Cucsat.G10844 (gene) Cucumber (B10) v3

Overview
NameCucsat.G10844
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionExopolygalacturonase-like
Locationctg1681: 847538 .. 853348 (-)
RNA-Seq ExpressionCucsat.G10844
SyntenyCucsat.G10844
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GCCATTAGGACAATATATAAGTAAATAAATAAAGTATAGAAGGCGTAGTAGTGATGGGATTGGCATTACTACCTTTCTTTTTATTGTTGGCATCTACTATTGAAGCCCAATCCCTTTTTGATGTTACAAGATATGGTGCCAAACCTAATACAGATATTACTCAGGCAAGTATATATGCAAAACTGATGAATTTTTTTTTTTTAATTTATTAGGAAGAAGACTTTAATTTGATGATAGAACTAAACTTTTGCAGGCCTTAGCAAATGCATGGAAGGAAGCATGTATGTCCACAACTCCTAGTAAATTGTTGATTCCAAAAGGAGTATACCAATTAAGTCAATCTTATCTTAAAGGACCTTGCAAGAGTGTTCCTATTCAAGTCCAAGTCGACGGAACACTAAAAGCTCCTCTTCATCCAAATGGAGATGGGTTGGTTCTTTTTGCATACATTGATCAACTAATGTTATCAGGCACGGGAGTTTTTGATGGTCAAGGAAAAGCAGGTTGGGATAAGAATGATTGCCACAAAAAGAAGATATGTACCAAACTTCCCATGGTAAATTGTTTATATATTATTTCAAACCTTTTGCCTCATTTTATATACAATTTAAATCTACATAATTTCAATTACATATGGGTCGTTTTAAATTGAGAGTTTTTTTTTTTTTTTTAAATTCTGATCCCTTATTTGTTTAAATCCATCAGAATTTGAAGTTCAGTTTCATCACCAATTCAATTGTGAAGGACATAACTTCCTTGGACAGTAAAAATTTCCACATCAATCTTTTGGGTTGCAAGAATGTTACTTTTCAACACGTGACAATCAGTGCACCAGAGAATAGTCCCAACACGGATGGAATTCACATAAGTAGCTCTGAACAGATAAACATTCTCGATTCAAAAATCTCAACTGGAGATGATTGTGTGTCTGTTGGAGATAGCAACAAGCAAGTAACTATTACTAATGTAACTTGCGGACCAGGACATGGCATTAGCGTAGGAAGCTTGGGCAAGTACACCAAGGAAAAAGACGTGGTAGGAGTTACTGTGAAAGCATGCAAATTAATCAATACTACCAACGGTGTCAGAATCAAAACGTGGCCAGATTCTGCCGGTGCATTCATAGCCTCTGACATGCACTTTGAAGATATTGAGATGCAAAACGTGAGCAACCCTGTCATTATAGACCAAGAATACTGTCCGTGGAATCAATGCAACAGAAAGGTATATATTGTATTGAAATTATACTTTATTTTGTTTATTAGTTAAACTTATTGAACTAAACAAATACACTTTTGGGACCATTAATATTTCAGGTTCCATCCAAGATTAAGATCAGCAAAGTGAGTTTCAAAAATATTAGAGGCACTTCTGCTACTCCTATCGCAGTGAAGCTTGTTTGCAGCAAAAGTATACCATGTGAAGGTGTAGAAGTCGCTGATATTAACCTCACTTATAGTGGAATACGAGGACCGATTTTGTCTGAATGTGCAAACGTGCAGCCTCTCATTACTGGAAAGCAAAGCCCTCAAATATGTGCTAAGCCTGCTCCTGCTGGTGCTCCATCAACAGACTAATCTTTTCAACCAATGCCACACAAAGTCTGCAAAACCATATGTGAAAGTTTATCACATATCTATATTTAATTCACAACATTATTTTGTATTCATTTTTAACATTCCATAGGTTTGATCCATTCTTTGATGATTCATGAAGCTGATCATGTACCTTTTTTCTTCCTTCCATTTTCTTCTGGGGAATGAAATATATAATGAAAATAATTCATACAATTTTAACTATTCTATCCCTTTAGACGTAGACCTACGTGAGTCGTGTTATAACATTCAATTCTACGAATGTTTTGATAACTCTTAACAACCTCAAATGTGTTATCAAACGTTGTTGAGTTTTGGAATTTTTAAAATTAATTTCTTATGTATTTAGCAAAAGCACTGATATAATTAATTTTTACATAGTTGAAATTAATATTCAATCAATCAAAAATCATAATTTAATTAATCAAGCTAGTACTATAATAAAATCTCTAAGCATCCAATAATATCATCGGAATGTATTAAAACATAATTGAATGATCATCGTTAAGTTCAAAATCTCAAAAATATGTTGAAATAGGTAAAATAATCCTTCGTACCCAAGCTAGAGTATAATCAAATTTGAAATGTAAAAAAAAATCACAAAATGATATTGCTTATTGAATTAATTAATTGTTTTTGTCAACTTTTGTAGTTTTGTTACTTGTGAAACATAGGCTTTGAAATATGCATGGATCAATGACGCATGTGCATCTGTTCGTCCAAGTGCGATGGTTCTTCTAAGGAGAATATTTAAAGTAAGAGAAGGAGAATTTAAGGCCCTTGTAATAGCCTATTGAATTGCGATGGAACATTGTCGGCTCCGAAACACTTTCATGAAGATAGTTGAATTACTTTGCATATGTTGATAGAATGAGACTATCAAATGGAGTTTTTGATGGCCAAGGAAAGGCATGTTCGGAAAAGAATGATTGCCATTAACGTATAAATTTTTCTAACCTCCGTATTGTAAGTTCTATACCTTTTGTTTTCTCTTCCATTAACCCCATTCGTCTTTGTTGATTCTGAAAGGGTTCGTTAGATTGTATTCTCTATTATTTTCATTTTTACAATTTGGTAAATGTGTTGTTAGTACCATATGATGATTAAGAAATAAAATATTGAACTAAATTTAACCATATAAAATTGAGGCAAAAGAGCTAACTTATTTTTAAAAAATATATATCAAGACTAAATATTTAGAGAAAACTCATATTTTCATCCCTCCCTAAAAGTTTGTGTGCATTATTGCTTTCTCACCGTATCTCTTACACGACTTATTGATCATATAAAATGATCAAAGTTTAAGATTTGACTTCATTACCAATTCAATTGTGAGGAGAATAACATCATTCGATAGCAAAAACTTCCATGTCAACATCCTAGGGTGAAAGAACCTCACATTCCAAGGTGTTAACATAATTGCACCAGAAGATCGTCCGAACACAGATGGAATACACATTGATCGATCAATTGGGATCTCAATCTTAAAATCTCGAATAACAACAAGAGTCAATTGCATCTCACTTGGAGATGGAAGCAAACAAATAAAAGTCACAAATGTGATATGTGGGCCATGTCATGGAATAAGCATAGGAAAGTCTTGAAAAATAAACAAATGAAGAACCTATATATCGAAAGTGTCATAGTAAAGAACTGCACAATAATGAACATCACTAATGGTGTTAGGATCAAAACATGGTACACCTCTCATGTTGCTGGTATTGCCATCAACAAGAATTTTTCAGACATTACAATGGTCAATGTTAGCAACCAAATTCTCATAGACCAAGAGTATTGTCCTTGGAATCATAGCAATCATCAGGCATTGATCACATTCTTGAAATTTTGATTTTAAAAGTTTTCATGCATATTGTCAACTTCATGGATAAACTTTAATTTTCACTCTCATTTTTCAGATTTTGTCAAAGATTAAGTTTAATAAAGTGAGCTTCGAGAATATTAGAGGAAGTTCTGCAACTCCAGTGGAAATCAAACTTATTTGTAGTAGCAACCTACCATGTGAAGAAGTAAAACTTATGTTTCATGCTTTTTTTTTCTAATATAATCTAAATTTCGACTCACTCATCCTTGATTATCAACGAGAATTTAATATCACATAATTGTTATGTTGTAGATTTTTATATTGTCGATTGAAACATATATAGATCTATTTTTGTTCAACTTGTTTTAAGCCACTTGTGTTGCACACTGTGAGGTTTGAACATGCGTGGTCTCAAAGAGGAATTTAAGGAATGAATTTTATTGGGATTTAGAGAATTAGAAGTACTTCAAAATTTCAAGGGATTATAACTAATTTTTGACAAATATAAACCATGGTTATTTAACTTCAACCATTCATCGATCTTTGCCAAATATAGTAAATAAAATTTGGAAGAGAATTTTTCAAACACTATTGATCTTCTTCTCCTATATAAAGAGGGCTGAATGAAAGTGCATATTCAAACCCATTCTTTTCAAGTTAGCCATTAGGACAATATATAAGTAAATAAATAAAGTATAGAAGGCGTAGTAGTGATGGGATTGGCATTACTACCTTTCTTTTTATTGTTGGCATCTACTATTGAAGCCCAATCCCTTTTTGATGTTACAAGATATGGTGCCAAACCTAATACAGATATTACTCAGGCAAGTATATATGCAAAATTGATGAACTTTTTTTTTAATTTATTAGGAAGAAGACTTTAATTTGATGATAGAACTAAACTTTTGCAGGCCTTAGCAAATGCATGGAAGGAAGCATGTATGTCCACAACTCCTAGTAAATTGTTGATTCCAAAAGGAGTATACCAATTAAGTCAATCTTATCTTAAAGGACCTTGCAAGAGTGTTCCTATTCAAGTCCAAGTCGACGGAACACTAAAAGCTCCTCTCCATCCAAATGGAGATGGGTTGGTTCTTTTTGCATACATTGATCAACTAATGTTATCAGGCACGGGAGTTTTTGATGGTCAAGGAAAAGCAGGTTGGGATAAGAATGATTGCCACAAAAAGAAGATATGTACCAAACTTCCCATGGTAAATTGTTTATATATTATTTCAAACCTTTTGCCTCATTTTATATACAATTTAAATCTACATAATTTCAATTACATATGCGTCGTTTTAAATTGAGAGTTTTTTTTTTTTTTTTTTTTATTCTGATCCCTTATTCGTTTAAATCCATCAGAATTTGAAGTTCAGTTTCATCACCAATTCAATTGTGAAGGACATAACTTCCTTGGACAGTAAAAATTTCCACATCAATCTTTTGGGTTGCAAGAATGTTACTTTTCAACACGTGACAATCAGTGCACCAGAGAATAGTCCCAACACGGATGGAATTCACATAAGTAGCTCTGAACAGATAAACATTCTCGATTCAAAAATCTCAACTGGAGATGATTGTGTGTCTGTTGGAGATAGCAACAAGCAAGTAACTATTACTAATGTAACTTGCGGACCAGGACATGGCATTAGCGTAGGAAGCTTGGGCAAGTACACCAAGGAAAAAGACGTGGTAGGAGTTACTGTGAAAGCATGCAAATTAATCAATACTACCAACGGTGTCAGAATCAAAACATGGCCAGATTCTGCCGGTGCATTCATAGCCTCTGACATGCACTTTGAAGATATTGAGATGCAAAACGTGAGCAACCCTGTCATTATAGACCAAGAATACTGTCCGTGGAATCAATGCAACAGAAAGGTATATATTGTATTGAAATTATACTTTATTTTGTTTATTAGTTAAACTTATTGAACTAAACAAATACACTTTTGGGACCATTAATATTTCAGGTTCCATCCAAGATTAAGATCAGCAAAGTGAGTTTCAAAAATATTAGAGGCACTTCTGCTACTCCTATCGCAGTGAAGCTTGTTTGCAGCAAAAGTATACCATGTGAAGGTGTAGAAGTCGCTGATATTAACCTCACTTATAGTGGAATACGAGGACCGATTTTGTCTGAATGTGCAAACGTGCAGCCTCTCATTACTGGAAAGCAAAGCCCTCAAATATGTGCTAAGCCTGCTCCTGCTGGTGCTCCATCAACAGACTAATCTTTTCAACCAATGCCACACAAAGTCTGCAAAACCATATGTGAAAGTTTATCACATATCTATATTTAATTCACAACTTTATTCTGTATTCATTTTTAACATTCCATAGGTTTGATCCATTCTTTGATGATTCATGAAGCTGATCATGTACCTTTTTTCTTCCTTCCATTTTCTTCTAGGGAATGAAATATATAATGAAAATAAATCATACAATTTGAACTATTCTATCCCTTTAGATGTAGACCTTCGTGAGTCGTGTTATAACATTCAATTCTACGAATGTTTTTATACTCTTAACAACCTGAAATGTGTTATCAGATTCTCTTGAGTTTTGGAAT

