Cucsat.G12992 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G12992
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Rhamnogalacturonan endolyase
Location	ctg1838: 8613927 .. 8621021 (-)
RNA-Seq Expression	Cucsat.G12992
Synteny	Cucsat.G12992

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TATAAAAATCATTGATCGTACCGACCGCGCGCCAGTTCCGTGCTGTATTAAGTTCCAACCTTCGCCGTCGAGCAAAGTAATTCGCGAATCCTAATCGAAACAAAGAATGTGAAAATTCATTCTACAGTTTGATAATCGGAATCCGTTCATATTCTCAGTCGATCGTGTTGTTCATGCTATCAATTAGTTTCTCTTCTCTATCAATGTCCGCCATCATAACAAACTGTTCTTATTCTTTGTCCTTTGGATTTCCATTTTCCTTTTGATCTTCTACTTCTGTTTGCTCCACTGACTTTTCTTATAGAAACTTTGAATGTGAAAGTTTTACAATGTCATCCAGAGCCGTCCGCTTGCAGATACGAGATCATCATGTAAGTTAGATTTTCTATTTTGTTCCGACTCTGTAAAGATATTCCAGACGAAAACTATGTAGAATTGAGTACTGAAGAAAATAATATTGTAATTTCTTTAAGAAATTGTTCATATTATTCTTTTTTGATTCGTGTTATGTACAGGTGGTGGTGGATAATGGTATATTGCAACTAACTTTATCAAAACCTGATGGAATTATCACTGGTGTACGATATAATGGTGTTGACAATCTACTTGAAGTTCTTAATCCTGAATCTAATAGAGGGTATACACTCATCGTCTTTAATTTTTGCTATTTTTAATCCATTTCTATTAAAGAAAAACAAGTTTTCGACCTATGTATTTATTTAGTTTAATAGTTTTCGAAATTGGGTTTGGTTTTGGAAACCATAAGTAGAAGATAAATAACAAACACAAAGGAATTCATTGATTAACGTGGTATTATAACCTAAATTTTAAGCAACAAAATAGTTACTAAATGGACTACGAGCGAGTTTATTTATTTATTTTTTAAAATATTCAGGTACTGGGACCTTGTTTGGAATTCTCCAGGAAGCAAGGGAGTATTTGACGTGTATGCCTTTTCCTTCTTCACTTTTTTAATTAGTTATATGTTAGTTTTGGAATGAAAGGCCAAAATGAGCTTAGTTCGGTAGTAATTGGCAAAATGAGCTTAGTTCGGTAGTAATTGGCATGTGTTTCCTCTCTTGAAGTCGGAGCCTTGACCGTCATCTTTCCATTTTCCAAACAGCTGCTCATTTTCTCTTTTTTTTTTTTTTTTTTGCATTGGACTGGTTTTATTTATACTTTATGTTGTCATGTAATTTGAGAATCAATTGGCATGCAGGATTAAAGGGTCAAGGTTTAGGGTTATAGTGGAAAATGAGGAACAGGTTGAGCTTTCTTTTGTGAGAATGTGGGATCCCTCCCTTGAGGGCAAGTTTGTTCCTTTGAATATAGACAAAAGGTTTGCCATCAAATTTCCCATCATTGTACTAATTTTTGTTTAATTATTCACTCAATCACTTAATTGATTTTCTCTTCTAGGTTCATAGTACTACGTGGTTCTTCTGGATTTTACTCTTATGCTATTTATGAGCACTTGAAGGATTGGCCTGATTTCGATATTGGTGAAACCAGGATTACCTTTAAACTTAGAAAAGACAAGTGAGCATACTCTATCTTTACGAGTCATTTATGATTTTATCGACACGCATATAATTGTCTCCCTTTTTGTAATTACATTATCACCATCCTTGTTAATGAGGTGCCTTTTGTAGCCTTTATCCTTCACTATGAAATTCTTTTGTGTCCCATTTCATGATGTCAATGAATGGTTGCTGTTTCCAATCATCGTATCAGAGAATGCCCATTTTTTTCTTTATTTCCTTCCATACTTGCTCTTGAAATGGCCAGTAATTAGAGGACTTGGTTATCCAGTTTCTCAAGTCTCCCATAGCTTTGATATTTTCAATGTCAATAATCAATCCAATGATTGAATTGATAGGTTTCAGTACATGGCTGTAGCTGACAATAGGCAGAGAGTCATGCCCCTGCCTGATGATCGGTTATCTGGAAGATGCCAAACTTTGGCCTATCCTGAAGCTGTCTTGCTTACTAATCCCAAGAACCCTGCTCTCAAGGGTGAGGTACTCTTGGATTACTATTCTCTCTTCTGGGAATGGACTGGGATTGATCTTGAACCAGCATTTTCAATTGCAACACGGTTTTGATAGGATCCACCTTCATTTGATATGATCCTTTTTTGTTCAGGTGGATGACAAATACCAATACTCGTGTGAAAACAACAACTTAAAGGTTCATGGGTGGATAAGCACCAACCCACCCACCGGGTTTTGGCAGATTACTCCCAGTGATGAATTTCGGTCAGGTGGGCCTCTTAAGCAAAGTCTAACATCACACGTGGGACCTACAACCCTTGCTGTAGGTGATCGAACATTGCTGATCATCATTAGTTCTTTCTTTCGTGTATTTCACATCGAGTTATTATATATTCGGGTGATCTTGTGTGACAAGTAGGATTTCTAGCAAGTCTCATCTTTCTTTCTAACTTGCTCTTGTCAGATATTTCTTAGCACTCATTATGCGGGACAAGATTTGGTGCCAAAATTTAGAGCTGGCGAGCCATGGAAGAAAGTTTTTGGCCCTGTTTTTCTCTATCTTAATTCCACCTCAGTTGGAGATGACCCATTTTGGTTATGGGAGGATGCTAAAATACAGGTTTGATGCATTAAGACTCTCAGTGACTCAACTTTTTTCTTTGTGTGGATGACACTTCGACCTAGAAATACTTATAATCATATGACTATCAGATGATGGCAGAAGTCGATAGTTGGCCATATAGTTTCCCTGCATCAGAGGATTTCCCAAAAAAAGATCAACGGGGCATCGTTAGTGGCAGGCTACTGGTTTTTGATAGGTAAGCATTTCTTATTTGTAAATTCTTGGTAAGAACTGATACAATGATGTGGTTTTTTATCAAATTTCATTTGGCCAATATCTTTCATTATATTTTCGTAAAGAGCTTAAAAGAGGATGTATCTGCTATGTGAAAATTTCATGACTCCTTCCAAGTTTCAGCTGCAAGATGTAGTAAAGATGGTAATCACTTATCAATATTAAAAAATCTGTAGCAGATAATCAAACGTAGAAAAACAGGTTTTCTACAGTAACCAAAATTTGTTGCTTTTGATTCATAATTATTATTTTCTATTTTATTTTTATGATTTCTGCAAAAGTAAAAATGAAGGTTCAGAAAATGTTGTTAAGTTAGAAATCTGGAAATTGAATTCAGGGACCCACATTCCTATATTCGTTAGCCTTAAACGGATTTAGAGATGTTTCTTGCACAAAGAAAAAAAAGGAATATACTGACTTTTCTAAAATTTGAAATGAAAATGATAAACAAGAAACAGCATATTATTAATTGGATTCTCTACTTGTCTTATCTTTTAGTTTAATTTATGTGCTTGGACTTTTGTTTTTAAAATATCTAGATATGTGGATCAAGACTATCTTCCAGCCTGTGGAGCCTTCGTCGGATTGTCTGCACCAGGAGAAGCGGGATCATGGCAGAGAGAATGCAAGGTAACTAACTTTGTTTCCAGAGATTGTCTACTATCGTCTGCCTTGTCAGTGTTTTTCTAAAGCTCGAGGTAGTATGAAGCGCAATAGTCTTCCAGAACTTTAAGACAAGGTGAAAAAAAAAGGCGAGTACATATGAAATGCTCTATATATAAAGGCACGACATGAAGAAGAGAAAAAATAGTTGAAGTGGGAATATGAAAAAAAACCGCAAACACGTGGATTGATATATTAATTAGTAAGAAGGAAAAACCAAAGTTATTATTATATTTTCTAAGATAATGAAAAAGCCAGGCGCCAGACTCCGTCTAGCATCTAGGCACACGCTTGATAGGGCTTTTAAAAACATTGACCTTGGCCTAGTGAAGGTCAAACTTGTAGTTCAAAAATTATTTCAGCATACAGAATTTTCATTACTAGGATACCGTACCTGCAACCTATGGTTTATGATGACTTTTTTTTTCTCTCTCTCTCAGAAATTTCTTTGTTGATATTGATATTCATTGTCTTTTAAATTCAAAAGCAAAGCTATCAAATCTGAAGGGATACAATATTATGCACATGTGTTATATTTCTGGAATTTGAACAATTTTCAGAACTTCCAAATCTTTTTCGTACCTATGAAAGCGTGGTAAAGCAGCAGAAGAATAGATAGACATTGTCTTATCGACTTTTATTAAAAATACAACGTTTGGAGAGGACTGAAGGTTTAGTCTCTCGAATGAACTAGAAATGACTTCTGATTTGTACTTGCAGATTGCCCTTCCTTGTAGATATAATTCCCCAACCTTTAAACAAGTTAAATAACTTCACATTTCTTTTCTGCCTTCAGTATAAGTAACGACTTATAGCAACATACTATCCTGCCCTTCTCTCAGAGACCACCATTGACTCTCGAAGATCTAACCCTCTTTAGTGGCACAAATCCTCCTCGTAGAATTATAAAATGAATAGAGTCTCATGTTTCTATCAAGATGGTATCAAAAAAATTAGAGAAGCTATAATGATATGGTGAAAGGATGAGGATAAAACTAATTTTGTTTGATTCTTTCATCAATTTCATCGAAGTCCTTGAAAAGAAATGGAAAATACTGAATACTTGAGCTTTTAGAACTACATTTTAACTCACATGGAAGCCTTCTAGCTTCATTCGATTATGAGAACTTTTCGTTAATAGTATCTGTAAGGTTAAATCATATGTATCCTTTAAAAGAGTTTATTCTTATTGTCAGGGATATCAATTCTGGACTAGAGCAGATGATGGTGGTTATTTTACCATCAACCACGTACACACCGGTGAGTATAATCTATATGCATGGGTTCCTGGCTTCATTGGAGATTATCGCTGTGATGCCCTCATCAATGTAAGCCAAGGTACAGTTCCCTCTTCCCTGTATTGCATAATAAATTGACTGAAGCCTTCAATTTTTAAGTGCACAGTTACAGAATTCCAAGTTGATATGTAGGATCTTGCATTGAAATGGGTGATCTTGTATATGAACCTCCTAGAGATGGGCCTACGCTTTGGGAAATAGGCATTCCCGATCGTTCTGCATCCGAGTTCTATGTTCCTGATCCTAATCCAATACATATTAATAAACTCTTTGTTGACCATCCTGACAGGTACTCCCTTTCTATAGTTCTAACTTCTATTAATTAAATGAACTTTGACATTATGGTCCGTTTTTACAGTCTGTTTCACACTGTTTCCATATAGAAATTTGAGTTCTTATTCTGAGGATGGGAATGTAAAAGTGTCTGTCGATATCCCTTGTTATAGAATGAAGATGACAGTAAGAATCCCAGCTAGGAAAATATTAGGATTATTAGTATGATAAGTAAGAAAGCATCTTCTAGTAATTAAACAATAATGAAATTTCTTATGTTGAGCGTTTTGATGCTTGGGGGAGGATACTTAACCCTTTAGAATGTGTTGAAAAGCGTTGGTTCTTTATTTCTATCACTTGTAATTGTTTTTCTTATCAACATTAATGTCAGTGGATTTTCCTTGAAGTAGCTATATCACATGATGTTTGCATTGTGATTTCTTGGGTATTTGTGTTCTTTATTAGAATTTTCTGTTTGTGGAAGAAGAATCTTAACATTAACAATGACTTATGTAGATCATGAGTGATTCAAATCCAACTAAAGCTGAACTCTCCAATGTTTAACACTTATACTCTGCAGGTTCCGGCAGTACGGGTTGTGGAACAGATACGCTGAATTATACCCGAATGAGGATTTAGTATATACAGTTGGCACAAGCGACTATAGAAAAGATTGGTTCTTTGCTCATGTCCCTAGGTAACATTTCTTTGGCTCTTCATTGTCACGACCAAGAACAACAATGGCGATTGTTGTCGTCAGACCGAACAAACAATTTCTCCTCTTCATCAATGAATATGGTCGTTATCATTATATGGTCATTAACATGTCTTGTATTTGTCATCAAACTATTCCATTTATTGCAGGAAGACAAACAATTCACATGAAGGAACTACTTGGCAAATCAAATTCAAACTTGATAGAGTGGATCACAAGAGTATGTATACACTACGGGTTGCAATTGCTTCTGCTACTCTTGCTGAATTGCAGGTATTATGCTGTACGATTAGCCATAACTGAAATGCTCGACATTTGAATTTAGTCTCTGTACTTTAACGATTGCATCTATATGGTTTTCGAAATTTTAAAGTTACAAATTTATTCCTTGAACTCTCGAATTCTTTGAAAATTCATAGATTTACTCAACAAAATTGAAAATTAGAGCTTACTAACGTAAAATTCAAATTATATCTAATAGAAAAGTTTATTTTTGTATCTTTTGAATTGGTTAGAGTTCTACTAGGCACAAATTAAAAAAATTTTGAGGTTGTTTTGGGAGTGAGCTCCATGTTCGAGACTCCAAAATATAGCAGTGGTGTTTACTATTGCTTGTAGTTTATAATTATATACAACAATGCTATTTGAAACATCTCTTTAATGTTGTGTTTACTACTTTCTACCCAAACTAAAATAGTTTACACCTCAAACAGAGACTATTATAATTTATAGACTATACTAACCAACTTAACGTCCCAAATGCTCCCTTATACATAAAGGGACCTGTTAAAATTTTAAACCACCTTGAAAGTTGTAAAGATGAGATATACGCAAACTTCAAAGTTAAGGACTAAACTTATAATTTAACCTTAATCTATTTTTCCACTTCTCTCTCTGCTCTCACCCTCTAGGTGCGGGTAAATGATCCGAGATCGAAGCGACCTTTGTTTACGAGCGGGTTGATAGGGAGGGACAATTCAATTGCAAGACATGGAATTCATGGAATTTATTGGGTTTATGGTGTGGGCGTAGCTGGGTGTCATCTTGTTGAAGGAGAAAACACAATCTTCTTGACTCAGCCACGCTGCACCAGCCCTTTTCAGGGTATCATGTATGATTATATCCGTCTTGAAGGTCCTCCAACAAAGTGATTAATTCTTTCTTTACCTAACATTTTATATATTGCACTTCTTTTTTCTCAAAAAGTTAAATTTCCTATTTCGTATTATGTTTGTGTATGAATTACTTGTTTTCGAGAGATATTGTTCCTTTTAACTCTTTATGATCTTTTTTCAGAAGTGATTTTTTAACCTTTGTTTGGTAACAATTTCGTTTTTT

