Csor.00g311170 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g311170
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Descriptionaspartic proteinase PCS1-like
LocationCsor_Chr15: 528528 .. 532470 (-)
RNA-Seq ExpressionCsor.00g311170
SyntenyCsor.00g311170
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCTTCTTCCTCCGCCTGCTGCAGCTTCTTATCTGCTGCGTCTCTTTCAAACAGGGCCTCTGTTTTTCTGCGACTCAGACCATGGTTTTGCCCCTCAAAACACAGATGGGTGTCACTTCTCGGCCTTCCAATAAGCTCAGTTTTCACCATAATGTCACTTTGACTGTTTCCTTAACGCTTGGCTCGCCTCCTCAACCCGTTACTATGGTTCTCGATACAGGGAGTGAACTCTCATGGCTTCACTGCAAAAAAACCCCAAATTTGAACTCTGTTTTTAACCCACTTTCTTCCTCTTCTTACTCGCCAGTCCCCTGTGCTTCCCCTGTTTGCCGGGCCCGAACCCGAGATTTACCCAACCCGGTTACCTGCGACCCAAAGAAACTCTGCCACGTCTTTGTCTCTTATGCCGACGCCTCGTCGCTCGAGGGCAATCTCGCGTCGGATACGTTTCGAGTCGGGTCATCGGCTCAACCCGGAACTTTTTTTGGGTGTATGGATTCGGGTTTCAGTTCGAATTCGGAGGAGGACGCGAAGACCACTGGGCTGATGGGGATGAACAGGGGCTCGCTCTCGTTTGTCACCCAATCGGGTTTGCCCAAATTCTCTTATTGCATATCGGGTCGTGATTCTTCTGGGGTTCTGCTTTTCGGCGACGCGAGTCTTTCTTGGCTTGGGAATTTGACCTACACGCCTTTGGTTCAAATGTCTACGCCATTGCCGTATTACGACCGAGTCGCCTACACGGTCCAACTAGACGGAATCAGAGTAGGGAACAAAATTCTGGCACTCCCGAAGTCAATATTCGCACCAGACCACACCGGCGCCGGGCAAACCATGGTAGATTCAGGGACCCAGTTCACGTTTCTTCTGGGGCCAGTGTACACGGCTTTAAAGAACGAGTTTGTGGTACAAACGAAGGGCATTTTGGTCCCACTGGGTGATCCAAATTTCGTGTTTCAAGGAGCGATGGACTTGTGCTACAGAGTACCCGAGAAACAGGGGAAACTGCCGCCACTGCCGGTAGTGAGTCTGATGTTCCGTGGGGCGGAGATGGTGGTTGGCGGAGAGGTGCTAATGTATAAAGTACCGGGAATGGTGAGGGGTGGTGACCAAGTGCATTGCTTGACGTTTGGGAATTCGGATTTGTTAGGAATAGAAGCATTTGTGATTGGGCATCATCATCAACAAAACGTGTGGATGGAATTCGACTTGGTGAAATCAAGGGTTGGATTTGTAGAGACGAGGTGTGATTTGGCGGGTGAGCGGCTGGGGCTGGGGCCTTAAAAAATTAAAAAAGAACTTTGAATTGAAAAATGGCCTGAAGCACTGCCCAATCCATGGCCCACCTCAAGAGGCGAGGAGGTGGCCCATTGATCGGACAGGCACGGGAACAGAGTCTCTCTCGGGTTTTATTTTAAAAAAAATTTTGATTGCTCTGTGAGATTCTCTGAAGAACATTGTGGAGATGTCAGGTCTTTTTGTTTTCGAAATGGTTCAAAATTTCTTTTCCATGTTTTGTTCATTGGGTTTGGTGTCGTGTTGGGTCATTCAGTTAGTACAATTTTGAATTGTATATATTTATATATATATATCTATATATTATTATATTATTGGTGTTTTCTGTCTTCAAGTTCTGACTTTTGATAAAGATAAAGGTTGCTAATTTTGCCAGCGCATCCTCTTAGCCCACCCCCACCTGCCGCCCCCTTTTGAGATTAAATCAATTTTTTGACAAAAAGAAAAAAGAAAAAAACAATAGGGTTTTTGTGAGCCGTGTGAGTTAAATTGAAGATTTTTAAATATTCATAGTAAATTAAAATATATTTTTTTAATAAAAAAGTATATTTGTGGAATTAAGAAAAAAAACTATTCAAAGAAACATGGACACTATGGAGTAGTATCACGTGAGATGAAAATCACGAGGGGGGCAGTACACACGTGTGGTTTGTTTTTAAGGAAAGATGCAAGTTGCCGACCAACCAGAGTCAATCAATTTGACACGTGTCATAGAATAAGGCGTGGACTCTATACACCTCAACCACAAAGGCCTAACTGCCTTGTTTATTGATTTTTGTCGTTTCTATTATATTTTTTATATTTCACAAATAAACTAACCCTTTCATTTTATTAATTGTATTAGATAAATACAGAAGCAAGATATAAAATTTAAAAATTTGTTATATAAAATATTATTATATATTTCTTTAATATGTTTTTGAAAACCTATTTTATAACCGATACATTTTAAAATTGTGAGATTGATGATAAACATAACTGGTCAAAGCGGATAATATCTATTAGCAGTGGTTTCGGAACTGTTACAAATGGTATCAGAGTTAGTCACATGATGGTGCGAGACAATAGTAGGAATAGGGCTAGACCCTCTCTATAATAGATGCATTTTAAATTCGTAAGAGCTGATAACAATACGTAATGAGCTAAAACGAATAATATTTACTAATGGATGCATTTGAAGCGAAGACGCTGGCTCCCAAAATGTAGAAACCTCTCTCTCTAATAGATGCATTTGAAGTATTATTTGGACTTATTTTTGATGTCCTCCAACAAACAAATGAATAATTTAACGTAATGGATAAGACCATTATTATTATTATTAAATTTTTAAAAGATAATATGGTTAAATCTTAAAAATGGAAATAGTTTCAAAATCATAGGTAGATATAAAATTTGCATTAATAAAGTTTGAAATTAGAATTAGGGCCATAGAATGGATCGGGTCGGTTGGTTTTTGGAAGGGTATGGGCCATGGGTCGTTCGGTAACGCTCGGGAAAAGTCTAGAGATTAGAAGAAGCAAAAGCAGGCGTTAGAACATAGAATTTGACCAAATCAAAATACTACGTCGTTTTGCGCATTCAACTCCATCTCCCAAAGCCTACCTTACTAGGAAGCGAAGCCAAGCGAAACACGGGTCACATAGCAACTCAACGCGTCCGAGTCACTTCCCTCACTAGACTCGCTCCTCCATCTTCTCCGTCGCCACATACGGGTTTTCATTCTCACATAATTTTAAGATACCCCATCGCCAAAACTGTCCGATTCCAAGCAAACTCTGTCGCTTTTGGACCAACACAATCACCCCTATTTACTCGATTCTGTTCTGTAAGTCTCGGATTTCCCTCACGAAGGATTATTCTGGTTTGATTATTTGCTGATCCCTGTTCTCTTGTGCCTTAATTTCTTGGGATTCGGTTTCTAGGGTTCCTTAAATGAGTGATTTTTATTTTTATCGTTTGAATTTCACTTGTTCAACTGTCATCATAGCTTACGGGGCTGTAAAATTGTTTCGTCTGTCTGATTCCATTCATGTTTTCGCTACATTTGACTATCTACGGGAGTTGGCTACGAATGTCGGGAATTGAGAATTTAGATGAAAAGAATTCAAAGCTGATCTGGTTCAACCGACCGATAGTTCTGATTTCGAAGTTGAGCATTTGCATTTTAGTGCAGACCTCAATCATGTCAATATTGGATGTCTTTATGCACTTCAGTTTGTGTGCAGTCATCTTTCGGTCGTTTGGTGTTCATTAGATGGCCATATGAGGGGGAATGTCACTGGATGTCTTTCCATACAATGCTGATTCATTCCTTAGAGGATATTCAGGATTGATATATTGTCTAAAGGAATCAATAAATTTGAGTTATCTAGTCTTTATGGAGCAGATGATTAGCTCATGAATGTGATTTATGCATTGAATTTGACAACTAGGAAACAATTAAGAACATGTCAATGCTTACGGATAGACCATTCTCATTGGGGCTGAGAATGATGAATGATTGGTTATGTAGTAAGGCGATGCGGGCATCATTTGATTCATTTTGTTTGTCTATTAAGATACTGGAGCCTCTTTCTTTATCAGTTCTGCAGATAATTATCATGAGTTCATCTGAATTTCATCCTGTAGATTCTCATTTCTCTGA

