Cucsat.G2035 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G2035
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	GDSL esterase/lipase
Location	ctg1002: 5788573 .. 5790595 (-)
RNA-Seq Expression	Cucsat.G2035
Synteny	Cucsat.G2035

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AAATAAAAAATTCTTGGTTGATTAGACCTTTCATTTTCTAGAAAAACAAAAATTCAAGCAAAATCTACATATGTTAGTGATGGACTCAATTATTGATGAGATGAATCATTTCTAATGATCTATTGGTTAGTATTTTGGACGTTCGACATTTGTCTAAACGTTGCTATATTCTTCATAAAAACTACCATACTTGGAGAAAAAGAAGGTCGAGAAGTATATAGTTTTATATAAACTTCTAAAATTTACGATATTAAAAAAATGTCGAAAAACATTTGGAGCTGGAATTTCCTGATTATAGTAATGTGGGAGATTCAATATTTGGAAGTGATAATCTTCGGGATGCTTCTTTGCAAATAACCCATTTGAAAGATGAGACCAAAGATCTATCTTTTTGGCGATTCCATTACAGAATTTTCTTTCGCTGATGGCGGCTGGGGATCCGCTCTAACCAATCATTTCACTCGCAAGGTTCGGCTGCTTTTCTTTTCTTCTCTTTGTTTTTTTCTTCCACTGTTTGCGTTAACCCGCTATAAAATTTGATTCCCAACTTCCCTTTCATCATCTGTTTCGAGTCTGAAAATTTTTGGGTGTTTTTCTTTTGTTTGAATGTGATTAGGCCGATGTGGTGGTTAGAGGATACAGTGGCTACAACACGAGATGGGCGCTTAAGGTCATTGAACGAGTCTTCCCGCCATCAGAAGAACGTGAAGCTGCCTCGCCGCTTGCGGTGACGGTGTTCTTTGGAGCTAATGACGCTTGTCTTCCAGATAGATATGGCGCCTTCCAACATGTGCCCCTTCATGAATACAAGCAAAACCTCATTTCAATCGTCTCCTTTTTCAAGGTATATTCTTGCTTCACGGCTGCAATGTTGTTTCCCCACATATGTAGAATCTGTCGTTGCCGAAGTGTAAGCTATTTTTCGTTGCCTGGTTATTCTTTATTGATTTAGCCAAGGATTTGTTGAAAGGGCCCTCCGGTTCATCCCAAAATTTTGGATTTATTGTGTTGGAATTTGAACATTGGCTAGAAGTGGAGAAGATGTTAGATTGGATATCGAATGAAGGGCGATGAATTTTAAAGTAAAACAATGTGAGATTTGGGCCCAAAGGGATCGGGGGATGGTGTTATACTATTTAAAGATGTGATTTATATGTGTTAGCCTAATATTTTTTCCAAGGAAAATTAGCAGCTTTTTTGGGAAAGACACCCTTTTGGTTGGCTTGAATAAGGAGGATCACTTTTAATTGGCTTTTCTTTTGATGATTAGAATTTGTTTTTTGTTGGATTCTCTTACTGTAATGGTAAATGCTTTGTAGGTGAAGTTTTACTTTATAATGTGACTATACCTTTATTCTAAATCTTCTTGTGAAAACTTTTTGTTATTGAAATTAAGTATAGTGTTTCGGTTGTGGTGCCTACATGTTCTTCTATTCACAAACAAAACAACAAACACAATAATTTTTATCCATTGAACTTATCTTTTCTAGCAATTCAAGTCGAGTCTAAGTTTGTTTCTATTGGATTTTCAGAAGAAATGGCCTGCAACACGTATTTTGCTTATCACTCCTCCTCCTATTGATGAAGAAGGGCGTTTACAGTATGGTTATCTATCTCTCCCACTTTCTTTGTTTGGCACAAATTATTTTGAGAGATCTCGATTCTCTCAGTTTTTTAATTGTAGTTCTGATAATGGGAAATGATACAATCTCTGCAGGAACCCTTATGTTTCAAATCCAGTAAATGAGCCTGAGAGGACTAACGATGCTGCTGGTGCTTATGCCAAGGCTTGCATTGCTGTTGCTAAAGAATGTGGGGTTTCTGTTATTGATATTTGGACTAAGATGCAGCAAGTTCCTGGCTGGGAAAAAGCTTGTTTAAGGTATTCATAGATTCACTCTTATGTTTCTGATATCTTTTACATAAGCAATTGATTCGTATATTCTACTTGCATCATGACCATCTATTAGTATGTTTTGTTGGACTCTGATTATGTATAGATTTCGGAAATAACAAGATGTGGAATGATTTCAGTATATTAGAATTTGTAAACTTGGACAAGAGTACATTTTTGCCACATCACGTGGCCATAAAATTAGATGTAATAGAGTGCTTAAGCTTAAGTTGCCCTCTTCCCCTCGTGTTGGTGGACAGTTAGGAACTGTCAAAACTCTTATCTTTCCTAACAATTTCGTTCTGTATTCTCTTAAATATCTGGCCATTGAAAAGCTCTGGAGCTATTGTTCAGATTGCTAAAACAAAACAAAAATGAGGCTATTCAGTATGAAACGCTTAGTTAGAGAGGACTAATACTTCACTCGTAAGTCACATTTTACCCTTGGCTAATATGATACTATATCTCTACTTTGCCTTTCAACAAGAACTATTTGTTTCTGTTTCAGTTTTTTACAATGGAATTTGTTTTATCATTATTTCTTTATGATGATTTTATTCTTAGGGAATCATTTGAACATTTTGCCAAATTCCAAAAACCAGAACAAGTTTTTAAAGACTAATTTTTTTAGTTTTCAAAATTTTAAAAGCATTTGTAAAACATAGATAACAAAGAAAAAAAATCATATGCAGAAGTAATTTTCATAAGCCATGAACCACAAGCTAAATGGTTATCAAAAGGGGGTGTTAGTTAATCTTTGCATGTGCTGGTGATGGCTTCTACTCCCCGGTTTTCCCTTTCACTCTTCTCGAAAAGGTTTACCAAACAGGCGAGATTGGGACATAGCAAAACTAGCTAGCTCAGACATTTGGTGCTGGCATGCTGCTCTGAGCTTATATTAAGAGCCTATAGCATGCATATCTGTTGCTCGTGGTTCTGCTTAAGATGCTGTCTGAGTGTCTTTTCTTCCGATATTTAGAAACTTCTTTATGATCATGTTTATCATTAATGTGCATCAGCATTCCTGGTTTGTGTTCTCTCTTATCCTTACTCCTAAGTTAAAAATACCTTTGAAAACAAACTTGAATGAAAAGTAACTTGGAAAGATTATCTATCAAGTCAGATTTTTGTATTAGAAGCATCTAGATTTCATAGAAATTAGAACAACTTATTATATGAAACCTTCACTCTTTGCTCTACAAGATCTGAACCTAATCTATCCATTTGTTTGCAGTGATGGACTGCACCTGACTCGAAATGGAAACACGATTGTGTTCGAGGAAGTTGTCAAGAGGCTGGAAGAAGAAGGATTGAGTCCAGTAACGTTGCCTGCTGATCTTCCACTTATTTCAGAAATAGACTTTAATAATCCACTGCAAGCTTTCGAAAAATTATAATAATCAGTTAACTGAAACCGAGGATGCCATCGAGGACGAGGAGCACTGCAACTTATTCACAACCATTGAGATGTGAGAATGAAATTGAAACTCCTCTACAGATGATAATAATGCTTAATTACCATTAAGAGTTGGATTTCATTCGTTCAAAACTTCTTTATTAGACATCTATGGGATTGTGAGCATGCAGATCTGTCAATAAATAAATGGTCTTTCTCCAATTATACAACATACAATTCGATGGATAATTAGTAATGATATTAACCAAACAGGGATAAAAC