Coding sequence (CDS)

ATGTCATCCAGAGCCGTCCGCTTGCAGATACGAGATCATCATGTGGTGGTGGATAATGGTATATTGCAACTAACTTTATCAAAACCTGATGGAATTATCACTGGTGTACGATATAATGGTGTTGACAATCTACTTGAAGTTCTTAATCCTGAATCTAATAGAGGGATTAAAGGGTCAAGGTTTAGGGTTATAGTGGAAAATGAGGAACAGGTTGAGCTTTCTTTTGTGAGAATGTGGGATCCCTCCCTTGAGGGCAAGTTTGTTCCTTTGAATATAGACAAAAGGTTCATAGTACTACGTGGTTCTTCTGGATTTTACTCTTATGCTATTTATGAGCACTTGAAGGATTGGCCTGATTTCGATATTGGTGAAACCAGGATTACCTTTAAACTTAGAAAAGACAAGTTTCAGTACATGGCTGTAGCTGACAATAGGCAGAGAGTCATGCCCCTGCCTGATGATCGGTTATCTGGAAGATGCCAAACTTTGGCCTATCCTGAAGCTGTCTTGCTTACTAATCCCAAGAACCCTGCTCTCAAGGGTGAGGTGGATGACAAATACCAATACTCGTGTGAAAACAACAACTTAAAGGTTCATGGGTGGATAAGCACCAACCCACCCACCGGGTTTTGGCAGATTACTCCCAGTGATGAATTTCGGTCAGGTGGGCCTCTTAAGCAAAGTCTAACATCACACGTGGGACCTACAACCCTTGCTATATTTCTTAGCACTCATTATGCGGGACAAGATTTGGTGCCAAAATTTAGAGCTGGCGAGCCATGGAAGAAAGTTTTTGGCCCTGTTTTTCTCTATCTTAATTCCACCTCAGTTGGAGATGACCCATTTTGGTTATGGGAGGATGCTAAAATACAGATGATGGCAGAAGTCGATAGTTGGCCATATAGTTTCCCTGCATCAGAGGATTTCCCAAAAAAAGATCAACGGGGCATCGTTAGTGGCAGGCTACTGGTTTTTGATAGATATGTGGATCAAGACTATCTTCCAGCCTGTGGAGCCTTCGTCGGATTGTCTGCACCAGGAGAAGCGGGATCATGGCAGAGAGAATGCAAGGGATATCAATTCTGGACTAGAGCAGATGATGGTGGTTATTTTACCATCAACCACGTACACACCGGTGAGTATAATCTATATGCATGGGTTCCTGGCTTCATTGGAGATTATCGCTGTGATGCCCTCATCAATGTAAGCCAAGGATCTTGCATTGAAATGGGTGATCTTGTATATGAACCTCCTAGAGATGGGCCTACGCTTTGGGAAATAGGCATTCCCGATCGTTCTGCATCCGAGTTCTATGTTCCTGATCCTAATCCAATACATATTAATAAACTCTTTGTTGACCATCCTGACAGGTTCCGGCAGTACGGGTTGTGGAACAGATACGCTGAATTATACCCGAATGAGGATTTAGTATATACAGTTGGCACAAGCGACTATAGAAAAGATTGGTTCTTTGCTCATGTCCCTAGGAAGACAAACAATTCACATGAAGGAACTACTTGGCAAATCAAATTCAAACTTGATAGAGTGGATCACAAGAGTATGTATACACTACGGGTTGCAATTGCTTCTGCTACTCTTGCTGAATTGCAGGTGCGGGTAAATGATCCGAGATCGAAGCGACCTTTGTTTACGAGCGGGTTGATAGGGAGGGACAATTCAATTGCAAGACATGGAATTCATGGAATTTATTGGGTTTATGGTGTGGGCGTAGCTGGGTGTCATCTTGTTGAAGGAGAAAACACAATCTTCTTGACTCAGCCACGCTGCACCAGCCCTTTTCAGGGTATCATGTATGATTATATCCGTCTTGAAGGTCCTCCAACAAAGTGA

Protein sequence

MSSRAVRLQIRDHHVVVDNGILQLTLSKPDGIITGVRYNGVDNLLEVLNPESNRGIKGSRFRVIVENEEQVELSFVRMWDPSLEGKFVPLNIDKRFIVLRGSSGFYSYAIYEHLKDWPDFDIGETRITFKLRKDKFQYMAVADNRQRVMPLPDDRLSGRCQTLAYPEAVLLTNPKNPALKGEVDDKYQYSCENNNLKVHGWISTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTLAIFLSTHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSVGDDPFWLWEDAKIQMMAEVDSWPYSFPASEDFPKKDQRGIVSGRLLVFDRYVDQDYLPACGAFVGLSAPGEAGSWQRECKGYQFWTRADDGGYFTINHVHTGEYNLYAWVPGFIGDYRCDALINVSQGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFYVPDPNPIHINKLFVDHPDRFRQYGLWNRYAELYPNEDLVYTVGTSDYRKDWFFAHVPRKTNNSHEGTTWQIKFKLDRVDHKSMYTLRVAIASATLAELQVRVNDPRSKRPLFTSGLIGRDNSIARHGIHGIYWVYGVGVAGCHLVEGENTIFLTQPRCTSPFQGIMYDYIRLEGPPTK

Homology

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BLAST of Cucsat.G12992 vs. ExPASy Swiss-Prot
Match: A5ABH4 (Probable rhamnogalacturonate lyase B OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=rglB PE=3 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 8.3e-18
Identity = 97/377 (25.73%), Postives = 152/377 (40.32%), Query Frame = 0


Query: 32  KYQYSCENNNLKVHGW-----ISTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTLAI 91
           KY +S    +  VHG       S     G W +  + +   GGPL   LT  V       
Sbjct: 216 KYTFSNAWRDNSVHGMYADGSTSNGSTFGAWLVMNTKDTYYGGPLHSDLT--VDGIVYNY 275

Query: 92  FLSTHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSVGDDPFWLWEDAKIQMMAEVD-SW 151
            +S H+   +  P    G  + + FGP + + N    G       ++ K       D SW
Sbjct: 276 LVSNHHG--EGTPNITYG--FDRTFGPQYYHFNG---GKGSTASLQELKSDAETLADPSW 335

Query: 152 PYSFPAS-----EDFPKKDQRGIVSGRLLVFDRYVDQDYLPACGAFVGLSAPGEAGSWQR 211
              F  S       +    QRG V G++           LP  GA   ++     G + +
Sbjct: 336 NVDFYDSIAKHVVGYTPSSQRGSVQGKI----------KLPK-GATRPIAVLTVDGQYFQ 395

Query: 212 E----CKGYQFWTRADDGGYFTINHVHTGEYNLYAWVPGFIGDYRCDALINVSQGSCIEM 271
           +       YQ+W   DD G+F+++HV  G Y L  +  G  GD+  D  + V  G    +
Sbjct: 396 DNSVNSSSYQYWAEIDDSGHFSVDHVKEGPYRLTVYADGIFGDFVRDG-VQVKAGKKTTI 455

Query: 272 GDLVYEPPRDGPTLWEIGIPDRSASEF---YVPDP-NPIHINKLFVDHPDRFRQYGLWNR 331
            +  +E    G  +W +G PD+S+ EF      DP +P+H        P+    +G ++ 
Sbjct: 456 QE-TWEAESAGTEIWRLGTPDKSSGEFRHGVARDPTHPLH-------PPEYLIYWGAYDW 515

Query: 332 YAELYPNEDLVYTVGTSDYRKD-----W-FFAHVPRKTNNSHEGT-TWQIKFKL--DRVD 381
            ++ +P + + YT+GTSD   D     W  F   P      ++ T  W I F L  D + 
Sbjct: 516 QSD-FP-DGINYTIGTSDPATDLNTVHWSVFGPTPNDPRVEYDTTHDWTINFPLSEDDLA 561

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BLAST of Cucsat.G12992 vs. ExPASy Swiss-Prot
Match: Q5AZ85 (Rhamnogalacturonate lyase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=rglB PE=2 SV=2)

HSP 1 Score: 79.3 bits (194), Expect = 1.2e-13
Identity = 116/474 (24.47%), Postives = 175/474 (36.92%), Query Frame = 0


Query: 32  KYQYSCENNNLKVHGWI---STNPPT--GFWQITPSDEFRSGGPLKQSLTSHVGPTTLAI 91
           KY +S    +  VHG     ST+  T  G W +  + +   GGPL   LT  V       
Sbjct: 215 KYTFSNHWRDNDVHGLYADGSTSDGTTYGAWLVMNTKDTYYGGPLHSDLT--VDGIIYNY 274

Query: 92  FLSTHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSVGDDPFWLWEDAKIQMMAEVDSWP 151
            +S H+   +  P    G  + + FGP F   N    G     L E           SW 
Sbjct: 275 IVSNHHG--EGTPNITNG--FDRTFGPQFYLFN----GGGSSSLEELRDEARSLASPSWN 334

Query: 152 YSFPAS-----EDFPKKDQRGIVSGRLLVFDRYVDQDYLP--ACGAFVGLSAPGEAGSWQ 211
             F  S       +    QRG V G +           LP  A      L+  G      
Sbjct: 335 ADFYDSIAKHVIGYVPSSQRGSVKGTI----------KLPKNAKSPIAVLTVDGHYFQDN 394

Query: 212 REC-KGYQFWTRADDGGYFTINHVHTGEYNLYAWVPGFIGDYRCDALINVSQGSCIEMGD 271
                 +Q+W   D  G F+I+ V  G+Y L  +  G  GD+  D ++  ++ S      
Sbjct: 395 SAVPSSHQYWADIDKNGRFSIDRVVAGKYRLTVYADGIFGDFTRDGIVVKARKSTSIKE- 454

