Cucsat.G2035 (gene) Cucumber (B10) v3

BLAST of Cucsat.G2035 vs. ExPASy Swiss-Prot
Match: Q6NMR9 (GDSL esterase/lipase At5g45920 OS=Arabidopsis thaliana OX=3702 GN=At5g45920 PE=2 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 1.7e-43
Identity = 77/131 (58.78%), Postives = 101/131 (77.10%), Query Frame = 0

Query: 11  QKKWPATRILLITPPPIDEEGRLQNPYVSNPVNEPERTNDAAGAYAKACIAVAKECGVSV 70
           + +WP T I+LITPPPIDEE RL+ PY+ N    PERTN+ AG YAKACIAVA+EC +SV
Sbjct: 110 KNRWPQTAIILITPPPIDEEARLRYPYIENTTGLPERTNEVAGLYAKACIAVAEECQISV 169

Query: 71  IDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPVTLPADLPLISEI 130
            D+W+KMQQ+P W+  CL DGLHL+R GN +VFEEV K+L+EEG+    L  DLPLI ++
Sbjct: 170 TDLWSKMQQIPNWQTECLWDGLHLSRVGNKVVFEEVAKKLKEEGIGAEDLAVDLPLIEDV 229

Query: 131 DFNNPLQAFEK 142
           D  +PL++F++
Sbjct: 230 DPKDPLKSFDE 240

BLAST of Cucsat.G2035 vs. ExPASy Swiss-Prot
Match: Q9FM04 (GDSL esterase/lipase At5g62930 OS=Arabidopsis thaliana OX=3702 GN=At5g62930 PE=2 SV=3)

HSP 1 Score: 130.6 bits (327), Expect = 1.4e-29
Identity = 63/130 (48.46%), Postives = 85/130 (65.38%), Query Frame = 0

Query: 15  PATRILLITPPPIDEEGR---LQNPYVSNPVNEPERTNDAAGAYAKACIAVAKECGVSVI 74
           P   I+LITPPPIDE GR    ++ Y    + EPERTN+  G YA+ C+A+A+E G+  +
Sbjct: 110 PTMLIVLITPPPIDEAGRQSYAESIYGEKAMKEPERTNETTGVYAQHCVALAEELGLRCV 169

Query: 75  DIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPVTLPADLPLISEID 134
           ++W+KMQ+   W+K  LSDGLHLT  GN +VF+EV +   E  LSP  +P D P  S ID
Sbjct: 170 NLWSKMQETNDWQKKYLSDGLHLTPEGNGVVFDEVSRVFREAWLSPEEMPFDFPHHSHID 229

Query: 135 FNNPLQAFEK 142
             NP +AFE+
Sbjct: 230 GKNPSKAFEE 239

BLAST of Cucsat.G2035 vs. ExPASy Swiss-Prot
Match: Q9SRM5 (GDSL esterase/lipase CPRD49 OS=Arabidopsis thaliana OX=3702 GN=CPRD49 PE=2 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 8.4e-14
Identity = 36/103 (34.95%), Postives = 62/103 (60.19%), Query Frame = 0

Query: 17  TRILLITPPPIDEEGRLQN--PYVSNPVNEPERTNDAAGAYAKACIAVAKECGVSVIDIW 76
           TRI+ ++ PP+DE    QN  PY+S  +    RTND    Y+ AC+ + +E G+ V+D++
Sbjct: 116 TRIIFLSSPPVDEAKVRQNQSPYLSEVI----RTNDLCKTYSDACVELCQELGLEVVDLF 175

Query: 77  TKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSP 118
           +  Q+   W+  C +DG+HL+  G+ IV  E+++ ++E    P
Sbjct: 176 STFQKADDWKTVCFTDGIHLSAQGSKIVAGEILRVVKEAEWHP 214

BLAST of Cucsat.G2035 vs. ExPASy Swiss-Prot
Match: Q711G3 (Isoamyl acetate-hydrolyzing esterase 1 homolog OS=Rattus norvegicus OX=10116 GN=Iah1 PE=2 SV=2)

HSP 1 Score: 74.7 bits (182), Expect = 9.3e-13
Identity = 45/121 (37.19%), Postives = 67/121 (55.37%), Query Frame = 0

