Cucsat.G10004 (gene) Cucumber (B10) v3

Overview
NameCucsat.G10004
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Descriptioncleavage and polyadenylation specificity factor subunit 3-I
Locationctg1673: 3143687 .. 3147525 (+)
RNA-Seq ExpressionCucsat.G10004
SyntenyCucsat.G10004
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CCATTCCCTCGTTTCTCAATTACTCGCGACTTTCCCAAAATGCACTCCTCGTCTAAAATCTCTCCCCTTCTTCTCTTCTCTTCTCTCCCGCTGAATTTCTCCGTCTAAAACCCAACAAACAACAATTCAATTACACAGTTTTTTTTTATCTTTTGAATTCTAGGGTTTAGCTCTCTTTCTTCCTTCATCTCTCAACACTGTAAGTTCTCTTTCTACACATTTTCCCTCTTCTACTTACTACTTCACTCTCTTTCCCCCTCTTCTTCAACCTTTTTAGATCTGTTTCTGTTTAACAAATACTTATTATGTTTACATATTTTGGAATTGTAAGATTTAATTCTGTCTTCCCTTTTGCTTTTGATATCTTGCAGCTGGAGAGATTTTGGAAAAATGGCTTCGGTTGGACAGCCACCCTCCCTTAAGAAACGGGATGCATCCTCGACCAGAGAGGAGGATCAGCTTATTATAACTCCTCTTGGGGCTGGCAATGAAGTGGGTCGTTCCTGTGTATATATGTCTTACAAAAGCAAAATTGTTTTGGTATGGTTTGAACTCATTATTATTTCTCATTTACTATCTGGGTATAAATAACATATGGAGGTTTAATTGTGTTGGTGGTAATTTTTCTTTTCTCCTCTTGTTTTTCTTGGATTACAGTTTGATTGTGGAATTCACCCTGCTTACTCGGGTATGGCTGCTTTGCCTTACTTTGATGAGATTGACCCTTCAACGATTGATGTCCTGCTCATTACCCAGTATGTATTTCTTTCCTTCTTTCTTTCTAAAATATTTAGATAATGCAGCTCTTAACTTGGACATTTTGTGTTTTATAGCTCTTTTTTACTTATGCAAAATTAGTGATCACATTCTTTCGGAACTCGTGTTCTACATATTGTTAAGACTCTTGCCTCCTTACTAAAATCATGGAAAATGCAAATATTCCCGAGGAGCAGTTTTTCAGTGTCTTTTTTAATCTGATCATGGCACGAGATTCTAATTTTTCCAGCTCTCTTTATTTTCTGCCTCTTATTGTTTTTTGTGTTCTTTTAACCAAGATAATTTCGTGCACACTTATTTCACAGCTTTCATTTGGACCATGCTGCATCACTCCCTTATTTCTTGGAGAAGGTACTTTCTATTTGACTTTCAATGTGATTGTTGCTCGAGTACAGAAACTAATCTAGCATTAATTTGATAGACTACATTCAAAGGACGAGTCTTTATGACTTATGCTACTAAAGCGATCTACAAGTTGTTGCTCTCGGACTTTGTGAAAGTGAGCAAAGTTTCAGTTGAAGATATGTTGTATGATGAGCAGGACATAAATCGTTCCATGGACAAGATTGAGGTAAATTATTTCTCACTGTGGACATCTCTCGAGTCTTGACTTGCTGTTTCATCCATCGTGATAATTAATTTTCAGTGATTCTACCAAAATCGTACCTTCTATTAAAAATCTGCTTGACGTTTTATTTAGTAACAGTTTATCAAATGTACATTCCACTCGCACTATCTGTCATTCGTCTGCCTCTGCCCTTATCTCCTCCTGCTTTAGTTCTCTGTGAATTTTTTCTGTTAAAATTTTGAAATTCAGTTCTTATAATTTTAGAATAAAATATGATTGAACTACAAGTGGTCTTTTGTTGTATTCATTGAAATTTGCTGTTTTATGAAGTTTAGTTTCTTTTGAATGCTACATTTTTTATAATCTTATTCAGGATGATAAAATAGCAAAGATATCTGTATGATAATCATTTAAGTCCTCGAGATAATAATGATAATGAACCAGCAACCAGCTATGGTGGAATGCGGAATCATTATATTGGTTATTTTGGTGTCAACTTGCATGGGTTTCATATCCTTGCACCCTGATTTGTATAGTTGAAAATGGATATCGGTTATTCATTCTCTTATTTAATAATAATGCACAGTAATCGCAGGTCATTGATTTCCATCAAACGGTAGAAGTTAATGGTATTCGGTTTTGGTGTTACACTGCTGGTCATGTGCTTGGTGCTGCCATGTTTATGGTGGATATCGCTGGTGTCCGAGTTCTCTACACTGGAGACTACTCACGTGAAGAAGATCGGCATCTTCGGGCTGCCGAGATGCCTCAGTTTTCTCCTGATGTTTGCATAATTGAATCCACATATGGTGTCCAGCTCCATCAACCTCGACATATTCGAGAGAAGCGTTTTACCGATGTTGTTCATTCAACCATTTCTCAAGGTGGTCGCGTACTGATTCCAGCTTTTGCCCTTGGACGTGCCCAGGAGCTCCTCCTTATTCTTGATGAATATTGGGCAAACCATCCTGAGCTCCATAATATTCCCATATACTATGCTTCTCCTCTGGCAAAAAGATGTTTGACTGTATATGAGACATATACGCTCTCCATGAATGATAGGATCCAAAATGCCAAATCAAACCCCTTCCGATTCAAGTACATATCCCCACTAAAGAGCATTGAAGTTTTCAAGGATGTTGGCCCATCAGTGGTGATGGCCAGCCCTGGTGGACTTCAAAGCGGTTTGTCGCGTCAGCTCTTTGACATGTGGTGTTCTGACAAGAAAAATTCATGTGTGCTTCCTGGTTATGTCGTTGAAGGGACACTGGCTAAGACTATAATCAATGAGCCAAAGGAAGTCACCCTCATGAGTGGACTCATGGCTCCTCTCAACATGCAGGTTCATTACATTTCTTTCTCTGCCCATGCTGACTTTGCGCAGACGAGCGCATTCTTGGAAGAGCTCATGCCACCCAACATAATTCTCGTCCATGGAGAAGCTAATGAGATGGGGAGGCTGAAACAGAAGCTTATGTCCCAATTTGCTGATCGGAATACAAAGATTCTCACTCCGAAGAATTGTCAGTCTGTTGAGATGTATTTCAACTCTCAGAAAATGGCAAAAACTATTGGAAAATTGGCTGAGAAGACTCCAGACGTTGGCGAAACTGTCAGCGGTTTACTAGTGAAGAAAGGATTTGCATATCAAATAATGGCACCAGAGGATCTACACATTTTCTCTCAGCTATCAACTGCAAACATCAACCAACGCATTACAATTCCATACTCCAACGCCTTTAATGTGATCGTACGTAGGCTCAAACAGGTATATGAGAGTGTAGAATCTTCAACCGATGATGAATCCGGCGTTCCAATGATTCGTGTGCATGATCGAGTAACCGTGAAGCAGGAATCAGAGAAACATGTCTCACTTCACTGGACATCCGACCCTTTAAGCGACATGGTATCAGATTCCGTGGTAGCACTCATTCTCAACATCAACCGCGAGGTTCCGAAGGTCATAGTGGAGTCTGAGGCTGTAAAAACAGAAGAAGAGAACGTGAAGAAAGCAGAGAAGGTAATTCATGCCCTGCTTGTTTCACTGTTCGGCGATGTGAAGTTAGGAGAGAATGGGAAGCTGGTGATTAACGTAGATGGGAATATAGCAGAGGTTGATAAACAAAGTGGTGAAGTAGAAAGTGATAATGAAGCTCTCAAGGAAAGAGTTAAAACAGCATTTCAAAGAATTCAATGCGCTGTGAACCCAATTCCTCTTTCATCATCATCTTAGCTTTTAGCTTTAGCTTTAGCTTTCTGTATTTGGCATGTTCAAGTAGACTAATTTGTATTATTTCCTCTGCCTACTTTCAAAAATAGGGAAAAAAAAACTGTATTTTACAAGTTTTTTTTACTTCATTTTTGAATTGCTTATTTTGTTTGTCCACTTGGATTTTGGTAATTGTTTAAAGAAAGTGAGAAAAAGAGAAGTGTAAACGTCGTATGCCATATCATATTTGAATGGTGTTTAGCAAATGACAGCAAAGGACAGTTTCGTGAAAGTTTATTTTTGTCATCATCGGAATTGCTTCATTTCAACCTTCCTTTTACTCTTCCTTTGAGATTTTATC

Coding sequence (CDS)

ATGGCTTCGGTTGGACAGCCACCCTCCCTTAAGAAACGGGATGCATCCTCGACCAGAGAGGAGGATCAGCTTATTATAACTCCTCTTGGGGCTGGCAATGAAGTGGGTCGTTCCTGTGTATATATGTCTTACAAAAGCAAAATTGTTTTGTTTGATTGTGGAATTCACCCTGCTTACTCGGGTATGGCTGCTTTGCCTTACTTTGATGAGATTGACCCTTCAACGATTGATGTCCTGCTCATTACCCACTTTCATTTGGACCATGCTGCATCACTCCCTTATTTCTTGGAGAAGACTACATTCAAAGGACGAGTCTTTATGACTTATGCTACTAAAGCGATCTACAAGTTGTTGCTCTCGGACTTTGTGAAAGTGAGCAAAGTTTCAGTTGAAGATATGTTGTATGATGAGCAGGACATAAATCGTTCCATGGACAAGATTGAGGTCATTGATTTCCATCAAACGGTAGAAGTTAATGGTATTCGGTTTTGGTGTTACACTGCTGGTCATGTGCTTGGTGCTGCCATGTTTATGGTGGATATCGCTGGTGTCCGAGTTCTCTACACTGGAGACTACTCACGTGAAGAAGATCGGCATCTTCGGGCTGCCGAGATGCCTCAGTTTTCTCCTGATGTTTGCATAATTGAATCCACATATGGTGTCCAGCTCCATCAACCTCGACATATTCGAGAGAAGCGTTTTACCGATGTTGTTCATTCAACCATTTCTCAAGGTGGTCGCGTACTGATTCCAGCTTTTGCCCTTGGACGTGCCCAGGAGCTCCTCCTTATTCTTGATGAATATTGGGCAAACCATCCTGAGCTCCATAATATTCCCATATACTATGCTTCTCCTCTGGCAAAAAGATGTTTGACTGTATATGAGACATATACGCTCTCCATGAATGATAGGATCCAAAATGCCAAATCAAACCCCTTCCGATTCAAGTACATATCCCCACTAAAGAGCATTGAAGTTTTCAAGGATGTTGGCCCATCAGTGGTGATGGCCAGCCCTGGTGGACTTCAAAGCGGTTTGTCGCGTCAGCTCTTTGACATGTGGTGTTCTGACAAGAAAAATTCATGTGTGCTTCCTGGTTATGTCGTTGAAGGGACACTGGCTAAGACTATAATCAATGAGCCAAAGGAAGTCACCCTCATGAGTGGACTCATGGCTCCTCTCAACATGCAGGTTCATTACATTTCTTTCTCTGCCCATGCTGACTTTGCGCAGACGAGCGCATTCTTGGAAGAGCTCATGCCACCCAACATAATTCTCGTCCATGGAGAAGCTAATGAGATGGGGAGGCTGAAACAGAAGCTTATGTCCCAATTTGCTGATCGGAATACAAAGATTCTCACTCCGAAGAATTGTCAGTCTGTTGAGATGTATTTCAACTCTCAGAAAATGGCAAAAACTATTGGAAAATTGGCTGAGAAGACTCCAGACGTTGGCGAAACTGTCAGCGGTTTACTAGTGAAGAAAGGATTTGCATATCAAATAATGGCACCAGAGGATCTACACATTTTCTCTCAGCTATCAACTGCAAACATCAACCAACGCATTACAATTCCATACTCCAACGCCTTTAATGTGATCGTACGTAGGCTCAAACAGGTATATGAGAGTGTAGAATCTTCAACCGATGATGAATCCGGCGTTCCAATGATTCGTGTGCATGATCGAGTAACCGTGAAGCAGGAATCAGAGAAACATGTCTCACTTCACTGGACATCCGACCCTTTAAGCGACATGGTATCAGATTCCGTGGTAGCACTCATTCTCAACATCAACCGCGAGGTTCCGAAGGTCATAGTGGAGTCTGAGGCTGTAAAAACAGAAGAAGAGAACGTGAAGAAAGCAGAGAAGGTAATTCATGCCCTGCTTGTTTCACTGTTCGGCGATGTGAAGTTAGGAGAGAATGGGAAGCTGGTGATTAACGTAGATGGGAATATAGCAGAGGTTGATAAACAAAGTGGTGAAGTAGAAAGTGATAATGAAGCTCTCAAGGAAAGAGTTAAAACAGCATTTCAAAGAATTCAATGCGCTGTGAACCCAATTCCTCTTTCATCATCATCTTAG