Query: 272 LVYEPPRDGPTLWEIGIPDRSASEF-YVPDPNPIHINKLFVDHPDRFRQYGLWNRY--AE 331
             ++P   G  +W +G PD+S+ EF +    +P H       HP  +  Y  W  Y    
Sbjct: 455 -TWKPESAGTEIWRLGTPDKSSGEFRHGAARDPTHPR-----HPPEYLIY--WGAYDWQS 514

Query: 332 LYPNEDLVYTVGTSDYRKDWFFAH----VPRKTNNSHEGTT---WQIKFKLD--RVDHKS 391
            +P   + Y +G SD   D+   H     P   N   E  T   W+I+F L   ++  + 
Sbjct: 515 DFPG-GIDYMIGESDPATDFNTVHWAVFGPTPDNPVAESNTTHDWRIRFDLSAKQLHARK 574

Query: 392 MYTLRVAIASATLAELQVRV---NDPRSKRPLFT---------SGLIGRDNS---IARHG 451
             TL + +A A  A     V   ++P +  PL +         + +IG D S   I R  
Sbjct: 575 TATLTIQLAGAKAASGNTDVYNASEPYANLPLRSYINEQEEPLTMVIGYDQSSSCIVRSA 634

Query: 452 IHGIYWVYGVGVAGCHLVEGENTIFLTQPRCTSPFQG--------IMYDYIRLE 458
           +               L EG N + L+ P   + ++         + YD +RLE
Sbjct: 635 VSCYQVREKWEFPASWLKEGSNLLRLSLPTNGTNYESAVLPTSVYVQYDALRLE 658

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BLAST of Cucsat.G12992 vs. NCBI nr
Match: XP_031739419.1 (probable rhamnogalacturonate lyase B isoform X2 [Cucumis sativus])

HSP 1 Score: 943 bits (2437), Expect = 0.0
Identity = 435/438 (99.32%), Postives = 435/438 (99.32%), Query Frame = 0


Query: 25  LLSRVDDKYQYSCENNNLKVHGWISTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTL 84
           L   VDDKYQYSCENNNLKVHGWISTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTL
Sbjct: 179 LKGEVDDKYQYSCENNNLKVHGWISTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTL 238

Query: 85  AIFLSTHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSVGDDPFWLWEDAKIQMMAEVDS 144
           AIFLSTHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSVGDDPFWLWEDAKIQMMAEVDS
Sbjct: 239 AIFLSTHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSVGDDPFWLWEDAKIQMMAEVDS 298

Query: 145 WPYSFPASEDFPKKDQRGIVSGRLLVFDRYVDQDYLPACGAFVGLSAPGEAGSWQRECKG 204
           WPYSFPASEDFPKKDQRGIVSGRLLVFDRYVDQDYLPACGAFVGLSAPGEAGSWQRECKG
Sbjct: 299 WPYSFPASEDFPKKDQRGIVSGRLLVFDRYVDQDYLPACGAFVGLSAPGEAGSWQRECKG 358

Query: 205 YQFWTRADDGGYFTINHVHTGEYNLYAWVPGFIGDYRCDALINVSQGSCIEMGDLVYEPP 264
           YQFWTRADDGGYFTINHVHTGEYNLYAWVPGFIGDYRCDALINVSQGSCIEMGDLVYEPP
Sbjct: 359 YQFWTRADDGGYFTINHVHTGEYNLYAWVPGFIGDYRCDALINVSQGSCIEMGDLVYEPP 418

Query: 265 RDGPTLWEIGIPDRSASEFYVPDPNPIHINKLFVDHPDRFRQYGLWNRYAELYPNEDLVY 324
           RDGPTLWEIGIPDRSASEFYVPDPNPIHINKLFVDHPDRFRQYGLWNRYAELYPNEDLVY
Sbjct: 419 RDGPTLWEIGIPDRSASEFYVPDPNPIHINKLFVDHPDRFRQYGLWNRYAELYPNEDLVY 478

Query: 325 TVGTSDYRKDWFFAHVPRKTNNSHEGTTWQIKFKLDRVDHKSMYTLRVAIASATLAELQV 384
           TVGTSDYRKDWFFAHVPRKTNNSHEGTTWQIKFKLDRVDHKSMYTLRVAIASATLAELQV
Sbjct: 479 TVGTSDYRKDWFFAHVPRKTNNSHEGTTWQIKFKLDRVDHKSMYTLRVAIASATLAELQV 538

Query: 385 RVNDPRSKRPLFTSGLIGRDNSIARHGIHGIYWVYGVGVAGCHLVEGENTIFLTQPRCTS 444
           RVNDPRSKRPLFTSGLIGRDNSIARHGIHGIYWVYGVGVAGCHLVEGENTIFLTQPRCTS
Sbjct: 539 RVNDPRSKRPLFTSGLIGRDNSIARHGIHGIYWVYGVGVAGCHLVEGENTIFLTQPRCTS 598

Query: 445 PFQGIMYDYIRLEGPPTK 462
           PFQGIMYDYIRLEGPPTK
Sbjct: 599 PFQGIMYDYIRLEGPPTK 616

[Close]

BLAST of Cucsat.G12992 vs. NCBI nr
Match: XP_004136250.1 (probable rhamnogalacturonate lyase B isoform X1 [Cucumis sativus] >KGN60276.1 hypothetical protein Csa_001404 [Cucumis sativus])

HSP 1 Score: 943 bits (2437), Expect = 0.0
Identity = 435/438 (99.32%), Postives = 435/438 (99.32%), Query Frame = 0


Query: 25  LLSRVDDKYQYSCENNNLKVHGWISTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTL 84
           L   VDDKYQYSCENNNLKVHGWISTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTL
Sbjct: 196 LKGEVDDKYQYSCENNNLKVHGWISTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTL 255

Query: 85  AIFLSTHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSVGDDPFWLWEDAKIQMMAEVDS 144
           AIFLSTHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSVGDDPFWLWEDAKIQMMAEVDS
Sbjct: 256 AIFLSTHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSVGDDPFWLWEDAKIQMMAEVDS 315

Query: 145 WPYSFPASEDFPKKDQRGIVSGRLLVFDRYVDQDYLPACGAFVGLSAPGEAGSWQRECKG 204
           WPYSFPASEDFPKKDQRGIVSGRLLVFDRYVDQDYLPACGAFVGLSAPGEAGSWQRECKG
Sbjct: 316 WPYSFPASEDFPKKDQRGIVSGRLLVFDRYVDQDYLPACGAFVGLSAPGEAGSWQRECKG 375

Query: 205 YQFWTRADDGGYFTINHVHTGEYNLYAWVPGFIGDYRCDALINVSQGSCIEMGDLVYEPP 264
           YQFWTRADDGGYFTINHVHTGEYNLYAWVPGFIGDYRCDALINVSQGSCIEMGDLVYEPP
Sbjct: 376 YQFWTRADDGGYFTINHVHTGEYNLYAWVPGFIGDYRCDALINVSQGSCIEMGDLVYEPP 435

Query: 265 RDGPTLWEIGIPDRSASEFYVPDPNPIHINKLFVDHPDRFRQYGLWNRYAELYPNEDLVY 324
           RDGPTLWEIGIPDRSASEFYVPDPNPIHINKLFVDHPDRFRQYGLWNRYAELYPNEDLVY
Sbjct: 436 RDGPTLWEIGIPDRSASEFYVPDPNPIHINKLFVDHPDRFRQYGLWNRYAELYPNEDLVY 495

Query: 325 TVGTSDYRKDWFFAHVPRKTNNSHEGTTWQIKFKLDRVDHKSMYTLRVAIASATLAELQV 384
           TVGTSDYRKDWFFAHVPRKTNNSHEGTTWQIKFKLDRVDHKSMYTLRVAIASATLAELQV
Sbjct: 496 TVGTSDYRKDWFFAHVPRKTNNSHEGTTWQIKFKLDRVDHKSMYTLRVAIASATLAELQV 555

