Csor.00g305220 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g305220
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionDNA-directed primase/polymerase protein
LocationCsor_Chr18: 12771670 .. 12774481 (+)
RNA-Seq ExpressionCsor.00g305220
SyntenyCsor.00g305220
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codoninitialpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTTCAAGTGCGGCGTTTCTCATCCTTGTAAGTTCTGTTTCTCTAATTTTAATTCGAGTTTCTACAACCTAGGCAGCCATGCGAGCTCTGCGAAACAATGTACTGCGAATCTCGTTTTTAGACACAATGAATTTCACTGTGAAGCATTTGTAATTATTGTAGAAAAACTACTAGATTTTGTAAACTGGAATTACGATAGAGGAGCAATGTTATAGGTCGAGTTTCTGCTGTGATTTGGTGATCAGCATGGATGTTCTTTGTTTCTGTATTTTCTGCTTCTGATTATGTGCGGTTATCCATCCTGTTTCTGTTCAGAATCTTCCGTGAGAGGAAAAAAGAAAGAAAAATTACATTGAAGCCTGAGAGTTCAGCAAACTTTGAGACTCCAGTTTCTGATTCAGTTAAGGAAATACAGGACAATGCAACGAACGGCCAAGATTTCATGGAAAAAGTAAGTCGTTGTTTTCTGGATGGCGGAGTTTGCCTGAATTCCATGAACTTATTCTTCATATGCTTATTCAAGTATTTCAGCTGTCTAATGAAGTTCAGAGTTCGTTGAGCTAATGATGAGCCTGAAACAGCTTGATTATTGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCGTTGCTTGACAAAACTATAGTTATCACGATTCAGTTTGGTTTGACAAGTAAAGCAAAAGGTTCTAGTTATAAGAAGTTCTCGCCAGTCTTATTTTATAATTCACCTAGAGGCGTACCTCCGAGGAGGCCATCTAGTTTGTGGAGATTATTGCTTGAAATTCGTGTCGAGCTTTCTGAACAAAGTAGATTCAAGTTTAGGTATATTTAGGTTTCATCAAATCTCTCCCATTCCTCTTCCTGTTTCACCATCATTCCACTTCTCGATGATTCCATTTGACATTTCTGGTATCTTTTAAGTAGTGAAGTTTGCAATGAAGTTTGCTGTGAAGTTTGGACCACATTTCCTAGACAAGAAGAAGCAATGAAAGTTTGCAAGAGAGCACACAGATGTTCATATTTTAGTTATCAAGATCACTTTAATGAACAAAGGAGATTTCTAGTTTCATCACACAGCAAGTTTTGGCAATGTTGTCATCAAATTTTAATAATACTTCGAGTAGAAAAATTATTTTTCCATCAGCCTAGCAAACGCTTCTGATGTCGTTCTAACTACGTTCTTAATTAATTTCAGGTACAAAAGCATGGATTCAAAATTTAGCCACCATTAAATTGATTCAAGAGGTGAGCTGAAAAAGTTCGAGTCAATGTGCCGATCATGTAATTGAATCTGTGCGAATCGTGTTCTCTTTTCTTTTTTCTACTGCACTTTCTCACTGTTCTATTCCAACGTTCAGGTTGCTCCGCGTCATGTTTACTTTGATATGGAGTACAAGATAAAAATCAGCGCGGGAAAAATTGGAGACGTAATGGTTGATTCCTAGATATCAGTTGTTCTAGAGGCCTCAAATTGAAAGTATTCGATTCAAGGAAGCTTTGACTGGATACTAGAGCTTGATTCCTCAAACAAGGTATATTTAGCAACAATTGGATTTAGGCTTCATCTTCTTGTCACTTGTGTTATTTCTTTGTTATCTCTTGAAATGTATACTTAATAGGTGAAACTAGGTTCTTGTAATTTCTTTTCTTTTCTTTTATTTTTTAGACATCGCATAGCTCAAATTTTCTTGGTATTAGTATAACATATCATTTTTATCCTTTTTATTAGTAACAAGTTCATAATAGTTAACAGGATTATTTCTGGTAATTCTACAGGAATATGATTACTTCATTTCTTAAAGAAAATTATTTTTGCTAGACAAGTTCAGATTAAAAATATATATATAATTTTGGTTTCCAACAGAAAAGAGGAAGTTGAAGGCACTTCGTAGTCATCATTATTTAGAAATTTTGCCAAAACTTTTCCAATATCGGAGAAATTGTTTTACTCCACCTTTTAAGAAAGTAGTTGCGCAGGATACATTATTCTGAGAAGGATCCTATTATTTACCTTGTACATACTTAATCGTAGTGTGAGGAAGACATGTTCATGGCATCCTTGGTCTGCAACGTCGATGCTGAGTGTGAGAAACTTCTGGTTTGCGTAACGGAATTACACTGCGTAAAGACATTGCAATTTGAAATGGAGGTACGATCAAGCTGAGTATCCATTTAGTTATGAAGCTGCTTTGAACAGTCCATGACATGTCGATTATACCTTTCAAATTGAATTATCTTATAAATCCAAATGTATCGTACCTTTATGTTTGATTTTAGTTATTCAACATTTGTTTACCTTTGAGGTTCTCTGGCCGCAGGAGATATGTAATTTTGGACGGTTTGTTGAATCAGCTGCCTCAACCGGAAATGTATCAGGTAATTTACCTGTATGAAAACCCCATGGGATAGACAAGTTTTGTACTTCGTGCGATCTACTTTCTTTGGTTGTTCTTTTTCTTTAAATCATCTGAGAATTTACTCATATCATCTTTCTTGCATGCAAAACATTGTGATCATCCATGTTTTCATCGTTCAGTGATGTATGTTGTCGACCTTACGATGGCTGCCTATTACCAAAGTGTCACGATCCAGATTGTGGAGGTGCTCAAATAAAACTTAATTGTCAACTTTTTTTCGGAAAAATATTTATTTGCAGCCATTGCTTGATTGAACTTATCACCCTACAGGTTACCGATCTCCACTGCGTCCAAGTCCAATTGATGCCATTCCTAATCCTTGGGTCTCCACGGATTCAGAACAGATATCAAATGACATAG

