Cucsat.G21422 (gene) Cucumber (B10) v3

Overview
NameCucsat.G21422
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Descriptionalpha/beta-Hydrolases superfamily protein
Locationctg910: 3068782 .. 3073028 (+)
RNA-Seq ExpressionCucsat.G21422
SyntenyCucsat.G21422
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGGGTTAACGGTCATAAACTATTTTAGAGAAAAAAACGTGTCATTGTGGATTGGTCACGTCCAAATTTCACTCTCTTCAAGTTCATACACACACACATACTAATCCAATAATAATTCCCAATTTTCTCCTAATTTTTTCTCCCCATCTCAACAATCCAATGCACCATGTTCTTCCCCAGATCCAATTTTCCCCTAAATTTCATTCAATCTCCCAAATTCGTAACCCCAATTCCAAATACAGTCGTTGACTTCCAGGTATAATCAATCCCTTTTCTATCTTTAGCCTTTATATGCCATTTCGGATTTGGGTTTTCAATTCTGCGTATGGCACTCAACAAACCTTACAGCTAACTTCACAGCATTGACTCGTTTTCTCCGTCTCCATTGTTAATTTTAAGAAATGCCCCCCTGTTGTCAAACATTGGAATCCCATTTTTTAGCTAAAGAATATTCTCTATACGATTCTGATTCTTGTATTCTTAACCGGAAGTGCGCATTTGGGTTTTGTTCTTTGCTCTTGATGATCCAAATTTGAAACCCAGATGTTCTTTTTTGCTTTTTGTTGTCTTGTTTGTGCCTATATTAGTTTACCTTTTATGCTCTTCAGTGATTGGAAGCTACTTGAAGGTTTATATGGTATTGGTTGTTGAATGAATTCCCTCATTCAAGTTTTGGTGTCCTACTCTTAAAAGTTATTGTTTGTCATTATTGAGATTGTTTTTTCTTGGGGATTGTGCCTTTTAGTTCAATGATTGGTCAAAATCCTTTGTTGTTCTTCCATCGTCATTGTCTTTTACACCTATTATCAACTCCAGGACTTGTATCGTTAGAGCTTGTAGTTTCCTAGTGATAATATATTTCTCCACAACTCGTGCATTTACTAGGAAAATCTGTTCCATATAGTGTTCCTTCGAGCTTAGATCATAGGCACCAATGTCTATCTTATGTGGCGTGCCTATCCTTGAGTGTGTATGCTGTCTGGGTTGTGCTCGTTGGGTCTGGAAGCGGTGTCTCCACACAGCTGGTCATGACAGTGAAAATTGGGGATTTGCTACTCCTGATGAATTCGAGCCTATTCCCCGAATTTGTCGATATATCCTATCTGTGTATGAAGATGATATTCGAAAACCTCTTTGGGAACCGGTTGGTGGGTATGGAATCAATCCTGATTGGTTACTCATGAAGAAGACATACAAAGATACACGAGGACGAGCTCCTCCATATATTTTATATCTTGATCACGTTCATGCCGATATTGTCCTTGCCATCAGGGGACTTAATATGGCAAAGGAGAGTGATTATGCAGTTTTATTGGACAACAGGCTGGGAAAGAAGAAATTTGATGGTGGATATGTTCACAATGGGCTTCTGAAGGCAGCTGGTTGGGTTTTGGACACTGAGAACGAAACTTTAAAGGATTTGGTGAAGAAGTATCCAGATTATACCTTGACGTTTGCAGGGCATTCTCTTGGCTCTGGAGTAGCAGCCATGCTAACATTACTAGTAGTACAAAATCGCGAAAAATTGGAAAATATTGATCGGAAACGGATAAGGTGTTTTGCTATTGCTCCTGCCAGATGCATGTCCCTAAACTTGGCTGTTAGATATGCAGATGTTATCAACTCTGTTGTTCTTCAGGTAACCTATACATTCTTCCTTTATTTTATTTGTAAAGTGATCAAAACCATTTTGGGAGCGATTAAAATAATGTAGATTATGCATTATGAGACCTCAATAACTCCTAGGATTTGTTTGTGAATCTCTCATATAACATTTCCTCAGTCTTTACATCAACCTTCTGTTGTTCCCAACGTTTTTTTTCTCCTCTTTAGAATCATTTTCTTAATAAATTTATATTGTGTATTCAGTAAAACAGAAACGTTTTGCTTTTTGTTTGATGTGCACGAACTCTTGACTGTTTGAAAATACTATTGATTCTCATTTTTTAAAGTGTGCTGAATATGGTGGAATTTATACTTACATTTCGAAATATAACGGTGATTGTTATACTGAACCAGGATGACTTTTTACCCCGGACAGCCACACCATTGGAAGACATTTTTAAATCACTCTTCTGGTATGGTTCAAGATTCATGACCCTTTATGGTGTTAACTCTTGTTGCGTAGTTGTCAACAATCATTTTCAAAGTAGATAATTCTGCCAAAATTGCAAATTAGATCTCTATTATTGGAATGAGCACTGCTTCTTATGTTAAAGTTCATTGTATAAAAGCAATAACAGAAAAATGGTCTTGGATTTATTCAGTGCAATTTTAAGTTTCTCAATAATCATTGGTTCTTTTCTCCTTGTAGTTTGCCGTGCCTTCTATGCCTGAGATGTGTTCGGGATACATGTGTATCAGAAGAAAAGATGCTCAAAGATCCAAGGAGACTTTATGCACCTGGTCGACTCTATCACATTGTAGAGCGAAAACCCTTCAGGTAAAAGCCCTATATCTTCTATGTTAAGCTGGTTAATGTGGTGGAGAAATCTTGTGTACCGTGAAGTTTGTGAAATTTTTTTCATGTTTCGTTCAAACAAGAAAACATACCTGTAAAACTGCTTGTTGTGTGGAAAGAAACCATCCTTGTATCACGAGCATCTGTTAAAAGGGTTGAGAGACTCTGAGTTTAAGTCCCTCCTGTACTTGTTGTGTGGAAAGAAAGCCTCTCTTAACCTTACTAGTTATTGAAATGTTGGCTTTCTAGACCATTGAAAAAGAAACATGCCATGCTCAAACATTTGACTGAATTCACCTCTTTTTTGCTGAAAGATGCGGAAGGTTTCCACCAGTCGTGAAGACGGCTGTTCCAGTTGATGGGAGGTTTGAACACATAGTTCTTTCTTGTAATGCAACTTCAGACCATGCTATCATTTGGATAGAGAAAGAAGCCAAATGGGCCCTGGAAGTAAGTTTCATCTTTCTACCGAGCCTTTTATGCCGAGTGTTTGATTTTCAATGGAAATTTTGGAAGACATTCTTTTGCATTTCTTTATTCTACATTTTCGTTTGTGGTTCTTCTTTGCCTTTCTCTAGTTGTCTATGAAGTGGAGGATCAGATTCTAATTCGTCCCGTACACGAGAACTGCCAAAATAGAATTCTAATTCTTTTAGGTTTATTGACTCAATTTATTCTTTCCCTTACCTACTCTTAGATGTGAATTAGAGTTCATTGGTTGGTTTTGACCTGAATTCTTCTCGTAACACCAAAATTATTGTTAAACATGTTCTTCCCTCGGTTACCCGAAAATCAAGAGAAACTATCTGATCCATATTGTTTATTTTAGATTCAGAGTAGAGAGTACAAAAACGAATGTTTTCTTTCTGTACCGTAAAAGCTTAAGATACATTATTATTCAAAACAAGGAATTAGACCATCTAATGACTAACATGAAGAGAATCATCAGTCTCCCAAATTTAAAATGAGTAACTTTTAGTTAAGATCTTGTTAATTGAATGTATTCTTGTCATAATTTACAAGTGAGCTCTATGAAATCATTGCCTGAAAAACGAAAAACAACATCCATTTGATTCTGTCAATTTCAGTTAATGCGCAAGGATGATAAGATCATGGAGATACCACCCCAACAGAAGATGGAGAGGCAACAGACTTTAGCCAGAGAGCACAGTGAAGAGTACAAGGCTGCATTGCAGCGGGCCGTGACATTAGCCGTGCCACATGCCTACGCAATTTCCCCATACGGAACCTTCAGCCAAACAGATGAAGGGGAAGAAGAAAAGTCACCAGCATCGAGTGGAGGGTCATCGAGGAGGAGGAAAGAAACTTGGGATGAATTGATTGAGCGTCTATATGACAAGGATGATTCAAGACACACAGTCCTGAAGAAATCACTTAGTTGTATTTAACATTATTCTTTCATATCAATACTTATTACATGGAGTTTCCTCAGATGAATCTACGGTACGATGTCAGATTACCTTTGAGGATGCTGATTTCCTTGGCTTCAATTGTTTGAAGATGATGGAAAATATGCAGTGTTCATCCAATTTTTTAGAAGTGTGATGAAGTTCAGCTATATTTTTGATGTGCTACTTGTAAGTTTCTTCATTGTAGGAAGTGAATATTTATATTGGTAGTATGTAAAGGTAGCTTGCCTTTTCTCTTCTGTTGGATAGCACATTATATACCATTGTTCAAATGTTGAAAAACTATATAGCAACAATAGCTCTACGCAATAGTGTGTGATAAAAGCTTGACCACTCTCATCAAGTAATTGAAGATC

