Cucsat.G12210 (gene) Cucumber (B10) v3

Overview
NameCucsat.G12210
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Descriptionfructokinase-like 1, chloroplastic
Locationctg1837: 2239738 .. 2242087 (-)
RNA-Seq ExpressionCucsat.G12210
SyntenyCucsat.G12210
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTTAGTGGAAGAAAAGAATTGTAGGGGACGATCAGATCGAGCGGTTGACAGAGATTTTTGATGATGAAGTAGAGTTATAGCCGTCGGATGAACTTCAAGCTTTGATGACTCCGACTCTATTTAGAGAGAAAAAGACAAACCAGCTCGACAGACAGTAAATTTCTTTTAGAGGTTTTGGGTTTCAAGATAAGTTTTGTTGGCAAATTTGGATAAACCCTAACAATTGGAGCAGCCATGGCTTCATTTCATCTCCTTCCTTCGACACTTCCTCTCCCCCATGTTCAATTTTCCTGTTTTCTTCCTTTCTACTCATCAAATTACAGAGCTAAACCTATAGATTTATCTCTGATTTTCAACTTTCATCATTCACAGAGCTTGTCTAAAACGCTGAAACTAAACCCACCAAGAGCTTCCGCCAATGACGAAGGCAATGGGGTTGTTGAAACCCCCGGCCCCACTCGCCGAGGGAGGAAAAAGGGAACTGCGTCTCCATCTTCTTCTACTTCTGCTCAAAAGAAAACAAAGCGTTCTAAGGAACCCCAAATCACAGATAGAATCACAGTAGCGAACCCATTTGTTCATATCTCCGAAGACGAGTCAATCGATTCAATAGCAGATAATTACGACGATGGAATGGACATACCATACGAAGATCCTCCATTGATCTGTTGCTTCGGCGCTGCACAGAAAGAATTTGTTCCTTCAATTCGTGTACACGATAACCAAATGCATCATGATAAGTACTCGGAATGGAAAATGCTCCAATGGGATCCGCCGGAGTTTGCTAGGGCACCCGGGGGATCGCCTTCTAATGTCGCTATAGCTCACGTTAGGCTCGGTGGACGAGCTGCGTTCATGGGTAAAGTAGGGAAGGACGATTTTGGGGACGAACTCGTGTTGATGATGAACAAAGAGAAGGTACAGACTCGAGCTGTGAAGTTCGATTTGAATTCTAAAACTGCCTGTACTTACATGAAGATAAAGTTTGAAGATGGAAAGTTGAAGACTGAAACGGTGAAGGAACCGGCCGAGGATTCTCTACTTAGCTCTGAACTCAACCTTGCGGTGCTGAAAGAGGTAAATTAGCCAATGTTCATTCGAACTTGTTGTTTCATTGTTTGAGTCTAGAGATTTGTTAACAAGAGAAATTGTTTATGTTTCATCGCAGGCAAGGATCTTCCATTTCAATTCAGAAAGTTTGTTGTCCACTGCAATTGAACCAACGCTCTTCAAGGCAATCCAGTTGTCCAAGAAGTTTGGTGGACTTATCTTCTTTGATTTAAATTTGCCATTGCCGTTATGGAAATCACGCGACGAAACTCGAGAATACATTAAGAAAGCCTGGAAAGAAGCTGACATTATTGAGGTTTCAAGGCAAGAACTGGAGTTTCTCCTGGATGAAGAATACTATGAAAAGAAAAGAAACTATAGGCCTCAATACTATGCTCAAACTATTGAACAAACAAAGAATCGACGAGACCATTATCATTACACGCCGGAGGAGTTATCACCCTTGTGGCATGATCGCCTCAAGTTATTGTTCGTGACAGATGGAACATTGCGGATTCATTACTATTCTCCTTCATTTCATGGTGTGGCAATAGGTACAGAAGATGTACTCATCACACCCTTCACCTGTGATAGAACGGGTTCTGGTGATGCTGTTGTGGCTGGGATCATGAGAAAGCTTACTACTTTCCCTGAAATGCTTGAAAACCAAGATGTTTTGGAAAGGCAGCTTCGATTCGCAATCGCGGCCGGGATTATTTCTCAGTGGACAATTGGTGCAGTGAGAGGATTTCCTACCGAAAGTGCAACACAGAATCTGAAAGAGCAGGTCTATGTACCATCAATGTGGTAGGATGTAAATGTCTCTCTTGTAGCATATTGGTTTCAAAAGAAGTAAGTTTATTGGTTTTGCATTTATATAATTTGAGTTATAGAACAATCCATTGAATGTATAGTAATTCTTTCCTCTCTGGTTATCTACCTCTATTCTTAGTAAAAAGTTAGCAGTTCGGATGATATTGGAAAGATTGCTGGACTCTTTTACTAGGCATTAGAGTTGGAAGTGTATAAAACGAATACGAGAGAGAGAGAGAGAGAGAGAGAGAGAATCATGGAAGAAATGGCAATTTACCTTTCCCTTTCCAAATGTTTACATTATGAATCAAGGGGGGATAATAGCAAAGAATAACGAATGGAATGAATAATTTTTCTCCACTATTGACAGGGAAGCTGTCGGTGTAAAAACTAACCTGATTTACTATTAAAAAGAAAAACTACATCTGAACCGATTCTAATTCACCTACCAACAATCCTATTATCCATTCATTTACCTAAAAAAAT

Coding sequence (CDS)

ATGGCTTCATTTCATCTCCTTCCTTCGACACTTCCTCTCCCCCATGTTCAATTTTCCTGTTTTCTTCCTTTCTACTCATCAAATTACAGAGCTAAACCTATAGATTTATCTCTGATTTTCAACTTTCATCATTCACAGAGCTTGTCTAAAACGCTGAAACTAAACCCACCAAGAGCTTCCGCCAATGACGAAGGCAATGGGGTTGTTGAAACCCCCGGCCCCACTCGCCGAGGGAGGAAAAAGGGAACTGCGTCTCCATCTTCTTCTACTTCTGCTCAAAAGAAAACAAAGCGTTCTAAGGAACCCCAAATCACAGATAGAATCACAGTAGCGAACCCATTTGTTCATATCTCCGAAGACGAGTCAATCGATTCAATAGCAGATAATTACGACGATGGAATGGACATACCATACGAAGATCCTCCATTGATCTGTTGCTTCGGCGCTGCACAGAAAGAATTTGTTCCTTCAATTCGTGTACACGATAACCAAATGCATCATGATAAGTACTCGGAATGGAAAATGCTCCAATGGGATCCGCCGGAGTTTGCTAGGGCACCCGGGGGATCGCCTTCTAATGTCGCTATAGCTCACGTTAGGCTCGGTGGACGAGCTGCGTTCATGGGTAAAGTAGGGAAGGACGATTTTGGGGACGAACTCGTGTTGATGATGAACAAAGAGAAGGTACAGACTCGAGCTGTGAAGTTCGATTTGAATTCTAAAACTGCCTGTACTTACATGAAGATAAAGTTTGAAGATGGAAAGTTGAAGACTGAAACGGTGAAGGAACCGGCCGAGGATTCTCTACTTAGCTCTGAACTCAACCTTGCGGTGCTGAAAGAGGCAAGGATCTTCCATTTCAATTCAGAAAGTTTGTTGTCCACTGCAATTGAACCAACGCTCTTCAAGGCAATCCAGTTGTCCAAGAAGTTTGGTGGACTTATCTTCTTTGATTTAAATTTGCCATTGCCGTTATGGAAATCACGCGACGAAACTCGAGAATACATTAAGAAAGCCTGGAAAGAAGCTGACATTATTGAGGTTTCAAGGCAAGAACTGGAGTTTCTCCTGGATGAAGAATACTATGAAAAGAAAAGAAACTATAGGCCTCAATACTATGCTCAAACTATTGAACAAACAAAGAATCGACGAGACCATTATCATTACACGCCGGAGGAGTTATCACCCTTGTGGCATGATCGCCTCAAGTTATTGTTCGTGACAGATGGAACATTGCGGATTCATTACTATTCTCCTTCATTTCATGGTGTGGCAATAGGTACAGAAGATGTACTCATCACACCCTTCACCTGTGATAGAACGGGTTCTGGTGATGCTGTTGTGGCTGGGATCATGAGAAAGCTTACTACTTTCCCTGAAATGCTTGAAAACCAAGATGTTTTGGAAAGGCAGCTTCGATTCGCAATCGCGGCCGGGATTATTTCTCAGTGGACAATTGGTGCAGTGAGAGGATTTCCTACCGAAAGTGCAACACAGAATCTGAAAGAGCAGGTCTATGTACCATCAATGTGGTAG

Protein sequence

MASFHLLPSTLPLPHVQFSCFLPFYSSNYRAKPIDLSLIFNFHHSQSLSKTLKLNPPRASANDEGNGVVETPGPTRRGRKKGTASPSSSTSAQKKTKRSKEPQITDRITVANPFVHISEDESIDSIADNYDDGMDIPYEDPPLICCFGAAQKEFVPSIRVHDNQMHHDKYSEWKMLQWDPPEFARAPGGSPSNVAIAHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDLNSKTACTYMKIKFEDGKLKTETVKEPAEDSLLSSELNLAVLKEARIFHFNSESLLSTAIEPTLFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWKEADIIEVSRQELEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEELSPLWHDRLKLLFVTDGTLRIHYYSPSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMLENQDVLERQLRFAIAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW
Homology
BLAST of Cucsat.G12210 vs. ExPASy Swiss-Prot
Match: Q9M394 (Fructokinase-like 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FLN1 PE=1 SV=1)

HSP 1 Score: 663.3 bits (1710), Expect = 2.2e-189
Identity = 339/482 (70.33%), Postives = 386/482 (80.08%), Query Frame = 0

Query: 34  IDLSLIFNFHHSQSLSKTLKLNPPRASANDEG--NGVV--ETPGPTRRGRKKGTASPSSS 93
           +D   +    HSQ+  + L    P+AS N  G  NG     T  P+R+GRKK        
Sbjct: 17  LDRREVLVVRHSQASRRFL---TPKASINGSGITNGAAAETTSKPSRKGRKK-------- 76

Query: 94  TSAQKKTKRSKEPQITDRITVANPFVHISEDESIDSIADNYDDGMDIPYEDPPLICCFGA 153
               K+T    E   T+     NP            +AD YDDG++ PY+DPPL+CCFGA
Sbjct: 77  ----KQTSTVIEKDNTETDPELNP-----------ELAD-YDDGIEFPYDDPPLVCCFGA 136

Query: 154 AQKEFVPSIRVHDNQMHHDKYSEWKMLQWDPPEFARAPGGSPSNVAIAHVRLGGRAAFMG 213
            QKEFVP +RVHDN MH D YS+WKMLQWDPPEF RAPGG PSNVAI+HVRLGGRAAFMG
Sbjct: 137 VQKEFVPVVRVHDNPMHPDMYSQWKMLQWDPPEFGRAPGGPPSNVAISHVRLGGRAAFMG 196

Query: 214 KVGKDDFGDELVLMMNKEKVQTRAVKFDLNSKTACTYMKIKFEDGKLKTETVKEPAEDSL 273
           KVG+DDFGDELVLMMN+E+VQTRAVKFD NSKTACT +KIKF+DGK+  ETVKEP EDSL
Sbjct: 197 KVGEDDFGDELVLMMNQERVQTRAVKFDENSKTACTRVKIKFKDGKMMAETVKEPPEDSL 256

Query: 274 LSSELNLAVLKEARIFHFNSESLLSTAIEPTLFKAIQLSKKFGGLIFFDLNLPLPLWKSR 333
            +SELNLAVLKEARIFHFNSE L S  ++ TLF AIQ SKKFGGLIFFDLNLPLPLW+SR
Sbjct: 257 FASELNLAVLKEARIFHFNSEVLTSPTMQSTLFTAIQWSKKFGGLIFFDLNLPLPLWRSR 316

Query: 334 DETREYIKKAWKEADIIEVSRQELEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHY 393
           +ETR+ IKKAW EA+IIEVS+QELEFLLDE+YYE++RNY PQY+A+  +QTKNRRD+YHY
Sbjct: 317 NETRKLIKKAWNEANIIEVSQQELEFLLDEDYYERRRNYTPQYFAEDFDQTKNRRDYYHY 376

Query: 394 TPEELSPLWHDRLKLLFVTDGTLRIHYYSPSFHGVAIGTEDVLITPFTCDRTGSGDAVVA 453
           TPEE+  LWHD+LKLL VTDGTLR+HYY+P+F GV IGTEDVLITPFTCDRTGSGDAVVA
Sbjct: 377 TPEEIKSLWHDKLKLLVVTDGTLRLHYYTPTFDGVVIGTEDVLITPFTCDRTGSGDAVVA 436

Query: 454 GIMRKLTTFPEMLENQDVLERQLRFAIAAGIISQWTIGAVRGFPTESATQNLKEQVYVPS 512
           GIMRKLTT PEM E+QDV+ERQLRFA+AAGII+QWTIGAVRGFPTESATQNLKEQVYVPS
Sbjct: 437 GIMRKLTTCPEMFEDQDVMERQLRFAVAAGIIAQWTIGAVRGFPTESATQNLKEQVYVPS 471

BLAST of Cucsat.G12210 vs. ExPASy Swiss-Prot
Match: F4I0K2 (Fructokinase-like 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FLN2 PE=1 SV=2)

HSP 1 Score: 321.6 bits (823), Expect = 1.6e-86
Identity = 177/425 (41.65%), Postives = 256/425 (60.24%), Query Frame = 0

Query: 69  VETPGPTRRGRKKGTASPSSSTSAQKKTKRSKEPQITDRITVANPFVHISEDESIDSIAD 128
           VE     ++ R+K T            T    E    +           SE+E ID ++ 
Sbjct: 133 VEEVKTEKKVRRKRTVKKDKDVEDDLATIMDAEVSDVEEALAVESTDTESEEEEID-LSK 192

Query: 129 NYDDGMDIPYEDPPLICCFGAAQKEFVPSIRVHDNQMHHDKYSEWKMLQWDPPEFARAPG 188
           +  + +   Y  PPL+CCFG+AQ  FVPS R  +  + ++ +   +  +W P ++ RAPG
Sbjct: 193 HEGEDISHTYGWPPLVCCFGSAQHAFVPSGRPANRLLDYELHERMRDAKWAPEKYIRAPG 252

Query: 189 GSPSNVAIAHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDLNSKTACTYMK 248
           G    VAIA   LGG+ AFMGK+G DD+G  ++  +N  KVQTR+VK D    TAC+ MK
Sbjct: 253 GCAGGVAIALASLGGKVAFMGKLGADDYGQAMLYYLNVCKVQTRSVKIDGKRVTACSTMK 312

Query: 249 IKFEDGKLKTETVKEPAEDSLLSSELNLAVLKEARIFHFNSESLLSTAIEPTLFKAIQLS 308
           I  + G+LK+  +K  AEDSL  SE+N+ VLKEA++F+F++ SLL   +  T  +AI++S
Sbjct: 313 IS-KRGRLKSTCIKPCAEDSLSKSEINVDVLKEAKMFYFSTHSLLDKKMMSTTIQAIKIS 372

Query: 309 KKFGGLIFFDLNLPLPLWKSRDETREYIKKAWKEADIIEVSRQELEFLLDEEYYEKKRNY 368
           K+ G +IF+DLNLPLPLW S +ET+ +I++ W  AD+IE+++QELEFL   E  E     
Sbjct: 373 KQLGNVIFYDLNLPLPLWHSSEETKSFIQEVWNLADVIEITKQELEFLCGIEPTE----- 432

Query: 369 RPQYYAQTIEQTKNRRDHYHYTPEELSPLWHDRLKLLFVTDGTLRIHYYSPSFHGVAIGT 428
             ++  +  + +K      HY PE +  LWH+ LK+LFVT+GT +IHYY+   +G   G 
Sbjct: 433 --EFDTENNDISK----FVHYPPETVEQLWHENLKVLFVTNGTSKIHYYTKEHNGAVSGM 492

Query: 429 EDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMLENQDVLERQLRFAIAAGIISQWTIGA 488
           EDV ITPFT D + SGD +VAG++R LT  P+++ N+  LER  R+AI  GII QW +  
Sbjct: 493 EDVPITPFTRDMSASGDGIVAGLIRMLTVQPDLMNNKGYLERTARYAIECGIIDQWLLAQ 544

Query: 489 VRGFP 494
            RG+P
Sbjct: 553 TRGYP 544

BLAST of Cucsat.G12210 vs. ExPASy Swiss-Prot
Match: Q42896 (Fructokinase-2 OS=Solanum lycopersicum OX=4081 GN=FRK2 PE=2 SV=2)

HSP 1 Score: 193.7 bits (491), Expect = 5.0e-48
Identity = 116/324 (35.80%), Postives = 174/324 (53.70%), Query Frame = 0

Query: 179 DPPEFARAPGGSPSNVAIAHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDL 238
           + P F +APGG+P+NVAIA  RLGG++AF+GK+G D+FG  L  ++    VQ   + FD 
Sbjct: 33  EAPGFLKAPGGAPANVAIAVTRLGGKSAFVGKLGDDEFGHMLAGILKTNGVQAEGINFDK 92

Query: 239 NSKTACTYMKIKFEDGKLKTETVKEPAEDSLLS-SELNLAVLKEARIFHFNSESLLSTAI 298
            ++TA  ++ ++  DG+ +    + P+ D LL+ +ELNL +++ A++FH+ S SL+    
Sbjct: 93  GARTALAFVTLR-ADGEREFMFYRNPSADMLLTPAELNLDLIRSAKVFHYGSISLIVEPC 152

Query: 299 EPTLFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWKEADIIEVSRQELEFLL 358
                KA++++K+ G L+ +D NL LPLW S +E ++ IK  W  AD+I+VS  ELEFL 
Sbjct: 153 RAAHMKAMEVAKEAGALLSYDPNLRLPLWPSAEEAKKQIKSIWDSADVIKVSDVELEFLT 212

Query: 359 DEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEELSPLWHDRLKLLFVTDGTLRIHYY 418
                                   N+ D      E    LWH  LKLL VT G    +YY
Sbjct: 213 G----------------------SNKID-----DESAMSLWHPNLKLLLVTLGEKGCNYY 272

Query: 419 SPSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMLENQDVLERQLRFAIA 478
           +  FHG   G         T D TG+GD+ V  ++ K+     +LE++  L+  LRF+ A
Sbjct: 273 TKKFHGTVGG-----FHVKTVDTTGAGDSFVGALLTKIVDDQTILEDEARLKEVLRFSCA 323

Query: 479 AGIISQWTIGAVRGFPTESATQNL 502
            G I+    GA+   PT S    L
Sbjct: 333 CGAITTTKKGAIPALPTASEALTL 323

BLAST of Cucsat.G12210 vs. ExPASy Swiss-Prot
Match: Q7XJ81 (Fructokinase-2 OS=Solanum habrochaites OX=62890 GN=FRK2 PE=2 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 1.9e-47
Identity = 116/324 (35.80%), Postives = 173/324 (53.40%), Query Frame = 0

Query: 179 DPPEFARAPGGSPSNVAIAHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDL 238
           + P F +APGG+P+NVAIA  RLGGR+AF+GK+G D+FG  L  ++    VQ   + FD 
Sbjct: 33  EAPGFLKAPGGAPANVAIAVTRLGGRSAFVGKLGDDEFGHMLAGILKTNGVQADGINFDK 92

Query: 239 NSKTACTYMKIKFEDGKLKTETVKEPAEDSLLS-SELNLAVLKEARIFHFNSESLLSTAI 298
            ++TA  ++ ++  DG+ +    + P+ D LL+ +ELNL +++ A++FH+ S SL+    
Sbjct: 93  GARTALAFVTLR-ADGEREFMFYRNPSADMLLTPAELNLDLIRSAKVFHYGSISLIVEPC 152

Query: 299 EPTLFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWKEADIIEVSRQELEFLL 358
                KA++++K+ G L+ +D NL LPLW S +E ++ IK  W  AD+I+VS  ELEFL 
Sbjct: 153 RAAHMKAMEVAKEAGALLSYDPNLRLPLWPSAEEAKKQIKSIWDSADVIKVSDVELEFLT 212

Query: 359 DEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEELSPLWHDRLKLLFVTDGTLRIHYY 418
                                   N+ D      E    LWH  LKLL VT G    +YY
Sbjct: 213 G----------------------SNKID-----DESAMSLWHPNLKLLLVTLGEKGCNYY 272

Query: 419 SPSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMLENQDVLERQLRFAIA 478
           +  FHG   G         T D TG+GD+ V  ++ K+     +L ++  L+  LRF+ A
Sbjct: 273 TKKFHGTVGG-----FHVKTVDTTGAGDSFVGALLTKIVDDQTILADEARLKEVLRFSCA 323

Query: 479 AGIISQWTIGAVRGFPTESATQNL 502
            G I+    GA+   PT S    L
Sbjct: 333 CGAITTTKKGAIPALPTASEALTL 323

BLAST of Cucsat.G12210 vs. ExPASy Swiss-Prot
Match: Q9M1B9 (Probable fructokinase-4 OS=Arabidopsis thaliana OX=3702 GN=At3g59480 PE=2 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 5.5e-47
Identity = 116/327 (35.47%), Postives = 173/327 (52.91%), Query Frame = 0

Query: 179 DPPEFARAPGGSPSNVAIAHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDL 238
           D P F +APGG+P+NVAIA  RLGGRAAF+GK+G D+FG  L  ++ +  V    + FD 
Sbjct: 32  DAPGFIKAPGGAPANVAIAISRLGGRAAFVGKLGDDEFGHMLAGILKQNGVSAEGINFDT 91

Query: 239 NSKTACTYMKIKFEDGKLKTETVKEPAEDSLL-SSELNLAVLKEARIFHFNSESLLSTAI 298
            ++TA  ++ ++  DG+ +    + P+ D LL   ELNL V++ A++FH+ S SL+    
Sbjct: 92  GARTALAFVTLR-SDGEREFMFYRNPSADMLLRPDELNLDVIRSAKVFHYGSISLIVEPC 151

Query: 299 EPTLFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWKEADIIEVSRQELEFLL 358
                KA++++K+ G L+ +D NL LPLW S++E ++ I   W +A++I+VS +EL FL 
Sbjct: 152 RSAHLKAMEVAKEAGALLSYDPNLRLPLWPSKEEAQKQILSIWDKAEVIKVSDEELMFLT 211

Query: 359 DEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEELSPLWHDRLKLLFVTDGTLRIHYY 418
             +  +                            E    LWH  LKLL VT G     YY
Sbjct: 212 GSDKVD---------------------------DETALSLWHSNLKLLLVTLGEKGCRYY 271

Query: 419 SPSFHGVAIGTEDVLITPF---TCDRTGSGDAVVAGIMRKLTTFPEMLENQDVLERQLRF 478
           + SF G         + PF     D TG+GD+ V  ++ K+     +LE++  L   LR 
Sbjct: 272 TKSFRG--------SVDPFHVDAVDTTGAGDSFVGALLCKIVDDRAVLEDEARLREVLRL 322

Query: 479 AIAAGIISQWTIGAVRGFPTESATQNL 502
           A A G I+    GA+   PTES  Q+L
Sbjct: 332 ANACGAITTTKKGAIPALPTESEVQSL 322

BLAST of Cucsat.G12210 vs. NCBI nr
Match: XP_031737955.1 (fructokinase-like 1, chloroplastic [Cucumis sativus] >KGN58805.1 hypothetical protein Csa_000812 [Cucumis sativus])

HSP 1 Score: 1021 bits (2639), Expect = 0.0
Identity = 511/511 (100.00%), Postives = 511/511 (100.00%), Query Frame = 0

Query: 1   MASFHLLPSTLPLPHVQFSCFLPFYSSNYRAKPIDLSLIFNFHHSQSLSKTLKLNPPRAS 60
           MASFHLLPSTLPLPHVQFSCFLPFYSSNYRAKPIDLSLIFNFHHSQSLSKTLKLNPPRAS
Sbjct: 1   MASFHLLPSTLPLPHVQFSCFLPFYSSNYRAKPIDLSLIFNFHHSQSLSKTLKLNPPRAS 60

Query: 61  ANDEGNGVVETPGPTRRGRKKGTASPSSSTSAQKKTKRSKEPQITDRITVANPFVHISED 120
           ANDEGNGVVETPGPTRRGRKKGTASPSSSTSAQKKTKRSKEPQITDRITVANPFVHISED
Sbjct: 61  ANDEGNGVVETPGPTRRGRKKGTASPSSSTSAQKKTKRSKEPQITDRITVANPFVHISED 120

Query: 121 ESIDSIADNYDDGMDIPYEDPPLICCFGAAQKEFVPSIRVHDNQMHHDKYSEWKMLQWDP 180
           ESIDSIADNYDDGMDIPYEDPPLICCFGAAQKEFVPSIRVHDNQMHHDKYSEWKMLQWDP
Sbjct: 121 ESIDSIADNYDDGMDIPYEDPPLICCFGAAQKEFVPSIRVHDNQMHHDKYSEWKMLQWDP 180

Query: 181 PEFARAPGGSPSNVAIAHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDLNS 240
           PEFARAPGGSPSNVAIAHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDLNS
Sbjct: 181 PEFARAPGGSPSNVAIAHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDLNS 240

Query: 241 KTACTYMKIKFEDGKLKTETVKEPAEDSLLSSELNLAVLKEARIFHFNSESLLSTAIEPT 300
           KTACTYMKIKFEDGKLKTETVKEPAEDSLLSSELNLAVLKEARIFHFNSESLLSTAIEPT
Sbjct: 241 KTACTYMKIKFEDGKLKTETVKEPAEDSLLSSELNLAVLKEARIFHFNSESLLSTAIEPT 300

Query: 301 LFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWKEADIIEVSRQELEFLLDEE 360
           LFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWKEADIIEVSRQELEFLLDEE
Sbjct: 301 LFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWKEADIIEVSRQELEFLLDEE 360

Query: 361 YYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEELSPLWHDRLKLLFVTDGTLRIHYYSPS 420
           YYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEELSPLWHDRLKLLFVTDGTLRIHYYSPS
Sbjct: 361 YYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEELSPLWHDRLKLLFVTDGTLRIHYYSPS 420

Query: 421 FHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMLENQDVLERQLRFAIAAGI 480
           FHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMLENQDVLERQLRFAIAAGI
Sbjct: 421 FHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMLENQDVLERQLRFAIAAGI 480

Query: 481 ISQWTIGAVRGFPTESATQNLKEQVYVPSMW 511
           ISQWTIGAVRGFPTESATQNLKEQVYVPSMW
Sbjct: 481 ISQWTIGAVRGFPTESATQNLKEQVYVPSMW 511

BLAST of Cucsat.G12210 vs. NCBI nr
Match: XP_016899692.1 (PREDICTED: fructokinase-like 1, chloroplastic [Cucumis melo] >KAA0044141.1 fructokinase-like 1 [Cucumis melo var. makuwa] >TYK24996.1 fructokinase-like 1 [Cucumis melo var. makuwa])

HSP 1 Score: 976 bits (2523), Expect = 0.0
Identity = 490/511 (95.89%), Postives = 495/511 (96.87%), Query Frame = 0

Query: 1   MASFHLLPSTLPLPHVQFSCFLPFYSSNYRAKPIDLSLIFNFHHSQSLSKTLKLNPPRAS 60
           MASFHLLPSTLPLPHVQFSCFLPFYSS YRAKPIDLSLIFN  H QS SKTLKLNPPRAS
Sbjct: 1   MASFHLLPSTLPLPHVQFSCFLPFYSSTYRAKPIDLSLIFNLRHFQSSSKTLKLNPPRAS 60

Query: 61  ANDEGNGVVETPGPTRRGRKKGTASPSSSTSAQKKTKRSKEPQITDRITVANPFVHISED 120
            N EGNGVVETP PTRRGRKKGTASPSSSTSAQKKTKRSK+PQITD+ TVANPF HISED
Sbjct: 61  TNGEGNGVVETPSPTRRGRKKGTASPSSSTSAQKKTKRSKKPQITDKTTVANPFDHISED 120

Query: 121 ESIDSIADNYDDGMDIPYEDPPLICCFGAAQKEFVPSIRVHDNQMHHDKYSEWKMLQWDP 180
           ESIDSIADNYDDGMDIPYEDPPLICCFGAAQKEFVPSIRVHDNQM  DKYSEWKMLQWDP
Sbjct: 121 ESIDSIADNYDDGMDIPYEDPPLICCFGAAQKEFVPSIRVHDNQMDADKYSEWKMLQWDP 180

Query: 181 PEFARAPGGSPSNVAIAHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDLNS 240
           PEFARAPGGSPSNVAIAHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDLNS
Sbjct: 181 PEFARAPGGSPSNVAIAHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDLNS 240

Query: 241 KTACTYMKIKFEDGKLKTETVKEPAEDSLLSSELNLAVLKEARIFHFNSESLLSTAIEPT 300
           KTACTYMKIKFEDGKLK ETVKEPAEDSLLSSELNLAVLKEARIFHFNSE+LLST IEPT
Sbjct: 241 KTACTYMKIKFEDGKLKMETVKEPAEDSLLSSELNLAVLKEARIFHFNSEALLSTVIEPT 300

Query: 301 LFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWKEADIIEVSRQELEFLLDEE 360
           LFKAIQLSKKFGGLIFFDLNLPLPLWKSR+ETREYIKKAW EADIIEVSRQELEFLLDEE
Sbjct: 301 LFKAIQLSKKFGGLIFFDLNLPLPLWKSREETREYIKKAWNEADIIEVSRQELEFLLDEE 360

Query: 361 YYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEELSPLWHDRLKLLFVTDGTLRIHYYSPS 420
           YYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEE+SPLWHD LKLLFVTDGTLRIHYYSPS
Sbjct: 361 YYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEEISPLWHDHLKLLFVTDGTLRIHYYSPS 420

Query: 421 FHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMLENQDVLERQLRFAIAAGI 480
           FHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMLENQDVLERQLRFAIAAGI
Sbjct: 421 FHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMLENQDVLERQLRFAIAAGI 480

Query: 481 ISQWTIGAVRGFPTESATQNLKEQVYVPSMW 511
           ISQWTIGAVRGFPTESATQNLKEQVYVPSMW
Sbjct: 481 ISQWTIGAVRGFPTESATQNLKEQVYVPSMW 511

BLAST of Cucsat.G12210 vs. NCBI nr
Match: XP_038906230.1 (fructokinase-like 1, chloroplastic [Benincasa hispida])

HSP 1 Score: 932 bits (2409), Expect = 0.0
Identity = 468/511 (91.59%), Postives = 483/511 (94.52%), Query Frame = 0

Query: 1   MASFHLLPSTLPLPHVQFSCFLPFYSSNYRAKPIDLSLIFNFHHSQSLSKTLKLNPPRAS 60
           MASF LLPSTL LPHVQFSCFLPFYSSNYRAK +DLSLIF  HH QS S++LK N PRAS
Sbjct: 1   MASFQLLPSTLSLPHVQFSCFLPFYSSNYRAKTLDLSLIFKSHHLQSSSQSLKQNTPRAS 60

Query: 61  ANDEGNGVVETPGPTRRGRKKGTASPSSSTSAQKKTKRSKEPQITDRITVANPFVHISED 120
            N +GNGVVETP PTRRGRKKGT SPSSS S QKKTKRSK+PQITD  T  NPF H SED
Sbjct: 61  VNGDGNGVVETPSPTRRGRKKGTTSPSSS-STQKKTKRSKKPQITDETTEGNPFDHASED 120

Query: 121 ESIDSIADNYDDGMDIPYEDPPLICCFGAAQKEFVPSIRVHDNQMHHDKYSEWKMLQWDP 180
           E IDSIADNYDDGMDIPYEDPPLICCFGAAQKEFVPSIRVHDNQMH DKYSEWKMLQWDP
Sbjct: 121 EPIDSIADNYDDGMDIPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSEWKMLQWDP 180

Query: 181 PEFARAPGGSPSNVAIAHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDLNS 240
           PEFARAPGGSPSNVAI+HVRLGGRAAFMGKVGKDDFGDE+VLMMNKEKVQTRAVKFDLNS
Sbjct: 181 PEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDEVVLMMNKEKVQTRAVKFDLNS 240

Query: 241 KTACTYMKIKFEDGKLKTETVKEPAEDSLLSSELNLAVLKEARIFHFNSESLLSTAIEPT 300
           +TACTYMKIKFEDG+LK ETVKEPAEDSL+SSELNLAVLKEARIFHFNSE+LLSTA++ T
Sbjct: 241 RTACTYMKIKFEDGRLKMETVKEPAEDSLVSSELNLAVLKEARIFHFNSEALLSTAMKST 300

Query: 301 LFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWKEADIIEVSRQELEFLLDEE 360
           LFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAW EADIIEVSRQELEFLLDEE
Sbjct: 301 LFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWNEADIIEVSRQELEFLLDEE 360

Query: 361 YYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEELSPLWHDRLKLLFVTDGTLRIHYYSPS 420
           YYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEE+SPLWHDRLKLLFVTDGTLRIHYYSPS
Sbjct: 361 YYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEEISPLWHDRLKLLFVTDGTLRIHYYSPS 420

Query: 421 FHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMLENQDVLERQLRFAIAAGI 480
           FHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPE+L NQD LERQLRFAIAAGI
Sbjct: 421 FHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEILVNQDALERQLRFAIAAGI 480

Query: 481 ISQWTIGAVRGFPTESATQNLKEQVYVPSMW 511
           ISQWTIGAVRGFPTESATQNLKEQVYVPSMW
Sbjct: 481 ISQWTIGAVRGFPTESATQNLKEQVYVPSMW 510

BLAST of Cucsat.G12210 vs. NCBI nr
Match: XP_022145754.1 (fructokinase-like 1, chloroplastic [Momordica charantia])

HSP 1 Score: 858 bits (2217), Expect = 2.81e-311
Identity = 435/517 (84.14%), Postives = 463/517 (89.56%), Query Frame = 0

Query: 1   MASFHLLPSTLPLPHVQFSCFLPF-----YSSNYRAKPIDLSLIFNFHHSQSLSKTLKLN 60
           MASF LLP+T+ LPH+ FS FL       Y +     P+D  LIFN  + Q+ SK+ +LN
Sbjct: 1   MASFQLLPATISLPHLHFSRFLSNPSHCRYQTTELLNPLDSFLIFNSRYVQNSSKSPRLN 60

Query: 61  PPRASANDEGNGVVETPGPTRRGRKKG-TASPSSSTSAQKKTKRSKEPQITDRITVANPF 120
           P RAS  D+GNGVVET  PTRRGRKKG T S SSST+ QKKTKRSK+  + D  T  NPF
Sbjct: 61  PARASVGDDGNGVVETASPTRRGRKKGMTKSSSSSTTTQKKTKRSKKTLVEDETTEGNPF 120

Query: 121 VHISEDESIDSIADNYDDGMDIPYEDPPLICCFGAAQKEFVPSIRVHDNQMHHDKYSEWK 180
             +SEDE IDSIA+ YDDGMD PYEDPPLICCFGAAQKEFVPS+RVHDNQMH DKYSEWK
Sbjct: 121 EQVSEDEPIDSIAEKYDDGMDFPYEDPPLICCFGAAQKEFVPSVRVHDNQMHADKYSEWK 180

Query: 181 MLQWDPPEFARAPGGSPSNVAIAHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAV 240
           MLQWDPPEFARAPGG+PSNVAI+HVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAV
Sbjct: 181 MLQWDPPEFARAPGGAPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAV 240

Query: 241 KFDLNSKTACTYMKIKFEDGKLKTETVKEPAEDSLLSSELNLAVLKEARIFHFNSESLLS 300
           KFDLNS+TACTYMKIKFEDGKLK ETVKE AEDSLLSSELNL+VLKEARIFHFNSE+L+S
Sbjct: 241 KFDLNSRTACTYMKIKFEDGKLKMETVKESAEDSLLSSELNLSVLKEARIFHFNSEALMS 300

Query: 301 TAIEPTLFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWKEADIIEVSRQELE 360
           TA+E TLFKAIQLSKKFGGLIFFDLNL LPLW+SRDETREYIKKAW EADIIEVSRQELE
Sbjct: 301 TAMESTLFKAIQLSKKFGGLIFFDLNLLLPLWRSRDETREYIKKAWNEADIIEVSRQELE 360

Query: 361 FLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEELSPLWHDRLKLLFVTDGTLRI 420
           FLLDEEYYEKKRNYRPQYYAQTIEQTKNRRD+YHYTPEE+SPLWHDRL+LLFVTDGT+RI
Sbjct: 361 FLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYTPEEISPLWHDRLRLLFVTDGTMRI 420

Query: 421 HYYSPSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMLENQDVLERQLRF 480
           HYYSPSFHGV IGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEM ENQD LERQLRF
Sbjct: 421 HYYSPSFHGVVIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFENQDXLERQLRF 480

Query: 481 AIAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 511
           AIAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW
Sbjct: 481 AIAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 517

BLAST of Cucsat.G12210 vs. NCBI nr
Match: KAG7027918.1 (Fructokinase-like 1, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 834 bits (2155), Expect = 4.42e-302
Identity = 428/513 (83.43%), Postives = 456/513 (88.89%), Query Frame = 0

Query: 1   MASFHLLPSTLPLPHVQFSCFLPFYSSNYRAKPIDLSLIFNFHHSQSLSKTLKLNPPRAS 60
           MASF LLP+T  LPH+QFS FL FY S +R K    +LIFN  + QS  K+LKL+PP AS
Sbjct: 1   MASFQLLPATFSLPHLQFSRFLHFYPSIFREK----TLIFNSRYLQSSCKSLKLSPPGAS 60

Query: 61  ANDEGNGVVETPGPTRRGRKKGTASPSSSTSAQKKTKRSKEPQITDRITVANPF--VHIS 120
            N +GN    TP PTRRGRKKGT SPSSS      TKRS++  I D  T  N F  + + 
Sbjct: 61  -NADGNEDTGTPTPTRRGRKKGTKSPSSS------TKRSRKTLIEDEPTQGNLFDQMSVD 120

Query: 121 EDESIDSIADNYDDGMDIPYEDPPLICCFGAAQKEFVPSIRVHDNQMHHDKYSEWKMLQW 180
           EDE+IDSIA+NYDDGMD PYEDPPLICCFGAAQKEFVPSIRVHDNQMH DKYSEWKMLQW
Sbjct: 121 EDEAIDSIAENYDDGMDFPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSEWKMLQW 180

Query: 181 DPPEFARAPGGSPSNVAIAHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDL 240
           DPPEFARAPGGSPSNVAI+HVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTR VKFDL
Sbjct: 181 DPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRGVKFDL 240

Query: 241 NSKTACTYMKIKFEDGKLKTETVKEPAEDSLLSSELNLAVLKEARIFHFNSESLLSTAIE 300
           NS+TACTYMKIKF+DGKLK ETVKE AEDSL+SSELNLAVLKEARIFHFNSE+LLST +E
Sbjct: 241 NSRTACTYMKIKFDDGKLKMETVKESAEDSLVSSELNLAVLKEARIFHFNSEALLSTTME 300

Query: 301 PTLFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWKEADIIEVSRQELEFLLD 360
            TLF AIQLSKKFGGLIFFDLNLPLPLW+SRDETR+YI+KAW +ADIIEVSRQELEFLLD
Sbjct: 301 ATLFNAIQLSKKFGGLIFFDLNLPLPLWRSRDETRKYIEKAWNQADIIEVSRQELEFLLD 360

Query: 361 EEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEELSPLWHDRLKLLFVTDGTLRIHYYS 420
           EEYYEKKRNYRPQYYAQTIEQTKNRRDHYHY+PEE+SPLWHD LKLL +TDGTLRIHYYS
Sbjct: 361 EEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYSPEEISPLWHDHLKLLLITDGTLRIHYYS 420

Query: 421 PSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMLENQDVLERQLRFAIAA 480
           PSFHGV IGTEDVLITPFTCDRTGSGDA+VAGIMRKLTTFPEM ENQDVLERQLRFAIAA
Sbjct: 421 PSFHGVVIGTEDVLITPFTCDRTGSGDALVAGIMRKLTTFPEMFENQDVLERQLRFAIAA 480

Query: 481 GIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 511
           GIISQWTIGAVRGFPTESATQNLKEQVYVPSMW
Sbjct: 481 GIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 502

BLAST of Cucsat.G12210 vs. ExPASy TrEMBL
Match: A0A0A0LA03 (PfkB domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G732620 PE=4 SV=1)

HSP 1 Score: 1021 bits (2639), Expect = 0.0
Identity = 511/511 (100.00%), Postives = 511/511 (100.00%), Query Frame = 0

Query: 1   MASFHLLPSTLPLPHVQFSCFLPFYSSNYRAKPIDLSLIFNFHHSQSLSKTLKLNPPRAS 60
           MASFHLLPSTLPLPHVQFSCFLPFYSSNYRAKPIDLSLIFNFHHSQSLSKTLKLNPPRAS
Sbjct: 1   MASFHLLPSTLPLPHVQFSCFLPFYSSNYRAKPIDLSLIFNFHHSQSLSKTLKLNPPRAS 60

Query: 61  ANDEGNGVVETPGPTRRGRKKGTASPSSSTSAQKKTKRSKEPQITDRITVANPFVHISED 120
           ANDEGNGVVETPGPTRRGRKKGTASPSSSTSAQKKTKRSKEPQITDRITVANPFVHISED
Sbjct: 61  ANDEGNGVVETPGPTRRGRKKGTASPSSSTSAQKKTKRSKEPQITDRITVANPFVHISED 120

Query: 121 ESIDSIADNYDDGMDIPYEDPPLICCFGAAQKEFVPSIRVHDNQMHHDKYSEWKMLQWDP 180
           ESIDSIADNYDDGMDIPYEDPPLICCFGAAQKEFVPSIRVHDNQMHHDKYSEWKMLQWDP
Sbjct: 121 ESIDSIADNYDDGMDIPYEDPPLICCFGAAQKEFVPSIRVHDNQMHHDKYSEWKMLQWDP 180

Query: 181 PEFARAPGGSPSNVAIAHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDLNS 240
           PEFARAPGGSPSNVAIAHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDLNS
Sbjct: 181 PEFARAPGGSPSNVAIAHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDLNS 240

Query: 241 KTACTYMKIKFEDGKLKTETVKEPAEDSLLSSELNLAVLKEARIFHFNSESLLSTAIEPT 300
           KTACTYMKIKFEDGKLKTETVKEPAEDSLLSSELNLAVLKEARIFHFNSESLLSTAIEPT
Sbjct: 241 KTACTYMKIKFEDGKLKTETVKEPAEDSLLSSELNLAVLKEARIFHFNSESLLSTAIEPT 300

Query: 301 LFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWKEADIIEVSRQELEFLLDEE 360
           LFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWKEADIIEVSRQELEFLLDEE
Sbjct: 301 LFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWKEADIIEVSRQELEFLLDEE 360

Query: 361 YYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEELSPLWHDRLKLLFVTDGTLRIHYYSPS 420
           YYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEELSPLWHDRLKLLFVTDGTLRIHYYSPS
Sbjct: 361 YYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEELSPLWHDRLKLLFVTDGTLRIHYYSPS 420

Query: 421 FHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMLENQDVLERQLRFAIAAGI 480
           FHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMLENQDVLERQLRFAIAAGI
Sbjct: 421 FHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMLENQDVLERQLRFAIAAGI 480

Query: 481 ISQWTIGAVRGFPTESATQNLKEQVYVPSMW 511
           ISQWTIGAVRGFPTESATQNLKEQVYVPSMW
Sbjct: 481 ISQWTIGAVRGFPTESATQNLKEQVYVPSMW 511

BLAST of Cucsat.G12210 vs. ExPASy TrEMBL
Match: A0A5A7TRC7 (Fructokinase-like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001140 PE=4 SV=1)

HSP 1 Score: 976 bits (2523), Expect = 0.0
Identity = 490/511 (95.89%), Postives = 495/511 (96.87%), Query Frame = 0

Query: 1   MASFHLLPSTLPLPHVQFSCFLPFYSSNYRAKPIDLSLIFNFHHSQSLSKTLKLNPPRAS 60
           MASFHLLPSTLPLPHVQFSCFLPFYSS YRAKPIDLSLIFN  H QS SKTLKLNPPRAS
Sbjct: 1   MASFHLLPSTLPLPHVQFSCFLPFYSSTYRAKPIDLSLIFNLRHFQSSSKTLKLNPPRAS 60

Query: 61  ANDEGNGVVETPGPTRRGRKKGTASPSSSTSAQKKTKRSKEPQITDRITVANPFVHISED 120
            N EGNGVVETP PTRRGRKKGTASPSSSTSAQKKTKRSK+PQITD+ TVANPF HISED
Sbjct: 61  TNGEGNGVVETPSPTRRGRKKGTASPSSSTSAQKKTKRSKKPQITDKTTVANPFDHISED 120

Query: 121 ESIDSIADNYDDGMDIPYEDPPLICCFGAAQKEFVPSIRVHDNQMHHDKYSEWKMLQWDP 180
           ESIDSIADNYDDGMDIPYEDPPLICCFGAAQKEFVPSIRVHDNQM  DKYSEWKMLQWDP
Sbjct: 121 ESIDSIADNYDDGMDIPYEDPPLICCFGAAQKEFVPSIRVHDNQMDADKYSEWKMLQWDP 180

Query: 181 PEFARAPGGSPSNVAIAHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDLNS 240
           PEFARAPGGSPSNVAIAHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDLNS
Sbjct: 181 PEFARAPGGSPSNVAIAHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDLNS 240

Query: 241 KTACTYMKIKFEDGKLKTETVKEPAEDSLLSSELNLAVLKEARIFHFNSESLLSTAIEPT 300
           KTACTYMKIKFEDGKLK ETVKEPAEDSLLSSELNLAVLKEARIFHFNSE+LLST IEPT
Sbjct: 241 KTACTYMKIKFEDGKLKMETVKEPAEDSLLSSELNLAVLKEARIFHFNSEALLSTVIEPT 300

Query: 301 LFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWKEADIIEVSRQELEFLLDEE 360
           LFKAIQLSKKFGGLIFFDLNLPLPLWKSR+ETREYIKKAW EADIIEVSRQELEFLLDEE
Sbjct: 301 LFKAIQLSKKFGGLIFFDLNLPLPLWKSREETREYIKKAWNEADIIEVSRQELEFLLDEE 360

Query: 361 YYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEELSPLWHDRLKLLFVTDGTLRIHYYSPS 420
           YYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEE+SPLWHD LKLLFVTDGTLRIHYYSPS
Sbjct: 361 YYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEEISPLWHDHLKLLFVTDGTLRIHYYSPS 420

Query: 421 FHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMLENQDVLERQLRFAIAAGI 480
           FHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMLENQDVLERQLRFAIAAGI
Sbjct: 421 FHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMLENQDVLERQLRFAIAAGI 480

Query: 481 ISQWTIGAVRGFPTESATQNLKEQVYVPSMW 511
           ISQWTIGAVRGFPTESATQNLKEQVYVPSMW
Sbjct: 481 ISQWTIGAVRGFPTESATQNLKEQVYVPSMW 511

BLAST of Cucsat.G12210 vs. ExPASy TrEMBL
Match: A0A1S4DUM6 (fructokinase-like 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103486334 PE=4 SV=1)

HSP 1 Score: 976 bits (2523), Expect = 0.0
Identity = 490/511 (95.89%), Postives = 495/511 (96.87%), Query Frame = 0

Query: 1   MASFHLLPSTLPLPHVQFSCFLPFYSSNYRAKPIDLSLIFNFHHSQSLSKTLKLNPPRAS 60
           MASFHLLPSTLPLPHVQFSCFLPFYSS YRAKPIDLSLIFN  H QS SKTLKLNPPRAS
Sbjct: 1   MASFHLLPSTLPLPHVQFSCFLPFYSSTYRAKPIDLSLIFNLRHFQSSSKTLKLNPPRAS 60

Query: 61  ANDEGNGVVETPGPTRRGRKKGTASPSSSTSAQKKTKRSKEPQITDRITVANPFVHISED 120
            N EGNGVVETP PTRRGRKKGTASPSSSTSAQKKTKRSK+PQITD+ TVANPF HISED
Sbjct: 61  TNGEGNGVVETPSPTRRGRKKGTASPSSSTSAQKKTKRSKKPQITDKTTVANPFDHISED 120

Query: 121 ESIDSIADNYDDGMDIPYEDPPLICCFGAAQKEFVPSIRVHDNQMHHDKYSEWKMLQWDP 180
           ESIDSIADNYDDGMDIPYEDPPLICCFGAAQKEFVPSIRVHDNQM  DKYSEWKMLQWDP
Sbjct: 121 ESIDSIADNYDDGMDIPYEDPPLICCFGAAQKEFVPSIRVHDNQMDADKYSEWKMLQWDP 180

Query: 181 PEFARAPGGSPSNVAIAHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDLNS 240
           PEFARAPGGSPSNVAIAHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDLNS
Sbjct: 181 PEFARAPGGSPSNVAIAHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDLNS 240

Query: 241 KTACTYMKIKFEDGKLKTETVKEPAEDSLLSSELNLAVLKEARIFHFNSESLLSTAIEPT 300
           KTACTYMKIKFEDGKLK ETVKEPAEDSLLSSELNLAVLKEARIFHFNSE+LLST IEPT
Sbjct: 241 KTACTYMKIKFEDGKLKMETVKEPAEDSLLSSELNLAVLKEARIFHFNSEALLSTVIEPT 300

Query: 301 LFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWKEADIIEVSRQELEFLLDEE 360
           LFKAIQLSKKFGGLIFFDLNLPLPLWKSR+ETREYIKKAW EADIIEVSRQELEFLLDEE
Sbjct: 301 LFKAIQLSKKFGGLIFFDLNLPLPLWKSREETREYIKKAWNEADIIEVSRQELEFLLDEE 360

Query: 361 YYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEELSPLWHDRLKLLFVTDGTLRIHYYSPS 420
           YYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEE+SPLWHD LKLLFVTDGTLRIHYYSPS
Sbjct: 361 YYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEEISPLWHDHLKLLFVTDGTLRIHYYSPS 420

Query: 421 FHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMLENQDVLERQLRFAIAAGI 480
           FHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMLENQDVLERQLRFAIAAGI
Sbjct: 421 FHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMLENQDVLERQLRFAIAAGI 480

Query: 481 ISQWTIGAVRGFPTESATQNLKEQVYVPSMW 511
           ISQWTIGAVRGFPTESATQNLKEQVYVPSMW
Sbjct: 481 ISQWTIGAVRGFPTESATQNLKEQVYVPSMW 511

BLAST of Cucsat.G12210 vs. ExPASy TrEMBL
Match: A0A6J1CW73 (fructokinase-like 1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111015134 PE=4 SV=1)

HSP 1 Score: 858 bits (2217), Expect = 1.36e-311
Identity = 435/517 (84.14%), Postives = 463/517 (89.56%), Query Frame = 0

Query: 1   MASFHLLPSTLPLPHVQFSCFLPF-----YSSNYRAKPIDLSLIFNFHHSQSLSKTLKLN 60
           MASF LLP+T+ LPH+ FS FL       Y +     P+D  LIFN  + Q+ SK+ +LN
Sbjct: 1   MASFQLLPATISLPHLHFSRFLSNPSHCRYQTTELLNPLDSFLIFNSRYVQNSSKSPRLN 60

Query: 61  PPRASANDEGNGVVETPGPTRRGRKKG-TASPSSSTSAQKKTKRSKEPQITDRITVANPF 120
           P RAS  D+GNGVVET  PTRRGRKKG T S SSST+ QKKTKRSK+  + D  T  NPF
Sbjct: 61  PARASVGDDGNGVVETASPTRRGRKKGMTKSSSSSTTTQKKTKRSKKTLVEDETTEGNPF 120

Query: 121 VHISEDESIDSIADNYDDGMDIPYEDPPLICCFGAAQKEFVPSIRVHDNQMHHDKYSEWK 180
             +SEDE IDSIA+ YDDGMD PYEDPPLICCFGAAQKEFVPS+RVHDNQMH DKYSEWK
Sbjct: 121 EQVSEDEPIDSIAEKYDDGMDFPYEDPPLICCFGAAQKEFVPSVRVHDNQMHADKYSEWK 180

Query: 181 MLQWDPPEFARAPGGSPSNVAIAHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAV 240
           MLQWDPPEFARAPGG+PSNVAI+HVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAV
Sbjct: 181 MLQWDPPEFARAPGGAPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAV 240

Query: 241 KFDLNSKTACTYMKIKFEDGKLKTETVKEPAEDSLLSSELNLAVLKEARIFHFNSESLLS 300
           KFDLNS+TACTYMKIKFEDGKLK ETVKE AEDSLLSSELNL+VLKEARIFHFNSE+L+S
Sbjct: 241 KFDLNSRTACTYMKIKFEDGKLKMETVKESAEDSLLSSELNLSVLKEARIFHFNSEALMS 300

Query: 301 TAIEPTLFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWKEADIIEVSRQELE 360
           TA+E TLFKAIQLSKKFGGLIFFDLNL LPLW+SRDETREYIKKAW EADIIEVSRQELE
Sbjct: 301 TAMESTLFKAIQLSKKFGGLIFFDLNLLLPLWRSRDETREYIKKAWNEADIIEVSRQELE 360

Query: 361 FLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEELSPLWHDRLKLLFVTDGTLRI 420
           FLLDEEYYEKKRNYRPQYYAQTIEQTKNRRD+YHYTPEE+SPLWHDRL+LLFVTDGT+RI
Sbjct: 361 FLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYTPEEISPLWHDRLRLLFVTDGTMRI 420

Query: 421 HYYSPSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMLENQDVLERQLRF 480
           HYYSPSFHGV IGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEM ENQD LERQLRF
Sbjct: 421 HYYSPSFHGVVIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFENQDXLERQLRF 480

Query: 481 AIAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 511
           AIAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW
Sbjct: 481 AIAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 517

BLAST of Cucsat.G12210 vs. ExPASy TrEMBL
Match: A0A6J1FBN1 (fructokinase-like 1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111442595 PE=4 SV=1)

HSP 1 Score: 830 bits (2144), Expect = 1.01e-300
Identity = 427/513 (83.24%), Postives = 456/513 (88.89%), Query Frame = 0

Query: 1   MASFHLLPSTLPLPHVQFSCFLPFYSSNYRAKPIDLSLIFNFHHSQSLSKTLKLNPPRAS 60
           MASF LLP+T  LPH+QFS FL FY S +R K    +LIFN  + QS  K+LKL+PP AS
Sbjct: 1   MASFQLLPATFSLPHLQFSRFLHFYPSIFREK----TLIFNSRYLQSSCKSLKLSPPGAS 60

Query: 61  ANDEGNGVVETPGPTRRGRKKGTASPSSSTSAQKKTKRSKEPQITDRITVANPF--VHIS 120
            N +GN    TP PTRRGRKKGT SPSSS      TKRS++  I D  T  N F  + + 
Sbjct: 61  -NADGNEDTGTPTPTRRGRKKGTKSPSSS------TKRSRKTLIEDEPTQVNLFDQMSVD 120

Query: 121 EDESIDSIADNYDDGMDIPYEDPPLICCFGAAQKEFVPSIRVHDNQMHHDKYSEWKMLQW 180
           EDE+IDSIA+NYDDGMD PYEDPPLICCFGAAQKEFVPSIRVHDNQMH DKYSEWKMLQW
Sbjct: 121 EDEAIDSIAENYDDGMDFPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSEWKMLQW 180

Query: 181 DPPEFARAPGGSPSNVAIAHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDL 240
           DPPEFARAPGGSPSNVAI+HVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTR VKFDL
Sbjct: 181 DPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRGVKFDL 240

Query: 241 NSKTACTYMKIKFEDGKLKTETVKEPAEDSLLSSELNLAVLKEARIFHFNSESLLSTAIE 300
           NS+TACTYMKIKF+DGKLK ETVKE AEDSL+SSELNLAVLKEARIFHFNSE+LLST +E
Sbjct: 241 NSRTACTYMKIKFDDGKLKMETVKESAEDSLVSSELNLAVLKEARIFHFNSEALLSTTME 300

Query: 301 PTLFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWKEADIIEVSRQELEFLLD 360
            TLFKAIQLSKKFGGLIFFDLNL LPLW+SRD+TR+YI+KAW +ADIIEVSRQELEFLLD
Sbjct: 301 ATLFKAIQLSKKFGGLIFFDLNLSLPLWRSRDKTRKYIEKAWNQADIIEVSRQELEFLLD 360

Query: 361 EEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEELSPLWHDRLKLLFVTDGTLRIHYYS 420
           EEYYEKKRNYRPQYYAQTIEQTKNRRDHYHY+PEE+SPLWHD LKLL VTDGTLRIHYYS
Sbjct: 361 EEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYSPEEISPLWHDHLKLLLVTDGTLRIHYYS 420

Query: 421 PSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMLENQDVLERQLRFAIAA 480
           PSFHGV IGTEDVLITPFTCDRTGSGDA+VAGIMRKLTTFPEM E+QDVLERQLRFAIAA
Sbjct: 421 PSFHGVVIGTEDVLITPFTCDRTGSGDALVAGIMRKLTTFPEMFESQDVLERQLRFAIAA 480

Query: 481 GIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 511
           GIISQWTIGAVRGFPTESATQNLKEQVYVPSMW
Sbjct: 481 GIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 502

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M3942.2e-18970.33Fructokinase-like 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FLN1 PE=1 ... [more]
F4I0K21.6e-8641.65Fructokinase-like 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FLN2 PE=1 ... [more]
Q428965.0e-4835.80Fructokinase-2 OS=Solanum lycopersicum OX=4081 GN=FRK2 PE=2 SV=2[more]
Q7XJ811.9e-4735.80Fructokinase-2 OS=Solanum habrochaites OX=62890 GN=FRK2 PE=2 SV=1[more]
Q9M1B95.5e-4735.47Probable fructokinase-4 OS=Arabidopsis thaliana OX=3702 GN=At3g59480 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_031737955.10.0100.00fructokinase-like 1, chloroplastic [Cucumis sativus] >KGN58805.1 hypothetical pr... [more]
XP_016899692.10.095.89PREDICTED: fructokinase-like 1, chloroplastic [Cucumis melo] >KAA0044141.1 fruct... [more]
XP_038906230.10.091.59fructokinase-like 1, chloroplastic [Benincasa hispida][more]
XP_022145754.12.81e-31184.14fructokinase-like 1, chloroplastic [Momordica charantia][more]
KAG7027918.14.42e-30283.43Fructokinase-like 1, chloroplastic, partial [Cucurbita argyrosperma subsp. argyr... [more]
Match NameE-valueIdentityDescription
A0A0A0LA030.0100.00PfkB domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G732620 PE=4 S... [more]
A0A5A7TRC70.095.89Fructokinase-like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G... [more]
A0A1S4DUM60.095.89fructokinase-like 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103486334 PE=4 ... [more]
A0A6J1CW731.36e-31184.14fructokinase-like 1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC11101513... [more]
A0A6J1FBN11.01e-30083.24fructokinase-like 1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111442595... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011611Carbohydrate kinase PfkBPFAMPF00294PfkBcoord: 170..482
e-value: 5.0E-31
score: 108.1
IPR029056Ribokinase-likeGENE3D3.40.1190.20coord: 144..506
e-value: 2.9E-56
score: 193.1
IPR029056Ribokinase-likeSUPERFAMILY53613Ribokinase-likecoord: 178..495
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 53..105
NoneNo IPR availablePANTHERPTHR43085:SF10FRUCTOKINASE-LIKE 1, CHLOROPLASTICcoord: 100..509
NoneNo IPR availablePANTHERPTHR43085HEXOKINASE FAMILY MEMBERcoord: 100..509
NoneNo IPR availableCDDcd01167bac_FRKcoord: 143..489
e-value: 6.83259E-104
score: 311.107
IPR002173Carbohydrate/purine kinase, PfkB, conserved sitePROSITEPS00583PFKB_KINASES_1coord: 188..212

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G12210.T1Cucsat.G12210.T1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009658 chloroplast organization
biological_process GO:0016310 phosphorylation
biological_process GO:0042793 plastid transcription
cellular_component GO:0042644 chloroplast nucleoid
molecular_function GO:0016301 kinase activity
molecular_function GO:0016773 phosphotransferase activity, alcohol group as acceptor
molecular_function GO:0043621 protein self-association