mRNA sequence

ATGCTTCAAGTGCGGCGTTTCTCATCCTTAATCTTCCGTGAGAGGAAAAAAGAAAGAAAAATTACATTGAAGCCTGAGAGTTCAGCAAACTTTGAGACTCCAGTTTCTGATTCAGTTAAGGAAATACAGGACAATGCAACGAACGGCCAAGATTTCATGGAAAAATGTGAGGAAGACATGTTCATGGCATCCTTGGTCTGCAACGTCGATGCTGAGTGTGAGAAACTTCTGGTTTGCGTAACGGAATTACACTGCGTAAAGACATTGCAATTTGAAATGGAGGAGATATGTAATTTTGGACGGTTTGTTGAATCAGCTGCCTCAACCGGAAATGTATCAGTGATGTATGTTGTCGACCTTACGATGGCTGCCTATTACCAAAGTGTCACGATCCAGATTGTGGAGGTTACCGATCTCCACTGCGTCCAAGTCCAATTGATGCCATTCCTAATCCTTGGGTCTCCACGGATTCAGAACAGATATCAAATGACATAG

Coding sequence (CDS)

ATGCTTCAAGTGCGGCGTTTCTCATCCTTAATCTTCCGTGAGAGGAAAAAAGAAAGAAAAATTACATTGAAGCCTGAGAGTTCAGCAAACTTTGAGACTCCAGTTTCTGATTCAGTTAAGGAAATACAGGACAATGCAACGAACGGCCAAGATTTCATGGAAAAATGTGAGGAAGACATGTTCATGGCATCCTTGGTCTGCAACGTCGATGCTGAGTGTGAGAAACTTCTGGTTTGCGTAACGGAATTACACTGCGTAAAGACATTGCAATTTGAAATGGAGGAGATATGTAATTTTGGACGGTTTGTTGAATCAGCTGCCTCAACCGGAAATGTATCAGTGATGTATGTTGTCGACCTTACGATGGCTGCCTATTACCAAAGTGTCACGATCCAGATTGTGGAGGTTACCGATCTCCACTGCGTCCAAGTCCAATTGATGCCATTCCTAATCCTTGGGTCTCCACGGATTCAGAACAGATATCAAATGACATAG

Protein sequence

MLQVRRFSSLIFRERKKERKITLKPESSANFETPVSDSVKEIQDNATNGQDFMEKCEEDMFMASLVCNVDAECEKLLVCVTELHCVKTLQFEMEEICNFGRFVESAASTGNVSVMYVVDLTMAAYYQSVTIQIVEVTDLHCVQVQLMPFLILGSPRIQNRYQMT
Homology
BLAST of Csor.00g305220 vs. NCBI nr
Match: KAG6574204.1 (hypothetical protein SDJN03_28091, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 315 bits (807), Expect = 4.19e-108
Identity = 164/164 (100.00%), Postives = 164/164 (100.00%), Query Frame = 0

Query: 1   MLQVRRFSSLIFRERKKERKITLKPESSANFETPVSDSVKEIQDNATNGQDFMEKCEEDM 60
           MLQVRRFSSLIFRERKKERKITLKPESSANFETPVSDSVKEIQDNATNGQDFMEKCEEDM
Sbjct: 1   MLQVRRFSSLIFRERKKERKITLKPESSANFETPVSDSVKEIQDNATNGQDFMEKCEEDM 60

Query: 61  FMASLVCNVDAECEKLLVCVTELHCVKTLQFEMEEICNFGRFVESAASTGNVSVMYVVDL 120
           FMASLVCNVDAECEKLLVCVTELHCVKTLQFEMEEICNFGRFVESAASTGNVSVMYVVDL
Sbjct: 61  FMASLVCNVDAECEKLLVCVTELHCVKTLQFEMEEICNFGRFVESAASTGNVSVMYVVDL 120

Query: 121 TMAAYYQSVTIQIVEVTDLHCVQVQLMPFLILGSPRIQNRYQMT 164
           TMAAYYQSVTIQIVEVTDLHCVQVQLMPFLILGSPRIQNRYQMT
Sbjct: 121 TMAAYYQSVTIQIVEVTDLHCVQVQLMPFLILGSPRIQNRYQMT 164

BLAST of Csor.00g305220 vs. NCBI nr
Match: XP_016899008.1 (PREDICTED: DNA-directed primase/polymerase protein isoform X2 [Cucumis melo])

HSP 1 Score: 105 bits (261), Expect = 9.04e-23
Identity = 58/109 (53.21%), Postives = 66/109 (60.55%), Query Frame = 0

Query: 52  FMEKCEEDMFMASLVCNVDAECEKLLVCVTELHCVKTLQFEMEEICNFGR---------- 111
           + + CEEDMFMASL+CNVDAECEKLLVC  +L C+KTLQFE EE CNFGR          
Sbjct: 342 YTKMCEEDMFMASLICNVDAECEKLLVCKMDLDCIKTLQFETEEKCNFGRNVSFTQEAVL 401

Query: 112 -----------------------FVESAASTGNVSVMYVVDLTMAAYYQ 127
                                  FV+S ASTG+V+VMYVVDL  AAYYQ
Sbjct: 402 TGSMRDISATSCMGKSPFPDVDKFVQSVASTGSVAVMYVVDLRSAAYYQ 450