Coding sequence (CDS)

ATGTCTATCTTATGTGGCGTGCCTATCCTTGAGTGTGTATGCTGTCTGGGTTGTGCTCGTTGGGTCTGGAAGCGGTGTCTCCACACAGCTGGTCATGACAGTGAAAATTGGGGATTTGCTACTCCTGATGAATTCGAGCCTATTCCCCGAATTTGTCGATATATCCTATCTGTGTATGAAGATGATATTCGAAAACCTCTTTGGGAACCGGTTGGTGGGTATGGAATCAATCCTGATTGGTTACTCATGAAGAAGACATACAAAGATACACGAGGACGAGCTCCTCCATATATTTTATATCTTGATCACGTTCATGCCGATATTGTCCTTGCCATCAGGGGACTTAATATGGCAAAGGAGAGTGATTATGCAGTTTTATTGGACAACAGGCTGGGAAAGAAGAAATTTGATGGTGGATATGTTCACAATGGGCTTCTGAAGGCAGCTGGTTGGGTTTTGGACACTGAGAACGAAACTTTAAAGGATTTGGTGAAGAAGTATCCAGATTATACCTTGACGTTTGCAGGGCATTCTCTTGGCTCTGGAGTAGCAGCCATGCTAACATTACTAGTAGTACAAAATCGCGAAAAATTGGAAAATATTGATCGGAAACGGATAAGGTGTTTTGCTATTGCTCCTGCCAGATGCATGTCCCTAAACTTGGCTGTTAGATATGCAGATGTTATCAACTCTGTTGTTCTTCAGGATGACTTTTTACCCCGGACAGCCACACCATTGGAAGACATTTTTAAATCACTCTTCTGTTTGCCGTGCCTTCTATGCCTGAGATGTGTTCGGGATACATGTGTATCAGAAGAAAAGATGCTCAAAGATCCAAGGAGACTTTATGCACCTGGTCGACTCTATCACATTGTAGAGCGAAAACCCTTCAGATGCGGAAGGTTTCCACCAGTCGTGAAGACGGCTGTTCCAGTTGATGGGAGGTTTGAACACATAGTTCTTTCTTGTAATGCAACTTCAGACCATGCTATCATTTGGATAGAGAAAGAAGCCAAATGGGCCCTGGAATTAATGCGCAAGGATGATAAGATCATGGAGATACCACCCCAACAGAAGATGGAGAGGCAACAGACTTTAGCCAGAGAGCACAGTGAAGAGTACAAGGCTGCATTGCAGCGGGCCGTGACATTAGCCGTGCCACATGCCTACGCAATTTCCCCATACGGAACCTTCAGCCAAACAGATGAAGGGGAAGAAGAAAAGTCACCAGCATCGAGTGGAGGGTCATCGAGGAGGAGGAAAGAAACTTGGGATGAATTGATTGAGCGTCTATATGACAAGGATGATTCAAGACACACAGTCCTGAAGAAATCACTTAGTTGTATTTAA

Protein sequence

MSILCGVPILECVCCLGCARWVWKRCLHTAGHDSENWGFATPDEFEPIPRICRYILSVYEDDIRKPLWEPVGGYGINPDWLLMKKTYKDTRGRAPPYILYLDHVHADIVLAIRGLNMAKESDYAVLLDNRLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLGSGVAAMLTLLVVQNREKLENIDRKRIRCFAIAPARCMSLNLAVRYADVINSVVLQDDFLPRTATPLEDIFKSLFCLPCLLCLRCVRDTCVSEEKMLKDPRRLYAPGRLYHIVERKPFRCGRFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMRKDDKIMEIPPQQKMERQQTLAREHSEEYKAALQRAVTLAVPHAYAISPYGTFSQTDEGEEEKSPASSGGSSRRRKETWDELIERLYDKDDSRHTVLKKSLSCI
Homology
BLAST of Cucsat.G21422 vs. ExPASy Swiss-Prot
Match: Q8NCG7 (Diacylglycerol lipase-beta OS=Homo sapiens OX=9606 GN=DAGLB PE=1 SV=2)

HSP 1 Score: 58.5 bits (140), Expect = 2.2e-07
Identity = 47/164 (28.66%), Postives = 74/164 (45.12%), Query Frame = 0

Query: 96  PYILYLDHVHADIVLAIRGLNMAKESDYAVLLDNRLGKKKFD------GGYVHNGLLKAA 155
           P+++ LDH    +V+A+RG    ++    VL D     +  D          H G+ +AA
Sbjct: 357 PFLVALDHRKESVVVAVRGTMSLQD----VLTDLSAESEVLDVECEVQDRLAHKGISQAA 416

Query: 156 GWVLD--TENETLKDLVKKYPDYTLTFAGHSLGSGVAAMLTLLVVQNREKLENIDRKRIR 215
            +V      +  L       P+Y L   GHSLG G AA+L  ++             ++R
Sbjct: 417 RYVYQRLINDGILSQAFSIAPEYRLVIVGHSLGGGAAALLATML--------RAAYPQVR 476

Query: 216 CFAIAPARCMSLNLAVRYA-DVINSVVLQDDFLPR-TATPLEDI 250
           C+A +P R +       Y+   I S+VL  D +PR + T LED+
Sbjct: 477 CYAFSPPRGLWSKALQEYSQSFIVSLVLGKDVIPRLSVTNLEDL 508

BLAST of Cucsat.G21422 vs. ExPASy Swiss-Prot
Match: Q91WC9 (Diacylglycerol lipase-beta OS=Mus musculus OX=10090 GN=Daglb PE=1 SV=2)