Coding sequence (CDS)

ATGAGACCAAAGATCTATCTTTTTGGCGATTCCATTACAGAATTTTCTTTCGCTGATGGCGGCTGGGGATCCGCTCTAACCAATCATTTCACTCGCAAGGCCGATGTGGTGGTTAGAGGATACAGTGGCTACAACACGAGATGGGCGCTTAAGGTCATTGAACGAGTCTTCCCGCCATCAGAAGAACGTGAAGCTGCCTCGCCGCTTGCGGTGACGGTGTTCTTTGGAGCTAATGACGCTTGTCTTCCAGATAGATATGGCGCCTTCCAACATGTGCCCCTTCATGAATACAAGCAAAACCTCATTTCAATCGTCTCCTTTTTCAAGAAGAAATGGCCTGCAACACGTATTTTGCTTATCACTCCTCCTCCTATTGATGAAGAAGGGCGTTTACAGAACCCTTATGTTTCAAATCCAGTAAATGAGCCTGAGAGGACTAACGATGCTGCTGGTGCTTATGCCAAGGCTTGCATTGCTGTTGCTAAAGAATGTGGGGTTTCTGTTATTGATATTTGGACTAAGATGCAGCAAGTTCCTGGCTGGGAAAAAGCTTGTTTAAGTGATGGACTGCACCTGACTCGAAATGGAAACACGATTGTGTTCGAGGAAGTTGTCAAGAGGCTGGAAGAAGAAGGATTGAGTCCAGTAACGTTGCCTGCTGATCTTCCACTTATTTCAGAAATAGACTTTAATAATCCACTGCAAGCTTTCGAAAAATTATAA

Protein sequence

MRPKIYLFGDSITEFSFADGGWGSALTNHFTRKADVVVRGYSGYNTRWALKVIERVFPPSEEREAASPLAVTVFFGANDACLPDRYGAFQHVPLHEYKQNLISIVSFFKKKWPATRILLITPPPIDEEGRLQNPYVSNPVNEPERTNDAAGAYAKACIAVAKECGVSVIDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPVTLPADLPLISEIDFNNPLQAFEKL

Homology

[Close]

BLAST of Cucsat.G2035 vs. ExPASy Swiss-Prot
Match: Q6NMR9 (GDSL esterase/lipase At5g45920 OS=Arabidopsis thaliana OX=3702 GN=At5g45920 PE=2 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 1.7e-43
Identity = 77/131 (58.78%), Postives = 101/131 (77.10%), Query Frame = 0


Query: 11  QKKWPATRILLITPPPIDEEGRLQNPYVSNPVNEPERTNDAAGAYAKACIAVAKECGVSV 70
           + +WP T I+LITPPPIDEE RL+ PY+ N    PERTN+ AG YAKACIAVA+EC +SV
Sbjct: 110 KNRWPQTAIILITPPPIDEEARLRYPYIENTTGLPERTNEVAGLYAKACIAVAEECQISV 169

Query: 71  IDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPVTLPADLPLISEI 130
            D+W+KMQQ+P W+  CL DGLHL+R GN +VFEEV K+L+EEG+    L  DLPLI ++
Sbjct: 170 TDLWSKMQQIPNWQTECLWDGLHLSRVGNKVVFEEVAKKLKEEGIGAEDLAVDLPLIEDV 229

Query: 131 DFNNPLQAFEK 142
           D  +PL++F++
Sbjct: 230 DPKDPLKSFDE 240

[Close]

BLAST of Cucsat.G2035 vs. ExPASy Swiss-Prot
Match: Q9FM04 (GDSL esterase/lipase At5g62930 OS=Arabidopsis thaliana OX=3702 GN=At5g62930 PE=2 SV=3)

HSP 1 Score: 130.6 bits (327), Expect = 1.4e-29
Identity = 63/130 (48.46%), Postives = 85/130 (65.38%), Query Frame = 0


Query: 15  PATRILLITPPPIDEEGR---LQNPYVSNPVNEPERTNDAAGAYAKACIAVAKECGVSVI 74
           P   I+LITPPPIDE GR    ++ Y    + EPERTN+  G YA+ C+A+A+E G+  +
Sbjct: 110 PTMLIVLITPPPIDEAGRQSYAESIYGEKAMKEPERTNETTGVYAQHCVALAEELGLRCV 169

Query: 75  DIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPVTLPADLPLISEID 134
           ++W+KMQ+   W+K  LSDGLHLT  GN +VF+EV +   E  LSP  +P D P  S ID
Sbjct: 170 NLWSKMQETNDWQKKYLSDGLHLTPEGNGVVFDEVSRVFREAWLSPEEMPFDFPHHSHID 229

Query: 135 FNNPLQAFEK 142
             NP +AFE+
Sbjct: 230 GKNPSKAFEE 239

[Close]

BLAST of Cucsat.G2035 vs. ExPASy Swiss-Prot
Match: Q9SRM5 (GDSL esterase/lipase CPRD49 OS=Arabidopsis thaliana OX=3702 GN=CPRD49 PE=2 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 8.4e-14
Identity = 36/103 (34.95%), Postives = 62/103 (60.19%), Query Frame = 0


Query: 17  TRILLITPPPIDEEGRLQN--PYVSNPVNEPERTNDAAGAYAKACIAVAKECGVSVIDIW 76
           TRI+ ++ PP+DE    QN  PY+S  +    RTND    Y+ AC+ + +E G+ V+D++
Sbjct: 116 TRIIFLSSPPVDEAKVRQNQSPYLSEVI----RTNDLCKTYSDACVELCQELGLEVVDLF 175

Query: 77  TKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSP 118
           +  Q+   W+  C +DG+HL+  G+ IV  E+++ ++E    P
Sbjct: 176 STFQKADDWKTVCFTDGIHLSAQGSKIVAGEILRVVKEAEWHP 214

[Close]

BLAST of Cucsat.G2035 vs. ExPASy Swiss-Prot
Match: Q711G3 (Isoamyl acetate-hydrolyzing esterase 1 homolog OS=Rattus norvegicus OX=10116 GN=Iah1 PE=2 SV=2)

HSP 1 Score: 74.7 bits (182), Expect = 9.3e-13
Identity = 45/121 (37.19%), Postives = 67/121 (55.37%), Query Frame = 0


Query: 15  PATRILLITPPPIDEEGRLQNPYVSNPVNEPERTNDAAGAYAKACIAVAKECGVSVIDIW 74
           P  R++LITPPP+ E    +   +     +  R N A G YAKAC+ VA++CG  V+D+W
Sbjct: 124 PKERVILITPPPLCEAAWEKECILKG--CKLNRLNVAVGEYAKACLQVARDCGTDVLDLW 183

Query: 75  TKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPVTLPADLPLISEIDFNN 134
           T MQ+      + LSDGLHL+  GN  +F  +   L+++  S   LP  LP   +++   
Sbjct: 184 TLMQKDNQDFSSYLSDGLHLSPLGNEFLFFHLWPLLDKKVSS---LPRLLPDWKDVEETK 239

Query: 135 P 136
           P
Sbjct: 244 P 239

[Close]

BLAST of Cucsat.G2035 vs. ExPASy Swiss-Prot
Match: Q9DB29 (Isoamyl acetate-hydrolyzing esterase 1 homolog OS=Mus musculus OX=10090 GN=Iah1 PE=1 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 2.1e-12
Identity = 43/121 (35.54%), Postives = 65/121 (53.72%), Query Frame = 0


Query: 15  PATRILLITPPPIDEEGRLQNPYVSNPVNEPERTNDAAGAYAKACIAVAKECGVSVIDIW 74
           P  R++LITPPP+ E    +   +     +  R N   G YA AC+ VA++CG  V+D+W
Sbjct: 124 PRERVILITPPPLCEAAWEKECVLKG--CKLNRLNSVVGEYANACLQVARDCGTDVLDLW 183

Query: 75  TKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPVTLPADLPLISEIDFNN 134
           T MQ+      + LSDGLHL+  GN  +F  +   L+++  S   LP  LP   +++   
Sbjct: 184 TLMQKDSQDFSSYLSDGLHLSPMGNEFLFLNLCPLLDKKVSS---LPWLLPYWKDVEEAK 239

Query: 135 P 136
           P
Sbjct: 244 P 239

[Close]

BLAST of Cucsat.G2035 vs. NCBI nr
Match: XP_004134670.1 (GDSL esterase/lipase At5g45920 [Cucumis sativus] >KGN49288.1 hypothetical protein Csa_003126 [Cucumis sativus])

HSP 1 Score: 274 bits (700), Expect = 4.65e-91
Identity = 132/133 (99.25%), Postives = 133/133 (100.00%), Query Frame = 0


Query: 10  FQKKWPATRILLITPPPIDEEGRLQNPYVSNPVNEPERTNDAAGAYAKACIAVAKECGVS 69
           F+KKWPATRILLITPPPIDEEGRLQNPYVSNPVNEPERTNDAAGAYAKACIAVAKECGVS
Sbjct: 108 FKKKWPATRILLITPPPIDEEGRLQNPYVSNPVNEPERTNDAAGAYAKACIAVAKECGVS 167

Query: 70  VIDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPVTLPADLPLISE 129
           VIDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPVTLPADLPLISE
Sbjct: 168 VIDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPVTLPADLPLISE 227

Query: 130 IDFNNPLQAFEKL 142
           IDFNNPLQAFEKL
Sbjct: 228 IDFNNPLQAFEKL 240

[Close]

BLAST of Cucsat.G2035 vs. NCBI nr
Match: TYK13191.1 (GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 257 bits (656), Expect = 2.30e-84
Identity = 123/133 (92.48%), Postives = 127/133 (95.49%), Query Frame = 0


Query: 10  FQKKWPATRILLITPPPIDEEGRLQNPYVSNPVNEPERTNDAAGAYAKACIAVAKECGVS 69
           F+KKWPATRILLITPPPIDEEGRLQNPYV NP+NEPERTNDAAGAYA ACIAVAKECGVS
Sbjct: 108 FKKKWPATRILLITPPPIDEEGRLQNPYVPNPLNEPERTNDAAGAYANACIAVAKECGVS 167

Query: 70  VIDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPVTLPADLPLISE 129
           VIDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSP TLPADLPL SE
Sbjct: 168 VIDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPETLPADLPLFSE 227

Query: 130 IDFNNPLQAFEKL 142
           +D  NPL+AFEKL
Sbjct: 228 MDSTNPLKAFEKL 240

[Close]

BLAST of Cucsat.G2035 vs. NCBI nr
Match: XP_008439764.1 (PREDICTED: GDSL esterase/lipase At5g45920 [Cucumis melo])

HSP 1 Score: 254 bits (650), Expect = 1.88e-83
Identity = 122/132 (92.42%), Postives = 126/132 (95.45%), Query Frame = 0


Query: 11  QKKWPATRILLITPPPIDEEGRLQNPYVSNPVNEPERTNDAAGAYAKACIAVAKECGVSV 70
           +KKWPATRILLITPPPIDEEGRLQNPYV NP+NEPERTNDAAGAYA ACIAVAKECGVSV
Sbjct: 109 KKKWPATRILLITPPPIDEEGRLQNPYVPNPLNEPERTNDAAGAYANACIAVAKECGVSV 168

Query: 71  IDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPVTLPADLPLISEI 130
           IDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSP TLPADLPL SE+
Sbjct: 169 IDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPETLPADLPLFSEM 228

Query: 131 DFNNPLQAFEKL 142
           D  NPL+AFEKL
Sbjct: 229 DSTNPLKAFEKL 240

[Close]

BLAST of Cucsat.G2035 vs. NCBI nr
Match: XP_038882981.1 (GDSL esterase/lipase At5g45920 [Benincasa hispida] >XP_038882982.1 GDSL esterase/lipase At5g45920 [Benincasa hispida])

HSP 1 Score: 247 bits (631), Expect = 1.72e-80
Identity = 117/133 (87.97%), Postives = 126/133 (94.74%), Query Frame = 0


Query: 10  FQKKWPATRILLITPPPIDEEGRLQNPYVSNPVNEPERTNDAAGAYAKACIAVAKECGVS 69
           F+KKWPATRIL+ITPPPIDEEGRLQNPYVSNP+NEPERTN+AAG YAKACIAVA ECG+S
Sbjct: 113 FKKKWPATRILIITPPPIDEEGRLQNPYVSNPLNEPERTNNAAGDYAKACIAVAAECGIS 172

Query: 70  VIDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPVTLPADLPLISE 129
           VIDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVK+LEEEGLSP  LPA+LPL SE
Sbjct: 173 VIDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKKLEEEGLSPEKLPAELPLFSE 232

Query: 130 IDFNNPLQAFEKL 142
           ID NNPL+AF +L
Sbjct: 233 IDSNNPLKAFGEL 245

[Close]

BLAST of Cucsat.G2035 vs. NCBI nr
Match: XP_022978753.1 (GDSL esterase/lipase At5g45920 [Cucurbita maxima])

HSP 1 Score: 241 bits (615), Expect = 4.22e-78
Identity = 112/132 (84.85%), Postives = 127/132 (96.21%), Query Frame = 0


Query: 11  QKKWPATRILLITPPPIDEEGRLQNPYVSNPVNEPERTNDAAGAYAKACIAVAKECGVSV 70
           +K+WPATRILLITPPPIDEEGRLQNPY+ NP+NEPERTN+AAG YAKACIAVA ECGVSV
Sbjct: 110 KKQWPATRILLITPPPIDEEGRLQNPYIVNPLNEPERTNNAAGDYAKACIAVAAECGVSV 169

Query: 71  IDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPVTLPADLPLISEI 130
           IDIWTKMQQ+PGWEKACLSDGLHL+RNGNTIVFEEVV++L+EEGLSP  LPADLPL+SEI
Sbjct: 170 IDIWTKMQQLPGWEKACLSDGLHLSRNGNTIVFEEVVQKLKEEGLSPEMLPADLPLLSEI 229

Query: 131 DFNNPLQAFEKL 142
           D+N+PL+AFE+L
Sbjct: 230 DYNDPLKAFEEL 241

[Close]

BLAST of Cucsat.G2035 vs. ExPASy TrEMBL
Match: A0A0A0KI83 (SGNH_hydro domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G518920 PE=4 SV=1)

HSP 1 Score: 274 bits (700), Expect = 2.25e-91
Identity = 132/133 (99.25%), Postives = 133/133 (100.00%), Query Frame = 0


Query: 10  FQKKWPATRILLITPPPIDEEGRLQNPYVSNPVNEPERTNDAAGAYAKACIAVAKECGVS 69
           F+KKWPATRILLITPPPIDEEGRLQNPYVSNPVNEPERTNDAAGAYAKACIAVAKECGVS
Sbjct: 108 FKKKWPATRILLITPPPIDEEGRLQNPYVSNPVNEPERTNDAAGAYAKACIAVAKECGVS 167

Query: 70  VIDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPVTLPADLPLISE 129
           VIDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPVTLPADLPLISE
Sbjct: 168 VIDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPVTLPADLPLISE 227

Query: 130 IDFNNPLQAFEKL 142
           IDFNNPLQAFEKL
Sbjct: 228 IDFNNPLQAFEKL 240

[Close]

BLAST of Cucsat.G2035 vs. ExPASy TrEMBL
Match: A0A5D3CMZ1 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G008030 PE=4 SV=1)

HSP 1 Score: 257 bits (656), Expect = 1.12e-84
Identity = 123/133 (92.48%), Postives = 127/133 (95.49%), Query Frame = 0


Query: 10  FQKKWPATRILLITPPPIDEEGRLQNPYVSNPVNEPERTNDAAGAYAKACIAVAKECGVS 69
           F+KKWPATRILLITPPPIDEEGRLQNPYV NP+NEPERTNDAAGAYA ACIAVAKECGVS
Sbjct: 108 FKKKWPATRILLITPPPIDEEGRLQNPYVPNPLNEPERTNDAAGAYANACIAVAKECGVS 167

Query: 70  VIDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPVTLPADLPLISE 129
           VIDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSP TLPADLPL SE
Sbjct: 168 VIDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPETLPADLPLFSE 227

Query: 130 IDFNNPLQAFEKL 142
           +D  NPL+AFEKL
Sbjct: 228 MDSTNPLKAFEKL 240

[Close]

BLAST of Cucsat.G2035 vs. ExPASy TrEMBL
Match: A0A1S3B057 (GDSL esterase/lipase At5g45920 OS=Cucumis melo OX=3656 GN=LOC103484466 PE=4 SV=1)

HSP 1 Score: 254 bits (650), Expect = 9.12e-84
Identity = 122/132 (92.42%), Postives = 126/132 (95.45%), Query Frame = 0


Query: 11  QKKWPATRILLITPPPIDEEGRLQNPYVSNPVNEPERTNDAAGAYAKACIAVAKECGVSV 70
           +KKWPATRILLITPPPIDEEGRLQNPYV NP+NEPERTNDAAGAYA ACIAVAKECGVSV
Sbjct: 109 KKKWPATRILLITPPPIDEEGRLQNPYVPNPLNEPERTNDAAGAYANACIAVAKECGVSV 168

Query: 71  IDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPVTLPADLPLISEI 130
           IDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSP TLPADLPL SE+
Sbjct: 169 IDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPETLPADLPLFSEM 228

Query: 131 DFNNPLQAFEKL 142
           D  NPL+AFEKL
Sbjct: 229 DSTNPLKAFEKL 240

[Close]

BLAST of Cucsat.G2035 vs. ExPASy TrEMBL
Match: A0A6J1IU69 (GDSL esterase/lipase At5g45920 OS=Cucurbita maxima OX=3661 GN=LOC111478618 PE=3 SV=1)

HSP 1 Score: 241 bits (615), Expect = 2.05e-78
Identity = 112/132 (84.85%), Postives = 127/132 (96.21%), Query Frame = 0


Query: 11  QKKWPATRILLITPPPIDEEGRLQNPYVSNPVNEPERTNDAAGAYAKACIAVAKECGVSV 70
           +K+WPATRILLITPPPIDEEGRLQNPY+ NP+NEPERTN+AAG YAKACIAVA ECGVSV
Sbjct: 110 KKQWPATRILLITPPPIDEEGRLQNPYIVNPLNEPERTNNAAGDYAKACIAVAAECGVSV 169

Query: 71  IDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPVTLPADLPLISEI 130
           IDIWTKMQQ+PGWEKACLSDGLHL+RNGNTIVFEEVV++L+EEGLSP  LPADLPL+SEI
Sbjct: 170 IDIWTKMQQLPGWEKACLSDGLHLSRNGNTIVFEEVVQKLKEEGLSPEMLPADLPLLSEI 229

Query: 131 DFNNPLQAFEKL 142
           D+N+PL+AFE+L
Sbjct: 230 DYNDPLKAFEEL 241

[Close]

BLAST of Cucsat.G2035 vs. ExPASy TrEMBL
Match: A0A6J1ED78 (GDSL esterase/lipase At5g45920 OS=Cucurbita moschata OX=3662 GN=LOC111433110 PE=3 SV=1)

HSP 1 Score: 240 bits (612), Expect = 5.85e-78
Identity = 111/132 (84.09%), Postives = 127/132 (96.21%), Query Frame = 0


Query: 11  QKKWPATRILLITPPPIDEEGRLQNPYVSNPVNEPERTNDAAGAYAKACIAVAKECGVSV 70
           +K+WPATRILLITPPPIDEEGRLQNPY+ NP+NEPERTN+AAG YAKACIAVA ECGVSV
Sbjct: 110 KKQWPATRILLITPPPIDEEGRLQNPYLENPLNEPERTNNAAGDYAKACIAVAAECGVSV 169

Query: 71  IDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPVTLPADLPLISEI 130
           IDIWTKMQQ+PGWEKACLSDGLHL+RNGNTIVFEEVV++L+EEGLSP  LPADLPL+SE+
Sbjct: 170 IDIWTKMQQLPGWEKACLSDGLHLSRNGNTIVFEEVVQKLKEEGLSPEMLPADLPLLSEM 229

Query: 131 DFNNPLQAFEKL 142
           D+N+PL+AFE+L
Sbjct: 230 DYNDPLKAFEEL 241

[Close]

BLAST of Cucsat.G2035 vs. TAIR 10
Match: AT5G45920.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 176.8 bits (447), Expect = 1.2e-44
Identity = 77/131 (58.78%), Postives = 101/131 (77.10%), Query Frame = 0


Query: 11  QKKWPATRILLITPPPIDEEGRLQNPYVSNPVNEPERTNDAAGAYAKACIAVAKECGVSV 70
           + +WP T I+LITPPPIDEE RL+ PY+ N    PERTN+ AG YAKACIAVA+EC +SV
Sbjct: 110 KNRWPQTAIILITPPPIDEEARLRYPYIENTTGLPERTNEVAGLYAKACIAVAEECQISV 169

Query: 71  IDIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPVTLPADLPLISEI 130
            D+W+KMQQ+P W+  CL DGLHL+R GN +VFEEV K+L+EEG+    L  DLPLI ++
Sbjct: 170 TDLWSKMQQIPNWQTECLWDGLHLSRVGNKVVFEEVAKKLKEEGIGAEDLAVDLPLIEDV 229

Query: 131 DFNNPLQAFEK 142
           D  +PL++F++
Sbjct: 230 DPKDPLKSFDE 240

[Close]

BLAST of Cucsat.G2035 vs. TAIR 10
Match: AT5G62930.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 130.6 bits (327), Expect = 1.0e-30
Identity = 63/130 (48.46%), Postives = 85/130 (65.38%), Query Frame = 0


Query: 15  PATRILLITPPPIDEEGR---LQNPYVSNPVNEPERTNDAAGAYAKACIAVAKECGVSVI 74
           P   I+LITPPPIDE GR    ++ Y    + EPERTN+  G YA+ C+A+A+E G+  +
Sbjct: 110 PTMLIVLITPPPIDEAGRQSYAESIYGEKAMKEPERTNETTGVYAQHCVALAEELGLRCV 169

Query: 75  DIWTKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSPVTLPADLPLISEID 134
           ++W+KMQ+   W+K  LSDGLHLT  GN +VF+EV +   E  LSP  +P D P  S ID
Sbjct: 170 NLWSKMQETNDWQKKYLSDGLHLTPEGNGVVFDEVSRVFREAWLSPEEMPFDFPHHSHID 229

Query: 135 FNNPLQAFEK 142
             NP +AFE+
Sbjct: 230 GKNPSKAFEE 239

[Close]

BLAST of Cucsat.G2035 vs. TAIR 10
Match: AT3G11210.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 78.2 bits (191), Expect = 6.0e-15
Identity = 36/103 (34.95%), Postives = 62/103 (60.19%), Query Frame = 0


Query: 17  TRILLITPPPIDEEGRLQN--PYVSNPVNEPERTNDAAGAYAKACIAVAKECGVSVIDIW 76
           TRI+ ++ PP+DE    QN  PY+S  +    RTND    Y+ AC+ + +E G+ V+D++
Sbjct: 116 TRIIFLSSPPVDEAKVRQNQSPYLSEVI----RTNDLCKTYSDACVELCQELGLEVVDLF 175

Query: 77  TKMQQVPGWEKACLSDGLHLTRNGNTIVFEEVVKRLEEEGLSP 118
           +  Q+   W+  C +DG+HL+  G+ IV  E+++ ++E    P
Sbjct: 176 STFQKADDWKTVCFTDGIHLSAQGSKIVAGEILRVVKEAEWHP 214

The following BLAST results are available for this feature:

BLAST of Cucsat.G2035 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6NMR9	1.7e-43	58.78	GDSL esterase/lipase At5g45920 OS=Arabidopsis thaliana OX=3702 GN=At5g45920 PE=2...	[more]
Q9FM04	1.4e-29	48.46	GDSL esterase/lipase At5g62930 OS=Arabidopsis thaliana OX=3702 GN=At5g62930 PE=2...	[more]
Q9SRM5	8.4e-14	34.95	GDSL esterase/lipase CPRD49 OS=Arabidopsis thaliana OX=3702 GN=CPRD49 PE=2 SV=1	[more]
Q711G3	9.3e-13	37.19	Isoamyl acetate-hydrolyzing esterase 1 homolog OS=Rattus norvegicus OX=10116 GN=...	[more]
Q9DB29	2.1e-12	35.54	Isoamyl acetate-hydrolyzing esterase 1 homolog OS=Mus musculus OX=10090 GN=Iah1 ...	[more]

BLAST of Cucsat.G2035 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004134670.1	4.65e-91	99.25	GDSL esterase/lipase At5g45920 [Cucumis sativus] >KGN49288.1 hypothetical protei...	[more]
TYK13191.1	2.30e-84	92.48	GDSL esterase/lipase [Cucumis melo var. makuwa]	[more]
XP_008439764.1	1.88e-83	92.42	PREDICTED: GDSL esterase/lipase At5g45920 [Cucumis melo]	[more]
XP_038882981.1	1.72e-80	87.97	GDSL esterase/lipase At5g45920 [Benincasa hispida] >XP_038882982.1 GDSL esterase...	[more]
XP_022978753.1	4.22e-78	84.85	GDSL esterase/lipase At5g45920 [Cucurbita maxima]	[more]

BLAST of Cucsat.G2035 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KI83	2.25e-91	99.25	SGNH_hydro domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G518920 ...	[more]
A0A5D3CMZ1	1.12e-84	92.48	GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255...	[more]
A0A1S3B057	9.12e-84	92.42	GDSL esterase/lipase At5g45920 OS=Cucumis melo OX=3656 GN=LOC103484466 PE=4 SV=1	[more]
A0A6J1IU69	2.05e-78	84.85	GDSL esterase/lipase At5g45920 OS=Cucurbita maxima OX=3661 GN=LOC111478618 PE=3 ...	[more]
A0A6J1ED78	5.85e-78	84.09	GDSL esterase/lipase At5g45920 OS=Cucurbita moschata OX=3662 GN=LOC111433110 PE=...	[more]

BLAST of Cucsat.G2035 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G45920.1	1.2e-44	58.78	SGNH hydrolase-type esterase superfamily protein	[more]
AT5G62930.1	1.0e-30	48.46	SGNH hydrolase-type esterase superfamily protein	[more]
AT3G11210.1	6.0e-15	34.95	SGNH hydrolase-type esterase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036514	SGNH hydrolase superfamily	GENE3D	3.40.50.1110	SGNH hydrolase	coord: 2..235 e-value: 1.5E-73 score: 249.0
IPR013830	SGNH hydrolase-type esterase domain	PFAM	PF13472	Lipase_GDSL_2	coord: 7..197 e-value: 1.0E-27 score: 97.7
None	No IPR available	PANTHER	PTHR14209:SF26	ISOAMYL ACETATE-HYDROLYZING ESTERASE 1 HOMOLOG	coord: 1..238
None	No IPR available	CDD	cd01838	Isoamyl_acetate_hydrolase_like	coord: 4..206 e-value: 2.03527E-100 score: 288.382
None	No IPR available	SUPERFAMILY	52266	SGNH hydrolase	coord: 3..210
IPR045136	Isoamyl acetate-hydrolyzing esterase Iah1-like	PANTHER	PTHR14209	ISOAMYL ACETATE-HYDROLYZING ESTERASE 1	coord: 1..238

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G2035.T1	Cucsat.G2035.T1	mRNA
Cucsat.G2035.T3	Cucsat.G2035.T3	mRNA
Cucsat.G2035.T2	Cucsat.G2035.T2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016788	hydrolase activity, acting on ester bonds