BLAST of Csor.00g305220 vs. NCBI nr
Match: KAG7013266.1 (hypothetical protein SDJN02_26024, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 95.9 bits (237), Expect = 1.50e-21
Identity = 62/120 (51.67%), Postives = 70/120 (58.33%), Query Frame = 0

Query: 37  DSVKEIQDNATNGQDFMEKC---------------------------EEDMFMASLV--C 96
           +S KEIQDNATNGQDFMEK                            E+  F  S++  C
Sbjct: 4   ESRKEIQDNATNGQDFMEKSYNSPRGVPPRRPSSLWRLLLEIRVELSEQSRFKLSVMKTC 63

Query: 97  NVDAECEKLLVCVTELHCVKTLQFEMEEICNFGRFVESAASTGNVSVMYVVDLTMAAYYQ 127
           +        ++ V    CVKTLQF+MEEICNFGRFVESAASTGNVSVMYVVDLT A YYQ
Sbjct: 64  SWHPWSATSMLSVRNF-CVKTLQFDMEEICNFGRFVESAASTGNVSVMYVVDLTTAVYYQ 122

BLAST of Csor.00g305220 vs. NCBI nr
Match: TYK23011.1 (DNA-directed primase/polymerase protein isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 95.5 bits (236), Expect = 4.53e-21
Identity = 54/101 (53.47%), Postives = 60/101 (59.41%), Query Frame = 0

Query: 60  MFMASLVCNVDAECEKLLVCVTELHCVKTLQFEMEEICNFGR------------------ 119
           MFMASL+CNVDAECEKLLVC  +L C+KTLQFE EE CNFGR                  
Sbjct: 1   MFMASLICNVDAECEKLLVCKMDLDCIKTLQFETEEKCNFGRNVSFTQEAVMTGSMRDIS 60

Query: 120 ---------------FVESAASTGNVSVMYVVDLTMAAYYQ 127
                          FV+S ASTG+V+VMYVVDL  AAYYQ
Sbjct: 61  ATSCMGKSPFPDVDKFVQSVASTGSVAVMYVVDLRSAAYYQ 101

BLAST of Csor.00g305220 vs. NCBI nr
Match: KAG6576693.1 (DNA-directed primase/polymerase protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 91.7 bits (226), Expect = 5.05e-18
Identity = 61/141 (43.26%), Postives = 66/141 (46.81%), Query Frame = 0

Query: 56  CEEDMFMASLVCNVDAECEKLLVCVTELHCVKTLQFEMEEICNFGR-------------- 115
           CEEDMFMASL+CNVDA+C+KLLVC T+L CVKTLQFEMEE CNFGR              
Sbjct: 347 CEEDMFMASLICNVDADCDKLLVCKTDLDCVKTLQFEMEEKCNFGRQFSFAKEAPLTGYT 406

Query: 116 -------------------FVESAASTGNVS----------------------------- 127
                              FVES ASTG+VS                             
Sbjct: 407 NDVPASSCMGTSPFPAVDKFVESVASTGSVSGKIRCWYWFSEYGLIVYSMSRNRYCERIG 466

BLAST of Csor.00g305220 vs. ExPASy TrEMBL
Match: A0A1S4DSS4 (DNA-directed primase/polymerase protein isoform X2 OS=Cucumis melo OX=3656 GN=LOC103484132 PE=4 SV=1)

HSP 1 Score: 105 bits (261), Expect = 4.38e-23
Identity = 58/109 (53.21%), Postives = 66/109 (60.55%), Query Frame = 0

Query: 52  FMEKCEEDMFMASLVCNVDAECEKLLVCVTELHCVKTLQFEMEEICNFGR---------- 111
           + + CEEDMFMASL+CNVDAECEKLLVC  +L C+KTLQFE EE CNFGR          
Sbjct: 342 YTKMCEEDMFMASLICNVDAECEKLLVCKMDLDCIKTLQFETEEKCNFGRNVSFTQEAVL 401

Query: 112 -----------------------FVESAASTGNVSVMYVVDLTMAAYYQ 127
                                  FV+S ASTG+V+VMYVVDL  AAYYQ
Sbjct: 402 TGSMRDISATSCMGKSPFPDVDKFVQSVASTGSVAVMYVVDLRSAAYYQ 450

BLAST of Csor.00g305220 vs. ExPASy TrEMBL
Match: A0A5D3DH78 (DNA-directed primase/polymerase protein isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold386G001190 PE=4 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 2.19e-21
Identity = 54/101 (53.47%), Postives = 60/101 (59.41%), Query Frame = 0

Query: 60  MFMASLVCNVDAECEKLLVCVTELHCVKTLQFEMEEICNFGR------------------ 119
           MFMASL+CNVDAECEKLLVC  +L C+KTLQFE EE CNFGR                  
Sbjct: 1   MFMASLICNVDAECEKLLVCKMDLDCIKTLQFETEEKCNFGRNVSFTQEAVMTGSMRDIS 60

Query: 120 ---------------FVESAASTGNVSVMYVVDLTMAAYYQ 127
                          FV+S ASTG+V+VMYVVDL  AAYYQ
Sbjct: 61  ATSCMGKSPFPDVDKFVQSVASTGSVAVMYVVDLRSAAYYQ 101

BLAST of Csor.00g305220 vs. ExPASy TrEMBL
Match: A0A6J1E4L8 (DNA-directed primase/polymerase protein OS=Cucurbita moschata OX=3662 GN=LOC111430698 PE=4 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 2.48e-18
Identity = 61/141 (43.26%), Postives = 66/141 (46.81%), Query Frame = 0

Query: 56  CEEDMFMASLVCNVDAECEKLLVCVTELHCVKTLQFEMEEICNFGR-------------- 115
           CEEDMFMASL+CNVDA+C+KLLVC T+L CVKTLQFEMEE CNFGR              
Sbjct: 347 CEEDMFMASLICNVDADCDKLLVCKTDLDCVKTLQFEMEEKCNFGRQFSFAKEAPLTGYM 406

Query: 116 -------------------FVESAASTGNVS----------------------------- 127
                              FVES ASTG+VS                             
Sbjct: 407 NDVPASSCMGTSPFPAVDKFVESVASTGSVSGKIRCWYWFSEYGLIVYSMSRNRYCERIG 466

BLAST of Csor.00g305220 vs. ExPASy TrEMBL
Match: A0A6J1JB70 (DNA-directed primase/polymerase protein-like OS=Cucurbita maxima OX=3661 GN=LOC111482880 PE=4 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 2.16e-17
Identity = 59/141 (41.84%), Postives = 65/141 (46.10%), Query Frame = 0

Query: 56  CEEDMFMASLVCNVDAECEKLLVCVTELHCVKTLQFEMEEICNFGR-------------- 115
           CEEDMFMASL+CNVDA+CEKLLVC T+  CV+TLQFEMEE CNFGR              
Sbjct: 347 CEEDMFMASLICNVDADCEKLLVCKTDFDCVRTLQFEMEEKCNFGRQFSFAKEAPLTGYM 406

Query: 116 -------------------FVESAASTGNVS----------------------------- 127
                              FV+S ASTG+VS                             
Sbjct: 407 NDVSASSCMGTSPFPALDKFVQSVASTGSVSGKIRCWYWFSEYGLIVYSMSRNRYCERIG 466

BLAST of Csor.00g305220 vs. ExPASy TrEMBL
Match: A0A0A0L6Q1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G184000 PE=4 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 2.95e-17
Identity = 58/141 (41.13%), Postives = 65/141 (46.10%), Query Frame = 0

Query: 56  CEEDMFMASLVCNVDAECEKLLVCVTELHCVKTLQFEMEEICNFG--------------- 115
           CEEDMFMASL+CNVDA+CEKLLVC  +L C+KTLQFEMEE CNFG               
Sbjct: 346 CEEDMFMASLICNVDADCEKLLVCKMDLDCIKTLQFEMEEKCNFGHNFSFTKEAVLTGSM 405

Query: 116 ------------------RFVESAASTGNVS----------------------------- 127
                             +FV+S ASTGNV+                             
Sbjct: 406 RDFSATSCMGKSPFPDVDKFVQSVASTGNVAGKIRCWYWFSEFGLIVYSMSSNRYCERIG 465

BLAST of Csor.00g305220 vs. TAIR 10
Match: AT5G52800.1 (DNA primases )

HSP 1 Score: 52.8 bits (125), Expect = 3.1e-07
Identity = 23/38 (60.53%), Postives = 30/38 (78.95%), Query Frame = 0

Query: 57  EEDMFMASLVCNVDAECEKLLVCVTELHCVKTLQFEME 95
           E D+FM SL+CNV+++CEKLLVC  E  C+KTL F+ E
Sbjct: 358 ERDVFMTSLICNVESDCEKLLVCKMESDCMKTLCFDTE 395

BLAST of Csor.00g305220 vs. TAIR 10
Match: AT5G52800.2 (DNA primases )

HSP 1 Score: 52.8 bits (125), Expect = 3.1e-07
Identity = 23/38 (60.53%), Postives = 30/38 (78.95%), Query Frame = 0

Query: 57  EEDMFMASLVCNVDAECEKLLVCVTELHCVKTLQFEME 95
           E D+FM SL+CNV+++CEKLLVC  E  C+KTL F+ E
Sbjct: 358 ERDVFMTSLICNVESDCEKLLVCKMESDCMKTLCFDTE 395

BLAST of Csor.00g305220 vs. TAIR 10
Match: AT5G52800.3 (DNA primases )

HSP 1 Score: 52.8 bits (125), Expect = 3.1e-07
Identity = 23/38 (60.53%), Postives = 30/38 (78.95%), Query Frame = 0

Query: 57  EEDMFMASLVCNVDAECEKLLVCVTELHCVKTLQFEME 95
           E D+FM SL+CNV+++CEKLLVC  E  C+KTL F+ E
Sbjct: 358 ERDVFMTSLICNVESDCEKLLVCKMESDCMKTLCFDTE 395

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
KAG6574204.14.19e-108100.00hypothetical protein SDJN03_28091, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_016899008.19.04e-2353.21PREDICTED: DNA-directed primase/polymerase protein isoform X2 [Cucumis melo][more]
KAG7013266.11.50e-2151.67hypothetical protein SDJN02_26024, partial [Cucurbita argyrosperma subsp. argyro... [more]
TYK23011.14.53e-2153.47DNA-directed primase/polymerase protein isoform X2 [Cucumis melo var. makuwa][more]
KAG6576693.15.05e-1843.26DNA-directed primase/polymerase protein, partial [Cucurbita argyrosperma subsp. ... [more]
Match NameE-valueIdentityDescription
A0A1S4DSS44.38e-2353.21DNA-directed primase/polymerase protein isoform X2 OS=Cucumis melo OX=3656 GN=LO... [more]
A0A5D3DH782.19e-2153.47DNA-directed primase/polymerase protein isoform X2 OS=Cucumis melo var. makuwa O... [more]
A0A6J1E4L82.48e-1843.26DNA-directed primase/polymerase protein OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1JB702.16e-1741.84DNA-directed primase/polymerase protein-like OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A0A0L6Q12.95e-1741.13Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G184000 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G52800.13.1e-0760.53DNA primases [more]
AT5G52800.23.1e-0760.53DNA primases [more]
AT5G52800.33.1e-0760.53DNA primases [more]
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g305220.m01Csor.00g305220.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006261 DNA-dependent DNA replication
biological_process GO:0019985 translesion synthesis
molecular_function GO:0140640 catalytic activity, acting on a nucleic acid
molecular_function GO:0016779 nucleotidyltransferase activity