mRNA sequence

ATGGCCTTCTTCCTCCGCCTGCTGCAGCTTCTTATCTGCTGCGTCTCTTTCAAACAGGGCCTCTGTTTTTCTGCGACTCAGACCATGGTTTTGCCCCTCAAAACACAGATGGGTGTCACTTCTCGGCCTTCCAATAAGCTCAGTTTTCACCATAATGTCACTTTGACTGTTTCCTTAACGCTTGGCTCGCCTCCTCAACCCGTTACTATGGTTCTCGATACAGGGAGTGAACTCTCATGGCTTCACTGCAAAAAAACCCCAAATTTGAACTCTGTTTTTAACCCACTTTCTTCCTCTTCTTACTCGCCAGTCCCCTGTGCTTCCCCTGTTTGCCGGGCCCGAACCCGAGATTTACCCAACCCGGTTACCTGCGACCCAAAGAAACTCTGCCACGTCTTTGTCTCTTATGCCGACGCCTCGTCGCTCGAGGGCAATCTCGCGTCGGATACGTTTCGAGTCGGGTCATCGGCTCAACCCGGAACTTTTTTTGGGTGTATGGATTCGGGTTTCAGTTCGAATTCGGAGGAGGACGCGAAGACCACTGGGCTGATGGGGATGAACAGGGGCTCGCTCTCGTTTGTCACCCAATCGGGTTTGCCCAAATTCTCTTATTGCATATCGGGTCGTGATTCTTCTGGGGTTCTGCTTTTCGGCGACGCGAGTCTTTCTTGGCTTGGGAATTTGACCTACACGCCTTTGGTTCAAATGTCTACGCCATTGCCGTATTACGACCGAGTCGCCTACACGGTCCAACTAGACGGAATCAGAGTAGGGAACAAAATTCTGGCACTCCCGAAGTCAATATTCGCACCAGACCACACCGGCGCCGGGCAAACCATGGTAGATTCAGGGACCCAGTTCACGTTTCTTCTGGGGCCAGTGTACACGGCTTTAAAGAACGAGTTTGTGGTACAAACGAAGGGCATTTTGGTCCCACTGGGTGATCCAAATTTCGTGTTTCAAGGAGCGATGGACTTGTGCTACAGAGTACCCGAGAAACAGGGGAAACTGCCGCCACTGCCGGTAGTGAGTCTGATGTTCCGTGGGGCGGAGATGGTGGTTGGCGGAGAGGTGCTAATGTATAAAGTACCGGGAATGGTGAGGGGTGGTGACCAAGTGCATTGCTTGACGTTTGGGAATTCGGATTTGTTAGGAATAGAAGCATTTGTGATTGGGCATCATCATCAACAAAACGTGTGGATGGAATTCGACTTGGTGAAATCAAGGGTTGGATTTGTAGAGACGAGGTGTGATTTGGCGGATACCCCATCGCCAAAACTGTCCGATTCCAAGCAAACTCTGTCGCTTTTGGACCAACACAATCACCCCTATTTACTCGATTCTGTTCTATTCTCATTTCTCTGA

Coding sequence (CDS)

ATGGCCTTCTTCCTCCGCCTGCTGCAGCTTCTTATCTGCTGCGTCTCTTTCAAACAGGGCCTCTGTTTTTCTGCGACTCAGACCATGGTTTTGCCCCTCAAAACACAGATGGGTGTCACTTCTCGGCCTTCCAATAAGCTCAGTTTTCACCATAATGTCACTTTGACTGTTTCCTTAACGCTTGGCTCGCCTCCTCAACCCGTTACTATGGTTCTCGATACAGGGAGTGAACTCTCATGGCTTCACTGCAAAAAAACCCCAAATTTGAACTCTGTTTTTAACCCACTTTCTTCCTCTTCTTACTCGCCAGTCCCCTGTGCTTCCCCTGTTTGCCGGGCCCGAACCCGAGATTTACCCAACCCGGTTACCTGCGACCCAAAGAAACTCTGCCACGTCTTTGTCTCTTATGCCGACGCCTCGTCGCTCGAGGGCAATCTCGCGTCGGATACGTTTCGAGTCGGGTCATCGGCTCAACCCGGAACTTTTTTTGGGTGTATGGATTCGGGTTTCAGTTCGAATTCGGAGGAGGACGCGAAGACCACTGGGCTGATGGGGATGAACAGGGGCTCGCTCTCGTTTGTCACCCAATCGGGTTTGCCCAAATTCTCTTATTGCATATCGGGTCGTGATTCTTCTGGGGTTCTGCTTTTCGGCGACGCGAGTCTTTCTTGGCTTGGGAATTTGACCTACACGCCTTTGGTTCAAATGTCTACGCCATTGCCGTATTACGACCGAGTCGCCTACACGGTCCAACTAGACGGAATCAGAGTAGGGAACAAAATTCTGGCACTCCCGAAGTCAATATTCGCACCAGACCACACCGGCGCCGGGCAAACCATGGTAGATTCAGGGACCCAGTTCACGTTTCTTCTGGGGCCAGTGTACACGGCTTTAAAGAACGAGTTTGTGGTACAAACGAAGGGCATTTTGGTCCCACTGGGTGATCCAAATTTCGTGTTTCAAGGAGCGATGGACTTGTGCTACAGAGTACCCGAGAAACAGGGGAAACTGCCGCCACTGCCGGTAGTGAGTCTGATGTTCCGTGGGGCGGAGATGGTGGTTGGCGGAGAGGTGCTAATGTATAAAGTACCGGGAATGGTGAGGGGTGGTGACCAAGTGCATTGCTTGACGTTTGGGAATTCGGATTTGTTAGGAATAGAAGCATTTGTGATTGGGCATCATCATCAACAAAACGTGTGGATGGAATTCGACTTGGTGAAATCAAGGGTTGGATTTGTAGAGACGAGGTGTGATTTGGCGGATACCCCATCGCCAAAACTGTCCGATTCCAAGCAAACTCTGTCGCTTTTGGACCAACACAATCACCCCTATTTACTCGATTCTGTTCTATTCTCATTTCTCTGA

Protein sequence

MAFFLRLLQLLICCVSFKQGLCFSATQTMVLPLKTQMGVTSRPSNKLSFHHNVTLTVSLTLGSPPQPVTMVLDTGSELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCRARTRDLPNPVTCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKTTGLMGMNRGSLSFVTQSGLPKFSYCISGRDSSGVLLFGDASLSWLGNLTYTPLVQMSTPLPYYDRVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKNEFVVQTKGILVPLGDPNFVFQGAMDLCYRVPEKQGKLPPLPVVSLMFRGAEMVVGGEVLMYKVPGMVRGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLADTPSPKLSDSKQTLSLLDQHNHPYLLDSVLFSFL
Homology
BLAST of Csor.00g311170 vs. ExPASy Swiss-Prot
Match: Q9LZL3 (Aspartic proteinase PCS1 OS=Arabidopsis thaliana OX=3702 GN=PCS1 PE=2 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 1.0e-145
Identity = 252/413 (61.02%), Postives = 313/413 (75.79%), Query Frame = 0

Query: 16  SFKQGLCFSATQTMVLPLKTQMGVTS-RPSNKLSFHHNVTLTVSLTLGSPPQPVTMVLDT 75
           SF      S++QT+VLPLKT++  T  RP++KL FHHNVTLTV+LT+G+PPQ ++MV+DT
Sbjct: 33  SFSSFSSSSSSQTLVLPLKTRITPTDHRPTDKLHFHHNVTLTVTLTVGTPPQNISMVIDT 92

Query: 76  GSELSWLHCKKTPNLNSV--FNPLSSSSYSPVPCASPVCRARTRDLPNPVTCDPKKLCHV 135
           GSELSWL C ++ N N V  F+P  SSSYSP+PC+SP CR RTRD   P +CD  KLCH 
Sbjct: 93  GSELSWLRCNRSSNPNPVNNFDPTRSSSYSPIPCSSPTCRTRTRDFLIPASCDSDKLCHA 152

Query: 136 FVSYADASSLEGNLASDTFRVGSSAQPGT-FFGCMDSGFSSNSEEDAKTTGLMGMNRGSL 195
            +SYADASS EGNLA++ F  G+S       FGCM S   S+ EED KTTGL+GMNRGSL
Sbjct: 153 TLSYADASSSEGNLAAEIFHFGNSTNDSNLIFGCMGSVSGSDPEEDTKTTGLLGMNRGSL 212

Query: 196 SFVTQSGLPKFSYCISGRDS-SGVLLFGDASLSWLGNLTYTPLVQMSTPLPYYDRVAYTV 255
           SF++Q G PKFSYCISG D   G LL GD++ +WL  L YTPL+++STPLPY+DRVAYTV
Sbjct: 213 SFISQMGFPKFSYCISGTDDFPGFLLLGDSNFTWLTPLNYTPLIRISTPLPYFDRVAYTV 272

Query: 256 QLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKNEFVVQTKGIL 315
           QL GI+V  K+L +PKS+  PDHTGAGQTMVDSGTQFTFLLGPVYTAL++ F+ +T GIL
Sbjct: 273 QLTGIKVNGKLLPIPKSVLVPDHTGAGQTMVDSGTQFTFLLGPVYTALRSHFLNRTNGIL 332

Query: 316 VPLGDPNFVFQGAMDLCYRVPE---KQGKLPPLPVVSLMFRGAEMVVGGEVLMYKVPGMV 375
               DP+FVFQG MDLCYR+     + G L  LP VSL+F GAE+ V G+ L+Y+VP + 
Sbjct: 333 TVYEDPDFVFQGTMDLCYRISPVRIRSGILHRLPTVSLVFEGAEIAVSGQPLLYRVPHLT 392

Query: 376 RGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA 421
            G D V+C TFGNSDL+G+EA+VIGHHHQQN+W+EFDL +SR+G     CD++
Sbjct: 393 VGNDSVYCFTFGNSDLMGMEAYVIGHHHQQNMWIEFDLQRSRIGLAPVECDVS 445

BLAST of Csor.00g311170 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 4.1e-38
Identity = 114/367 (31.06%), Postives = 173/367 (47.14%), Query Frame = 0

Query: 57  VSLTLGSPPQPVTMVLDTGSELSWLHCKKTPNLNS----VFNPLSSSSYSPVPCASPVCR 116
           +++ +G+P    + ++DTGS+L W  C+      S    +FNP  SSS+S +PC S  C 
Sbjct: 98  MNVAIGTPDSSFSAIMDTGSDLIWTQCEPCTQCFSQPTPIFNPQDSSSFSTLPCESQYC- 157

Query: 117 ARTRDLPNPVTCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSS 176
              +DLP+  TC+  + C     Y D S+ +G +A++TF   +S+ P   FGC   G  +
Sbjct: 158 ---QDLPSE-TCNNNE-CQYTYGYGDGSTTQGYMATETFTFETSSVPNIAFGC---GEDN 217

Query: 177 NSEEDAKTTGLMGMNRGSLSFVTQSGLPKFSYCIS--GRDSSGVLLFGDASLSWLGNLTY 236
                    GL+GM  G LS  +Q G+ +FSYC++  G  S   L  G A+         
Sbjct: 218 QGFGQGNGAGLIGMGWGPLSLPSQLGVGQFSYCMTSYGSSSPSTLALGSAASGVPEGSPS 277

Query: 237 TPLVQMSTPLPYYDRVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFL 296
           T L+  S    Y     Y + L GI VG   L +P S F     G G  ++DSGT  T+L
Sbjct: 278 TTLIHSSLNPTY-----YYITLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYL 337

Query: 297 LGPVYTALKNEFVVQTKGILVPLGDPNFVFQGAMDLCYRVPEKQGKLPPLPVVSLMFRGA 356
               Y A+   F   T  I +P  D +      +  C++ P   G    +P +S+ F G 
Sbjct: 338 PQDAYNAVAQAF---TDQINLPTVDES---SSGLSTCFQQP-SDGSTVQVPEISMQFDGG 397

Query: 357 EMVVGGEVLMYKVPGMVRGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRV 416
            + +G + +      ++   + V CL  G+S  LGI  F  G+  QQ   + +DL    V
Sbjct: 398 VLNLGEQNI------LISPAEGVICLAMGSSSQLGISIF--GNIQQQETQVLYDLQNLAV 435

Query: 417 GFVETRC 418
            FV T+C
Sbjct: 458 SFVPTQC 435

BLAST of Csor.00g311170 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 4.6e-37
Identity = 111/368 (30.16%), Postives = 182/368 (49.46%), Query Frame = 0

Query: 57  VSLTLGSPPQPVTMVLDTGSELSWLHCKKTPNL----NSVFNPLSSSSYSPVPCASPVCR 116
           ++L++G+P QP + ++DTGS+L W  C+           +FNP  SSS+S +PC+S +C+
Sbjct: 97  MNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPIFNPQGSSSFSTLPCSSQLCQ 156

Query: 117 ARTRDLPNPVTCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSS 176
           A    L +P TC     C     Y D S  +G++ ++T   GS + P   FGC   G ++
Sbjct: 157 A----LSSP-TCS-NNFCQYTYGYGDGSETQGSMGTETLTFGSVSIPNITFGC---GENN 216

Query: 177 NSEEDAKTTGLMGMNRGSLSFVTQSGLPKFSYCIS--GRDSSGVLLFGDASLSWLGNLTY 236
                    GL+GM RG LS  +Q  + KFSYC++  G  +   LL G  + S       
Sbjct: 217 QGFGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMTPIGSSTPSNLLLGSLANSVTAGSPN 276

Query: 237 TPLVQMSTPLPYYDRVAYTVQLDGIRVGNKILALPKSIFA-PDHTGAGQTMVDSGTQFTF 296
           T L+Q S+ +P +    Y + L+G+ VG+  L +  S FA   + G G  ++DSGT  T+
Sbjct: 277 TTLIQ-SSQIPTF----YYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTY 336

Query: 297 LLGPVYTALKNEFVVQTKGILVPLGDPNFVFQGAMDLCYRVPEKQGKLPPLPVVSLMFRG 356
            +   Y +++ EF+ Q   I +P+ + +       DLC++ P     L  +P   + F G
Sbjct: 337 FVNNAYQSVRQEFISQ---INLPVVNGS---SSGFDLCFQTPSDPSNL-QIPTFVMHFDG 396

Query: 357 AEMVVGGEVLMYKVPGMVRGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSR 416
            ++ +  E         +   + + CL  G+S   G+  F  G+  QQN+ + +D   S 
Sbjct: 397 GDLELPSENY------FISPSNGLICLAMGSSS-QGMSIF--GNIQQQNMLVVYDTGNSV 434

Query: 417 VGFVETRC 418
           V F   +C
Sbjct: 457 VSFASAQC 434

BLAST of Csor.00g311170 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 9.9e-32
Identity = 118/370 (31.89%), Postives = 174/370 (47.03%), Query Frame = 0

Query: 59  LTLGSPPQPVTMVLDTGSELSWLHCKKTPNLNS----VFNPLSSSSYSPVPCASPVCRAR 118
           L +G+P + V MVLDTGS++ WL C       S    +F+P  S +Y+ +PC+SP CR  
Sbjct: 146 LGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDPIFDPRKSKTYATIPCSSPHCRR- 205

Query: 119 TRDLPNPVTCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNS 178
              L +      +K C   VSY D S   G+ +++T     +   G   GC       N 
Sbjct: 206 ---LDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFRRNRVKGVALGC----GHDNE 265

Query: 179 EEDAKTTGLMGMNRGSLSFVTQSG---LPKFSYCISGRDSS---GVLLFGDASLSWLGNL 238
                  GL+G+ +G LSF  Q+G     KFSYC+  R +S     ++FG+A++S +   
Sbjct: 266 GLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVVFGNAAVSRIAR- 325

Query: 239 TYTPLVQMSTPLPYYDRVAYTVQLDGIRV-GNKILALPKSIFAPDHTGAGQTMVDSGTQF 298
            +TPL+      P  D   Y V L GI V G ++  +  S+F  D  G G  ++DSGT  
Sbjct: 326 -FTPLLSN----PKLD-TFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSV 385

Query: 299 TFLLGPVYTALKNEFVVQTKGILVPLGDPNFVFQGAMDLCYRVPEKQGKLPPLPVVSLMF 358
           T L+ P Y A+++ F V  K +      P+F      D C+ +         +P V L F
Sbjct: 386 TRLIRPAYIAMRDAFRVGAKTL---KRAPDF---SLFDTCFDLSNMNE--VKVPTVVLHF 445

Query: 359 RGAEMVVGGEVLMYKVPGMVRGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVK 418
           RGA+  V      Y +P    G     C  F  + + G+   +IG+  QQ   + +DL  
Sbjct: 446 RGAD--VSLPATNYLIPVDTNG---KFCFAFAGT-MGGLS--IIGNIQQQGFRVVYDLAS 484

BLAST of Csor.00g311170 vs. ExPASy Swiss-Prot
Match: Q6F4N5 (Aspartyl protease 25 OS=Oryza sativa subsp. japonica OX=39947 GN=AP25 PE=2 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 4.1e-30
Identity = 119/379 (31.40%), Postives = 177/379 (46.70%), Query Frame = 0

Query: 61  LGSPPQPVTMVLDTGSELSWLHCKK--TPNLNSVFNPLSSSSYSPVPCASPVC------- 120
           LGSP Q + + LDT ++ +W HC    T   +S+F P +SSSY+ +PC+S  C       
Sbjct: 85  LGSPSQQLLLALDTSADATWAHCSPCGTCPSSSLFAPANSSSYASLPCSSSWCPLFQGQA 144

Query: 121 ----RARTRDLPNPVTCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMD 180
               +      P P T      C     +ADA S +  LASDT R+G  A P   FGC+ 
Sbjct: 145 CPAPQGGGDAAPPPATL---PTCAFSKPFADA-SFQAALASDTLRLGKDAIPNYTFGCVS 204