Query: 385 RVNDPRSKRPLFTSGLIGRDNSIARHGIHGIYWVYGVGVAGCHLVEGENTIFLTQPRCTS 444
           RVNDPRSKRPLFTSGLIGRDNSIARHGIHGIYWVYGVGVAGCHLVEGENTIFLTQPRCTS
Sbjct: 556 RVNDPRSKRPLFTSGLIGRDNSIARHGIHGIYWVYGVGVAGCHLVEGENTIFLTQPRCTS 615

Query: 445 PFQGIMYDYIRLEGPPTK 462
           PFQGIMYDYIRLEGPPTK
Sbjct: 616 PFQGIMYDYIRLEGPPTK 633

[Close]

BLAST of Cucsat.G12992 vs. NCBI nr
Match: XP_008466137.1 (PREDICTED: probable rhamnogalacturonate lyase B isoform X1 [Cucumis melo] >KAA0038653.1 putative rhamnogalacturonate lyase B isoform X1 [Cucumis melo var. makuwa] >TYK31256.1 putative rhamnogalacturonate lyase B isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 898 bits (2321), Expect = 0.0
Identity = 416/439 (94.76%), Postives = 425/439 (96.81%), Query Frame = 0


Query: 25  LLSRVDDKYQYSCENNNLKVHGWISTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTL 84
           L   VDDKYQYSCENNNLKVHGWISTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTL
Sbjct: 196 LKGEVDDKYQYSCENNNLKVHGWISTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTL 255

Query: 85  AIFLSTHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSVGDDPFWLWEDAKIQMMAEVDS 144
           AIFLS HYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSVGDDPFWLWEDAKIQMMAEV+S
Sbjct: 256 AIFLSAHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSVGDDPFWLWEDAKIQMMAEVES 315

Query: 145 WPYSFPASEDFPKKDQRGIVSGRLLVFDRYVDQDYLPACGAFVGLSAPGEAGSWQRECKG 204
           WPYSFPASEDF KKDQRG+VSGRLLVFDRYVDQDYLP  GAFVGLSAPGE GSWQRECKG
Sbjct: 316 WPYSFPASEDFSKKDQRGMVSGRLLVFDRYVDQDYLPGSGAFVGLSAPGEVGSWQRECKG 375

Query: 205 YQFWTRADDGGYFTINHVHTGEYNLYAWVPGFIGDYRCDALINVSQGSCIEMGDLVYEPP 264
           YQFWTRADDGGYFTINHV TG+YNLYAWVPGFIGDYRCDAL+NVSQGSCIEMGDLVYEPP
Sbjct: 376 YQFWTRADDGGYFTINHVLTGDYNLYAWVPGFIGDYRCDALVNVSQGSCIEMGDLVYEPP 435

Query: 265 RDGPTLWEIGIPDRSASEFYVPDPNPIHINKLFVDHPDRFRQYGLWNRYAELYPNEDLVY 324
           RDGPTLWEIGIPDRSASEFYVPDPNPI+INKLFV+HPDRFRQYGLW+RYAELYP+EDLVY
Sbjct: 436 RDGPTLWEIGIPDRSASEFYVPDPNPIYINKLFVNHPDRFRQYGLWDRYAELYPDEDLVY 495

Query: 325 TVGTSDYRKDWFFAHVPRKTN-NSHEGTTWQIKFKLDRVDHKSMYTLRVAIASATLAELQ 384
           TVGTSDYRKDWFFAHVPRKTN NSHEGTTWQIKF+LDRVD KSMY LRVAIASATLAELQ
Sbjct: 496 TVGTSDYRKDWFFAHVPRKTNSNSHEGTTWQIKFQLDRVDRKSMYMLRVAIASATLAELQ 555

Query: 385 VRVNDPRSKRPLFTSGLIGRDNSIARHGIHGIYWVYGVGVAGCHLVEGENTIFLTQPRCT 444
           VRVNDPRSKRPLFTSGLIGRDNSIARHGIHGIYWVY V VAGCHLVEGENTIFLTQPRCT
Sbjct: 556 VRVNDPRSKRPLFTSGLIGRDNSIARHGIHGIYWVYSVSVAGCHLVEGENTIFLTQPRCT 615

Query: 445 SPFQGIMYDYIRLEGPPTK 462
           SPFQGIMYDYIRLEGPPTK
Sbjct: 616 SPFQGIMYDYIRLEGPPTK 634

[Close]

BLAST of Cucsat.G12992 vs. NCBI nr
Match: XP_008466138.1 (PREDICTED: probable rhamnogalacturonate lyase B isoform X2 [Cucumis melo])

HSP 1 Score: 898 bits (2321), Expect = 0.0
Identity = 416/439 (94.76%), Postives = 425/439 (96.81%), Query Frame = 0


Query: 25  LLSRVDDKYQYSCENNNLKVHGWISTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTL 84
           L   VDDKYQYSCENNNLKVHGWISTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTL
Sbjct: 102 LKGEVDDKYQYSCENNNLKVHGWISTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTL 161

Query: 85  AIFLSTHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSVGDDPFWLWEDAKIQMMAEVDS 144
           AIFLS HYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSVGDDPFWLWEDAKIQMMAEV+S
Sbjct: 162 AIFLSAHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSVGDDPFWLWEDAKIQMMAEVES 221

Query: 145 WPYSFPASEDFPKKDQRGIVSGRLLVFDRYVDQDYLPACGAFVGLSAPGEAGSWQRECKG 204
           WPYSFPASEDF KKDQRG+VSGRLLVFDRYVDQDYLP  GAFVGLSAPGE GSWQRECKG
Sbjct: 222 WPYSFPASEDFSKKDQRGMVSGRLLVFDRYVDQDYLPGSGAFVGLSAPGEVGSWQRECKG 281

Query: 205 YQFWTRADDGGYFTINHVHTGEYNLYAWVPGFIGDYRCDALINVSQGSCIEMGDLVYEPP 264
           YQFWTRADDGGYFTINHV TG+YNLYAWVPGFIGDYRCDAL+NVSQGSCIEMGDLVYEPP
Sbjct: 282 YQFWTRADDGGYFTINHVLTGDYNLYAWVPGFIGDYRCDALVNVSQGSCIEMGDLVYEPP 341

Query: 265 RDGPTLWEIGIPDRSASEFYVPDPNPIHINKLFVDHPDRFRQYGLWNRYAELYPNEDLVY 324
           RDGPTLWEIGIPDRSASEFYVPDPNPI+INKLFV+HPDRFRQYGLW+RYAELYP+EDLVY
Sbjct: 342 RDGPTLWEIGIPDRSASEFYVPDPNPIYINKLFVNHPDRFRQYGLWDRYAELYPDEDLVY 401

Query: 325 TVGTSDYRKDWFFAHVPRKTN-NSHEGTTWQIKFKLDRVDHKSMYTLRVAIASATLAELQ 384
           TVGTSDYRKDWFFAHVPRKTN NSHEGTTWQIKF+LDRVD KSMY LRVAIASATLAELQ
Sbjct: 402 TVGTSDYRKDWFFAHVPRKTNSNSHEGTTWQIKFQLDRVDRKSMYMLRVAIASATLAELQ 461

Query: 385 VRVNDPRSKRPLFTSGLIGRDNSIARHGIHGIYWVYGVGVAGCHLVEGENTIFLTQPRCT 444
           VRVNDPRSKRPLFTSGLIGRDNSIARHGIHGIYWVY V VAGCHLVEGENTIFLTQPRCT
Sbjct: 462 VRVNDPRSKRPLFTSGLIGRDNSIARHGIHGIYWVYSVSVAGCHLVEGENTIFLTQPRCT 521

Query: 445 SPFQGIMYDYIRLEGPPTK 462
           SPFQGIMYDYIRLEGPPTK
Sbjct: 522 SPFQGIMYDYIRLEGPPTK 540

[Close]

BLAST of Cucsat.G12992 vs. NCBI nr
Match: XP_038897922.1 (probable rhamnogalacturonate lyase B [Benincasa hispida])

HSP 1 Score: 874 bits (2257), Expect = 0.0
Identity = 402/439 (91.57%), Postives = 414/439 (94.31%), Query Frame = 0


Query: 25  LLSRVDDKYQYSCENNNLKVHGWISTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTL 84
           L   VDDKYQYSCEN +LKVHGWI TNPPTGFW ITPSDEFRSGGPLKQSLTSHVGPTTL
Sbjct: 196 LKGEVDDKYQYSCENKDLKVHGWICTNPPTGFWHITPSDEFRSGGPLKQSLTSHVGPTTL 255

