Cucsat.G13649 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G13649
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	RING-type E3 ubiquitin transferase
Location	ctg184: 261532 .. 262289 (-)
RNA-Seq Expression	Cucsat.G13649
Synteny	Cucsat.G13649

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CTGGGTATGTTGATTTCTTTGCTTACTGATGCTTTATGTTACTCTACTGTTGATTATTCAATTGGGTGTTCAAATTTTCTCTTGGCTTTTGTTTGATTTCGTAGTTCTTGTATATTTTCTTTCTATACATTGTTTATCACTGTGCTGCATTCTGGTTATGTTTATGCCTGTTCGATGATTTTGAAACGATGGTGAATTTGTCTGTTGTTTTTCTTTTTGATTCTTAACTGAAGTGGTTTGGATGAGAAAAATGCTGGCTTCTAGTTTTAAACAGAGATGATTTAATACTCGTTCTATTAATAGATTATAGGTTGTTTTCTTTTGGTTTTCTTGTATTTTACTTTTCTGTTTATAGATTCTTATCGCAGCACAGATGCCAAACTCAGTAAAGAGGAAAAGAGAAAGGAAAAAAAATTGGTGCTTCTGTTGGGTTTTGTTTTCTTGCTGCTTGTACTTGTGCCAATTAATGATATAGCTTTTATTTTCTCACTCTTAAACGCACTTTTTCTGTTGTGCAAGCAAACAATGATTCCATCCGATTGTAACTGTCATGTGTTGTTGTAAATTTTAATGCATACATGAGTTTTCAATTTAAAGAACGTTGAAGATTCTTCCTCGTAGCTTCCGTTTCCAACAGGTTCTGTTCTGTCTATGGTATGTTATTATAAAAGAAGGATTTAATGATCCAAAACTTAATTGATTTTACTTCTTGCGCATGCAATGCTTTCAAATTGCATCTTGCTTCTCATTGCTCTTGATTACTGGATGTGAAATTCATTTTGGTATGTAATAAATGCACACAGTTATTGTCCTTGATTTGTGATTTAGGAGATCCATGTTTTGAATGTTTTTTCTATTATTATCCTATTTGCATTGCACATTTGAAAATAAATATCATTGAGTGTGAAGTTTAAATGGAATCCACATAGCTGAAATGATCATCCTACTAAATTGATTGGTCCAGGGAACAAGTTTTGAGATGCTTGCTTATCATAAGTTTCTACGGAACCAATTATGAGTTCTTCAAACAAGTCCCTCTAGCAATGACACTTCTTCTTTTTTAATTAAATCTCAGAGATGATTGTATGAGTACCATAATAGAATTCAGAGAGAAGGGGATGTATCAGCAACCCAAGTCAAGGGAAGAACATGCTTCTTCTGCAGCCAGCTTCTGCAAAGAAGTGACCGAGTTGCGCCTTACATCGATAGATTCTCAACCAGCAACTTCTCGTCCTGAGTTCAAAGGTGTCACTTTCAGGAAAGCTTCAACTGGTGGCTTTGCTAGGAGTCGTAATGATGTACACGAGCTACTTGATTGTACTGTTTGCATGAATTTAATGCATCCTCCAATTTACCAGGTATCGTATTCTTATTGTGATGACTTTCAAAACATATAACGCTTTGCTCATTGATTATTCTGAAAAAAAAAACGTTTCATAGATAAATAAATTCATCCAATAAATAGAACACTGACGCATTTAATTTGGTTGAAGGCCTAGAACCCTAATGGATTATTGAGTCACTCTTTTGTCCCCGGAAATAGCTTTCGGACCAAGTATCAAAAGGACTCTATATAACTTCACTTTCTGTTCAAATGGAAGGCTAGGAATTTTGTTTAAGCGTTGATTTGCATTATGATTGAGTTATTACATTTGAGGTAACTCCAATGCATAACAGCTAACGTAATTTTACGGTTCAGTGTGCAAATGGCCACACTCTTTGTTCAAGCTGCAAGGCAAGGGTTCAGAATTGCTGCCCTACCTGTCGGCATGAGCTTGGGAACATAAGGTGCCTAGCACTGGAAAAGGTGGCCGAGTCCCTTGAGCTCCCTTGTGTGTACCAAAACCTAGGTTGCATTGACATGTTCCCATACTACAGCAAGCTTAAACACGAGAAGAATTGTAAATACCGGCCGTACAACTGCCCTTACGCTGGAGGTGAATGTCATGTAACTGGTGACATTCCATCCCTTGTCATGCATCTCAAGGATGATCACGAGGTGGATATGCATGATGGATGTTCCTTCAGTCATAGATATGTCAAATCCAATCCCCAAGAAGTTGAAAATGCCACATGGATGCTAACTGTAAGATGTCTCTCTTTATTTCAGATTCTTAGGAATAATGAAAGCAAAGAAGAAGCAATTTCATGTAATGAACATCCTCCTGATTTCTCAATTGATCCAATTCAGGTTTTCAACTGCTTTGGACGACAATTCTGTTTGCACTTTGAAGCATTTCGCCTAGGAACAGCACCTGTTTACATGGCATTCCTCCGGTTCATGGGTGACGACAGCGAGGCAAAACAGTTCTCATACAGTCTTGAAGTTGGTGGAAATGGAAGAAAACTCATCTGGCAAGGTATTCCAAGAAGTATTCGCGACAGCCATCGGAAGGTTCGAGACAGTCATGATGGACTAATTATTCAGAGGAAATTGGCATTATTTTGCTCTGGAAACGAGGGGATGGAACTGAAGTTAAAGGTTACAGGACGAATATGGAGAGTGAGGTGACAACAACAAATGAATATTCATCTATCAAACTCAAAACTTGTACAATATGTTGTGACATTGTGACCTTTGTACATGCCTCGGCCCTCCTACAATTCAGCCTTTTTTTAGAACCTCTTATTTCTTCATCACCTCTACATTTTCTGCTTCATTTTCTTTTCTCTATAGTTTATTTATTTTTATCTAATTATTAAAATAGAATGATAGAAATAAAACTAACAAGAATGCCAACCCATTTTCATAGTAATGCAATGCTAAATGTAAAACAACTCAGCATATTATGCATAGAACTTAGAAGAATACAAAAAGGTGTTGGACAACTTTAACAGTTAAGGCTATTGAAGTTTTTACCTTTTTGGTTCTTCACCTTCTCTTCTTTTTATGTTGTTGAAAGATAACATTTGTTAGATAGTTTTAACAATTTATGTATCATAAATTTTGAAAGTTGATTAAACTTTGTAAATAATCTTAAATTAAATTGTGATA