Coding sequence (CDS)

ATGGGATTGGCATTACTACCTTTCTTTTTATTGTTGGCATCTACTATTGAAGCCCAATCCCTTTTTGATGTTACAAGATATGGTGCCAAACCTAATACAGATATTACTCAGGCCTTAGCAAATGCATGGAAGGAAGCATGTATGTCCACAACTCCTAGTAAATTGTTGATTCCAAAAGGAGTATACCAATTAAGTCAATCTTATCTTAAAGGACCTTGCAAGAGTGTTCCTATTCAAGTCCAAGTCGACGGAACACTAAAAGCTCCTCTTCATCCAAATGGAGATGGGTTGGTTCTTTTTGCATACATTGATCAACTAATGTTATCAGGCACGGGAGTTTTTGATGGTCAAGGAAAAGCAGGTTGGGATAAGAATGATTGCCACAAAAAGAAGATATGTACCAAACTTCCCATGAATTTGAAGTTCAGTTTCATCACCAATTCAATTGTGAAGGACATAACTTCCTTGGACAGTAAAAATTTCCACATCAATCTTTTGGGTTGCAAGAATGTTACTTTTCAACACGTGACAATCAGTGCACCAGAGAATAGTCCCAACACGGATGGAATTCACATAAGTAGCTCTGAACAGATAAACATTCTCGATTCAAAAATCTCAACTGGAGATGATTGTGTGTCTGTTGGAGATAGCAACAAGCAAGTAACTATTACTAATGTAACTTGCGGACCAGGACATGGCATTAGCGTAGGAAGCTTGGGCAAGTACACCAAGGAAAAAGACGTGGTAGGAGTTACTGTGAAAGCATGCAAATTAATCAATACTACCAACGGTGTCAGAATCAAAACATGGCCAGATTCTGCCGGTGCATTCATAGCCTCTGACATGCACTTTGAAGATATTGAGATGCAAAACGTGAGCAACCCTGTCATTATAGACCAAGAATACTGTCCGTGGAATCAATGCAACAGAAAGGTTCCATCCAAGATTAAGATCAGCAAAGTGAGTTTCAAAAATATTAGAGGCACTTCTGCTACTCCTATCGCAGTGAAGCTTGTTTGCAGCAAAAGTATACCATGTGAAGGTGTAGAAGTCGCTGATATTAACCTCACTTATAGTGGAATACGAGGACCGATTTTGTCTGAATGTGCAAACGTGCAGCCTCTCATTACTGGAAAGCAAAGCCCTCAAATATGTGCTAAGCCTGCTCCTGCTGGTGCTCCATCAACAGACTAA

Protein sequence

MGLALLPFFLLLASTIEAQSLFDVTRYGAKPNTDITQALANAWKEACMSTTPSKLLIPKGVYQLSQSYLKGPCKSVPIQVQVDGTLKAPLHPNGDGLVLFAYIDQLMLSGTGVFDGQGKAGWDKNDCHKKKICTKLPMNLKFSFITNSIVKDITSLDSKNFHINLLGCKNVTFQHVTISAPENSPNTDGIHISSSEQINILDSKISTGDDCVSVGDSNKQVTITNVTCGPGHGISVGSLGKYTKEKDVVGVTVKACKLINTTNGVRIKTWPDSAGAFIASDMHFEDIEMQNVSNPVIIDQEYCPWNQCNRKVPSKIKISKVSFKNIRGTSATPIAVKLVCSKSIPCEGVEVADINLTYSGIRGPILSECANVQPLITGKQSPQICAKPAPAGAPSTD
Homology
BLAST of Cucsat.G10844 vs. ExPASy Swiss-Prot
Match: Q05967 (Polygalacturonase OS=Nicotiana tabacum OX=4097 GN=PG1 PE=2 SV=1)

HSP 1 Score: 439.1 bits (1128), Expect = 5.3e-122
Identity = 217/389 (55.78%), Postives = 284/389 (73.01%), Query Frame = 0

Query: 10  LLLASTIEAQS-LFDVTRYGAKPNTDITQALANAWKEACMSTTPSKLLIPKGVYQLSQSY 69
           L LA   E+Q+ +FD+T+YGA  N DI++AL NA+KEAC ST+PS ++IPKG + ++Q  
Sbjct: 14  LFLAHFGESQTGVFDITKYGANSNADISEALLNAFKEACQSTSPSTIVIPKGTFTMNQVK 73

Query: 70  LKGPCKSVPIQVQVDGTLKAPLHPN----GDGLVLFAYIDQLMLSGTGVFDGQGKAGWDK 129
           L+GPCKS P+++Q+  TLKAP  P+    G+ L +   +DQ  +SG G+ DGQ  A W  
Sbjct: 74  LEGPCKS-PLELQIQATLKAPSDPSQLKVGEWLTV-NKLDQFTMSGGGILDGQAAAAW-- 133