Query: 85  AIFLSTHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSVGDDPFWLWEDAKIQMMAEVDS 144
           AIFLS HYAGQDLVPKFRAGEPWKKVFGPVF YLNSTSVGDDPFWLWEDAKIQMMAEV+S
Sbjct: 256 AIFLSAHYAGQDLVPKFRAGEPWKKVFGPVFFYLNSTSVGDDPFWLWEDAKIQMMAEVES 315

Query: 145 WPYSFPASEDFPKKDQRGIVSGRLLVFDRYVDQDYLPACGAFVGLSAPGEAGSWQRECKG 204
           WPYSFPASEDFPK DQRG+V GRLLV DRYV QDYLPA GAFVG+SAPGE GSWQRECKG
Sbjct: 316 WPYSFPASEDFPKVDQRGVVRGRLLVLDRYVSQDYLPASGAFVGISAPGEVGSWQRECKG 375

Query: 205 YQFWTRADDGGYFTINHVHTGEYNLYAWVPGFIGDYRCDALINVSQGSCIEMGDLVYEPP 264
           YQFWTRADDGGYFTINH HTG+YNLY+WVPGFIGDYRCDALINV+QGSCIEMGDLVYEPP
Sbjct: 376 YQFWTRADDGGYFTINHAHTGDYNLYSWVPGFIGDYRCDALINVNQGSCIEMGDLVYEPP 435

Query: 265 RDGPTLWEIGIPDRSASEFYVPDPNPIHINKLFVDHPDRFRQYGLWNRYAELYPNEDLVY 324
           RDGPTLWEIGIPDRSASEFYVPDPNP HINKLFV+HPDRFRQYGLW+RYAELYPNEDLVY
Sbjct: 436 RDGPTLWEIGIPDRSASEFYVPDPNPTHINKLFVNHPDRFRQYGLWDRYAELYPNEDLVY 495

Query: 325 TVGTSDYRKDWFFAHVPRKT-NNSHEGTTWQIKFKLDRVDHKSMYTLRVAIASATLAELQ 384
           TVGTSDY KDWFFA VPRKT +NSHEGTTWQIKF L+RV  KSMYTLRVAIASATLAELQ
Sbjct: 496 TVGTSDYSKDWFFAQVPRKTKSNSHEGTTWQIKFYLNRVARKSMYTLRVAIASATLAELQ 555

Query: 385 VRVNDPRSKRPLFTSGLIGRDNSIARHGIHGIYWVYGVGVAGCHLVEGENTIFLTQPRCT 444
           VRVNDPRSKRPLFTSGLIGRDNSIARHGIHGIYW+Y VGV GCHLVEGENTIFLTQPRCT
Sbjct: 556 VRVNDPRSKRPLFTSGLIGRDNSIARHGIHGIYWLYSVGVGGCHLVEGENTIFLTQPRCT 615

Query: 445 SPFQGIMYDYIRLEGPPTK 462
           SPFQGIMYDYIRLEGPPT 
Sbjct: 616 SPFQGIMYDYIRLEGPPTN 634

[Close]

BLAST of Cucsat.G12992 vs. ExPASy TrEMBL
Match: A0A0A0LJQ9 (Rhamnogalacturonan endolyase OS=Cucumis sativus OX=3659 GN=Csa_3G892770 PE=3 SV=1)

HSP 1 Score: 943 bits (2437), Expect = 0.0
Identity = 435/438 (99.32%), Postives = 435/438 (99.32%), Query Frame = 0


Query: 25  LLSRVDDKYQYSCENNNLKVHGWISTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTL 84
           L   VDDKYQYSCENNNLKVHGWISTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTL
Sbjct: 196 LKGEVDDKYQYSCENNNLKVHGWISTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTL 255

Query: 85  AIFLSTHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSVGDDPFWLWEDAKIQMMAEVDS 144
           AIFLSTHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSVGDDPFWLWEDAKIQMMAEVDS
Sbjct: 256 AIFLSTHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSVGDDPFWLWEDAKIQMMAEVDS 315

Query: 145 WPYSFPASEDFPKKDQRGIVSGRLLVFDRYVDQDYLPACGAFVGLSAPGEAGSWQRECKG 204
           WPYSFPASEDFPKKDQRGIVSGRLLVFDRYVDQDYLPACGAFVGLSAPGEAGSWQRECKG
Sbjct: 316 WPYSFPASEDFPKKDQRGIVSGRLLVFDRYVDQDYLPACGAFVGLSAPGEAGSWQRECKG 375

Query: 205 YQFWTRADDGGYFTINHVHTGEYNLYAWVPGFIGDYRCDALINVSQGSCIEMGDLVYEPP 264
           YQFWTRADDGGYFTINHVHTGEYNLYAWVPGFIGDYRCDALINVSQGSCIEMGDLVYEPP
Sbjct: 376 YQFWTRADDGGYFTINHVHTGEYNLYAWVPGFIGDYRCDALINVSQGSCIEMGDLVYEPP 435

Query: 265 RDGPTLWEIGIPDRSASEFYVPDPNPIHINKLFVDHPDRFRQYGLWNRYAELYPNEDLVY 324
           RDGPTLWEIGIPDRSASEFYVPDPNPIHINKLFVDHPDRFRQYGLWNRYAELYPNEDLVY
Sbjct: 436 RDGPTLWEIGIPDRSASEFYVPDPNPIHINKLFVDHPDRFRQYGLWNRYAELYPNEDLVY 495

Query: 325 TVGTSDYRKDWFFAHVPRKTNNSHEGTTWQIKFKLDRVDHKSMYTLRVAIASATLAELQV 384
           TVGTSDYRKDWFFAHVPRKTNNSHEGTTWQIKFKLDRVDHKSMYTLRVAIASATLAELQV
Sbjct: 496 TVGTSDYRKDWFFAHVPRKTNNSHEGTTWQIKFKLDRVDHKSMYTLRVAIASATLAELQV 555

Query: 385 RVNDPRSKRPLFTSGLIGRDNSIARHGIHGIYWVYGVGVAGCHLVEGENTIFLTQPRCTS 444
           RVNDPRSKRPLFTSGLIGRDNSIARHGIHGIYWVYGVGVAGCHLVEGENTIFLTQPRCTS
Sbjct: 556 RVNDPRSKRPLFTSGLIGRDNSIARHGIHGIYWVYGVGVAGCHLVEGENTIFLTQPRCTS 615

Query: 445 PFQGIMYDYIRLEGPPTK 462
           PFQGIMYDYIRLEGPPTK
Sbjct: 616 PFQGIMYDYIRLEGPPTK 633

[Close]

BLAST of Cucsat.G12992 vs. ExPASy TrEMBL
Match: A0A5D3E6J1 (Rhamnogalacturonan endolyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G005450 PE=3 SV=1)

HSP 1 Score: 898 bits (2321), Expect = 0.0
Identity = 416/439 (94.76%), Postives = 425/439 (96.81%), Query Frame = 0


Query: 25  LLSRVDDKYQYSCENNNLKVHGWISTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTL 84
           L   VDDKYQYSCENNNLKVHGWISTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTL
Sbjct: 196 LKGEVDDKYQYSCENNNLKVHGWISTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTL 255

Query: 85  AIFLSTHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSVGDDPFWLWEDAKIQMMAEVDS 144
           AIFLS HYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSVGDDPFWLWEDAKIQMMAEV+S
Sbjct: 256 AIFLSAHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSVGDDPFWLWEDAKIQMMAEVES 315

Query: 145 WPYSFPASEDFPKKDQRGIVSGRLLVFDRYVDQDYLPACGAFVGLSAPGEAGSWQRECKG 204
           WPYSFPASEDF KKDQRG+VSGRLLVFDRYVDQDYLP  GAFVGLSAPGE GSWQRECKG
Sbjct: 316 WPYSFPASEDFSKKDQRGMVSGRLLVFDRYVDQDYLPGSGAFVGLSAPGEVGSWQRECKG 375

Query: 205 YQFWTRADDGGYFTINHVHTGEYNLYAWVPGFIGDYRCDALINVSQGSCIEMGDLVYEPP 264
           YQFWTRADDGGYFTINHV TG+YNLYAWVPGFIGDYRCDAL+NVSQGSCIEMGDLVYEPP
Sbjct: 376 YQFWTRADDGGYFTINHVLTGDYNLYAWVPGFIGDYRCDALVNVSQGSCIEMGDLVYEPP 435

Query: 265 RDGPTLWEIGIPDRSASEFYVPDPNPIHINKLFVDHPDRFRQYGLWNRYAELYPNEDLVY 324
           RDGPTLWEIGIPDRSASEFYVPDPNPI+INKLFV+HPDRFRQYGLW+RYAELYP+EDLVY
Sbjct: 436 RDGPTLWEIGIPDRSASEFYVPDPNPIYINKLFVNHPDRFRQYGLWDRYAELYPDEDLVY 495

Query: 325 TVGTSDYRKDWFFAHVPRKTN-NSHEGTTWQIKFKLDRVDHKSMYTLRVAIASATLAELQ 384
           TVGTSDYRKDWFFAHVPRKTN NSHEGTTWQIKF+LDRVD KSMY LRVAIASATLAELQ
Sbjct: 496 TVGTSDYRKDWFFAHVPRKTNSNSHEGTTWQIKFQLDRVDRKSMYMLRVAIASATLAELQ 555

Query: 385 VRVNDPRSKRPLFTSGLIGRDNSIARHGIHGIYWVYGVGVAGCHLVEGENTIFLTQPRCT 444
           VRVNDPRSKRPLFTSGLIGRDNSIARHGIHGIYWVY V VAGCHLVEGENTIFLTQPRCT
Sbjct: 556 VRVNDPRSKRPLFTSGLIGRDNSIARHGIHGIYWVYSVSVAGCHLVEGENTIFLTQPRCT 615

Query: 445 SPFQGIMYDYIRLEGPPTK 462
           SPFQGIMYDYIRLEGPPTK
Sbjct: 616 SPFQGIMYDYIRLEGPPTK 634

[Close]

BLAST of Cucsat.G12992 vs. ExPASy TrEMBL
Match: A0A1S3CQJ4 (Rhamnogalacturonan endolyase OS=Cucumis melo OX=3656 GN=LOC103503644 PE=3 SV=1)

HSP 1 Score: 898 bits (2321), Expect = 0.0
Identity = 416/439 (94.76%), Postives = 425/439 (96.81%), Query Frame = 0


Query: 25  LLSRVDDKYQYSCENNNLKVHGWISTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTL 84
           L   VDDKYQYSCENNNLKVHGWISTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTL
Sbjct: 196 LKGEVDDKYQYSCENNNLKVHGWISTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTL 255

Query: 85  AIFLSTHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSVGDDPFWLWEDAKIQMMAEVDS 144
           AIFLS HYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSVGDDPFWLWEDAKIQMMAEV+S
Sbjct: 256 AIFLSAHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSVGDDPFWLWEDAKIQMMAEVES 315

Query: 145 WPYSFPASEDFPKKDQRGIVSGRLLVFDRYVDQDYLPACGAFVGLSAPGEAGSWQRECKG 204
           WPYSFPASEDF KKDQRG+VSGRLLVFDRYVDQDYLP  GAFVGLSAPGE GSWQRECKG
Sbjct: 316 WPYSFPASEDFSKKDQRGMVSGRLLVFDRYVDQDYLPGSGAFVGLSAPGEVGSWQRECKG 375

Query: 205 YQFWTRADDGGYFTINHVHTGEYNLYAWVPGFIGDYRCDALINVSQGSCIEMGDLVYEPP 264
           YQFWTRADDGGYFTINHV TG+YNLYAWVPGFIGDYRCDAL+NVSQGSCIEMGDLVYEPP
Sbjct: 376 YQFWTRADDGGYFTINHVLTGDYNLYAWVPGFIGDYRCDALVNVSQGSCIEMGDLVYEPP 435

Query: 265 RDGPTLWEIGIPDRSASEFYVPDPNPIHINKLFVDHPDRFRQYGLWNRYAELYPNEDLVY 324
           RDGPTLWEIGIPDRSASEFYVPDPNPI+INKLFV+HPDRFRQYGLW+RYAELYP+EDLVY
Sbjct: 436 RDGPTLWEIGIPDRSASEFYVPDPNPIYINKLFVNHPDRFRQYGLWDRYAELYPDEDLVY 495

Query: 325 TVGTSDYRKDWFFAHVPRKTN-NSHEGTTWQIKFKLDRVDHKSMYTLRVAIASATLAELQ 384
           TVGTSDYRKDWFFAHVPRKTN NSHEGTTWQIKF+LDRVD KSMY LRVAIASATLAELQ
Sbjct: 496 TVGTSDYRKDWFFAHVPRKTNSNSHEGTTWQIKFQLDRVDRKSMYMLRVAIASATLAELQ 555

Query: 385 VRVNDPRSKRPLFTSGLIGRDNSIARHGIHGIYWVYGVGVAGCHLVEGENTIFLTQPRCT 444
           VRVNDPRSKRPLFTSGLIGRDNSIARHGIHGIYWVY V VAGCHLVEGENTIFLTQPRCT
Sbjct: 556 VRVNDPRSKRPLFTSGLIGRDNSIARHGIHGIYWVYSVSVAGCHLVEGENTIFLTQPRCT 615

Query: 445 SPFQGIMYDYIRLEGPPTK 462
           SPFQGIMYDYIRLEGPPTK
Sbjct: 616 SPFQGIMYDYIRLEGPPTK 634

[Close]

BLAST of Cucsat.G12992 vs. ExPASy TrEMBL
Match: A0A1S3CQI1 (Rhamnogalacturonan endolyase OS=Cucumis melo OX=3656 GN=LOC103503644 PE=3 SV=1)

HSP 1 Score: 898 bits (2321), Expect = 0.0
Identity = 416/439 (94.76%), Postives = 425/439 (96.81%), Query Frame = 0


Query: 25  LLSRVDDKYQYSCENNNLKVHGWISTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTL 84
           L   VDDKYQYSCENNNLKVHGWISTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTL
Sbjct: 102 LKGEVDDKYQYSCENNNLKVHGWISTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTL 161

Query: 85  AIFLSTHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSVGDDPFWLWEDAKIQMMAEVDS 144
           AIFLS HYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSVGDDPFWLWEDAKIQMMAEV+S
Sbjct: 162 AIFLSAHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSVGDDPFWLWEDAKIQMMAEVES 221

Query: 145 WPYSFPASEDFPKKDQRGIVSGRLLVFDRYVDQDYLPACGAFVGLSAPGEAGSWQRECKG 204
           WPYSFPASEDF KKDQRG+VSGRLLVFDRYVDQDYLP  GAFVGLSAPGE GSWQRECKG
Sbjct: 222 WPYSFPASEDFSKKDQRGMVSGRLLVFDRYVDQDYLPGSGAFVGLSAPGEVGSWQRECKG 281

Query: 205 YQFWTRADDGGYFTINHVHTGEYNLYAWVPGFIGDYRCDALINVSQGSCIEMGDLVYEPP 264
           YQFWTRADDGGYFTINHV TG+YNLYAWVPGFIGDYRCDAL+NVSQGSCIEMGDLVYEPP
Sbjct: 282 YQFWTRADDGGYFTINHVLTGDYNLYAWVPGFIGDYRCDALVNVSQGSCIEMGDLVYEPP 341

Query: 265 RDGPTLWEIGIPDRSASEFYVPDPNPIHINKLFVDHPDRFRQYGLWNRYAELYPNEDLVY 324
           RDGPTLWEIGIPDRSASEFYVPDPNPI+INKLFV+HPDRFRQYGLW+RYAELYP+EDLVY
Sbjct: 342 RDGPTLWEIGIPDRSASEFYVPDPNPIYINKLFVNHPDRFRQYGLWDRYAELYPDEDLVY 401

Query: 325 TVGTSDYRKDWFFAHVPRKTN-NSHEGTTWQIKFKLDRVDHKSMYTLRVAIASATLAELQ 384
           TVGTSDYRKDWFFAHVPRKTN NSHEGTTWQIKF+LDRVD KSMY LRVAIASATLAELQ
Sbjct: 402 TVGTSDYRKDWFFAHVPRKTNSNSHEGTTWQIKFQLDRVDRKSMYMLRVAIASATLAELQ 461