Coding sequence (CDS)

ATGAGTACCATAATAGAATTCAGAGAGAAGGGGATGTATCAGCAACCCAAGTCAAGGGAAGAACATGCTTCTTCTGCAGCCAGCTTCTGCAAAGAAGTGACCGAGTTGCGCCTTACATCGATAGATTCTCAACCAGCAACTTCTCGTCCTGAGTTCAAAGGTGTCACTTTCAGGAAAGCTTCAACTGGTGGCTTTGCTAGGAGTCGTAATGATGTACACGAGCTACTTGATTGTACTGTTTGCATGAATTTAATGCATCCTCCAATTTACCAGTGTGCAAATGGCCACACTCTTTGTTCAAGCTGCAAGGCAAGGGTTCAGAATTGCTGCCCTACCTGTCGGCATGAGCTTGGGAACATAAGGTGCCTAGCACTGGAAAAGGTGGCCGAGTCCCTTGAGCTCCCTTGTGTGTACCAAAACCTAGGTTGCATTGACATGTTCCCATACTACAGCAAGCTTAAACACGAGAAGAATTGTAAATACCGGCCGTACAACTGCCCTTACGCTGGAGGTGAATGTCATGTAACTGGTGACATTCCATCCCTTGTCATGCATCTCAAGGATGATCACGAGGTGGATATGCATGATGGATGTTCCTTCAGTCATAGATATGTCAAATCCAATCCCCAAGAAGTTGAAAATGCCACATGGATGCTAACTGTTTTCAACTGCTTTGGACGACAATTCTGTTTGCACTTTGAAGCATTTCGCCTAGGAACAGCACCTGTTTACATGGCATTCCTCCGGTTCATGGGTGACGACAGCGAGGCAAAACAGTTCTCATACAGTCTTGAAGTTGGTGGAAATGGAAGAAAACTCATCTGGCAAGGTATTCCAAGAAGTATTCGCGACAGCCATCGGAAGGTTCGAGACAGTCATGATGGACTAATTATTCAGAGGAAATTGGCATTATTTTGCTCTGGAAACGAGGGGATGGAACTGAAGTTAAAGGTTACAGGACGAATATGGAGAGTGAGGTGA