Protein sequence

MASVGQPPSLKKRDASSTREEDQLIITPLGAGNEVGRSCVYMSYKSKIVLFDCGIHPAYSGMAALPYFDEIDPSTIDVLLITHFHLDHAASLPYFLEKTTFKGRVFMTYATKAIYKLLLSDFVKVSKVSVEDMLYDEQDINRSMDKIEVIDFHQTVEVNGIRFWCYTAGHVLGAAMFMVDIAGVRVLYTGDYSREEDRHLRAAEMPQFSPDVCIIESTYGVQLHQPRHIREKRFTDVVHSTISQGGRVLIPAFALGRAQELLLILDEYWANHPELHNIPIYYASPLAKRCLTVYETYTLSMNDRIQNAKSNPFRFKYISPLKSIEVFKDVGPSVVMASPGGLQSGLSRQLFDMWCSDKKNSCVLPGYVVEGTLAKTIINEPKEVTLMSGLMAPLNMQVHYISFSAHADFAQTSAFLEELMPPNIILVHGEANEMGRLKQKLMSQFADRNTKILTPKNCQSVEMYFNSQKMAKTIGKLAEKTPDVGETVSGLLVKKGFAYQIMAPEDLHIFSQLSTANINQRITIPYSNAFNVIVRRLKQVYESVESSTDDESGVPMIRVHDRVTVKQESEKHVSLHWTSDPLSDMVSDSVVALILNINREVPKVIVESEAVKTEEENVKKAEKVIHALLVSLFGDVKLGENGKLVINVDGNIAEVDKQSGEVESDNEALKERVKTAFQRIQCAVNPIPLSSSS
Homology
BLAST of Cucsat.G10004 vs. ExPASy Swiss-Prot
Match: Q9C952 (Cleavage and polyadenylation specificity factor subunit 3-I OS=Arabidopsis thaliana OX=3702 GN=CPSF73-I PE=1 SV=1)

HSP 1 Score: 986.1 bits (2548), Expect = 1.7e-286
Identity = 481/589 (81.66%), Postives = 551/589 (93.55%), Query Frame = 0

Query: 1   MTYATKAIYKLLLSDFVKVSKVSVEDMLYDEQDINRSMDKIEVIDFHQTVEVNGIRFWCY 60
           MT+ATKAIYKLLL+D+VKVSKVSVEDML+DEQDIN+SMDKIEVIDFHQTVEVNGI+FWCY
Sbjct: 105 MTHATKAIYKLLLTDYVKVSKVSVEDMLFDEQDINKSMDKIEVIDFHQTVEVNGIKFWCY 164

Query: 61  TAGHVLGAAMFMVDIAGVRVLYTGDYSREEDRHLRAAEMPQFSPDVCIIESTYGVQLHQP 120
           TAGHVLGAAMFMVDIAGVR+LYTGDYSREEDRHLRAAE+PQFSPD+CIIEST GVQLHQ 
Sbjct: 165 TAGHVLGAAMFMVDIAGVRILYTGDYSREEDRHLRAAELPQFSPDICIIESTSGVQLHQS 224

Query: 121 RHIREKRFTDVVHSTISQGGRVLIPAFALGRAQELLLILDEYWANHPELHNIPIYYASPL 180
           RHIREKRFTDV+HST++QGGRVLIPAFALGRAQELLLILDEYWANHP+LHNIPIYYASPL
Sbjct: 225 RHIREKRFTDVIHSTVAQGGRVLIPAFALGRAQELLLILDEYWANHPDLHNIPIYYASPL 284

Query: 181 AKRCLTVYETYTLSMNDRIQN--AKSNPFRFKYISPLKSIEVFKDVGPSVVMASPGGLQS 240
           AK+C+ VY+TY LSMNDRI+N  A SNPF FK+ISPL SI+ F DVGPSVVMA+PGGLQS
Sbjct: 285 AKKCMAVYQTYILSMNDRIRNQFANSNPFVFKHISPLNSIDDFNDVGPSVVMATPGGLQS 344

Query: 241 GLSRQLFDMWCSDKKNSCVLPGYVVEGTLAKTIINEPKEVTLMSGLMAPLNMQVHYISFS 300
           GLSRQLFD WCSDKKN+C++PGY+VEGTLAKTIINEPKEVTLM+GL APLNMQVHYISFS
Sbjct: 345 GLSRQLFDSWCSDKKNACIIPGYMVEGTLAKTIINEPKEVTLMNGLTAPLNMQVHYISFS 404

Query: 301 AHADFAQTSAFLEELMPPNIILVHGEANEMGRLKQKLMSQFADRNTKILTPKNCQSVEMY 360
           AHAD+AQTS FL+ELMPPNIILVHGEANEM RLKQKL+++F D NTKI+TPKNC+SVEMY
Sbjct: 405 AHADYAQTSTFLKELMPPNIILVHGEANEMMRLKQKLLTEFPDGNTKIMTPKNCESVEMY 464

Query: 361 FNSQKMAKTIGKLAEKTPDVGETVSGLLVKKGFAYQIMAPEDLHIFSQLSTANINQRITI 420
           FNS+K+AKTIG+LAEKTPDVG+TVSG+LVKKGF YQIMAP++LH+FSQLSTA + QRITI
Sbjct: 465 FNSEKLAKTIGRLAEKTPDVGDTVSGILVKKGFTYQIMAPDELHVFSQLSTATVTQRITI 524

Query: 421 PYSNAFNVIVRRLKQVYESVESSTDDESGVPMIRVHDRVTVKQESEKHVSLHWTSDPLSD 480
           P+  AF VI  RL++++ESVE STD+ESG+P ++VH+RVTVKQESEKH+SL W+SDP+SD
Sbjct: 525 PFVGAFGVIKHRLEKIFESVEFSTDEESGLPALKVHERVTVKQESEKHISLQWSSDPISD 584

Query: 481 MVSDSVVALILNINREVPKVIVESE-AVKTEEENVKKAEKVIHALLVSLFGDVKLGENGK 540
           MVSDS+VALILNI+REVPK+++E E AVK+EEEN KK EKVI+ALLVSLFGDVKLGENGK
Sbjct: 585 MVSDSIVALILNISREVPKIVMEEEDAVKSEEENGKKVEKVIYALLVSLFGDVKLGENGK 644

Query: 541 LVINVDGNIAEVDKQSGEVESDNEALKERVKTAFQRIQCAVNPIPLSSS 587
           LVI VDGN+A++DK+SGEVES++  LKERV+ AF+RIQ AV PIPLS+S
Sbjct: 645 LVIRVDGNVAQLDKESGEVESEHSGLKERVRVAFERIQSAVKPIPLSAS 693

BLAST of Cucsat.G10004 vs. ExPASy Swiss-Prot
Match: P79101 (Cleavage and polyadenylation specificity factor subunit 3 OS=Bos taurus OX=9913 GN=CPSF3 PE=1 SV=1)

HSP 1 Score: 581.3 bits (1497), Expect = 1.3e-164
Identity = 301/595 (50.59%), Postives = 415/595 (69.75%), Query Frame = 0

Query: 1   MTYATKAIYKLLLSDFVKVSKVSVEDMLYDEQDINRSMDKIEVIDFHQTVEVNGIRFWCY 60
           MT+ATKAIY+ LLSD+VKVS +S +DMLY E D+  SMDKIE I+FH+  EV GI+FWCY
Sbjct: 95  MTHATKAIYRWLLSDYVKVSNISADDMLYTETDLEESMDKIETINFHEVKEVAGIKFWCY 154

Query: 61  TAGHVLGAAMFMVDIAGVRVLYTGDYSREEDRHLRAAEMPQFSPDVCIIESTYGVQLHQP 120
            AGHVLGAAMFM++IAGV++LYTGD+SR+EDRHL AAE+P   PD+ IIESTYG  +H+ 
Sbjct: 155 HAGHVLGAAMFMIEIAGVKLLYTGDFSRQEDRHLMAAEIPNIKPDILIIESTYGTHIHEK 214

Query: 121 RHIREKRFTDVVHSTISQGGRVLIPAFALGRAQELLLILDEYWANHPELHNIPIYYASPL 180
           R  RE RF + VH  +++GGR LIP FALGRAQELLLILDEYW NHPELH+IPIYYAS L
Sbjct: 215 REEREARFCNTVHDIVNRGGRGLIPVFALGRAQELLLILDEYWQNHPELHDIPIYYASSL 274

Query: 181 AKRCLTVYETYTLSMNDRI--QNAKSNPFRFKYISPLKSIEVFKDVGPSVVMASPGGLQS 240
           AK+C+ VY+TY  +MND+I  Q   +NPF FK+IS LKS++ F D+GPSVVMASPG +QS
Sbjct: 275 AKKCMAVYQTYVNAMNDKIRKQININNPFVFKHISNLKSMDHFDDIGPSVVMASPGMMQS 334

Query: 241 GLSRQLFDMWCSDKKNSCVLPGYVVEGTLAKTIINEPKEVTLMSGLMAPLNMQVHYISFS 300
           GLSR+LF+ WC+DK+N  ++ GY VEGTLAK I++EP+E+T MSG   PL M V YISFS
Sbjct: 335 GLSRELFESWCTDKRNGVIIAGYCVEGTLAKHIMSEPEEITTMSGQKLPLKMSVDYISFS 394

Query: 301 AHADFAQTSAFLEELMPPNIILVHGEANEMGRLKQKLMSQFADR---NTKILTPKNCQSV 360
           AH D+ QTS F+  L PP++ILVHGE NEM RLK  L+ ++ D    + ++  P+N ++V
Sbjct: 395 AHTDYQQTSEFIRALKPPHVILVHGEQNEMARLKAALIREYEDNDEVHIEVHNPRNTEAV 454

Query: 361 EMYFNSQKMAKTIGKLAEKTPDVGETVSGLLVKKGFAYQIMAPEDLHIFSQLSTANINQR 420
            + F  +K+AK +G LA+K P+ G+ VSG+LVK+ F Y I++P DL  ++ L+ + + Q 
Sbjct: 455 TLNFRGEKLAKVMGFLADKKPEQGQRVSGILVKRNFNYHILSPCDLSNYTDLAMSTVKQT 514