Query: 130 NDCHKKKICTKLPMNLKFSFITNSIVKDITSLDSKNFHINLLGCKNVTFQHVTISAPENS 189
            +C + K C KLP NL F+ +TNS +KDIT+LDSK+FH+N+  CKN+TF    +SAP NS
Sbjct: 134 -ECKQSKKCNKLPNNLSFNSLTNSTIKDITTLDSKSFHVNVNQCKNLTFIRFNVSAPANS 193

Query: 190 PNTDGIHISSSEQINILDSKISTGDDCVSVGDSNKQVTITNVTCGPGHGISVGSLGKYTK 249
           PNTDGIH+S S  +NI DS  STGDDC+SVGD  +Q+ IT VTCGPGHGISVGSLG    
Sbjct: 194 PNTDGIHVSRSSSVNITDSNFSTGDDCISVGDETEQLYITRVTCGPGHGISVGSLGGNPD 253

Query: 250 EKDVVGVTVKACKLINTTNGVRIKTWPDSAGAFIASDMHFEDIEMQNVSNPVIIDQEYCP 309
           EK VVGV V+ C   NT NGVRIKTWP S    + +D+HFEDI +QNVSNPV+IDQ YCP
Sbjct: 254 EKPVVGVFVRNCTFTNTDNGVRIKTWPASHPG-VVNDVHFEDIIVQNVSNPVVIDQVYCP 313

Query: 310 WNQCNRKVPSKIKISKVSFKNIRGTSATPIAVKLVCSKSIPCEGVEVADINLTYSGIRGP 369
           +N+CN+ +PS++KISKVSF+NI+GTS T  AV L+ SK +PCEG+EV DI++TYSG  GP
Sbjct: 314 FNKCNKDLPSQVKISKVSFQNIKGTSRTQDAVSLLRSKGVPCEGIEVGDIDITYSGKEGP 373

Query: 370 ILSECANVQPLITGKQSPQICAKPAPAGA 394
             S C N++P + GKQ+P +C   A + +
Sbjct: 374 AKSSCENIKPSLKGKQNPPVCTASAASSS 396

BLAST of Cucsat.G10844 vs. ExPASy Swiss-Prot
Match: Q40312 (Polygalacturonase OS=Medicago sativa OX=3879 PE=2 SV=1)

HSP 1 Score: 430.6 bits (1106), Expect = 1.9e-119
Identity = 213/402 (52.99%), Postives = 286/402 (71.14%), Query Frame = 0

Query: 4   ALLPFFLLLASTIEAQS-LFDVTRYGAKPNTDITQALANAWKEACMSTTPSKLLIPKGVY 63
           A++  FL +A    AQS + D++++G KPN+DI QAL +AW EAC STT +K++IP G Y
Sbjct: 6   AIIVSFLFIADFCAAQSGVLDISKFGGKPNSDIGQALTSAWNEACASTTAAKIVIPAGTY 65

Query: 64  QLSQSYLKGPCKSVPIQVQVDGTLKAPLHPN----GDGLVLFAYIDQLMLSGTGVFDGQG 123
           QL+   LKGPCK+ PI++QVDGT++AP  P+     +    F Y+D L LSG GVFDGQG
Sbjct: 66  QLNGIELKGPCKA-PIELQVDGTIQAPADPSVIKGTEQWFKFLYMDHLTLSGKGVFDGQG 125

Query: 124 KAGWDK----NDCHKKKICTKLPMNLKFSFITNSIVKDITSLDSKNFHINLLGCKNVTFQ 183
              + K    +    K   +K+ MN  F+F+ NSIV+ +TS DSKNFH+ + GCKN+TF 
Sbjct: 126 ATVYKKAAPASAWSGKNSNSKVFMNFGFNFVNNSIVRGVTSKDSKNFHVMVFGCKNITFD 185

Query: 184 HVTISAPENSPNTDGIHISSSEQINILDSKISTGDDCVSVGDSNKQVTITNVTCGPGHGI 243
             TI+AP +SPNTDGIH+  S  + IL++ I TGDDCVS+GD +KQ+T+  V CGPGHG+
Sbjct: 186 GFTITAPGDSPNTDGIHMGKSTDVKILNTNIGTGDDCVSIGDGSKQITVQGVNCGPGHGL 245

Query: 244 SVGSLGKYTKEKDVVGVTVKACKLINTTNGVRIKTWPDSAGAFIASDMHFEDIEMQNVSN 303
           SVGSLGK+T E++V G+TVK C L  T NGVRIKTWPD+ G    SD+HFEDI M NV N
Sbjct: 246 SVGSLGKFTTEENVEGITVKNCTLTATDNGVRIKTWPDAPGTITVSDIHFEDITMTNVKN 305

Query: 304 PVIIDQEYCPWNQCNRKVPSKIKISKVSFKNIRGTSATPIAVKLVCSKSIPCEGVEVADI 363
           PVIIDQEY PWNQC++K PSKIK+SK+SFKN++GTS T   V L+CS ++PC+GVE+ ++
Sbjct: 306 PVIIDQEYYPWNQCSKKNPSKIKLSKISFKNVKGTSGTAEGVVLICSSAVPCDGVELNNV 365

Query: 364 NLTYSGIRGPILSECANVQPLITGKQSPQICAKPAPAGAPST 397
           +L ++G   P  ++C NV+PL+TG  +  +C  P    A +T
Sbjct: 366 DLKFNG--APTTAKCTNVKPLVTG--TAPVCQAPGAPAASTT 402

BLAST of Cucsat.G10844 vs. ExPASy Swiss-Prot
Match: P24548 (Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1)

HSP 1 Score: 429.9 bits (1104), Expect = 3.2e-119
Identity = 210/364 (57.69%), Postives = 266/364 (73.08%), Query Frame = 0

Query: 34  DITQALANAWKEACMSTTPSKLLIPKGVYQLSQSYLKGPCKSVPIQVQVDGTLKAPLHPN 93
           D TQAL  AWKEAC S +PS +L+PKG + +    L+GPCKS  I +Q+ GTLKAP  P+
Sbjct: 1   DSTQALTTAWKEACASASPSTILVPKGNFAVGLITLEGPCKS-SIGLQLQGTLKAPADPS 60

Query: 94  ---GDGLVLFAYIDQLMLSGTGVFDGQGKAGWDKNDCHKK-KICTKLPMNLKFSFITNSI 153
              G G +    ID L + G GVFDGQGK+ W +NDCHK   IC  L MNL+   +TNSI
Sbjct: 61  KIKGLGWINLNKIDLLTIFGGGVFDGQGKSAWVQNDCHKNGPICKTLSMNLRLYAVTNSI 120

Query: 154 VKDITSLDSKNFHINLLGCKNVTFQHVTISAPENSPNTDGIHISSSEQINILDSKISTGD 213
           ++D+T+LDSKNFH+N++GCKN+TF+   ISA E S NTDGIHI  S+ +NI++++I TGD
Sbjct: 121 LRDVTTLDSKNFHVNVIGCKNLTFERFKISAAETSINTDGIHIGRSDGVNIINTEIKTGD 180

Query: 214 DCVSVGDSNKQVTITNVTCGPGHGISVGSLGKYTKEKDVVGVTVKACKLINTTNGVRIKT 273
           DC+S+GD +K + ITN+TCGPGHGISVGSLG+Y  E+ VVG+ VK C +  + NGVRIKT
Sbjct: 181 DCISLGDGSKNINITNITCGPGHGISVGSLGRYKNEESVVGIYVKNCTITGSQNGVRIKT 240

Query: 274 WPDSAGAFIASDMHFEDIEMQNVSNPVIIDQEYCPWNQCNRKVPSKIKISKVSFKNIRGT 333
           WP S     AS+MHF+DI M +V  P++IDQ YCP+NQC  +VPS +K+SK+SFKNI+GT
Sbjct: 241 WPKSEPG-EASEMHFQDITMNSVGTPILIDQGYCPYNQCTAEVPSSVKLSKISFKNIKGT 300

Query: 334 SATPIAVKLVCSKSIPCEGVEVADINLTYSGIRGPILSECANVQPLITGKQSPQICAKPA 393
           S T  AVKLVCSKS PC GVE+ADI+LTYSG  GP  S C N++P I GKQ P IC+  A
Sbjct: 301 STTKEAVKLVCSKSFPCNGVELADIDLTYSGKGGPATSVCENIKPTIKGKQIPAICSGSA 360

BLAST of Cucsat.G10844 vs. ExPASy Swiss-Prot
Match: Q6H9K0 (Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 SV=1)