Protein sequence

MSTIIEFREKGMYQQPKSREEHASSAASFCKEVTELRLTSIDSQPATSRPEFKGVTFRKASTGGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIPSLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDSEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNEGMELKLKVTGRIWRVR

Homology

[Close]

BLAST of Cucsat.G13649 vs. ExPASy Swiss-Prot
Match: Q10L91 (E3 ubiquitin-protein ligase DIS1 OS=Oryza sativa subsp. japonica OX=39947 GN=DIS1 PE=1 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 1.4e-48
Identity = 92/140 (65.71%), Postives = 110/140 (78.57%), Query Frame = 0


Query: 1   HLKDDHEILRNN--ESKEEAISCNEHPPDFSIDPIQVFNCFGRQFCLHFEAFRLGTAPVY 60
           HLKDDH++  +N        +  N H  + +   + VF+CFG+ FCLHFEAF+LG APVY
Sbjct: 160 HLKDDHKVDMHNGCTFNHRYVKSNPHEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVY 219

Query: 61  MAFLRFMGDDSEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLAL 120
           +AFLRFMGDD EAK +SYSLEVGG GRK+IWQG+PRSIRDSHRKVRDS+DGLIIQR +AL
Sbjct: 220 IAFLRFMGDDLEAKNYSYSLEVGGTGRKMIWQGVPRSIRDSHRKVRDSYDGLIIQRNMAL 279

Query: 121 FCSGNEGMELKLKVTGRIWR 139
           F SG E  ELKL+VTGRIW+
Sbjct: 280 FFSGGERKELKLRVTGRIWK 299

[Close]

BLAST of Cucsat.G13649 vs. ExPASy Swiss-Prot
Match: Q9M2P4 (E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana OX=3702 GN=SINAT2 PE=1 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 1.8e-48
Identity = 93/140 (66.43%), Postives = 110/140 (78.57%), Query Frame = 0


Query: 1   HLKDDHEILRNN--ESKEEAISCNEHPPDFSIDPIQVFNCFGRQFCLHFEAFRLGTAPVY 60
           HLKDDH++  ++        +  N H  + +   + VFNCFGRQFCLHFEAF+LG APVY
Sbjct: 167 HLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVY 226

Query: 61  MAFLRFMGDDSEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLAL 120
           MAFLRFMGD++EAK+FSYSLEVG +GRKL WQGIPRSIRDSHRKVRDS DGLII R LAL
Sbjct: 227 MAFLRFMGDENEAKKFSYSLEVGAHGRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLAL 286

Query: 121 FCSGNEGMELKLKVTGRIWR 139
           + SG +  ELKL+VTGRIW+
Sbjct: 287 YFSGGDRQELKLRVTGRIWK 306

[Close]

BLAST of Cucsat.G13649 vs. ExPASy Swiss-Prot
Match: P93748 (Putative E3 ubiquitin-protein ligase SINAT1 OS=Arabidopsis thaliana OX=3702 GN=SINAT1 PE=1 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 1.2e-47
Identity = 92/140 (65.71%), Postives = 110/140 (78.57%), Query Frame = 0


Query: 1   HLKDDHEILRNN--ESKEEAISCNEHPPDFSIDPIQVFNCFGRQFCLHFEAFRLGTAPVY 60
           HLKDDH++  ++        +  N H  + +   + VFNCFGRQFCLHFEAF+LG APVY
Sbjct: 164 HLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVY 223

Query: 61  MAFLRFMGDDSEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLAL 120
           MAFLRFMGD++EAK+FSYSLEVG + RKL WQGIPRSIRDSHRKVRDS DGLII R LAL
Sbjct: 224 MAFLRFMGDENEAKKFSYSLEVGAHSRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLAL 283

Query: 121 FCSGNEGMELKLKVTGRIWR 139
           + SG++  ELKL+VTGRIW+
Sbjct: 284 YFSGSDKEELKLRVTGRIWK 303

[Close]

BLAST of Cucsat.G13649 vs. ExPASy Swiss-Prot
Match: Q84JL3 (E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana OX=3702 GN=SINAT3 PE=1 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 8.3e-46
Identity = 86/140 (61.43%), Postives = 109/140 (77.86%), Query Frame = 0


Query: 1   HLKDDHEILRNN--ESKEEAISCNEHPPDFSIDPIQVFNCFGRQFCLHFEAFRLGTAPVY 60
           HL+DDH++  ++        +  N    + +   + VF+CFG+ FCLHFEAF+LG APVY
Sbjct: 170 HLRDDHKVDMHSGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVY 229

Query: 61  MAFLRFMGDDSEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLAL 120
           MAFLRFMGD++EA+ ++YSLEVGG GRKLIW+G PRS+RDSHRKVRDSHDGLIIQR +AL
Sbjct: 230 MAFLRFMGDETEARNYNYSLEVGGYGRKLIWEGTPRSVRDSHRKVRDSHDGLIIQRNMAL 289

Query: 121 FCSGNEGMELKLKVTGRIWR 139
           F SG +  ELKL+VTGRIW+
Sbjct: 290 FFSGGDRKELKLRVTGRIWK 309

[Close]

BLAST of Cucsat.G13649 vs. ExPASy Swiss-Prot
Match: Q9STN8 (E3 ubiquitin-protein ligase SINAT4 OS=Arabidopsis thaliana OX=3702 GN=SINAT4 PE=1 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 3.5e-44
Identity = 84/140 (60.00%), Postives = 107/140 (76.43%), Query Frame = 0


Query: 1   HLKDDHEILRNNES--KEEAISCNEHPPDFSIDPIQVFNCFGRQFCLHFEAFRLGTAPVY 60
           HL+DDH++  +  S      +  N    + +   + VF+CFG+ FCLHFEAF+LG  PVY
Sbjct: 171 HLRDDHKVDMHAGSTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMGPVY 230

Query: 61  MAFLRFMGDDSEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLAL 120
           MAFLRFMGD+ +A+ +SYSLEVGG+GRKL W+G PRSIRDSHRKVRDS+DGLIIQR +AL
Sbjct: 231 MAFLRFMGDEEDARSYSYSLEVGGSGRKLTWEGTPRSIRDSHRKVRDSNDGLIIQRNMAL 290

Query: 121 FCSGNEGMELKLKVTGRIWR 139
           F SG +  ELKL+VTG+IW+
Sbjct: 291 FFSGGDRKELKLRVTGKIWK 310

[Close]

BLAST of Cucsat.G13649 vs. NCBI nr
Match: XP_031740164.1 (E3 ubiquitin-protein ligase SINAT2-like [Cucumis sativus] >KGN54095.1 hypothetical protein Csa_018085 [Cucumis sativus])

HSP 1 Score: 229 bits (585), Expect = 1.91e-72
Identity = 114/142 (80.28%), Postives = 121/142 (85.21%), Query Frame = 0


Query: 1   HLKDDHEILRNN--ESKEEAISCNEHPPDFSIDPIQVFNCFGRQFCLHFEAFRLGTAPVY 60
           HLKDDHE+  ++        +  N    + +   + VFNCFGRQFCLHFEAFRLGTAPVY
Sbjct: 185 HLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVY 244

Query: 61  MAFLRFMGDDSEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLAL 120
           MAFLRFMGDDSEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLAL
Sbjct: 245 MAFLRFMGDDSEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLAL 304

Query: 121 FCSGNEGMELKLKVTGRIWRVR 140
           FCSGNEGMELKLKVTGRIWRVR
Sbjct: 305 FCSGNEGMELKLKVTGRIWRVR 326

[Close]

BLAST of Cucsat.G13649 vs. NCBI nr
Match: KAA0040047.1 (E3 ubiquitin-protein ligase SINAT2 [Cucumis melo var. makuwa] >TYK21670.1 E3 ubiquitin-protein ligase SINAT2 [Cucumis melo var. makuwa])

HSP 1 Score: 228 bits (582), Expect = 3.91e-72
Identity = 113/142 (79.58%), Postives = 121/142 (85.21%), Query Frame = 0


Query: 1   HLKDDHEILRNN--ESKEEAISCNEHPPDFSIDPIQVFNCFGRQFCLHFEAFRLGTAPVY 60
           HLKDDHE+  ++        +  N    + +   + VFNCFGRQFCLHFEAFRLGTAPVY
Sbjct: 174 HLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVY 233

Query: 61  MAFLRFMGDDSEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLAL 120
           MAFLRFMGDD+EAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLAL
Sbjct: 234 MAFLRFMGDDNEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLAL 293

Query: 121 FCSGNEGMELKLKVTGRIWRVR 140
           FCSGNEGMELKLKVTGRIWRVR
Sbjct: 294 FCSGNEGMELKLKVTGRIWRVR 315

[Close]

BLAST of Cucsat.G13649 vs. NCBI nr
Match: XP_008449780.1 (PREDICTED: E3 ubiquitin-protein ligase SINAT2 [Cucumis melo])

HSP 1 Score: 228 bits (582), Expect = 5.43e-72
Identity = 113/142 (79.58%), Postives = 121/142 (85.21%), Query Frame = 0


Query: 1   HLKDDHEILRNN--ESKEEAISCNEHPPDFSIDPIQVFNCFGRQFCLHFEAFRLGTAPVY 60
           HLKDDHE+  ++        +  N    + +   + VFNCFGRQFCLHFEAFRLGTAPVY
Sbjct: 185 HLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVY 244

Query: 61  MAFLRFMGDDSEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLAL 120
           MAFLRFMGDD+EAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLAL
Sbjct: 245 MAFLRFMGDDNEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLAL 304

Query: 121 FCSGNEGMELKLKVTGRIWRVR 140
           FCSGNEGMELKLKVTGRIWRVR
Sbjct: 305 FCSGNEGMELKLKVTGRIWRVR 326

[Close]

BLAST of Cucsat.G13649 vs. NCBI nr
Match: XP_038900385.1 (E3 ubiquitin-protein ligase SINAT2-like isoform X4 [Benincasa hispida])

HSP 1 Score: 225 bits (573), Expect = 1.36e-70
Identity = 111/141 (78.72%), Postives = 119/141 (84.40%), Query Frame = 0


Query: 1   HLKDDHEILRNN--ESKEEAISCNEHPPDFSIDPIQVFNCFGRQFCLHFEAFRLGTAPVY 60
           HLKDDHE+  ++        +  N    + +   + VFNCFGRQFCLHFEAFRLGTAPVY
Sbjct: 174 HLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVY 233

Query: 61  MAFLRFMGDDSEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLAL 120
           MAFLRFMGDD +AKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLAL
Sbjct: 234 MAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLAL 293

Query: 121 FCSGNEGMELKLKVTGRIWRV 139
           FCSGNEGMELKLKVTGRIWRV
Sbjct: 294 FCSGNEGMELKLKVTGRIWRV 314

[Close]

BLAST of Cucsat.G13649 vs. NCBI nr
Match: XP_038900384.1 (E3 ubiquitin-protein ligase SINAT2-like isoform X3 [Benincasa hispida] >XP_038900386.1 E3 ubiquitin-protein ligase SINAT2-like isoform X3 [Benincasa hispida])

HSP 1 Score: 225 bits (573), Expect = 1.83e-70
Identity = 111/141 (78.72%), Postives = 119/141 (84.40%), Query Frame = 0


Query: 1   HLKDDHEILRNN--ESKEEAISCNEHPPDFSIDPIQVFNCFGRQFCLHFEAFRLGTAPVY 60
           HLKDDHE+  ++        +  N    + +   + VFNCFGRQFCLHFEAFRLGTAPVY
Sbjct: 184 HLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVY 243

Query: 61  MAFLRFMGDDSEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLAL 120
           MAFLRFMGDD +AKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLAL
Sbjct: 244 MAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLAL 303

Query: 121 FCSGNEGMELKLKVTGRIWRV 139
           FCSGNEGMELKLKVTGRIWRV
Sbjct: 304 FCSGNEGMELKLKVTGRIWRV 324

[Close]

BLAST of Cucsat.G13649 vs. ExPASy TrEMBL
Match: A0A0A0KWV9 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G285710 PE=3 SV=1)

HSP 1 Score: 229 bits (585), Expect = 9.24e-73
Identity = 114/142 (80.28%), Postives = 121/142 (85.21%), Query Frame = 0


Query: 1   HLKDDHEILRNN--ESKEEAISCNEHPPDFSIDPIQVFNCFGRQFCLHFEAFRLGTAPVY 60
           HLKDDHE+  ++        +  N    + +   + VFNCFGRQFCLHFEAFRLGTAPVY
Sbjct: 185 HLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVY 244

Query: 61  MAFLRFMGDDSEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLAL 120
           MAFLRFMGDDSEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLAL
Sbjct: 245 MAFLRFMGDDSEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLAL 304

Query: 121 FCSGNEGMELKLKVTGRIWRVR 140
           FCSGNEGMELKLKVTGRIWRVR
Sbjct: 305 FCSGNEGMELKLKVTGRIWRVR 326

[Close]

BLAST of Cucsat.G13649 vs. ExPASy TrEMBL
Match: A0A5D3DDZ5 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G00330 PE=3 SV=1)

HSP 1 Score: 228 bits (582), Expect = 1.89e-72
Identity = 113/142 (79.58%), Postives = 121/142 (85.21%), Query Frame = 0


Query: 1   HLKDDHEILRNN--ESKEEAISCNEHPPDFSIDPIQVFNCFGRQFCLHFEAFRLGTAPVY 60
           HLKDDHE+  ++        +  N    + +   + VFNCFGRQFCLHFEAFRLGTAPVY
Sbjct: 174 HLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVY 233

Query: 61  MAFLRFMGDDSEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLAL 120
           MAFLRFMGDD+EAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLAL
Sbjct: 234 MAFLRFMGDDNEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLAL 293

Query: 121 FCSGNEGMELKLKVTGRIWRVR 140
           FCSGNEGMELKLKVTGRIWRVR
Sbjct: 294 FCSGNEGMELKLKVTGRIWRVR 315

[Close]

BLAST of Cucsat.G13649 vs. ExPASy TrEMBL
Match: A0A1S3BMT4 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103491565 PE=3 SV=1)

HSP 1 Score: 228 bits (582), Expect = 2.63e-72
Identity = 113/142 (79.58%), Postives = 121/142 (85.21%), Query Frame = 0


Query: 1   HLKDDHEILRNN--ESKEEAISCNEHPPDFSIDPIQVFNCFGRQFCLHFEAFRLGTAPVY 60
           HLKDDHE+  ++        +  N    + +   + VFNCFGRQFCLHFEAFRLGTAPVY
Sbjct: 185 HLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVY 244

Query: 61  MAFLRFMGDDSEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLAL 120
           MAFLRFMGDD+EAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLAL
Sbjct: 245 MAFLRFMGDDNEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLAL 304

Query: 121 FCSGNEGMELKLKVTGRIWRVR 140
           FCSGNEGMELKLKVTGRIWRVR
Sbjct: 305 FCSGNEGMELKLKVTGRIWRVR 326

[Close]

BLAST of Cucsat.G13649 vs. ExPASy TrEMBL
Match: A0A6J1DI06 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111021266 PE=3 SV=1)

HSP 1 Score: 209 bits (532), Expect = 9.23e-65
Identity = 103/142 (72.54%), Postives = 112/142 (78.87%), Query Frame = 0


Query: 1   HLKDDHEILRNN--ESKEEAISCNEHPPDFSIDPIQVFNCFGRQFCLHFEAFRLGTAPVY 60
           HLKDDHE+  +         +  N    + +   + VFNCFGRQFCLHFEAFRLGTAPVY
Sbjct: 184 HLKDDHEVDMHEGCSFSHRYVKSNPREVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVY 243

Query: 61  MAFLRFMGDDSEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLAL 120
           MAFLRFMGDD +AK+FSYSLEVGGNGRKL+WQG PRSIRDSHRKVRDS DGLII R LAL
Sbjct: 244 MAFLRFMGDDDDAKKFSYSLEVGGNGRKLMWQGTPRSIRDSHRKVRDSQDGLIIHRNLAL 303

Query: 121 FCSGNEGMELKLKVTGRIWRVR 140
           FCSGN G ELKLKV GRIWRVR
Sbjct: 304 FCSGNHGQELKLKVVGRIWRVR 325

[Close]

BLAST of Cucsat.G13649 vs. ExPASy TrEMBL
Match: A0A7J7BV06 (RING-type E3 ubiquitin transferase OS=Tripterygium wilfordii OX=458696 GN=HS088_TW23G00228 PE=3 SV=1)

HSP 1 Score: 200 bits (509), Expect = 1.74e-61
Identity = 97/140 (69.29%), Postives = 111/140 (79.29%), Query Frame = 0


Query: 1   HLKDDHEILRNNES--KEEAISCNEHPPDFSIDPIQVFNCFGRQFCLHFEAFRLGTAPVY 60
           HLK+DH++  +N S  K   + CN H  + +     VFNCFGRQFCLHFEAF +G APVY
Sbjct: 168 HLKEDHKVELHNGSTFKHRYVKCNPHEVENATWMSTVFNCFGRQFCLHFEAFHIGMAPVY 227

Query: 61  MAFLRFMGDDSEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLAL 120
           M FLRFMGD+ EA+QF+YSLEVGGNGRKL WQGIPRSIRDSH+KVRDS DGLIIQR LAL
Sbjct: 228 MTFLRFMGDEDEARQFTYSLEVGGNGRKLTWQGIPRSIRDSHKKVRDSQDGLIIQRNLAL 287

Query: 121 FCSGNEGMELKLKVTGRIWR 138
           F SGN+  ELKLKV GRIW+
Sbjct: 288 FFSGNDRQELKLKVAGRIWK 307

The following BLAST results are available for this feature:

BLAST of Cucsat.G13649 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q10L91	1.4e-48	65.71	E3 ubiquitin-protein ligase DIS1 OS=Oryza sativa subsp. japonica OX=39947 GN=DIS...	[more]
Q9M2P4	1.8e-48	66.43	E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana OX=3702 GN=SINAT2 PE=...	[more]
P93748	1.2e-47	65.71	Putative E3 ubiquitin-protein ligase SINAT1 OS=Arabidopsis thaliana OX=3702 GN=S...	[more]
Q84JL3	8.3e-46	61.43	E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana OX=3702 GN=SINAT3 PE=...	[more]
Q9STN8	3.5e-44	60.00	E3 ubiquitin-protein ligase SINAT4 OS=Arabidopsis thaliana OX=3702 GN=SINAT4 PE=...	[more]

BLAST of Cucsat.G13649 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031740164.1	1.91e-72	80.28	E3 ubiquitin-protein ligase SINAT2-like [Cucumis sativus] >KGN54095.1 hypothetic...	[more]
KAA0040047.1	3.91e-72	79.58	E3 ubiquitin-protein ligase SINAT2 [Cucumis melo var. makuwa] >TYK21670.1 E3 ubi...	[more]
XP_008449780.1	5.43e-72	79.58	PREDICTED: E3 ubiquitin-protein ligase SINAT2 [Cucumis melo]	[more]
XP_038900385.1	1.36e-70	78.72	E3 ubiquitin-protein ligase SINAT2-like isoform X4 [Benincasa hispida]	[more]
XP_038900384.1	1.83e-70	78.72	E3 ubiquitin-protein ligase SINAT2-like isoform X3 [Benincasa hispida] >XP_03890...	[more]

BLAST of Cucsat.G13649 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KWV9	9.24e-73	80.28	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G285710 PE...	[more]
A0A5D3DDZ5	1.89e-72	79.58	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3BMT4	2.63e-72	79.58	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103491565 PE=3 ...	[more]
A0A6J1DI06	9.23e-65	72.54	RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11102126...	[more]
A0A7J7BV06	1.74e-61	69.29	RING-type E3 ubiquitin transferase OS=Tripterygium wilfordii OX=458696 GN=HS088_...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 14..130 e-value: 2.6E-7 score: 32.2
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 131..186 e-value: 3.8E-16 score: 61.1
IPR018121	Seven-in-absentia protein, TRAF-like domain	PFAM	PF03145	Sina	coord: 120..319 e-value: 1.6E-79 score: 266.6
IPR008974	TRAF-like	GENE3D	2.60.210.10	Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2; Chain A	coord: 187..319 e-value: 3.5E-36 score: 125.9
None	No IPR available	PANTHER	PTHR10315:SF123	E3 UBIQUITIN-PROTEIN LIGASE SINAT2-LIKE	coord: 27..324
None	No IPR available	PANTHER	PTHR10315	E3 UBIQUITIN PROTEIN LIGASE SIAH	coord: 27..324
None	No IPR available	CDD	cd16571	RING-HC_SIAHs	coord: 76..114 e-value: 1.0421E-12 score: 59.6058
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 60..118
None	No IPR available	SUPERFAMILY	49599	TRAF domain-like	coord: 131..322
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 78..114 score: 9.214112
IPR013010	Zinc finger, SIAH-type	PROSITE	PS51081	ZF_SIAH	coord: 131..191 score: 13.758881

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G13649.T6	Cucsat.G13649.T6	mRNA
Cucsat.G13649.T1	Cucsat.G13649.T1	mRNA
Cucsat.G13649.T2	Cucsat.G13649.T2	mRNA
Cucsat.G13649.T4	Cucsat.G13649.T4	mRNA
Cucsat.G13649.T10	Cucsat.G13649.T10	mRNA
Cucsat.G13649.T9	Cucsat.G13649.T9	mRNA
Cucsat.G13649.T8	Cucsat.G13649.T8	mRNA
Cucsat.G13649.T3	Cucsat.G13649.T3	mRNA
Cucsat.G13649.T5	Cucsat.G13649.T5	mRNA
Cucsat.G13649.T11	Cucsat.G13649.T11	mRNA
Cucsat.G13649.T7	Cucsat.G13649.T7	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007275	multicellular organism development
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0005515	protein binding
molecular_function	GO:0016740	transferase activity
molecular_function	GO:0061630	ubiquitin protein ligase activity
molecular_function	GO:0008270	zinc ion binding