HSP 1 Score: 57.0 bits (136), Expect = 6.4e-07
Identity = 45/164 (27.44%), Postives = 78/164 (47.56%), Query Frame = 0

Query: 96  PYILYLDHVHADIVLAIRGLNMAKESDYAVLLDNRLGKKKFDGG------YVHNGLLKAA 155
           P+I+ LDH    +V+A+RG    ++    VL D     +  + G        H G+ +AA
Sbjct: 357 PFIVVLDHRKESVVVAVRGTMSLQD----VLTDLSAESETLELGIELQDCVAHKGIAQAA 416

Query: 156 GWVLD--TENETLKDLVKKYPDYTLTFAGHSLGSGVAAMLTLLVVQNREKLENIDRKRIR 215
            ++      +  L       P+Y L   GHSLG+G AA+L +++             ++R
Sbjct: 417 RYIHRRLVNDGILSQAFSVAPEYQLVLVGHSLGAGAAALLAIML--------RGAYPQVR 476

Query: 216 CFAIAPAR-CMSLNLAVRYADVINSVVLQDDFLPR-TATPLEDI 250
            +A +P R  +S +L     D + S++L  D +PR + T +ED+
Sbjct: 477 AYAFSPPRGLLSKSLYEYSKDFVVSLILGMDVIPRLSVTNMEDL 508

BLAST of Cucsat.G21422 vs. ExPASy Swiss-Prot
Match: P0C1S9 (Diacylglycerol lipase-beta OS=Rattus norvegicus OX=10116 GN=Daglb PE=1 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 8.3e-07
Identity = 42/160 (26.25%), Postives = 77/160 (48.12%), Query Frame = 0

Query: 96  PYILYLDHVHADIVLAIRGLNMAKE--SDYAVLLDNRLGKKKFDGGYVHNGLLKAAGWVL 155
           P+I+ LDH    +V+A+RG    ++  +D +   +N     +      H G+ +AA ++ 
Sbjct: 357 PFIVVLDHRKESVVVAVRGTMSLQDVLTDLSAESENLELDIELQDCVAHKGIAQAARYIY 416

Query: 156 D--TENETLKDLVKKYPDYTLTFAGHSLGSGVAAMLTLLVVQNREKLENIDRKRIRCFAI 215
                +  L       P+Y L   GHSLG+G AA+L +++             ++R +A 
Sbjct: 417 RRLVNDGILSQAFSVAPEYRLVVVGHSLGAGAAALLAIML--------RGAYPQVRAYAF 476

Query: 216 APAR-CMSLNLAVRYADVINSVVLQDDFLPR-TATPLEDI 250
           +P R  +S +L     D + S++L  D +PR +   +ED+
Sbjct: 477 SPPRGLLSKSLFEYSKDFVVSLILGMDVIPRLSVANMEDL 508

BLAST of Cucsat.G21422 vs. ExPASy Swiss-Prot
Match: Q5YLM1 (Diacylglycerol lipase-alpha OS=Rattus norvegicus OX=10116 GN=Dagla PE=1 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 1.2e-05
Identity = 59/242 (24.38%), Postives = 102/242 (42.15%), Query Frame = 0

Query: 96  PYILYLDHVHADIVLAIRGLNMAKESDYAVLLD-NRLGKKKFDGGYV-HNGLLKAAGWVL 155
           P+ + +DH    +V++IRG    K++   +  D  RL  +   G ++ H G++ +A ++ 
Sbjct: 381 PFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHRGTWLGHKGMVLSAEYIK 440

Query: 156 DTENETL-------KDLVKKYPDYTLTFAGHSLGSGVAAMLTLLVVQNREKLENIDRKRI 215
               + +       +DL +    Y L   GHSLG+G AA+L+ L+      L        
Sbjct: 441 KKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLRPQYPTL-------- 500

Query: 216 RCFAIAPARCMSLNLAVRYA-DVINSVVLQDDFLPRTA-TPLEDIFKSLF---------- 275
           +CFA +P   +    A+ Y+ + + +VVL  D +PR   + LE   + L           
Sbjct: 501 KCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQLLDVLQRSTKPK 560

Query: 276 ---------CLP-CLLCLRCVRDTCVSEEKMLKDP----------RRLYAPGRLYHIVER 297
                    C+P   L    V  T ++  ++   P            LY PGR+ H+V  
Sbjct: 561 WRIIVGATKCIPKSELPEDQVEVTALASTRLWTHPSDLTIALSASTPLYPPGRIIHVVHN 614

BLAST of Cucsat.G21422 vs. ExPASy Swiss-Prot
Match: Q6WQJ1 (Diacylglycerol lipase-alpha OS=Mus musculus OX=10090 GN=Dagla PE=1 SV=2)

HSP 1 Score: 52.4 bits (124), Expect = 1.6e-05
Identity = 59/242 (24.38%), Postives = 102/242 (42.15%), Query Frame = 0

Query: 96  PYILYLDHVHADIVLAIRGLNMAKESDYAVLLD-NRLGKKKFDGGYV-HNGLLKAAGWVL 155
           P+ + +DH    +V++IRG    K++   +  D  RL  +   G ++ H G++ +A ++ 
Sbjct: 381 PFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHRGTWLGHKGMVLSAEYIK 440

Query: 156 DTENETL-------KDLVKKYPDYTLTFAGHSLGSGVAAMLTLLVVQNREKLENIDRKRI 215
               + +       +DL +    Y L   GHSLG+G AA+L+ L+      L        
Sbjct: 441 KKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLRPQYPTL-------- 500

Query: 216 RCFAIAPARCMSLNLAVRYA-DVINSVVLQDDFLPRTA-TPLEDIFKSLF---------- 275
           +CFA +P   +    A+ Y+ + + +VVL  D +PR   + LE   + L           
Sbjct: 501 KCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQLLDVLQRSTKPK 560

Query: 276 ---------CLP-CLLCLRCVRDTCVSEEKMLKDP----------RRLYAPGRLYHIVER 297
                    C+P   L    V  T ++  ++   P            LY PGR+ H+V  
Sbjct: 561 WRIIVGATKCIPKSELPEDQVEVTTLASTRLWTHPSDLTIALSASTPLYPPGRIIHVVHN 614

BLAST of Cucsat.G21422 vs. NCBI nr
Match: XP_004152416.1 (uncharacterized protein LOC101207943 [Cucumis sativus] >XP_011654833.1 uncharacterized protein LOC101207943 [Cucumis sativus] >KGN50301.1 hypothetical protein Csa_000149 [Cucumis sativus])

HSP 1 Score: 926 bits (2392), Expect = 0.0
Identity = 449/449 (100.00%), Postives = 449/449 (100.00%), Query Frame = 0

Query: 1   MSILCGVPILECVCCLGCARWVWKRCLHTAGHDSENWGFATPDEFEPIPRICRYILSVYE 60
           MSILCGVPILECVCCLGCARWVWKRCLHTAGHDSENWGFATPDEFEPIPRICRYILSVYE
Sbjct: 1   MSILCGVPILECVCCLGCARWVWKRCLHTAGHDSENWGFATPDEFEPIPRICRYILSVYE 60

Query: 61  DDIRKPLWEPVGGYGINPDWLLMKKTYKDTRGRAPPYILYLDHVHADIVLAIRGLNMAKE 120
           DDIRKPLWEPVGGYGINPDWLLMKKTYKDTRGRAPPYILYLDHVHADIVLAIRGLNMAKE
Sbjct: 61  DDIRKPLWEPVGGYGINPDWLLMKKTYKDTRGRAPPYILYLDHVHADIVLAIRGLNMAKE 120