Query: 181 SGFSSNSEEDAKTTGLMGMNRGSLSFVTQSGL---PKFSYCISGRDS---SGVLLFGDAS 240
           S   +    +    GL+G+ RG ++ ++Q+G      FSYC+    S   SG L  G A 
Sbjct: 205 S--VTGPTTNMPRQGLLGLGRGPMALLSQAGSLYNGVFSYCLPSYRSYYFSGSLRLG-AG 264

Query: 241 LSWLGNLTYTPLVQMSTPLPYYDRVAYTVQLDGIRVGNKILALPKSIFAPD-HTGAGQTM 300
                ++ YTP+++     P+   + Y V + G+ VG+  + +P   FA D  TGAG T+
Sbjct: 265 GGQPRSVRYTPMLRN----PHRSSL-YYVNVTGLSVGHAWVKVPAGSFAFDAATGAG-TV 324

Query: 301 VDSGTQFTFLLGPVYTALKNEFVVQTKGILVPLGDPNFVFQGAMDLCYRVPEKQGKLPPL 360
           VDSGT  T    PVY AL+ EF    + +  P G   +   GA D C+   E      P 
Sbjct: 325 VDSGTVITRWTAPVYAALREEF---RRQVAAPSG---YTSLGAFDTCFNTDEVAAGGAPA 384

Query: 361 PVVSLMFRGAEMVVGGEVLMYKVPGMVRGGDQVHCLTFGNS-DLLGIEAFVIGHHHQQNV 419
             V  M  G ++ +  E  +     +      + CL    +   +     VI +  QQN+
Sbjct: 385 VTVH-MDGGVDLALPMENTL-----IHSSATPLACLAMAEAPQNVNSVVNVIANLQQQNI 438

BLAST of Csor.00g311170 vs. NCBI nr
Match: KAG6578412.1 (Aspartic proteinase PCS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 918 bits (2373), Expect = 0.0
Identity = 454/454 (100.00%), Postives = 454/454 (100.00%), Query Frame = 0

Query: 1   MAFFLRLLQLLICCVSFKQGLCFSATQTMVLPLKTQMGVTSRPSNKLSFHHNVTLTVSLT 60
           MAFFLRLLQLLICCVSFKQGLCFSATQTMVLPLKTQMGVTSRPSNKLSFHHNVTLTVSLT
Sbjct: 1   MAFFLRLLQLLICCVSFKQGLCFSATQTMVLPLKTQMGVTSRPSNKLSFHHNVTLTVSLT 60

Query: 61  LGSPPQPVTMVLDTGSELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCRARTRDLPN 120
           LGSPPQPVTMVLDTGSELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCRARTRDLPN
Sbjct: 61  LGSPPQPVTMVLDTGSELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCRARTRDLPN 120

Query: 121 PVTCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKT 180
           PVTCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKT
Sbjct: 121 PVTCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKT 180

Query: 181 TGLMGMNRGSLSFVTQSGLPKFSYCISGRDSSGVLLFGDASLSWLGNLTYTPLVQMSTPL 240
           TGLMGMNRGSLSFVTQSGLPKFSYCISGRDSSGVLLFGDASLSWLGNLTYTPLVQMSTPL
Sbjct: 181 TGLMGMNRGSLSFVTQSGLPKFSYCISGRDSSGVLLFGDASLSWLGNLTYTPLVQMSTPL 240

Query: 241 PYYDRVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKN 300
           PYYDRVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKN
Sbjct: 241 PYYDRVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKN 300

Query: 301 EFVVQTKGILVPLGDPNFVFQGAMDLCYRVPEKQGKLPPLPVVSLMFRGAEMVVGGEVLM 360
           EFVVQTKGILVPLGDPNFVFQGAMDLCYRVPEKQGKLPPLPVVSLMFRGAEMVVGGEVLM
Sbjct: 301 EFVVQTKGILVPLGDPNFVFQGAMDLCYRVPEKQGKLPPLPVVSLMFRGAEMVVGGEVLM 360

Query: 361 YKVPGMVRGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA 420
           YKVPGMVRGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA
Sbjct: 361 YKVPGMVRGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA 420

Query: 421 DTPSPKLSDSKQTLSLLDQHNHPYLLDSVLFSFL 454
           DTPSPKLSDSKQTLSLLDQHNHPYLLDSVLFSFL
Sbjct: 421 DTPSPKLSDSKQTLSLLDQHNHPYLLDSVLFSFL 454

BLAST of Csor.00g311170 vs. NCBI nr
Match: KAG7015977.1 (Aspartic proteinase PCS1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 850 bits (2197), Expect = 1.12e-310
Identity = 419/420 (99.76%), Postives = 419/420 (99.76%), Query Frame = 0

Query: 1   MAFFLRLLQLLICCVSFKQGLCFSATQTMVLPLKTQMGVTSRPSNKLSFHHNVTLTVSLT 60
           M FFLRLLQLLICCVSFKQGLCFSATQTMVLPLKTQMGVTSRPSNKLSFHHNVTLTVSLT
Sbjct: 1   MPFFLRLLQLLICCVSFKQGLCFSATQTMVLPLKTQMGVTSRPSNKLSFHHNVTLTVSLT 60

Query: 61  LGSPPQPVTMVLDTGSELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCRARTRDLPN 120
           LGSPPQPVTMVLDTGSELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCRARTRDLPN
Sbjct: 61  LGSPPQPVTMVLDTGSELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCRARTRDLPN 120

Query: 121 PVTCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKT 180
           PVTCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKT
Sbjct: 121 PVTCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKT 180

Query: 181 TGLMGMNRGSLSFVTQSGLPKFSYCISGRDSSGVLLFGDASLSWLGNLTYTPLVQMSTPL 240
           TGLMGMNRGSLSFVTQSGLPKFSYCISGRDSSGVLLFGDASLSWLGNLTYTPLVQMSTPL
Sbjct: 181 TGLMGMNRGSLSFVTQSGLPKFSYCISGRDSSGVLLFGDASLSWLGNLTYTPLVQMSTPL 240

Query: 241 PYYDRVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKN 300
           PYYDRVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKN
Sbjct: 241 PYYDRVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKN 300

Query: 301 EFVVQTKGILVPLGDPNFVFQGAMDLCYRVPEKQGKLPPLPVVSLMFRGAEMVVGGEVLM 360
           EFVVQTKGILVPLGDPNFVFQGAMDLCYRVPEKQGKLPPLPVVSLMFRGAEMVVGGEVLM
Sbjct: 301 EFVVQTKGILVPLGDPNFVFQGAMDLCYRVPEKQGKLPPLPVVSLMFRGAEMVVGGEVLM 360

Query: 361 YKVPGMVRGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA 420
           YKVPGMVRGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA
Sbjct: 361 YKVPGMVRGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA 420

BLAST of Csor.00g311170 vs. NCBI nr
Match: XP_022938661.1 (aspartic proteinase PCS1-like [Cucurbita moschata])

HSP 1 Score: 848 bits (2192), Expect = 6.46e-310
Identity = 418/420 (99.52%), Postives = 418/420 (99.52%), Query Frame = 0

Query: 1   MAFFLRLLQLLICCVSFKQGLCFSATQTMVLPLKTQMGVTSRPSNKLSFHHNVTLTVSLT 60
           MAFFLRLLQLLICCVSFKQGLCFSATQTMVLPLKTQMGVTSRPSNKLSFHHNVTLTVSLT
Sbjct: 1   MAFFLRLLQLLICCVSFKQGLCFSATQTMVLPLKTQMGVTSRPSNKLSFHHNVTLTVSLT 60

Query: 61  LGSPPQPVTMVLDTGSELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCRARTRDLPN 120
           LGSPPQPVTMVLDTGSELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCR RTRDLPN
Sbjct: 61  LGSPPQPVTMVLDTGSELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCRTRTRDLPN 120

Query: 121 PVTCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKT 180
           PVTCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKT
Sbjct: 121 PVTCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKT 180

Query: 181 TGLMGMNRGSLSFVTQSGLPKFSYCISGRDSSGVLLFGDASLSWLGNLTYTPLVQMSTPL 240
           TGLMGMNRGSLSFVTQ GLPKFSYCISGRDSSGVLLFGDASLSWLGNLTYTPLVQMSTPL
Sbjct: 181 TGLMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDASLSWLGNLTYTPLVQMSTPL 240

Query: 241 PYYDRVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKN 300
           PYYDRVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKN
Sbjct: 241 PYYDRVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKN 300

Query: 301 EFVVQTKGILVPLGDPNFVFQGAMDLCYRVPEKQGKLPPLPVVSLMFRGAEMVVGGEVLM 360
           EFVVQTKGILVPLGDPNFVFQGAMDLCYRVPEKQGKLPPLPVVSLMFRGAEMVVGGEVLM
Sbjct: 301 EFVVQTKGILVPLGDPNFVFQGAMDLCYRVPEKQGKLPPLPVVSLMFRGAEMVVGGEVLM 360

Query: 361 YKVPGMVRGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA 420
           YKVPGMVRGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA
Sbjct: 361 YKVPGMVRGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA 420

BLAST of Csor.00g311170 vs. NCBI nr
Match: XP_023549570.1 (aspartic proteinase PCS1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 842 bits (2175), Expect = 2.52e-307
Identity = 414/420 (98.57%), Postives = 415/420 (98.81%), Query Frame = 0

Query: 1   MAFFLRLLQLLICCVSFKQGLCFSATQTMVLPLKTQMGVTSRPSNKLSFHHNVTLTVSLT 60
           MAFFLRLLQLLICCVSFKQGLCFSATQTMVLPLKTQMGVTSRPSNKLSFHHNVTLTVSLT
Sbjct: 1   MAFFLRLLQLLICCVSFKQGLCFSATQTMVLPLKTQMGVTSRPSNKLSFHHNVTLTVSLT 60

Query: 61  LGSPPQPVTMVLDTGSELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCRARTRDLPN 120
           LGSPPQPVTMVLDTGSELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCR RTRDLPN
Sbjct: 61  LGSPPQPVTMVLDTGSELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCRTRTRDLPN 120

Query: 121 PVTCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKT 180
           PVTCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKT
Sbjct: 121 PVTCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKT 180

Query: 181 TGLMGMNRGSLSFVTQSGLPKFSYCISGRDSSGVLLFGDASLSWLGNLTYTPLVQMSTPL 240
           TGLMGMNRGSLSFVTQ GLPKFSYCISGRDSSGVLLFGD SLSWLGNLTYTPLVQMSTPL
Sbjct: 181 TGLMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDVSLSWLGNLTYTPLVQMSTPL 240

Query: 241 PYYDRVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKN 300
           PYYDRVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKN
Sbjct: 241 PYYDRVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKN 300

Query: 301 EFVVQTKGILVPLGDPNFVFQGAMDLCYRVPEKQGKLPPLPVVSLMFRGAEMVVGGEVLM 360
           EF+VQTKGILVPLGDPNFVFQGAMDLCY VPEKQGKLPPLPVVSLMFRGAEMVVGGEVLM
Sbjct: 301 EFLVQTKGILVPLGDPNFVFQGAMDLCYSVPEKQGKLPPLPVVSLMFRGAEMVVGGEVLM 360

Query: 361 YKVPGMVRGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA 420
           YKVPGMVRGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDL KSRVGFVETRCDLA
Sbjct: 361 YKVPGMVRGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLAKSRVGFVETRCDLA 420

BLAST of Csor.00g311170 vs. NCBI nr
Match: XP_022992613.1 (aspartic proteinase PCS1-like [Cucurbita maxima])

HSP 1 Score: 838 bits (2165), Expect = 8.41e-306
Identity = 412/420 (98.10%), Postives = 415/420 (98.81%), Query Frame = 0

Query: 1   MAFFLRLLQLLICCVSFKQGLCFSATQTMVLPLKTQMGVTSRPSNKLSFHHNVTLTVSLT 60
           MAFFLRLL LLICCVSFKQ LCFSA QTMVLPLKTQMGVTS+PSNKLSFHHNVTLTVSLT
Sbjct: 1   MAFFLRLLHLLICCVSFKQSLCFSAIQTMVLPLKTQMGVTSQPSNKLSFHHNVTLTVSLT 60

Query: 61  LGSPPQPVTMVLDTGSELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCRARTRDLPN 120
           LGSPPQPVTMVLDTGSELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCR RTRDLPN
Sbjct: 61  LGSPPQPVTMVLDTGSELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCRTRTRDLPN 120

Query: 121 PVTCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKT 180
           PVTCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKT
Sbjct: 121 PVTCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKT 180

Query: 181 TGLMGMNRGSLSFVTQSGLPKFSYCISGRDSSGVLLFGDASLSWLGNLTYTPLVQMSTPL 240
           TGLMGMNRGSLSFVTQ GLPKFSYCISGRDSSGVLLFGDASLSWLGNLTYTPLVQMSTPL
Sbjct: 181 TGLMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDASLSWLGNLTYTPLVQMSTPL 240

Query: 241 PYYDRVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKN 300
           PYYDRVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKN
Sbjct: 241 PYYDRVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKN 300

Query: 301 EFVVQTKGILVPLGDPNFVFQGAMDLCYRVPEKQGKLPPLPVVSLMFRGAEMVVGGEVLM 360
           EFVVQTKGILVPLGDPNFVFQGAMDLCYRVPEKQGKLPPLPVVSLMFRGAEMVVGGEVLM
Sbjct: 301 EFVVQTKGILVPLGDPNFVFQGAMDLCYRVPEKQGKLPPLPVVSLMFRGAEMVVGGEVLM 360

Query: 361 YKVPGMVRGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA 420
           Y+VPGMVRGGDQVHC+TFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA
Sbjct: 361 YRVPGMVRGGDQVHCVTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA 420

BLAST of Csor.00g311170 vs. ExPASy TrEMBL
Match: A0A6J1FDS6 (aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111444820 PE=3 SV=1)

HSP 1 Score: 848 bits (2192), Expect = 3.13e-310
Identity = 418/420 (99.52%), Postives = 418/420 (99.52%), Query Frame = 0

Query: 1   MAFFLRLLQLLICCVSFKQGLCFSATQTMVLPLKTQMGVTSRPSNKLSFHHNVTLTVSLT 60
           MAFFLRLLQLLICCVSFKQGLCFSATQTMVLPLKTQMGVTSRPSNKLSFHHNVTLTVSLT
Sbjct: 1   MAFFLRLLQLLICCVSFKQGLCFSATQTMVLPLKTQMGVTSRPSNKLSFHHNVTLTVSLT 60

Query: 61  LGSPPQPVTMVLDTGSELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCRARTRDLPN 120
           LGSPPQPVTMVLDTGSELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCR RTRDLPN
Sbjct: 61  LGSPPQPVTMVLDTGSELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCRTRTRDLPN 120

Query: 121 PVTCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKT 180
           PVTCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKT
Sbjct: 121 PVTCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKT 180

Query: 181 TGLMGMNRGSLSFVTQSGLPKFSYCISGRDSSGVLLFGDASLSWLGNLTYTPLVQMSTPL 240
           TGLMGMNRGSLSFVTQ GLPKFSYCISGRDSSGVLLFGDASLSWLGNLTYTPLVQMSTPL
Sbjct: 181 TGLMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDASLSWLGNLTYTPLVQMSTPL 240

Query: 241 PYYDRVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKN 300
           PYYDRVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKN
Sbjct: 241 PYYDRVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKN 300

Query: 301 EFVVQTKGILVPLGDPNFVFQGAMDLCYRVPEKQGKLPPLPVVSLMFRGAEMVVGGEVLM 360
           EFVVQTKGILVPLGDPNFVFQGAMDLCYRVPEKQGKLPPLPVVSLMFRGAEMVVGGEVLM
Sbjct: 301 EFVVQTKGILVPLGDPNFVFQGAMDLCYRVPEKQGKLPPLPVVSLMFRGAEMVVGGEVLM 360

Query: 361 YKVPGMVRGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA 420
           YKVPGMVRGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA
Sbjct: 361 YKVPGMVRGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA 420

BLAST of Csor.00g311170 vs. ExPASy TrEMBL
Match: A0A6J1JZR1 (aspartic proteinase PCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111488906 PE=3 SV=1)

HSP 1 Score: 838 bits (2165), Expect = 4.07e-306
Identity = 412/420 (98.10%), Postives = 415/420 (98.81%), Query Frame = 0

Query: 1   MAFFLRLLQLLICCVSFKQGLCFSATQTMVLPLKTQMGVTSRPSNKLSFHHNVTLTVSLT 60
           MAFFLRLL LLICCVSFKQ LCFSA QTMVLPLKTQMGVTS+PSNKLSFHHNVTLTVSLT
Sbjct: 1   MAFFLRLLHLLICCVSFKQSLCFSAIQTMVLPLKTQMGVTSQPSNKLSFHHNVTLTVSLT 60

Query: 61  LGSPPQPVTMVLDTGSELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCRARTRDLPN 120
           LGSPPQPVTMVLDTGSELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCR RTRDLPN
Sbjct: 61  LGSPPQPVTMVLDTGSELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCRTRTRDLPN 120

Query: 121 PVTCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKT 180
           PVTCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKT
Sbjct: 121 PVTCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKT 180

Query: 181 TGLMGMNRGSLSFVTQSGLPKFSYCISGRDSSGVLLFGDASLSWLGNLTYTPLVQMSTPL 240
           TGLMGMNRGSLSFVTQ GLPKFSYCISGRDSSGVLLFGDASLSWLGNLTYTPLVQMSTPL
Sbjct: 181 TGLMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDASLSWLGNLTYTPLVQMSTPL 240

Query: 241 PYYDRVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKN 300
           PYYDRVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKN
Sbjct: 241 PYYDRVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKN 300

Query: 301 EFVVQTKGILVPLGDPNFVFQGAMDLCYRVPEKQGKLPPLPVVSLMFRGAEMVVGGEVLM 360
           EFVVQTKGILVPLGDPNFVFQGAMDLCYRVPEKQGKLPPLPVVSLMFRGAEMVVGGEVLM
Sbjct: 301 EFVVQTKGILVPLGDPNFVFQGAMDLCYRVPEKQGKLPPLPVVSLMFRGAEMVVGGEVLM 360

Query: 361 YKVPGMVRGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA 420
           Y+VPGMVRGGDQVHC+TFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA
Sbjct: 361 YRVPGMVRGGDQVHCVTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA 420

BLAST of Csor.00g311170 vs. ExPASy TrEMBL
Match: A0A1S3B3Y8 (aspartic proteinase PCS1 OS=Cucumis melo OX=3656 GN=LOC103485512 PE=3 SV=1)

HSP 1 Score: 740 bits (1911), Expect = 1.95e-267
Identity = 363/411 (88.32%), Postives = 384/411 (93.43%), Query Frame = 0

Query: 11  LICCVSFKQGLCFSATQT-MVLPLKTQMGVTSRPSNKLSFHHNVTLTVSLTLGSPPQPVT 70
           L+  +S KQ LCFSAT T MVLPL+TQMG+ S+PSNKLSFHHNVTLTVSLT+GSPPQ VT
Sbjct: 10  LVVSLSMKQSLCFSATPTTMVLPLQTQMGLISQPSNKLSFHHNVTLTVSLTVGSPPQQVT 69

Query: 71  MVLDTGSELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCRARTRDLPNPVTCDPKKL 130
           MVLDTGSELSWLHCKK+PNL SVFNPLSSSSYSP+PC+SPVCR RTRDLPNPVTCDPKKL
Sbjct: 70  MVLDTGSELSWLHCKKSPNLTSVFNPLSSSSYSPIPCSSPVCRTRTRDLPNPVTCDPKKL 129

Query: 131 CHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKTTGLMGMNRG 190
           CH  VSYADASSLEGNLASD FR+GSSA PGT FGCMDSGFSSNSEEDAKTTGLMGMNRG
Sbjct: 130 CHAIVSYADASSLEGNLASDNFRIGSSALPGTLFGCMDSGFSSNSEEDAKTTGLMGMNRG 189

Query: 191 SLSFVTQSGLPKFSYCISGRDSSGVLLFGDASLSWLGNLTYTPLVQMSTPLPYYDRVAYT 250
           SLSFVTQ GLPKFSYCISGRDSSGVLLFGD+ LSWLGNLTYTPLVQ+STPLPY+DRVAYT
Sbjct: 190 SLSFVTQLGLPKFSYCISGRDSSGVLLFGDSHLSWLGNLTYTPLVQISTPLPYFDRVAYT 249

Query: 251 VQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKNEFVVQTKGI 310
           VQLDGIRVGNKIL LPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTAL+NEF+ QTKG+
Sbjct: 250 VQLDGIRVGNKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALRNEFLEQTKGV 309

Query: 311 LVPLGDPNFVFQGAMDLCYRVPEKQGKLPPLPVVSLMFRGAEMVVGGEVLMYKVPGMVRG 370
           L PLGDPNFVFQGAMDLCYRVP   GKLP LP VSLMFRGAEMVVGGEVL+YKVPGM++G
Sbjct: 310 LAPLGDPNFVFQGAMDLCYRVPAG-GKLPELPAVSLMFRGAEMVVGGEVLLYKVPGMMKG 369

Query: 371 GDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA 420
            + V+CLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA
Sbjct: 370 KEWVYCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA 419

BLAST of Csor.00g311170 vs. ExPASy TrEMBL
Match: A0A5D3DLY6 (Aspartic proteinase PCS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold205G00670 PE=3 SV=1)

HSP 1 Score: 738 bits (1906), Expect = 6.42e-267
Identity = 361/404 (89.36%), Postives = 380/404 (94.06%), Query Frame = 0

Query: 18  KQGLCFSATQT-MVLPLKTQMGVTSRPSNKLSFHHNVTLTVSLTLGSPPQPVTMVLDTGS 77
           KQ LCFSAT T MVLPL+TQMG+ S+PSNKLSFHHNVTLTVSLT+GSPPQ VTMVLDTGS
Sbjct: 2   KQSLCFSATPTTMVLPLQTQMGLISQPSNKLSFHHNVTLTVSLTVGSPPQQVTMVLDTGS 61

Query: 78  ELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCRARTRDLPNPVTCDPKKLCHVFVSY 137
           ELSWLHCKK+PNL SVFNPLSSSSYSP+PC+SPVCR RTRDLPNPVTCDPKKLCH  VSY
Sbjct: 62  ELSWLHCKKSPNLTSVFNPLSSSSYSPIPCSSPVCRTRTRDLPNPVTCDPKKLCHAIVSY 121

Query: 138 ADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKTTGLMGMNRGSLSFVTQ 197
           ADASSLEGNLASD FR+GSSA PGT FGCMDSGFSSNSEEDAKTTGLMGMNRGSLSFVTQ
Sbjct: 122 ADASSLEGNLASDNFRIGSSALPGTLFGCMDSGFSSNSEEDAKTTGLMGMNRGSLSFVTQ 181

Query: 198 SGLPKFSYCISGRDSSGVLLFGDASLSWLGNLTYTPLVQMSTPLPYYDRVAYTVQLDGIR 257
            GLPKFSYCISGRDSSGVLLFGD+ LSWLGNLTYTPLVQ+STPLPY+DRVAYTVQLDGIR
Sbjct: 182 LGLPKFSYCISGRDSSGVLLFGDSHLSWLGNLTYTPLVQISTPLPYFDRVAYTVQLDGIR 241

Query: 258 VGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKNEFVVQTKGILVPLGDP 317
           VGNKIL LPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTAL+NEF+ QTKG+L PLGDP
Sbjct: 242 VGNKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALRNEFLEQTKGVLAPLGDP 301

Query: 318 NFVFQGAMDLCYRVPEKQGKLPPLPVVSLMFRGAEMVVGGEVLMYKVPGMVRGGDQVHCL 377
           NFVFQGAMDLCYRVP   GKLP LP VSLMFRGAEMVVGGEVL+YKVPGM++G + V+CL
Sbjct: 302 NFVFQGAMDLCYRVPAG-GKLPELPAVSLMFRGAEMVVGGEVLLYKVPGMMKGKEWVYCL 361

Query: 378 TFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA 420
           TFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA
Sbjct: 362 TFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA 404

BLAST of Csor.00g311170 vs. ExPASy TrEMBL
Match: E5GC71 (Aspartic proteinase nepenthesin-1 OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 738 bits (1906), Expect = 6.42e-267
Identity = 361/404 (89.36%), Postives = 380/404 (94.06%), Query Frame = 0

Query: 18  KQGLCFSATQT-MVLPLKTQMGVTSRPSNKLSFHHNVTLTVSLTLGSPPQPVTMVLDTGS 77
           KQ LCFSAT T MVLPL+TQMG+ S+PSNKLSFHHNVTLTVSLT+GSPPQ VTMVLDTGS
Sbjct: 2   KQSLCFSATPTTMVLPLQTQMGLISQPSNKLSFHHNVTLTVSLTVGSPPQQVTMVLDTGS 61

Query: 78  ELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCRARTRDLPNPVTCDPKKLCHVFVSY 137
           ELSWLHCKK+PNL SVFNPLSSSSYSP+PC+SPVCR RTRDLPNPVTCDPKKLCH  VSY
Sbjct: 62  ELSWLHCKKSPNLTSVFNPLSSSSYSPIPCSSPVCRTRTRDLPNPVTCDPKKLCHAIVSY 121

Query: 138 ADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKTTGLMGMNRGSLSFVTQ 197
           ADASSLEGNLASD FR+GSSA PGT FGCMDSGFSSNSEEDAKTTGLMGMNRGSLSFVTQ
Sbjct: 122 ADASSLEGNLASDNFRIGSSALPGTLFGCMDSGFSSNSEEDAKTTGLMGMNRGSLSFVTQ 181

Query: 198 SGLPKFSYCISGRDSSGVLLFGDASLSWLGNLTYTPLVQMSTPLPYYDRVAYTVQLDGIR 257
            GLPKFSYCISGRDSSGVLLFGD+ LSWLGNLTYTPLVQ+STPLPY+DRVAYTVQLDGIR
Sbjct: 182 LGLPKFSYCISGRDSSGVLLFGDSHLSWLGNLTYTPLVQISTPLPYFDRVAYTVQLDGIR 241

Query: 258 VGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKNEFVVQTKGILVPLGDP 317
           VGNKIL LPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTAL+NEF+ QTKG+L PLGDP
Sbjct: 242 VGNKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALRNEFLEQTKGVLAPLGDP 301

Query: 318 NFVFQGAMDLCYRVPEKQGKLPPLPVVSLMFRGAEMVVGGEVLMYKVPGMVRGGDQVHCL 377
           NFVFQGAMDLCYRVP   GKLP LP VSLMFRGAEMVVGGEVL+YKVPGM++G + V+CL
Sbjct: 302 NFVFQGAMDLCYRVPAG-GKLPELPAVSLMFRGAEMVVGGEVLLYKVPGMMKGKEWVYCL 361

Query: 378 TFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA 420
           TFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA
Sbjct: 362 TFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA 404

BLAST of Csor.00g311170 vs. TAIR 10
Match: AT2G39710.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 585.9 bits (1509), Expect = 2.8e-167
Identity = 297/423 (70.21%), Postives = 340/423 (80.38%), Query Frame = 0

Query: 4   FLRLLQLLICCVSFKQGLC--FSATQTMVLPLKTQMGVTSRPSNKLSFHHNVTLTVSLTL 63
           FLR+  LL+    F    C   S  QT++  LKTQ  +    S+KLSF HNVTLTV+L +
Sbjct: 16  FLRISVLLLI---FPLTFCKTSSTNQTLLFSLKTQK-LPQSSSDKLSFRHNVTLTVTLAV 75

Query: 64  GSPPQPVTMVLDTGSELSWLHCKKTPNLNSVFNPLSSSSYSPVPCASPVCRARTRDLPNP 123
           G PPQ ++MVLDTGSELSWLHCKK+PNL SVFNP+SSS+YSPVPC+SP+CR RTRDLP P
Sbjct: 76  GDPPQNISMVLDTGSELSWLHCKKSPNLGSVFNPVSSSTYSPVPCSSPICRTRTRDLPIP 135

Query: 124 VTCDPK-KLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAKT 183
            +CDPK  LCHV +SYADA+S+EGNLA +TF +GS  +PGT FGCMDSG SSNSEEDAK+
Sbjct: 136 ASCDPKTHLCHVAISYADATSIEGNLAHETFVIGSVTRPGTLFGCMDSGLSSNSEEDAKS 195

Query: 184 TGLMGMNRGSLSFVTQSGLPKFSYCISGRDSSGVLLFGDASLSWLGNLTYTPLVQMSTPL 243
           TGLMGMNRGSLSFV Q G  KFSYCISG DSSG LL GDAS SWLG + YTPLV  STPL
Sbjct: 196 TGLMGMNRGSLSFVNQLGFSKFSYCISGSDSSGFLLLGDASYSWLGPIQYTPLVLQSTPL 255

Query: 244 PYYDRVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKN 303
           PY+DRVAYTVQL+GIRVG+KIL+LPKS+F PDHTGAGQTMVDSGTQFTFL+GPVYTALKN
Sbjct: 256 PYFDRVAYTVQLEGIRVGSKILSLPKSVFVPDHTGAGQTMVDSGTQFTFLMGPVYTALKN 315

Query: 304 EFVVQTKGILVPLGDPNFVFQGAMDLCYRV-PEKQGKLPPLPVVSLMFRGAEMVVGGEVL 363
           EF+ QTK +L  + DP+FVFQG MDLCY+V    +     LP+VSLMFRGAEM V G+ L
Sbjct: 316 EFITQTKSVLRLVDDPDFVFQGTMDLCYKVGSTTRPNFSGLPMVSLMFRGAEMSVSGQKL 375

Query: 364 MYKVPGM-VRGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFV-ETRC 421
           +Y+V G    G ++V+C TFGNSDLLGIEAFVIGHHHQQNVWMEFDL KSRVGF    RC
Sbjct: 376 LYRVNGAGSEGKEEVYCFTFGNSDLLGIEAFVIGHHHQQNVWMEFDLAKSRVGFAGNVRC 434

BLAST of Csor.00g311170 vs. TAIR 10
Match: AT5G02190.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 518.1 bits (1333), Expect = 7.3e-147
Identity = 252/413 (61.02%), Postives = 313/413 (75.79%), Query Frame = 0

Query: 16  SFKQGLCFSATQTMVLPLKTQMGVTS-RPSNKLSFHHNVTLTVSLTLGSPPQPVTMVLDT 75
           SF      S++QT+VLPLKT++  T  RP++KL FHHNVTLTV+LT+G+PPQ ++MV+DT
Sbjct: 33  SFSSFSSSSSSQTLVLPLKTRITPTDHRPTDKLHFHHNVTLTVTLTVGTPPQNISMVIDT 92

Query: 76  GSELSWLHCKKTPNLNSV--FNPLSSSSYSPVPCASPVCRARTRDLPNPVTCDPKKLCHV 135
           GSELSWL C ++ N N V  F+P  SSSYSP+PC+SP CR RTRD   P +CD  KLCH 
Sbjct: 93  GSELSWLRCNRSSNPNPVNNFDPTRSSSYSPIPCSSPTCRTRTRDFLIPASCDSDKLCHA 152

Query: 136 FVSYADASSLEGNLASDTFRVGSSAQPGT-FFGCMDSGFSSNSEEDAKTTGLMGMNRGSL 195
            +SYADASS EGNLA++ F  G+S       FGCM S   S+ EED KTTGL+GMNRGSL
Sbjct: 153 TLSYADASSSEGNLAAEIFHFGNSTNDSNLIFGCMGSVSGSDPEEDTKTTGLLGMNRGSL 212

Query: 196 SFVTQSGLPKFSYCISGRDS-SGVLLFGDASLSWLGNLTYTPLVQMSTPLPYYDRVAYTV 255
           SF++Q G PKFSYCISG D   G LL GD++ +WL  L YTPL+++STPLPY+DRVAYTV
Sbjct: 213 SFISQMGFPKFSYCISGTDDFPGFLLLGDSNFTWLTPLNYTPLIRISTPLPYFDRVAYTV 272

Query: 256 QLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKNEFVVQTKGIL 315
           QL GI+V  K+L +PKS+  PDHTGAGQTMVDSGTQFTFLLGPVYTAL++ F+ +T GIL
Sbjct: 273 QLTGIKVNGKLLPIPKSVLVPDHTGAGQTMVDSGTQFTFLLGPVYTALRSHFLNRTNGIL 332

Query: 316 VPLGDPNFVFQGAMDLCYRVPE---KQGKLPPLPVVSLMFRGAEMVVGGEVLMYKVPGMV 375
               DP+FVFQG MDLCYR+     + G L  LP VSL+F GAE+ V G+ L+Y+VP + 
Sbjct: 333 TVYEDPDFVFQGTMDLCYRISPVRIRSGILHRLPTVSLVFEGAEIAVSGQPLLYRVPHLT 392

Query: 376 RGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLA 421
            G D V+C TFGNSDL+G+EA+VIGHHHQQN+W+EFDL +SR+G     CD++
Sbjct: 393 VGNDSVYCFTFGNSDLMGMEAYVIGHHHQQNMWIEFDLQRSRIGLAPVECDVS 445

BLAST of Csor.00g311170 vs. TAIR 10
Match: AT5G37540.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 243.4 bits (620), Expect = 3.4e-64
Identity = 149/402 (37.06%), Postives = 215/402 (53.48%), Query Frame = 0

Query: 41  SRPSNKLSFHHNV----TLTVSLTLGSPPQPVTMVLDTGSELSWLHC------KKTPNLN 100
           S PS+  +F  N+     L +SL +G+P Q   +VLDTGS+LSW+ C      K  P   
Sbjct: 62  SPPSSPYTFRSNIKYSMALILSLPIGTPSQSQELVLDTGSQLSWIQCHPKKIKKPLPPPT 121

Query: 101 SVFNPLSSSSYSPVPCASPVCRARTRDLPNPVTCDPKKLCHVFVSYADASSLEGNLASDT 160
           + F+P  SSS+S +PC+ P+C+ R  D   P +CD  +LCH    YAD +  EGNL  + 
Sbjct: 122 TSFDPSLSSSFSDLPCSHPLCKPRIPDFTLPTSCDSNRLCHYSYFYADGTFAEGNLVKEK 181

Query: 161 FRVGSS-AQPGTFFGCMDSGFSSNSEEDAKTTGLMGMNRGSLSFVTQSGLPKFSYCISGR 220
           F   +S   P    GC        ++E     G++GMN G LSF++Q+ + KFSYCI  R
Sbjct: 182 FTFSNSQTTPPLILGC--------AKESTDEKGILGMNLGRLSFISQAKISKFSYCIPTR 241

Query: 221 D------SSGVLLFGD----ASLSWLGNLTYTPLVQMSTPLPYYDRVAYTVQLDGIRVGN 280
                  S+G    GD        ++  LT+      S  +P  D +AYTV L GIR+G 
Sbjct: 242 SNRPGLASTGSFYLGDNPNSRGFKYVSLLTF----PQSQRMPNLDPLAYTVPLQGIRIGQ 301

Query: 281 KILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKNEFVVQTKGILVPLGDPNFV 340
           K L +P S+F PD  G+GQTMVDSG++FT L+   Y  +K E +V+  G  +  G   +V
Sbjct: 302 KRLNIPGSVFRPDAGGSGQTMVDSGSEFTHLVDVAYDKVKEE-IVRLVGSRLKKG---YV 361

Query: 341 FQGAMDLCYRVPEKQ--GKLPPLPVVSLMFRGAEMVVGGEVLMYKVPGMVRGGDQVHCLT 400
           +    D+C+        G+L    +  L+F   E   G E+L+ K   +V  G  +HC+ 
Sbjct: 362 YGSTADMCFDGNHSMEIGRL----IGDLVF---EFGRGVEILVEKQSLLVNVGGGIHCVG 421

Query: 401 FGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDL 420
            G S +LG  + +IG+ HQQN+W+EFD+   RVGF +  C L
Sbjct: 422 IGRSSMLGAASNIIGNVHQQNLWVEFDVTNRRVGFSKAECRL 440

BLAST of Csor.00g311170 vs. TAIR 10
Match: AT1G66180.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 237.7 bits (605), Expect = 1.9e-62
Identity = 144/396 (36.36%), Postives = 211/396 (53.28%), Query Frame = 0

Query: 40  TSRPSN-KLSFHHNVTLTVSLTLGSPPQPVTMVLDTGSELSWLHC---KKTPNLNSVFNP 99
           +S P N +  F +++ L +SL +G+PPQ   MVLDTGS+LSW+ C   K  P   + F+P
Sbjct: 56  SSPPYNFRSRFKYSMALIISLPIGTPPQAQQMVLDTGSQLSWIQCHRKKLPPKPKTSFDP 115

Query: 100 LSSSSYSPVPCASPVCRARTRDLPNPVTCDPKKLCHVFVSYADASSLEGNLASDTFRVGS 159
             SSS+S +PC+ P+C+ R  D   P +CD  +LCH    YAD +  EGNL  +     +
Sbjct: 116 SLSSSFSTLPCSHPLCKPRIPDFTLPTSCDSNRLCHYSYFYADGTFAEGNLVKEKITFSN 175

Query: 160 S-AQPGTFFGCMDSGFSSNSEEDAKTTGLMGMNRGSLSFVTQSGLPKFSYCI------SG 219
           +   P    GC        + E +   G++GMNRG LSFV+Q+ + KFSYCI       G
Sbjct: 176 TEITPPLILGC--------ATESSDDRGILGMNRGRLSFVSQAKISKFSYCIPPKSNRPG 235

Query: 220 RDSSGVLLFGDASLSWLGNLTYTPLVQM--STPLPYYDRVAYTVQLDGIRVGNKILALPK 279
              +G    GD   S      Y  L+    S  +P  D +AYTV + GIR G K L +  
Sbjct: 236 FTPTGSFYLGDNPNS--HGFKYVSLLTFPESQRMPNLDPLAYTVPMIGIRFGLKKLNISG 295

Query: 280 SIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALKNEFVVQTKGILVPLGDPNFVFQGAMDL 339
           S+F PD  G+GQTMVDSG++FT L+   Y  ++ E + +    L       +V+ G  D+
Sbjct: 296 SVFRPDAGGSGQTMVDSGSEFTHLVDAAYDKVRAEIMTRVGRRL----KKGYVYGGTADM 355

Query: 340 CYRVPEKQGKLPPLP-----VVSLMFRGAEMVVGGEVLMYKVPGMVRGGDQVHCLTFGNS 399
           C+      G +  +P     +V +  RG E++V  E ++  V      G  +HC+  G S
Sbjct: 356 CF-----DGNVAMIPRLIGDLVFVFTRGVEILVPKERVLVNV------GGGIHCVGIGRS 415

Query: 400 DLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRC 418
            +LG  + +IG+ HQQN+W+EFD+   RVGF +  C
Sbjct: 416 SMLGAASNIIGNVHQQNLWVEFDVTNRRVGFAKADC 426

BLAST of Csor.00g311170 vs. TAIR 10
Match: AT2G03200.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 157.5 bits (397), Expect = 2.5e-38
Identity = 120/379 (31.66%), Postives = 184/379 (48.55%), Query Frame = 0

Query: 57  VSLTLGSPPQPVTMVLDTGSELSWLHCKKTPNL----NSVFNPLSSSSYSPVPCASPVCR 116
           + L++G+P    + ++DTGS+L W  CK           +F+P  SSSYS V C+S +C 
Sbjct: 109 MELSIGNPAVKYSAIVDTGSDLIWTQCKPCTECFDQPTPIFDPEKSSSYSKVGCSSGLCN 168

Query: 117 ARTRDLPNPVTCDPKKLCHVFVSYADASSLEGNLASDTFRV-GSSAQPGTFFGCMDSGFS 176
           A    LP     + K  C    +Y D SS  G LA++TF     ++  G  FGC   G  
Sbjct: 169 A----LPRSNCNEDKDACEYLYTYGDYSSTRGLLATETFTFEDENSISGIGFGC---GVE 228

Query: 177 SNSEEDAKTTGLMGMNRGSLSFVTQSGLPKFSYCIS-------------GRDSSGVLLFG 236
           +  +  ++ +GL+G+ RG LS ++Q    KFSYC++             G  +SG++   
Sbjct: 229 NEGDGFSQGSGLVGLGRGPLSLISQLKETKFSYCLTSIEDSEASSSLFIGSLASGIVNKT 288

Query: 237 DASLSWLGNLTYTPLVQMSTPLPYYDRVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQ 296
            ASL   G +T T  +  +   P +    Y ++L GI VG K L++ KS F     G G 
Sbjct: 289 GASLD--GEVTKTMSLLRNPDQPSF----YYLELQGITVGAKRLSVEKSTFELAEDGTGG 348

Query: 297 TMVDSGTQFTFLLGPVYTALKNEFVVQTKGILVPLGDPNFVFQGAMDLCYRVPEKQGKLP 356
            ++DSGT  T+L    +  LK EF   T  + +P+ D        +DLC+++P+   K  
Sbjct: 349 MIIDSGTTITYLEETAFKVLKEEF---TSRMSLPVDDSG---STGLDLCFKLPD-AAKNI 408

Query: 357 PLPVVSLMFRGAEMVVGGEVLMYKVPGMVRGGDQVHCLTFGNSDLLGIEAFVIGHHHQQN 416
            +P +   F+GA++ + GE  M     +      V CL  G+S+ + I     G+  QQN
Sbjct: 409 AVPKMIFHFKGADLELPGENYM-----VADSSTGVLCLAMGSSNGMSI----FGNVQQQN 458

Query: 417 VWMEFDLVKSRVGFVETRC 418
             +  DL K  V FV T C
Sbjct: 469 FNVLHDLEKETVSFVPTEC 458

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LZL31.0e-14561.02Aspartic proteinase PCS1 OS=Arabidopsis thaliana OX=3702 GN=PCS1 PE=2 SV=1[more]
Q766C24.1e-3831.06Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S... [more]
Q766C34.6e-3730.16Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S... [more]
Q9LNJ39.9e-3231.89Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ... [more]
Q6F4N54.1e-3031.40Aspartyl protease 25 OS=Oryza sativa subsp. japonica OX=39947 GN=AP25 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
KAG6578412.10.0100.00Aspartic proteinase PCS1, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7015977.11.12e-31099.76Aspartic proteinase PCS1, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022938661.16.46e-31099.52aspartic proteinase PCS1-like [Cucurbita moschata][more]
XP_023549570.12.52e-30798.57aspartic proteinase PCS1-like [Cucurbita pepo subsp. pepo][more]
XP_022992613.18.41e-30698.10aspartic proteinase PCS1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1FDS63.13e-31099.52aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111444820 PE=3... [more]
A0A6J1JZR14.07e-30698.10aspartic proteinase PCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111488906 PE=3 S... [more]
A0A1S3B3Y81.95e-26788.32aspartic proteinase PCS1 OS=Cucumis melo OX=3656 GN=LOC103485512 PE=3 SV=1[more]
A0A5D3DLY66.42e-26789.36Aspartic proteinase PCS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
E5GC716.42e-26789.36Aspartic proteinase nepenthesin-1 OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT2G39710.12.8e-16770.21Eukaryotic aspartyl protease family protein [more]
AT5G02190.17.3e-14761.02Eukaryotic aspartyl protease family protein [more]
AT5G37540.13.4e-6437.06Eukaryotic aspartyl protease family protein [more]
AT1G66180.11.9e-6236.36Eukaryotic aspartyl protease family protein [more]
AT2G03200.12.5e-3831.66Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 247..413
e-value: 1.6E-35
score: 122.3
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 223..422
e-value: 1.8E-42
score: 147.1
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 48..218
e-value: 5.7E-35
score: 123.0
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 55..418
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 57..219
e-value: 1.2E-38
score: 133.1
NoneNo IPR availablePANTHERPTHR47965:SF49EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEINcoord: 9..421
IPR001461Aspartic peptidase A1 familyPANTHERPTHR47965ASPARTYL PROTEASE-RELATEDcoord: 9..421
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 70..81
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 55..413
score: 33.938034
IPR034161Pepsin-like domain, plantCDDcd05476pepsin_A_like_plantcoord: 54..417
e-value: 1.57224E-69
score: 219.827

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g311170.m01Csor.00g311170.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004190 aspartic-type endopeptidase activity