Cucsat.G3859 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G3859
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Pectinesterase
Location	ctg105: 3688106 .. 3690953 (+)
RNA-Seq Expression	Cucsat.G3859
Synteny	Cucsat.G3859

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATAATAATATGGCCATGGGTCCTGGAGATATGACAACAGCAAGGCTAATAAGAGTGGATGGATCAGGAAATGGAGACTTCAAAAAAATTCAACAAGCAATTGATTCTGTTCCTTCTCAAAACAATGAACTCGTTTTCATTTGGGTTAAGCCTGGAACTTACAGGTAATTATTAATTGTTCCTTCATTTTCTTATTTTCATTTTGGTCACTCTTCTTCTTCTTCTTCTTCTTCTTTTCTCTCAATTTCTTTGATCGGGTGCTTGTAACACGATTATATGATTTGAACTCTTTAAGATTAGATCGAAGGAGAGAATTAGTTGAAATAAAGCATAGTTCAACCGGCATATTTAAACTTGTTATATGAGTTTAAGGTTAAGAAAATTGAGTTTTTAGTTTTCAAACTTGTGGTTGTTTTAACTTTTGAAGTTTTGAATTTGTTAACATTCTTCGTTCTTTATGTTTTAGGGTTCATTTTTTTATTATGGTCAACTTTTTTGTTATATTTATTGTTGTGTTGCGGTTGTGTCATGTTCGCATGGCCTCTTTCTGTTCATTTCTTTAAGTTTATATATACATGAAAAAAAAAAAAAGCAGAGAGAAGGAGAAGATAACAAAAGAAAAGAAATAGATGCGAGGATGAAGAAGGATCATAGAATTTTAAATAGTTCTATATTAGAAAAATATTGTGATTGAATAGGGTTTGATAGATTCTAAAATTTGGTTAAATTTTATATCGTTAGAAAGAATTTTATTTTTAAAAATTATATGGATAATTTTAATTAAAAACAATGACAACATATATACTTTTGTTTATGTGGTGTGTTGATAACAAATATTGAGTAGAGAGAAAATAGTGGTACCTGAGGACAAGCCCTACATAACAATAAGCGGCAGTAAGGCCTCCGATACAAAAATCACATGGAACCAAGGAAGAGATTTACTTGAATCTCCGGTTGTATCTATCTTTGCTTCTGATTTTGTCGGTCGTTTTCTGACAATCGAAAACACATTTGGCACAACTGGCATTGCGGTGGCATTAAGGGTTTCCGCCGATAGAGCAGCCTTTTACGGCTGTCGGATCATCTCCTTTCAAGACACCCTTTTGGACGACACTGGAAGACATTACTTCAATAATTGTTACATAGAAGGAGCCACCGATTTCATTTGCGGGAATGCCGCTTCTCTATACGAGGTTTTTTTTTCCATCTCTTGATATATTCATTACATACTAGCTAGTTAAGTTATATTTAAGGAGTATATATGGAAGGTTTCTTATATGTGGATTGTACTATAGAAGTGTCATTTGCATTCGACGTCGGATAGAGGTGGGGCGATGACGGCGCAGCATCGAAACACGGGGGAGGAAAACACAGGGTTCGTGTTCTTGGGAGGAAAAATAACAGGAAGTGGATCCATGTTTCTTGGACGGCCATGGGGGGATTTCTCTAAAGTTGTGTTTGGGTACACTTACATGTCCAATGTTGTTGAACCTGAGGGTTGGAATGATTGGGGAGATCCAACCAAGCAAAGGTAACATTATTATTTCTTTATAAGCTGTGATTTATAGCATATTTAAAATTACTTTTGTTATTTCTTTTTAAATCATTATAAAACATGTTTGTAATCCTTCAAGCTAATTTTAATTTTGTATAGATCAAAGTTGCATTTATGAAGTGTAAATTTCAAAAATTATTTTAAAAGAAAAGTGATTATGATGATTTTAAAATCACTACTCTTTCACTTAGTTCATTTGAATGGTTTTTGAAAATTTTAATTTAGTCTTAGTTACTTCGATATATAAGAATTTCCACAAAAACAAGGTGAAAAATGGTTTAAAAAATATATTTTTTATTTTCAATTTCAATGTTATCGTGTGAAAAGTTTTCATAATTTAGAAATATATATTACAAAGTCACAGTAAAGAAACTCTAATATACTATATGAAAAATTATTAAATTAAGAATCTCTACTATATACTAAATGAATTAAGTGAAAAATTATTAAATTTAAGAATTTAACTGATGTCTTAGGTGTTAACTTTTAGTTTATATAATTTTTAAAAGTTTTAATTTAGTCTTTGTTATTTGAATTTCCTTTCTGTAGTTTTACAAGTTAAAATAATTGCAAATATATATATACATATATATGAATAAAAAATTAAAAGAAAAACTACAAAAAAATCTCTCTGAAACGACGAAAAGAAACTTCGAAATAATTTCTTTCTCGCAAATTTATAACATTCCAACGCATAAATAAATTTTCTAGTTCAGATGATGTGAAAGCAAAACCACGTATAGGGTTCTTTTATATAATGCTCGATTTGCTGGTCACTTTATTTTTTATATATATTTAAATGCAGGTTTGCTGTTTGTTTGTTGTTATATTTTACCAAAAACTCTACAAATATAATTAAGATTGGTAAAACTAAATGTAATTTATAAATTGAGATTTTTGTGAAATCATGCACATGTATACAGAACGGTGTTGTATGGAGAATACAAATGTTATGGGCTCGGAGCAAACAGAGATAAAAGAGTGGTATGGTCACGTAGTTTGTCGACGGATGAGGCTTCAAAACTTTTCACCAAAGACATCATTGGTGGTCGAGCTTGGCTTCGACCTGCACCATCTCATTTCAAAGGGGGCTTTCCCTAGCTAGCACTAGCTCCATCATCACTTGCCACAACCAATATTTTCTTAGTTACATTTTGATTCTTTGGTTGTAATTTCATGCTATCAGCTCTCTAATTTCTTTTAGCTAATTTTATATCTCTTGTTTTCACGGTATTACATTTTCATTGTTAAGTCAACATTCCCATTATATTTTTAAAAATTCATGAAGTTATAAAT

