Cucsat.G14528 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G14528
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Exonuclease domain-containing protein
Location	ctg1869: 723474 .. 726950 (+)
RNA-Seq Expression	Cucsat.G14528
Synteny	Cucsat.G14528

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CCTGACGAAGCAAACTCTTAAAACCCCCCATGGCGGCCGTTAACGTTATGTTGCTTTCAATTCTTCGCGCCTTCTTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCAATCTCATACCAATCTGCTAGTCTTATTTCTCTCTTCTCCCATTGAAATTTCTCTTAAACAATCATGGGTCCGACGGAGGATCGTTCCGAGATTGCCTTCTTTGACGTCGAGACCACCGTTCCCACCCGCCAAGGTCAGAAATTTTCTATTTTGGAATTTGGAGCCATTCTGGTTTGCCCTAAGAAGTTGGTTGAGTTGGAAAGTTATTCCACTCTCGTAAAACCCTCTGATCTCTCCCTCATTTCCTCCTTATCGGTGCGATGCAATGGCATTACTAGAGATGCTGTCATTTCTTCCCCTACTTTCGCCCAAATTGCTGATAGGGTTTACGATATACTTCACGGTACTCTCTATTTTTGATGTATTATATATGCATGTGGGATGAGTATTAAATTGATGTGGGAGTTGAATTGGGTGTCTTCTGATGGAACTGAGAGATGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTCAAAGGAGAGATTTTGGCTCAGAATTGTTCGTGCTTCTTTTACTTTAAATTTTAGTGTATGTCTGTGTTTGAGTTTCCTCTGGTGCGATTCTGTTTCCTGTAGGGCGGATATGGGCGGGTCATAACATACTTAGATTTGATTGTGCACGAATCAGGGAGGCTTTTGCAGAAATTGGTGTGCCAGCACCTGAGCCAAAAGGTACAATTGATTCATTGGCATTGTTGACTCAAAGGTTTGGAAGGAGAGCTGGGGACATGAAGGTAATGACTGAATCCTTTCTCTGTTGCATGCTTGCTATGGCTTTTGTTCATTCAGGTGATCTTGAGAAATCTATACACTAGTTTCCATTAGATGGTGCCTTCGTCAAAAATTTATCAAGAGTTTTCAGCATTCAGAATTCGGATTTATCTCTCTAATTATGAGACTTAAATGATGTTAACAATTGGCACTAGGATAGGATTACATTACAAATATATAAGGTTTTACTAATTTAGTATCTAACTATTAAATATTATGCGAACGAGGGGTTGGGAAAACCACGTTTTGGGGAAGGCCTCTTGTAATGATATTTTCCTTGTTTTGAGTTTCCCTGTTAATGCAACCCCATTGTGTTGGCTTTACATAATATATCTTCATGCATGCTCTGACTTTGGGTTCTGGGTTGTAGAAGCTATGGTAACTCTTTATTTATTTATTTATTTTAATATATATTCCTTTCCTCCCTGTAGATGGCCACCCTTGCTTCCTATTTCGGGATAGGACAACAAACACACAGGCAACTCTTCAAACTTGCTACTAATTTTTAGCTTAAACTAAAGGAACGTTTTCTAGTTATCTGACTTTGTCTTTGTAATTTTCCAGGAGTTTGGATGATGTTAGAATGAATCTCGAAGTTCTAAAATATTGTGCAACCGTCTTGTTTCTGGTAATATATAAATTTAAGATGTTGAACATAACTAATATGTACATGTGAACTTGTAACTATGTGAATAACTAATTAATGGCTTGCAGTTTAGGCCATTGCCAATATTATATACATGGAATTTTTGTTGCATTATTTGAATATTGACCACTTGGATGTCAGCGCATGATAACCGCGATTCTTTCTGAATTTAAATCATGATAGGCTCCAAATGGTTCGTTGTCAGTTTTTGCATCAATTGACATTACTTGTCTCCATTTTGCCTTCCTAGCACCTGTCATGTGGAGACATTCTTGTAGTAGTTTGTCTTTCAGTTTATTTTTAACGTTTTAACTTTTTCTAACATCAACGTAGTTGCATTAGTTTTGCTCTTATAGTGACCATTTTATATAAGAAGGGGATGGTGGTTTAAACTGTTGTCTGGCAACAGGAATCAAGTCTACCCGAAGTTTTCCCAGAGAACAGTTGGGTTTCTCCAAATGCTGTTACTAGGCGTCGTGCTGCAAAGTCGTCTCCACAGGGGAATAGTTCAAACAATAATTCTCACGCATCAAGTTCAAATATTGGTGGTAATCCCATATCTCTACCAGTTCAACAAGGGGAAACTCATCCGATACTTTCTCTTGTAACTATCTGTTCTGAGGACAGAGCCTCAATTCTGGCTGAACCTAGTGCAACTGAATCAGATTCTTTTAATATGCACACTACCAGCGACCAAATAACAGGTGCAACACTCGAAACAGATATCAACATGGAAGAACATGCCACAGTATCTACCGAAGCATCTCCCTCAGAGGATACTTCCACAAGTCTTTGCACTACTAAATTTTTGGAGCCAGATCAGGTGTCTGTTTCGTTCATCACTGCATCTTTCGTTCCATTTTTTCGTGGGAGCCAAAGAATACAGTTATGGCACAAAGATGACTGTTTACAGCTTCTATGTAATAATCTGAGAGTTCGGTTTGGCATAAGCACGAAATTCACTGACTACGCTGGCCGTCCGAGGTTGAGTTTTGTTGTTGATGTACCACCAAATTTATGCACGGTTCTTGAAGCATCTGATGGAGTGGCTCAGAGATTATTTTCTGATTCTGGCAGCGGTTCTGAATGGAGGCCTGCAGTGACGAGAAAGAATGGTTATTTCAACTACCCGACAATGAGATTACAGTAAGTCATACTCCCATGTTCCATCTGATCCTTTGATATGTAAACTGACTACTATCATCGACAAACATTTTGATGAATTGAATTATCAAATGAGGAAAAGAAACAGAAAATAACAGAGAATCTAGTCCAACATATGATCTGCACGCTTGCTTGCTTCCTTCAAATTTTCATTTCTTGCCGTCTCAATCCATTCATAATTTCCTCTTGTTGGTCACTTCCTAATTGATATTAACTTTGTTTTTTCACAGCATTCCAACTGCAGTAAGTGGAGATGTGGCAAATTATGCTACAGAAATGCACCAAAAAGAAGCATCAGGCGCTGTTCAAAGGTTAATATTCAGTAAATTTGATGCTGCAGAGCTTGATAGCTTGATCAAACCTGGAGCCATTTTAGATGCATTCATTTCCTTGGATACATATGACTATCAACAGAGTGCAGGCATTAGATTGGTAGCAAAAAAGTTGATTATCCGTTCCTAGTAATGGGAGGTTTATTTGTCCATTACCGAAGTAGCTCTGATGTTATATTATACTTAGTTCACTGTCTAACATTCAAATTTCCAATAAGTCCCTTCTCATCATTTTAAGAAAAAACAACACTTCTCCGGATTGCTTGATTGTGGATAAAATGCTTTAAAGAAACCTTAATTCAACCATAATTGATATAACATTTCTCGATCTATATCATATGATTCTTTTGCTCACAACTCCCAATTATGTGCCCTCGATATACTTATTGACTTGGTTAAAATTTGAGGATAAAATATATAGATTTTTTACTTTTTGGGTGGAG