Query: 421 ITIPYSNAFNVIVRRLKQVYESVESSTDDESGVPMIRVHDRVTVKQESEKHVSLHWTSDP 480
             IPY+  FN++  +L+++   VE     E   P ++V   +TV QE    V L W ++P
Sbjct: 515 QAIPYTGPFNLLYYQLQKLTGDVEELEIQEK--PALKVFKNITVIQEPGM-VVLEWLANP 574

Query: 481 LSDMVSDSVVALILNI--NREVPKVIVESEAVKTEEENVKKAEKVIHALLVSLFGD--VK 540
            +DM +D+V  +IL +  N ++ K  V+  + K E   +    K +  +L  +FG+  V 
Sbjct: 575 SNDMYADTVTTVILEVQSNPKIRKGAVQKVSKKLE---MHVYSKRLEIMLQDIFGEDCVS 634

Query: 541 LGENGKLVINVDGNIAEVDKQSGEVE-----SDNEALKERVKTAFQRIQCAVNPI 582
           + +   L + VDG  A ++ ++  VE      D+E+L+E V+ A QR+  A+ P+
Sbjct: 635 VKDGSILSVTVDGKTANINLETRTVECEEGSEDDESLREMVELAAQRLYEALTPV 683

BLAST of Cucsat.G10004 vs. ExPASy Swiss-Prot
Match: Q9UKF6 (Cleavage and polyadenylation specificity factor subunit 3 OS=Homo sapiens OX=9606 GN=CPSF3 PE=1 SV=1)

HSP 1 Score: 580.9 bits (1496), Expect = 1.7e-164
Identity = 301/595 (50.59%), Postives = 416/595 (69.92%), Query Frame = 0

Query: 1   MTYATKAIYKLLLSDFVKVSKVSVEDMLYDEQDINRSMDKIEVIDFHQTVEVNGIRFWCY 60
           MT+ATKAIY+ LLSD+VKVS +S +DMLY E D+  SMDKIE I+FH+  EV GI+FWCY
Sbjct: 95  MTHATKAIYRWLLSDYVKVSNISADDMLYTETDLEESMDKIETINFHEVKEVAGIKFWCY 154

Query: 61  TAGHVLGAAMFMVDIAGVRVLYTGDYSREEDRHLRAAEMPQFSPDVCIIESTYGVQLHQP 120
            AGHVLGAAMFM++IAGV++LYTGD+SR+EDRHL AAE+P   PD+ IIESTYG  +H+ 
Sbjct: 155 HAGHVLGAAMFMIEIAGVKLLYTGDFSRQEDRHLMAAEIPNIKPDILIIESTYGTHIHEK 214

Query: 121 RHIREKRFTDVVHSTISQGGRVLIPAFALGRAQELLLILDEYWANHPELHNIPIYYASPL 180
           R  RE RF + VH  +++GGR LIP FALGRAQELLLILDEYW NHPELH+IPIYYAS L
Sbjct: 215 REEREARFCNTVHDIVNRGGRGLIPVFALGRAQELLLILDEYWQNHPELHDIPIYYASSL 274

Query: 181 AKRCLTVYETYTLSMNDRI--QNAKSNPFRFKYISPLKSIEVFKDVGPSVVMASPGGLQS 240
           AK+C+ VY+TY  +MND+I  Q   +NPF FK+IS LKS++ F D+GPSVVMASPG +QS
Sbjct: 275 AKKCMAVYQTYVNAMNDKIRKQININNPFVFKHISNLKSMDHFDDIGPSVVMASPGMMQS 334

Query: 241 GLSRQLFDMWCSDKKNSCVLPGYVVEGTLAKTIINEPKEVTLMSGLMAPLNMQVHYISFS 300
           GLSR+LF+ WC+DK+N  ++ GY VEGTLAK I++EP+E+T MSG   PL M V YISFS
Sbjct: 335 GLSRELFESWCTDKRNGVIIAGYCVEGTLAKHIMSEPEEITTMSGQKLPLKMSVDYISFS 394

Query: 301 AHADFAQTSAFLEELMPPNIILVHGEANEMGRLKQKLMSQFADR---NTKILTPKNCQSV 360
           AH D+ QTS F+  L PP++ILVHGE NEM RLK  L+ ++ D    + ++  P+N ++V
Sbjct: 395 AHTDYQQTSEFIRALKPPHVILVHGEQNEMARLKAALIREYEDNDEVHIEVHNPRNTEAV 454

Query: 361 EMYFNSQKMAKTIGKLAEKTPDVGETVSGLLVKKGFAYQIMAPEDLHIFSQLSTANINQR 420
            + F  +K+AK +G LA+K P+ G+ VSG+LVK+ F Y I++P DL  ++ L+ + + Q 
Sbjct: 455 TLNFRGEKLAKVMGFLADKKPEQGQRVSGILVKRNFNYHILSPCDLSNYTDLAMSTVKQT 514

Query: 421 ITIPYSNAFNVIVRRLKQVYESVESSTDDESGVPMIRVHDRVTVKQESEKHVSLHWTSDP 480
             IPY+  FN++  +L+++   VE     E   P ++V   +TV QE    V L W ++P
Sbjct: 515 QAIPYTGPFNLLCYQLQKLTGDVEELEIQEK--PALKVFKNITVIQEPGM-VVLEWLANP 574

Query: 481 LSDMVSDSVVALILNI--NREVPKVIVESEAVKTEEENVKKAEKVIHALLVSLFGD--VK 540
            +DM +D+V  +IL +  N ++ K  V+  + K E   +    K +  +L  +FG+  V 
Sbjct: 575 SNDMYADTVTTVILEVQSNPKIRKGAVQKVSKKLE---MHVYSKRLEIMLQDIFGEDCVS 634

Query: 541 LGENGKLVINVDGNIAEVDKQSGEVE-----SDNEALKERVKTAFQRIQCAVNPI 582
           + ++  L + VDG  A ++ ++  VE      D+E+L+E V+ A QR+  A+ P+
Sbjct: 635 VKDDSILSVTVDGKTANLNLETRTVECEEGSEDDESLREMVELAAQRLYEALTPV 683

BLAST of Cucsat.G10004 vs. ExPASy Swiss-Prot
Match: Q9QXK7 (Cleavage and polyadenylation specificity factor subunit 3 OS=Mus musculus OX=10090 GN=Cpsf3 PE=1 SV=2)

HSP 1 Score: 578.2 bits (1489), Expect = 1.1e-163
Identity = 299/595 (50.25%), Postives = 415/595 (69.75%), Query Frame = 0

Query: 1   MTYATKAIYKLLLSDFVKVSKVSVEDMLYDEQDINRSMDKIEVIDFHQTVEVNGIRFWCY 60
           MT+ATKAIY+ LLSD+VKVS +S +DMLY E D+  SMDKIE I+FH+  EV GI+FWCY
Sbjct: 95  MTHATKAIYRWLLSDYVKVSNISADDMLYTETDLEESMDKIETINFHEVKEVAGIKFWCY 154

Query: 61  TAGHVLGAAMFMVDIAGVRVLYTGDYSREEDRHLRAAEMPQFSPDVCIIESTYGVQLHQP 120
            AGHVLGAAMFM++IAGV++LYTGD+SR+EDRHL AAE+P   PD+ IIESTYG  +H+ 
Sbjct: 155 HAGHVLGAAMFMIEIAGVKLLYTGDFSRQEDRHLMAAEIPNIKPDILIIESTYGTHIHEK 214

Query: 121 RHIREKRFTDVVHSTISQGGRVLIPAFALGRAQELLLILDEYWANHPELHNIPIYYASPL 180
           R  RE RF + VH  +++GGR LIP FALGRAQELLLILDEYW NHPELH+IPIYYAS L
Sbjct: 215 REEREARFCNTVHDIVNRGGRGLIPVFALGRAQELLLILDEYWQNHPELHDIPIYYASSL 274

Query: 181 AKRCLTVYETYTLSMNDRI--QNAKSNPFRFKYISPLKSIEVFKDVGPSVVMASPGGLQS 240
           AK+C+ VY+TY  +MND+I  Q   +NPF FK+IS LKS++ F D+GPSVVMASPG +Q+
Sbjct: 275 AKKCMAVYQTYVNAMNDKIRKQININNPFVFKHISNLKSMDHFDDIGPSVVMASPGMIQN 334

Query: 241 GLSRQLFDMWCSDKKNSCVLPGYVVEGTLAKTIINEPKEVTLMSGLMAPLNMQVHYISFS 300
           GLSR+LF+ WC+DK+N  ++ GY VEGTLAK I++EP+E+T MSG   PL M V YISFS
Sbjct: 335 GLSRELFESWCTDKRNGVIIAGYCVEGTLAKHIMSEPEEITTMSGQKLPLKMSVDYISFS 394

Query: 301 AHADFAQTSAFLEELMPPNIILVHGEANEMGRLKQKLMSQFADR---NTKILTPKNCQSV 360
           AH D+ QTS F+  L PP++ILVHGE NEM RLK  L+ ++ D    + ++  P+N ++V
Sbjct: 395 AHTDYQQTSEFIRALKPPHVILVHGEQNEMARLKAALIREYEDNDEVHIEVHNPRNTEAV 454

Query: 361 EMYFNSQKMAKTIGKLAEKTPDVGETVSGLLVKKGFAYQIMAPEDLHIFSQLSTANINQR 420
            + F  +K+AK +G LA+K P+ G+ VSG+LVK+ F Y I++P DL  ++ L+ + + Q 
Sbjct: 455 TLNFRGEKLAKVMGFLADKKPEQGQRVSGILVKRNFNYHILSPCDLSNYTDLAMSTVKQT 514

Query: 421 ITIPYSNAFNVIVRRLKQVYESVESSTDDESGVPMIRVHDRVTVKQESEKHVSLHWTSDP 480
             IPY+  F ++  +L+++   VE     E   P ++V   +TV QE    V L W ++P
Sbjct: 515 QAIPYTGPFYLLYYQLQKLTGDVEELEIQEK--PALKVFKSITVVQEPGM-VVLEWLANP 574

Query: 481 LSDMVSDSVVALILNI--NREVPKVIVESEAVKTEEENVKKAEKVIHALLVSLFGD--VK 540
            +DM +D+V  +IL +  N ++ K  V+  + K E   +    K +  +L  +FG+  V 
Sbjct: 575 SNDMYADTVTTVILEVQSNPKIRKGAVQKVSKKLE---MHVYSKRLEVMLQDIFGEDCVS 634

Query: 541 LGENGKLVINVDGNIAEVDKQSGEVE-----SDNEALKERVKTAFQRIQCAVNPI 582
           + ++  L + VDG  A ++ ++  VE      D+E+L+E V+ A QR+  A+ P+
Sbjct: 635 VKDDSVLSVTVDGKTANINLETRAVECEEGSEDDESLREMVELAAQRLYEALTPV 683

BLAST of Cucsat.G10004 vs. ExPASy Swiss-Prot
Match: Q86A79 (Cleavage and polyadenylation specificity factor subunit 3 OS=Dictyostelium discoideum OX=44689 GN=cpsf3 PE=3 SV=1)

HSP 1 Score: 554.3 bits (1427), Expect = 1.7e-156
Identity = 287/633 (45.34%), Postives = 414/633 (65.40%), Query Frame = 0

Query: 1   MTYATKAIYKLLLSDFVKVSKVS-VEDMLYDEQDINRSMDKIEVIDFHQTVEVNGIRFWC 60
           MT+ TKAIY +LLSD+VKVS ++  +DML+D+ D++RS++KIE + + Q VE NGI+  C
Sbjct: 121 MTHPTKAIYGMLLSDYVKVSNITRDDDMLFDKSDLDRSLEKIEKVRYRQKVEHNGIKVTC 180

Query: 61  YTAGHVLGAAMFMVDIAGVRVLYTGDYSREEDRHLRAAEMPQFSPDVCIIESTYGVQLHQ 120
           + AGHVLGAAMFM++IAGV++LYTGD+SR+EDRHL  AE P    DV IIESTYGVQ+H+
Sbjct: 181 FNAGHVLGAAMFMIEIAGVKILYTGDFSRQEDRHLMGAETPPVKVDVLIIESTYGVQVHE 240

Query: 121 PRHIREKRFTDVVHSTISQGGRVLIPAFALGRAQELLLILDEYWANHPELHNIPIYYASP 180
           PR  REKRFT  VH  + + G+ LIP FALGRAQELLLILDEYW  +P+LH++PIYYAS 
Sbjct: 241 PRLEREKRFTSSVHQVVERNGKCLIPVFALGRAQELLLILDEYWIANPQLHHVPIYYASA 300

Query: 181 LAKRCLTVYETYTLSMNDRI--QNAKSNPFRFKYISPLKSIEVFKDVGPSVVMASPGGLQ 240
           LAK+C+ VY TY   MNDR+  Q   SNPF FK+I  +K IE F D GP V MASPG LQ
Sbjct: 301 LAKKCMGVYRTYINMMNDRVRAQFDVSNPFEFKHIKNIKGIESFDDRGPCVFMASPGMLQ 360

Query: 241 SGLSRQLFDMWCSDKKNSCVLPGYVVEGTLAKTIINEPKEVTLMSGLMAPLNMQVHYISF 300
           SGLSRQLF+ WCSDK+N  V+PGY VEGTLAK I++EP E+T +  +  PLN+ V Y+SF
Sbjct: 361 SGLSRQLFERWCSDKRNGIVIPGYSVEGTLAKHIMSEPAEITRLDNVNVPLNLTVSYVSF 420

Query: 301 SAHADFAQTSAFLEELMPPNIILVHGEANEMGRLKQKLMSQFADRNTKILTPKNCQSVEM 360
           SAH+DF QTS F++E+ PP+++LVHG+ANEM RL+Q L+++F  +   +LTPKN  SV +
Sbjct: 421 SAHSDFLQTSEFIQEIQPPHVVLVHGDANEMSRLRQSLVAKF--KTINVLTPKNAMSVAL 480

Query: 361 YFNSQKMAKTIGKLAEKTPDVGETVSGLLVKKGFAYQIMAPEDLHIFSQLSTANINQRIT 420
            F  +K+AKT+G +    P   + + G+LV K F + I++  D+H ++ L T  I Q++T
Sbjct: 481 EFRPEKVAKTLGSIITNPPKQNDIIQGILVTKDFTHHILSASDIHNYTNLKTNIIKQKLT 540

Query: 421 IPYSNAFNVIVRRLKQVYESVESSTDDESG----VPMIRVHDRVTVKQESEKHVSLHWTS 480
           +P++  +++++  L+Q+YE +  ST+   G     P I +++ + +       + L W S
Sbjct: 541 LPFAQTYHILISTLEQIYEQIIESTESTGGGGNEKPTITIYNEIKLIYNIGVSIILEWNS 600

Query: 481 DPLSDMVSDSVVALILNI-------------------NREVPKVIVESEAVKTEEEN--- 540
           + ++DM+ DS++ALI  I                     E+ K  +E E  K +E+    
Sbjct: 601 NTVNDMICDSIIALISQIELNPLSIKVRNPNFNNIDEKEEITKDDIEKEKEKEKEQQDGD 660

Query: 541 ------------VKKAEKV---------IHALLVSLFGDVKLGENGKLVI--NVDGNIAE 582
                        +K+ K+         +  LL   +G+ K+ EN  L++  N+D   A 
Sbjct: 661 DDDDDEIQIKVVSRKSRKLSNKLNTITEVKLLLEQQYGNFKVDENDPLILHFNLDNQKAI 720

BLAST of Cucsat.G10004 vs. NCBI nr
Match: XP_011655552.2 (cleavage and polyadenylation specificity factor subunit 3-I-like [Cucumis sativus])

HSP 1 Score: 1155 bits (2988), Expect = 0.0
Identity = 587/587 (100.00%), Postives = 587/587 (100.00%), Query Frame = 0

Query: 1   MTYATKAIYKLLLSDFVKVSKVSVEDMLYDEQDINRSMDKIEVIDFHQTVEVNGIRFWCY 60
           MTYATKAIYKLLLSDFVKVSKVSVEDMLYDEQDINRSMDKIEVIDFHQTVEVNGIRFWCY
Sbjct: 107 MTYATKAIYKLLLSDFVKVSKVSVEDMLYDEQDINRSMDKIEVIDFHQTVEVNGIRFWCY 166

Query: 61  TAGHVLGAAMFMVDIAGVRVLYTGDYSREEDRHLRAAEMPQFSPDVCIIESTYGVQLHQP 120
           TAGHVLGAAMFMVDIAGVRVLYTGDYSREEDRHLRAAEMPQFSPDVCIIESTYGVQLHQP
Sbjct: 167 TAGHVLGAAMFMVDIAGVRVLYTGDYSREEDRHLRAAEMPQFSPDVCIIESTYGVQLHQP 226

Query: 121 RHIREKRFTDVVHSTISQGGRVLIPAFALGRAQELLLILDEYWANHPELHNIPIYYASPL 180
           RHIREKRFTDVVHSTISQGGRVLIPAFALGRAQELLLILDEYWANHPELHNIPIYYASPL
Sbjct: 227 RHIREKRFTDVVHSTISQGGRVLIPAFALGRAQELLLILDEYWANHPELHNIPIYYASPL 286

Query: 181 AKRCLTVYETYTLSMNDRIQNAKSNPFRFKYISPLKSIEVFKDVGPSVVMASPGGLQSGL 240
           AKRCLTVYETYTLSMNDRIQNAKSNPFRFKYISPLKSIEVFKDVGPSVVMASPGGLQSGL
Sbjct: 287 AKRCLTVYETYTLSMNDRIQNAKSNPFRFKYISPLKSIEVFKDVGPSVVMASPGGLQSGL 346

Query: 241 SRQLFDMWCSDKKNSCVLPGYVVEGTLAKTIINEPKEVTLMSGLMAPLNMQVHYISFSAH 300
           SRQLFDMWCSDKKNSCVLPGYVVEGTLAKTIINEPKEVTLMSGLMAPLNMQVHYISFSAH
Sbjct: 347 SRQLFDMWCSDKKNSCVLPGYVVEGTLAKTIINEPKEVTLMSGLMAPLNMQVHYISFSAH 406

Query: 301 ADFAQTSAFLEELMPPNIILVHGEANEMGRLKQKLMSQFADRNTKILTPKNCQSVEMYFN 360
           ADFAQTSAFLEELMPPNIILVHGEANEMGRLKQKLMSQFADRNTKILTPKNCQSVEMYFN
Sbjct: 407 ADFAQTSAFLEELMPPNIILVHGEANEMGRLKQKLMSQFADRNTKILTPKNCQSVEMYFN 466

Query: 361 SQKMAKTIGKLAEKTPDVGETVSGLLVKKGFAYQIMAPEDLHIFSQLSTANINQRITIPY 420
           SQKMAKTIGKLAEKTPDVGETVSGLLVKKGFAYQIMAPEDLHIFSQLSTANINQRITIPY
Sbjct: 467 SQKMAKTIGKLAEKTPDVGETVSGLLVKKGFAYQIMAPEDLHIFSQLSTANINQRITIPY 526

Query: 421 SNAFNVIVRRLKQVYESVESSTDDESGVPMIRVHDRVTVKQESEKHVSLHWTSDPLSDMV 480
           SNAFNVIVRRLKQVYESVESSTDDESGVPMIRVHDRVTVKQESEKHVSLHWTSDPLSDMV
Sbjct: 527 SNAFNVIVRRLKQVYESVESSTDDESGVPMIRVHDRVTVKQESEKHVSLHWTSDPLSDMV 586

Query: 481 SDSVVALILNINREVPKVIVESEAVKTEEENVKKAEKVIHALLVSLFGDVKLGENGKLVI 540
           SDSVVALILNINREVPKVIVESEAVKTEEENVKKAEKVIHALLVSLFGDVKLGENGKLVI
Sbjct: 587 SDSVVALILNINREVPKVIVESEAVKTEEENVKKAEKVIHALLVSLFGDVKLGENGKLVI 646

Query: 541 NVDGNIAEVDKQSGEVESDNEALKERVKTAFQRIQCAVNPIPLSSSS 587
           NVDGNIAEVDKQSGEVESDNEALKERVKTAFQRIQCAVNPIPLSSSS
Sbjct: 647 NVDGNIAEVDKQSGEVESDNEALKERVKTAFQRIQCAVNPIPLSSSS 693

BLAST of Cucsat.G10004 vs. NCBI nr
Match: KAE8648661.1 (hypothetical protein Csa_008894, partial [Cucumis sativus])

HSP 1 Score: 1155 bits (2988), Expect = 0.0
Identity = 587/587 (100.00%), Postives = 587/587 (100.00%), Query Frame = 0

Query: 1   MTYATKAIYKLLLSDFVKVSKVSVEDMLYDEQDINRSMDKIEVIDFHQTVEVNGIRFWCY 60
           MTYATKAIYKLLLSDFVKVSKVSVEDMLYDEQDINRSMDKIEVIDFHQTVEVNGIRFWCY
Sbjct: 9   MTYATKAIYKLLLSDFVKVSKVSVEDMLYDEQDINRSMDKIEVIDFHQTVEVNGIRFWCY 68

Query: 61  TAGHVLGAAMFMVDIAGVRVLYTGDYSREEDRHLRAAEMPQFSPDVCIIESTYGVQLHQP 120
           TAGHVLGAAMFMVDIAGVRVLYTGDYSREEDRHLRAAEMPQFSPDVCIIESTYGVQLHQP
Sbjct: 69  TAGHVLGAAMFMVDIAGVRVLYTGDYSREEDRHLRAAEMPQFSPDVCIIESTYGVQLHQP 128

Query: 121 RHIREKRFTDVVHSTISQGGRVLIPAFALGRAQELLLILDEYWANHPELHNIPIYYASPL 180
           RHIREKRFTDVVHSTISQGGRVLIPAFALGRAQELLLILDEYWANHPELHNIPIYYASPL
Sbjct: 129 RHIREKRFTDVVHSTISQGGRVLIPAFALGRAQELLLILDEYWANHPELHNIPIYYASPL 188

Query: 181 AKRCLTVYETYTLSMNDRIQNAKSNPFRFKYISPLKSIEVFKDVGPSVVMASPGGLQSGL 240
           AKRCLTVYETYTLSMNDRIQNAKSNPFRFKYISPLKSIEVFKDVGPSVVMASPGGLQSGL
Sbjct: 189 AKRCLTVYETYTLSMNDRIQNAKSNPFRFKYISPLKSIEVFKDVGPSVVMASPGGLQSGL 248

Query: 241 SRQLFDMWCSDKKNSCVLPGYVVEGTLAKTIINEPKEVTLMSGLMAPLNMQVHYISFSAH 300
           SRQLFDMWCSDKKNSCVLPGYVVEGTLAKTIINEPKEVTLMSGLMAPLNMQVHYISFSAH
Sbjct: 249 SRQLFDMWCSDKKNSCVLPGYVVEGTLAKTIINEPKEVTLMSGLMAPLNMQVHYISFSAH 308

Query: 301 ADFAQTSAFLEELMPPNIILVHGEANEMGRLKQKLMSQFADRNTKILTPKNCQSVEMYFN 360
           ADFAQTSAFLEELMPPNIILVHGEANEMGRLKQKLMSQFADRNTKILTPKNCQSVEMYFN
Sbjct: 309 ADFAQTSAFLEELMPPNIILVHGEANEMGRLKQKLMSQFADRNTKILTPKNCQSVEMYFN 368

Query: 361 SQKMAKTIGKLAEKTPDVGETVSGLLVKKGFAYQIMAPEDLHIFSQLSTANINQRITIPY 420
           SQKMAKTIGKLAEKTPDVGETVSGLLVKKGFAYQIMAPEDLHIFSQLSTANINQRITIPY
Sbjct: 369 SQKMAKTIGKLAEKTPDVGETVSGLLVKKGFAYQIMAPEDLHIFSQLSTANINQRITIPY 428

Query: 421 SNAFNVIVRRLKQVYESVESSTDDESGVPMIRVHDRVTVKQESEKHVSLHWTSDPLSDMV 480
           SNAFNVIVRRLKQVYESVESSTDDESGVPMIRVHDRVTVKQESEKHVSLHWTSDPLSDMV
Sbjct: 429 SNAFNVIVRRLKQVYESVESSTDDESGVPMIRVHDRVTVKQESEKHVSLHWTSDPLSDMV 488

Query: 481 SDSVVALILNINREVPKVIVESEAVKTEEENVKKAEKVIHALLVSLFGDVKLGENGKLVI 540
           SDSVVALILNINREVPKVIVESEAVKTEEENVKKAEKVIHALLVSLFGDVKLGENGKLVI
Sbjct: 489 SDSVVALILNINREVPKVIVESEAVKTEEENVKKAEKVIHALLVSLFGDVKLGENGKLVI 548

Query: 541 NVDGNIAEVDKQSGEVESDNEALKERVKTAFQRIQCAVNPIPLSSSS 587
           NVDGNIAEVDKQSGEVESDNEALKERVKTAFQRIQCAVNPIPLSSSS
Sbjct: 549 NVDGNIAEVDKQSGEVESDNEALKERVKTAFQRIQCAVNPIPLSSSS 595

BLAST of Cucsat.G10004 vs. NCBI nr
Match: XP_008465568.2 (PREDICTED: cleavage and polyadenylation specificity factor subunit 3-I isoform X1 [Cucumis melo] >XP_016903423.1 PREDICTED: cleavage and polyadenylation specificity factor subunit 3-I isoform X1 [Cucumis melo])

HSP 1 Score: 1150 bits (2975), Expect = 0.0
Identity = 583/587 (99.32%), Postives = 587/587 (100.00%), Query Frame = 0

Query: 1   MTYATKAIYKLLLSDFVKVSKVSVEDMLYDEQDINRSMDKIEVIDFHQTVEVNGIRFWCY 60
           MTYATKAIYKLLLSDFVKVSKVSVEDMLYDEQDINRSMDKIEVIDFHQTVEVNGIRFWCY
Sbjct: 1   MTYATKAIYKLLLSDFVKVSKVSVEDMLYDEQDINRSMDKIEVIDFHQTVEVNGIRFWCY 60

Query: 61  TAGHVLGAAMFMVDIAGVRVLYTGDYSREEDRHLRAAEMPQFSPDVCIIESTYGVQLHQP 120
           TAGHVLGAAMFMVDIAGVRVLYTGDYSREEDRHLRAAEMPQFSPDVCIIESTYGVQLHQP
Sbjct: 61  TAGHVLGAAMFMVDIAGVRVLYTGDYSREEDRHLRAAEMPQFSPDVCIIESTYGVQLHQP 120

Query: 121 RHIREKRFTDVVHSTISQGGRVLIPAFALGRAQELLLILDEYWANHPELHNIPIYYASPL 180
           RHIREKRFTDVVHSTISQGGRVLIPAFALGRAQELLLILDEYWANHPELHNIPIYYASPL
Sbjct: 121 RHIREKRFTDVVHSTISQGGRVLIPAFALGRAQELLLILDEYWANHPELHNIPIYYASPL 180

Query: 181 AKRCLTVYETYTLSMNDRIQNAKSNPFRFKYISPLKSIEVFKDVGPSVVMASPGGLQSGL 240
           AKRCLTVYETYTLSMNDRIQNAKSNPFRFKYISPLKSIEVFKDVGPSVVMASPGGLQSGL
Sbjct: 181 AKRCLTVYETYTLSMNDRIQNAKSNPFRFKYISPLKSIEVFKDVGPSVVMASPGGLQSGL 240

Query: 241 SRQLFDMWCSDKKNSCVLPGYVVEGTLAKTIINEPKEVTLMSGLMAPLNMQVHYISFSAH 300
           SRQLFDMWCSDKKNSCV+PGYVVEGTLAKTIINEPKEVTLMSGLMAPLNMQVHYISFSAH
Sbjct: 241 SRQLFDMWCSDKKNSCVIPGYVVEGTLAKTIINEPKEVTLMSGLMAPLNMQVHYISFSAH 300

Query: 301 ADFAQTSAFLEELMPPNIILVHGEANEMGRLKQKLMSQFADRNTKILTPKNCQSVEMYFN 360
           ADFAQTSAFLEELMPPNIILVHGEANEMGRLKQKLMSQFADRNTKILTPKNCQSVEMYFN
Sbjct: 301 ADFAQTSAFLEELMPPNIILVHGEANEMGRLKQKLMSQFADRNTKILTPKNCQSVEMYFN 360

Query: 361 SQKMAKTIGKLAEKTPDVGETVSGLLVKKGFAYQIMAPEDLHIFSQLSTANINQRITIPY 420
           SQKMAKTIGKLAEKTP+VGETVSGLLVKKGFAYQIMAPEDLHIFSQLSTANINQRITIPY
Sbjct: 361 SQKMAKTIGKLAEKTPEVGETVSGLLVKKGFAYQIMAPEDLHIFSQLSTANINQRITIPY 420

Query: 421 SNAFNVIVRRLKQVYESVESSTDDESGVPMIRVHDRVTVKQESEKHVSLHWTSDPLSDMV 480
           SNAFNVIVRRLKQVYESVESSTDDESGVPMIRVHDRVTVKQESEKHVSLHWTSDPLSDMV
Sbjct: 421 SNAFNVIVRRLKQVYESVESSTDDESGVPMIRVHDRVTVKQESEKHVSLHWTSDPLSDMV 480

Query: 481 SDSVVALILNINREVPKVIVESEAVKTEEENVKKAEKVIHALLVSLFGDVKLGENGKLVI 540
           SDSVVALILNINREVPKVIVESEAVKTEEEN+KKAEKVIHALLVSLFGDVKLGENGKLVI
Sbjct: 481 SDSVVALILNINREVPKVIVESEAVKTEEENMKKAEKVIHALLVSLFGDVKLGENGKLVI 540

Query: 541 NVDGNIAEVDKQSGEVESDNEALKERVKTAFQRIQCAVNPIPLSSSS 587
           NVDGNIAEVDKQSGEVES+NEALKERVKTAFQRIQCAVNPIPLSSSS
Sbjct: 541 NVDGNIAEVDKQSGEVESENEALKERVKTAFQRIQCAVNPIPLSSSS 587

BLAST of Cucsat.G10004 vs. NCBI nr
Match: KAA0034167.1 (cleavage and polyadenylation specificity factor subunit 3-I-like [Cucumis melo var. makuwa] >KAA0034170.1 cleavage and polyadenylation specificity factor subunit 3-I-like [Cucumis melo var. makuwa] >TYK15749.1 cleavage and polyadenylation specificity factor subunit 3-I-like [Cucumis melo var. makuwa] >TYK15752.1 cleavage and polyadenylation specificity factor subunit 3-I-like [Cucumis melo var. makuwa])

HSP 1 Score: 1150 bits (2975), Expect = 0.0
Identity = 583/587 (99.32%), Postives = 587/587 (100.00%), Query Frame = 0

Query: 1   MTYATKAIYKLLLSDFVKVSKVSVEDMLYDEQDINRSMDKIEVIDFHQTVEVNGIRFWCY 60
           MTYATKAIYKLLLSDFVKVSKVSVEDMLYDEQDINRSMDKIEVIDFHQTVEVNGIRFWCY
Sbjct: 91  MTYATKAIYKLLLSDFVKVSKVSVEDMLYDEQDINRSMDKIEVIDFHQTVEVNGIRFWCY 150

Query: 61  TAGHVLGAAMFMVDIAGVRVLYTGDYSREEDRHLRAAEMPQFSPDVCIIESTYGVQLHQP 120
           TAGHVLGAAMFMVDIAGVRVLYTGDYSREEDRHLRAAEMPQFSPDVCIIESTYGVQLHQP
Sbjct: 151 TAGHVLGAAMFMVDIAGVRVLYTGDYSREEDRHLRAAEMPQFSPDVCIIESTYGVQLHQP 210

Query: 121 RHIREKRFTDVVHSTISQGGRVLIPAFALGRAQELLLILDEYWANHPELHNIPIYYASPL 180
           RHIREKRFTDVVHSTISQGGRVLIPAFALGRAQELLLILDEYWANHPELHNIPIYYASPL
Sbjct: 211 RHIREKRFTDVVHSTISQGGRVLIPAFALGRAQELLLILDEYWANHPELHNIPIYYASPL 270

Query: 181 AKRCLTVYETYTLSMNDRIQNAKSNPFRFKYISPLKSIEVFKDVGPSVVMASPGGLQSGL 240
           AKRCLTVYETYTLSMNDRIQNAKSNPFRFKYISPLKSIEVFKDVGPSVVMASPGGLQSGL
Sbjct: 271 AKRCLTVYETYTLSMNDRIQNAKSNPFRFKYISPLKSIEVFKDVGPSVVMASPGGLQSGL 330

Query: 241 SRQLFDMWCSDKKNSCVLPGYVVEGTLAKTIINEPKEVTLMSGLMAPLNMQVHYISFSAH 300
           SRQLFDMWCSDKKNSCV+PGYVVEGTLAKTIINEPKEVTLMSGLMAPLNMQVHYISFSAH
Sbjct: 331 SRQLFDMWCSDKKNSCVIPGYVVEGTLAKTIINEPKEVTLMSGLMAPLNMQVHYISFSAH 390

Query: 301 ADFAQTSAFLEELMPPNIILVHGEANEMGRLKQKLMSQFADRNTKILTPKNCQSVEMYFN 360
           ADFAQTSAFLEELMPPNIILVHGEANEMGRLKQKLMSQFADRNTKILTPKNCQSVEMYFN
Sbjct: 391 ADFAQTSAFLEELMPPNIILVHGEANEMGRLKQKLMSQFADRNTKILTPKNCQSVEMYFN 450

Query: 361 SQKMAKTIGKLAEKTPDVGETVSGLLVKKGFAYQIMAPEDLHIFSQLSTANINQRITIPY 420
           SQKMAKTIGKLAEKTP+VGETVSGLLVKKGFAYQIMAPEDLHIFSQLSTANINQRITIPY
Sbjct: 451 SQKMAKTIGKLAEKTPEVGETVSGLLVKKGFAYQIMAPEDLHIFSQLSTANINQRITIPY 510

Query: 421 SNAFNVIVRRLKQVYESVESSTDDESGVPMIRVHDRVTVKQESEKHVSLHWTSDPLSDMV 480
           SNAFNVIVRRLKQVYESVESSTDDESGVPMIRVHDRVTVKQESEKHVSLHWTSDPLSDMV
Sbjct: 511 SNAFNVIVRRLKQVYESVESSTDDESGVPMIRVHDRVTVKQESEKHVSLHWTSDPLSDMV 570

Query: 481 SDSVVALILNINREVPKVIVESEAVKTEEENVKKAEKVIHALLVSLFGDVKLGENGKLVI 540
           SDSVVALILNINREVPKVIVESEAVKTEEEN+KKAEKVIHALLVSLFGDVKLGENGKLVI
Sbjct: 571 SDSVVALILNINREVPKVIVESEAVKTEEENMKKAEKVIHALLVSLFGDVKLGENGKLVI 630

Query: 541 NVDGNIAEVDKQSGEVESDNEALKERVKTAFQRIQCAVNPIPLSSSS 587
           NVDGNIAEVDKQSGEVES+NEALKERVKTAFQRIQCAVNPIPLSSSS
Sbjct: 631 NVDGNIAEVDKQSGEVESENEALKERVKTAFQRIQCAVNPIPLSSSS 677

BLAST of Cucsat.G10004 vs. NCBI nr
Match: XP_011655548.2 (cleavage and polyadenylation specificity factor subunit 3-I isoform X1 [Cucumis sativus] >XP_031741341.1 cleavage and polyadenylation specificity factor subunit 3-I isoform X1 [Cucumis sativus])

HSP 1 Score: 1145 bits (2963), Expect = 0.0
Identity = 582/587 (99.15%), Postives = 585/587 (99.66%), Query Frame = 0

Query: 1   MTYATKAIYKLLLSDFVKVSKVSVEDMLYDEQDINRSMDKIEVIDFHQTVEVNGIRFWCY 60
           MTYATKAIYKLLL DFVKVSKVSVEDMLYDEQDI+RSMDKIEVIDFHQTVEVNGIRFWCY
Sbjct: 107 MTYATKAIYKLLLLDFVKVSKVSVEDMLYDEQDISRSMDKIEVIDFHQTVEVNGIRFWCY 166

Query: 61  TAGHVLGAAMFMVDIAGVRVLYTGDYSREEDRHLRAAEMPQFSPDVCIIESTYGVQLHQP 120
           TAGHVLGAAMFMVDIAGVRVLYTGDYSREEDRHLRAAEMPQFSPDVCIIESTYGVQLHQP
Sbjct: 167 TAGHVLGAAMFMVDIAGVRVLYTGDYSREEDRHLRAAEMPQFSPDVCIIESTYGVQLHQP 226

Query: 121 RHIREKRFTDVVHSTISQGGRVLIPAFALGRAQELLLILDEYWANHPELHNIPIYYASPL 180
           RHIREKRFTDVVHSTISQGGRVLIPAFALGRAQELLLILDEYWANHPELHNIPIYYASPL
Sbjct: 227 RHIREKRFTDVVHSTISQGGRVLIPAFALGRAQELLLILDEYWANHPELHNIPIYYASPL 286

Query: 181 AKRCLTVYETYTLSMNDRIQNAKSNPFRFKYISPLKSIEVFKDVGPSVVMASPGGLQSGL 240
           AKRCLTVYETYTLSMNDRIQNAKSNPFRFKYISPLKSIEVFKDVGPSVVMASP GLQSGL
Sbjct: 287 AKRCLTVYETYTLSMNDRIQNAKSNPFRFKYISPLKSIEVFKDVGPSVVMASPSGLQSGL 346

Query: 241 SRQLFDMWCSDKKNSCVLPGYVVEGTLAKTIINEPKEVTLMSGLMAPLNMQVHYISFSAH 300
           SRQLF+MWCSDKKNSCVLPGYVVEGTLAKTIINEPKEVTLMSGLMAPLNMQVHYISFSAH
Sbjct: 347 SRQLFEMWCSDKKNSCVLPGYVVEGTLAKTIINEPKEVTLMSGLMAPLNMQVHYISFSAH 406

Query: 301 ADFAQTSAFLEELMPPNIILVHGEANEMGRLKQKLMSQFADRNTKILTPKNCQSVEMYFN 360
           ADFAQTSAFLEELMPPNIILVHGEANEMGRLKQKLMSQFADRNTKILTPKNCQSVEMYFN
Sbjct: 407 ADFAQTSAFLEELMPPNIILVHGEANEMGRLKQKLMSQFADRNTKILTPKNCQSVEMYFN 466

Query: 361 SQKMAKTIGKLAEKTPDVGETVSGLLVKKGFAYQIMAPEDLHIFSQLSTANINQRITIPY 420
           SQKMAKTIGKLAEKTPDVGETVSGLLVKKGFAYQIMAPEDLHIFSQLSTANINQRITIPY
Sbjct: 467 SQKMAKTIGKLAEKTPDVGETVSGLLVKKGFAYQIMAPEDLHIFSQLSTANINQRITIPY 526

Query: 421 SNAFNVIVRRLKQVYESVESSTDDESGVPMIRVHDRVTVKQESEKHVSLHWTSDPLSDMV 480
           SNAFNVIVRRLKQVYESVESSTDDESGVPMIRVHDRVTVKQESEKHVSLHWTSDPLSDMV
Sbjct: 527 SNAFNVIVRRLKQVYESVESSTDDESGVPMIRVHDRVTVKQESEKHVSLHWTSDPLSDMV 586

Query: 481 SDSVVALILNINREVPKVIVESEAVKTEEENVKKAEKVIHALLVSLFGDVKLGENGKLVI 540
           SDSVVALILNINREVPKVIVESEAVKTEEENVKKAEKVIHALLVSLFGDVKLGENGKLVI
Sbjct: 587 SDSVVALILNINREVPKVIVESEAVKTEEENVKKAEKVIHALLVSLFGDVKLGENGKLVI 646

Query: 541 NVDGNIAEVDKQSGEVESDNEALKERVKTAFQRIQCAVNPIPLSSSS 587
           NVDGNIAEVDKQSGEVES+NEALKERVKTAFQRIQCAVNPIPLSSSS
Sbjct: 647 NVDGNIAEVDKQSGEVESENEALKERVKTAFQRIQCAVNPIPLSSSS 693

BLAST of Cucsat.G10004 vs. ExPASy TrEMBL
Match: A0A1S4E5B2 (cleavage and polyadenylation specificity factor subunit 3-I isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503202 PE=4 SV=1)

HSP 1 Score: 1150 bits (2975), Expect = 0.0
Identity = 583/587 (99.32%), Postives = 587/587 (100.00%), Query Frame = 0

Query: 1   MTYATKAIYKLLLSDFVKVSKVSVEDMLYDEQDINRSMDKIEVIDFHQTVEVNGIRFWCY 60
           MTYATKAIYKLLLSDFVKVSKVSVEDMLYDEQDINRSMDKIEVIDFHQTVEVNGIRFWCY
Sbjct: 1   MTYATKAIYKLLLSDFVKVSKVSVEDMLYDEQDINRSMDKIEVIDFHQTVEVNGIRFWCY 60

Query: 61  TAGHVLGAAMFMVDIAGVRVLYTGDYSREEDRHLRAAEMPQFSPDVCIIESTYGVQLHQP 120
           TAGHVLGAAMFMVDIAGVRVLYTGDYSREEDRHLRAAEMPQFSPDVCIIESTYGVQLHQP
Sbjct: 61  TAGHVLGAAMFMVDIAGVRVLYTGDYSREEDRHLRAAEMPQFSPDVCIIESTYGVQLHQP 120

Query: 121 RHIREKRFTDVVHSTISQGGRVLIPAFALGRAQELLLILDEYWANHPELHNIPIYYASPL 180
           RHIREKRFTDVVHSTISQGGRVLIPAFALGRAQELLLILDEYWANHPELHNIPIYYASPL
Sbjct: 121 RHIREKRFTDVVHSTISQGGRVLIPAFALGRAQELLLILDEYWANHPELHNIPIYYASPL 180

Query: 181 AKRCLTVYETYTLSMNDRIQNAKSNPFRFKYISPLKSIEVFKDVGPSVVMASPGGLQSGL 240
           AKRCLTVYETYTLSMNDRIQNAKSNPFRFKYISPLKSIEVFKDVGPSVVMASPGGLQSGL
Sbjct: 181 AKRCLTVYETYTLSMNDRIQNAKSNPFRFKYISPLKSIEVFKDVGPSVVMASPGGLQSGL 240

Query: 241 SRQLFDMWCSDKKNSCVLPGYVVEGTLAKTIINEPKEVTLMSGLMAPLNMQVHYISFSAH 300
           SRQLFDMWCSDKKNSCV+PGYVVEGTLAKTIINEPKEVTLMSGLMAPLNMQVHYISFSAH
Sbjct: 241 SRQLFDMWCSDKKNSCVIPGYVVEGTLAKTIINEPKEVTLMSGLMAPLNMQVHYISFSAH 300

Query: 301 ADFAQTSAFLEELMPPNIILVHGEANEMGRLKQKLMSQFADRNTKILTPKNCQSVEMYFN 360
           ADFAQTSAFLEELMPPNIILVHGEANEMGRLKQKLMSQFADRNTKILTPKNCQSVEMYFN
Sbjct: 301 ADFAQTSAFLEELMPPNIILVHGEANEMGRLKQKLMSQFADRNTKILTPKNCQSVEMYFN 360

Query: 361 SQKMAKTIGKLAEKTPDVGETVSGLLVKKGFAYQIMAPEDLHIFSQLSTANINQRITIPY 420
           SQKMAKTIGKLAEKTP+VGETVSGLLVKKGFAYQIMAPEDLHIFSQLSTANINQRITIPY
Sbjct: 361 SQKMAKTIGKLAEKTPEVGETVSGLLVKKGFAYQIMAPEDLHIFSQLSTANINQRITIPY 420

Query: 421 SNAFNVIVRRLKQVYESVESSTDDESGVPMIRVHDRVTVKQESEKHVSLHWTSDPLSDMV 480
           SNAFNVIVRRLKQVYESVESSTDDESGVPMIRVHDRVTVKQESEKHVSLHWTSDPLSDMV
Sbjct: 421 SNAFNVIVRRLKQVYESVESSTDDESGVPMIRVHDRVTVKQESEKHVSLHWTSDPLSDMV 480

Query: 481 SDSVVALILNINREVPKVIVESEAVKTEEENVKKAEKVIHALLVSLFGDVKLGENGKLVI 540
           SDSVVALILNINREVPKVIVESEAVKTEEEN+KKAEKVIHALLVSLFGDVKLGENGKLVI
Sbjct: 481 SDSVVALILNINREVPKVIVESEAVKTEEENMKKAEKVIHALLVSLFGDVKLGENGKLVI 540

Query: 541 NVDGNIAEVDKQSGEVESDNEALKERVKTAFQRIQCAVNPIPLSSSS 587
           NVDGNIAEVDKQSGEVES+NEALKERVKTAFQRIQCAVNPIPLSSSS
Sbjct: 541 NVDGNIAEVDKQSGEVESENEALKERVKTAFQRIQCAVNPIPLSSSS 587

BLAST of Cucsat.G10004 vs. ExPASy TrEMBL
Match: A0A5A7SY15 (Cleavage and polyadenylation specificity factor subunit 3-I-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G002530 PE=4 SV=1)

HSP 1 Score: 1150 bits (2975), Expect = 0.0
Identity = 583/587 (99.32%), Postives = 587/587 (100.00%), Query Frame = 0

Query: 1   MTYATKAIYKLLLSDFVKVSKVSVEDMLYDEQDINRSMDKIEVIDFHQTVEVNGIRFWCY 60
           MTYATKAIYKLLLSDFVKVSKVSVEDMLYDEQDINRSMDKIEVIDFHQTVEVNGIRFWCY
Sbjct: 91  MTYATKAIYKLLLSDFVKVSKVSVEDMLYDEQDINRSMDKIEVIDFHQTVEVNGIRFWCY 150

Query: 61  TAGHVLGAAMFMVDIAGVRVLYTGDYSREEDRHLRAAEMPQFSPDVCIIESTYGVQLHQP 120
           TAGHVLGAAMFMVDIAGVRVLYTGDYSREEDRHLRAAEMPQFSPDVCIIESTYGVQLHQP
Sbjct: 151 TAGHVLGAAMFMVDIAGVRVLYTGDYSREEDRHLRAAEMPQFSPDVCIIESTYGVQLHQP 210

Query: 121 RHIREKRFTDVVHSTISQGGRVLIPAFALGRAQELLLILDEYWANHPELHNIPIYYASPL 180
           RHIREKRFTDVVHSTISQGGRVLIPAFALGRAQELLLILDEYWANHPELHNIPIYYASPL
Sbjct: 211 RHIREKRFTDVVHSTISQGGRVLIPAFALGRAQELLLILDEYWANHPELHNIPIYYASPL 270

Query: 181 AKRCLTVYETYTLSMNDRIQNAKSNPFRFKYISPLKSIEVFKDVGPSVVMASPGGLQSGL 240
           AKRCLTVYETYTLSMNDRIQNAKSNPFRFKYISPLKSIEVFKDVGPSVVMASPGGLQSGL
Sbjct: 271 AKRCLTVYETYTLSMNDRIQNAKSNPFRFKYISPLKSIEVFKDVGPSVVMASPGGLQSGL 330

Query: 241 SRQLFDMWCSDKKNSCVLPGYVVEGTLAKTIINEPKEVTLMSGLMAPLNMQVHYISFSAH 300
           SRQLFDMWCSDKKNSCV+PGYVVEGTLAKTIINEPKEVTLMSGLMAPLNMQVHYISFSAH
Sbjct: 331 SRQLFDMWCSDKKNSCVIPGYVVEGTLAKTIINEPKEVTLMSGLMAPLNMQVHYISFSAH 390

Query: 301 ADFAQTSAFLEELMPPNIILVHGEANEMGRLKQKLMSQFADRNTKILTPKNCQSVEMYFN 360
           ADFAQTSAFLEELMPPNIILVHGEANEMGRLKQKLMSQFADRNTKILTPKNCQSVEMYFN
Sbjct: 391 ADFAQTSAFLEELMPPNIILVHGEANEMGRLKQKLMSQFADRNTKILTPKNCQSVEMYFN 450

Query: 361 SQKMAKTIGKLAEKTPDVGETVSGLLVKKGFAYQIMAPEDLHIFSQLSTANINQRITIPY 420
           SQKMAKTIGKLAEKTP+VGETVSGLLVKKGFAYQIMAPEDLHIFSQLSTANINQRITIPY
Sbjct: 451 SQKMAKTIGKLAEKTPEVGETVSGLLVKKGFAYQIMAPEDLHIFSQLSTANINQRITIPY 510

Query: 421 SNAFNVIVRRLKQVYESVESSTDDESGVPMIRVHDRVTVKQESEKHVSLHWTSDPLSDMV 480
           SNAFNVIVRRLKQVYESVESSTDDESGVPMIRVHDRVTVKQESEKHVSLHWTSDPLSDMV
Sbjct: 511 SNAFNVIVRRLKQVYESVESSTDDESGVPMIRVHDRVTVKQESEKHVSLHWTSDPLSDMV 570

Query: 481 SDSVVALILNINREVPKVIVESEAVKTEEENVKKAEKVIHALLVSLFGDVKLGENGKLVI 540
           SDSVVALILNINREVPKVIVESEAVKTEEEN+KKAEKVIHALLVSLFGDVKLGENGKLVI
Sbjct: 571 SDSVVALILNINREVPKVIVESEAVKTEEENMKKAEKVIHALLVSLFGDVKLGENGKLVI 630

Query: 541 NVDGNIAEVDKQSGEVESDNEALKERVKTAFQRIQCAVNPIPLSSSS 587
           NVDGNIAEVDKQSGEVES+NEALKERVKTAFQRIQCAVNPIPLSSSS
Sbjct: 631 NVDGNIAEVDKQSGEVESENEALKERVKTAFQRIQCAVNPIPLSSSS 677

BLAST of Cucsat.G10004 vs. ExPASy TrEMBL
Match: A0A0A0KV82 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G587170 PE=4 SV=1)

HSP 1 Score: 1145 bits (2963), Expect = 0.0
Identity = 582/587 (99.15%), Postives = 585/587 (99.66%), Query Frame = 0

Query: 1   MTYATKAIYKLLLSDFVKVSKVSVEDMLYDEQDINRSMDKIEVIDFHQTVEVNGIRFWCY 60
           MTYATKAIYKLLL DFVKVSKVSVEDMLYDEQDI+RSMDKIEVIDFHQTVEVNGIRFWCY
Sbjct: 96  MTYATKAIYKLLLLDFVKVSKVSVEDMLYDEQDISRSMDKIEVIDFHQTVEVNGIRFWCY 155

Query: 61  TAGHVLGAAMFMVDIAGVRVLYTGDYSREEDRHLRAAEMPQFSPDVCIIESTYGVQLHQP 120
           TAGHVLGAAMFMVDIAGVRVLYTGDYSREEDRHLRAAEMPQFSPDVCIIESTYGVQLHQP
Sbjct: 156 TAGHVLGAAMFMVDIAGVRVLYTGDYSREEDRHLRAAEMPQFSPDVCIIESTYGVQLHQP 215

Query: 121 RHIREKRFTDVVHSTISQGGRVLIPAFALGRAQELLLILDEYWANHPELHNIPIYYASPL 180
           RHIREKRFTDVVHSTISQGGRVLIPAFALGRAQELLLILDEYWANHPELHNIPIYYASPL
Sbjct: 216 RHIREKRFTDVVHSTISQGGRVLIPAFALGRAQELLLILDEYWANHPELHNIPIYYASPL 275

Query: 181 AKRCLTVYETYTLSMNDRIQNAKSNPFRFKYISPLKSIEVFKDVGPSVVMASPGGLQSGL 240
           AKRCLTVYETYTLSMNDRIQNAKSNPFRFKYISPLKSIEVFKDVGPSVVMASP GLQSGL
Sbjct: 276 AKRCLTVYETYTLSMNDRIQNAKSNPFRFKYISPLKSIEVFKDVGPSVVMASPSGLQSGL 335

Query: 241 SRQLFDMWCSDKKNSCVLPGYVVEGTLAKTIINEPKEVTLMSGLMAPLNMQVHYISFSAH 300
           SRQLF+MWCSDKKNSCVLPGYVVEGTLAKTIINEPKEVTLMSGLMAPLNMQVHYISFSAH
Sbjct: 336 SRQLFEMWCSDKKNSCVLPGYVVEGTLAKTIINEPKEVTLMSGLMAPLNMQVHYISFSAH 395

Query: 301 ADFAQTSAFLEELMPPNIILVHGEANEMGRLKQKLMSQFADRNTKILTPKNCQSVEMYFN 360
           ADFAQTSAFLEELMPPNIILVHGEANEMGRLKQKLMSQFADRNTKILTPKNCQSVEMYFN
Sbjct: 396 ADFAQTSAFLEELMPPNIILVHGEANEMGRLKQKLMSQFADRNTKILTPKNCQSVEMYFN 455

Query: 361 SQKMAKTIGKLAEKTPDVGETVSGLLVKKGFAYQIMAPEDLHIFSQLSTANINQRITIPY 420
           SQKMAKTIGKLAEKTPDVGETVSGLLVKKGFAYQIMAPEDLHIFSQLSTANINQRITIPY
Sbjct: 456 SQKMAKTIGKLAEKTPDVGETVSGLLVKKGFAYQIMAPEDLHIFSQLSTANINQRITIPY 515

Query: 421 SNAFNVIVRRLKQVYESVESSTDDESGVPMIRVHDRVTVKQESEKHVSLHWTSDPLSDMV 480
           SNAFNVIVRRLKQVYESVESSTDDESGVPMIRVHDRVTVKQESEKHVSLHWTSDPLSDMV
Sbjct: 516 SNAFNVIVRRLKQVYESVESSTDDESGVPMIRVHDRVTVKQESEKHVSLHWTSDPLSDMV 575

Query: 481 SDSVVALILNINREVPKVIVESEAVKTEEENVKKAEKVIHALLVSLFGDVKLGENGKLVI 540
           SDSVVALILNINREVPKVIVESEAVKTEEENVKKAEKVIHALLVSLFGDVKLGENGKLVI
Sbjct: 576 SDSVVALILNINREVPKVIVESEAVKTEEENVKKAEKVIHALLVSLFGDVKLGENGKLVI 635

Query: 541 NVDGNIAEVDKQSGEVESDNEALKERVKTAFQRIQCAVNPIPLSSSS 587
           NVDGNIAEVDKQSGEVES+NEALKERVKTAFQRIQCAVNPIPLSSSS
Sbjct: 636 NVDGNIAEVDKQSGEVESENEALKERVKTAFQRIQCAVNPIPLSSSS 682

BLAST of Cucsat.G10004 vs. ExPASy TrEMBL
Match: A0A6J1DE61 (cleavage and polyadenylation specificity factor subunit 3-I OS=Momordica charantia OX=3673 GN=LOC111019708 PE=4 SV=1)

HSP 1 Score: 1115 bits (2884), Expect = 0.0
Identity = 564/586 (96.25%), Postives = 576/586 (98.29%), Query Frame = 0

Query: 1   MTYATKAIYKLLLSDFVKVSKVSVEDMLYDEQDINRSMDKIEVIDFHQTVEVNGIRFWCY 60
           MTYATKAIYKLLLSDFVKVSKVSVEDMLYDEQDINRSMDKIEVIDFHQTVEVNGIRFWCY
Sbjct: 107 MTYATKAIYKLLLSDFVKVSKVSVEDMLYDEQDINRSMDKIEVIDFHQTVEVNGIRFWCY 166

Query: 61  TAGHVLGAAMFMVDIAGVRVLYTGDYSREEDRHLRAAEMPQFSPDVCIIESTYGVQLHQP 120
           TAGHVLGAAMFMVDIAGVRVLYTGDYSREEDRHLRAAEMPQFSPDVCIIESTYGVQLHQP
Sbjct: 167 TAGHVLGAAMFMVDIAGVRVLYTGDYSREEDRHLRAAEMPQFSPDVCIIESTYGVQLHQP 226

Query: 121 RHIREKRFTDVVHSTISQGGRVLIPAFALGRAQELLLILDEYWANHPELHNIPIYYASPL 180
           RHIREKRFTDVVHSTISQGGRVLIPAFALGRAQELLLILDEYWANHPELHNIPIYYASPL
Sbjct: 227 RHIREKRFTDVVHSTISQGGRVLIPAFALGRAQELLLILDEYWANHPELHNIPIYYASPL 286

Query: 181 AKRCLTVYETYTLSMNDRIQNAKSNPFRFKYISPLKSIEVFKDVGPSVVMASPGGLQSGL 240
           AKRCLTVYETYTLSMNDRIQNAKSNPFRFKYISPLKSIEVFKDVGPSVVMASPGGLQSGL
Sbjct: 287 AKRCLTVYETYTLSMNDRIQNAKSNPFRFKYISPLKSIEVFKDVGPSVVMASPGGLQSGL 346

Query: 241 SRQLFDMWCSDKKNSCVLPGYVVEGTLAKTIINEPKEVTLMSGLMAPLNMQVHYISFSAH 300
           SRQLFDMWCSDKKN+CVLPGYVVEGTLAKTIINEPKEVTLM+GLMAPLNMQVHYISFSAH
Sbjct: 347 SRQLFDMWCSDKKNACVLPGYVVEGTLAKTIINEPKEVTLMNGLMAPLNMQVHYISFSAH 406

Query: 301 ADFAQTSAFLEELMPPNIILVHGEANEMGRLKQKLMSQFADRNTKILTPKNCQSVEMYFN 360
           ADFAQTSAFLEELMPPNIILVHGEANEMGRLKQKL+SQFADRNTKILTPKNCQSVEMYFN
Sbjct: 407 ADFAQTSAFLEELMPPNIILVHGEANEMGRLKQKLISQFADRNTKILTPKNCQSVEMYFN 466

Query: 361 SQKMAKTIGKLAEKTPDVGETVSGLLVKKGFAYQIMAPEDLHIFSQLSTANINQRITIPY 420
           SQKMAKTIG+LAEKTP+ GETVSGLLVKKGFAYQIMA +DLHIFSQLSTANINQRITIPY
Sbjct: 467 SQKMAKTIGRLAEKTPEAGETVSGLLVKKGFAYQIMASDDLHIFSQLSTANINQRITIPY 526

Query: 421 SNAFNVIVRRLKQVYESVESSTDDESGVPMIRVHDRVTVKQESEKHVSLHWTSDPLSDMV 480
           SNAFNVIVRRLKQVYESVES TD+ESGVP I VHDRVTVK ESEKHVSLHWTSDPLSDMV
Sbjct: 527 SNAFNVIVRRLKQVYESVESLTDEESGVPAICVHDRVTVKHESEKHVSLHWTSDPLSDMV 586

Query: 481 SDSVVALILNINREVPKVIVESEAVKTEEENVKKAEKVIHALLVSLFGDVKLGENGKLVI 540
           SDSVVALILNINREVPKVIVESEA KTEEEN KKAEKVIHALLVSLFGDVKLGENGKLVI
Sbjct: 587 SDSVVALILNINREVPKVIVESEAAKTEEENEKKAEKVIHALLVSLFGDVKLGENGKLVI 646

Query: 541 NVDGNIAEVDKQSGEVESDNEALKERVKTAFQRIQCAVNPIPLSSS 586
           NVDG+IAE+DKQSGEVES+NE LKERVKTAF+RIQCAV PIPLS+S
Sbjct: 647 NVDGSIAELDKQSGEVESENEGLKERVKTAFRRIQCAVKPIPLSAS 692

BLAST of Cucsat.G10004 vs. ExPASy TrEMBL
Match: A0A6J1G1N4 (cleavage and polyadenylation specificity factor subunit 3-I-like OS=Cucurbita moschata OX=3662 GN=LOC111449842 PE=4 SV=1)

HSP 1 Score: 1113 bits (2880), Expect = 0.0
Identity = 561/585 (95.90%), Postives = 577/585 (98.63%), Query Frame = 0

Query: 1   MTYATKAIYKLLLSDFVKVSKVSVEDMLYDEQDINRSMDKIEVIDFHQTVEVNGIRFWCY 60
           MTYATKAIYKLLLSDFVKVSKVS+EDMLYDEQDINRSMDKIEVIDFHQTVEVNGIRFWCY
Sbjct: 107 MTYATKAIYKLLLSDFVKVSKVSIEDMLYDEQDINRSMDKIEVIDFHQTVEVNGIRFWCY 166

Query: 61  TAGHVLGAAMFMVDIAGVRVLYTGDYSREEDRHLRAAEMPQFSPDVCIIESTYGVQLHQP 120
           TAGHVLGAAMFMVDIAGVRVLYTGDYSREEDRHLRAAEMPQFSPDVCIIESTYGVQLHQP
Sbjct: 167 TAGHVLGAAMFMVDIAGVRVLYTGDYSREEDRHLRAAEMPQFSPDVCIIESTYGVQLHQP 226

Query: 121 RHIREKRFTDVVHSTISQGGRVLIPAFALGRAQELLLILDEYWANHPELHNIPIYYASPL 180
           RHIREKRFTDVVHSTISQGGRVLIPAFALGRAQELLLILDEYWANHPELHN+PIYYASPL
Sbjct: 227 RHIREKRFTDVVHSTISQGGRVLIPAFALGRAQELLLILDEYWANHPELHNVPIYYASPL 286

Query: 181 AKRCLTVYETYTLSMNDRIQNAKSNPFRFKYISPLKSIEVFKDVGPSVVMASPGGLQSGL 240
           AKRCLTVYETYTLSMNDRIQNAKSNPFRFKYISPLKSIEVFKDVGPSVVMASPGGLQSGL
Sbjct: 287 AKRCLTVYETYTLSMNDRIQNAKSNPFRFKYISPLKSIEVFKDVGPSVVMASPGGLQSGL 346

Query: 241 SRQLFDMWCSDKKNSCVLPGYVVEGTLAKTIINEPKEVTLMSGLMAPLNMQVHYISFSAH 300
           SRQLFD+WCSDKKNSCVLPGYVVEGTLAKTIINEPKEVTLMSGLMAPLNMQVHYISFSAH
Sbjct: 347 SRQLFDLWCSDKKNSCVLPGYVVEGTLAKTIINEPKEVTLMSGLMAPLNMQVHYISFSAH 406

Query: 301 ADFAQTSAFLEELMPPNIILVHGEANEMGRLKQKLMSQFADRNTKILTPKNCQSVEMYFN 360
           AD+AQTSAFLEELMPPNIILVHGEANEMGRLKQKLMS+FADRNTKILTPKNCQSVEMYFN
Sbjct: 407 ADYAQTSAFLEELMPPNIILVHGEANEMGRLKQKLMSKFADRNTKILTPKNCQSVEMYFN 466

Query: 361 SQKMAKTIGKLAEKTPDVGETVSGLLVKKGFAYQIMAPEDLHIFSQLSTANINQRITIPY 420
           SQKMAKTIGKLAE TP+V ETVSGLLVKKGFAYQIMAP+DLHIFSQLSTANI QRITIPY
Sbjct: 467 SQKMAKTIGKLAENTPEVDETVSGLLVKKGFAYQIMAPDDLHIFSQLSTANIYQRITIPY 526

Query: 421 SNAFNVIVRRLKQVYESVESSTDDESGVPMIRVHDRVTVKQESEKHVSLHWTSDPLSDMV 480
           +NAFNVIVRRLKQVYESVESSTD+ESGVP +RVHDRVTVK ESE+HVSLHWTSDPLSDMV
Sbjct: 527 TNAFNVIVRRLKQVYESVESSTDEESGVPTVRVHDRVTVKHESERHVSLHWTSDPLSDMV 586

Query: 481 SDSVVALILNINREVPKVIVESEAVKTEEENVKKAEKVIHALLVSLFGDVKLGENGKLVI 540
           SDSVVALILNINREVPKVIVESEAVKTEEEN KKAEKVIHALLVSLFGDVKLGENGKLV+
Sbjct: 587 SDSVVALILNINREVPKVIVESEAVKTEEENEKKAEKVIHALLVSLFGDVKLGENGKLVV 646

Query: 541 NVDGNIAEVDKQSGEVESDNEALKERVKTAFQRIQCAVNPIPLSS 585
           NVDG+IAEVDKQSGEVES+NEALKERVKTAF+RIQ AV PIPLS+
Sbjct: 647 NVDGSIAEVDKQSGEVESENEALKERVKTAFRRIQSAVKPIPLSA 691

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C9521.7e-28681.66Cleavage and polyadenylation specificity factor subunit 3-I OS=Arabidopsis thali... [more]
P791011.3e-16450.59Cleavage and polyadenylation specificity factor subunit 3 OS=Bos taurus OX=9913 ... [more]
Q9UKF61.7e-16450.59Cleavage and polyadenylation specificity factor subunit 3 OS=Homo sapiens OX=960... [more]
Q9QXK71.1e-16350.25Cleavage and polyadenylation specificity factor subunit 3 OS=Mus musculus OX=100... [more]
Q86A791.7e-15645.34Cleavage and polyadenylation specificity factor subunit 3 OS=Dictyostelium disco... [more]
Match NameE-valueIdentityDescription
XP_011655552.20.0100.00cleavage and polyadenylation specificity factor subunit 3-I-like [Cucumis sativu... [more]
KAE8648661.10.0100.00hypothetical protein Csa_008894, partial [Cucumis sativus][more]
XP_008465568.20.099.32PREDICTED: cleavage and polyadenylation specificity factor subunit 3-I isoform X... [more]
KAA0034167.10.099.32cleavage and polyadenylation specificity factor subunit 3-I-like [Cucumis melo v... [more]
XP_011655548.20.099.15cleavage and polyadenylation specificity factor subunit 3-I isoform X1 [Cucumis ... [more]
Match NameE-valueIdentityDescription
A0A1S4E5B20.099.32cleavage and polyadenylation specificity factor subunit 3-I isoform X1 OS=Cucumi... [more]
A0A5A7SY150.099.32Cleavage and polyadenylation specificity factor subunit 3-I-like OS=Cucumis melo... [more]
A0A0A0KV820.099.15Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G587170 PE=4 SV=1[more]
A0A6J1DE610.096.25cleavage and polyadenylation specificity factor subunit 3-I OS=Momordica charant... [more]
A0A6J1G1N40.095.90cleavage and polyadenylation specificity factor subunit 3-I-like OS=Cucurbita mo... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 652..672
NoneNo IPR availableGENE3D3.40.50.10890coord: 226..405
e-value: 2.6E-151
score: 505.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
NoneNo IPR availablePANTHERPTHR11203CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR FAMILY MEMBERcoord: 7..689
NoneNo IPR availablePANTHERPTHR11203:SF48SUBFAMILY NOT NAMEDcoord: 7..689
NoneNo IPR availableCDDcd16292CPSF3-like_MBL-foldcoord: 24..217
e-value: 4.1599E-143
score: 413.905
IPR001279Metallo-beta-lactamaseSMARTSM00849Lactamase_B_5acoord: 36..224
e-value: 1.0E-12
score: 58.3
IPR001279Metallo-beta-lactamasePFAMPF00753Lactamase_Bcoord: 35..200
e-value: 6.8E-21
score: 75.2
IPR022712Beta-Casp domainSMARTSM01027Beta_Casp_2coord: 258..377
e-value: 2.1E-37
score: 140.3
IPR022712Beta-Casp domainPFAMPF10996Beta-Caspcoord: 258..377
e-value: 3.0E-26
score: 91.9
IPR021718Pre-mRNA 3'-end-processing endonuclease polyadenylation factor C-termSMARTSM01098CPSF73_100_C_2coord: 484..687
e-value: 9.0E-44
score: 161.4
IPR021718Pre-mRNA 3'-end-processing endonuclease polyadenylation factor C-termPFAMPF11718CPSF73-100_Ccoord: 487..686
e-value: 1.3E-45
score: 155.7
IPR011108Zn-dependent metallo-hydrolase, RNA specificity domainPFAMPF07521RMMBLcoord: 392..446
e-value: 3.6E-15
score: 55.7
IPR036866Ribonuclease Z/Hydroxyacylglutathione hydrolase-likeGENE3D3.60.15.10coord: 29..434
e-value: 2.6E-151
score: 505.7
IPR036866Ribonuclease Z/Hydroxyacylglutathione hydrolase-likeSUPERFAMILY56281Metallo-hydrolase/oxidoreductasecoord: 22..465

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G10004.T1Cucsat.G10004.T1mRNA
Cucsat.G10004.T2Cucsat.G10004.T2mRNA
Cucsat.G10004.T3Cucsat.G10004.T3mRNA
Cucsat.G10004.T5Cucsat.G10004.T5mRNA
Cucsat.G10004.T9Cucsat.G10004.T9mRNA
Cucsat.G10004.T6Cucsat.G10004.T6mRNA
Cucsat.G10004.T4Cucsat.G10004.T4mRNA
Cucsat.G10004.T7Cucsat.G10004.T7mRNA
Cucsat.G10004.T8Cucsat.G10004.T8mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006397 mRNA processing
cellular_component GO:0005634 nucleus