Coding sequence (CDS)

ATGACGGCGCAGCATCGAAACACGGGGGAGGAAAACACAGGGTTCGTGTTCTTGGGAGGAAAAATAACAGGAAGTGGATCCATGTTTCTTGGACGGCCATGGGGGGATTTCTCTAAAGTTGTGTTTGGGTACACTTACATGTCCAATGTTGTTGAACCTGAGGGTTGGAATGATTGGGGAGATCCAACCAAGCAAAGAACGGTGTTGTATGGAGAATACAAATGTTATGGGCTCGGAGCAAACAGAGATAAAAGAGTGGTATGGTCACGTAGTTTGTCGACGGATGAGGCTTCAAAACTTTTCACCAAAGACATCATTGGTGGTCGAGCTTGGCTTCGACCTGCACCATCTCATTTCAAAGGGGGCTTTCCCTAG

Protein sequence

MTAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDWGDPTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLRPAPSHFKGGFP

Homology

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BLAST of Cucsat.G3859 vs. ExPASy Swiss-Prot
Match: Q9SIJ9 (Putative pectinesterase 11 OS=Arabidopsis thaliana OX=3702 GN=PME11 PE=3 SV=1)

HSP 1 Score: 434.5 bits (1116), Expect = 1.0e-120
Identity = 198/297 (66.67%), Postives = 242/297 (81.48%), Query Frame = 0


Query: 9   DMTTARLIRVDGSGNGDFKKIQQAIDSVPS--QNNELVFIWVKPGTYREKIVVPEDKPYI 68
           D +TA LIRVD SG GDF KIQ+AI+S+P    N++L FIWVKPG YREK+V+P +KPYI
Sbjct: 44  DFSTAILIRVDQSGKGDFSKIQEAIESIPPNLNNSQLYFIWVKPGIYREKVVIPAEKPYI 103

Query: 69  TISGSKASDTKITWNQGRDLLESPVVSIFASDFVGRFLTIENTFGTTGIAVALRVSADRA 128
           T+SG++AS+T + W+ G D+LESP ++IFASDFV RFLTI+N FGT G AVALRV+AD+A
Sbjct: 104 TLSGTQASNTFLIWSDGEDILESPTLTIFASDFVCRFLTIQNKFGTAGRAVALRVAADKA 163

Query: 129 AFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHSTSDRGGAMTA 188
           AFYGC I S+QDTLLDD G HYF NCYIEGATDFICG+A+SLYE+CHLHS S   G++TA
Sbjct: 164 AFYGCVITSYQDTLLDDNGNHYFKNCYIEGATDFICGSASSLYERCHLHSLSPNNGSITA 223

Query: 189 QHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDWGDPT 248
           Q R +  E +GF FLG K+TGSGS FLGRPWG +S+VVF Y++ SNVV P+GWN WGD T
Sbjct: 224 QMRTSATEKSGFTFLGCKLTGSGSTFLGRPWGAYSRVVFAYSFFSNVVAPQGWNQWGDST 283

Query: 249 KQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLRPAPSHFK 304
           K+ TV YGEYKCYG GA+R++RV WS+ LS +EA+   +KD IGG+ WLRPAPSHFK
Sbjct: 284 KENTVYYGEYKCYGPGADREQRVEWSKQLSDEEATVFLSKDFIGGKDWLRPAPSHFK 340

[Close]

BLAST of Cucsat.G3859 vs. ExPASy Swiss-Prot
Match: Q8VYZ3 (Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 2.1e-73
Identity = 140/292 (47.95%), Postives = 186/292 (63.70%), Query Frame = 0


Query: 21  SGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPEDKPYITISGSKASDTKITW 80
           S  GDF KIQ AIDS+P  N   V I V  G Y+EK+ +P  K +ITI G  A  T + W
Sbjct: 91  SNKGDFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTVEW 150

Query: 81  N---QGRDLLESPV-------VSIFASDFVGRFLTIENTF-----GTTG-IAVALRVSAD 140
               Q  D   +P+        ++ +  FV + +T  NT      G  G  AVALRVSAD
Sbjct: 151 GDTAQTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSAD 210

Query: 141 RAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHSTSDRGGAM 200
            AAF+GCR++  QDTL D  GRHY+ +CYIEG+ DFI GNA SLYE CH+H+ +D+ GA+
Sbjct: 211 NAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKLGAV 270

Query: 201 TAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDWGD 260
           TAQ R++  E+TGF F+  K+TG+G ++LGR WG FS+VVF YTYM N++ P GW +WGD
Sbjct: 271 TAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWYNWGD 330

Query: 261 PTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLR 297
           P+++ TV YG+YKC G GAN   RV W+R L+ +EA    +   I G  W++
Sbjct: 331 PSREMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFLSLTFIDGSEWIK 382

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BLAST of Cucsat.G3859 vs. ExPASy Swiss-Prot
Match: Q9FM79 (Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=2 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 6.2e-70
Identity = 133/300 (44.33%), Postives = 188/300 (62.67%), Query Frame = 0


Query: 14  RLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPEDKPYITISG--S 73
           R+I VD +G GD   +Q A+D VP  N++ V I++ PG YREK++VP+ KPYI+  G  S
Sbjct: 80  RVIVVDKNGGGDSVTVQGAVDMVPDSNSQRVKIFILPGIYREKVIVPKSKPYISFIGNES 139

Query: 74  KASDTKITWN--------QGRDL--LESPVVSIFASDFVGRFLTIENTF----GTTG-IA 133
            A DT I+W+         G++L    +  VSI +  F    +T ENT     G  G  A
Sbjct: 140 YAGDTVISWSDKASDLGCDGKELGTYRTASVSIESDFFCATAITFENTVVAEAGEQGRQA 199

Query: 134 VALRVSADRAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHS 193
           VALR+  D+A FY  R++  QDTL DD G HYF  CYI+G  DFI GNA SLY+ C +HS
Sbjct: 200 VALRIIGDKAVFYRVRVLGSQDTLFDDNGSHYFYQCYIQGNVDFIFGNAKSLYQDCDIHS 259

Query: 194 TSDRGGAMTAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEP 253
           T+ R GA+ A HR++  E+TGF F+   I+G+G ++LGR WG++S+ V+   ++++++ P
Sbjct: 260 TAKRYGAIAAHHRDSETEDTGFSFVNCDISGTGQIYLGRAWGNYSRTVYSNCFIADIITP 319

Query: 254 EGWNDWGDPTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLR 297
            GW+DW  P +QR V++GEY C G GA R  RV WS++L+ DE      ++ I G  WLR
Sbjct: 320 VGWSDWKHPERQRKVMFGEYNCRGRGAERGGRVPWSKTLTRDEVKPFLGREFIYGDQWLR 379

[Close]

BLAST of Cucsat.G3859 vs. ExPASy Swiss-Prot
Match: Q9ZQA3 (Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 1.6e-65
Identity = 128/300 (42.67%), Postives = 179/300 (59.67%), Query Frame = 0


Query: 12  TARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPEDKPYITISGS 71
           T+ ++ VD  G G+F  +Q AID VP  ++    I V  G+YREK+ V E+K  + I G 
Sbjct: 87  TSLVLTVDLHGCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENKTNLVIQGR 146

Query: 72  KASDTKITWN----QGRDLLESPVVSIFASDFVGRFLTIENTF------GTTGIAVALRV 131
              +T I WN       +  +S    +FA++F    ++ +N             AVALR+
Sbjct: 147 GYQNTSIEWNDTAKSAGNTADSFSFVVFAANFTAYNISFKNNAPEPDPGEADAQAVALRI 206

Query: 132 SADRAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHS----- 191
             D+AAFYGC     QDTLLDD GRH+F  C+I+G+ DFI GN  SLY+ C ++S     
Sbjct: 207 EGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRSLYQDCTINSIAKGN 266

Query: 192 TSDRGGAMTAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEP 251
           TS   G++TAQ R + +E +GF F+  KI GSG + LGR WG ++ VVF  TYMS ++ P
Sbjct: 267 TSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGRAWGAYATVVFSNTYMSGIITP 326

Query: 252 EGWNDWGDPTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLR 297
           EGWN+WGD TK++TV +GE+KCYG GA+  +RV++ + L+  EAS       I G  WLR
Sbjct: 327 EGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFGKQLTDSEASSFIDVSFIDGDEWLR 386

[Close]

BLAST of Cucsat.G3859 vs. ExPASy Swiss-Prot
Match: Q8LPF3 (Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 2.7e-65
Identity = 123/301 (40.86%), Postives = 176/301 (58.47%), Query Frame = 0


Query: 14  RLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPEDKPYITISGSKA 73
           ++I V  +G+  F+ +Q A+DS+P  NN+ + I + PG YREK+VVP  KPYIT  G+  
Sbjct: 59  KVITVSLNGHAQFRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGR 118

Query: 74  SDTKITWN--------QGRDL--LESPVVSIFASDFVGRFLTIENTF-----GTTG-IAV 133
             T I W+         G+ L   ++  V+++A+ F  R ++  NT      G  G  AV
Sbjct: 119 DVTAIEWHDRASDLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQAV 178

Query: 134 ALRVSADRAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHST 193
           A R+S D+A F GC     QDTL DD GRHYF  CYIEG+ DFI GN  S+Y+ C LHS 
Sbjct: 179 AFRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSI 238

Query: 194 SDRGGAMTAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPE 253
           + R G++ A  R   EE TGF F+G ++TG+G +++GR  G +S++V+ YTY   +V   
Sbjct: 239 ASRFGSIAAHGRTCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYFDALVAHG 298

Query: 254 GWNDWGDPT-KQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLR 298
           GW+DW   + K +T  +G Y CYG GA   + V W+R+L  + A     K  + GR W+ 
Sbjct: 299 GWDDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSFVNGRHWIA 358

[Close]

BLAST of Cucsat.G3859 vs. NCBI nr
Match: XP_004148862.1 (putative pectinesterase 11 [Cucumis sativus])

HSP 1 Score: 640 bits (1651), Expect = 6.76e-232
Identity = 307/307 (100.00%), Postives = 307/307 (100.00%), Query Frame = 0


Query: 1   NNMAMGPGDMTTARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVP 60
           NNMAMGPGDMTTARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVP
Sbjct: 8   NNMAMGPGDMTTARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVP 67

Query: 61  EDKPYITISGSKASDTKITWNQGRDLLESPVVSIFASDFVGRFLTIENTFGTTGIAVALR 120
           EDKPYITISGSKASDTKITWNQGRDLLESPVVSIFASDFVGRFLTIENTFGTTGIAVALR
Sbjct: 68  EDKPYITISGSKASDTKITWNQGRDLLESPVVSIFASDFVGRFLTIENTFGTTGIAVALR 127

Query: 121 VSADRAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHSTSDR 180
           VSADRAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHSTSDR
Sbjct: 128 VSADRAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHSTSDR 187

Query: 181 GGAMTAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWN 240
           GGAMTAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWN
Sbjct: 188 GGAMTAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWN 247

Query: 241 DWGDPTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLRPAPS 300
           DWGDPTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLRPAPS
Sbjct: 248 DWGDPTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLRPAPS 307

Query: 301 HFKGGFP 307
           HFKGGFP
Sbjct: 308 HFKGGFP 314

[Close]

BLAST of Cucsat.G3859 vs. NCBI nr
Match: KGN61801.1 (hypothetical protein Csa_006548 [Cucumis sativus])