Coding sequence (CDS)

ATGGGTCCGACGGAGGATCGTTCCGAGATTGCCTTCTTTGACGTCGAGACCACCGTTCCCACCCGCCAAGGTCAGAAATTTTCTATTTTGGAATTTGGAGCCATTCTGGTTTGCCCTAAGAAGTTGGTTGAGTTGGAAAGTTATTCCACTCTCGTAAAACCCTCTGATCTCTCCCTCATTTCCTCCTTATCGGTGCGATGCAATGGCATTACTAGAGATGCTGTCATTTCTTCCCCTACTTTCGCCCAAATTGCTGATAGGGTTTACGATATACTTCACGGGCGGATATGGGCGGGTCATAACATACTTAGATTTGATTGTGCACGAATCAGGGAGGCTTTTGCAGAAATTGGTGTGCCAGCACCTGAGCCAAAAGGTACAATTGATTCATTGGCATTGTTGACTCAAAGGTTTGGAAGGAGAGCTGGGGACATGAAGATGGCCACCCTTGCTTCCTATTTCGGGATAGGACAACAAACACACAGGAGTTTGGATGATGTTAGAATGAATCTCGAAGTTCTAAAATATTGTGCAACCGTCTTGTTTCTGGAATCAAGTCTACCCGAAGTTTTCCCAGAGAACAGTTGGGTTTCTCCAAATGCTGTTACTAGGCGTCGTGCTGCAAAGTCGTCTCCACAGGGGAATAGTTCAAACAATAATTCTCACGCATCAAGTTCAAATATTGGTGGTAATCCCATATCTCTACCAGTTCAACAAGGGGAAACTCATCCGATACTTTCTCTTGTAACTATCTGTTCTGAGGACAGAGCCTCAATTCTGGCTGAACCTAGTGCAACTGAATCAGATTCTTTTAATATGCACACTACCAGCGACCAAATAACAGGTGCAACACTCGAAACAGATATCAACATGGAAGAACATGCCACAGTATCTACCGAAGCATCTCCCTCAGAGGATACTTCCACAAGTCTTTGCACTACTAAATTTTTGGAGCCAGATCAGGTGTCTGTTTCGTTCATCACTGCATCTTTCGTTCCATTTTTTCGTGGGAGCCAAAGAATACAGTTATGGCACAAAGATGACTGTTTACAGCTTCTATGTAATAATCTGAGAGTTCGGTTTGGCATAAGCACGAAATTCACTGACTACGCTGGCCGTCCGAGGTTGAGTTTTGTTGTTGATGTACCACCAAATTTATGCACGGTTCTTGAAGCATCTGATGGAGTGGCTCAGAGATTATTTTCTGATTCTGGCAGCGGTTCTGAATGGAGGCCTGCAGTGACGAGAAAGAATGGTTATTTCAACTACCCGACAATGAGATTACACATTCCAACTGCAGTAAGTGGAGATGTGGCAAATTATGCTACAGAAATGCACCAAAAAGAAGCATCAGGCGCTGTTCAAAGGTTAATATTCAGTAAATTTGATGCTGCAGAGCTTGATAGCTTGATCAAACCTGGAGCCATTTTAGATGCATTCATTTCCTTGGATACATATGACTATCAACAGAGTGCAGGCATTAGATTGGTAGCAAAAAAGTTGATTATCCGTTCCTAG

Protein sequence

MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLISSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVPAPEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATVLFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGNSSNNNSHASSSNIGGNPISLPVQQGETHPILSLVTICSEDRASILAEPSATESDSFNMHTTSDQITGATLETDINMEEHATVSTEASPSEDTSTSLCTTKFLEPDQVSVSFITASFVPFFRGSQRIQLWHKDDCLQLLCNNLRVRFGISTKFTDYAGRPRLSFVVDVPPNLCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKNGYFNYPTMRLHIPTAVSGDVANYATEMHQKEASGAVQRLIFSKFDAAELDSLIKPGAILDAFISLDTYDYQQSAGIRLVAKKLIIRS

Homology

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BLAST of Cucsat.G14528 vs. ExPASy Swiss-Prot
Match: Q9FLR0 (Protein NEN1 OS=Arabidopsis thaliana OX=3702 GN=NEN1 PE=2 SV=1)

HSP 1 Score: 535.8 bits (1379), Expect = 5.3e-151
Identity = 291/504 (57.74%), Postives = 355/504 (70.44%), Query Frame = 0


Query: 5   EDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLISSLS 64
           EDRSEIAFFDVETTVP R GQ+F+ILEFG+ILVCPKKL EL SY+TLV+P+DLSLISSLS
Sbjct: 4   EDRSEIAFFDVETTVPKR-GQRFAILEFGSILVCPKKLTELRSYTTLVQPADLSLISSLS 63

Query: 65  VRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVPAPEP 124
           VRCNGI RD V+ +P FA IAD VYDILHGRIWAGHNILRFDCARIREAFAEIG   PEP
Sbjct: 64  VRCNGIKRDDVVLAPLFADIADTVYDILHGRIWAGHNILRFDCARIREAFAEIGRQPPEP 123

Query: 125 KGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATVLFLE 184
           KG IDSL LLTQ+FGRRAGDMKMATLA YFG+G QTHRSLDDVRMNLEVLKYCATVLFLE
Sbjct: 124 KGAIDSLGLLTQKFGRRAGDMKMATLARYFGLGNQTHRSLDDVRMNLEVLKYCATVLFLE 183

Query: 185 SSLPEVFPENSWVSPNAVTRRRAAKSSPQGNSSNNNSHASSSNIGGNPISLPVQQGETHP 244
           SSLP    +NS VSP  ++ RR   +S +GN                             
Sbjct: 184 SSLPYAHVDNS-VSPETISSRRRIDASREGN----------------------------- 243

Query: 245 ILSLVTICSEDRASILAEPSATESDSFNMHTTSDQITGAT-LETDINMEEHATVSTEASP 304
                T+ +  R   ++E SA + D FNM    +++     +++DI MEE     ++   
Sbjct: 244 -----TVTTSVRLPSISENSAAQPDPFNMSVLRNEMASDNHIQSDILMEEEQIEPSDVVA 303

Query: 305 SEDTSTSLCTTKFLEPDQVSVSFITASFVPFFRGSQ--RIQLWHKDDCLQLLCNNLRVRF 364
           SE+TS       FL PD +S+S I A   PF+ GSQ  +++L H D  LQL C+ L++RF
Sbjct: 304 SENTSDH---EGFLTPDAMSLSNIKAMLFPFYPGSQMMKLKLLHGDSPLQLYCSYLKIRF 363

Query: 365 GISTKFTDYAGRPRLSFVVDVPPNLCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKNGYF 424
           G++ KF D  GR RL+FVVD+ P+L ++LEA D  AQ+L  DSGS SEW P V    G+ 
Sbjct: 364 GVNGKFLDNTGRRRLNFVVDLNPSLYSILEACDSNAQKLSVDSGSTSEWNPVVNPMKGFV 423

Query: 425 NYPTMRLHIPTAVSGDVANYATEMHQKEASGAVQRLIFSKFDAAELDSLIKPGAILDAFI 484
           NYP  R+HI T ++GD A YATE+HQ+E+SGA Q+LIFS  +  EL+SL+  G+++DAF+
Sbjct: 424 NYPNARIHIATEINGDEARYATEIHQRESSGATQKLIFSNPNNEELESLLTTGSVVDAFL 468

Query: 485 SLDTYDYQQSAGIRLVAKKLIIRS 506
           SL+ YDYQQ AGIRLVAKKL+I+S
Sbjct: 484 SLEPYDYQQKAGIRLVAKKLVIQS 468

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BLAST of Cucsat.G14528 vs. ExPASy Swiss-Prot
Match: Q0V842 (Protein NEN2 OS=Arabidopsis thaliana OX=3702 GN=NEN2 PE=2 SV=1)

HSP 1 Score: 516.2 bits (1328), Expect = 4.3e-145
Identity = 285/508 (56.10%), Postives = 353/508 (69.49%), Query Frame = 0


Query: 3   PTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLISS 62
           P EDRSEIAFFDVETT+P R GQ ++ILEFG+ILVCPKKLVEL++YS LV+P++L+LI+ 
Sbjct: 9   PAEDRSEIAFFDVETTIPFRVGQGYAILEFGSILVCPKKLVELKNYSVLVRPANLNLITP 68

Query: 63  LSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVPAP 122
            SV+CNGI R+ V S+ TFA IAD VYDILHGRIWAGHNIL+FDC RIREAFAEIG   P
Sbjct: 69  RSVKCNGIKREDVESALTFADIADTVYDILHGRIWAGHNILKFDCPRIREAFAEIGRDPP 128

Query: 123 EPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATVLF 182
           EPKGTIDSLALLTQRFGRRAGDMKMATLA+YFG+G QTHRSLDDVRMN EVLKYCATVLF
Sbjct: 129 EPKGTIDSLALLTQRFGRRAGDMKMATLATYFGLGNQTHRSLDDVRMNFEVLKYCATVLF 188

Query: 183 LESSLPEVFPENSWVSPNAVT---RRRAAKSSPQGNSSNNNSHASSSNIGGNPISLPVQQ 242
           LESSLP+   ENS  +    T   RRR  K SP  + ++  +  + + I           
Sbjct: 189 LESSLPDELIENSVTTTTPETSSRRRRTIKKSPLQSPTDQQTGENMTTI----------- 248

Query: 243 GETHPILSLVTICSEDRASILAEPSATESDSFNMHTTSDQITGATLETDINMEEHATVST 302
               PILS V+             +  ++D F+M T  ++I    L++D+ MEE     +
Sbjct: 249 ----PILSFVS------------SAEAQTDPFDMSTLRNEIAPEVLQSDVPMEEEQNQQS 308

Query: 303 EASPSEDTSTSLCTTKFLEPDQVSVSFITASFVPFFRGSQ--RIQLWHKDDCLQLLCNNL 362
           E   SE T        F+E D++SVS I A+ VP + GSQ  ++QL+  D  LQL C  L
Sbjct: 309 ETVASEGTGDQ---EGFMELDKISVSSIRATHVPLYDGSQTMKLQLFLGDRPLQLHCPRL 368

Query: 363 RVRFGISTKFTDYAGRPRLSFVVDVPPNLCTVLEASDGVAQRLFSDSGSGSEWRPAVTRK 422
           +VRFGI+ KF D AGR RL+FV+D+ P+LC VL+  D  AQ +  DSGSGS+W P V   
Sbjct: 369 KVRFGINGKFMDKAGRRRLNFVIDLYPSLCNVLQECDSAAQTISVDSGSGSDWNPLVIPM 428

Query: 423 NGYFNYPTMRLHIPTAVSGDVANYATEMHQKEASG--AVQRLIFSKFDAAELDSLIKPGA 482
            G+ N PT R+HIPT ++GD+  YA E+HQKE SG  A Q+LI S   A E++SL+ P  
Sbjct: 429 KGFLNCPTARIHIPTELNGDIDRYAAEIHQKEFSGATATQKLISSNPKAEEIESLLNPRT 486

Query: 483 ILDAFISLDTYDYQQSAGIRLVAKKLII 504
           +LDAF+SL+ YDYQQ AGIRLVA+KL+I
Sbjct: 489 VLDAFLSLEPYDYQQRAGIRLVARKLVI 486

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BLAST of Cucsat.G14528 vs. ExPASy Swiss-Prot
Match: Q9CA74 (Protein NEN3 OS=Arabidopsis thaliana OX=3702 GN=NEN3 PE=2 SV=1)

HSP 1 Score: 505.8 bits (1301), Expect = 5.8e-142
Identity = 274/503 (54.47%), Postives = 356/503 (70.78%), Query Frame = 0


Query: 5   EDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLISSLS 64
           ++RSEIAFFD+ET VPT+ G+ F+ILEFGAILVCP++L EL SYSTLV+P+DLSLIS+L+
Sbjct: 8   DERSEIAFFDLETAVPTKSGEPFAILEFGAILVCPRRLEELYSYSTLVRPTDLSLISTLT 67

Query: 65  VRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVPAPEP 124
            R +GITRD V+S+ TF++IAD+VYDILHGRIWAGHNI+RFDC RIREAFAEIG+  PEP
Sbjct: 68  KRRSGITRDGVLSAHTFSEIADKVYDILHGRIWAGHNIIRFDCVRIREAFAEIGLSPPEP 127

Query: 125 KGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATVLFLE 184
           K TIDSL+LL+Q+FG+RAGDMKMA+LA+YFG+G Q HRSLDDVRMNLEV+KYCATVLFLE
Sbjct: 128 KATIDSLSLLSQKFGKRAGDMKMASLATYFGLGDQAHRSLDDVRMNLEVVKYCATVLFLE 187

Query: 185 SSLPEVFPENSWVSPNAVTRRRA-AKSSPQGNSSNNNSHASSSNIGGNPISLPVQQGETH 244
           SS+P++  + SW SP    R R+  K    G   ++ S +SS     +  S+     + H
Sbjct: 188 SSVPDILTDMSWFSPRKSPRTRSNGKLVANGVRESSTSSSSSPKTDPSSSSVDATIVKNH 247

Query: 245 PILSLVTICSEDRASILAEPSATESDSFNMHTTSDQITGATLETDINMEEHATV--STEA 304
           PI+SL+T CSE  AS     S    D  ++ T   ++   TL+TD   EE  TV    E+
Sbjct: 248 PIVSLLTECSESDAS-----SYDIEDPIDITTLIGKLRIGTLQTDA-AEEAKTVRQQDES 307