Query: 15  PATRILLITPPPIDEEGRLQNPYVSNPVNEPERTNDAAGAYAKACIAVAKECGVSVIDIW 74
           P  R++LITPPP+ E    +   +     +  R N A G YAKAC+ VA++CG  V+D+W
Sbjct: 124 PKERVILITPPPLCEAAWEKECILKG--CKLNRLNVAVGEYAKACLQVARDCGTDVLDLW 183

Query: 75  TKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPVTLPADLPLISEIDFNN 134
           T MQ+      + LSDGLHL+  GN  +F  +   L+++  S   LP  LP   +++   
Sbjct: 184 TLMQKDNQDFSSYLSDGLHLSPLGNEFLFFHLWPLLDKKVSS---LPRLLPDWKDVEETK 239

Query: 135 P 136
           P
Sbjct: 244 P 239

BLAST of Cucsat.G2035 vs. ExPASy Swiss-Prot
Match: Q9DB29 (Isoamyl acetate-hydrolyzing esterase 1 homolog OS=Mus musculus OX=10090 GN=Iah1 PE=1 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 2.1e-12
Identity = 43/121 (35.54%), Postives = 65/121 (53.72%), Query Frame = 0

Query: 15  PATRILLITPPPIDEEGRLQNPYVSNPVNEPERTNDAAGAYAKACIAVAKECGVSVIDIW 74
           P  R++LITPPP+ E    +   +     +  R N   G YA AC+ VA++CG  V+D+W
Sbjct: 124 PRERVILITPPPLCEAAWEKECVLKG--CKLNRLNSVVGEYANACLQVARDCGTDVLDLW 183

Query: 75  TKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPVTLPADLPLISEIDFNN 134
           T MQ+      + LSDGLHL+  GN  +F  +   L+++  S   LP  LP   +++   
Sbjct: 184 TLMQKDSQDFSSYLSDGLHLSPMGNEFLFLNLCPLLDKKVSS---LPWLLPYWKDVEEAK 239

Query: 135 P 136
           P
Sbjct: 244 P 239

BLAST of Cucsat.G2035 vs. NCBI nr
Match: XP_004134670.1 (GDSL esterase/lipase At5g45920 [Cucumis sativus] >KGN49288.1 hypothetical protein Csa_003126 [Cucumis sativus])

HSP 1 Score: 274 bits (700), Expect = 4.65e-91
Identity = 132/133 (99.25%), Postives = 133/133 (100.00%), Query Frame = 0

Query: 10  FQKKWPATRILLITPPPIDEEGRLQNPYVSNPVNEPERTNDAAGAYAKACIAVAKECGVS 69
           F+KKWPATRILLITPPPIDEEGRLQNPYVSNPVNEPERTNDAAGAYAKACIAVAKECGVS
Sbjct: 108 FKKKWPATRILLITPPPIDEEGRLQNPYVSNPVNEPERTNDAAGAYAKACIAVAKECGVS 167

Query: 70  VIDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPVTLPADLPLISE 129
           VIDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPVTLPADLPLISE
Sbjct: 168 VIDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPVTLPADLPLISE 227

Query: 130 IDFNNPLQAFEKL 142
           IDFNNPLQAFEKL
Sbjct: 228 IDFNNPLQAFEKL 240

BLAST of Cucsat.G2035 vs. NCBI nr
Match: TYK13191.1 (GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 257 bits (656), Expect = 2.30e-84
Identity = 123/133 (92.48%), Postives = 127/133 (95.49%), Query Frame = 0

Query: 10  FQKKWPATRILLITPPPIDEEGRLQNPYVSNPVNEPERTNDAAGAYAKACIAVAKECGVS 69
           F+KKWPATRILLITPPPIDEEGRLQNPYV NP+NEPERTNDAAGAYA ACIAVAKECGVS
Sbjct: 108 FKKKWPATRILLITPPPIDEEGRLQNPYVPNPLNEPERTNDAAGAYANACIAVAKECGVS 167

Query: 70  VIDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPVTLPADLPLISE 129
           VIDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSP TLPADLPL SE
Sbjct: 168 VIDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPETLPADLPLFSE 227

Query: 130 IDFNNPLQAFEKL 142
           +D  NPL+AFEKL
Sbjct: 228 MDSTNPLKAFEKL 240

BLAST of Cucsat.G2035 vs. NCBI nr
Match: XP_008439764.1 (PREDICTED: GDSL esterase/lipase At5g45920 [Cucumis melo])

HSP 1 Score: 254 bits (650), Expect = 1.88e-83
Identity = 122/132 (92.42%), Postives = 126/132 (95.45%), Query Frame = 0

Query: 11  QKKWPATRILLITPPPIDEEGRLQNPYVSNPVNEPERTNDAAGAYAKACIAVAKECGVSV 70
           +KKWPATRILLITPPPIDEEGRLQNPYV NP+NEPERTNDAAGAYA ACIAVAKECGVSV
Sbjct: 109 KKKWPATRILLITPPPIDEEGRLQNPYVPNPLNEPERTNDAAGAYANACIAVAKECGVSV 168

Query: 71  IDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPVTLPADLPLISEI 130
           IDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSP TLPADLPL SE+
Sbjct: 169 IDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPETLPADLPLFSEM 228

Query: 131 DFNNPLQAFEKL 142
           D  NPL+AFEKL
Sbjct: 229 DSTNPLKAFEKL 240

BLAST of Cucsat.G2035 vs. NCBI nr
Match: XP_038882981.1 (GDSL esterase/lipase At5g45920 [Benincasa hispida] >XP_038882982.1 GDSL esterase/lipase At5g45920 [Benincasa hispida])

HSP 1 Score: 247 bits (631), Expect = 1.72e-80
Identity = 117/133 (87.97%), Postives = 126/133 (94.74%), Query Frame = 0

Query: 10  FQKKWPATRILLITPPPIDEEGRLQNPYVSNPVNEPERTNDAAGAYAKACIAVAKECGVS 69
           F+KKWPATRIL+ITPPPIDEEGRLQNPYVSNP+NEPERTN+AAG YAKACIAVA ECG+S
Sbjct: 113 FKKKWPATRILIITPPPIDEEGRLQNPYVSNPLNEPERTNNAAGDYAKACIAVAAECGIS 172

Query: 70  VIDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPVTLPADLPLISE 129
           VIDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVK+LEEEGLSP  LPA+LPL SE
Sbjct: 173 VIDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKKLEEEGLSPEKLPAELPLFSE 232

Query: 130 IDFNNPLQAFEKL 142
           ID NNPL+AF +L
Sbjct: 233 IDSNNPLKAFGEL 245

BLAST of Cucsat.G2035 vs. NCBI nr
Match: XP_022978753.1 (GDSL esterase/lipase At5g45920 [Cucurbita maxima])

HSP 1 Score: 241 bits (615), Expect = 4.22e-78
Identity = 112/132 (84.85%), Postives = 127/132 (96.21%), Query Frame = 0

Query: 11  QKKWPATRILLITPPPIDEEGRLQNPYVSNPVNEPERTNDAAGAYAKACIAVAKECGVSV 70
           +K+WPATRILLITPPPIDEEGRLQNPY+ NP+NEPERTN+AAG YAKACIAVA ECGVSV
Sbjct: 110 KKQWPATRILLITPPPIDEEGRLQNPYIVNPLNEPERTNNAAGDYAKACIAVAAECGVSV 169

Query: 71  IDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPVTLPADLPLISEI 130
           IDIWTKMQQ+PGWEKACLSDGLHL+RNGNTIVFEEVV++L+EEGLSP  LPADLPL+SEI
Sbjct: 170 IDIWTKMQQLPGWEKACLSDGLHLSRNGNTIVFEEVVQKLKEEGLSPEMLPADLPLLSEI 229

Query: 131 DFNNPLQAFEKL 142
           D+N+PL+AFE+L
Sbjct: 230 DYNDPLKAFEEL 241

BLAST of Cucsat.G2035 vs. ExPASy TrEMBL
Match: A0A0A0KI83 (SGNH_hydro domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G518920 PE=4 SV=1)

HSP 1 Score: 274 bits (700), Expect = 2.25e-91
Identity = 132/133 (99.25%), Postives = 133/133 (100.00%), Query Frame = 0

Query: 10  FQKKWPATRILLITPPPIDEEGRLQNPYVSNPVNEPERTNDAAGAYAKACIAVAKECGVS 69
           F+KKWPATRILLITPPPIDEEGRLQNPYVSNPVNEPERTNDAAGAYAKACIAVAKECGVS
Sbjct: 108 FKKKWPATRILLITPPPIDEEGRLQNPYVSNPVNEPERTNDAAGAYAKACIAVAKECGVS 167

Query: 70  VIDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPVTLPADLPLISE 129
           VIDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPVTLPADLPLISE
Sbjct: 168 VIDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPVTLPADLPLISE 227

Query: 130 IDFNNPLQAFEKL 142
           IDFNNPLQAFEKL
Sbjct: 228 IDFNNPLQAFEKL 240

BLAST of Cucsat.G2035 vs. ExPASy TrEMBL
Match: A0A5D3CMZ1 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G008030 PE=4 SV=1)

HSP 1 Score: 257 bits (656), Expect = 1.12e-84
Identity = 123/133 (92.48%), Postives = 127/133 (95.49%), Query Frame = 0

Query: 10  FQKKWPATRILLITPPPIDEEGRLQNPYVSNPVNEPERTNDAAGAYAKACIAVAKECGVS 69
           F+KKWPATRILLITPPPIDEEGRLQNPYV NP+NEPERTNDAAGAYA ACIAVAKECGVS
Sbjct: 108 FKKKWPATRILLITPPPIDEEGRLQNPYVPNPLNEPERTNDAAGAYANACIAVAKECGVS 167

Query: 70  VIDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPVTLPADLPLISE 129
           VIDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSP TLPADLPL SE
Sbjct: 168 VIDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPETLPADLPLFSE 227

Query: 130 IDFNNPLQAFEKL 142
           +D  NPL+AFEKL
Sbjct: 228 MDSTNPLKAFEKL 240

BLAST of Cucsat.G2035 vs. ExPASy TrEMBL
Match: A0A1S3B057 (GDSL esterase/lipase At5g45920 OS=Cucumis melo OX=3656 GN=LOC103484466 PE=4 SV=1)

HSP 1 Score: 254 bits (650), Expect = 9.12e-84
Identity = 122/132 (92.42%), Postives = 126/132 (95.45%), Query Frame = 0

Query: 11  QKKWPATRILLITPPPIDEEGRLQNPYVSNPVNEPERTNDAAGAYAKACIAVAKECGVSV 70
           +KKWPATRILLITPPPIDEEGRLQNPYV NP+NEPERTNDAAGAYA ACIAVAKECGVSV
Sbjct: 109 KKKWPATRILLITPPPIDEEGRLQNPYVPNPLNEPERTNDAAGAYANACIAVAKECGVSV 168

Query: 71  IDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPVTLPADLPLISEI 130
           IDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSP TLPADLPL SE+
Sbjct: 169 IDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPETLPADLPLFSEM 228

Query: 131 DFNNPLQAFEKL 142
           D  NPL+AFEKL
Sbjct: 229 DSTNPLKAFEKL 240

BLAST of Cucsat.G2035 vs. ExPASy TrEMBL
Match: A0A6J1IU69 (GDSL esterase/lipase At5g45920 OS=Cucurbita maxima OX=3661 GN=LOC111478618 PE=3 SV=1)

HSP 1 Score: 241 bits (615), Expect = 2.05e-78
Identity = 112/132 (84.85%), Postives = 127/132 (96.21%), Query Frame = 0

Query: 11  QKKWPATRILLITPPPIDEEGRLQNPYVSNPVNEPERTNDAAGAYAKACIAVAKECGVSV 70
           +K+WPATRILLITPPPIDEEGRLQNPY+ NP+NEPERTN+AAG YAKACIAVA ECGVSV
Sbjct: 110 KKQWPATRILLITPPPIDEEGRLQNPYIVNPLNEPERTNNAAGDYAKACIAVAAECGVSV 169

Query: 71  IDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPVTLPADLPLISEI 130
           IDIWTKMQQ+PGWEKACLSDGLHL+RNGNTIVFEEVV++L+EEGLSP  LPADLPL+SEI
Sbjct: 170 IDIWTKMQQLPGWEKACLSDGLHLSRNGNTIVFEEVVQKLKEEGLSPEMLPADLPLLSEI 229

Query: 131 DFNNPLQAFEKL 142
           D+N+PL+AFE+L
Sbjct: 230 DYNDPLKAFEEL 241

BLAST of Cucsat.G2035 vs. ExPASy TrEMBL
Match: A0A6J1ED78 (GDSL esterase/lipase At5g45920 OS=Cucurbita moschata OX=3662 GN=LOC111433110 PE=3 SV=1)

HSP 1 Score: 240 bits (612), Expect = 5.85e-78
Identity = 111/132 (84.09%), Postives = 127/132 (96.21%), Query Frame = 0

Query: 11  QKKWPATRILLITPPPIDEEGRLQNPYVSNPVNEPERTNDAAGAYAKACIAVAKECGVSV 70
           +K+WPATRILLITPPPIDEEGRLQNPY+ NP+NEPERTN+AAG YAKACIAVA ECGVSV
Sbjct: 110 KKQWPATRILLITPPPIDEEGRLQNPYLENPLNEPERTNNAAGDYAKACIAVAAECGVSV 169

Query: 71  IDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPVTLPADLPLISEI 130
           IDIWTKMQQ+PGWEKACLSDGLHL+RNGNTIVFEEVV++L+EEGLSP  LPADLPL+SE+
Sbjct: 170 IDIWTKMQQLPGWEKACLSDGLHLSRNGNTIVFEEVVQKLKEEGLSPEMLPADLPLLSEM 229

Query: 131 DFNNPLQAFEKL 142
           D+N+PL+AFE+L
Sbjct: 230 DYNDPLKAFEEL 241

BLAST of Cucsat.G2035 vs. TAIR 10
Match: AT5G45920.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 176.8 bits (447), Expect = 1.2e-44
Identity = 77/131 (58.78%), Postives = 101/131 (77.10%), Query Frame = 0

Query: 11  QKKWPATRILLITPPPIDEEGRLQNPYVSNPVNEPERTNDAAGAYAKACIAVAKECGVSV 70
           + +WP T I+LITPPPIDEE RL+ PY+ N    PERTN+ AG YAKACIAVA+EC +SV
Sbjct: 110 KNRWPQTAIILITPPPIDEEARLRYPYIENTTGLPERTNEVAGLYAKACIAVAEECQISV 169

Query: 71  IDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPVTLPADLPLISEI 130
            D+W+KMQQ+P W+  CL DGLHL+R GN +VFEEV K+L+EEG+    L  DLPLI ++
Sbjct: 170 TDLWSKMQQIPNWQTECLWDGLHLSRVGNKVVFEEVAKKLKEEGIGAEDLAVDLPLIEDV 229

Query: 131 DFNNPLQAFEK 142
           D  +PL++F++
Sbjct: 230 DPKDPLKSFDE 240

BLAST of Cucsat.G2035 vs. TAIR 10
Match: AT5G62930.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 130.6 bits (327), Expect = 1.0e-30
Identity = 63/130 (48.46%), Postives = 85/130 (65.38%), Query Frame = 0

Query: 15  PATRILLITPPPIDEEGR---LQNPYVSNPVNEPERTNDAAGAYAKACIAVAKECGVSVI 74
           P   I+LITPPPIDE GR    ++ Y    + EPERTN+  G YA+ C+A+A+E G+  +
Sbjct: 110 PTMLIVLITPPPIDEAGRQSYAESIYGEKAMKEPERTNETTGVYAQHCVALAEELGLRCV 169

Query: 75  DIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPVTLPADLPLISEID 134
           ++W+KMQ+   W+K  LSDGLHLT  GN +VF+EV +   E  LSP  +P D P  S ID
Sbjct: 170 NLWSKMQETNDWQKKYLSDGLHLTPEGNGVVFDEVSRVFREAWLSPEEMPFDFPHHSHID 229

Query: 135 FNNPLQAFEK 142
             NP +AFE+
Sbjct: 230 GKNPSKAFEE 239

BLAST of Cucsat.G2035 vs. TAIR 10
Match: AT3G11210.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 78.2 bits (191), Expect = 6.0e-15
Identity = 36/103 (34.95%), Postives = 62/103 (60.19%), Query Frame = 0

Query: 17  TRILLITPPPIDEEGRLQN--PYVSNPVNEPERTNDAAGAYAKACIAVAKECGVSVIDIW 76
           TRI+ ++ PP+DE    QN  PY+S  +    RTND    Y+ AC+ + +E G+ V+D++
Sbjct: 116 TRIIFLSSPPVDEAKVRQNQSPYLSEVI----RTNDLCKTYSDACVELCQELGLEVVDLF 175

Query: 77  TKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSP 118
           +  Q+   W+  C +DG+HL+  G+ IV  E+++ ++E    P
Sbjct: 176 STFQKADDWKTVCFTDGIHLSAQGSKIVAGEILRVVKEAEWHP 214