Cucsat.G12338 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G12338
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Pectin lyase-like superfamily protein
Location	ctg1837: 457407 .. 459158 (+)
RNA-Seq Expression	Cucsat.G12338
Synteny	Cucsat.G12338

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TCGACTATTTTCACAGGACCGTGCAAAAATGGCGTTGTGTTTCAAGTTGATGGCACTCTTATGGCACCTGACGGGCCTGACGCTTGGCCGAAGGGTTATAGTGCTCACCAATGGTTGGTTTTTTATCGAGTTTATAACATGTCACTTCAAGGAAATGGCCTTATTGATGGTCGAGGCCAAAAATGGTGGGATCTTCCTTGCAAACCTCACAAGGTAAGGGACAACAACGATCATAGGTCTAGAAATCTTTTTACTTCATTTCGAGGTCGTTTTGAAAGCTAACTTGAGGTTTTGATTCTATTTTTAAAGGGGAAAACAATGACAGGACCTTGTGACAGCCCAATTGTAAGTTAAAATTCAGTTCTCTATAATAAAACTTTTTTTTTTTCTTTTAAAGTTTGCAAATATAATGAAAAAGTCAAGTGAAAAGAGTTAAAAGTGATTTTTTTTGACAGGCTATAAGATTTTTCATGAGCTCAGAGTTGAGAGTTGAAGGAGTTAGAATCAAAGACAGTCCTCAATTCCATTTTAGGTTTGACAACTGTAAAGATGTTTATATTGATTCTCTTCACATAACATCTCCTGCTTTGAGCCCAAACACTGATGGAATCCACATTGAGAATACAAATGGTGTTCAGATTTTCAATTCAATAGTTGCCAATGGTGAGTCCTCATACACATATAACATTGTTTCCTAATTATCTTCTCTTTGCATTTTAATTTCTAAAAACGACAGAATAATTTTTCAAAGTAAAAACCACAGTAGAAATGAGTAGGTGTATCACTATTGGATAATGTTCTCCTTTCTAGTTTTTTATTACAAAATTGTGGTTTTGTTTTGAAAAAATATATCTAAATACATTTTTTAGACTAAAACAATAAACTATTTCTTTTTCTTTTAAAAAATTGTGTAAACATTTGGTGTGTTTCAAAGCTAAAGAATTAGAGACATCACAATAAATATATATGTTAAACTCATGACATTTCTTAAGAAATTAGAAACAAGAAAAAAGAATAAAATGTTAATTATAATATATATTCTTGTATTTTGATTTCTCGTTTATCATTTTCTATTTTTGTTGCTTGTTTTTACTTTTAATAGTGAAAAACATAATAATTGTATTTGGTAACTATTTCTTCAATATCTTATTTATTAAAAATATATTTTAAAAAACGTTGAACATACGAACACTTTTAAGTTGTTTTTATTTTTAAATATTTATTAGTGATTTTACAATCACAGATGGTTAAAGATTATTTCATAATATTTTATGTCGGAATAACGTTGTACTTAAATAAAGAACAAAATTAAATATGTTTTTAAGTAGTTGAAAGGAGGAAACAAAAAATGACAAGCGTAAACGTTACCAAATAATTAATTTCCTCAGATTTAAAATAGAACTTATGAAGCAACGACTCCTACCAAATTTTTTTCACGCAGGTGATGATTGTGTTTCAATTGGATCGGGTTCATTTAACGTAGAAATAAGAAACATTACATGTGGACCAGGTCATGGAATTAGGTAAACAAATCATGAAATCTTATTAAAATTTCTTCTATAATTAATTCTTATATAACATGTAATTTCTTTTTATACTCACAACCCTAGTTCTTCATGTCAGCATTGGTAGTCTAGGGAATCACAACTCACGAGCGTGCGTATCGAACATAACAGTTCGGGACTCAATAATTAAGGTCTCAGACAATGGTGTTCGAATCAAGACATGGCAAGGTGGATTTGGTACCGTAAG

Coding sequence (CDS)

TCGACTATTTTCACAGGACCGTGCAAAAATGGCGTTGTGTTTCAAGTTGATGGCACTCTTATGGCACCTGACGGGCCTGACGCTTGGCCGAAGGGTTATAGTGCTCACCAATGGTTGGTTTTTTATCGAGTTTATAACATGTCACTTCAAGGAAATGGCCTTATTGATGGTCGAGGCCAAAAATGGTGGGATCTTCCTTGCAAACCTCACAAGGGGAAAACAATGACAGGACCTTGTGACAGCCCAATTGCTATAAGATTTTTCATGAGCTCAGAGTTGAGAGTTGAAGGAGTTAGAATCAAAGACAGTCCTCAATTCCATTTTAGGTTTGACAACTGTAAAGATGTTTATATTGATTCTCTTCACATAACATCTCCTGCTTTGAGCCCAAACACTGATGGAATCCACATTGAGAATACAAATGGTGTTCAGATTTTCAATTCAATAGTTGCCAATGGTGATGATTGTGTTTCAATTGGATCGGGTTCATTTAACGTAGAAATAAGAAACATTACATGTGGACCAGGTCATGGAATTAGCATTGGTAGTCTAGGGAATCACAACTCACGAGCGTGCGTATCGAACATAACAGTTCGGGACTCAATAATTAAGGTCTCAGACAATGGTGTTCGAATCAAGACATGGCAAGGTGGATTTGGTACCGTA

Protein sequence

STIFTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNGLIDGRGQKWWDLPCKPHKGKTMTGPCDSPIAIRFFMSSELRVEGVRIKDSPQFHFRFDNCKDVYIDSLHITSPALSPNTDGIHIENTNGVQIFNSIVANGDDCVSIGSGSFNVEIRNITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGTV

Homology

[Close]

BLAST of Cucsat.G12338 vs. ExPASy Swiss-Prot
Match: Q949Z1 (Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV=1)

HSP 1 Score: 323.2 bits (827), Expect = 2.4e-87
Identity = 139/225 (61.78%), Postives = 182/225 (80.89%), Query Frame = 0


Query: 1   STIFTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNGLIDGRGQ 60
           STIF+GPCK G+VFQ+DG LM PDGP+ WP+  + +QWLVFYR+   +  G G ++G GQ
Sbjct: 126 STIFSGPCKPGLVFQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTVEGNGQ 185

Query: 61  KWWDLPCKPHK---GKTMTGPCDSPIAIRFFMSSELRVEGVRIKDSPQFHFRFDNCKDVY 120
           KWWDLPCKPH+   G + +GPC SP  IRFFMS+ + V+G+RI++SPQFH +FD C+ V 
Sbjct: 186 KWWDLPCKPHRGPDGSSSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDGCQGVL 245

Query: 121 IDSLHITSPALSPNTDGIHIENTNGVQIFNSIVANGDDCVSIGSGSFNVEIRNITCGPGH 180
           I+ + I+SP LSPNTDGIH+ NT  V I+NS+V+NGDDC+SIG+G  +V+I+ +TCGP H
Sbjct: 246 INEIQISSPKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQGVTCGPSH 305

Query: 181 GISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGTV 223
           GISIGSLG HNS+ACVSNITVR+++I+ SDNG+R+KTWQGG G+V
Sbjct: 306 GISIGSLGVHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGSV 350

[Close]

BLAST of Cucsat.G12338 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 209.1 bits (531), Expect = 5.0e-53
Identity = 99/219 (45.21%), Postives = 138/219 (63.01%), Query Frame = 0


Query: 5   TGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNGLIDGRGQKWWD 64
           +GPCK  +  Q+ GT++AP+ PD W +G +  +WL F+ +  ++++G G ++G GQ+WW+
Sbjct: 104 SGPCKARLTLQISGTIIAPNDPDVW-EGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWE 163

Query: 65  LPCKPHKGKTMTGPC-DSPIAIRFFMSSELRVEGVRIKDSPQFHFRFDNCKDVYIDSLHI 124
             CK +     + PC  +P A+ F     +RVE + + DS Q H    +C+ V I  L +
Sbjct: 164 RSCKHNH----SNPCRGAPTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKV 223

Query: 125 TSPALSPNTDGIHIENTNGVQIFNSIVANGDDCVSIGSGSFNVEIRNITCGPGHGISIGS 184
            +PA SPNTDGIHI  + G+ I N+ V+ GDDC+SI   S  + I NI CGPGHGISIGS
Sbjct: 224 IAPATSPNTDGIHISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGS 283

Query: 185 LGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGTV 223
           LG   S   V +ITV  +II  + NGVRIKTWQGG G V
Sbjct: 284 LGKSKSWEEVRDITVDTAIISDTANGVRIKTWQGGSGLV 317

[Close]

BLAST of Cucsat.G12338 vs. ExPASy Swiss-Prot
Match: P35336 (Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 6.5e-53
Identity = 99/219 (45.21%), Postives = 140/219 (63.93%), Query Frame = 0


Query: 4   FTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNGLIDGRGQKWW 63
           F+GPCK+G+  Q+ GT+ A D    + K      WLVF  V N+ ++G G I+G G+ WW
Sbjct: 138 FSGPCKSGLTMQIYGTIEASDDRSDYRK--DGRHWLVFDSVQNLRVEGGGTINGNGKIWW 197

Query: 64  DLPCKPHKGKTMTGPC-DSPIAIRFFMSSELRVEGVRIKDSPQFHFRFDNCKDVYIDSLH 123
              CK +K      PC D+P A+ F+ S  + V+ ++I+++ Q H  FDNC +V   +L 
Sbjct: 198 QNSCKTNKAL----PCKDAPTALTFYKSKHVIVKNLKIENAQQIHVSFDNCVNVQASNLM 257

Query: 124 ITSPALSPNTDGIHIENTNGVQIFNSIVANGDDCVSIGSGSFNVEIRNITCGPGHGISIG 183
           +T+P  SPNTDGIH+  T  + I + ++  GDDC+SI +GS  V + +ITCGPGHGISIG
Sbjct: 258 VTAPENSPNTDGIHVTGTQNIHISSCVIGTGDDCISIVNGSRKVRVNDITCGPGHGISIG 317

Query: 184 SLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGT 222
           SLG  NS A VS++ V  + +  + NGVRIKTWQGG G+
Sbjct: 318 SLGYGNSEAHVSDVVVNGAKLCGTTNGVRIKTWQGGSGS 350

[Close]

BLAST of Cucsat.G12338 vs. ExPASy Swiss-Prot
Match: Q02096 (Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 9.4e-52
Identity = 100/219 (45.66%), Postives = 134/219 (61.19%), Query Frame = 0


Query: 4   FTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNGLIDGRGQKWW 63
           F+GPCK+ +  ++ GT+ A      W  G++  +W+ F  + N++L+G G  +G G+ WW
Sbjct: 132 FSGPCKSDLRVKIRGTIEASSDQSDW-VGHNRKRWIEFEDISNLTLEGGGTSNGNGETWW 191

Query: 64  DLPCKPHKGKTMTGPCDS-PIAIRFFMSSELRVEGVRIKDSPQFHFRFDNCKDVYIDSLH 123
           D  CK  K      PC S P A+ F     L V  + IKDS + H  FD C+DV   +L 
Sbjct: 192 DSSCKRKKSL----PCKSAPTALTFRSCKNLIVSDLSIKDSQKMHLSFDKCQDVIASNLM 251

Query: 124 ITSPALSPNTDGIHIENTNGVQIFNSIVANGDDCVSIGSGSFNVEIRNITCGPGHGISIG 183
           +T+P  SPNTDGIHI  T  + + NS++  GDDC+SI SGS  V   NITCGPGHGISIG
Sbjct: 252 VTAPEHSPNTDGIHITGTQRIHVMNSVIGTGDDCISIESGSKMVIATNITCGPGHGISIG 311

Query: 184 SLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGT 222
           SLG+ NS A VS + V    +  + NG+RIKTWQGG G+
Sbjct: 312 SLGDRNSEAHVSGVLVDGGNLFDTTNGLRIKTWQGGSGS 345

[Close]

BLAST of Cucsat.G12338 vs. ExPASy Swiss-Prot
Match: P05117 (Polygalacturonase-2 OS=Solanum lycopersicum OX=4081 GN=PG2 PE=1 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 1.2e-51
Identity = 99/218 (45.41%), Postives = 140/218 (64.22%), Query Frame = 0


Query: 4   FTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNGLIDGRGQKWW 63
           F+GPC++ +  ++ G+L A      +        W+ F  V N+ + G G I+G GQ WW
Sbjct: 126 FSGPCRSSISVKIFGSLEASSKISDYK---DRRLWIAFDSVQNLVVGGGGTINGNGQVWW 185

Query: 64  DLPCKPHKGKTMTGPC-DSPIAIRFFMSSELRVEGVRIKDSPQFHFRFDNCKDVYIDSLH 123
              CK +K      PC D+P A+ F+    L+V  ++ K++ Q H +F++C +V   +L 
Sbjct: 186 PSSCKINKSL----PCRDAPTALTFWNCKNLKVNNLKSKNAQQIHIKFESCTNVVASNLM 245

Query: 124 ITSPALSPNTDGIHIENTNGVQIFNSIVANGDDCVSIGSGSFNVEIRNITCGPGHGISIG 183
           I + A SPNTDG+H+ NT  +QI ++I+  GDDC+SI SGS NV+  NITCGPGHGISIG
Sbjct: 246 INASAKSPNTDGVHVSNTQYIQISDTIIGTGDDCISIVSGSQNVQATNITCGPGHGISIG 305

Query: 184 SLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFG 221
           SLG+ NS A VSN+TV ++ I  ++NGVRIKTWQGG G
Sbjct: 306 SLGSGNSEAYVSNVTVNEAKIIGAENGVRIKTWQGGSG 336

[Close]

BLAST of Cucsat.G12338 vs. NCBI nr
Match: XP_031737994.1 (polygalacturonase At1g48100 [Cucumis sativus] >KGN58717.2 hypothetical protein Csa_000831 [Cucumis sativus])

HSP 1 Score: 475 bits (1222), Expect = 1.93e-165
Identity = 222/222 (100.00%), Postives = 222/222 (100.00%), Query Frame = 0


Query: 1   STIFTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNGLIDGRGQ 60
           STIFTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNGLIDGRGQ
Sbjct: 138 STIFTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNGLIDGRGQ 197

Query: 61  KWWDLPCKPHKGKTMTGPCDSPIAIRFFMSSELRVEGVRIKDSPQFHFRFDNCKDVYIDS 120
           KWWDLPCKPHKGKTMTGPCDSPIAIRFFMSSELRVEGVRIKDSPQFHFRFDNCKDVYIDS
Sbjct: 198 KWWDLPCKPHKGKTMTGPCDSPIAIRFFMSSELRVEGVRIKDSPQFHFRFDNCKDVYIDS 257

Query: 121 LHITSPALSPNTDGIHIENTNGVQIFNSIVANGDDCVSIGSGSFNVEIRNITCGPGHGIS 180
           LHITSPALSPNTDGIHIENTNGVQIFNSIVANGDDCVSIGSGSFNVEIRNITCGPGHGIS
Sbjct: 258 LHITSPALSPNTDGIHIENTNGVQIFNSIVANGDDCVSIGSGSFNVEIRNITCGPGHGIS 317

Query: 181 IGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGTV 222
           IGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGTV
Sbjct: 318 IGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGTV 359

[Close]

BLAST of Cucsat.G12338 vs. NCBI nr
Match: XP_016900401.1 (PREDICTED: polygalacturonase At1g48100, partial [Cucumis melo])

HSP 1 Score: 465 bits (1196), Expect = 7.39e-162
Identity = 214/222 (96.40%), Postives = 220/222 (99.10%), Query Frame = 0


Query: 1   STIFTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNGLIDGRGQ 60
           STIFTGPC+NG+VFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNGLIDGRGQ
Sbjct: 113 STIFTGPCRNGIVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNGLIDGRGQ 172

Query: 61  KWWDLPCKPHKGKTMTGPCDSPIAIRFFMSSELRVEGVRIKDSPQFHFRFDNCKDVYIDS 120
           KWWDLPCKPHKGKTMTGPCDSPIAIRFFMSS+LRVEGVRIKDSPQFHFRFDNCKDVYIDS
Sbjct: 173 KWWDLPCKPHKGKTMTGPCDSPIAIRFFMSSKLRVEGVRIKDSPQFHFRFDNCKDVYIDS 232