Query: 121 SDYAVLLDNRLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLG 180
           SDYAVLLDNRLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLG
Sbjct: 121 SDYAVLLDNRLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLG 180

Query: 181 SGVAAMLTLLVVQNREKLENIDRKRIRCFAIAPARCMSLNLAVRYADVINSVVLQDDFLP 240
           SGVAAMLTLLVVQNREKLENIDRKRIRCFAIAPARCMSLNLAVRYADVINSVVLQDDFLP
Sbjct: 181 SGVAAMLTLLVVQNREKLENIDRKRIRCFAIAPARCMSLNLAVRYADVINSVVLQDDFLP 240

Query: 241 RTATPLEDIFKSLFCLPCLLCLRCVRDTCVSEEKMLKDPRRLYAPGRLYHIVERKPFRCG 300
           RTATPLEDIFKSLFCLPCLLCLRCVRDTCVSEEKMLKDPRRLYAPGRLYHIVERKPFRCG
Sbjct: 241 RTATPLEDIFKSLFCLPCLLCLRCVRDTCVSEEKMLKDPRRLYAPGRLYHIVERKPFRCG 300

Query: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMRKDDKIMEIPPQQKM 360
           RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMRKDDKIMEIPPQQKM
Sbjct: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMRKDDKIMEIPPQQKM 360

Query: 361 ERQQTLAREHSEEYKAALQRAVTLAVPHAYAISPYGTFSQTDEGEEEKSPASSGGSSRRR 420
           ERQQTLAREHSEEYKAALQRAVTLAVPHAYAISPYGTFSQTDEGEEEKSPASSGGSSRRR
Sbjct: 361 ERQQTLAREHSEEYKAALQRAVTLAVPHAYAISPYGTFSQTDEGEEEKSPASSGGSSRRR 420

Query: 421 KETWDELIERLYDKDDSRHTVLKKSLSCI 449
           KETWDELIERLYDKDDSRHTVLKKSLSCI
Sbjct: 421 KETWDELIERLYDKDDSRHTVLKKSLSCI 449

BLAST of Cucsat.G21422 vs. NCBI nr
Match: XP_008436992.1 (PREDICTED: uncharacterized protein LOC103482554 [Cucumis melo] >XP_008436993.1 PREDICTED: uncharacterized protein LOC103482554 [Cucumis melo] >KAA0043467.1 Alpha/beta-Hydrolases superfamily protein isoform 1 [Cucumis melo var. makuwa])

HSP 1 Score: 911 bits (2355), Expect = 0.0
Identity = 442/449 (98.44%), Postives = 445/449 (99.11%), Query Frame = 0

Query: 1   MSILCGVPILECVCCLGCARWVWKRCLHTAGHDSENWGFATPDEFEPIPRICRYILSVYE 60
           MSILCGVPILECVCCLGCARWVWKRCLHTAGHDSENWGFATPDEFEPIPRICRYILSVYE
Sbjct: 1   MSILCGVPILECVCCLGCARWVWKRCLHTAGHDSENWGFATPDEFEPIPRICRYILSVYE 60

Query: 61  DDIRKPLWEPVGGYGINPDWLLMKKTYKDTRGRAPPYILYLDHVHADIVLAIRGLNMAKE 120
           DDIRKPLWEPVGGYGINPDWL+MKKTYKDTRGRAPPYILYLDH HADIVLAIRGLNM KE
Sbjct: 61  DDIRKPLWEPVGGYGINPDWLIMKKTYKDTRGRAPPYILYLDHDHADIVLAIRGLNMVKE 120

Query: 121 SDYAVLLDNRLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLG 180
           SDYAVLLDNRLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTF GHSLG
Sbjct: 121 SDYAVLLDNRLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFTGHSLG 180

Query: 181 SGVAAMLTLLVVQNREKLENIDRKRIRCFAIAPARCMSLNLAVRYADVINSVVLQDDFLP 240
           SGVAAMLTL+VVQNREKLENIDRKRIRC+AIAPARCMSLNLAVRYADVINSVVLQDDFLP
Sbjct: 181 SGVAAMLTLVVVQNREKLENIDRKRIRCYAIAPARCMSLNLAVRYADVINSVVLQDDFLP 240

Query: 241 RTATPLEDIFKSLFCLPCLLCLRCVRDTCVSEEKMLKDPRRLYAPGRLYHIVERKPFRCG 300
           RTATPLEDIFKSLFCLPCLLCLRCVRDTCVSEEKMLKDPRRLYAPGRLYHIVERKPFRCG
Sbjct: 241 RTATPLEDIFKSLFCLPCLLCLRCVRDTCVSEEKMLKDPRRLYAPGRLYHIVERKPFRCG 300

Query: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMRKDDKIMEIPPQQKM 360
           RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAK ALELMRKDDKIMEIPPQQKM
Sbjct: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKRALELMRKDDKIMEIPPQQKM 360

Query: 361 ERQQTLAREHSEEYKAALQRAVTLAVPHAYAISPYGTFSQTDEGEEEKSPASSGGSSRRR 420
           ERQQTLAREHSEEYKAALQRAVTLAVPHAYAISPYGTFSQTDEGEEEKSPASSGGSSRRR
Sbjct: 361 ERQQTLAREHSEEYKAALQRAVTLAVPHAYAISPYGTFSQTDEGEEEKSPASSGGSSRRR 420

Query: 421 KETWDELIERLYDKDDSRHTVLKKSLSCI 449
           KETWDELIERLYDKDDSRHTVLKKSLSCI
Sbjct: 421 KETWDELIERLYDKDDSRHTVLKKSLSCI 449

BLAST of Cucsat.G21422 vs. NCBI nr
Match: XP_038876038.1 (uncharacterized protein LOC120068369 [Benincasa hispida] >XP_038876039.1 uncharacterized protein LOC120068369 [Benincasa hispida])

HSP 1 Score: 885 bits (2286), Expect = 0.0
Identity = 428/449 (95.32%), Postives = 439/449 (97.77%), Query Frame = 0

Query: 1   MSILCGVPILECVCCLGCARWVWKRCLHTAGHDSENWGFATPDEFEPIPRICRYILSVYE 60
           MSILCGVPILECVCCLGCARWVWKRCLHTAGHDSE WGFATPDEFEPIPRICRYILSVYE
Sbjct: 1   MSILCGVPILECVCCLGCARWVWKRCLHTAGHDSETWGFATPDEFEPIPRICRYILSVYE 60

Query: 61  DDIRKPLWEPVGGYGINPDWLLMKKTYKDTRGRAPPYILYLDHVHADIVLAIRGLNMAKE 120
           DDIRKPLWEPVGGYGINPDWLLMKKTYKDTRGRAPPYILYLDH HADIVLAIRGLNMAKE
Sbjct: 61  DDIRKPLWEPVGGYGINPDWLLMKKTYKDTRGRAPPYILYLDHNHADIVLAIRGLNMAKE 120

Query: 121 SDYAVLLDNRLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLG 180
           SDYAVLLDNRLGKKKFDGGYVHNGLLKAAGWVLD ENETLKDLVKKYPDYTLTFAGHSLG
Sbjct: 121 SDYAVLLDNRLGKKKFDGGYVHNGLLKAAGWVLDNENETLKDLVKKYPDYTLTFAGHSLG 180

Query: 181 SGVAAMLTLLVVQNREKLENIDRKRIRCFAIAPARCMSLNLAVRYADVINSVVLQDDFLP 240
           SGVAAMLTL+VVQN +KLENIDRKRIRC+AIAPARCMSLNLAVRYADVINSVVLQDDFLP
Sbjct: 181 SGVAAMLTLVVVQNHKKLENIDRKRIRCYAIAPARCMSLNLAVRYADVINSVVLQDDFLP 240

Query: 241 RTATPLEDIFKSLFCLPCLLCLRCVRDTCVSEEKMLKDPRRLYAPGRLYHIVERKPFRCG 300
           RTATPLEDIFKSLFCLPCLLCLRCVRDTC+SE+KMLKDPRRLYAPGRLYHIVERKPFRCG
Sbjct: 241 RTATPLEDIFKSLFCLPCLLCLRCVRDTCISEDKMLKDPRRLYAPGRLYHIVERKPFRCG 300

Query: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMRKDDKIMEIPPQQKM 360
           RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAK ALELMRK DK+MEIPPQQKM
Sbjct: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKRALELMRKADKVMEIPPQQKM 360

Query: 361 ERQQTLAREHSEEYKAALQRAVTLAVPHAYAISPYGTFSQTDEGEEEKSPASSGGSSRRR 420
           ERQ+TLAREHSEEYKAALQRAVTLAVPHAYA SPYGTFSQT+EGEE++SPASSGGS RR+
Sbjct: 361 ERQKTLAREHSEEYKAALQRAVTLAVPHAYAFSPYGTFSQTEEGEEKESPASSGGSLRRK 420

Query: 421 KETWDELIERLYDKDDSRHTVLKKSLSCI 449
           KETWDELIERLYDKDDSRHTVLKKS S I
Sbjct: 421 KETWDELIERLYDKDDSRHTVLKKSHSSI 449

BLAST of Cucsat.G21422 vs. NCBI nr
Match: XP_023550858.1 (uncharacterized protein LOC111808873 [Cucurbita pepo subsp. pepo] >XP_023550859.1 uncharacterized protein LOC111808873 [Cucurbita pepo subsp. pepo] >XP_023550860.1 uncharacterized protein LOC111808873 [Cucurbita pepo subsp. pepo] >XP_023550861.1 uncharacterized protein LOC111808873 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 861 bits (2225), Expect = 1.67e-314
Identity = 417/459 (90.85%), Postives = 437/459 (95.21%), Query Frame = 0

Query: 1   MSILCGVPILECVCCLGCARWVWKRCLHTAGHDSENWGFATPDEFEPIPRICRYILSVYE 60
           MSI+CGVPILECVCCLGCARWVWKRCLHTAGHDSE WGFAT DEFEPIPRICRYIL+VYE
Sbjct: 1   MSIICGVPILECVCCLGCARWVWKRCLHTAGHDSETWGFATADEFEPIPRICRYILAVYE 60

Query: 61  DDIRKPLWEPVGGYGINPDWLLMKKTYKDTRGRAPPYILYLDHVHADIVLAIRGLNMAKE 120
           DDIRKPLWEPVGGYGINPDWL++KKTYKDTRGRAPPYILYLDH HADIVLAIRGLN+AKE
Sbjct: 61  DDIRKPLWEPVGGYGINPDWLILKKTYKDTRGRAPPYILYLDHDHADIVLAIRGLNLAKE 120

Query: 121 SDYAVLLDNRLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLG 180
           SDYAVLLDN+LGK KFDGGYVHNGLLKAAGWVLD+ENETLKDLVKKYPDYTLTFAGHSLG
Sbjct: 121 SDYAVLLDNKLGKMKFDGGYVHNGLLKAAGWVLDSENETLKDLVKKYPDYTLTFAGHSLG 180

Query: 181 SGVAAMLTLLVVQNREKLENIDRKRIRCFAIAPARCMSLNLAVRYADVINSVVLQDDFLP 240
           SGVAAMLTL+VVQN EKLENIDRKRIRC+AIAPARCMSLNLAVRYADVINSVVLQDDFLP
Sbjct: 181 SGVAAMLTLVVVQNHEKLENIDRKRIRCYAIAPARCMSLNLAVRYADVINSVVLQDDFLP 240

Query: 241 RTATPLEDIFKSLFCLPCLLCLRCVRDTCVSEEKMLKDPRRLYAPGRLYHIVERKPFRCG 300
           RTATPLEDIFKSLFCLPCLLCL C+RDTC+SE+KMLKDPRRLY PGRLYHIVERKPFRCG
Sbjct: 241 RTATPLEDIFKSLFCLPCLLCLGCLRDTCISEDKMLKDPRRLYTPGRLYHIVERKPFRCG 300

Query: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMRKDDKIMEIPPQQKM 360
           RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELM +D+K+MEIPPQQKM
Sbjct: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMLEDNKVMEIPPQQKM 360

Query: 361 ERQQTLAREHSEEYKAALQRAVTLAVPHAYAISPYGTFSQTDEGEEEK---SPASSGGSS 420
           ERQ TLAREH+EEYKAALQRAVTL+VPHAYA+SPYGTFS+T EGEEEK   SPASSGGSS
Sbjct: 361 ERQNTLAREHTEEYKAALQRAVTLSVPHAYALSPYGTFSETVEGEEEKEEESPASSGGSS 420

Query: 421 RRRKETWDELIERLYDKDDSRH-------TVLKKSLSCI 449
           R+RKETWDELIERLYDKDDSRH       TVLKKS S I
Sbjct: 421 RKRKETWDELIERLYDKDDSRHNKNESRYTVLKKSFSSI 459

BLAST of Cucsat.G21422 vs. NCBI nr
Match: XP_022922227.1 (uncharacterized protein LOC111430275 [Cucurbita moschata] >XP_022922228.1 uncharacterized protein LOC111430275 [Cucurbita moschata] >XP_022922229.1 uncharacterized protein LOC111430275 [Cucurbita moschata] >XP_022922230.1 uncharacterized protein LOC111430275 [Cucurbita moschata])

HSP 1 Score: 861 bits (2224), Expect = 2.34e-314
Identity = 417/459 (90.85%), Postives = 436/459 (94.99%), Query Frame = 0

Query: 1   MSILCGVPILECVCCLGCARWVWKRCLHTAGHDSENWGFATPDEFEPIPRICRYILSVYE 60
           MSI+CGVPILECVCCLGCARWVWKRCLHTAGHDSE WGFAT DEFEPIPRICRYIL+VYE
Sbjct: 1   MSIICGVPILECVCCLGCARWVWKRCLHTAGHDSETWGFATADEFEPIPRICRYILAVYE 60

Query: 61  DDIRKPLWEPVGGYGINPDWLLMKKTYKDTRGRAPPYILYLDHVHADIVLAIRGLNMAKE 120
           DDIRKPLWEPVGGYGINPDWL++KKTYKDTRGRAPPYILYLDH HADIVLAIRGLN+AKE
Sbjct: 61  DDIRKPLWEPVGGYGINPDWLILKKTYKDTRGRAPPYILYLDHDHADIVLAIRGLNLAKE 120

Query: 121 SDYAVLLDNRLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLG 180
           SDYAVLLDN+LGK KFDGGYVHNGLLKAAGWVLD+ENETLKDLVKKYPDYTLTFAGHSLG
Sbjct: 121 SDYAVLLDNKLGKMKFDGGYVHNGLLKAAGWVLDSENETLKDLVKKYPDYTLTFAGHSLG 180

Query: 181 SGVAAMLTLLVVQNREKLENIDRKRIRCFAIAPARCMSLNLAVRYADVINSVVLQDDFLP 240
           SGVAAMLTL+VVQN EKLENIDRKRIRC+AIAPARCMSLNLAVRYADVINSVVLQDDFLP
Sbjct: 181 SGVAAMLTLVVVQNHEKLENIDRKRIRCYAIAPARCMSLNLAVRYADVINSVVLQDDFLP 240

Query: 241 RTATPLEDIFKSLFCLPCLLCLRCVRDTCVSEEKMLKDPRRLYAPGRLYHIVERKPFRCG 300
           RTATPLEDIFKSLFCLPCLLCL C+RDTC+SE+KMLKDPRRLY PGRLYHIVERKPFRCG
Sbjct: 241 RTATPLEDIFKSLFCLPCLLCLGCLRDTCISEDKMLKDPRRLYTPGRLYHIVERKPFRCG 300

Query: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMRKDDKIMEIPPQQKM 360
           RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELM +D+K+MEIPPQQKM
Sbjct: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMLEDNKVMEIPPQQKM 360

Query: 361 ERQQTLAREHSEEYKAALQRAVTLAVPHAYAISPYGTFSQTDEGEEEK---SPASSGGSS 420
           ERQ TLAREH+EEYKAALQRAVTLAVPHAY +SPYGTFS+T EGEEEK   SPASSGGSS
Sbjct: 361 ERQNTLAREHTEEYKAALQRAVTLAVPHAYTLSPYGTFSETVEGEEEKEEESPASSGGSS 420

Query: 421 RRRKETWDELIERLYDKDDSRH-------TVLKKSLSCI 449
           R+RKETWDELIERLYDKDDSRH       TVLKKS S I
Sbjct: 421 RKRKETWDELIERLYDKDDSRHDKNESRYTVLKKSFSSI 459

BLAST of Cucsat.G21422 vs. ExPASy TrEMBL
Match: A0A0A0KRF0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G166450 PE=4 SV=1)

HSP 1 Score: 926 bits (2392), Expect = 0.0
Identity = 449/449 (100.00%), Postives = 449/449 (100.00%), Query Frame = 0

Query: 1   MSILCGVPILECVCCLGCARWVWKRCLHTAGHDSENWGFATPDEFEPIPRICRYILSVYE 60
           MSILCGVPILECVCCLGCARWVWKRCLHTAGHDSENWGFATPDEFEPIPRICRYILSVYE
Sbjct: 1   MSILCGVPILECVCCLGCARWVWKRCLHTAGHDSENWGFATPDEFEPIPRICRYILSVYE 60

Query: 61  DDIRKPLWEPVGGYGINPDWLLMKKTYKDTRGRAPPYILYLDHVHADIVLAIRGLNMAKE 120
           DDIRKPLWEPVGGYGINPDWLLMKKTYKDTRGRAPPYILYLDHVHADIVLAIRGLNMAKE
Sbjct: 61  DDIRKPLWEPVGGYGINPDWLLMKKTYKDTRGRAPPYILYLDHVHADIVLAIRGLNMAKE 120

Query: 121 SDYAVLLDNRLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLG 180
           SDYAVLLDNRLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLG
Sbjct: 121 SDYAVLLDNRLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLG 180

Query: 181 SGVAAMLTLLVVQNREKLENIDRKRIRCFAIAPARCMSLNLAVRYADVINSVVLQDDFLP 240
           SGVAAMLTLLVVQNREKLENIDRKRIRCFAIAPARCMSLNLAVRYADVINSVVLQDDFLP
Sbjct: 181 SGVAAMLTLLVVQNREKLENIDRKRIRCFAIAPARCMSLNLAVRYADVINSVVLQDDFLP 240

Query: 241 RTATPLEDIFKSLFCLPCLLCLRCVRDTCVSEEKMLKDPRRLYAPGRLYHIVERKPFRCG 300
           RTATPLEDIFKSLFCLPCLLCLRCVRDTCVSEEKMLKDPRRLYAPGRLYHIVERKPFRCG
Sbjct: 241 RTATPLEDIFKSLFCLPCLLCLRCVRDTCVSEEKMLKDPRRLYAPGRLYHIVERKPFRCG 300

Query: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMRKDDKIMEIPPQQKM 360
           RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMRKDDKIMEIPPQQKM
Sbjct: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMRKDDKIMEIPPQQKM 360

Query: 361 ERQQTLAREHSEEYKAALQRAVTLAVPHAYAISPYGTFSQTDEGEEEKSPASSGGSSRRR 420
           ERQQTLAREHSEEYKAALQRAVTLAVPHAYAISPYGTFSQTDEGEEEKSPASSGGSSRRR
Sbjct: 361 ERQQTLAREHSEEYKAALQRAVTLAVPHAYAISPYGTFSQTDEGEEEKSPASSGGSSRRR 420

Query: 421 KETWDELIERLYDKDDSRHTVLKKSLSCI 449
           KETWDELIERLYDKDDSRHTVLKKSLSCI
Sbjct: 421 KETWDELIERLYDKDDSRHTVLKKSLSCI 449

BLAST of Cucsat.G21422 vs. ExPASy TrEMBL
Match: A0A1S3ASL8 (uncharacterized protein LOC103482554 OS=Cucumis melo OX=3656 GN=LOC103482554 PE=4 SV=1)

HSP 1 Score: 911 bits (2355), Expect = 0.0
Identity = 442/449 (98.44%), Postives = 445/449 (99.11%), Query Frame = 0

Query: 1   MSILCGVPILECVCCLGCARWVWKRCLHTAGHDSENWGFATPDEFEPIPRICRYILSVYE 60
           MSILCGVPILECVCCLGCARWVWKRCLHTAGHDSENWGFATPDEFEPIPRICRYILSVYE
Sbjct: 1   MSILCGVPILECVCCLGCARWVWKRCLHTAGHDSENWGFATPDEFEPIPRICRYILSVYE 60

Query: 61  DDIRKPLWEPVGGYGINPDWLLMKKTYKDTRGRAPPYILYLDHVHADIVLAIRGLNMAKE 120
           DDIRKPLWEPVGGYGINPDWL+MKKTYKDTRGRAPPYILYLDH HADIVLAIRGLNM KE
Sbjct: 61  DDIRKPLWEPVGGYGINPDWLIMKKTYKDTRGRAPPYILYLDHDHADIVLAIRGLNMVKE 120

Query: 121 SDYAVLLDNRLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLG 180
           SDYAVLLDNRLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTF GHSLG
Sbjct: 121 SDYAVLLDNRLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFTGHSLG 180

Query: 181 SGVAAMLTLLVVQNREKLENIDRKRIRCFAIAPARCMSLNLAVRYADVINSVVLQDDFLP 240
           SGVAAMLTL+VVQNREKLENIDRKRIRC+AIAPARCMSLNLAVRYADVINSVVLQDDFLP
Sbjct: 181 SGVAAMLTLVVVQNREKLENIDRKRIRCYAIAPARCMSLNLAVRYADVINSVVLQDDFLP 240

Query: 241 RTATPLEDIFKSLFCLPCLLCLRCVRDTCVSEEKMLKDPRRLYAPGRLYHIVERKPFRCG 300
           RTATPLEDIFKSLFCLPCLLCLRCVRDTCVSEEKMLKDPRRLYAPGRLYHIVERKPFRCG
Sbjct: 241 RTATPLEDIFKSLFCLPCLLCLRCVRDTCVSEEKMLKDPRRLYAPGRLYHIVERKPFRCG 300