HSP 1 Score: 416.8 bits (1070), Expect = 2.8e-115
Identity = 196/371 (52.83%), Postives = 263/371 (70.89%), Query Frame = 0

Query: 20  SLFDVTRYGAKPNTDITQALANAWKEACMSTTPSKLLIPKGVYQLSQSYLKGPCKSVPIQ 79
           S+F+V  YGAK   DI+QA+  AWK AC S  PS +LIPKG Y + +  ++GPCK   I 
Sbjct: 8   SVFNVNDYGAKGAGDISQAVMKAWKAACASQGPSTVLIPKGNYNMGEVAMQGPCKGSKIG 67

Query: 80  VQVDGTLKAPLHPN---GDGLVLFAYIDQLMLSGTGVFDGQGKAGWDKNDCHKKKICTKL 139
            Q+DG +KAP  P+    DG V F  ID L +SGTG  DGQG+  W KN+C K   C   
Sbjct: 68  FQIDGVVKAPADPSKFKSDGWVSFYRIDGLTVSGTGTLDGQGQTAWAKNNCDKNPNCKHA 127

Query: 140 PMNLKFSFITNSIVKDITSLDSKNFHINLLGCKNVTFQHVTISAPENSPNTDGIHISSSE 199
            MNL+F F+ +++V+DITSL+SK FHIN+L C+++TFQHVT++AP  S NTDGIH+  S+
Sbjct: 128 AMNLRFDFLKHAMVRDITSLNSKMFHINVLECEDITFQHVTVTAPGTSINTDGIHVGISK 187

Query: 200 QINILDSKISTGDDCVSVGDSNKQVTITNVTCGPGHGISVGSLGKYTKEKDVVGVTVKAC 259
            + I ++KI+TGDDC+S+G  ++ VTIT V CGPGHGIS+GSLG+Y  EK+V G+TVK C
Sbjct: 188 GVTITNTKIATGDDCISIGPGSQNVTITQVNCGPGHGISIGSLGRYNNEKEVRGITVKGC 247

Query: 260 KLINTTNGVRIKTWPDSAGAFIASDMHFEDIEMQNVSNPVIIDQEYCPWNQCNRKVPSKI 319
               T NGVR+KTWP+S     A+D+ F+D+ M NV NPVI+DQEYCP+ QC+R+ PS+I
Sbjct: 248 TFSGTMNGVRVKTWPNSPPG-AATDLTFQDLTMNNVQNPVILDQEYCPYGQCSRQAPSRI 307

Query: 320 KISKVSFKNIRGTSATPIAVKLVCSKSIPCEGVEVADINLTYSGIRGPILSECANVQPLI 379
           K+S ++F NIRGTS   +AV + CS  +PC  +++ +INL+Y G  GP  S C+NV+P  
Sbjct: 308 KLSNINFNNIRGTSTGKVAVVIACSHGMPCSNMKIGEINLSYRGAGGPATSTCSNVKPTF 367

Query: 380 TGKQSPQI-CA 387
           +GKQ P I CA
Sbjct: 368 SGKQVPAIKCA 377

BLAST of Cucsat.G10844 vs. ExPASy Swiss-Prot
Match: Q39786 (Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1)

HSP 1 Score: 412.1 bits (1058), Expect = 6.9e-114
Identity = 191/397 (48.11%), Postives = 277/397 (69.77%), Query Frame = 0

Query: 5   LLPFFLLLASTIEAQSLFDVTRYGAKPN--TDITQALANAWKEACMSTTPSKLLIPKGVY 64
           +L    + AS +++ +   V ++GAK +  TD+++   +AWKEAC S TPS ++IPKG Y
Sbjct: 13  VLLLLFISASKVQSDAFDVVAKFGAKADGKTDLSKPFLDAWKEACASVTPSTVVIPKGTY 72

Query: 65  QLSQSYLKGPCKSVPIQVQVDGTLKAPLHPNG---DGLVLFAYIDQLMLSGTGVFDGQGK 124
            LS+  L+GPCK+ PI++ V GT++AP  P+       V F  ++   + G G+FDGQG 
Sbjct: 73  LLSKVNLEGPCKA-PIEINVQGTIQAPADPSAFKDPNWVRFYSVENFKMFGGGIFDGQGS 132

Query: 125 AGWDKNDCHKKKICTKLPMNLKFSFITNSIVKDITSLDSKNFHINLLGCKNVTFQHVTIS 184
             ++KN C  ++  +KLP+N++F F+TN++++DITS DSK FHIN+  CKN+T + + I 
Sbjct: 133 IAYEKNTCENREFRSKLPVNIRFDFLTNALIQDITSKDSKLFHINVFACKNITLERLKIE 192

Query: 185 APENSPNTDGIHISSSEQINILDSKISTGDDCVSVGDSNKQVTITNVTCGPGHGISVGSL 244
           AP+ SPNTDGIH+  SE +NI+ S I TGDDC+S+GD  K + I  +TCGPGHGIS+GSL
Sbjct: 193 APDESPNTDGIHMGKSEGVNIIASDIKTGDDCISIGDGTKNMVIKEITCGPGHGISIGSL 252

Query: 245 GKYTKEKDVVGVTVKACKLINTTNGVRIKTWPDSAGAFIASDMHFEDIEMQNVSNPVIID 304
           GK+  E+ V G+ +  C + NT+NG RIKTWP   G  + S++HFEDI M NVS+P++ID
Sbjct: 253 GKFQNEEPVEGIKISNCTITNTSNGARIKTWPGEHGGAV-SEIHFEDITMNNVSSPILID 312

Query: 305 QEYCPWNQCNRKVPSKIKISKVSFKNIRGTSATPIAVKLVCSKSIPCEGVEVADINLTYS 364
           Q+YCPWN+C +   SK+K+S +SFKNIRGTSA P A+K +CS S PC+ VE+ADI++ ++
Sbjct: 313 QQYCPWNKCKKNEESKVKLSNISFKNIRGTSALPEAIKFICSGSSPCQNVELADIDIKHN 372

Query: 365 GIRGPILSECANVQPLITGKQSPQICAKPAPAGAPST 397
           G   P  S+C NV+P+ +GK +P  C+ P P    +T
Sbjct: 373 GAE-PATSQCLNVKPITSGKLNPIPCSGPVPKTPSAT 406

BLAST of Cucsat.G10844 vs. NCBI nr
Match: XP_031744814.1 (exopolygalacturonase-like [Cucumis sativus] >XP_031744815.1 LOW QUALITY PROTEIN: exopolygalacturonase [Cucumis sativus] >XP_031744816.1 exopolygalacturonase-like [Cucumis sativus] >XP_031744818.1 exopolygalacturonase-like [Cucumis sativus] >KAE8645716.1 hypothetical protein Csa_020252 [Cucumis sativus])

HSP 1 Score: 800 bits (2067), Expect = 2.26e-292
Identity = 397/397 (100.00%), Postives = 397/397 (100.00%), Query Frame = 0

Query: 1   MGLALLPFFLLLASTIEAQSLFDVTRYGAKPNTDITQALANAWKEACMSTTPSKLLIPKG 60
           MGLALLPFFLLLASTIEAQSLFDVTRYGAKPNTDITQALANAWKEACMSTTPSKLLIPKG
Sbjct: 1   MGLALLPFFLLLASTIEAQSLFDVTRYGAKPNTDITQALANAWKEACMSTTPSKLLIPKG 60

Query: 61  VYQLSQSYLKGPCKSVPIQVQVDGTLKAPLHPNGDGLVLFAYIDQLMLSGTGVFDGQGKA 120
           VYQLSQSYLKGPCKSVPIQVQVDGTLKAPLHPNGDGLVLFAYIDQLMLSGTGVFDGQGKA
Sbjct: 61  VYQLSQSYLKGPCKSVPIQVQVDGTLKAPLHPNGDGLVLFAYIDQLMLSGTGVFDGQGKA 120

Query: 121 GWDKNDCHKKKICTKLPMNLKFSFITNSIVKDITSLDSKNFHINLLGCKNVTFQHVTISA 180
           GWDKNDCHKKKICTKLPMNLKFSFITNSIVKDITSLDSKNFHINLLGCKNVTFQHVTISA
Sbjct: 121 GWDKNDCHKKKICTKLPMNLKFSFITNSIVKDITSLDSKNFHINLLGCKNVTFQHVTISA 180

Query: 181 PENSPNTDGIHISSSEQINILDSKISTGDDCVSVGDSNKQVTITNVTCGPGHGISVGSLG 240
           PENSPNTDGIHISSSEQINILDSKISTGDDCVSVGDSNKQVTITNVTCGPGHGISVGSLG
Sbjct: 181 PENSPNTDGIHISSSEQINILDSKISTGDDCVSVGDSNKQVTITNVTCGPGHGISVGSLG 240

Query: 241 KYTKEKDVVGVTVKACKLINTTNGVRIKTWPDSAGAFIASDMHFEDIEMQNVSNPVIIDQ 300
           KYTKEKDVVGVTVKACKLINTTNGVRIKTWPDSAGAFIASDMHFEDIEMQNVSNPVIIDQ
Sbjct: 241 KYTKEKDVVGVTVKACKLINTTNGVRIKTWPDSAGAFIASDMHFEDIEMQNVSNPVIIDQ 300

Query: 301 EYCPWNQCNRKVPSKIKISKVSFKNIRGTSATPIAVKLVCSKSIPCEGVEVADINLTYSG 360
           EYCPWNQCNRKVPSKIKISKVSFKNIRGTSATPIAVKLVCSKSIPCEGVEVADINLTYSG
Sbjct: 301 EYCPWNQCNRKVPSKIKISKVSFKNIRGTSATPIAVKLVCSKSIPCEGVEVADINLTYSG 360

Query: 361 IRGPILSECANVQPLITGKQSPQICAKPAPAGAPSTD 397
           IRGPILSECANVQPLITGKQSPQICAKPAPAGAPSTD
Sbjct: 361 IRGPILSECANVQPLITGKQSPQICAKPAPAGAPSTD 397

BLAST of Cucsat.G10844 vs. NCBI nr
Match: XP_031744817.1 (exopolygalacturonase-like [Cucumis sativus] >KAE8645715.1 hypothetical protein Csa_020330 [Cucumis sativus])

HSP 1 Score: 799 bits (2063), Expect = 9.20e-292
Identity = 396/397 (99.75%), Postives = 397/397 (100.00%), Query Frame = 0

Query: 1   MGLALLPFFLLLASTIEAQSLFDVTRYGAKPNTDITQALANAWKEACMSTTPSKLLIPKG 60
           MGLALLPFFLLLASTIEAQSLFDVTRYGAKPNTDITQALANAWKEACMSTTPSKLLIPKG
Sbjct: 1   MGLALLPFFLLLASTIEAQSLFDVTRYGAKPNTDITQALANAWKEACMSTTPSKLLIPKG 60

Query: 61  VYQLSQSYLKGPCKSVPIQVQVDGTLKAPLHPNGDGLVLFAYIDQLMLSGTGVFDGQGKA 120
           VYQLSQSYLKGPCKSVPIQVQVDGTLKAPLHPNGDGLVLFAYIDQLMLSGTGVFDGQGKA
Sbjct: 61  VYQLSQSYLKGPCKSVPIQVQVDGTLKAPLHPNGDGLVLFAYIDQLMLSGTGVFDGQGKA 120

Query: 121 GWDKNDCHKKKICTKLPMNLKFSFITNSIVKDITSLDSKNFHINLLGCKNVTFQHVTISA 180
           GWDKNDCHKKKICTKLPMNLKFSFITNSIVKDITSLDSKNFHINLLGCKNVTFQHVTISA
Sbjct: 121 GWDKNDCHKKKICTKLPMNLKFSFITNSIVKDITSLDSKNFHINLLGCKNVTFQHVTISA 180

Query: 181 PENSPNTDGIHISSSEQINILDSKISTGDDCVSVGDSNKQVTITNVTCGPGHGISVGSLG 240
           PENSPNTDGIHISSSEQINILDSKISTGDDCVSVGDSNKQVTITNVTCGPGHGISVGSLG
Sbjct: 181 PENSPNTDGIHISSSEQINILDSKISTGDDCVSVGDSNKQVTITNVTCGPGHGISVGSLG 240

Query: 241 KYTKEKDVVGVTVKACKLINTTNGVRIKTWPDSAGAFIASDMHFEDIEMQNVSNPVIIDQ 300
           KYTKEKDVVGV+VKACKLINTTNGVRIKTWPDSAGAFIASDMHFEDIEMQNVSNPVIIDQ
Sbjct: 241 KYTKEKDVVGVSVKACKLINTTNGVRIKTWPDSAGAFIASDMHFEDIEMQNVSNPVIIDQ 300

Query: 301 EYCPWNQCNRKVPSKIKISKVSFKNIRGTSATPIAVKLVCSKSIPCEGVEVADINLTYSG 360
           EYCPWNQCNRKVPSKIKISKVSFKNIRGTSATPIAVKLVCSKSIPCEGVEVADINLTYSG
Sbjct: 301 EYCPWNQCNRKVPSKIKISKVSFKNIRGTSATPIAVKLVCSKSIPCEGVEVADINLTYSG 360

Query: 361 IRGPILSECANVQPLITGKQSPQICAKPAPAGAPSTD 397
           IRGPILSECANVQPLITGKQSPQICAKPAPAGAPSTD
Sbjct: 361 IRGPILSECANVQPLITGKQSPQICAKPAPAGAPSTD 397

BLAST of Cucsat.G10844 vs. NCBI nr
Match: KAA0059167.1 (exopolygalacturonase-like [Cucumis melo var. makuwa] >KAA0059172.1 exopolygalacturonase-like [Cucumis melo var. makuwa] >TYK19347.1 exopolygalacturonase-like [Cucumis melo var. makuwa])

HSP 1 Score: 717 bits (1852), Expect = 1.48e-259
Identity = 352/399 (88.22%), Postives = 375/399 (93.98%), Query Frame = 0

Query: 1   MGLALLPFFLLL--ASTIEAQSLFDVTRYGAKPNTDITQALANAWKEACMSTTPSKLLIP 60
           MGLALLPF LLL  ASTI+ QS+FDVT YGAKPNTDITQALANAW EAC STTPS LLIP
Sbjct: 2   MGLALLPFLLLLSLASTIQGQSIFDVTTYGAKPNTDITQALANAWTEACASTTPSTLLIP 61

Query: 61  KGVYQLSQSYLKGPCKSVPIQVQVDGTLKAPLHPNGDGLVLFAYIDQLMLSGTGVFDGQG 120
           KGVYQLSQS LKGPCKSVPIQVQV+GTL+AP+H  GDGLVLF+YIDQL+LSG+GVFDGQG
Sbjct: 62  KGVYQLSQSNLKGPCKSVPIQVQVEGTLQAPIHTKGDGLVLFSYIDQLILSGSGVFDGQG 121

Query: 121 KAGWDKNDCHKKKICTKLPMNLKFSFITNSIVKDITSLDSKNFHINLLGCKNVTFQHVTI 180
           K+GW+KN+CHKKKICTKLPMNLKFSF+TNS VKDITSLDSKNFHINLLGCKNVTFQHVTI
Sbjct: 122 KSGWEKNNCHKKKICTKLPMNLKFSFVTNSTVKDITSLDSKNFHINLLGCKNVTFQHVTI 181

Query: 181 SAPENSPNTDGIHISSSEQINILDSKISTGDDCVSVGDSNKQVTITNVTCGPGHGISVGS 240
            AP+ SPNTDGIHISSSE+I ILDSKI+TGDDCVSVGDSNKQVTITNVTCGPGHGIS+GS
Sbjct: 182 IAPDESPNTDGIHISSSEEIYILDSKIATGDDCVSVGDSNKQVTITNVTCGPGHGISIGS 241

Query: 241 LGKYTKEKDVVGVTVKACKLINTTNGVRIKTWPDSAGAFIASDMHFEDIEMQNVSNPVII 300
           LGKYTKEKDVVGVTVKACK+ NTTNGVRIKTWPDSA AF+ASDMHFEDIEM+NVSNP+II
Sbjct: 242 LGKYTKEKDVVGVTVKACKIFNTTNGVRIKTWPDSAVAFMASDMHFEDIEMENVSNPIII 301

Query: 301 DQEYCPWNQCNRKVPSKIKISKVSFKNIRGTSATPIAVKLVCSKSIPCEGVEVADINLTY 360
           DQEYCPWNQCNRKVPSKIKIS VSFKNIRGTSATP+AVKLVCSKSIPCEGVEVADINLTY
Sbjct: 302 DQEYCPWNQCNRKVPSKIKISNVSFKNIRGTSATPVAVKLVCSKSIPCEGVEVADINLTY 361

Query: 361 SGIRGPILSECANVQPLITGKQSPQICAKPAPAGAPSTD 397
           SG RGPI+SECANV P ITG Q+PQICAKPA  GAPSTD
Sbjct: 362 SGNRGPIVSECANVMPTITGVQNPQICAKPATIGAPSTD 400

BLAST of Cucsat.G10844 vs. NCBI nr
Match: KAA0059162.1 (exopolygalacturonase-like [Cucumis melo var. makuwa])

HSP 1 Score: 709 bits (1831), Expect = 7.60e-251
Identity = 348/395 (88.10%), Postives = 371/395 (93.92%), Query Frame = 0

Query: 1   MGLALLPFFLLL--ASTIEAQSLFDVTRYGAKPNTDITQALANAWKEACMSTTPSKLLIP 60
           MGLALLPF LLL  ASTI+ QS+FDVT YGAKPNTDITQALANAW EAC STTPS LLIP
Sbjct: 2   MGLALLPFLLLLSLASTIQGQSIFDVTTYGAKPNTDITQALANAWTEACASTTPSTLLIP 61

Query: 61  KGVYQLSQSYLKGPCKSVPIQVQVDGTLKAPLHPNGDGLVLFAYIDQLMLSGTGVFDGQG 120
           KGVYQLSQS LKGPCKSVPIQVQV+GTL+AP+H  GDGLVLF+YIDQL+LSG+GVFDGQG
Sbjct: 62  KGVYQLSQSNLKGPCKSVPIQVQVEGTLQAPIHTKGDGLVLFSYIDQLILSGSGVFDGQG 121

Query: 121 KAGWDKNDCHKKKICTKLPMNLKFSFITNSIVKDITSLDSKNFHINLLGCKNVTFQHVTI 180
           K+GW+KN+CHKKKICTKLPMNLKFSF+TNS VKDITSLDSKNFHINLLGCKNVTFQHVTI
Sbjct: 122 KSGWEKNNCHKKKICTKLPMNLKFSFVTNSTVKDITSLDSKNFHINLLGCKNVTFQHVTI 181

Query: 181 SAPENSPNTDGIHISSSEQINILDSKISTGDDCVSVGDSNKQVTITNVTCGPGHGISVGS 240
            AP+ SPNTDGIHISSSE+I ILDSKI+TGDDCVSVGDSNKQVTITNVTCGPGHGIS+GS
Sbjct: 182 IAPDESPNTDGIHISSSEEIYILDSKIATGDDCVSVGDSNKQVTITNVTCGPGHGISIGS 241

Query: 241 LGKYTKEKDVVGVTVKACKLINTTNGVRIKTWPDSAGAFIASDMHFEDIEMQNVSNPVII 300
           LGKYTKEKDVVGVTVKACK+ NTTNGVRIKTWPDSA AF+ASDMHFEDIEM+NVSNP+II
Sbjct: 242 LGKYTKEKDVVGVTVKACKIFNTTNGVRIKTWPDSAVAFMASDMHFEDIEMENVSNPIII 301

Query: 301 DQEYCPWNQCNRKVPSKIKISKVSFKNIRGTSATPIAVKLVCSKSIPCEGVEVADINLTY 360
           DQEYCPWNQCNRKVPSKIKIS VSFKNIRGTSATP+AVKLVCSKSIPCEGVEVADINLTY
Sbjct: 302 DQEYCPWNQCNRKVPSKIKISNVSFKNIRGTSATPVAVKLVCSKSIPCEGVEVADINLTY 361

Query: 361 SGIRGPILSECANVQPLITGKQSPQICAKPAPAGA 393
           SG RGPI+SECANV P ITG Q+PQICAKPA  GA
Sbjct: 362 SGNRGPIVSECANVMPTITGVQNPQICAKPATIGA 396

BLAST of Cucsat.G10844 vs. NCBI nr
Match: TYK02527.1 (exopolygalacturonase-like [Cucumis melo var. makuwa])

HSP 1 Score: 708 bits (1828), Expect = 1.89e-250
Identity = 348/395 (88.10%), Postives = 371/395 (93.92%), Query Frame = 0

Query: 1   MGLALLPFFLLL--ASTIEAQSLFDVTRYGAKPNTDITQALANAWKEACMSTTPSKLLIP 60
           MGLALLPF LLL  ASTI+ QS+FDVT YGAKPNTDITQALANAW EAC STTPS LLIP
Sbjct: 2   MGLALLPFLLLLSLASTIQGQSIFDVTTYGAKPNTDITQALANAWTEACASTTPSTLLIP 61

Query: 61  KGVYQLSQSYLKGPCKSVPIQVQVDGTLKAPLHPNGDGLVLFAYIDQLMLSGTGVFDGQG 120
           KGVYQLSQS LKGPCKSVPIQVQV+GTL+AP+H  GDGLVLF+YIDQL+LSG+GVFDGQG
Sbjct: 62  KGVYQLSQSNLKGPCKSVPIQVQVEGTLQAPIHTKGDGLVLFSYIDQLILSGSGVFDGQG 121

Query: 121 KAGWDKNDCHKKKICTKLPMNLKFSFITNSIVKDITSLDSKNFHINLLGCKNVTFQHVTI 180
           K+GW+KN+CHKKKICTKLPMNLKFSF+TNS VKDITSLDSKNFHINLLGCKNVTFQHVTI
Sbjct: 122 KSGWEKNNCHKKKICTKLPMNLKFSFVTNSTVKDITSLDSKNFHINLLGCKNVTFQHVTI 181

Query: 181 SAPENSPNTDGIHISSSEQINILDSKISTGDDCVSVGDSNKQVTITNVTCGPGHGISVGS 240
            AP+ SPNTDGIHISSSE+I ILDSKI+TGDDCVSVGDSNKQVTITNVTCGPGHGIS+GS
Sbjct: 182 IAPDESPNTDGIHISSSEEIYILDSKIATGDDCVSVGDSNKQVTITNVTCGPGHGISIGS 241

Query: 241 LGKYTKEKDVVGVTVKACKLINTTNGVRIKTWPDSAGAFIASDMHFEDIEMQNVSNPVII 300
           LGKYTKEKDVVGVTVKACK+ NTTNGVRIKTWPDSA AF+ASDMHFEDIEM+NVSNP+II
Sbjct: 242 LGKYTKEKDVVGVTVKACKIFNTTNGVRIKTWPDSAVAFMASDMHFEDIEMENVSNPIII 301

Query: 301 DQEYCPWNQCNRKVPSKIKISKVSFKNIRGTSATPIAVKLVCSKSIPCEGVEVADINLTY 360
           DQEYCPWNQCNRKVPSKIKIS VSFKNIRGTSATP+AVKLVCSKSIPCEGVEVADINLTY
Sbjct: 302 DQEYCPWNQCNRKVPSKIKISNVSFKNIRGTSATPVAVKLVCSKSIPCEGVEVADINLTY 361

Query: 361 SGIRGPILSECANVQPLITGKQSPQICAKPAPAGA 393
           SG RGPI+SECANV P ITG Q+PQICAKPA A A
Sbjct: 362 SGNRGPIVSECANVMPTITGVQNPQICAKPALANA 396

BLAST of Cucsat.G10844 vs. ExPASy TrEMBL
Match: A0A5D3D708 (Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold295G00680 PE=3 SV=1)

HSP 1 Score: 717 bits (1852), Expect = 7.17e-260
Identity = 352/399 (88.22%), Postives = 375/399 (93.98%), Query Frame = 0

Query: 1   MGLALLPFFLLL--ASTIEAQSLFDVTRYGAKPNTDITQALANAWKEACMSTTPSKLLIP 60
           MGLALLPF LLL  ASTI+ QS+FDVT YGAKPNTDITQALANAW EAC STTPS LLIP
Sbjct: 2   MGLALLPFLLLLSLASTIQGQSIFDVTTYGAKPNTDITQALANAWTEACASTTPSTLLIP 61

Query: 61  KGVYQLSQSYLKGPCKSVPIQVQVDGTLKAPLHPNGDGLVLFAYIDQLMLSGTGVFDGQG 120
           KGVYQLSQS LKGPCKSVPIQVQV+GTL+AP+H  GDGLVLF+YIDQL+LSG+GVFDGQG
Sbjct: 62  KGVYQLSQSNLKGPCKSVPIQVQVEGTLQAPIHTKGDGLVLFSYIDQLILSGSGVFDGQG 121

Query: 121 KAGWDKNDCHKKKICTKLPMNLKFSFITNSIVKDITSLDSKNFHINLLGCKNVTFQHVTI 180
           K+GW+KN+CHKKKICTKLPMNLKFSF+TNS VKDITSLDSKNFHINLLGCKNVTFQHVTI
Sbjct: 122 KSGWEKNNCHKKKICTKLPMNLKFSFVTNSTVKDITSLDSKNFHINLLGCKNVTFQHVTI 181

Query: 181 SAPENSPNTDGIHISSSEQINILDSKISTGDDCVSVGDSNKQVTITNVTCGPGHGISVGS 240
            AP+ SPNTDGIHISSSE+I ILDSKI+TGDDCVSVGDSNKQVTITNVTCGPGHGIS+GS
Sbjct: 182 IAPDESPNTDGIHISSSEEIYILDSKIATGDDCVSVGDSNKQVTITNVTCGPGHGISIGS 241

Query: 241 LGKYTKEKDVVGVTVKACKLINTTNGVRIKTWPDSAGAFIASDMHFEDIEMQNVSNPVII 300
           LGKYTKEKDVVGVTVKACK+ NTTNGVRIKTWPDSA AF+ASDMHFEDIEM+NVSNP+II
Sbjct: 242 LGKYTKEKDVVGVTVKACKIFNTTNGVRIKTWPDSAVAFMASDMHFEDIEMENVSNPIII 301

Query: 301 DQEYCPWNQCNRKVPSKIKISKVSFKNIRGTSATPIAVKLVCSKSIPCEGVEVADINLTY 360
           DQEYCPWNQCNRKVPSKIKIS VSFKNIRGTSATP+AVKLVCSKSIPCEGVEVADINLTY
Sbjct: 302 DQEYCPWNQCNRKVPSKIKISNVSFKNIRGTSATPVAVKLVCSKSIPCEGVEVADINLTY 361

Query: 361 SGIRGPILSECANVQPLITGKQSPQICAKPAPAGAPSTD 397
           SG RGPI+SECANV P ITG Q+PQICAKPA  GAPSTD
Sbjct: 362 SGNRGPIVSECANVMPTITGVQNPQICAKPATIGAPSTD 400

BLAST of Cucsat.G10844 vs. ExPASy TrEMBL
Match: A0A5A7UTD3 (Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold430G00800 PE=3 SV=1)

HSP 1 Score: 709 bits (1831), Expect = 3.68e-251
Identity = 348/395 (88.10%), Postives = 371/395 (93.92%), Query Frame = 0

Query: 1   MGLALLPFFLLL--ASTIEAQSLFDVTRYGAKPNTDITQALANAWKEACMSTTPSKLLIP 60
           MGLALLPF LLL  ASTI+ QS+FDVT YGAKPNTDITQALANAW EAC STTPS LLIP
Sbjct: 2   MGLALLPFLLLLSLASTIQGQSIFDVTTYGAKPNTDITQALANAWTEACASTTPSTLLIP 61

Query: 61  KGVYQLSQSYLKGPCKSVPIQVQVDGTLKAPLHPNGDGLVLFAYIDQLMLSGTGVFDGQG 120
           KGVYQLSQS LKGPCKSVPIQVQV+GTL+AP+H  GDGLVLF+YIDQL+LSG+GVFDGQG
Sbjct: 62  KGVYQLSQSNLKGPCKSVPIQVQVEGTLQAPIHTKGDGLVLFSYIDQLILSGSGVFDGQG 121

Query: 121 KAGWDKNDCHKKKICTKLPMNLKFSFITNSIVKDITSLDSKNFHINLLGCKNVTFQHVTI 180
           K+GW+KN+CHKKKICTKLPMNLKFSF+TNS VKDITSLDSKNFHINLLGCKNVTFQHVTI
Sbjct: 122 KSGWEKNNCHKKKICTKLPMNLKFSFVTNSTVKDITSLDSKNFHINLLGCKNVTFQHVTI 181

Query: 181 SAPENSPNTDGIHISSSEQINILDSKISTGDDCVSVGDSNKQVTITNVTCGPGHGISVGS 240
            AP+ SPNTDGIHISSSE+I ILDSKI+TGDDCVSVGDSNKQVTITNVTCGPGHGIS+GS
Sbjct: 182 IAPDESPNTDGIHISSSEEIYILDSKIATGDDCVSVGDSNKQVTITNVTCGPGHGISIGS 241

Query: 241 LGKYTKEKDVVGVTVKACKLINTTNGVRIKTWPDSAGAFIASDMHFEDIEMQNVSNPVII 300
           LGKYTKEKDVVGVTVKACK+ NTTNGVRIKTWPDSA AF+ASDMHFEDIEM+NVSNP+II
Sbjct: 242 LGKYTKEKDVVGVTVKACKIFNTTNGVRIKTWPDSAVAFMASDMHFEDIEMENVSNPIII 301

Query: 301 DQEYCPWNQCNRKVPSKIKISKVSFKNIRGTSATPIAVKLVCSKSIPCEGVEVADINLTY 360
           DQEYCPWNQCNRKVPSKIKIS VSFKNIRGTSATP+AVKLVCSKSIPCEGVEVADINLTY
Sbjct: 302 DQEYCPWNQCNRKVPSKIKISNVSFKNIRGTSATPVAVKLVCSKSIPCEGVEVADINLTY 361

Query: 361 SGIRGPILSECANVQPLITGKQSPQICAKPAPAGA 393
           SG RGPI+SECANV P ITG Q+PQICAKPA  GA
Sbjct: 362 SGNRGPIVSECANVMPTITGVQNPQICAKPATIGA 396

BLAST of Cucsat.G10844 vs. ExPASy TrEMBL
Match: A0A5D3BSV2 (Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold201G00060 PE=3 SV=1)

HSP 1 Score: 708 bits (1828), Expect = 9.17e-251
Identity = 348/395 (88.10%), Postives = 371/395 (93.92%), Query Frame = 0

Query: 1   MGLALLPFFLLL--ASTIEAQSLFDVTRYGAKPNTDITQALANAWKEACMSTTPSKLLIP 60
           MGLALLPF LLL  ASTI+ QS+FDVT YGAKPNTDITQALANAW EAC STTPS LLIP
Sbjct: 2   MGLALLPFLLLLSLASTIQGQSIFDVTTYGAKPNTDITQALANAWTEACASTTPSTLLIP 61

Query: 61  KGVYQLSQSYLKGPCKSVPIQVQVDGTLKAPLHPNGDGLVLFAYIDQLMLSGTGVFDGQG 120
           KGVYQLSQS LKGPCKSVPIQVQV+GTL+AP+H  GDGLVLF+YIDQL+LSG+GVFDGQG
Sbjct: 62  KGVYQLSQSNLKGPCKSVPIQVQVEGTLQAPIHTKGDGLVLFSYIDQLILSGSGVFDGQG 121

Query: 121 KAGWDKNDCHKKKICTKLPMNLKFSFITNSIVKDITSLDSKNFHINLLGCKNVTFQHVTI 180
           K+GW+KN+CHKKKICTKLPMNLKFSF+TNS VKDITSLDSKNFHINLLGCKNVTFQHVTI
Sbjct: 122 KSGWEKNNCHKKKICTKLPMNLKFSFVTNSTVKDITSLDSKNFHINLLGCKNVTFQHVTI 181

Query: 181 SAPENSPNTDGIHISSSEQINILDSKISTGDDCVSVGDSNKQVTITNVTCGPGHGISVGS 240
            AP+ SPNTDGIHISSSE+I ILDSKI+TGDDCVSVGDSNKQVTITNVTCGPGHGIS+GS
Sbjct: 182 IAPDESPNTDGIHISSSEEIYILDSKIATGDDCVSVGDSNKQVTITNVTCGPGHGISIGS 241

Query: 241 LGKYTKEKDVVGVTVKACKLINTTNGVRIKTWPDSAGAFIASDMHFEDIEMQNVSNPVII 300
           LGKYTKEKDVVGVTVKACK+ NTTNGVRIKTWPDSA AF+ASDMHFEDIEM+NVSNP+II
Sbjct: 242 LGKYTKEKDVVGVTVKACKIFNTTNGVRIKTWPDSAVAFMASDMHFEDIEMENVSNPIII 301

Query: 301 DQEYCPWNQCNRKVPSKIKISKVSFKNIRGTSATPIAVKLVCSKSIPCEGVEVADINLTY 360
           DQEYCPWNQCNRKVPSKIKIS VSFKNIRGTSATP+AVKLVCSKSIPCEGVEVADINLTY
Sbjct: 302 DQEYCPWNQCNRKVPSKIKISNVSFKNIRGTSATPVAVKLVCSKSIPCEGVEVADINLTY 361

Query: 361 SGIRGPILSECANVQPLITGKQSPQICAKPAPAGA 393
           SG RGPI+SECANV P ITG Q+PQICAKPA A A
Sbjct: 362 SGNRGPIVSECANVMPTITGVQNPQICAKPALANA 396

BLAST of Cucsat.G10844 vs. ExPASy TrEMBL
Match: A0A6J1C2U8 (exopolygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111007858 PE=3 SV=1)

HSP 1 Score: 645 bits (1664), Expect = 4.83e-231
Identity = 310/385 (80.52%), Postives = 346/385 (89.87%), Query Frame = 0

Query: 13  ASTIEAQSLFDVTRYGAKPNTDITQALANAWKEACMSTTPSKLLIPKGVYQLSQSYLKGP 72
           A T  A  +FDV +YGAKPN DITQA+A+AWKEAC ST+PSKLLIPKG YQL  +  +GP
Sbjct: 29  APTGGAGGVFDVAKYGAKPNADITQAVASAWKEACASTSPSKLLIPKGSYQLKGASFRGP 88

Query: 73  CKSVPIQVQVDGTLKAPLHPNGDGLVLFAYIDQLMLSGTGVFDGQGKAGWDKNDCHKKKI 132
           CKS  I +QVDGTL+AP HP G+G ++  YIDQL LSGTGVFDGQGKAGW+KNDCH KKI
Sbjct: 89  CKSA-IDIQVDGTLQAPPHPKGEGWIMLEYIDQLTLSGTGVFDGQGKAGWEKNDCHLKKI 148

Query: 133 CTKLPMNLKFSFITNSIVKDITSLDSKNFHINLLGCKNVTFQHVTISAPENSPNTDGIHI 192
           CTKLPM+LKF+F+TNS+VKDITSLDSKNFHINLLGCKNVTFQHVTISAP++SPNTDGIHI
Sbjct: 149 CTKLPMSLKFNFVTNSVVKDITSLDSKNFHINLLGCKNVTFQHVTISAPDHSPNTDGIHI 208

Query: 193 SSSEQINILDSKISTGDDCVSVGDSNKQVTITNVTCGPGHGISVGSLGKYTKEKDVVGVT 252
           SSSE INIL++ I TGDDC+SVGDSN Q+TI++VTCGPGHGIS+GSLGKY+KEKDV+GV 
Sbjct: 209 SSSENINILNTNIGTGDDCISVGDSNSQITISDVTCGPGHGISIGSLGKYSKEKDVIGVF 268

Query: 253 VKACKLINTTNGVRIKTWPDSAGAFIASDMHFEDIEMQNVSNPVIIDQEYCPWNQCNRKV 312
           VK CKL +TTNGVRIKTWPDSAGAFIASDMHFEDIEM NVSNPVIIDQEYCPWNQCNRKV
Sbjct: 269 VKKCKLTSTTNGVRIKTWPDSAGAFIASDMHFEDIEMVNVSNPVIIDQEYCPWNQCNRKV 328

Query: 313 PSKIKISKVSFKNIRGTSATPIAVKLVCSKSIPCEGVEVADINLTYSGIRGPILSECANV 372
           PSKIKISKVSFKNIRGTSATP+AVKLVCS S PCEGVEVADINLTY+G +GPI SECANV
Sbjct: 329 PSKIKISKVSFKNIRGTSATPVAVKLVCSSSNPCEGVEVADINLTYNGKQGPIKSECANV 388

Query: 373 QPLITGKQSPQICAKPAPAGAPSTD 397
           +P ITG+Q+P+ICAK AP  APSTD
Sbjct: 389 KPTITGQQNPKICAKVAPPNAPSTD 412

BLAST of Cucsat.G10844 vs. ExPASy TrEMBL
Match: A0A5A7TRZ0 (Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold280G00600 PE=3 SV=1)

HSP 1 Score: 639 bits (1649), Expect = 6.91e-229
Identity = 309/396 (78.03%), Postives = 353/396 (89.14%), Query Frame = 0

Query: 5   LLPFFLLLASTIEAQS---LFDVTRYGAKPNTDITQALANAWKEACMSTTPSKLLIPKGV 64
           LLP  LLL S  +AQS   +FD+T+YGAKPN D+T ALA+AWKEAC STTPSK+LIPKG 
Sbjct: 10  LLPVLLLLVSNSKAQSSGGVFDITKYGAKPNADVTAALASAWKEACASTTPSKVLIPKGS 69

Query: 65  YQLSQSYLKGPCKSVPIQVQVDGTLKAPLHPNGDGLVLFAYIDQLMLSGTGVFDGQGKAG 124
           Y LSQS LKGPCKS  I++Q++GTL+AP  P GDGL+L  Y+DQL +SGTGV DGQGKAG
Sbjct: 70  YGLSQSNLKGPCKSA-IELQIEGTLQAPADPKGDGLLLLEYVDQLTVSGTGVLDGQGKAG 129

Query: 125 WDKNDCHKKKICTKLPMNLKFSFITNSIVKDITSLDSKNFHINLLGCKNVTFQHVTISAP 184
           W+KNDCH KK CTKLPMNLK +F+TNSIVKDITSLDSKN+HINLLGCKN+TF HVTI+AP
Sbjct: 130 WEKNDCHLKKECTKLPMNLKLNFVTNSIVKDITSLDSKNYHINLLGCKNLTFDHVTITAP 189

Query: 185 ENSPNTDGIHISSSEQINILDSKISTGDDCVSVGDSNKQVTITNVTCGPGHGISVGSLGK 244
            NSPNTDGIH+SSSEQINIL++ I+TGDDC+SVGD+NKQV I++VTCGPGHGIS+GSLGK
Sbjct: 190 GNSPNTDGIHVSSSEQINILNTNIATGDDCISVGDTNKQVVISDVTCGPGHGISIGSLGK 249

Query: 245 YTKEKDVVGVTVKACKLINTTNGVRIKTWPDSAGAFIASDMHFEDIEMQNVSNPVIIDQE 304
           Y+KEK+VVGVTVK CKL +TTNGVRIKTWPDSA AF ASDMHFEDIEM NVSNPVIIDQE
Sbjct: 250 YSKEKEVVGVTVKKCKLTDTTNGVRIKTWPDSAVAFPASDMHFEDIEMVNVSNPVIIDQE 309

Query: 305 YCPWNQCNRKVPSKIKISKVSFKNIRGTSATPIAVKLVCSKSIPCEGVEVADINLTYSGI 364
           YCPWNQCNRKVPSKIKIS VSFKNIRGTSATP+AVK+VCS+S PCEGVEVADI+LTYSG 
Sbjct: 310 YCPWNQCNRKVPSKIKISNVSFKNIRGTSATPVAVKIVCSQSNPCEGVEVADIDLTYSGS 369

Query: 365 RGPILSECANVQPLITGKQSPQICAKPAPAGAPSTD 397
            GPI S+CANV+P+I+GKQ+P IC +PAPAG PS+D
Sbjct: 370 EGPIKSQCANVKPVISGKQNPPICGEPAPAGGPSSD 404

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q059675.3e-12255.78Polygalacturonase OS=Nicotiana tabacum OX=4097 GN=PG1 PE=2 SV=1[more]
Q403121.9e-11952.99Polygalacturonase OS=Medicago sativa OX=3879 PE=2 SV=1[more]
P245483.2e-11957.69Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1[more]
Q6H9K02.8e-11552.83Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 S... [more]
Q397866.9e-11448.11Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_031744814.12.26e-292100.00exopolygalacturonase-like [Cucumis sativus] >XP_031744815.1 LOW QUALITY PROTEIN:... [more]
XP_031744817.19.20e-29299.75exopolygalacturonase-like [Cucumis sativus] >KAE8645715.1 hypothetical protein C... [more]
KAA0059167.11.48e-25988.22exopolygalacturonase-like [Cucumis melo var. makuwa] >KAA0059172.1 exopolygalact... [more]
KAA0059162.17.60e-25188.10exopolygalacturonase-like [Cucumis melo var. makuwa][more]
TYK02527.11.89e-25088.10exopolygalacturonase-like [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
A0A5D3D7087.17e-26088.22Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A5A7UTD33.68e-25188.10Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo... [more]
A0A5D3BSV29.17e-25188.10Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A6J1C2U84.83e-23180.52exopolygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111007858 PE=3 SV... [more]
A0A5A7TRZ06.91e-22978.03Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 195..216
e-value: 1500.0
score: 5.9
coord: 248..269
e-value: 46.0
score: 12.9
coord: 218..238
e-value: 3400.0
score: 3.4
coord: 168..194
e-value: 3.7
score: 16.5
coord: 279..300
e-value: 8400.0
score: 0.6
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 47..373
e-value: 1.6E-100
score: 336.3
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 225..238
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 12..390
e-value: 1.2E-105
score: 355.8
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 8..389
NoneNo IPR availablePANTHERPTHR31375:SF248POLYGALACTURONASEcoord: 8..389
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 8..386

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G10844.T2Cucsat.G10844.T2mRNA
Cucsat.G10844.T1Cucsat.G10844.T1mRNA
Cucsat.G10844.T3Cucsat.G10844.T3mRNA
Cucsat.G10844.T5Cucsat.G10844.T5mRNA
Cucsat.G10844.T4Cucsat.G10844.T4mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity