSEGGSSSFKGLSSM 420

Query: 421 TSFGKIGGGRNSGRIAAEENQSVDKLG 447
           TSFGKIGGGRNSGRIAAEENQSVDKLG
Sbjct: 421 TSFGKIGGGRNSGRIAAEENQSVDKLG 447

BLAST of Cucsat.G5577 vs. ExPASy TrEMBL
Match: A0A1S3CKT3 (putative E3 ubiquitin-protein ligase XBAT31 OS=Cucumis melo OX=3656 GN=LOC103502091 PE=4 SV=1)

HSP 1 Score: 890 bits (2299), Expect = 0.0
Identity = 441/447 (98.66%), Postives = 445/447 (99.55%), Query Frame = 0

Query: 1   MGQGLSCSATHENGLFSAVQCGDLEMVQLLLNNQPSLLHNTTLYDRQSPLHFAAANGQIE 60
           MGQGLSCS++HENGLF+AVQCGDLEMVQLLLNNQPSLLHNTTLYDRQSPLHFAAANGQIE
Sbjct: 1   MGQGLSCSSSHENGLFNAVQCGDLEMVQLLLNNQPSLLHNTTLYDRQSPLHFAAANGQIE 60

Query: 61  ILSLLLERSVNPDLLNRHKQTPLMLAAMHGKISCLKKLLEVGANILKFDSLHGRTCLHYA 120
           ILSLLLERSVNPDLLNRHKQTPLMLAAMHGKISCLKKLLEVGANILKFDSLHGRTCLHYA
Sbjct: 61  ILSLLLERSVNPDLLNRHKQTPLMLAAMHGKISCLKKLLEVGANILKFDSLHGRTCLHYA 120

Query: 121 AYYGHFDCLEVILSAAQSSPVAASWGFVRFVNIRDGKGATPLHLAARQRRPECIHILLDN 180
           AYYGHFDCLE ILSAAQSSPVA SWGFVRFVNIRDGKGATPLHLAARQRRPECIHILLDN
Sbjct: 121 AYYGHFDCLEAILSAAQSSPVAVSWGFVRFVNIRDGKGATPLHLAARQRRPECIHILLDN 180

Query: 181 GALVSASTGGYGCQGSTPLHLAARGGSLDCIRELLAWGADRLQRDSTGRIPYVIAMKHRH 240
           GALVSASTGGYGCQGSTPLHLAARGGSLDCIRELLAWGADRLQRDSTGRIPYVIAMKHRH
Sbjct: 181 GALVSASTGGYGCQGSTPLHLAARGGSLDCIRELLAWGADRLQRDSTGRIPYVIAMKHRH 240

Query: 241 GTCAALLNPSSTEPLVWPSPLKFINELNQEAKALLEQALMEANREREKNILKTNSPGLPS 300
           GTCAALLNPSSTEPLVWPSPLKFINELNQEAKALLEQALMEANREREKNILKTNSPGLPS
Sbjct: 241 GTCAALLNPSSTEPLVWPSPLKFINELNQEAKALLEQALMEANREREKNILKTNSPGLPS 300

Query: 301 PSHSDAVDDNLSEISDEDTELCSICFEQASTIEVQNCGHVMCAQCTLSLCCHSKPNPTTA 360
           PSHSDAVDDNLSEISDEDTELCSICFEQASTIEVQNCGHVMCAQCTLSLCCHSKPNPTTA
Sbjct: 301 PSHSDAVDDNLSEISDEDTELCSICFEQASTIEVQNCGHVMCAQCTLSLCCHSKPNPTTA 360

Query: 361 CLTPPVCPFCRSNILHLVVAKTKREEVDDHEAINSKQRKGRKSHNFSEGGSSSFKGLSSM 420
           CLTPPVCPFCRSNILHLVVAKTKREEVDDHEAINSKQRKGRKSHNFSEGGSSSFKGLSSM
Sbjct: 361 CLTPPVCPFCRSNILHLVVAKTKREEVDDHEAINSKQRKGRKSHNFSEGGSSSFKGLSSM 420

Query: 421 TSFGKIGGGRNSGRIAAEENQSVDKLG 447
           TSFGKIGGGRNSGRIAAEE+QSVDKLG
Sbjct: 421 TSFGKIGGGRNSGRIAAEEDQSVDKLG 447

BLAST of Cucsat.G5577 vs. ExPASy TrEMBL
Match: A0A6J1FYP2 (putative E3 ubiquitin-protein ligase XBAT31 OS=Cucurbita moschata OX=3662 GN=LOC111448919 PE=4 SV=1)