HSP 1 Score: 635 bits (1639), Expect = 3.26e-230
Identity = 305/305 (100.00%), Postives = 305/305 (100.00%), Query Frame = 0


Query: 3   MAMGPGDMTTARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPED 62
           MAMGPGDMTTARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPED
Sbjct: 1   MAMGPGDMTTARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPED 60

Query: 63  KPYITISGSKASDTKITWNQGRDLLESPVVSIFASDFVGRFLTIENTFGTTGIAVALRVS 122
           KPYITISGSKASDTKITWNQGRDLLESPVVSIFASDFVGRFLTIENTFGTTGIAVALRVS
Sbjct: 61  KPYITISGSKASDTKITWNQGRDLLESPVVSIFASDFVGRFLTIENTFGTTGIAVALRVS 120

Query: 123 ADRAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHSTSDRGG 182
           ADRAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHSTSDRGG
Sbjct: 121 ADRAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHSTSDRGG 180

Query: 183 AMTAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDW 242
           AMTAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDW
Sbjct: 181 AMTAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDW 240

Query: 243 GDPTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLRPAPSHF 302
           GDPTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLRPAPSHF
Sbjct: 241 GDPTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLRPAPSHF 300

Query: 303 KGGFP 307
           KGGFP
Sbjct: 301 KGGFP 305

[Close]

BLAST of Cucsat.G3859 vs. NCBI nr
Match: XP_008460943.2 (PREDICTED: LOW QUALITY PROTEIN: putative pectinesterase 11 [Cucumis melo])

HSP 1 Score: 593 bits (1529), Expect = 1.72e-213
Identity = 285/298 (95.64%), Postives = 290/298 (97.32%), Query Frame = 0


Query: 6   GPGDMTTARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPEDKPY 65
           GP D  TARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPEDKPY
Sbjct: 3   GPRDKRTARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPEDKPY 62

Query: 66  ITISGSKASDTKITWNQGRDLLESPVVSIFASDFVGRFLTIENTFGTTGIAVALRVSADR 125
           ITISGSKASDT ITWNQGRDLL+SPVVSIFASDFVGRFLTIENTFGT+GIAVALRVSADR
Sbjct: 63  ITISGSKASDTIITWNQGRDLLQSPVVSIFASDFVGRFLTIENTFGTSGIAVALRVSADR 122

Query: 126 AAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHSTSDRGGAMT 185
           AAFYGCRIISFQDTLLDD+GRHYFNNCYIEGATDFICGNAASLYE+CHLHSTSDRGGAMT
Sbjct: 123 AAFYGCRIISFQDTLLDDSGRHYFNNCYIEGATDFICGNAASLYERCHLHSTSDRGGAMT 182

Query: 186 AQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDWGDP 245
           AQHRNT EENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDWGDP
Sbjct: 183 AQHRNTAEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDWGDP 242

Query: 246 TKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLRPAPSHFK 303
           TKQRTV YGEYKCYG GANR KRVVWSRSLSTDEA+KLFTKDIIGGRAWLRPAPSHFK
Sbjct: 243 TKQRTVSYGEYKCYGPGANRAKRVVWSRSLSTDEATKLFTKDIIGGRAWLRPAPSHFK 300

[Close]

BLAST of Cucsat.G3859 vs. NCBI nr
Match: XP_038902622.1 (putative pectinesterase 11 [Benincasa hispida])

HSP 1 Score: 571 bits (1472), Expect = 8.08e-205
Identity = 273/301 (90.70%), Postives = 285/301 (94.68%), Query Frame = 0


Query: 5   MGPGDMTTARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPEDKP 64
           M P DMT+ARLIRVD SGNGDFKKIQ+AIDSVPS NNELVFIWVKPGTYREKIVVPE+KP
Sbjct: 1   MAPTDMTSARLIRVDQSGNGDFKKIQEAIDSVPSHNNELVFIWVKPGTYREKIVVPEEKP 60

Query: 65  YITISGSKASDTKITWNQGRDLLESPVVSIFASDFVGRFLTIENTFGTTGIAVALRVSAD 124
           +ITISGSKASDT ITWNQG DLL+SPVVS+FASDF+GRFLTI+NTFGTTGIAVALRVSAD
Sbjct: 61  FITISGSKASDTIITWNQGTDLLQSPVVSVFASDFIGRFLTIQNTFGTTGIAVALRVSAD 120

Query: 125 RAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHSTSDRGGAM 184
           RAAFYGCRIISFQDTLLDD GRHYF NCYIEGATDFICGNAASLYE+CHLHSTSDRGGAM
Sbjct: 121 RAAFYGCRIISFQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYERCHLHSTSDRGGAM 180

Query: 185 TAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDWGD 244
           TAQHRNT EENTGFVFLGGKITGSGS FLGRPWGD SKVVFGYTYMSNVVEP+GWNDWGD
Sbjct: 181 TAQHRNTTEENTGFVFLGGKITGSGSTFLGRPWGDCSKVVFGYTYMSNVVEPQGWNDWGD 240

Query: 245 PTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLRPAPSHFKG 304
            +KQRTVLYGEYKCYG GANR KRV WSRSLSTDEA+KLFTKDIIGGRAWLRPAPSHFK 
Sbjct: 241 QSKQRTVLYGEYKCYGPGANRAKRVAWSRSLSTDEAAKLFTKDIIGGRAWLRPAPSHFKR 300

[Close]

BLAST of Cucsat.G3859 vs. NCBI nr
Match: XP_022947689.1 (putative pectinesterase 11 isoform X2 [Cucurbita moschata])

HSP 1 Score: 552 bits (1422), Expect = 4.20e-197
Identity = 262/299 (87.63%), Postives = 279/299 (93.31%), Query Frame = 0


Query: 5   MGPGDMTTARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPEDKP 64
           M P DM+TARLIRVD SG GDF+KIQ+AIDSVPS+NNELVFIW+KPGTYREKIVVPEDKP
Sbjct: 1   MAPADMSTARLIRVDQSGKGDFRKIQEAIDSVPSRNNELVFIWIKPGTYREKIVVPEDKP 60

Query: 65  YITISGSKASDTKITWNQGRDLLESPVVSIFASDFVGRFLTIENTFGTTGIAVALRVSAD 124
           +IT+SGSKASDT ITWNQGRDLL+SP +SIFA+DFVGRFLTI+NT+GTTGIAVALRVSAD
Sbjct: 61  FITLSGSKASDTIITWNQGRDLLQSPAISIFAADFVGRFLTIQNTYGTTGIAVALRVSAD 120

Query: 125 RAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHSTSDRGGAM 184
           RAAFYGCRIISFQDTLLDD GRHYF NCYIEGATDFICGNAASLYEKCHLHSTS+RGGAM
Sbjct: 121 RAAFYGCRIISFQDTLLDDVGRHYFKNCYIEGATDFICGNAASLYEKCHLHSTSERGGAM 180

Query: 185 TAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDWGD 244
           TAQHRNT  ENTGFVFLG KITGSGS  LGRPWGD S+VVFGYT+MSNVV PEGWNDWGD
Sbjct: 181 TAQHRNTVAENTGFVFLGCKITGSGSALLGRPWGDCSRVVFGYTHMSNVVVPEGWNDWGD 240

Query: 245 PTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLRPAPSHFK 303
            TKQRTV YGEYKCYG GANR KRVVWSRSLSTDEA+KLFTKD+IGGR WLRPAPSHFK
Sbjct: 241 HTKQRTVFYGEYKCYGPGANRAKRVVWSRSLSTDEAAKLFTKDMIGGRGWLRPAPSHFK 299

[Close]

BLAST of Cucsat.G3859 vs. ExPASy TrEMBL
Match: A0A0A0LP84 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_2G248110 PE=4 SV=1)

HSP 1 Score: 635 bits (1639), Expect = 1.58e-230
Identity = 305/305 (100.00%), Postives = 305/305 (100.00%), Query Frame = 0


Query: 3   MAMGPGDMTTARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPED 62
           MAMGPGDMTTARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPED
Sbjct: 1   MAMGPGDMTTARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPED 60

Query: 63  KPYITISGSKASDTKITWNQGRDLLESPVVSIFASDFVGRFLTIENTFGTTGIAVALRVS 122
           KPYITISGSKASDTKITWNQGRDLLESPVVSIFASDFVGRFLTIENTFGTTGIAVALRVS
Sbjct: 61  KPYITISGSKASDTKITWNQGRDLLESPVVSIFASDFVGRFLTIENTFGTTGIAVALRVS 120

Query: 123 ADRAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHSTSDRGG 182
           ADRAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHSTSDRGG
Sbjct: 121 ADRAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHSTSDRGG 180

Query: 183 AMTAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDW 242
           AMTAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDW
Sbjct: 181 AMTAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDW 240

Query: 243 GDPTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLRPAPSHF 302
           GDPTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLRPAPSHF
Sbjct: 241 GDPTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLRPAPSHF 300

Query: 303 KGGFP 307
           KGGFP
Sbjct: 301 KGGFP 305

[Close]

BLAST of Cucsat.G3859 vs. ExPASy TrEMBL
Match: A0A1S3CDL2 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499678 PE=4 SV=1)

HSP 1 Score: 593 bits (1529), Expect = 8.32e-214
Identity = 285/298 (95.64%), Postives = 290/298 (97.32%), Query Frame = 0


Query: 6   GPGDMTTARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPEDKPY 65
           GP D  TARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPEDKPY
Sbjct: 3   GPRDKRTARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPEDKPY 62

Query: 66  ITISGSKASDTKITWNQGRDLLESPVVSIFASDFVGRFLTIENTFGTTGIAVALRVSADR 125
           ITISGSKASDT ITWNQGRDLL+SPVVSIFASDFVGRFLTIENTFGT+GIAVALRVSADR
Sbjct: 63  ITISGSKASDTIITWNQGRDLLQSPVVSIFASDFVGRFLTIENTFGTSGIAVALRVSADR 122

Query: 126 AAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHSTSDRGGAMT 185
           AAFYGCRIISFQDTLLDD+GRHYFNNCYIEGATDFICGNAASLYE+CHLHSTSDRGGAMT
Sbjct: 123 AAFYGCRIISFQDTLLDDSGRHYFNNCYIEGATDFICGNAASLYERCHLHSTSDRGGAMT 182

Query: 186 AQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDWGDP 245
           AQHRNT EENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDWGDP
Sbjct: 183 AQHRNTAEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDWGDP 242

Query: 246 TKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLRPAPSHFK 303
           TKQRTV YGEYKCYG GANR KRVVWSRSLSTDEA+KLFTKDIIGGRAWLRPAPSHFK
Sbjct: 243 TKQRTVSYGEYKCYGPGANRAKRVVWSRSLSTDEATKLFTKDIIGGRAWLRPAPSHFK 300

[Close]

BLAST of Cucsat.G3859 vs. ExPASy TrEMBL
Match: A0A6J1G7K1 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111451478 PE=4 SV=1)

HSP 1 Score: 552 bits (1422), Expect = 2.03e-197
Identity = 262/299 (87.63%), Postives = 279/299 (93.31%), Query Frame = 0


Query: 5   MGPGDMTTARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPEDKP 64
           M P DM+TARLIRVD SG GDF+KIQ+AIDSVPS+NNELVFIW+KPGTYREKIVVPEDKP
Sbjct: 1   MAPADMSTARLIRVDQSGKGDFRKIQEAIDSVPSRNNELVFIWIKPGTYREKIVVPEDKP 60