Query: 385 VRVNDPRSKRPLFTSGLIGRDNSIARHGIHGIYWVYGVGVAGCHLVEGENTIFLTQPRCT 444
           VRVNDPRSKRPLFTSGLIGRDNSIARHGIHGIYWVY V VAGCHLVEGENTIFLTQPRCT
Sbjct: 462 VRVNDPRSKRPLFTSGLIGRDNSIARHGIHGIYWVYSVSVAGCHLVEGENTIFLTQPRCT 521

Query: 445 SPFQGIMYDYIRLEGPPTK 462
           SPFQGIMYDYIRLEGPPTK
Sbjct: 522 SPFQGIMYDYIRLEGPPTK 540

[Close]

BLAST of Cucsat.G12992 vs. ExPASy TrEMBL
Match: A0A6J1FQM1 (Rhamnogalacturonan endolyase OS=Cucurbita moschata OX=3662 GN=LOC111446358 PE=3 SV=1)

HSP 1 Score: 838 bits (2164), Expect = 1.73e-302
Identity = 383/435 (88.05%), Postives = 403/435 (92.64%), Query Frame = 0


Query: 29  VDDKYQYSCENNNLKVHGWISTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTLAIFL 88
           VDDKYQYSC+N +LKVHGWISTNPP GFWQITPSDEFRSGGP KQSLTSHVGPTTLAIFL
Sbjct: 200 VDDKYQYSCQNKDLKVHGWISTNPPIGFWQITPSDEFRSGGPHKQSLTSHVGPTTLAIFL 259

Query: 89  STHYAGQDLVPKFRAGEPWKKVFGPVFLYLNSTSVGDDPFWLWEDAKIQMMAEVDSWPYS 148
           STHYAGQDLVPKFR GEPWKKVFGPVFLYLNST  GDDPFWLWEDAKIQMMAEV SWPYS
Sbjct: 260 STHYAGQDLVPKFRNGEPWKKVFGPVFLYLNSTKAGDDPFWLWEDAKIQMMAEVQSWPYS 319

Query: 149 FPASEDFPKKDQRGIVSGRLLVFDRYVDQDYLPACGAFVGLSAPGEAGSWQRECKGYQFW 208
           FPAS+DFPK DQRG +SGRLLVFDRY+ +DYLPA GAFVGLSAPGE GSWQRECKGYQFW
Sbjct: 320 FPASDDFPKLDQRGTISGRLLVFDRYMSRDYLPAGGAFVGLSAPGEVGSWQRECKGYQFW 379

Query: 209 TRADDGGYFTINHVHTGEYNLYAWVPGFIGDYRCDALINVSQGSCIEMGDLVYEPPRDGP 268
           TRADD GYF INHVH G+YNLYAWVPGFIGDYRCDALIN+SQGS IE+GDLVYEPPRDGP
Sbjct: 380 TRADDRGYFIINHVHIGQYNLYAWVPGFIGDYRCDALINISQGSSIELGDLVYEPPRDGP 439

Query: 269 TLWEIGIPDRSASEFYVPDPNPIHINKLFVDHPDRFRQYGLWNRYAELYPNEDLVYTVGT 328
           TLWEIGIPDRSASEFYVPDPNP+HINKLFV+HP+RFRQYGLW RYAELYP+EDLVYTVGT
Sbjct: 440 TLWEIGIPDRSASEFYVPDPNPLHINKLFVNHPERFRQYGLWERYAELYPDEDLVYTVGT 499

Query: 329 SDYRKDWFFAHVPRKTN-NSHEGTTWQIKFKLDRVDHKSMYTLRVAIASATLAELQVRVN 388
           SDY KDWFFA VPRKTN NSH+GTTW+I FKLDRVD K+ Y LRVAIASATLAELQ+RVN
Sbjct: 500 SDYSKDWFFAQVPRKTNDNSHQGTTWKIAFKLDRVDRKNRYMLRVAIASATLAELQIRVN 559

Query: 389 DPRSKRPLFTSGLIGRDNSIARHGIHGIYWVYGVGVAGCHLVEGENTIFLTQPRCTSPFQ 448
           DP SKRPLFTSGLIGRDNSIARHGIHGIYW+Y VG+AG HLVEG+N IF TQPRCTSPFQ
Sbjct: 560 DPSSKRPLFTSGLIGRDNSIARHGIHGIYWLYSVGIAGLHLVEGDNAIFFTQPRCTSPFQ 619

Query: 449 GIMYDYIRLEGPPTK 462
           GIMYDYIRLE P T 
Sbjct: 620 GIMYDYIRLEAPSTN 634

The following BLAST results are available for this feature:

BLAST of Cucsat.G12992 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A5ABH4	8.3e-18	25.73	Probable rhamnogalacturonate lyase B OS=Aspergillus niger (strain CBS 513.88 / F...	[more]
Q5AZ85	1.2e-13	24.47	Rhamnogalacturonate lyase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 ...	[more]

BLAST of Cucsat.G12992 vs. NCBI nr


Match Name	Identity	Description
XP_031739419.1	99.32	probable rhamnogalacturonate lyase B isoform X2 [Cucumis sativus]	[more]
XP_004136250.1	99.32	probable rhamnogalacturonate lyase B isoform X1 [Cucumis sativus] >KGN60276.1 hy...	[more]
XP_008466137.1	94.76	PREDICTED: probable rhamnogalacturonate lyase B isoform X1 [Cucumis melo] >KAA00...	[more]
XP_008466138.1	94.76	PREDICTED: probable rhamnogalacturonate lyase B isoform X2 [Cucumis melo]	[more]
XP_038897922.1	91.57	probable rhamnogalacturonate lyase B [Benincasa hispida]	[more]

BLAST of Cucsat.G12992 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LJQ9	0.0	99.32	Rhamnogalacturonan endolyase OS=Cucumis sativus OX=3659 GN=Csa_3G892770 PE=3 SV=...	[more]
A0A5D3E6J1	0.0	94.76	Rhamnogalacturonan endolyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A1S3CQJ4	0.0	94.76	Rhamnogalacturonan endolyase OS=Cucumis melo OX=3656 GN=LOC103503644 PE=3 SV=1	[more]
A0A1S3CQI1	0.0	94.76	Rhamnogalacturonan endolyase OS=Cucumis melo OX=3656 GN=LOC103503644 PE=3 SV=1	[more]
A0A6J1FQM1	1.73e-302	88.05	Rhamnogalacturonan endolyase OS=Cucurbita moschata OX=3662 GN=LOC111446358 PE=3 ...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029411	Rhamnogalacturonan lyase, domain III	PFAM	PF14683	CBM-like	coord: 14..201 e-value: 1.1E-56 score: 191.3
None	No IPR available	GENE3D	2.60.120.260		coord: 12..202 e-value: 4.2E-22 score: 80.5
None	No IPR available	PANTHER	PTHR32018	RHAMNOGALACTURONATE LYASE FAMILY PROTEIN	coord: 1..206
None	No IPR available	PANTHER	PTHR32018:SF8	RHAMNOGALACTURONATE LYASE FAMILY PROTEIN	coord: 1..206
None	No IPR available	CDD	cd10317	RGL4_C	coord: 16..202 e-value: 4.77226E-59 score: 180.934
IPR008979	Galactose-binding-like domain superfamily	SUPERFAMILY	49785	Galactose-binding domain-like	coord: 11..202

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G12992.T2	Cucsat.G12992.T2	mRNA
Cucsat.G12992.T1	Cucsat.G12992.T1	mRNA
Cucsat.G12992.T13	Cucsat.G12992.T13	mRNA
Cucsat.G12992.T9	Cucsat.G12992.T9	mRNA
Cucsat.G12992.T3	Cucsat.G12992.T3	mRNA
Cucsat.G12992.T7	Cucsat.G12992.T7	mRNA
Cucsat.G12992.T6	Cucsat.G12992.T6	mRNA
Cucsat.G12992.T5	Cucsat.G12992.T5	mRNA
Cucsat.G12992.T4	Cucsat.G12992.T4	mRNA
Cucsat.G12992.T12	Cucsat.G12992.T12	mRNA
Cucsat.G12992.T11	Cucsat.G12992.T11	mRNA
Cucsat.G12992.T10	Cucsat.G12992.T10	mRNA
Cucsat.G12992.T15	Cucsat.G12992.T15	mRNA
Cucsat.G12992.T8	Cucsat.G12992.T8	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0030246	carbohydrate binding
molecular_function	GO:0102210	rhamnogalacturonan endolyase activity
molecular_function	GO:0003824	catalytic activity