Cucsat.G16538 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G16538
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	pre-mRNA-splicing factor CWC25 homolog
Location	ctg24: 263878 .. 266135 (+)
RNA-Seq Expression	Cucsat.G16538
Synteny	Cucsat.G16538

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AAATATTTGCGTAAATATTTTGATGGTAAGTTATATATTTTTTTAAAAAAACCTAATTTATAAATATAATATTAATCACATTTTAATTTTAAACCATAGTTTAAGAAAGAAAAATAATTGTTTTCATAATTTTGTTTTTAGATTTGAAAATCGGATTCTTCAACACAAAACCACACCGCCGGTTGCTGAAAATCCATCATCCGATCCCTAATCTGAAGCTCTGTTACAGACATGGCGTTGAAGTTCTTGAACAAGAAAGGATGGCATACTGGCAGTCTCCGGAACATAGAGAACGTATGGAAGGCGGAGCAAAAGCATGAGGCCGAACAGAAGAAGTTGGACGAGCTCCGCAAACAGATCCTCGAGGAGAAAGAACGCCAGGAGTTTCGTCTTCTTCAAGAACAAGCCGGCCTCGTTCCGTATGTTTCATCTTCTGTCTTTTTATTTTATTTCTGTTCTGTGAATTTCAAAATCTGATTCGATTTCTATGGGGATTGAAACCGTTCGTAGATTAGGGTTTGTGCCAAGGCGATGGCTAGGGTTTAATTAGTTCGATTGATTTGACCAATATGCAGGAAGCAGGAGAGATTGGATTTTCTTTATGAATCGGGTTTGGCAGTTGGGAAAGCTAGCAGTTCCGATGGATTTAAAAGCCTTGAAACCCTACCTAGTTCATCTACCGCCGCAGCTGCCACTGAACCATCTAGTTCCAAGGTACTGTTTCGTTTTTTCGTTGATTTGTGTGCTTTGGCTATCACTTTGTATGCTCAATTATGATGTTCTTGTGTTGTTTATGCAGGAAGCCGCTGTCCCTGGAGCTCTGTTTGAGGATAAGCCTCATTCTGCCAATGATACATGGAGGAAACTCCATTCTGATCCTTTGCTCATCATTCGACAGCGTGAGCAACAAGCTCTTGCTCGTGTTAAAAACAATCCTATTCAGATGGCTATGATTCGCAAAACTGTAAGTGTTTTTTAATCTCCTTATTGTTACAACATGGGCTGTGGAAATTTTGTGGAAACATCTTTCTTCCTTTCATTATTTTGTAGAGAGATTTTCTGAACTTCAACTGTTAGTTTGTTCATGGACACTTTACTTTGTGGTTGAGAAATCCGGTGGCCTATCTAAGTTCTAAGTTTTTCCTTTTGAGTTGATGTGATGGATTTCTCTTCATTTCTCTCACCCTTTTGATAGGAGCTAAAGATGAATCTTTGATCAGAAAGTTTCTTTCTATATATCTTCATGGCTAGTCACTTGTGGCAAAAGGACAAATGACGAACTGCCATGTTAAAGAATGTTCTTGTTGAGTTGTGATTGAATCTCAATTTAATTCACTATCGTATCATGTTGAAAGCTCTCTTGTTTGATTTGAAAAATAGATTTTGAGGGTTTGTTTTTTAATTATTTCTTCATATATATAATCATAGGTCGAAGTGGAAAAACACAAGGATAAAAATCCTGATGACAAGAGAGAACGGAAGAAGCACCGCCATAGTAAGTCTAAGCGCCATAAAGATTCTTCTCCAGAAAGAGATTATGATTCTGAAGATGTCAGCCCTGAAAGGCAGAGAAGGAAACATGATCATGACAAGAGTTCAAGACATGATGGCCATTCTCATTCAGAAGATAGAAGAAGCAAAGCAGAAACTAAGAACGAGAGGGACCGTGATAGAGGCTCAAAATATGCTGCACGAACAAGCTATGATCAGTCAGATCGAAAGACATTCAAAAGCAATCCTCATGATTCTGCAGCAGATAGATATCATGACAGATCAAAGAGAGATAGAGATTCTTATGCTAACAATGATAGGGACAGCAGAAGAGTGGGGGAACCGAGATATTATGAATCCAATGCTTCTGAAACTCCTAACGAGTCACGCCACAGGCATCGCAGGCCTACCACAAAGCTTTCAGAAGAAGAGCGTGCTGCTAGACTGCGTGAAATGCAGCAAGATGCCGAGTTACATGAAGAGCAACGATTCAAGCGTTTGAAGAAGGCAGATGAGGATGATGCACTGGAAGCCAAACAAAATGCAGTGCCCTCGAGTCGAAATTTCTTGGACATGGCTCAGAAACGCATGTACGGTGCTGAGAAGGGAGGAAGCTCAACGATCGAGGAGAGTATTCGCCGTAGGACATATTATTCACAGGGTAAATCTCAGATTGAAGCCAATGCTTTTAGACGGTAATCAACATCACTTGTATACTTCTGCTTGTGGTTAGTGTATAATTTGCCTTTTGAAGAATTGCTTTTTCATATATCAATGTGATCTGATCAGATTTGGGGTTTGTGATGGTTAGATTCATATATGGTTTGTTTTAATTAAATAGTTGTATTTTCTATTGATATTGATATATATTATCAACTTGTCTAACATTTCTTCTTCCTTATTATGTGAAAATTGTGACCTTGTTTGCTC

Coding sequence (CDS)

ATGGCGTTGAAGTTCTTGAACAAGAAAGGATGGCATACTGGCAGTCTCCGGAACATAGAGAACGTATGGAAGGCGGAGCAAAAGCATGAGGCCGAACAGAAGAAGTTGGACGAGCTCCGCAAACAGATCCTCGAGGAGAAAGAACGCCAGGAGTTTCGTCTTCTTCAAGAACAAGCCGGCCTCGTTCCGAAGCAGGAGAGATTGGATTTTCTTTATGAATCGGGTTTGGCAGTTGGGAAAGCTAGCAGTTCCGATGGATTTAAAAGCCTTGAAACCCTACCTAGTTCATCTACCGCCGCAGCTGCCACTGAACCATCTAGTTCCAAGGAAGCCGCTGTCCCTGGAGCTCTGTTTGAGGATAAGCCTCATTCTGCCAATGATACATGGAGGAAACTCCATTCTGATCCTTTGCTCATCATTCGACAGCGTGAGCAACAAGCTCTTGCTCGTGTTAAAAACAATCCTATTCAGATGGCTATGATTCGCAAAACTGTCGAAGTGGAAAAACACAAGGATAAAAATCCTGATGACAAGAGAGAACGGAAGAAGCACCGCCATAGTAAGTCTAAGCGCCATAAAGATTCTTCTCCAGAAAGAGATTATGATTCTGAAGATGTCAGCCCTGAAAGGCAGAGAAGGAAACATGATCATGACAAGAGTTCAAGACATGATGGCCATTCTCATTCAGAAGATAGAAGAAGCAAAGCAGAAACTAAGAACGAGAGGGACCGTGATAGAGGCTCAAAATATGCTGCACGAACAAGCTATGATCAGTCAGATCGAAAGACATTCAAAAGCAATCCTCATGATTCTGCAGCAGATAGATATCATGACAGATCAAAGAGAGATAGAGATTCTTATGCTAACAATGATAGGGACAGCAGAAGAGTGGGGGAACCGAGATATTATGAATCCAATGCTTCTGAAACTCCTAACGAGTCACGCCACAGGCATCGCAGGCCTACCACAAAGCTTTCAGAAGAAGAGCGTGCTGCTAGACTGCGTGAAATGCAGCAAGATGCCGAGTTACATGAAGAGCAACGATTCAAGCGTTTGAAGAAGGCAGATGAGGATGATGCACTGGAAGCCAAACAAAATGCAGTGCCCTCGAGTCGAAATTTCTTGGACATGGCTCAGAAACGCATGTACGGTGCTGAGAAGGGAGGAAGCTCAACGATCGAGGAGAGTATTCGCCGTAGGACATATTATTCACAGGGTAAATCTCAGATTGAAGCCAATGCTTTTAGACGGTAA