Query: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMRKDDKIMEIPPQQKM 360
           RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAK ALELMRKDDKIMEIPPQQKM
Sbjct: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKRALELMRKDDKIMEIPPQQKM 360

Query: 361 ERQQTLAREHSEEYKAALQRAVTLAVPHAYAISPYGTFSQTDEGEEEKSPASSGGSSRRR 420
           ERQQTLAREHSEEYKAALQRAVTLAVPHAYAISPYGTFSQTDEGEEEKSPASSGGSSRRR
Sbjct: 361 ERQQTLAREHSEEYKAALQRAVTLAVPHAYAISPYGTFSQTDEGEEEKSPASSGGSSRRR 420

Query: 421 KETWDELIERLYDKDDSRHTVLKKSLSCI 449
           KETWDELIERLYDKDDSRHTVLKKSLSCI
Sbjct: 421 KETWDELIERLYDKDDSRHTVLKKSLSCI 449

BLAST of Cucsat.G21422 vs. ExPASy TrEMBL
Match: A0A5A7TNW3 (Alpha/beta-Hydrolases superfamily protein isoform 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1167G00050 PE=4 SV=1)

HSP 1 Score: 911 bits (2355), Expect = 0.0
Identity = 442/449 (98.44%), Postives = 445/449 (99.11%), Query Frame = 0

Query: 1   MSILCGVPILECVCCLGCARWVWKRCLHTAGHDSENWGFATPDEFEPIPRICRYILSVYE 60
           MSILCGVPILECVCCLGCARWVWKRCLHTAGHDSENWGFATPDEFEPIPRICRYILSVYE
Sbjct: 1   MSILCGVPILECVCCLGCARWVWKRCLHTAGHDSENWGFATPDEFEPIPRICRYILSVYE 60

Query: 61  DDIRKPLWEPVGGYGINPDWLLMKKTYKDTRGRAPPYILYLDHVHADIVLAIRGLNMAKE 120
           DDIRKPLWEPVGGYGINPDWL+MKKTYKDTRGRAPPYILYLDH HADIVLAIRGLNM KE
Sbjct: 61  DDIRKPLWEPVGGYGINPDWLIMKKTYKDTRGRAPPYILYLDHDHADIVLAIRGLNMVKE 120

Query: 121 SDYAVLLDNRLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLG 180
           SDYAVLLDNRLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTF GHSLG
Sbjct: 121 SDYAVLLDNRLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFTGHSLG 180

Query: 181 SGVAAMLTLLVVQNREKLENIDRKRIRCFAIAPARCMSLNLAVRYADVINSVVLQDDFLP 240
           SGVAAMLTL+VVQNREKLENIDRKRIRC+AIAPARCMSLNLAVRYADVINSVVLQDDFLP
Sbjct: 181 SGVAAMLTLVVVQNREKLENIDRKRIRCYAIAPARCMSLNLAVRYADVINSVVLQDDFLP 240

Query: 241 RTATPLEDIFKSLFCLPCLLCLRCVRDTCVSEEKMLKDPRRLYAPGRLYHIVERKPFRCG 300
           RTATPLEDIFKSLFCLPCLLCLRCVRDTCVSEEKMLKDPRRLYAPGRLYHIVERKPFRCG
Sbjct: 241 RTATPLEDIFKSLFCLPCLLCLRCVRDTCVSEEKMLKDPRRLYAPGRLYHIVERKPFRCG 300

Query: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMRKDDKIMEIPPQQKM 360
           RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAK ALELMRKDDKIMEIPPQQKM
Sbjct: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKRALELMRKDDKIMEIPPQQKM 360

Query: 361 ERQQTLAREHSEEYKAALQRAVTLAVPHAYAISPYGTFSQTDEGEEEKSPASSGGSSRRR 420
           ERQQTLAREHSEEYKAALQRAVTLAVPHAYAISPYGTFSQTDEGEEEKSPASSGGSSRRR
Sbjct: 361 ERQQTLAREHSEEYKAALQRAVTLAVPHAYAISPYGTFSQTDEGEEEKSPASSGGSSRRR 420

Query: 421 KETWDELIERLYDKDDSRHTVLKKSLSCI 449
           KETWDELIERLYDKDDSRHTVLKKSLSCI
Sbjct: 421 KETWDELIERLYDKDDSRHTVLKKSLSCI 449

BLAST of Cucsat.G21422 vs. ExPASy TrEMBL
Match: A0A6J1E2T3 (uncharacterized protein LOC111430275 OS=Cucurbita moschata OX=3662 GN=LOC111430275 PE=4 SV=1)

HSP 1 Score: 861 bits (2224), Expect = 1.13e-314
Identity = 417/459 (90.85%), Postives = 436/459 (94.99%), Query Frame = 0

Query: 1   MSILCGVPILECVCCLGCARWVWKRCLHTAGHDSENWGFATPDEFEPIPRICRYILSVYE 60
           MSI+CGVPILECVCCLGCARWVWKRCLHTAGHDSE WGFAT DEFEPIPRICRYIL+VYE
Sbjct: 1   MSIICGVPILECVCCLGCARWVWKRCLHTAGHDSETWGFATADEFEPIPRICRYILAVYE 60

Query: 61  DDIRKPLWEPVGGYGINPDWLLMKKTYKDTRGRAPPYILYLDHVHADIVLAIRGLNMAKE 120
           DDIRKPLWEPVGGYGINPDWL++KKTYKDTRGRAPPYILYLDH HADIVLAIRGLN+AKE
Sbjct: 61  DDIRKPLWEPVGGYGINPDWLILKKTYKDTRGRAPPYILYLDHDHADIVLAIRGLNLAKE 120

Query: 121 SDYAVLLDNRLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLG 180
           SDYAVLLDN+LGK KFDGGYVHNGLLKAAGWVLD+ENETLKDLVKKYPDYTLTFAGHSLG
Sbjct: 121 SDYAVLLDNKLGKMKFDGGYVHNGLLKAAGWVLDSENETLKDLVKKYPDYTLTFAGHSLG 180

Query: 181 SGVAAMLTLLVVQNREKLENIDRKRIRCFAIAPARCMSLNLAVRYADVINSVVLQDDFLP 240
           SGVAAMLTL+VVQN EKLENIDRKRIRC+AIAPARCMSLNLAVRYADVINSVVLQDDFLP
Sbjct: 181 SGVAAMLTLVVVQNHEKLENIDRKRIRCYAIAPARCMSLNLAVRYADVINSVVLQDDFLP 240

Query: 241 RTATPLEDIFKSLFCLPCLLCLRCVRDTCVSEEKMLKDPRRLYAPGRLYHIVERKPFRCG 300
           RTATPLEDIFKSLFCLPCLLCL C+RDTC+SE+KMLKDPRRLY PGRLYHIVERKPFRCG
Sbjct: 241 RTATPLEDIFKSLFCLPCLLCLGCLRDTCISEDKMLKDPRRLYTPGRLYHIVERKPFRCG 300

Query: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMRKDDKIMEIPPQQKM 360
           RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELM +D+K+MEIPPQQKM
Sbjct: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMLEDNKVMEIPPQQKM 360

Query: 361 ERQQTLAREHSEEYKAALQRAVTLAVPHAYAISPYGTFSQTDEGEEEK---SPASSGGSS 420
           ERQ TLAREH+EEYKAALQRAVTLAVPHAY +SPYGTFS+T EGEEEK   SPASSGGSS
Sbjct: 361 ERQNTLAREHTEEYKAALQRAVTLAVPHAYTLSPYGTFSETVEGEEEKEEESPASSGGSS 420

Query: 421 RRRKETWDELIERLYDKDDSRH-------TVLKKSLSCI 449
           R+RKETWDELIERLYDKDDSRH       TVLKKS S I
Sbjct: 421 RKRKETWDELIERLYDKDDSRHDKNESRYTVLKKSFSSI 459

BLAST of Cucsat.G21422 vs. ExPASy TrEMBL
Match: A0A6J1I6B2 (uncharacterized protein LOC111471517 OS=Cucurbita maxima OX=3661 GN=LOC111471517 PE=4 SV=1)

HSP 1 Score: 853 bits (2205), Expect = 8.67e-312
Identity = 414/459 (90.20%), Postives = 433/459 (94.34%), Query Frame = 0

Query: 1   MSILCGVPILECVCCLGCARWVWKRCLHTAGHDSENWGFATPDEFEPIPRICRYILSVYE 60
           MSI+CGVPILECVCCLGCARWVWKRCLHTAGHDSE WGFAT DEFEPIPRICRYIL+VYE
Sbjct: 1   MSIICGVPILECVCCLGCARWVWKRCLHTAGHDSETWGFATTDEFEPIPRICRYILAVYE 60

Query: 61  DDIRKPLWEPVGGYGINPDWLLMKKTYKDTRGRAPPYILYLDHVHADIVLAIRGLNMAKE 120
           DDIRKPLWEPVGGYGINPDWLL+KKTYKDTRGRAPPYILYLDH H DIVLAIRGLN+AKE
Sbjct: 61  DDIRKPLWEPVGGYGINPDWLLLKKTYKDTRGRAPPYILYLDHDHGDIVLAIRGLNLAKE 120

Query: 121 SDYAVLLDNRLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLG 180
           SDYAVLLDN+LGK KFDGGYVHNGLLKAAGWVLD+ENETLKDLVKKYPDYTLTFAGHSLG
Sbjct: 121 SDYAVLLDNKLGKMKFDGGYVHNGLLKAAGWVLDSENETLKDLVKKYPDYTLTFAGHSLG 180

Query: 181 SGVAAMLTLLVVQNREKLENIDRKRIRCFAIAPARCMSLNLAVRYADVINSVVLQDDFLP 240
           SGVAAMLTL+VVQN +KLENIDRKRIRC+AIAPARCMSLNLAVRYADVINSVVLQDDFLP
Sbjct: 181 SGVAAMLTLVVVQNHKKLENIDRKRIRCYAIAPARCMSLNLAVRYADVINSVVLQDDFLP 240

Query: 241 RTATPLEDIFKSLFCLPCLLCLRCVRDTCVSEEKMLKDPRRLYAPGRLYHIVERKPFRCG 300
           RTATPLEDIFKSLFCLPCLLCL C+RDTC+SE+KMLKDPRRLY PGRLYHIVERKPFRCG
Sbjct: 241 RTATPLEDIFKSLFCLPCLLCLGCLRDTCISEDKMLKDPRRLYTPGRLYHIVERKPFRCG 300

Query: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMRKDDKIMEIPPQQKM 360
           RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELM +D+K+M IPPQQKM
Sbjct: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMLEDNKVMGIPPQQKM 360

Query: 361 ERQQTLAREHSEEYKAALQRAVTLAVPHAYAISPYGTFSQTDEGEEEK---SPASSGGSS 420
           ERQ TLAREH+EEYKAALQRAVTLAVPHAY +SPYGTFS+T EGEEEK   SPASSGGS 
Sbjct: 361 ERQNTLAREHTEEYKAALQRAVTLAVPHAYTLSPYGTFSETVEGEEEKEEESPASSGGSL 420

Query: 421 RRRKETWDELIERLYDKDDSRH-------TVLKKSLSCI 449
           R+RKETWDELIERLYDKDDSRH       TVLKKS S I
Sbjct: 421 RKRKETWDELIERLYDKDDSRHDKNESRYTVLKKSFSSI 459

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8NCG72.2e-0728.66Diacylglycerol lipase-beta OS=Homo sapiens OX=9606 GN=DAGLB PE=1 SV=2[more]
Q91WC96.4e-0727.44Diacylglycerol lipase-beta OS=Mus musculus OX=10090 GN=Daglb PE=1 SV=2[more]
P0C1S98.3e-0726.25Diacylglycerol lipase-beta OS=Rattus norvegicus OX=10116 GN=Daglb PE=1 SV=1[more]
Q5YLM11.2e-0524.38Diacylglycerol lipase-alpha OS=Rattus norvegicus OX=10116 GN=Dagla PE=1 SV=1[more]
Q6WQJ11.6e-0524.38Diacylglycerol lipase-alpha OS=Mus musculus OX=10090 GN=Dagla PE=1 SV=2[more]
Match NameE-valueIdentityDescription
XP_004152416.10.0100.00uncharacterized protein LOC101207943 [Cucumis sativus] >XP_011654833.1 uncharact... [more]
XP_008436992.10.098.44PREDICTED: uncharacterized protein LOC103482554 [Cucumis melo] >XP_008436993.1 P... [more]
XP_038876038.10.095.32uncharacterized protein LOC120068369 [Benincasa hispida] >XP_038876039.1 unchara... [more]
XP_023550858.11.67e-31490.85uncharacterized protein LOC111808873 [Cucurbita pepo subsp. pepo] >XP_023550859.... [more]
XP_022922227.12.34e-31490.85uncharacterized protein LOC111430275 [Cucurbita moschata] >XP_022922228.1 unchar... [more]
Match NameE-valueIdentityDescription
A0A0A0KRF00.0100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G166450 PE=4 SV=1[more]
A0A1S3ASL80.098.44uncharacterized protein LOC103482554 OS=Cucumis melo OX=3656 GN=LOC103482554 PE=... [more]
A0A5A7TNW30.098.44Alpha/beta-Hydrolases superfamily protein isoform 1 OS=Cucumis melo var. makuwa ... [more]
A0A6J1E2T31.13e-31490.85uncharacterized protein LOC111430275 OS=Cucurbita moschata OX=3662 GN=LOC1114302... [more]
A0A6J1I6B28.67e-31290.20uncharacterized protein LOC111471517 OS=Cucurbita maxima OX=3661 GN=LOC111471517... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005592Mono-/di-acylglycerol lipase, N-terminalPFAMPF03893Lipase3_Ncoord: 10..73
e-value: 1.1E-19
score: 70.2
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 42..252
e-value: 7.6E-26
score: 92.8
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 41..271
IPR002921Fungal lipase-like domainPFAMPF01764Lipase_3coord: 109..243
e-value: 1.2E-23
score: 83.6
NoneNo IPR availablePANTHERPTHR46398:SF4ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 1..344
NoneNo IPR availablePANTHERPTHR46398ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 1..344
NoneNo IPR availableCDDcd00519Lipase_3coord: 44..241
e-value: 4.88474E-28
score: 107.947

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G21422.T1Cucsat.G21422.T1mRNA
Cucsat.G21422.T2Cucsat.G21422.T2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016042 lipid catabolic process
biological_process GO:0006629 lipid metabolic process
molecular_function GO:0016787 hydrolase activity