Query: 305 SPSEDTSTSLCTTKFLEPDQVSVSFITASFVPFFRGSQRIQLWHKDDCLQLLCNNLRVRF 364
            PS D+        FL  ++VSVS I AS VPF+RGS R++L+H D  L L  ++L+VRF
Sbjct: 308 PPSPDSDAK--DESFLGVNEVSVSSIRASLVPFYRGSLRMKLFHNDTPLHLCWHSLKVRF 367

Query: 365 GISTKFTDYAGRPRLSFVVDVPPNLCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKNGYF 424
           GIS KF D+AGRP+L+ +VD P +LC +L+A D  A  L +DS + S+WRP V RK G+ 
Sbjct: 368 GISRKFVDHAGRPKLNIIVDAPLDLCKILDAVDAAAHNLPTDSSTNSDWRPTVIRKEGFA 427

Query: 425 NYPTMRLHIPTAVSGDVANYATEMHQKEAS-GAVQRLIFSKFDAAELDSLIKPGAILDAF 484
           NYPT RLHI +  +GD     T+++QKE   G  Q+L  S  +  +L+S + PG ++DAF
Sbjct: 428 NYPTARLHISSESNGDDTLCGTQVYQKEEPLGTNQKLDVSSDNLEKLESALLPGTLVDAF 487

Query: 485 ISLDTYDYQQSAGIRLVAKKLII 504
            SL+ Y YQQ AGIRL  KKL+I
Sbjct: 488 FSLEPYSYQQMAGIRLAVKKLVI 502

[Close]

BLAST of Cucsat.G14528 vs. ExPASy Swiss-Prot
Match: F4JJ23 (Protein NEN4 OS=Arabidopsis thaliana OX=3702 GN=NEN4 PE=2 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 5.0e-69
Identity = 126/199 (63.32%), Postives = 158/199 (79.40%), Query Frame = 0


Query: 8   SEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLISSLSVRC 67
           +EI FFD+ET VP + GQ F ILEFGAI+VCPKKL ELES++TL++P DLS++S  S R 
Sbjct: 8   NEIVFFDLETNVPNKAGQHFHILEFGAIIVCPKKLEELESFTTLIQPKDLSVVSIRSSRS 67

Query: 68  NGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVPAPEPKGT 127
           +GITR  V ++P+F  +A++++ +L+GRIWAGHNI RFDC RI+EAFAEIG  APEP G 
Sbjct: 68  DGITRAKVTNAPSFEDVAEKIHGLLNGRIWAGHNIRRFDCVRIKEAFAEIGKAAPEPSGI 127

Query: 128 IDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATVLFLESSL 187
           IDSL LL+ +FG+RAG+MKMA+LA+YFG+G Q HRSLDDVRMNLEVLK+CATVLFLES+L
Sbjct: 128 IDSLGLLSDKFGKRAGNMKMASLAAYFGLGVQKHRSLDDVRMNLEVLKHCATVLFLESTL 187

Query: 188 PEVFPENSWVSPNAVTRRR 207
           P         S   +TR R
Sbjct: 188 PNHLEGKWHTSSKIMTRSR 206

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BLAST of Cucsat.G14528 vs. NCBI nr
Match: XP_011660176.1 (protein NEN1 [Cucumis sativus] >KGN63687.1 hypothetical protein Csa_013241 [Cucumis sativus])

HSP 1 Score: 986 bits (2549), Expect = 0.0
Identity = 505/505 (100.00%), Postives = 505/505 (100.00%), Query Frame = 0


Query: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI 60
           MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI
Sbjct: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI 60

Query: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120
           SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP
Sbjct: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120

Query: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180
           APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV
Sbjct: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGNSSNNNSHASSSNIGGNPISLPVQQG 240
           LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGNSSNNNSHASSSNIGGNPISLPVQQG
Sbjct: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGNSSNNNSHASSSNIGGNPISLPVQQG 240

Query: 241 ETHPILSLVTICSEDRASILAEPSATESDSFNMHTTSDQITGATLETDINMEEHATVSTE 300
           ETHPILSLVTICSEDRASILAEPSATESDSFNMHTTSDQITGATLETDINMEEHATVSTE
Sbjct: 241 ETHPILSLVTICSEDRASILAEPSATESDSFNMHTTSDQITGATLETDINMEEHATVSTE 300

Query: 301 ASPSEDTSTSLCTTKFLEPDQVSVSFITASFVPFFRGSQRIQLWHKDDCLQLLCNNLRVR 360
           ASPSEDTSTSLCTTKFLEPDQVSVSFITASFVPFFRGSQRIQLWHKDDCLQLLCNNLRVR
Sbjct: 301 ASPSEDTSTSLCTTKFLEPDQVSVSFITASFVPFFRGSQRIQLWHKDDCLQLLCNNLRVR 360

Query: 361 FGISTKFTDYAGRPRLSFVVDVPPNLCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKNGY 420
           FGISTKFTDYAGRPRLSFVVDVPPNLCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKNGY
Sbjct: 361 FGISTKFTDYAGRPRLSFVVDVPPNLCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKNGY 420

Query: 421 FNYPTMRLHIPTAVSGDVANYATEMHQKEASGAVQRLIFSKFDAAELDSLIKPGAILDAF 480
           FNYPTMRLHIPTAVSGDVANYATEMHQKEASGAVQRLIFSKFDAAELDSLIKPGAILDAF
Sbjct: 421 FNYPTMRLHIPTAVSGDVANYATEMHQKEASGAVQRLIFSKFDAAELDSLIKPGAILDAF 480

Query: 481 ISLDTYDYQQSAGIRLVAKKLIIRS 505
           ISLDTYDYQQSAGIRLVAKKLIIRS
Sbjct: 481 ISLDTYDYQQSAGIRLVAKKLIIRS 505

[Close]

BLAST of Cucsat.G14528 vs. NCBI nr
Match: XP_008453220.1 (PREDICTED: protein NEN1 [Cucumis melo])

HSP 1 Score: 942 bits (2436), Expect = 0.0
Identity = 489/506 (96.64%), Postives = 493/506 (97.43%), Query Frame = 0


Query: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI 60
           MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI
Sbjct: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI 60

Query: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120
           SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFA+IGVP
Sbjct: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFADIGVP 120

Query: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180
           APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV
Sbjct: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGNSSNNNSHASSSNIGGNPISLPVQQG 240
           LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGNSSNNNSHASSSNIGGNPISL  QQG
Sbjct: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGNSSNNNSHASSSNIGGNPISLTDQQG 240

Query: 241 ETHPILSLVTICSEDRASILAEPSATESDSFNMHTTSDQITGATLETDINME-EHATVST 300
           E HPILSLVT  SED A  LAEPSATESDSFNMHT SDQITGATLETDINME EHATVST
Sbjct: 241 EAHPILSLVTCSSEDGALNLAEPSATESDSFNMHTISDQITGATLETDINMEGEHATVST 300

Query: 301 EASPSEDTSTSLCTTKFLEPDQVSVSFITASFVPFFRGSQRIQLWHKDDCLQLLCNNLRV 360
           EASPSEDTSTSL TTKFLEPDQVSV FITASFVPFFRGSQRIQLWHKDDCLQLLCNNLRV
Sbjct: 301 EASPSEDTSTSLHTTKFLEPDQVSVLFITASFVPFFRGSQRIQLWHKDDCLQLLCNNLRV 360

Query: 361 RFGISTKFTDYAGRPRLSFVVDVPPNLCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKNG 420
           RFGISTKFTDYAGRPRLSFVVDVPP+LCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKNG
Sbjct: 361 RFGISTKFTDYAGRPRLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKNG 420

Query: 421 YFNYPTMRLHIPTAVSGDVANYATEMHQKEASGAVQRLIFSKFDAAELDSLIKPGAILDA 480
           YFNYPTMRLHIPTAVSGDVANYATEM+QK+ASGAVQRLIFSKFDAAELDSLIKPGAILDA
Sbjct: 421 YFNYPTMRLHIPTAVSGDVANYATEMYQKDASGAVQRLIFSKFDAAELDSLIKPGAILDA 480

Query: 481 FISLDTYDYQQSAGIRLVAKKLIIRS 505
           FISLDTYDYQQSAGIRLVAKKLII S
Sbjct: 481 FISLDTYDYQQSAGIRLVAKKLIIHS 506

[Close]

BLAST of Cucsat.G14528 vs. NCBI nr
Match: KAA0057957.1 (protein NEN1 [Cucumis melo var. makuwa])

HSP 1 Score: 905 bits (2340), Expect = 0.0
Identity = 475/506 (93.87%), Postives = 481/506 (95.06%), Query Frame = 0


Query: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI 60
           MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI
Sbjct: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI 60

Query: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120
           SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFA+IGVP
Sbjct: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFADIGVP 120

Query: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180
           APEPKGTIDSLALLTQRFGRRAGDMK+ T  S         +SLDDVRMNLEVLKYCATV
Sbjct: 121 APEPKGTIDSLALLTQRFGRRAGDMKVMTEFSLLH-ANSGDQSLDDVRMNLEVLKYCATV 180

Query: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGNSSNNNSHASSSNIGGNPISLPVQQG 240
           LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGNSSNNNSHASSSNIGGNPISL  QQG
Sbjct: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGNSSNNNSHASSSNIGGNPISLTDQQG 240

Query: 241 ETHPILSLVTICSEDRASILAEPSATESDSFNMHTTSDQITGATLETDINME-EHATVST 300
           E HPILSLVT  SED A  LAEPSATESDSFNMHT SDQITGATLETDINME EHATVST
Sbjct: 241 EAHPILSLVTCSSEDGALNLAEPSATESDSFNMHTISDQITGATLETDINMEGEHATVST 300

Query: 301 EASPSEDTSTSLCTTKFLEPDQVSVSFITASFVPFFRGSQRIQLWHKDDCLQLLCNNLRV 360
           EASPSEDTSTSL TTKFLEPDQVSV FITASFVPFFRGSQRIQLWHKDDCLQLLCNNLRV
Sbjct: 301 EASPSEDTSTSLHTTKFLEPDQVSVLFITASFVPFFRGSQRIQLWHKDDCLQLLCNNLRV 360

Query: 361 RFGISTKFTDYAGRPRLSFVVDVPPNLCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKNG 420
           RFGISTKFTDYAGRPRLSFVVDVPP+LCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKNG
Sbjct: 361 RFGISTKFTDYAGRPRLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKNG 420

Query: 421 YFNYPTMRLHIPTAVSGDVANYATEMHQKEASGAVQRLIFSKFDAAELDSLIKPGAILDA 480
           YFNYPTMRLHIPTAVSGDVANYATEM+QK+ASGAVQRLIFSKFDAAELDSLIKPGAILDA
Sbjct: 421 YFNYPTMRLHIPTAVSGDVANYATEMYQKDASGAVQRLIFSKFDAAELDSLIKPGAILDA 480

Query: 481 FISLDTYDYQQSAGIRLVAKKLIIRS 505
           FISLDTYDYQQSAGIRLVAKKLII S
Sbjct: 481 FISLDTYDYQQSAGIRLVAKKLIIHS 505

[Close]

BLAST of Cucsat.G14528 vs. NCBI nr
Match: XP_038880150.1 (protein NEN1-like [Benincasa hispida])

HSP 1 Score: 890 bits (2299), Expect = 0.0
Identity = 467/507 (92.11%), Postives = 482/507 (95.07%), Query Frame = 0


Query: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI 60
           MG TEDRSEIAFFDVETTVPTRQGQ+FSILEFGAILVCP+KLVELESYSTLV+PSDLSLI
Sbjct: 1   MGSTEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 60

Query: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120
           SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP
Sbjct: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120

Query: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180
           APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV
Sbjct: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGNSSNNNSHASSSNIGGNPISLPVQQG 240
           LFLESSLPEVF ENSWVSPNAVTRRR AKSSPQGNSSNN++H+SSS IGGNPISL + QG
Sbjct: 181 LFLESSLPEVFRENSWVSPNAVTRRRTAKSSPQGNSSNNDTHSSSSRIGGNPISL-LDQG 240

Query: 241 ETHPILSLVTICSEDRASILAEPSATESDSFNMHTTSDQITGATLETDINMEE-HATVST 300
           ETHPILSLVT  SED AS LAE  ATESDSFNM T SDQITG +LETDI+MEE HATVST
Sbjct: 241 ETHPILSLVTCSSEDGASNLAESRATESDSFNMRTLSDQITGESLETDISMEEEHATVST 300

Query: 301 EASPSEDTSTSL-CTTKFLEPDQVSVSFITASFVPFFRGSQRIQLWHKDDCLQLLCNNLR 360
           EASPSEDTSTSL  TT+FLEPDQVSVSFITASFVPFFRGSQRIQL HKDDCLQLLCNNLR
Sbjct: 301 EASPSEDTSTSLSATTEFLEPDQVSVSFITASFVPFFRGSQRIQLSHKDDCLQLLCNNLR 360

Query: 361 VRFGISTKFTDYAGRPRLSFVVDVPPNLCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKN 420
           VRFGISTKFTDYAGRP+LSFVVDVPP+LCTVLEASDGVAQRLFSDSGSGSEWRP V RKN
Sbjct: 361 VRFGISTKFTDYAGRPKLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPVVMRKN 420

Query: 421 GYFNYPTMRLHIPTAVSGDVANYATEMHQKEASGAVQRLIFSKFDAAELDSLIKPGAILD 480
            YFNYPTMRLHIPTAVSGDVA YATEM+QKE+SGA QRLIFSKFDAAELDSLIKPGAILD
Sbjct: 421 DYFNYPTMRLHIPTAVSGDVAIYATEMYQKESSGAAQRLIFSKFDAAELDSLIKPGAILD 480

Query: 481 AFISLDTYDYQQSAGIRLVAKKLIIRS 505
           AFISLDTYDYQQSAGIRLVAK+LII S
Sbjct: 481 AFISLDTYDYQQSAGIRLVAKRLIIHS 506

[Close]

BLAST of Cucsat.G14528 vs. NCBI nr
Match: XP_022921575.1 (protein NEN1-like [Cucurbita moschata] >KAG6589518.1 Protein NEN2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 846 bits (2186), Expect = 1.41e-306
Identity = 445/522 (85.25%), Postives = 474/522 (90.80%), Query Frame = 0


Query: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI 60
           MGPT DRSEIAFFDVETTVPTRQGQ+FSILEFGAILVCP+KL ELESYSTLV+PSDLSLI
Sbjct: 1   MGPTGDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLDELESYSTLVRPSDLSLI 60

Query: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120
           SSLSVRCNGITRDAVIS+PTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIG+P
Sbjct: 61  SSLSVRCNGITRDAVISAPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP 120

Query: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180
           AP+PKGTIDSLALLTQ+FGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV
Sbjct: 121 APQPKGTIDSLALLTQKFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGNSSNN--------------NSHASSS 240
           LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQG ++NN              N+ +SSS
Sbjct: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGTNANNSAAKASPQGINANSNTRSSSS 240

Query: 241 NIGGNPISLPVQQGETHPILSLVTICSEDRAS-ILAEPSATESDSFNMHTTSDQITGATL 300
            IGG+PISL  QQGE HPILSLVT  SED AS ++A+  ATESDSFNM T SDQI G +L
Sbjct: 241 RIGGSPISLTDQQGEAHPILSLVTCSSEDGASNLVADSDATESDSFNMGTLSDQIAGESL 300

Query: 301 ETDINMEE-HATVSTEASPSEDTSTSLCT-TKFLEPDQVSVSFITASFVPFFRGSQRIQL 360
           ETD +MEE HA+VSTEASP+E+TSTS+ + T+FLEPDQVSVS ITASFVPFFRGSQRIQL
Sbjct: 301 ETDTSMEEEHASVSTEASPTENTSTSISSATEFLEPDQVSVSSITASFVPFFRGSQRIQL 360

Query: 361 WHKDDCLQLLCNNLRVRFGISTKFTDYAGRPRLSFVVDVPPNLCTVLEASDGVAQRLFSD 420
            HKDDCLQLLCNNLRVRFGISTKFTDYAGRPRL+FVVDVPP+LC VLEASDGVAQRLFS+
Sbjct: 361 SHKDDCLQLLCNNLRVRFGISTKFTDYAGRPRLNFVVDVPPSLCRVLEASDGVAQRLFSE 420

Query: 421 SGSGSEWRPAVTRKNGYFNYPTMRLHIPTAVSGDVANYATEMHQKEASGAVQRLIFSKFD 480
           SGSGSEWRP V RKNGYFNYPTMRLHIPTAVSGD+A YATEM+QKEASG  QRLIFSKFD
Sbjct: 421 SGSGSEWRPVVMRKNGYFNYPTMRLHIPTAVSGDMAIYATEMYQKEASGGAQRLIFSKFD 480

Query: 481 AAELDSLIKPGAILDAFISLDTYDYQQSAGIRLVAKKLIIRS 505
           A ELDSLI PG I+DAFISLDTYDYQQSAGIRLVAKKLII S
Sbjct: 481 AVELDSLINPGGIIDAFISLDTYDYQQSAGIRLVAKKLIIHS 522

[Close]

BLAST of Cucsat.G14528 vs. ExPASy TrEMBL
Match: A0A0A0LSC3 (Exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G009910 PE=4 SV=1)

HSP 1 Score: 986 bits (2549), Expect = 0.0
Identity = 505/505 (100.00%), Postives = 505/505 (100.00%), Query Frame = 0


Query: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI 60
           MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI
Sbjct: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI 60

Query: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120
           SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP
Sbjct: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120

Query: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180
           APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV
Sbjct: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGNSSNNNSHASSSNIGGNPISLPVQQG 240
           LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGNSSNNNSHASSSNIGGNPISLPVQQG
Sbjct: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGNSSNNNSHASSSNIGGNPISLPVQQG 240

Query: 241 ETHPILSLVTICSEDRASILAEPSATESDSFNMHTTSDQITGATLETDINMEEHATVSTE 300
           ETHPILSLVTICSEDRASILAEPSATESDSFNMHTTSDQITGATLETDINMEEHATVSTE
Sbjct: 241 ETHPILSLVTICSEDRASILAEPSATESDSFNMHTTSDQITGATLETDINMEEHATVSTE 300

Query: 301 ASPSEDTSTSLCTTKFLEPDQVSVSFITASFVPFFRGSQRIQLWHKDDCLQLLCNNLRVR 360
           ASPSEDTSTSLCTTKFLEPDQVSVSFITASFVPFFRGSQRIQLWHKDDCLQLLCNNLRVR
Sbjct: 301 ASPSEDTSTSLCTTKFLEPDQVSVSFITASFVPFFRGSQRIQLWHKDDCLQLLCNNLRVR 360

Query: 361 FGISTKFTDYAGRPRLSFVVDVPPNLCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKNGY 420
           FGISTKFTDYAGRPRLSFVVDVPPNLCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKNGY
Sbjct: 361 FGISTKFTDYAGRPRLSFVVDVPPNLCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKNGY 420

Query: 421 FNYPTMRLHIPTAVSGDVANYATEMHQKEASGAVQRLIFSKFDAAELDSLIKPGAILDAF 480
           FNYPTMRLHIPTAVSGDVANYATEMHQKEASGAVQRLIFSKFDAAELDSLIKPGAILDAF
Sbjct: 421 FNYPTMRLHIPTAVSGDVANYATEMHQKEASGAVQRLIFSKFDAAELDSLIKPGAILDAF 480

Query: 481 ISLDTYDYQQSAGIRLVAKKLIIRS 505
           ISLDTYDYQQSAGIRLVAKKLIIRS
Sbjct: 481 ISLDTYDYQQSAGIRLVAKKLIIRS 505

[Close]

BLAST of Cucsat.G14528 vs. ExPASy TrEMBL
Match: A0A1S3BWV4 (protein NEN1 OS=Cucumis melo OX=3656 GN=LOC103494007 PE=4 SV=1)

HSP 1 Score: 942 bits (2436), Expect = 0.0
Identity = 489/506 (96.64%), Postives = 493/506 (97.43%), Query Frame = 0


Query: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI 60
           MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI
Sbjct: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI 60

Query: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120
           SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFA+IGVP
Sbjct: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFADIGVP 120

Query: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180
           APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV
Sbjct: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGNSSNNNSHASSSNIGGNPISLPVQQG 240
           LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGNSSNNNSHASSSNIGGNPISL  QQG
Sbjct: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGNSSNNNSHASSSNIGGNPISLTDQQG 240

Query: 241 ETHPILSLVTICSEDRASILAEPSATESDSFNMHTTSDQITGATLETDINME-EHATVST 300
           E HPILSLVT  SED A  LAEPSATESDSFNMHT SDQITGATLETDINME EHATVST
Sbjct: 241 EAHPILSLVTCSSEDGALNLAEPSATESDSFNMHTISDQITGATLETDINMEGEHATVST 300

Query: 301 EASPSEDTSTSLCTTKFLEPDQVSVSFITASFVPFFRGSQRIQLWHKDDCLQLLCNNLRV 360
           EASPSEDTSTSL TTKFLEPDQVSV FITASFVPFFRGSQRIQLWHKDDCLQLLCNNLRV
Sbjct: 301 EASPSEDTSTSLHTTKFLEPDQVSVLFITASFVPFFRGSQRIQLWHKDDCLQLLCNNLRV 360

Query: 361 RFGISTKFTDYAGRPRLSFVVDVPPNLCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKNG 420
           RFGISTKFTDYAGRPRLSFVVDVPP+LCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKNG
Sbjct: 361 RFGISTKFTDYAGRPRLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKNG 420

Query: 421 YFNYPTMRLHIPTAVSGDVANYATEMHQKEASGAVQRLIFSKFDAAELDSLIKPGAILDA 480
           YFNYPTMRLHIPTAVSGDVANYATEM+QK+ASGAVQRLIFSKFDAAELDSLIKPGAILDA
Sbjct: 421 YFNYPTMRLHIPTAVSGDVANYATEMYQKDASGAVQRLIFSKFDAAELDSLIKPGAILDA 480

Query: 481 FISLDTYDYQQSAGIRLVAKKLIIRS 505
           FISLDTYDYQQSAGIRLVAKKLII S
Sbjct: 481 FISLDTYDYQQSAGIRLVAKKLIIHS 506

[Close]

BLAST of Cucsat.G14528 vs. ExPASy TrEMBL
Match: A0A5A7UTA8 (Protein NEN1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002600 PE=4 SV=1)

HSP 1 Score: 905 bits (2340), Expect = 0.0
Identity = 475/506 (93.87%), Postives = 481/506 (95.06%), Query Frame = 0


Query: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI 60
           MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI
Sbjct: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI 60

Query: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120
           SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFA+IGVP
Sbjct: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFADIGVP 120

Query: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180
           APEPKGTIDSLALLTQRFGRRAGDMK+ T  S         +SLDDVRMNLEVLKYCATV
Sbjct: 121 APEPKGTIDSLALLTQRFGRRAGDMKVMTEFSLLH-ANSGDQSLDDVRMNLEVLKYCATV 180

Query: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGNSSNNNSHASSSNIGGNPISLPVQQG 240
           LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGNSSNNNSHASSSNIGGNPISL  QQG
Sbjct: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGNSSNNNSHASSSNIGGNPISLTDQQG 240

Query: 241 ETHPILSLVTICSEDRASILAEPSATESDSFNMHTTSDQITGATLETDINME-EHATVST 300
           E HPILSLVT  SED A  LAEPSATESDSFNMHT SDQITGATLETDINME EHATVST
Sbjct: 241 EAHPILSLVTCSSEDGALNLAEPSATESDSFNMHTISDQITGATLETDINMEGEHATVST 300

Query: 301 EASPSEDTSTSLCTTKFLEPDQVSVSFITASFVPFFRGSQRIQLWHKDDCLQLLCNNLRV 360
           EASPSEDTSTSL TTKFLEPDQVSV FITASFVPFFRGSQRIQLWHKDDCLQLLCNNLRV
Sbjct: 301 EASPSEDTSTSLHTTKFLEPDQVSVLFITASFVPFFRGSQRIQLWHKDDCLQLLCNNLRV 360

Query: 361 RFGISTKFTDYAGRPRLSFVVDVPPNLCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKNG 420
           RFGISTKFTDYAGRPRLSFVVDVPP+LCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKNG
Sbjct: 361 RFGISTKFTDYAGRPRLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKNG 420

Query: 421 YFNYPTMRLHIPTAVSGDVANYATEMHQKEASGAVQRLIFSKFDAAELDSLIKPGAILDA 480
           YFNYPTMRLHIPTAVSGDVANYATEM+QK+ASGAVQRLIFSKFDAAELDSLIKPGAILDA
Sbjct: 421 YFNYPTMRLHIPTAVSGDVANYATEMYQKDASGAVQRLIFSKFDAAELDSLIKPGAILDA 480

Query: 481 FISLDTYDYQQSAGIRLVAKKLIIRS 505
           FISLDTYDYQQSAGIRLVAKKLII S
Sbjct: 481 FISLDTYDYQQSAGIRLVAKKLIIHS 505

[Close]

BLAST of Cucsat.G14528 vs. ExPASy TrEMBL
Match: A0A6J1E665 (protein NEN1-like OS=Cucurbita moschata OX=3662 GN=LOC111429799 PE=4 SV=1)

HSP 1 Score: 846 bits (2186), Expect = 6.80e-307
Identity = 445/522 (85.25%), Postives = 474/522 (90.80%), Query Frame = 0


Query: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI 60
           MGPT DRSEIAFFDVETTVPTRQGQ+FSILEFGAILVCP+KL ELESYSTLV+PSDLSLI
Sbjct: 1   MGPTGDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLDELESYSTLVRPSDLSLI 60

Query: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120
           SSLSVRCNGITRDAVIS+PTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIG+P
Sbjct: 61  SSLSVRCNGITRDAVISAPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP 120

Query: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180
           AP+PKGTIDSLALLTQ+FGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV
Sbjct: 121 APQPKGTIDSLALLTQKFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGNSSNN--------------NSHASSS 240
           LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQG ++NN              N+ +SSS
Sbjct: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGTNANNSAAKASPQGINANSNTRSSSS 240

Query: 241 NIGGNPISLPVQQGETHPILSLVTICSEDRAS-ILAEPSATESDSFNMHTTSDQITGATL 300
            IGG+PISL  QQGE HPILSLVT  SED AS ++A+  ATESDSFNM T SDQI G +L
Sbjct: 241 RIGGSPISLTDQQGEAHPILSLVTCSSEDGASNLVADSDATESDSFNMGTLSDQIAGESL 300

Query: 301 ETDINMEE-HATVSTEASPSEDTSTSLCT-TKFLEPDQVSVSFITASFVPFFRGSQRIQL 360
           ETD +MEE HA+VSTEASP+E+TSTS+ + T+FLEPDQVSVS ITASFVPFFRGSQRIQL
Sbjct: 301 ETDTSMEEEHASVSTEASPTENTSTSISSATEFLEPDQVSVSSITASFVPFFRGSQRIQL 360

Query: 361 WHKDDCLQLLCNNLRVRFGISTKFTDYAGRPRLSFVVDVPPNLCTVLEASDGVAQRLFSD 420
            HKDDCLQLLCNNLRVRFGISTKFTDYAGRPRL+FVVDVPP+LC VLEASDGVAQRLFS+
Sbjct: 361 SHKDDCLQLLCNNLRVRFGISTKFTDYAGRPRLNFVVDVPPSLCRVLEASDGVAQRLFSE 420

Query: 421 SGSGSEWRPAVTRKNGYFNYPTMRLHIPTAVSGDVANYATEMHQKEASGAVQRLIFSKFD 480
           SGSGSEWRP V RKNGYFNYPTMRLHIPTAVSGD+A YATEM+QKEASG  QRLIFSKFD
Sbjct: 421 SGSGSEWRPVVMRKNGYFNYPTMRLHIPTAVSGDMAIYATEMYQKEASGGAQRLIFSKFD 480

Query: 481 AAELDSLIKPGAILDAFISLDTYDYQQSAGIRLVAKKLIIRS 505
           A ELDSLI PG I+DAFISLDTYDYQQSAGIRLVAKKLII S
Sbjct: 481 AVELDSLINPGGIIDAFISLDTYDYQQSAGIRLVAKKLIIHS 522

[Close]

BLAST of Cucsat.G14528 vs. ExPASy TrEMBL
Match: A0A6J1JJE4 (protein NEN1-like OS=Cucurbita maxima OX=3661 GN=LOC111484975 PE=4 SV=1)

HSP 1 Score: 843 bits (2177), Expect = 1.60e-305
Identity = 445/522 (85.25%), Postives = 470/522 (90.04%), Query Frame = 0


Query: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI 60
           MGPT DRSEIAFFDVETTVPTRQGQ+FSILEFGAILVCP+KL ELESYSTLV+PSDLSLI
Sbjct: 1   MGPTGDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLDELESYSTLVRPSDLSLI 60

Query: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120
           SSLSVRCNGITRDAVIS+PTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIG+P
Sbjct: 61  SSLSVRCNGITRDAVISAPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP 120

Query: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180
           AP+PKGTIDSLALLTQ+FGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV
Sbjct: 121 APQPKGTIDSLALLTQKFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGNSSNN--------------NSHASSS 240
           LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQG + NN              N+ +SSS
Sbjct: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGINENNSAAKSSPRGINANSNTRSSSS 240

Query: 241 NIGGNPISLPVQQGETHPILSLVTICSEDRAS-ILAEPSATESDSFNMHTTSDQITGATL 300
            IGG+PISL  QQGE HPILSLVT  SED AS ++A+  ATESDSFNM T SDQI G +L
Sbjct: 241 RIGGSPISLTDQQGEAHPILSLVTCSSEDGASNLVADSDATESDSFNMGTLSDQIAGESL 300

Query: 301 ETDINMEE-HATVSTEASPSEDTSTSL-CTTKFLEPDQVSVSFITASFVPFFRGSQRIQL 360
           ETD +MEE HA VSTEASP+E+TSTSL   T+FLEPDQVSVS ITASFVPFFRGSQRIQL
Sbjct: 301 ETDTSMEEEHAPVSTEASPTENTSTSLSAATEFLEPDQVSVSSITASFVPFFRGSQRIQL 360

Query: 361 WHKDDCLQLLCNNLRVRFGISTKFTDYAGRPRLSFVVDVPPNLCTVLEASDGVAQRLFSD 420
            HKDDCLQLLCNNLRVRFGISTKFTDYAGRPRL+FVVDVPP+LC VLEASDGVAQRLFS+
Sbjct: 361 SHKDDCLQLLCNNLRVRFGISTKFTDYAGRPRLNFVVDVPPSLCRVLEASDGVAQRLFSE 420

Query: 421 SGSGSEWRPAVTRKNGYFNYPTMRLHIPTAVSGDVANYATEMHQKEASGAVQRLIFSKFD 480
           SGSGSEWRP V RKNGYFNYPTMRLHIPTAV GD+A YATEM+QKEASG  QRLIFSKFD
Sbjct: 421 SGSGSEWRPVVMRKNGYFNYPTMRLHIPTAVCGDMAIYATEMYQKEASGGAQRLIFSKFD 480

Query: 481 AAELDSLIKPGAILDAFISLDTYDYQQSAGIRLVAKKLIIRS 505
           A ELDSLI PG I+DAFISLDTYDYQQSAGIRLVAKKLII S
Sbjct: 481 AVELDSLINPGGIIDAFISLDTYDYQQSAGIRLVAKKLIIHS 522

The following BLAST results are available for this feature:

BLAST of Cucsat.G14528 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FLR0	5.3e-151	57.74	Protein NEN1 OS=Arabidopsis thaliana OX=3702 GN=NEN1 PE=2 SV=1	[more]
Q0V842	4.3e-145	56.10	Protein NEN2 OS=Arabidopsis thaliana OX=3702 GN=NEN2 PE=2 SV=1	[more]
Q9CA74	5.8e-142	54.47	Protein NEN3 OS=Arabidopsis thaliana OX=3702 GN=NEN3 PE=2 SV=1	[more]
F4JJ23	5.0e-69	63.32	Protein NEN4 OS=Arabidopsis thaliana OX=3702 GN=NEN4 PE=2 SV=1	[more]

BLAST of Cucsat.G14528 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011660176.1	0.0	100.00	protein NEN1 [Cucumis sativus] >KGN63687.1 hypothetical protein Csa_013241 [Cucu...	[more]
XP_008453220.1	0.0	96.64	PREDICTED: protein NEN1 [Cucumis melo]	[more]
KAA0057957.1	0.0	93.87	protein NEN1 [Cucumis melo var. makuwa]	[more]
XP_038880150.1	0.0	92.11	protein NEN1-like [Benincasa hispida]	[more]
XP_022921575.1	1.41e-306	85.25	protein NEN1-like [Cucurbita moschata] >KAG6589518.1 Protein NEN2, partial [Cucu...	[more]

BLAST of Cucsat.G14528 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LSC3	0.0	100.00	Exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G009910...	[more]
A0A1S3BWV4	0.0	96.64	protein NEN1 OS=Cucumis melo OX=3656 GN=LOC103494007 PE=4 SV=1	[more]
A0A5A7UTA8	0.0	93.87	Protein NEN1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002600 ...	[more]
A0A6J1E665	6.80e-307	85.25	protein NEN1-like OS=Cucurbita moschata OX=3662 GN=LOC111429799 PE=4 SV=1	[more]
A0A6J1JJE4	1.60e-305	85.25	protein NEN1-like OS=Cucurbita maxima OX=3661 GN=LOC111484975 PE=4 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 34..69
None	No IPR available	PANTHER	PTHR30231	DNA POLYMERASE III SUBUNIT EPSILON	coord: 1..336
None	No IPR available	PANTHER	PTHR30231:SF27	PROTEIN NEN3	coord: 1..336

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G14528.T1	Cucsat.G14528.T1	mRNA
Cucsat.G14528.T4	Cucsat.G14528.T4	mRNA
Cucsat.G14528.T3	Cucsat.G14528.T3	mRNA
Cucsat.G14528.T5	Cucsat.G14528.T5	mRNA
Cucsat.G14528.T2	Cucsat.G14528.T2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
molecular_function	GO:0008408	3'-5' exonuclease activity
molecular_function	GO:0004386	helicase activity
molecular_function	GO:0003676	nucleic acid binding