Protein sequence

MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAGLVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAVPGALFEDKPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRERKKHRHSKSKRHKDSSPERDYDSEDVSPERQRRKHDHDKSSRHDGHSHSEDRRSKAETKNERDRDRGSKYAARTSYDQSDRKTFKSNPHDSAADRYHDRSKRDRDSYANNDRDSRRVGEPRYYESNASETPNESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQRFKRLKKADEDDALEAKQNAVPSSRNFLDMAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSQIEANAFRR

Homology

[Close]

BLAST of Cucsat.G16538 vs. ExPASy Swiss-Prot
Match: Q9NXE8 (Pre-mRNA-splicing factor CWC25 homolog OS=Homo sapiens OX=9606 GN=CWC25 PE=1 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 6.7e-18
Identity = 62/172 (36.05%), Postives = 100/172 (58.14%), Query Frame = 0


Query: 8   KKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAGLV-PKQE 67
           KK WH  +LRN+E VWKAEQKHEAE+KK++EL++++ EE+ R+E +   E  G V  K+E
Sbjct: 9   KKSWHPQTLRNVEKVWKAEQKHEAERKKIEELQRELREERAREEMQRYAEDVGAVKKKEE 68

Query: 68  RLDFLYE--SGLA------VGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAVPGALF 127
           +LD++Y+   G+       +G+      F+ +E   +           SS+   +PG++F
Sbjct: 69  KLDWMYQGPGGMVNRDEYLLGRPIDKYVFEKMEEKEAG---------CSSETGLLPGSIF 128

Query: 128 EDKPHSAN---DTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVSV 168
              P  AN   D   K+  DPL IIR++E++    V NNP++M  I++ + +
Sbjct: 129 --APSGANSLLDMASKIREDPLFIIRKKEEEKKREVLNNPVKMKKIKELLQM 169

[Close]

BLAST of Cucsat.G16538 vs. ExPASy Swiss-Prot
Match: Q9DBF7 (Pre-mRNA-splicing factor CWC25 homolog OS=Mus musculus OX=10090 GN=Cwc25 PE=2 SV=2)

HSP 1 Score: 92.0 bits (227), Expect = 6.7e-18
Identity = 62/172 (36.05%), Postives = 100/172 (58.14%), Query Frame = 0


Query: 8   KKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAGLV-PKQE 67
           KK WH  +LRN+E VWKAEQKHEAE+KK++EL++++ EE+ R+E +   E  G V  K+E
Sbjct: 9   KKSWHPQTLRNVEKVWKAEQKHEAERKKIEELQRELREERAREEMQRYAEDVGAVKKKEE 68

Query: 68  RLDFLYE--SGLA------VGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAVPGALF 127
           +LD++Y+   G+       +G+      F+ +E   +           SS+   +PG++F
Sbjct: 69  KLDWMYQGPGGMVNRDEYLLGRPIDKYVFEKMEEREAG---------CSSETGLLPGSIF 128

Query: 128 EDKPHSAN---DTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVSV 168
              P  AN   D   K+  DPL IIR++E++    V NNP++M  I++ + +
Sbjct: 129 --APSGANSLLDMASKIREDPLFIIRKKEEEKKREVLNNPVKMKKIKELLQM 169

[Close]

BLAST of Cucsat.G16538 vs. ExPASy Swiss-Prot
Match: Q5B0I1 (Pre-mRNA-splicing factor cwc25 OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=cwc25 PE=3 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 4.3e-09
Identity = 52/153 (33.99%), Postives = 79/153 (51.63%), Query Frame = 0


Query: 8   KKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAGLVPKQER 67
           KK WH   LRN E VW  E++   E+K++D+L+++  EE++ QE + LQE AG   + +R
Sbjct: 8   KKSWHPSLLRNQERVWAEEKRALEERKRIDQLKRERDEERQIQELQRLQESAGGTRQLQR 67

Query: 68  LDFLYE-------------SGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAVP 127
           +D++Y+              G  +GK    DG              AAT P ++  A +P
Sbjct: 68  VDWMYQEPSSASGHYAEEMEGYLLGK-RRIDGILLKNDESKKLEKGAATGPGAA--AGLP 127

Query: 128 GALFEDKPHSANDTWRKLHSDPLLIIRQREQQA 148
             +     H+  DT  K+ +DPLL IR+REQ A
Sbjct: 128 PVV-----HNTRDTMAKVMADPLLEIRKREQAA 152

[Close]

BLAST of Cucsat.G16538 vs. ExPASy Swiss-Prot
Match: Q4P6I3 (Pre-mRNA-splicing factor CWC25 OS=Ustilago maydis (strain 521 / FGSC 9021) OX=237631 GN=CWC25 PE=3 SV=2)

HSP 1 Score: 62.4 bits (150), Expect = 5.7e-09
Identity = 47/166 (28.31%), Postives = 86/166 (51.81%), Query Frame = 0


Query: 8   KKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAGLVPKQER 67
           KK WH     N E VWK E++   E+KKL+ELR++  +E+E Q+ + LQE+AG   + +R
Sbjct: 9   KKSWHPLLQVNQERVWKREKEALEERKKLEELRREREQEREMQQLQRLQEEAGGKKRIDR 68

Query: 68  LDFLYESGLAVGKASSSD------GFKSLETLPSSSTAAAATEPSSSKEAAVPGALFEDK 127
           +D++Y +    G  S+++      G K ++ L         ++ +     ++  A     
Sbjct: 69  VDWMYATPATTGSGSAAEMEDYLLGKKRVDKLLQGEENKEMSKTTQKSLVSLQNA----- 128

Query: 128 PHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVSV 168
            +SA D   K+  DP+L I+Q+EQ A   +  +P ++  ++    +
Sbjct: 129 -NSARDLAAKIREDPMLAIKQQEQAAYEALLRDPTRLRQLKMQAGI 168

[Close]

BLAST of Cucsat.G16538 vs. ExPASy Swiss-Prot
Match: P0CM99 (Pre-mRNA-splicing factor CWC25 OS=Cryptococcus neoformans var. neoformans serotype D (strain B-3501A) OX=283643 GN=CWC25 PE=3 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 2.8e-08
Identity = 51/152 (33.55%), Postives = 76/152 (50.00%), Query Frame = 0


Query: 8   KKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAGLVPKQER 67
           KK WH   L N E VWKAE+    E+K L +LRK+  EE++ +E   LQE +    + E+
Sbjct: 9   KKSWHPVLLVNQERVWKAEKVANEEKKMLAQLRKEREEERQLEELHRLQEASTGKKRVEK 68

Query: 68  LDFLYES----GLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAVPGALFEDKPH 127
           LD++Y +    G A+G A   +       L          +   +  AA    +     +
Sbjct: 69  LDWMYAAPSTEGGALGGARIGERDMEEYLLGKKRVDEVLGQGDKNIGAASREFIALQNAN 128

Query: 128 SANDTWRKLHSDPLLIIRQREQQALARVKNNP 156
           +A DT  K+  DPLL I+++EQ ALA + N P
Sbjct: 129 TARDTAAKIREDPLLAIKKQEQAALAALMNRP 160

[Close]

BLAST of Cucsat.G16538 vs. NCBI nr
Match: XP_004146897.1 (pre-mRNA-splicing factor CWC25 homolog [Cucumis sativus] >KGN53175.1 hypothetical protein Csa_014942 [Cucumis sativus])

HSP 1 Score: 309 bits (791), Expect = 6.18e-102
Identity = 166/167 (99.40%), Postives = 166/167 (99.40%), Query Frame = 0


Query: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG 60
           MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG
Sbjct: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG 60

Query: 61  LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAVPGALFED 120
           LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAVPGALFED
Sbjct: 61  LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAVPGALFED 120

Query: 121 KPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVSV 167
           KPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTV V
Sbjct: 121 KPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEV 167