Query: 65  YITISGSKASDTKITWNQGRDLLESPVVSIFASDFVGRFLTIENTFGTTGIAVALRVSAD 124
           +IT+SGSKASDT ITWNQGRDLL+SP +SIFA+DFVGRFLTI+NT+GTTGIAVALRVSAD
Sbjct: 61  FITLSGSKASDTIITWNQGRDLLQSPAISIFAADFVGRFLTIQNTYGTTGIAVALRVSAD 120

Query: 125 RAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHSTSDRGGAM 184
           RAAFYGCRIISFQDTLLDD GRHYF NCYIEGATDFICGNAASLYEKCHLHSTS+RGGAM
Sbjct: 121 RAAFYGCRIISFQDTLLDDVGRHYFKNCYIEGATDFICGNAASLYEKCHLHSTSERGGAM 180

Query: 185 TAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDWGD 244
           TAQHRNT  ENTGFVFLG KITGSGS  LGRPWGD S+VVFGYT+MSNVV PEGWNDWGD
Sbjct: 181 TAQHRNTVAENTGFVFLGCKITGSGSALLGRPWGDCSRVVFGYTHMSNVVVPEGWNDWGD 240

Query: 245 PTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLRPAPSHFK 303
            TKQRTV YGEYKCYG GANR KRVVWSRSLSTDEA+KLFTKD+IGGR WLRPAPSHFK
Sbjct: 241 HTKQRTVFYGEYKCYGPGANRAKRVVWSRSLSTDEAAKLFTKDMIGGRGWLRPAPSHFK 299

[Close]

BLAST of Cucsat.G3859 vs. ExPASy TrEMBL
Match: A0A6J1G7A7 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111451478 PE=4 SV=1)

HSP 1 Score: 547 bits (1410), Expect = 1.42e-195
Identity = 262/300 (87.33%), Postives = 279/300 (93.00%), Query Frame = 0


Query: 5   MGPGDMTTARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTY-REKIVVPEDK 64
           M P DM+TARLIRVD SG GDF+KIQ+AIDSVPS+NNELVFIW+KPGTY REKIVVPEDK
Sbjct: 1   MAPADMSTARLIRVDQSGKGDFRKIQEAIDSVPSRNNELVFIWIKPGTYSREKIVVPEDK 60

Query: 65  PYITISGSKASDTKITWNQGRDLLESPVVSIFASDFVGRFLTIENTFGTTGIAVALRVSA 124
           P+IT+SGSKASDT ITWNQGRDLL+SP +SIFA+DFVGRFLTI+NT+GTTGIAVALRVSA
Sbjct: 61  PFITLSGSKASDTIITWNQGRDLLQSPAISIFAADFVGRFLTIQNTYGTTGIAVALRVSA 120

Query: 125 DRAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHSTSDRGGA 184
           DRAAFYGCRIISFQDTLLDD GRHYF NCYIEGATDFICGNAASLYEKCHLHSTS+RGGA
Sbjct: 121 DRAAFYGCRIISFQDTLLDDVGRHYFKNCYIEGATDFICGNAASLYEKCHLHSTSERGGA 180

Query: 185 MTAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDWG 244
           MTAQHRNT  ENTGFVFLG KITGSGS  LGRPWGD S+VVFGYT+MSNVV PEGWNDWG
Sbjct: 181 MTAQHRNTVAENTGFVFLGCKITGSGSALLGRPWGDCSRVVFGYTHMSNVVVPEGWNDWG 240

Query: 245 DPTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLRPAPSHFK 303
           D TKQRTV YGEYKCYG GANR KRVVWSRSLSTDEA+KLFTKD+IGGR WLRPAPSHFK
Sbjct: 241 DHTKQRTVFYGEYKCYGPGANRAKRVVWSRSLSTDEAAKLFTKDMIGGRGWLRPAPSHFK 300

[Close]

BLAST of Cucsat.G3859 vs. ExPASy TrEMBL
Match: A0A6J1KXA2 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111499612 PE=4 SV=1)

HSP 1 Score: 544 bits (1401), Expect = 3.11e-194
Identity = 257/299 (85.95%), Postives = 275/299 (91.97%), Query Frame = 0


Query: 5   MGPGDMTTARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPEDKP 64
           M P DM+TARLIRVD SG GDF+KIQ+AIDSVP +NNELVFIW+KPGTYREKIVVPEDKP
Sbjct: 1   MAPADMSTARLIRVDQSGKGDFRKIQEAIDSVPPRNNELVFIWIKPGTYREKIVVPEDKP 60

Query: 65  YITISGSKASDTKITWNQGRDLLESPVVSIFASDFVGRFLTIENTFGTTGIAVALRVSAD 124
           +IT+SGSKASDT ITWNQGRDLL+SP +S+FASDFV RFLTI+NT+GTTGIAVALRVSAD
Sbjct: 61  FITLSGSKASDTIITWNQGRDLLQSPAISVFASDFVARFLTIQNTYGTTGIAVALRVSAD 120

Query: 125 RAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHSTSDRGGAM 184
           RAAFYGCRIISFQDTLLDD GRHYF NCYIEGATDFICGNAASLYEKCHLHS S+RGGAM
Sbjct: 121 RAAFYGCRIISFQDTLLDDVGRHYFKNCYIEGATDFICGNAASLYEKCHLHSMSERGGAM 180

Query: 185 TAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDWGD 244
           TAQHRNT  ENTGFVFLG KITGSGS  LGRPWGD S+VVFGYT+MSNVV PEGWNDWGD
Sbjct: 181 TAQHRNTTAENTGFVFLGCKITGSGSALLGRPWGDCSRVVFGYTHMSNVVVPEGWNDWGD 240

Query: 245 PTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLRPAPSHFK 303
            TKQRTV YGEYKCYG G+NR KRV WSRSLSTDEA+KLFTKD+IGGR WLRPAPSHFK
Sbjct: 241 HTKQRTVFYGEYKCYGPGSNRAKRVAWSRSLSTDEAAKLFTKDMIGGRGWLRPAPSHFK 299

[Close]