Query: 121 LHITSPALSPNTDGIHIENTNGVQIFNSIVANGDDCVSIGSGSFNVEIRNITCGPGHGIS 180
           LHITSPALSPNTDGIHIENTNGVQIFNSIVANGDDC+SIGSGS+NVEI NITCGPGHGIS
Sbjct: 233 LHITSPALSPNTDGIHIENTNGVQIFNSIVANGDDCISIGSGSYNVEITNITCGPGHGIS 292

Query: 181 IGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGTV 222
           IGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGG+G V
Sbjct: 293 IGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGYGAV 334

[Close]

BLAST of Cucsat.G12338 vs. NCBI nr
Match: TYK20501.1 (polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 465 bits (1196), Expect = 2.33e-161
Identity = 214/222 (96.40%), Postives = 220/222 (99.10%), Query Frame = 0


Query: 1   STIFTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNGLIDGRGQ 60
           STIFTGPC+NG+VFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNGLIDGRGQ
Sbjct: 147 STIFTGPCRNGIVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNGLIDGRGQ 206

Query: 61  KWWDLPCKPHKGKTMTGPCDSPIAIRFFMSSELRVEGVRIKDSPQFHFRFDNCKDVYIDS 120
           KWWDLPCKPHKGKTMTGPCDSPIAIRFFMSS+LRVEGVRIKDSPQFHFRFDNCKDVYIDS
Sbjct: 207 KWWDLPCKPHKGKTMTGPCDSPIAIRFFMSSKLRVEGVRIKDSPQFHFRFDNCKDVYIDS 266

Query: 121 LHITSPALSPNTDGIHIENTNGVQIFNSIVANGDDCVSIGSGSFNVEIRNITCGPGHGIS 180
           LHITSPALSPNTDGIHIENTNGVQIFNSIVANGDDC+SIGSGS+NVEI NITCGPGHGIS
Sbjct: 267 LHITSPALSPNTDGIHIENTNGVQIFNSIVANGDDCISIGSGSYNVEITNITCGPGHGIS 326

Query: 181 IGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGTV 222
           IGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGG+G V
Sbjct: 327 IGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGYGAV 368

[Close]

BLAST of Cucsat.G12338 vs. NCBI nr
Match: XP_038905655.1 (polygalacturonase At1g48100-like [Benincasa hispida])

HSP 1 Score: 434 bits (1117), Expect = 1.40e-149
Identity = 202/225 (89.78%), Postives = 212/225 (94.22%), Query Frame = 0


Query: 1   STIFTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNGLIDGRGQ 60
           STIFTGPCKNGV FQV GTLMAPDGPDAWPK YSAHQWLVFYRVYNMSL+GNGLIDGRGQ
Sbjct: 129 STIFTGPCKNGVEFQVGGTLMAPDGPDAWPKSYSAHQWLVFYRVYNMSLRGNGLIDGRGQ 188

Query: 61  KWWDLPCKPHKG---KTMTGPCDSPIAIRFFMSSELRVEGVRIKDSPQFHFRFDNCKDVY 120
           KWWDLPCKPHKG   +TM+ PCDSPIAIRFFMSS L VEG+RIKDSPQFHFRFDNCKDV 
Sbjct: 189 KWWDLPCKPHKGVKGRTMSEPCDSPIAIRFFMSSNLTVEGLRIKDSPQFHFRFDNCKDVI 248

Query: 121 IDSLHITSPALSPNTDGIHIENTNGVQIFNSIVANGDDCVSIGSGSFNVEIRNITCGPGH 180
           ID +HIT+PALSPNTDGIHIENTNGVQIFNS VANGDDC+SIGSGS+NV+IRNITCGPGH
Sbjct: 249 IDGIHITAPALSPNTDGIHIENTNGVQIFNSEVANGDDCISIGSGSYNVDIRNITCGPGH 308

Query: 181 GISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGTV 222
           GISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGG+G V
Sbjct: 309 GISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGYGAV 353

[Close]

BLAST of Cucsat.G12338 vs. NCBI nr
Match: KAA0038107.1 (polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 432 bits (1112), Expect = 1.20e-148
Identity = 203/222 (91.44%), Postives = 212/222 (95.50%), Query Frame = 0


Query: 1   STIFTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNGLIDGRGQ 60
           STIFTGPC+NG+VFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNGLIDGRGQ
Sbjct: 147 STIFTGPCRNGIVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNGLIDGRGQ 206

Query: 61  KWWDLPCKPHKGKTMTGPCDSPIAIRFFMSSELRVEGVRIKDSPQFHFRFDNCKDVYIDS 120
           KWWDLPCKPHK K+++       AIRFFMSS+LRVEGVRIKDSPQFHFRFDNCKDVYIDS
Sbjct: 207 KWWDLPCKPHKSKSVSFFWQ---AIRFFMSSKLRVEGVRIKDSPQFHFRFDNCKDVYIDS 266

Query: 121 LHITSPALSPNTDGIHIENTNGVQIFNSIVANGDDCVSIGSGSFNVEIRNITCGPGHGIS 180
           LHITSPALSPNTDGIHIENTNGVQIFNSIVANGDDC+SIGSGS+NVEI NITCGPGHGIS
Sbjct: 267 LHITSPALSPNTDGIHIENTNGVQIFNSIVANGDDCISIGSGSYNVEITNITCGPGHGIS 326

Query: 181 IGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGTV 222
           IGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGG+G V
Sbjct: 327 IGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGYGAV 365

[Close]

BLAST of Cucsat.G12338 vs. ExPASy TrEMBL
Match: A0A0A0LEQ6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G664600 PE=3 SV=1)

HSP 1 Score: 475 bits (1222), Expect = 5.82e-166
Identity = 222/222 (100.00%), Postives = 222/222 (100.00%), Query Frame = 0


Query: 1   STIFTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNGLIDGRGQ 60
           STIFTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNGLIDGRGQ
Sbjct: 124 STIFTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNGLIDGRGQ 183

Query: 61  KWWDLPCKPHKGKTMTGPCDSPIAIRFFMSSELRVEGVRIKDSPQFHFRFDNCKDVYIDS 120
           KWWDLPCKPHKGKTMTGPCDSPIAIRFFMSSELRVEGVRIKDSPQFHFRFDNCKDVYIDS
Sbjct: 184 KWWDLPCKPHKGKTMTGPCDSPIAIRFFMSSELRVEGVRIKDSPQFHFRFDNCKDVYIDS 243

Query: 121 LHITSPALSPNTDGIHIENTNGVQIFNSIVANGDDCVSIGSGSFNVEIRNITCGPGHGIS 180
           LHITSPALSPNTDGIHIENTNGVQIFNSIVANGDDCVSIGSGSFNVEIRNITCGPGHGIS
Sbjct: 244 LHITSPALSPNTDGIHIENTNGVQIFNSIVANGDDCVSIGSGSFNVEIRNITCGPGHGIS 303

Query: 181 IGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGTV 222
           IGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGTV
Sbjct: 304 IGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGTV 345

[Close]

BLAST of Cucsat.G12338 vs. ExPASy TrEMBL
Match: A0A1S4DWP4 (polygalacturonase At1g48100 OS=Cucumis melo OX=3656 GN=LOC103490076 PE=3 SV=1)

HSP 1 Score: 465 bits (1196), Expect = 3.58e-162
Identity = 214/222 (96.40%), Postives = 220/222 (99.10%), Query Frame = 0


Query: 1   STIFTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNGLIDGRGQ 60
           STIFTGPC+NG+VFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNGLIDGRGQ
Sbjct: 113 STIFTGPCRNGIVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNGLIDGRGQ 172

Query: 61  KWWDLPCKPHKGKTMTGPCDSPIAIRFFMSSELRVEGVRIKDSPQFHFRFDNCKDVYIDS 120
           KWWDLPCKPHKGKTMTGPCDSPIAIRFFMSS+LRVEGVRIKDSPQFHFRFDNCKDVYIDS
Sbjct: 173 KWWDLPCKPHKGKTMTGPCDSPIAIRFFMSSKLRVEGVRIKDSPQFHFRFDNCKDVYIDS 232

Query: 121 LHITSPALSPNTDGIHIENTNGVQIFNSIVANGDDCVSIGSGSFNVEIRNITCGPGHGIS 180
           LHITSPALSPNTDGIHIENTNGVQIFNSIVANGDDC+SIGSGS+NVEI NITCGPGHGIS
Sbjct: 233 LHITSPALSPNTDGIHIENTNGVQIFNSIVANGDDCISIGSGSYNVEITNITCGPGHGIS 292

Query: 181 IGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGTV 222
           IGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGG+G V
Sbjct: 293 IGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGYGAV 334

[Close]

BLAST of Cucsat.G12338 vs. ExPASy TrEMBL
Match: A0A5D3DA93 (Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237G00750 PE=3 SV=1)

HSP 1 Score: 465 bits (1196), Expect = 1.13e-161
Identity = 214/222 (96.40%), Postives = 220/222 (99.10%), Query Frame = 0


Query: 1   STIFTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNGLIDGRGQ 60
           STIFTGPC+NG+VFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNGLIDGRGQ
Sbjct: 147 STIFTGPCRNGIVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNGLIDGRGQ 206

Query: 61  KWWDLPCKPHKGKTMTGPCDSPIAIRFFMSSELRVEGVRIKDSPQFHFRFDNCKDVYIDS 120
           KWWDLPCKPHKGKTMTGPCDSPIAIRFFMSS+LRVEGVRIKDSPQFHFRFDNCKDVYIDS
Sbjct: 207 KWWDLPCKPHKGKTMTGPCDSPIAIRFFMSSKLRVEGVRIKDSPQFHFRFDNCKDVYIDS 266

Query: 121 LHITSPALSPNTDGIHIENTNGVQIFNSIVANGDDCVSIGSGSFNVEIRNITCGPGHGIS 180
           LHITSPALSPNTDGIHIENTNGVQIFNSIVANGDDC+SIGSGS+NVEI NITCGPGHGIS
Sbjct: 267 LHITSPALSPNTDGIHIENTNGVQIFNSIVANGDDCISIGSGSYNVEITNITCGPGHGIS 326

Query: 181 IGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGTV 222
           IGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGG+G V
Sbjct: 327 IGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGYGAV 368

[Close]

BLAST of Cucsat.G12338 vs. ExPASy TrEMBL
Match: A0A5A7T924 (Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold36G003270 PE=3 SV=1)

HSP 1 Score: 432 bits (1112), Expect = 5.79e-149
Identity = 203/222 (91.44%), Postives = 212/222 (95.50%), Query Frame = 0


Query: 1   STIFTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNGLIDGRGQ 60
           STIFTGPC+NG+VFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNGLIDGRGQ
Sbjct: 147 STIFTGPCRNGIVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNGLIDGRGQ 206

Query: 61  KWWDLPCKPHKGKTMTGPCDSPIAIRFFMSSELRVEGVRIKDSPQFHFRFDNCKDVYIDS 120
           KWWDLPCKPHK K+++       AIRFFMSS+LRVEGVRIKDSPQFHFRFDNCKDVYIDS
Sbjct: 207 KWWDLPCKPHKSKSVSFFWQ---AIRFFMSSKLRVEGVRIKDSPQFHFRFDNCKDVYIDS 266

Query: 121 LHITSPALSPNTDGIHIENTNGVQIFNSIVANGDDCVSIGSGSFNVEIRNITCGPGHGIS 180
           LHITSPALSPNTDGIHIENTNGVQIFNSIVANGDDC+SIGSGS+NVEI NITCGPGHGIS
Sbjct: 267 LHITSPALSPNTDGIHIENTNGVQIFNSIVANGDDCISIGSGSYNVEITNITCGPGHGIS 326

Query: 181 IGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGTV 222
           IGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGG+G V
Sbjct: 327 IGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGYGAV 365

[Close]

BLAST of Cucsat.G12338 vs. ExPASy TrEMBL
Match: A0A6J1FAK7 (polygalacturonase At1g48100-like OS=Cucurbita moschata OX=3662 GN=LOC111442323 PE=3 SV=1)

HSP 1 Score: 429 bits (1103), Expect = 9.67e-148
Identity = 198/225 (88.00%), Postives = 211/225 (93.78%), Query Frame = 0


Query: 1   STIFTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNGLIDGRGQ 60
           STIFTGPCK+G+VFQVDGT +APDGPDAWPK YSAHQWLVFYRV NMSL+GNG+IDGRGQ
Sbjct: 131 STIFTGPCKHGIVFQVDGTFIAPDGPDAWPKSYSAHQWLVFYRVNNMSLRGNGVIDGRGQ 190

Query: 61  KWWDLPCKPH---KGKTMTGPCDSPIAIRFFMSSELRVEGVRIKDSPQFHFRFDNCKDVY 120
           KWWDLPCKPH   KG+T  GPCDSPIAIRFFMSS L VEG+RIKDSPQFHFRFDNC+DVY
Sbjct: 191 KWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMSSNLTVEGLRIKDSPQFHFRFDNCRDVY 250

Query: 121 IDSLHITSPALSPNTDGIHIENTNGVQIFNSIVANGDDCVSIGSGSFNVEIRNITCGPGH 180
           IDSL IT+PA SPNTDGIHIENTNGVQI NS+VANGDDC+SIGSGS+NV+IRNITCGPGH
Sbjct: 251 IDSLTITAPAWSPNTDGIHIENTNGVQILNSVVANGDDCISIGSGSYNVDIRNITCGPGH 310

Query: 181 GISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGTV 222
           GISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFG V
Sbjct: 311 GISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGAV 355

The following BLAST results are available for this feature:

BLAST of Cucsat.G12338 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q949Z1	2.4e-87	61.78	Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV...	[more]
Q94AJ5	5.0e-53	45.21	Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017...	[more]
P35336	6.5e-53	45.21	Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1	[more]
Q02096	9.4e-52	45.66	Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1	[more]
P05117	1.2e-51	45.41	Polygalacturonase-2 OS=Solanum lycopersicum OX=4081 GN=PG2 PE=1 SV=1	[more]

BLAST of Cucsat.G12338 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031737994.1	1.93e-165	100.00	polygalacturonase At1g48100 [Cucumis sativus] >KGN58717.2 hypothetical protein C...	[more]
XP_016900401.1	7.39e-162	96.40	PREDICTED: polygalacturonase At1g48100, partial [Cucumis melo]	[more]
TYK20501.1	2.33e-161	96.40	polygalacturonase [Cucumis melo var. makuwa]	[more]
XP_038905655.1	1.40e-149	89.78	polygalacturonase At1g48100-like [Benincasa hispida]	[more]
KAA0038107.1	1.20e-148	91.44	polygalacturonase [Cucumis melo var. makuwa]	[more]

BLAST of Cucsat.G12338 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LEQ6	5.82e-166	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G664600 PE=3 SV=1	[more]
A0A1S4DWP4	3.58e-162	96.40	polygalacturonase At1g48100 OS=Cucumis melo OX=3656 GN=LOC103490076 PE=3 SV=1	[more]
A0A5D3DA93	1.13e-161	96.40	Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237G00...	[more]
A0A5A7T924	5.79e-149	91.44	Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold36G003...	[more]
A0A6J1FAK7	9.67e-148	88.00	polygalacturonase At1g48100-like OS=Cucurbita moschata OX=3662 GN=LOC111442323 P...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 193..214 e-value: 20.0 score: 14.1 coord: 163..183 e-value: 2600.0 score: 4.3 coord: 113..139 e-value: 550.0 score: 9.0 coord: 140..161 e-value: 1200.0 score: 6.6
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 3..222 e-value: 1.5E-64 score: 218.2
IPR000743	Glycoside hydrolase, family 28	PROSITE	PS00502	POLYGALACTURONASE	coord: 170..183
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 1..222 e-value: 1.3E-70 score: 240.0
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 1..222
None	No IPR available	PANTHER	PTHR31375:SF119	POLYGALACTURONASE/GLYCOSIDE HYDROLASE FAMILY PROTEIN	coord: 1..222
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 3..222

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G12338.T1	Cucsat.G12338.T1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004650	polygalacturonase activity