[Close]

BLAST of Cucsat.G16538 vs. NCBI nr
Match: XP_008453851.1 (PREDICTED: pre-mRNA-splicing factor CWC25 homolog [Cucumis melo] >KAA0044681.1 pre-mRNA-splicing factor CWC25-like protein [Cucumis melo var. makuwa] >TYK16902.1 pre-mRNA-splicing factor CWC25-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 305 bits (782), Expect = 1.51e-100
Identity = 164/167 (98.20%), Postives = 164/167 (98.20%), Query Frame = 0


Query: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG 60
           MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG
Sbjct: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG 60

Query: 61  LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAVPGALFED 120
           LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAA PGALFED
Sbjct: 61  LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAAPGALFED 120

Query: 121 KPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVSV 167
           KPHSAND WRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTV V
Sbjct: 121 KPHSANDAWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEV 167

[Close]

BLAST of Cucsat.G16538 vs. NCBI nr
Match: XP_038896474.1 (pre-mRNA-splicing factor CWC25 homolog [Benincasa hispida])

HSP 1 Score: 305 bits (780), Expect = 2.77e-100
Identity = 164/167 (98.20%), Postives = 164/167 (98.20%), Query Frame = 0


Query: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG 60
           MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQI EEKERQEFRLLQEQAG
Sbjct: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAG 60

Query: 61  LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAVPGALFED 120
           LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAVPGALFED
Sbjct: 61  LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAVPGALFED 120

Query: 121 KPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVSV 167
           KPHSAND WRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTV V
Sbjct: 121 KPHSANDAWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEV 167

[Close]

BLAST of Cucsat.G16538 vs. NCBI nr
Match: KAG6576831.1 (Pre-mRNA-splicing factor CWC25-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 292 bits (747), Expect = 3.53e-96
Identity = 158/168 (94.05%), Postives = 162/168 (96.43%), Query Frame = 0


Query: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG 60
           MALKFLNKKGWHTG+LRNIENVWKAEQKHEAEQKKLDELRKQI EEKERQEFRLLQEQAG
Sbjct: 1   MALKFLNKKGWHTGNLRNIENVWKAEQKHEAEQKKLDELRKQIHEEKERQEFRLLQEQAG 60

Query: 61  LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSS-STAAAATEPSSSKEAAVPGALFE 120
           LVPKQERLDFLYESGLAVGK S+SDGFKSLETLP++ STAAAATEPSSSKEAA PGALFE
Sbjct: 61  LVPKQERLDFLYESGLAVGKGSNSDGFKSLETLPTTTSTAAAATEPSSSKEAAAPGALFE 120

Query: 121 DKPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVSV 167
           DKPHSAND WRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTV V
Sbjct: 121 DKPHSANDAWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEV 168

[Close]

BLAST of Cucsat.G16538 vs. NCBI nr
Match: XP_023551879.1 (LOW QUALITY PROTEIN: pre-mRNA-splicing factor CWC25 homolog, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 291 bits (746), Expect = 4.11e-95
Identity = 158/167 (94.61%), Postives = 162/167 (97.01%), Query Frame = 0


Query: 2   ALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAGL 61
           ALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQI EEKERQEFRLLQEQAGL
Sbjct: 7   ALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIHEEKERQEFRLLQEQAGL 66

Query: 62  VPKQERLDFLYESGLAVGKASSSDGFKSLETLPSS-STAAAATEPSSSKEAAVPGALFED 121
           VPKQERLDFLYESGLAVGK S+SDGFKSLETLP++ STAAAATEPSSSKEAA PGALFED
Sbjct: 67  VPKQERLDFLYESGLAVGKGSNSDGFKSLETLPTTTSTAAAATEPSSSKEAAAPGALFED 126

Query: 122 KPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVSV 167
           KPHSAND+WRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTV V
Sbjct: 127 KPHSANDSWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEV 173

[Close]

BLAST of Cucsat.G16538 vs. ExPASy TrEMBL
Match: A0A0A0KU62 (Cir_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G025080 PE=3 SV=1)

HSP 1 Score: 309 bits (791), Expect = 2.99e-102
Identity = 166/167 (99.40%), Postives = 166/167 (99.40%), Query Frame = 0


Query: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG 60
           MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG
Sbjct: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG 60

Query: 61  LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAVPGALFED 120
           LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAVPGALFED
Sbjct: 61  LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAVPGALFED 120

Query: 121 KPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVSV 167
           KPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTV V
Sbjct: 121 KPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEV 167

[Close]

BLAST of Cucsat.G16538 vs. ExPASy TrEMBL
Match: A0A5A7TSZ0 (Pre-mRNA-splicing factor CWC25-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G00360 PE=3 SV=1)

HSP 1 Score: 305 bits (782), Expect = 7.33e-101
Identity = 164/167 (98.20%), Postives = 164/167 (98.20%), Query Frame = 0


Query: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG 60
           MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG
Sbjct: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG 60

Query: 61  LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAVPGALFED 120
           LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAA PGALFED
Sbjct: 61  LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAAPGALFED 120

Query: 121 KPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVSV 167
           KPHSAND WRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTV V
Sbjct: 121 KPHSANDAWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEV 167

[Close]

BLAST of Cucsat.G16538 vs. ExPASy TrEMBL
Match: A0A1S3BY15 (pre-mRNA-splicing factor CWC25 homolog OS=Cucumis melo OX=3656 GN=LOC103494454 PE=3 SV=1)

HSP 1 Score: 305 bits (782), Expect = 7.33e-101
Identity = 164/167 (98.20%), Postives = 164/167 (98.20%), Query Frame = 0


Query: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG 60
           MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG
Sbjct: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG 60

Query: 61  LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAVPGALFED 120
           LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAA PGALFED
Sbjct: 61  LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAAPGALFED 120

Query: 121 KPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVSV 167
           KPHSAND WRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTV V
Sbjct: 121 KPHSANDAWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEV 167

[Close]

BLAST of Cucsat.G16538 vs. ExPASy TrEMBL
Match: A0A6J1CHZ1 (pre-mRNA-splicing factor CWC25 homolog OS=Momordica charantia OX=3673 GN=LOC111011671 PE=3 SV=1)

HSP 1 Score: 291 bits (744), Expect = 3.44e-95
Identity = 155/167 (92.81%), Postives = 159/167 (95.21%), Query Frame = 0


Query: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG 60
           MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQI EEKERQEFRLLQEQAG
Sbjct: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAG 60

Query: 61  LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAVPGALFED 120
           LVPKQERLDFLYESGLAVGK SSSDGFKSLETLPS+S   A TEPSSSKEAA PGALFE+
Sbjct: 61  LVPKQERLDFLYESGLAVGKGSSSDGFKSLETLPSTSAIVATTEPSSSKEAAAPGALFEN 120

Query: 121 KPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVSV 167
           KPHSAND+WRKLHSDPLLIIRQREQ+ALARVKNNPIQMAMIRKTV V
Sbjct: 121 KPHSANDSWRKLHSDPLLIIRQREQEALARVKNNPIQMAMIRKTVKV 167

[Close]

BLAST of Cucsat.G16538 vs. ExPASy TrEMBL
Match: A0A6J1E963 (LOW QUALITY PROTEIN: pre-mRNA-splicing factor CWC25 homolog OS=Cucurbita moschata OX=3662 GN=LOC111430542 PE=3 SV=1)

HSP 1 Score: 288 bits (738), Expect = 3.04e-94
Identity = 157/168 (93.45%), Postives = 160/168 (95.24%), Query Frame = 0


Query: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG 60
           MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQI EEKERQEFRLLQEQAG
Sbjct: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIHEEKERQEFRLLQEQAG 60

Query: 61  LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSS-STAAAATEPSSSKEAAVPGALFE 120
           LVPKQERLDFLYESGLAVGK S+SDGFKSLETLP++ STAAAA EPSSSKEAA PGALFE
Sbjct: 61  LVPKQERLDFLYESGLAVGKGSNSDGFKSLETLPTTTSTAAAAPEPSSSKEAAAPGALFE 120

Query: 121 DKPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVSV 167
           DKPHSAND WRKLHSDPLLIIRQREQ ALARVKNNPIQMAMIRKTV V
Sbjct: 121 DKPHSANDAWRKLHSDPLLIIRQREQXALARVKNNPIQMAMIRKTVEV 168

The following BLAST results are available for this feature:

BLAST of Cucsat.G16538 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9NXE8	6.7e-18	36.05	Pre-mRNA-splicing factor CWC25 homolog OS=Homo sapiens OX=9606 GN=CWC25 PE=1 SV=...	[more]
Q9DBF7	6.7e-18	36.05	Pre-mRNA-splicing factor CWC25 homolog OS=Mus musculus OX=10090 GN=Cwc25 PE=2 SV...	[more]
Q5B0I1	4.3e-09	33.99	Pre-mRNA-splicing factor cwc25 OS=Emericella nidulans (strain FGSC A4 / ATCC 381...	[more]
Q4P6I3	5.7e-09	28.31	Pre-mRNA-splicing factor CWC25 OS=Ustilago maydis (strain 521 / FGSC 9021) OX=23...	[more]
P0CM99	2.8e-08	33.55	Pre-mRNA-splicing factor CWC25 OS=Cryptococcus neoformans var. neoformans seroty...	[more]

BLAST of Cucsat.G16538 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004146897.1	6.18e-102	99.40	pre-mRNA-splicing factor CWC25 homolog [Cucumis sativus] >KGN53175.1 hypothetica...	[more]
XP_008453851.1	1.51e-100	98.20	PREDICTED: pre-mRNA-splicing factor CWC25 homolog [Cucumis melo] >KAA0044681.1 p...	[more]
XP_038896474.1	2.77e-100	98.20	pre-mRNA-splicing factor CWC25 homolog [Benincasa hispida]	[more]
KAG6576831.1	3.53e-96	94.05	Pre-mRNA-splicing factor CWC25-like protein, partial [Cucurbita argyrosperma sub...	[more]
XP_023551879.1	4.11e-95	94.61	LOW QUALITY PROTEIN: pre-mRNA-splicing factor CWC25 homolog, partial [Cucurbita ...	[more]

BLAST of Cucsat.G16538 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KU62	2.99e-102	99.40	Cir_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G025080 PE=3 ...	[more]
A0A5A7TSZ0	7.33e-101	98.20	Pre-mRNA-splicing factor CWC25-like protein OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A1S3BY15	7.33e-101	98.20	pre-mRNA-splicing factor CWC25 homolog OS=Cucumis melo OX=3656 GN=LOC103494454 P...	[more]
A0A6J1CHZ1	3.44e-95	92.81	pre-mRNA-splicing factor CWC25 homolog OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1E963	3.04e-94	93.45	LOW QUALITY PROTEIN: pre-mRNA-splicing factor CWC25 homolog OS=Cucurbita moschat...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 22..49
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 163..179
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 85..105
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 150..329
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 354..374
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 193..302
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 80..129
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 310..329
None	No IPR available	PANTHER	PTHR16196	CELL CYCLE CONTROL PROTEIN CWF25	coord: 1..417
IPR019339	CBF1-interacting co-repressor CIR, N-terminal domain	SMART	SM01083	Cir_N_3	coord: 10..46 e-value: 2.4E-13 score: 60.3
IPR019339	CBF1-interacting co-repressor CIR, N-terminal domain	PFAM	PF10197	Cir_N	coord: 11..46 e-value: 9.8E-15 score: 54.5
IPR022209	Pre-mRNA splicing factor	PFAM	PF12542	CWC25	coord: 64..155 e-value: 5.3E-12 score: 46.5

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G16538.T2	Cucsat.G16538.T2	mRNA
Cucsat.G16538.T1	Cucsat.G16538.T1	mRNA
Cucsat.G16538.T3	Cucsat.G16538.T3	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000398	mRNA splicing, via spliceosome
cellular_component	GO:0005684	U2-type spliceosomal complex