BLAST of Cucsat.G3859 vs. TAIR 10
Match: AT2G21610.1 (pectinesterase 11 )

HSP 1 Score: 434.5 bits (1116), Expect = 7.1e-122
Identity = 198/297 (66.67%), Postives = 242/297 (81.48%), Query Frame = 0


Query: 9   DMTTARLIRVDGSGNGDFKKIQQAIDSVPS--QNNELVFIWVKPGTYREKIVVPEDKPYI 68
           D +TA LIRVD SG GDF KIQ+AI+S+P    N++L FIWVKPG YREK+V+P +KPYI
Sbjct: 44  DFSTAILIRVDQSGKGDFSKIQEAIESIPPNLNNSQLYFIWVKPGIYREKVVIPAEKPYI 103

Query: 69  TISGSKASDTKITWNQGRDLLESPVVSIFASDFVGRFLTIENTFGTTGIAVALRVSADRA 128
           T+SG++AS+T + W+ G D+LESP ++IFASDFV RFLTI+N FGT G AVALRV+AD+A
Sbjct: 104 TLSGTQASNTFLIWSDGEDILESPTLTIFASDFVCRFLTIQNKFGTAGRAVALRVAADKA 163

Query: 129 AFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHSTSDRGGAMTA 188
           AFYGC I S+QDTLLDD G HYF NCYIEGATDFICG+A+SLYE+CHLHS S   G++TA
Sbjct: 164 AFYGCVITSYQDTLLDDNGNHYFKNCYIEGATDFICGSASSLYERCHLHSLSPNNGSITA 223

Query: 189 QHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDWGDPT 248
           Q R +  E +GF FLG K+TGSGS FLGRPWG +S+VVF Y++ SNVV P+GWN WGD T
Sbjct: 224 QMRTSATEKSGFTFLGCKLTGSGSTFLGRPWGAYSRVVFAYSFFSNVVAPQGWNQWGDST 283

Query: 249 KQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLRPAPSHFK 304
           K+ TV YGEYKCYG GA+R++RV WS+ LS +EA+   +KD IGG+ WLRPAPSHFK
Sbjct: 284 KENTVYYGEYKCYGPGADREQRVEWSKQLSDEEATVFLSKDFIGGKDWLRPAPSHFK 340

[Close]

BLAST of Cucsat.G3859 vs. TAIR 10
Match: AT5G19730.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 277.3 bits (708), Expect = 1.5e-74
Identity = 140/292 (47.95%), Postives = 186/292 (63.70%), Query Frame = 0


Query: 21  SGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPEDKPYITISGSKASDTKITW 80
           S  GDF KIQ AIDS+P  N   V I V  G Y+EK+ +P  K +ITI G  A  T + W
Sbjct: 91  SNKGDFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTVEW 150

Query: 81  N---QGRDLLESPV-------VSIFASDFVGRFLTIENTF-----GTTG-IAVALRVSAD 140
               Q  D   +P+        ++ +  FV + +T  NT      G  G  AVALRVSAD
Sbjct: 151 GDTAQTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSAD 210

Query: 141 RAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHSTSDRGGAM 200
            AAF+GCR++  QDTL D  GRHY+ +CYIEG+ DFI GNA SLYE CH+H+ +D+ GA+
Sbjct: 211 NAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKLGAV 270

Query: 201 TAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDWGD 260
           TAQ R++  E+TGF F+  K+TG+G ++LGR WG FS+VVF YTYM N++ P GW +WGD
Sbjct: 271 TAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWYNWGD 330

Query: 261 PTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLR 297
           P+++ TV YG+YKC G GAN   RV W+R L+ +EA    +   I G  W++
Sbjct: 331 PSREMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFLSLTFIDGSEWIK 382

[Close]

BLAST of Cucsat.G3859 vs. TAIR 10
Match: AT5G55590.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 265.8 bits (678), Expect = 4.4e-71
Identity = 133/300 (44.33%), Postives = 188/300 (62.67%), Query Frame = 0


Query: 14  RLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPEDKPYITISG--S 73
           R+I VD +G GD   +Q A+D VP  N++ V I++ PG YREK++VP+ KPYI+  G  S
Sbjct: 80  RVIVVDKNGGGDSVTVQGAVDMVPDSNSQRVKIFILPGIYREKVIVPKSKPYISFIGNES 139

Query: 74  KASDTKITWN--------QGRDL--LESPVVSIFASDFVGRFLTIENTF----GTTG-IA 133
            A DT I+W+         G++L    +  VSI +  F    +T ENT     G  G  A
Sbjct: 140 YAGDTVISWSDKASDLGCDGKELGTYRTASVSIESDFFCATAITFENTVVAEAGEQGRQA 199

Query: 134 VALRVSADRAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHS 193
           VALR+  D+A FY  R++  QDTL DD G HYF  CYI+G  DFI GNA SLY+ C +HS
Sbjct: 200 VALRIIGDKAVFYRVRVLGSQDTLFDDNGSHYFYQCYIQGNVDFIFGNAKSLYQDCDIHS 259

Query: 194 TSDRGGAMTAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEP 253
           T+ R GA+ A HR++  E+TGF F+   I+G+G ++LGR WG++S+ V+   ++++++ P
Sbjct: 260 TAKRYGAIAAHHRDSETEDTGFSFVNCDISGTGQIYLGRAWGNYSRTVYSNCFIADIITP 319

Query: 254 EGWNDWGDPTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLR 297
            GW+DW  P +QR V++GEY C G GA R  RV WS++L+ DE      ++ I G  WLR
Sbjct: 320 VGWSDWKHPERQRKVMFGEYNCRGRGAERGGRVPWSKTLTRDEVKPFLGREFIYGDQWLR 379

[Close]

BLAST of Cucsat.G3859 vs. TAIR 10
Match: AT2G36710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 251.1 bits (640), Expect = 1.1e-66
Identity = 128/300 (42.67%), Postives = 179/300 (59.67%), Query Frame = 0