HSP 1 Score: 821 bits (2120), Expect = 4.73e-299
Identity = 410/448 (91.52%), Postives = 426/448 (95.09%), Query Frame = 0

Query: 1   MGQGLSCSATHENGLFSAVQCGDLEMVQLLLNNQPSLLHNTTLYDRQSPLHFAAANGQIE 60
           MGQGLSCSA+HENGLFSAVQCGDL MV++ L N+PSLL +TTLYDRQSPLH AAANGQIE
Sbjct: 1   MGQGLSCSASHENGLFSAVQCGDLAMVEVFLKNKPSLLLDTTLYDRQSPLHIAAANGQIE 60

Query: 61  ILSLLLERSVNPDLLNRHKQTPLMLAAMHGKISCLKKLLEVGANILKFDSLHGRTCLHYA 120
           ILSLLLER VNPDLLNRHKQTPLMLAAMHGKISCL+KLLEVGANILKFDSL+GRTCLHYA
Sbjct: 61  ILSLLLERCVNPDLLNRHKQTPLMLAAMHGKISCLRKLLEVGANILKFDSLNGRTCLHYA 120

Query: 121 AYYGHFDCLEVILSAAQSSPVAASWGFVRFVNIRDGKGATPLHLAARQRRPECIHILLDN 180
           AYYGHFDCLE ILSAAQSSPVAASWGFVRFVNIRDGKGATPLHLAAR+RRPECIHILLDN
Sbjct: 121 AYYGHFDCLEAILSAAQSSPVAASWGFVRFVNIRDGKGATPLHLAARRRRPECIHILLDN 180

Query: 181 GALVSASTGGYGCQGSTPLHLAARGGSLDCIRELLAWGADRLQRDSTGRIPYVIAMKHRH 240
           GALV ASTGGYGCQGSTPLHLAARGGSLDCIRELLAWGADRLQRDS+GRIPYVIAMKH+H
Sbjct: 181 GALVGASTGGYGCQGSTPLHLAARGGSLDCIRELLAWGADRLQRDSSGRIPYVIAMKHKH 240

Query: 241 GTCAALLNPSSTEPLVWPSPLKFINELNQEAKALLEQALMEANREREKNILKTNSPGLPS 300
           GTCAALLNPSSTEPLVWPSPLKFINELNQEAKALLEQALMEANREREK+ILKTNS    S
Sbjct: 241 GTCAALLNPSSTEPLVWPSPLKFINELNQEAKALLEQALMEANREREKSILKTNSHEFSS 300

Query: 301 PSHSDAVDDNLSEISDEDTELCSICFEQASTIEVQNCGHVMCAQCTLSLCCHSKPNPTTA 360
           PSHSDAVDD+LSEISD D ELCSICFEQ STIEVQNCGHVMCAQCTLSLCCHSKPNP TA
Sbjct: 301 PSHSDAVDDSLSEISDGDAELCSICFEQPSTIEVQNCGHVMCAQCTLSLCCHSKPNPATA 360

Query: 361 CLTPPVCPFCRSNILHLVVAKTK-REEVDDHEAINSKQRKGRKSHNFSEGGSSSFKGLSS 420
           CLTPPVCPFCRSNI+HLVV KTK +EEVDDHE  NSKQRKGRKSHNFSEGGSSSFKGLSS
Sbjct: 361 CLTPPVCPFCRSNIIHLVVKKTKTKEEVDDHETNNSKQRKGRKSHNFSEGGSSSFKGLSS 420

Query: 421 MTSFGKIGGGRNSGRIAAEENQSVDKLG 447
           MTSFGKI GGR+SGRIAAEE+Q VDKLG
Sbjct: 421 MTSFGKISGGRSSGRIAAEEDQFVDKLG 448

BLAST of Cucsat.G5577 vs. ExPASy TrEMBL
Match: A0A6J1JHA9 (putative E3 ubiquitin-protein ligase XBAT31 OS=Cucurbita maxima OX=3661 GN=LOC111484478 PE=4 SV=1)

HSP 1 Score: 820 bits (2119), Expect = 6.71e-299
Identity = 409/448 (91.29%), Postives = 425/448 (94.87%), Query Frame = 0

Query: 1   MGQGLSCSATHENGLFSAVQCGDLEMVQLLLNNQPSLLHNTTLYDRQSPLHFAAANGQIE 60
           MGQGLSCSA+HENGLFSAVQCGDL MV++ L N+PS+L NTTLYDRQSPLH AAANGQIE
Sbjct: 1   MGQGLSCSASHENGLFSAVQCGDLAMVEVFLKNKPSMLLNTTLYDRQSPLHIAAANGQIE 60

Query: 61  ILSLLLERSVNPDLLNRHKQTPLMLAAMHGKISCLKKLLEVGANILKFDSLHGRTCLHYA 120
           ILSLLLERSVNPDLLNRHKQTPLMLAAMHGKISCL+KLLEVGANILKFDSL+GRTCLHYA
Sbjct: 61  ILSLLLERSVNPDLLNRHKQTPLMLAAMHGKISCLRKLLEVGANILKFDSLNGRTCLHYA 120

Query: 121 AYYGHFDCLEVILSAAQSSPVAASWGFVRFVNIRDGKGATPLHLAARQRRPECIHILLDN 180
           AYYGHFDCLE ILSAAQSSPVAASWGFVRFVNIRDGKGATPLHLAARQRRPECIHILLDN
Sbjct: 121 AYYGHFDCLEAILSAAQSSPVAASWGFVRFVNIRDGKGATPLHLAARQRRPECIHILLDN 180

Query: 181 GALVSASTGGYGCQGSTPLHLAARGGSLDCIRELLAWGADRLQRDSTGRIPYVIAMKHRH 240
           GALV ASTGGYGCQGSTPLHLAARGGSLDCIRELLAWGADRLQRDS+GRIPYVIAMKH+H
Sbjct: 181 GALVGASTGGYGCQGSTPLHLAARGGSLDCIRELLAWGADRLQRDSSGRIPYVIAMKHKH 240

Query: 241 GTCAALLNPSSTEPLVWPSPLKFINELNQEAKALLEQALMEANREREKNILKTNSPGLPS 300
           GTCAALLNPSSTEPLVWPSPLKFINELNQEAKALLEQALMEANREREK+ILKTNS    S
Sbjct: 241 GTCAALLNPSSTEPLVWPSPLKFINELNQEAKALLEQALMEANREREKSILKTNSHEFSS 300

Query: 301 PSHSDAVDDNLSEISDEDTELCSICFEQASTIEVQNCGHVMCAQCTLSLCCHSKPNPTTA 360
           PSHSDAVDD+LSEISD D ELCSICFEQ STIEVQNCGHVMCAQCTLSLCCHSKPNP TA
Sbjct: 301 PSHSDAVDDSLSEISDGDAELCSICFEQPSTIEVQNCGHVMCAQCTLSLCCHSKPNPATA 360

Query: 361 CLTPPVCPFCRSNILHLVVAKTK-REEVDDHEAINSKQRKGRKSHNFSEGGSSSFKGLSS 420
           CLTPPVCPFCRSNI+HLVV KTK +EEVDDHE  NSKQRKGRKSHNF EGGSSSFKGLSS
Sbjct: 361 CLTPPVCPFCRSNIIHLVVKKTKTKEEVDDHETNNSKQRKGRKSHNFCEGGSSSFKGLSS 420

Query: 421 MTSFGKIGGGRNSGRIAAEENQSVDKLG 447
           MT FGKI GGR+SGRIAAEE++ VDKLG
Sbjct: 421 MTPFGKISGGRSSGRIAAEEDEFVDKLG 448

BLAST of Cucsat.G5577 vs. ExPASy TrEMBL
Match: A0A6J1D465 (putative E3 ubiquitin-protein ligase XBAT31 OS=Momordica charantia OX=3673 GN=LOC111017099 PE=4 SV=1)

HSP 1 Score: 807 bits (2085), Expect = 1.18e-293
Identity = 409/452 (90.49%), Postives = 426/452 (94.25%), Query Frame = 0

Query: 1   MGQGLSCSATHENGLFSAVQCGDLEMVQLLLNNQPSLLHNTTLYDRQSPLHFAAANGQIE 60
           MGQGLSCSATHENGLF AVQ GDLE V +LL N+PSLL NTT+YDRQS LH AAANGQIE
Sbjct: 1   MGQGLSCSATHENGLFRAVQNGDLEAVHVLLENEPSLLQNTTVYDRQSALHIAAANGQIE 60

Query: 61  ILSLLLERSVNPDLLNRHKQTPLMLAAMHGKISCLKKLLEVGANILKFDSLHGRTCLHYA 120
           ILSLLLERSVNPDLLNRHKQTPLMLAAMHGKISCL+KLLEVGANILKFDSLHGRTCLHYA
Sbjct: 61  ILSLLLERSVNPDLLNRHKQTPLMLAAMHGKISCLRKLLEVGANILKFDSLHGRTCLHYA 120

Query: 121 AYYGHFDCLEVILSAAQSSPVAASWGFVRFVNIRDGKGATPLHLAARQRRPECIHILLDN 180
           AYYGHFDC+E ILSAAQSSPVAASWGFVRFVNIRDG+GATPLHLAARQRRPECIHILLDN
Sbjct: 121 AYYGHFDCVEAILSAAQSSPVAASWGFVRFVNIRDGRGATPLHLAARQRRPECIHILLDN 180

Query: 181 GALVSASTGGYGCQGSTPLHLAARGGSLDCIRELLAWGADRLQRDSTGRIPYVIAMKHRH 240
           GALVSASTGGYGCQGSTPLHLAARGGSLDCIRELLAWGADRLQRDS+GRIPYVIAMKHRH
Sbjct: 181 GALVSASTGGYGCQGSTPLHLAARGGSLDCIRELLAWGADRLQRDSSGRIPYVIAMKHRH 240

Query: 241 GTCAALLNPSSTEPLVWPSPLKFINELNQEAKALLEQALMEANREREKNILKTNSPG-LP 300
           GTCAALLNPSSTEPLVWPSPLKFINELNQEAKALLEQALMEANREREKNILKT +   LP
Sbjct: 241 GTCAALLNPSSTEPLVWPSPLKFINELNQEAKALLEQALMEANREREKNILKTTTTNELP 300

Query: 301 SPSHSDAVDDNLSEISD-EDTELCSICFEQASTIEVQNCGHVMCAQCTLSLCCHSKPNPT 360
           SPSHSDAVD   SEIS  +D ELCSICFEQASTIEVQNCGHVMCAQCTLSLCC+SKP+PT
Sbjct: 301 SPSHSDAVDGYSSEISGGDDAELCSICFEQASTIEVQNCGHVMCAQCTLSLCCYSKPDPT 360

Query: 361 TACLTPPVCPFCRSNILHLVVAKTKR---EEVDDHEAINSKQRKGRKSHNFSEGGSSSFK 420
           TACLTPPVCPFCRSNI+ L VAKTK    EEVDDHEA NSKQRKGRKSHNFSEGGSSSFK
Sbjct: 361 TACLTPPVCPFCRSNIIRLAVAKTKTKTGEEVDDHEANNSKQRKGRKSHNFSEGGSSSFK 420

Query: 421 GLSSMTSFGKIGGGRNSGRIAAEENQSVDKLG 447
           GLS+M+SFGKIGGGR+SGRIAAE++QSVDKLG
Sbjct: 421 GLSAMSSFGKIGGGRSSGRIAAEDDQSVDKLG 452

BLAST of Cucsat.G5577 vs. TAIR 10
Match: AT2G28840.1 (XB3 ortholog 1 in Arabidopsis thaliana )

HSP 1 Score: 609.4 bits (1570), Expect = 2.4e-174
Identity = 316/459 (68.85%), Postives = 371/459 (80.83%), Query Frame = 0

Query: 1   MGQGLSCSATHENGLFSAVQCGDLEMVQLLLNNQPSLLHNTTLYDRQSPLHFAAANGQIE 60
           MGQ +SC +  E+G+F++VQCGD+  ++ ++  +PSLL+ TT YDR S LH AAANGQIE
Sbjct: 1   MGQSMSCGSRPEHGIFASVQCGDIITIRRVMATEPSLLNQTTPYDRHSVLHVAAANGQIE 60

Query: 61  ILSLLLERSVNPDLLNRHKQTPLMLAAMHGKISCLKKLLEVGANILKFDSLHGRTCLHYA 120
           ILSLLLER  NPDLLNRHKQTPLMLAAM+G+ISC+KKL EVGANIL FDS++ RTCLHYA
Sbjct: 61  ILSLLLERFTNPDLLNRHKQTPLMLAAMYGRISCVKKLAEVGANILMFDSVNRRTCLHYA 120

Query: 121 AYYGHFDCLEVILSAAQSSPVAASWGFVRFVNIRDGKGATPLHLAARQRRPECIHILLDN 180
           AYYGH +C++ ILSAAQSSPVA  WG+ RFVNIRD KGATPLHLAARQRRPEC+++LLD+
Sbjct: 121 AYYGHANCVQAILSAAQSSPVAVHWGYARFVNIRDDKGATPLHLAARQRRPECVNVLLDS 180

Query: 181 GALVSASTGGYGCQGSTPLHLAARGGSLDCIRELLAWGADRLQRDSTGRIPYVIAMKHRH 240
           G+LV AST  YG  GSTPLHLAAR GS+DC+R+LLAWGADRLQRD++GRIPYV+AMKH+H
Sbjct: 181 GSLVCASTSVYGSPGSTPLHLAARSGSIDCVRKLLAWGADRLQRDASGRIPYVVAMKHKH 240

Query: 241 GTCAALLNPSSTEPLVWPSPLKFINELNQEAKALLEQALMEANREREKNILKTNSPGLPS 300
           G C ALLNPSS EPLVWPSPLKFI+ELN EAK LLEQALMEANREREK ILK  +  LPS
Sbjct: 241 GACGALLNPSSAEPLVWPSPLKFISELNDEAKLLLEQALMEANREREKTILKGTAYSLPS 300

Query: 301 PSHSDAVDDNLSEISDEDTELCSICFEQASTIEVQNCGHVMCAQCTLSLCCHSKPNPTTA 360
           PS SD  DDN+SE+S  DTELC ICFEQ  TIEV++CGH MCAQCTL+LCCH+KPNPTT+
Sbjct: 301 PSFSD-TDDNMSEVS--DTELCCICFEQVCTIEVKDCGHQMCAQCTLALCCHNKPNPTTS 360

Query: 361 CLTPPVCPFCRSNILHLVVAKT------KREEVDD--------HEAINSKQRKGRKSHNF 420
            +TPPVCPFCRS I  LVVA+       K + +DD         +  +SK RK R+S N 
Sbjct: 361 TVTPPVCPFCRSTIACLVVAQNNNNNNEKSKSLDDVVVVDREAGDVSSSKFRKHRRSINL 420

Query: 421 SEGGSSSFKGLSSMTSFGKIGGGRNSGRIAAEENQSVDK 446
            E  SSSF GLS++ SFG+I  GR SGRIAA EN+ +DK
Sbjct: 421 GE-ESSSFMGLSTIGSFGRI-TGRGSGRIAA-ENELMDK 453

BLAST of Cucsat.G5577 vs. TAIR 10
Match: AT2G28840.2 (XB3 ortholog 1 in Arabidopsis thaliana )

HSP 1 Score: 540.8 bits (1392), Expect = 1.0e-153
Identity = 283/401 (70.57%), Postives = 327/401 (81.55%), Query Frame = 0

Query: 59  IEILSLLLERSVNPDLLNRHKQTPLMLAAMHGKISCLKKLLEVGANILKFDSLHGRTCLH 118
           ++ILSLLLER  NPDLLNRHKQTPLMLAAM+G+ISC+KKL EVGANIL FDS++ RTCLH
Sbjct: 45  LQILSLLLERFTNPDLLNRHKQTPLMLAAMYGRISCVKKLAEVGANILMFDSVNRRTCLH 104

Query: 119 YAAYYGHFDCLEVILSAAQSSPVAASWGFVRFVNIRDGKGATPLHLAARQRRPECIHILL 178
           YAAYYGH +C++ ILSAAQSSPVA  WG+ RFVNIRD KGATPLHLAARQRRPEC+++LL
Sbjct: 105 YAAYYGHANCVQAILSAAQSSPVAVHWGYARFVNIRDDKGATPLHLAARQRRPECVNVLL 164

Query: 179 DNGALVSASTGGYGCQGSTPLHLAARGGSLDCIRELLAWGADRLQRDSTGRIPYVIAMKH 238
           D+G+LV AST  YG  GSTPLHLAAR GS+DC+R+LLAWGADRLQRD++GRIPYV+AMKH
Sbjct: 165 DSGSLVCASTSVYGSPGSTPLHLAARSGSIDCVRKLLAWGADRLQRDASGRIPYVVAMKH 224

Query: 239 RHGTCAALLNPSSTEPLVWPSPLKFINELNQEAKALLEQALMEANREREKNILKTNSPGL 298
           +HG C ALLNPSS EPLVWPSPLKFI+ELN EAK LLEQALMEANREREK ILK  +  L
Sbjct: 225 KHGACGALLNPSSAEPLVWPSPLKFISELNDEAKLLLEQALMEANREREKTILKGTAYSL 284

Query: 299 PSPSHSDAVDDNLSEISDEDTELCSICFEQASTIEVQNCGHVMCAQCTLSLCCHSKPNPT 358
           PSPS SD  DDN+SE+S  DTELC ICFEQ  TIEV++CGH MCAQCTL+LCCH+KPNPT
Sbjct: 285 PSPSFSD-TDDNMSEVS--DTELCCICFEQVCTIEVKDCGHQMCAQCTLALCCHNKPNPT 344

Query: 359 TACLTPPVCPFCRSNILHLVVAKT------KREEVDD--------HEAINSKQRKGRKSH 418
           T+ +TPPVCPFCRS I  LVVA+       K + +DD         +  +SK RK R+S 
Sbjct: 345 TSTVTPPVCPFCRSTIACLVVAQNNNNNNEKSKSLDDVVVVDREAGDVSSSKFRKHRRSI 404

Query: 419 NFSEGGSSSFKGLSSMTSFGKIGGGRNSGRIAAEENQSVDK 446
           N  E  SSSF GLS++ SFG+I  GR SGRIAA EN+ +DK
Sbjct: 405 NLGE-ESSSFMGLSTIGSFGRI-TGRGSGRIAA-ENELMDK 439

BLAST of Cucsat.G5577 vs. TAIR 10
Match: AT5G07270.1 (XB3 ortholog 3 in Arabidopsis thaliana )

HSP 1 Score: 169.5 bits (428), Expect = 6.2e-42
Identity = 136/398 (34.17%), Postives = 185/398 (46.48%), Query Frame = 0

Query: 1   MGQGLSCSATHENGLFSAVQCGDLEMVQLLLNNQPSLLHNTTLYDRQSPLHFAAANGQIE 60
           MG    CSA+ E  L SA + GD    ++LL+  P L   +T     SPLHFAAA G  E
Sbjct: 1   MGNSFGCSASGER-LVSAARDGDFVEAKMLLDCNPCLAKYSTFGGLNSPLHFAAAKGHNE 60

Query: 61  ILSLLLERSVNPDLLNRHKQTPLMLAAMHGKISCLKKLLEVGANILKFDSLHGRTCLHYA 120
           I+ LLLE   + +  N   QT LM A  +G    ++ LL    N+ + D L GRT LH+A
Sbjct: 61  IVGLLLENGADVNSRNYCGQTALMQACRYGHWEVVQTLLLFRCNVTRADYLAGRTALHFA 120

Query: 121 AYYGHFDCLEVIL----------SAAQSSPVAA-----SWGFVRFVNIRDGKGATPLHLA 180
           A  GH  C+ ++L          S  ++  V A          +FVN     G T LH+A
Sbjct: 121 AVNGHARCIRLVLADFLPSDKLNSLPETGVVTAKNKSEQSALSKFVNKAADGGITALHMA 180

Query: 181 ARQRRPECIHILLDNGALVSASTGGYGCQ------GSTPLHLAARGGSLDCIRELLAWGA 240
           A     +C+ +LLD  A VSA T  YG        GSTPLH AA GG+L C + LLA GA
Sbjct: 181 ALNGLFDCVQLLLDLEANVSAVTFHYGTSMDMIGAGSTPLHYAACGGNLKCCQILLARGA 240

Query: 241 DRLQRDSTGRIPYVIAMKHRHGTCAALLNPSSTEPLVWPSPLKFINELNQEAKALLEQAL 300
            ++  +  G +P  IA          LL+P+S   +V P+     N L+    ++L    
Sbjct: 241 RKMTLNCNGWLPIDIARMWSRHWLEPLLSPNS--DVVIPA-FPHSNYLSLPLLSIL---- 300

Query: 301 MEANREREKNILKTNSPGLPSPSHSDAVDDNLSEISDEDTELCSICFEQASTIEVQNCGH 360
              N  RE         GL S +  D VD            +C++C E+  T+  + C H
Sbjct: 301 ---NIAREF--------GLQSATIGDEVD------------ICAVCLERTCTVAAEGCEH 360

Query: 361 VMCAQCTLSLCCHSKPNPTTACLTPP---VCPFCRSNI 375
            +C +C L LC  S  N  +  + PP    CP CR  I
Sbjct: 361 QLCVRCALYLC--SSSNVPSVTVGPPGSIPCPLCRHGI 365

BLAST of Cucsat.G5577 vs. TAIR 10
Match: AT5G57740.1 (XB3 ortholog 2 in Arabidopsis thaliana )

HSP 1 Score: 155.6 bits (392), Expect = 9.3e-38
Identity = 127/403 (31.51%), Postives = 181/403 (44.91%), Query Frame = 0

Query: 1   MGQGLSCSATHENGLFSAVQCGDLEMVQLLLNNQPSLLHNTTLYDRQSPLHFAAANGQIE 60
           +G    CSA+ E  L SA + GDL+  + LL+  P L   +T   R SPLH++AA G  E
Sbjct: 7   VGNSFGCSASGER-LVSAARDGDLQEAKALLDYNPRLARYSTFGVRNSPLHYSAAQGHHE 66

Query: 61  ILSLLLERSVNPDLLNRHKQTPLMLAAMHGKISCLKKLLEVGANILKFDSLHGRTCLHYA 120
           I+SLL+E  V+ +L N   QT LM A  HG    +  L+  GANI + D L+G T LH A
Sbjct: 67  IVSLLVESGVDINLRNYRGQTALMQACQHGHWEVVLILILFGANIHRSDYLNGGTALHLA 126

Query: 121 AYYGHFDCLEVILSAAQSSPVAASWGFVR----------------FVNIRDGKGATPLHL 180
           A  GH  C+ ++LS    S V   W  ++                 +N     G TPLH+
Sbjct: 127 ALNGHPRCIRILLSEYIPS-VPNCWSLLKNKKTSVAGFDSSVLHEVINRAADGGITPLHV 186

Query: 181 AARQRRPECIHILLDNGALVSASTGGYGC------QGSTPLHLAARGGSLDCIRELLAWG 240
           AA     E + +LLD GA V+  T   G        GST LH A+ GG+  C + L++ G
Sbjct: 187 AALNGHIETVQLLLDLGASVTQVTVEDGTTIDLIGAGSTALHYASCGGNTQCCQLLISKG 246

Query: 241 ADRLQRDSTGRIPYVIAMKHRHGTCAALLNPSSTEPLVW----PSPLKFINELNQEAKAL 300
           A     +S G  P ++A          +LNP++ +P +     PSP      L     ++
Sbjct: 247 ACLAAVNSNGWTPMMVARSWHRNWLEEILNPTTEQPQLHLPNVPSPF-----LCLPLMSI 306

Query: 301 LEQALMEANREREKNILKTNSPGLPSPSHSDAVDDNLSEISDEDTELCSICFEQASTIEV 360
           +  A     RE                      +D L+   D     C++C E+  T+  
Sbjct: 307 VNIAQECGWRE----------------------NDCLTPCRDP----CAVCLERKCTVAA 366

Query: 361 QNCGHVMCAQCTLSLCCHSKPNPTTACLTP--PVCPFCRSNIL 376
             C H  C  C L L   S  +  T+ +TP    CP CR+ I+
Sbjct: 367 DGCAHEFCTNCALYLSTTSITSSKTSNVTPGSVPCPLCRNGIV 376

BLAST of Cucsat.G5577 vs. TAIR 10
Match: AT3G04710.1 (ankyrin repeat family protein )

HSP 1 Score: 95.1 bits (235), Expect = 1.5e-19
Identity = 85/280 (30.36%), Postives = 127/280 (45.36%), Query Frame = 0

Query: 45  DRQSPLHFAAANGQIEILSLLLER-SVNPDLLNRHKQTPLMLAAMHGKISCLKKLLEVGA 104
           +++  LHFAA  GQ EI   LLE   +N D  +    TPL+ AA  G+I  +K LLE GA
Sbjct: 54  NKRGALHFAAREGQTEICRYLLEELKLNADAKDETGDTPLVHAARQGQIETVKYLLEQGA 113

Query: 105 NILKFDSLHGRTCLHYAAYYGHFDCLEVILSAAQSSPVAASWGFVRFVNIRDGKGATPLH 164
           +     S  G T LH+AA  G  + L+ +LS  +  PV +           + +  TPL 
Sbjct: 114 D-PNIASELGATALHHAAGTGEIELLKELLS--RGVPVDS-----------ESESGTPLI 173

Query: 165 LAARQRRPECIHILLDNGALVSAST-------------GGYGC---------------QG 224
            AA   +   + +LL++ A  +A T             G   C                G
Sbjct: 174 WAAGHDQKNAVEVLLEHNANPNAETEDNITPLLSAVAAGSLSCLELLVKAGAKANVFAGG 233

Query: 225 STPLHLAARGGSLDCIRELLAWGADRLQRDSTGRIPYVIAMKHRHGTCAALLNPSSTEPL 284
           +TPLH+AA  G+L+ I  LL  GAD  Q+D  G  P  +A    +     +L P +T+P 
Sbjct: 234 ATPLHIAADIGNLELINCLLKAGADPNQKDEEGNRPLEVAAARDNRKVVEILFPLTTKP- 293

Query: 285 VWPSPLKFINELNQEAKALLEQALMEANREREKNILKTNS 296
                 + +++   +       A ME+N+E+E+N  K  S
Sbjct: 294 ------ETVSDWTVDGIL----AHMESNKEQEENSNKAKS 308

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94B553.3e-17368.85Putative E3 ubiquitin-protein ligase XBAT31 OS=Arabidopsis thaliana OX=3702 GN=X... [more]
Q65XV22.6e-15764.22E3 ubiquitin-protein ligase XB3 OS=Oryza sativa subsp. japonica OX=39947 GN=XB3 ... [more]
Q94CT77.0e-13156.43Probable E3 ubiquitin-protein ligase XBOS31 OS=Oryza sativa subsp. japonica OX=3... [more]
Q337A07.9e-4231.23Probable E3 ubiquitin-protein ligase XBOS33 OS=Oryza sativa subsp. japonica OX=3... [more]
Q4FE458.8e-4134.17E3 ubiquitin-protein ligase XBAT33 OS=Arabidopsis thaliana OX=3702 GN=XBAT33 PE=... [more]
Match NameE-valueIdentityDescription
XP_004143108.10.0100.00putative E3 ubiquitin-protein ligase XBAT31 [Cucumis sativus] >KGN47159.1 hypoth... [more]
XP_008464140.10.098.66PREDICTED: putative E3 ubiquitin-protein ligase XBAT31 [Cucumis melo][more]
XP_038902234.10.096.65putative E3 ubiquitin-protein ligase XBAT31 [Benincasa hispida][more]
KAG6570711.18.38e-30091.74putative E3 ubiquitin-protein ligase XBAT31, partial [Cucurbita argyrosperma sub... [more]
XP_023511973.13.41e-29991.52putative E3 ubiquitin-protein ligase XBAT31 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A0A0KE960.0100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G190990 PE=4 SV=1[more]
A0A1S3CKT30.098.66putative E3 ubiquitin-protein ligase XBAT31 OS=Cucumis melo OX=3656 GN=LOC103502... [more]
A0A6J1FYP24.73e-29991.52putative E3 ubiquitin-protein ligase XBAT31 OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1JHA96.71e-29991.29putative E3 ubiquitin-protein ligase XBAT31 OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1D4651.18e-29390.49putative E3 ubiquitin-protein ligase XBAT31 OS=Momordica charantia OX=3673 GN=LO... [more]
Match NameE-valueIdentityDescription
AT2G28840.12.4e-17468.85XB3 ortholog 1 in Arabidopsis thaliana [more]
AT2G28840.21.0e-15370.57XB3 ortholog 1 in Arabidopsis thaliana [more]
AT5G07270.16.2e-4234.17XB3 ortholog 3 in Arabidopsis thaliana [more]
AT5G57740.19.3e-3831.51XB3 ortholog 2 in Arabidopsis thaliana [more]
AT3G04710.11.5e-1930.36ankyrin repeat family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 72..99
NoneNo IPR availablePFAMPF13857Ank_5coord: 6..46
e-value: 1.1E-7
score: 31.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 217..237
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 196..216
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 196..258
NoneNo IPR availablePANTHERPTHR24128HOMEOBOX PROTEIN WARIAIcoord: 4..249
NoneNo IPR availablePANTHERPTHR24128:SF57UBIQUITIN-PROTEIN LIGASE XBAT31, PUTATIVE-RELATEDcoord: 4..249
NoneNo IPR availablePROSITEPS50297ANK_REP_REGIONcoord: 5..31
score: 12.03652
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 124..187
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 117..193
e-value: 1.4E-8
score: 35.8
IPR036770Ankyrin repeat-containing domain superfamilyGENE3D1.25.40.20coord: 2..64
e-value: 3.3E-12
score: 48.4
IPR036770Ankyrin repeat-containing domain superfamilySUPERFAMILY48403Ankyrin repeatcoord: 5..59
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 133..182
score: 9.937009
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 5..37
score: 13.09007

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G5577.T1Cucsat.G5577.T1mRNA
Cucsat.G5577.T3Cucsat.G5577.T3mRNA
Cucsat.G5577.T2Cucsat.G5577.T2mRNA
Cucsat.G5577.T4Cucsat.G5577.T4mRNA
Cucsat.G5577.T5Cucsat.G5577.T5mRNA
Cucsat.G5577.T6Cucsat.G5577.T6mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006464 cellular protein modification process
biological_process GO:0016310 phosphorylation
biological_process GO:0009408 response to heat
molecular_function GO:0140096 catalytic activity, acting on a protein
molecular_function GO:0016301 kinase activity
molecular_function GO:0005515 protein binding
molecular_function GO:0016740 transferase activity