Query: 12  TARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPEDKPYITISGS 71
           T+ ++ VD  G G+F  +Q AID VP  ++    I V  G+YREK+ V E+K  + I G 
Sbjct: 87  TSLVLTVDLHGCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENKTNLVIQGR 146

Query: 72  KASDTKITWN----QGRDLLESPVVSIFASDFVGRFLTIENTF------GTTGIAVALRV 131
              +T I WN       +  +S    +FA++F    ++ +N             AVALR+
Sbjct: 147 GYQNTSIEWNDTAKSAGNTADSFSFVVFAANFTAYNISFKNNAPEPDPGEADAQAVALRI 206

Query: 132 SADRAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHS----- 191
             D+AAFYGC     QDTLLDD GRH+F  C+I+G+ DFI GN  SLY+ C ++S     
Sbjct: 207 EGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRSLYQDCTINSIAKGN 266

Query: 192 TSDRGGAMTAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEP 251
           TS   G++TAQ R + +E +GF F+  KI GSG + LGR WG ++ VVF  TYMS ++ P
Sbjct: 267 TSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGRAWGAYATVVFSNTYMSGIITP 326

Query: 252 EGWNDWGDPTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLR 297
           EGWN+WGD TK++TV +GE+KCYG GA+  +RV++ + L+  EAS       I G  WLR
Sbjct: 327 EGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFGKQLTDSEASSFIDVSFIDGDEWLR 386

[Close]

BLAST of Cucsat.G3859 vs. TAIR 10
Match: AT5G47500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 250.4 bits (638), Expect = 1.9e-66
Identity = 123/301 (40.86%), Postives = 176/301 (58.47%), Query Frame = 0


Query: 14  RLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPEDKPYITISGSKA 73
           ++I V  +G+  F+ +Q A+DS+P  NN+ + I + PG YREK+VVP  KPYIT  G+  
Sbjct: 59  KVITVSLNGHAQFRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGR 118

Query: 74  SDTKITWN--------QGRDL--LESPVVSIFASDFVGRFLTIENTF-----GTTG-IAV 133
             T I W+         G+ L   ++  V+++A+ F  R ++  NT      G  G  AV
Sbjct: 119 DVTAIEWHDRASDLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQAV 178

Query: 134 ALRVSADRAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHST 193
           A R+S D+A F GC     QDTL DD GRHYF  CYIEG+ DFI GN  S+Y+ C LHS 
Sbjct: 179 AFRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSI 238

Query: 194 SDRGGAMTAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPE 253
           + R G++ A  R   EE TGF F+G ++TG+G +++GR  G +S++V+ YTY   +V   
Sbjct: 239 ASRFGSIAAHGRTCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYFDALVAHG 298

Query: 254 GWNDWGDPT-KQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLR 298
           GW+DW   + K +T  +G Y CYG GA   + V W+R+L  + A     K  + GR W+ 
Sbjct: 299 GWDDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSFVNGRHWIA 358

The following BLAST results are available for this feature:

BLAST of Cucsat.G3859 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SIJ9	1.0e-120	66.67	Putative pectinesterase 11 OS=Arabidopsis thaliana OX=3702 GN=PME11 PE=3 SV=1	[more]
Q8VYZ3	2.1e-73	47.95	Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1	[more]
Q9FM79	6.2e-70	44.33	Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=2 SV=1	[more]
Q9ZQA3	1.6e-65	42.67	Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1	[more]
Q8LPF3	2.7e-65	40.86	Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1	[more]

BLAST of Cucsat.G3859 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004148862.1	6.76e-232	100.00	putative pectinesterase 11 [Cucumis sativus]	[more]
KGN61801.1	3.26e-230	100.00	hypothetical protein Csa_006548 [Cucumis sativus]	[more]
XP_008460943.2	1.72e-213	95.64	PREDICTED: LOW QUALITY PROTEIN: putative pectinesterase 11 [Cucumis melo]	[more]
XP_038902622.1	8.08e-205	90.70	putative pectinesterase 11 [Benincasa hispida]	[more]
XP_022947689.1	4.20e-197	87.63	putative pectinesterase 11 isoform X2 [Cucurbita moschata]	[more]

BLAST of Cucsat.G3859 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LP84	1.58e-230	100.00	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_2G248110 PE=4 SV=1	[more]
A0A1S3CDL2	8.32e-214	95.64	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499678 PE=4 SV=1	[more]
A0A6J1G7K1	2.03e-197	87.63	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111451478 PE=4 SV=1	[more]
A0A6J1G7A7	1.42e-195	87.33	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111451478 PE=4 SV=1	[more]
A0A6J1KXA2	3.11e-194	85.95	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111499612 PE=4 SV=1	[more]

BLAST of Cucsat.G3859 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G21610.1	7.1e-122	66.67	pectinesterase 11	[more]
AT5G19730.1	1.5e-74	47.95	Pectin lyase-like superfamily protein	[more]
AT5G55590.1	4.4e-71	44.33	Pectin lyase-like superfamily protein	[more]
AT2G36710.1	1.1e-66	42.67	Pectin lyase-like superfamily protein	[more]
AT5G47500.1	1.9e-66	40.86	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 1..122 e-value: 1.1E-32 score: 115.6
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 1..104 e-value: 8.6E-23 score: 80.5
None	No IPR available	PANTHER	PTHR31321:SF94	BNAA04G12290D PROTEIN	coord: 1..120
None	No IPR available	PANTHER	PTHR31321	ACYL-COA THIOESTER HYDROLASE YBHC-RELATED	coord: 1..120
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 2..120

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G3859.T4	Cucsat.G3859.T4	mRNA
Cucsat.G3859.T1	Cucsat.G3859.T1	mRNA
Cucsat.G3859.T3	Cucsat.G3859.T3	mRNA
Cucsat.G3859.T2	Cucsat.G3859.T2	mRNA
Cucsat.G3859.T5	Cucsat.G3859.T5	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0045490	pectin catabolic process
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity