Csor.00g297240 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g297240
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	primase homolog protein-like isoform X1
Location	Csor_Chr18: 3514867 .. 3520266 (-)
RNA-Seq Expression	Csor.00g297240
Synteny	Csor.00g297240

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAGCTTCGATTCTGCTCCCCATTGTTCTCCAAATCCGTCGGATTCAAATGCCAATGAAAACCCCTTTCAATTTCAACTTCAATCATCGCCCTGCTATTTTCTGTCATCCGCTTAACAATTTCTCCTTCTCTTCCCCTTCTCTTCCACTCTACCCAACCCGATTATTATGCGCTCTTTCTCCTACTCCGCCGTCGCCGTCGCCATCGCCACCACCAGGTACTTAATCGTTTCGGTCCTGTTATAATACTCTTTTTCTTTGTTTACGGTTTTAGGGTTAATGTTATGGGCGAATCTAAAGTGAAGGTTCTAATGGAGAAAATGGATCTTATCGGAATAGTCTGTGATGAACAATGTACTCCTGGAAAATACTATTGCCTGCTCTGTCCCAAGGTTATCTGCTCCTCCCGTCTATGCCTTTCGTGGTTTCTATACATGAAAATCAAATCCATATTCTTGTTTTGATGGTTGAACAGTGCAAGGGTGGGCGCTTAATGGAGAGGAGCTTATCTCTACATGTAATTCCAACAGGGCAAGTGATTATTACTGTTTTTCCCTTGATTTTGTGAGGATTGATTGGCAGTAAATTGTTCATGAATTACATTTTGTTATTGGGAACAGAGATTTTGCAATGTGGAGGTGTTTCCAGTCTGAGTGTGGTTGGGCAGGCCGGGTAACGTCATATTAATATGTTCAATCTCTTATGTAGCAATTAACACATGAGAATTTCTTAGCCAAATGATTGTTTGGTTTAGATATTTGCAGATGGAAGATTGGCGTTTAATGAGTTCAGCAGAACTGCAAAGTTGTTTGGAGGAACTGCAAAGAAGAGTCTTATTTTGGAGCCTCTTGGTGATGAGGTACTTCGAATTTCTTTGCATTTCTATGTATAAGACTAATACGTTAGTTGTGAGACCCCACATTGGTTGGAGAGAAAATCTCTTCCTAGTAGACGTGTTTTAAAACTGTAAGGCTGACGGCGATACGTAAGGGGCTAATGTCTACTAGCGGTGGGCTTGGGCTGTTACAAATGGTATTAGAGCCAGACACCAGGTGGTGTGCCAGTGAGGACACTGGGCCCCCAAGGGGGGTGGATTGTGAGATCTCACATCTGTTGGAGAGGAGAACGAAGCATTGCTTATAAGGTTGTGGAAACCTCTCCCTACCAGATGCGATTTAAAAACCTTGAGGGGAAAGCCTAGGAGGGATAGCCCAAAAAGGACAATATTTGCTAGTGGTAGAATTGGGTGGTTACAAATGGTATCAGAGCTAGACACCAGGCCAGCTAGCGAGGACGATGGGCCTCAAGGGGGGTAGATTGTGAGATCCCACATCGATTGGAGGAGGAGAACGAAGTATTCCTTATAAGGGTATGGAAACCTTTCCCTAGTAGACATGTTTTAAAATTGTAAGGCTGATGGTGATATATAACGGGCTAAAATGAAGAATATCTGCTAGGAGTGGGCTTGGACTGATACATTAGTATTGTTTGAATGGGTTAACTTGTGCAATCTTTTGACAGAAACACGGTTAATTTTGACTGCTAGGTAGTCCTACATGTTACGATGAATAAGACTAGGAAGACTTTTGATCTTAATGCAAACTGTTGAATTTATCTTTGAATTTGAGGTTCAAGTGTTTTGATGTTTCTTTGTCAGCTAATCACTTACTTCAGCAAGAGAATGATATCCCAGGAAACTCTCAAAAGGAATGTTGTTATGCAAACAGCCGATCGTCAGGTACCTGAAAGAATGCTTTTGTTTGTATGCCTTTCATTGAATTTTTAGACTCGTACCCTTGTGGATGCAATACTGCCTAGAAGTTATGTAACAACCCAAGCCCAAGCCCACCGCTAGCCGATATTATCCTCTTTGGATTTTCCCTTTCGAGCTTCCCTTCAAGGTTTTTAAAACGTGTCTGCAAGGGAGAGCTTTCCACACCCATATAAAGAATGTTTCGTTCTCCTCCACAATCGATGTGGGATCTCACAATCCACCCCCCTTCGGGGCCCAGCGTCCTCGCTGCACTCGTTCCCTTATCTAATCGATGTGGGACCCTCCAATCCACCCCCTTTCGGGGCCCAACGTCCTTGCTGGTACACCACCTCGTGCACACCTCCTTCGGGGCTTAGCCTCCTCACTGACACATCGCCCAGTGTTTAGCTCTAATACCATTTGTAACAGCTCAAGCCCACCGCTAGCAAATATTGTCCTCTTTAGGTTTTTCCTTTCGGGCTTCCCTTCAAGGTTTTTTAAAACGTAGGGAGAGGTCTCCACACTCTTGTAAAGAATGCTTCGTTCTCCTCTCCAACCGATGCGGGATCTCACAAACGCGTCTCTCTCATTTCCTTGTAGCTTCAACTTGTGACTAGTTTAAAGGTCACTTGCAGGCTGCAATTGCATTCACTTATCGACAAAACGGACAGCTTGTTGGCTGTAAGTACCGAACCATAAACAAGAAATTTTGGCAGGTACATCAATCAATCCTTCTTCAATCAGAGTTATTTTAGAAACTGTCCAGCATCTTCCTTCCTGTTCTTCGGCTTGTAATTCTAAGCGTTTCTAAGCTTTCTAATCTGTATGTCTGATATCAATATTCAGGCGAAGGGCACAGAAAAATTGTTTTATGGGATTGATGATATTAATGATGCAGATGAACTCATCATTGTAAGAATTCACAATACCTTGTTTTGTAAGTTTGTATTTTATATTTTGATCTATTTTTCCATCAATTTTTGATAATGTTGTGTATGTCGATTAAGGTCGAAGGTGAAATGGATAAACTTTCAGTAGAAGAAGCTGGCTTCCTGAATTGTATCAGTGTTCCTGGTGGTGCCCCAACTAAGGTTTCAACTAATGCAGTAGCATCAATTCAAAAGGTTCTGTGTTATAGAAATTCATCTATGCCTATCTTGTTTGCTTTTACATCATTCCTAGCTATCTGAGTTATGCTACTAAACTATTTCTCTCTTCCCTTGATCCCACATCGGCTGGAGAGGGGAACAAATCATTTTTTATAAGGGTGTGGCAACATTTCCCTAGCAGAAGCGTTTTAAAAGCTTGGGTTGTTACAAATGGTATTAGAGCCAGACATCGGGCGATGTGTCAGCGAGGAGGCTGAGCCCTGAAGAGGGGTGGAGCCAGCAAGGACGTTGGGCCCCGAAAGGGGTGGATTGGGGGGTCTCATATCGATTGGAGAAGGGAACGAGTGCTAGCGACGATGCTGGTCCCGAAGGGGGATGGATCGTGAGATCCCACATTGGTTGGGGAGGAGAATGAAACATTTTTTATAAGGGTGTGGAAACTTCTCCCTAGCATACGCGTTTTAAAATCATGAGGGGAAGCCCAAAAAGAAAAACCCAAAGAGAACAATATCTGCTAGTGGTGGACTTGATCTGTTACGTTACAGAACTTGAAATGTGGTTTAAAATGGAGTAAAACACACCTTTGATACTCAAAGATTTAAATTTATTATCTATTTGGTCATTTAGTTCTTGAAATTTGAATACGTTTCTATGTGTTTTGAGTTTCATTTTTAATAGATCCCGAAAGTTTGGAATTGGTTTCTAATTGGTCCTTCAGCGTAAAAAAGTTAAAAACTTCAAATTTTAGAGACCAATTAGAAACTAGATTCAAAATTCGAGGACAAAGCATGACATTTTTAGGATTTCAAAGACCAGGTAGAAACTAAATTTCAATCTAAAAAACTAGACTGTGTATTGGTTCATCTTTAGAAAGTTACGACAAGAAGGGTCACTAAAGCATCGTATAAGAAACGCTCTACACCCGTTCCAACTAGAAACCAAAGATAAGAAGAACTGATTCTAAGTTAAAACTTTTGTGATAATATTAGGTTTAGCTATGATGATCCCATATGGTTTACAAATTCATTGTTCTTAATTTATATGGTTTCACATGTCAGGATACTGCATATCAGTATTTATGGAGCTGCAAAGACTTGTTAGATAAGGTAAATAATATGGTCTGGTCGGGTAGGCTTTGTTTGATAATCATTTGGTTTATCCTTGATTTTGAAAACACAGGCAGTGGACAACAAGAAAAGATCAAATGGTTATCAAATGGAGCGTTCATTAACGATTTTGCTGCTTTTTCCTTTTATGTTAGAAATAAGCACGTTAACAGCTTCGTCGTTTTGTAATTTACATTTTGAAAACGTGTTTTAAAGTTTGAAATACTAACAAAATTATTAACCTGAAAGGTTCCCCGGATTATCTTGGCTACTGATAGTGATGAACCAGGGCAAGCTTTGGCAGAAGAGCTAGCACTTCGGCTTGGAAAACATAGGTCTCCACTCAGTTGTGAACTTTGTGTTAATCTTAACGCTTCATTTGTTTTTCATAAAGGCAAAATAACGTGGCTTTGTTGTTTTGGTTAACAGATGTTGGCGGGTAGATTGGCCTTATAAAGATGACTTCAACCGTTTTAAAGATGCCAATGAGGTTACATTCCTCCCTATATTACTCTTTTTAAGTTATTGTTTTTTGGGATTGGTGTTGTGCCATAGTGCCAAACCTTCCAACCTTCTATGCTCTGATATCACTTGTTGTGTCGTAATGCCACAACCTTCAATATTCTGATACCACTTGTTGTACCCAAATACCACGACCAAAATGTTCTAATACCCAATTGTTGTTGCGGAAGCGGAAGCAATATTGATCGCACTCACACACCCACAAACAAAAATTAAACAATGAGATGCAAAGATTTACGTGGTTCGGAGAGAAGAAATTCCCCTACATCCACGAGTGTTTACAAATGTATCTCTCACACTCTCACACTACAAACTCTCTCAACAAAATTCCTCCCAATTGCCTCTAGACAAATATAGAACGACAATGACTCCACACCTCAAAACAATGCTAGACAAGTCTTTGTATAGGCATCATGAACTAAATAAAAGAGAATATGAACCAAAAGGGTTATAAACTAAATGAGAGTTAATAACCCGGTGAAGTCAAGCATCCTAAAGCTAATTATAGATCATTATTCTATGATGTACGTTTTATATTCTTACTACTGTTGTTCAAAATCTGGAAAACGGCAGGGATGGCAAAAAGAGGGGGGCTATTGTGTCGTTAATTCAAACCTTCTTTCTTGGGATGATGATATTTATTTTGATCCTTTTGGCATTTGAATGTGTTATTACAAACAGATTCTGGTGCAGTTGGGGGCAGATGCTTTAAAGAAAGCAATTCAAGATGCCAAGTCATATCAACTTTGTGATACCGATGAAATACAAGTAGGTGGGTATCCAAATATTTCCAGAAAATTCCCATTTTTAAAATGCTTTTGATTTGGGTTTTGGTAATTATTTTGATGACCTTCCTCTCTTTTAACTTTGCAGAAGAGGATCTCGAGAGCTTCCATGAGCCTTCGTAA

mRNA sequence

ATGGCAGCTTCGATTCTGCTCCCCATTGTTCTCCAAATCCGTCGGATTCAAATGCCAATGAAAACCCCTTTCAATTTCAACTTCAATCATCGCCCTGCTATTTTCTGTCATCCGCTTAACAATTTCTCCTTCTCTTCCCCTTCTCTTCCACTCTACCCAACCCGATTATTATGCGCTCTTTCTCCTACTCCGCCGTCGCCGTCGCCATCGCCACCACCAGTGAAGGTTCTAATGGAGAAAATGGATCTTATCGGAATAGTCTGTGATGAACAATGTACTCCTGGAAAATACTATTGCCTGCTCTGTCCCAAGTGCAAGGGTGGGCGCTTAATGGAGAGGAGCTTATCTCTACATGTAATTCCAACAGGAGATTTTGCAATGTGGAGGTGTTTCCAGTCTGAGTGTGGTTGGGCAGGCCGGATATTTGCAGATGGAAGATTGGCGTTTAATGAGTTCAGCAGAACTGCAAAGTTGTTTGGAGGAACTGCAAAGAAGAGTCTTATTTTGGAGCCTCTTGGTGATGAGCTAATCACTTACTTCAGCAAGAGAATGATATCCCAGGAAACTCTCAAAAGGAATGTTGTTATGCAAACAGCCGATCGTCAGGCTGCAATTGCATTCACTTATCGACAAAACGGACAGCTTGTTGGCTGTAAGTACCGAACCATAAACAAGAAATTTTGGCAGGCGAAGGGCACAGAAAAATTGTTTTATGGGATTGATGATATTAATGATGCAGATGAACTCATCATTGTCGAAGGTGAAATGGATAAACTTTCAGTAGAAGAAGCTGGCTTCCTGAATTGTATCAGTGTTCCTGGTGGTGCCCCAACTAAGGTTTCAACTAATGCAGTAGCATCAATTCAAAAGGATACTGCATATCAGTATTTATGGAGCTGCAAAGACTTGTTAGATAAGGTTCCCCGGATTATCTTGGCTACTGATAGTGATGAACCAGGGCAAGCTTTGGCAGAAGAGCTAGCACTTCGGCTTGGAAAACATAGATGTTGGCGGGTAGATTGGCCTTATAAAGATGACTTCAACCGTTTTAAAGATGCCAATGAGATTCTGGTGCAGTTGGGGGCAGATGCTTTAAAGAAAGCAATTCAAGATGCCAAGTCATATCAACTTTGTGATACCGATGAAATACAAGTAGAAGAGGATCTCGAGAGCTTCCATGAGCCTTCGTAA

Coding sequence (CDS)

ATGGCAGCTTCGATTCTGCTCCCCATTGTTCTCCAAATCCGTCGGATTCAAATGCCAATGAAAACCCCTTTCAATTTCAACTTCAATCATCGCCCTGCTATTTTCTGTCATCCGCTTAACAATTTCTCCTTCTCTTCCCCTTCTCTTCCACTCTACCCAACCCGATTATTATGCGCTCTTTCTCCTACTCCGCCGTCGCCGTCGCCATCGCCACCACCAGTGAAGGTTCTAATGGAGAAAATGGATCTTATCGGAATAGTCTGTGATGAACAATGTACTCCTGGAAAATACTATTGCCTGCTCTGTCCCAAGTGCAAGGGTGGGCGCTTAATGGAGAGGAGCTTATCTCTACATGTAATTCCAACAGGAGATTTTGCAATGTGGAGGTGTTTCCAGTCTGAGTGTGGTTGGGCAGGCCGGATATTTGCAGATGGAAGATTGGCGTTTAATGAGTTCAGCAGAACTGCAAAGTTGTTTGGAGGAACTGCAAAGAAGAGTCTTATTTTGGAGCCTCTTGGTGATGAGCTAATCACTTACTTCAGCAAGAGAATGATATCCCAGGAAACTCTCAAAAGGAATGTTGTTATGCAAACAGCCGATCGTCAGGCTGCAATTGCATTCACTTATCGACAAAACGGACAGCTTGTTGGCTGTAAGTACCGAACCATAAACAAGAAATTTTGGCAGGCGAAGGGCACAGAAAAATTGTTTTATGGGATTGATGATATTAATGATGCAGATGAACTCATCATTGTCGAAGGTGAAATGGATAAACTTTCAGTAGAAGAAGCTGGCTTCCTGAATTGTATCAGTGTTCCTGGTGGTGCCCCAACTAAGGTTTCAACTAATGCAGTAGCATCAATTCAAAAGGATACTGCATATCAGTATTTATGGAGCTGCAAAGACTTGTTAGATAAGGTTCCCCGGATTATCTTGGCTACTGATAGTGATGAACCAGGGCAAGCTTTGGCAGAAGAGCTAGCACTTCGGCTTGGAAAACATAGATGTTGGCGGGTAGATTGGCCTTATAAAGATGACTTCAACCGTTTTAAAGATGCCAATGAGATTCTGGTGCAGTTGGGGGCAGATGCTTTAAAGAAAGCAATTCAAGATGCCAAGTCATATCAACTTTGTGATACCGATGAAATACAAGTAGAAGAGGATCTCGAGAGCTTCCATGAGCCTTCGTAA

Protein sequence

MAASILLPIVLQIRRIQMPMKTPFNFNFNHRPAIFCHPLNNFSFSSPSLPLYPTRLLCALSPTPPSPSPSPPPVKVLMEKMDLIGIVCDEQCTPGKYYCLLCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQSECGWAGRIFADGRLAFNEFSRTAKLFGGTAKKSLILEPLGDELITYFSKRMISQETLKRNVVMQTADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGIDDINDADELIIVEGEMDKLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLWSCKDLLDKVPRIILATDSDEPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILVQLGADALKKAIQDAKSYQLCDTDEIQVEEDLESFHEPS

Homology

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BLAST of Csor.00g297240 vs. ExPASy Swiss-Prot
Match: B5X582 (Twinkle homolog protein, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g30680 PE=1 SV=1)

HSP 1 Score: 306.2 bits (783), Expect = 5.3e-82
Identity = 155/313 (49.52%), Postives = 201/313 (64.22%), Query Frame = 0


Query: 90  EQCTPGKYYCLLCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQSECGWAGRIFADGRLAF 149
           E C PG++  L+CP C+GG   E+SLSL + P G  A W CF+ +CG  G + ADG LA 
Sbjct: 124 ENCPPGQHSGLICPTCEGGNSGEKSLSLFIAPDGSSATWNCFRGKCGLKGGVRADGGLA- 183

Query: 150 NEFSRTAKLFGGTAKKSLILEPLGDELITYFSKRMISQETLKRNVVMQ-TADRQAAIAFT 209
                  K+      + + LEPL DE+  YF+ R IS++TL+RN VMQ     +  IAFT
Sbjct: 184 -SADPIEKVERKITVEGIELEPLCDEIQDYFAARAISRKTLERNRVMQKRIGDEIVIAFT 243

Query: 210 YRQNGQLVGCKYRTINKKFWQAKGTEKLFYGIDDINDADELIIVEGEMDKLSVEEAGFLN 269
           Y Q G+LV CKYR++ K F+Q + T ++ YG+DDI    E+IIVEGE+DKL++EEAGFLN
Sbjct: 244 YWQRGELVSCKYRSLTKMFFQERKTRRILYGLDDIEKTSEVIIVEGEIDKLAMEEAGFLN 303

Query: 270 CISVPGGAPTKVSTNAVASIQKDTAYQYLWSCKDLLDKVPRIILATDSDEPGQALAEELA 329
           C+SVP GAP KVS+  + S  KDT Y++LW+C D L K  RI++ATD D PGQA+AEE+A
Sbjct: 304 CVSVPDGAPAKVSSKEIPSEDKDTKYKFLWNCNDYLKKASRIVIATDGDGPGQAMAEEIA 363

Query: 330 LRLGKHRCWRVDWPYKDDFNRFKDANEILVQLGADALKKAIQDAKSYQLCDT-------D 389
            RLGK RCWRV WP K +   FKDANE+L+  G   LK+AI DA+ Y +          D
Sbjct: 364 RRLGKERCWRVKWPKKSEDEHFKDANEVLMSKGPHLLKEAILDAEPYPILGLFSFKDFFD 423

Query: 390 EIQVEEDLESFHE 395
           EI    D    HE
Sbjct: 424 EIDAYYDRTHGHE 434

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BLAST of Csor.00g297240 vs. ExPASy Swiss-Prot
Match: F4I6E6 (Primase homolog protein OS=Arabidopsis thaliana OX=3702 GN=At1g30660 PE=3 SV=1)

HSP 1 Score: 299.7 bits (766), Expect = 5.0e-80
Identity = 152/302 (50.33%), Postives = 199/302 (65.89%), Query Frame = 0


Query: 77  LMEKMDLIGIVCDEQCTPGKYYCLLCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQSECG 136
           LM K+   GI   + C PG   CL+CPKC+ G   E+SL+L++ P G  A W C + +CG
Sbjct: 38  LMRKLSEQGIDA-QNCPPGVRSCLICPKCEVGDSGEKSLTLYIYPDGSSAKWTC-RRKCG 97

Query: 137 WAGRIFADGRLAFNEFSRTAKLFGGTAKKSLILEPLGDELITYFSKRMISQETLKRNVVM 196
             G +  DG+L   +     K+      +S+ LEPL DE+  +F+ R IS +TL+RN VM
Sbjct: 98  LKGVLQVDGKLVSKD--PIGKVERKITVESIKLEPLCDEIQDFFAARAISGKTLERNRVM 157

Query: 197 Q-TADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGIDDINDADELIIVEGE 256
           Q   D +  IAFTY Q G+LV CKYR++ KKF Q + T K+ YG+DDI +  E+IIVEGE
Sbjct: 158 QKRIDDEIVIAFTYWQRGELVSCKYRSLTKKFVQERNTRKILYGLDDIEETSEIIIVEGE 217

Query: 257 MDKLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLWSCKDLLDKVPRIILATD 316
            DKL++EEAGF NC+SVP GAP  VS+  + S  KDTA++Y+W+C D L K  RI++ATD
Sbjct: 218 PDKLAMEEAGFFNCVSVPDGAPETVSSKEIPSESKDTAFKYIWNCNDYLKKASRIVIATD 277

Query: 317 SDEPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILVQLGADALKKAIQDAKSY 376
            D PGQALAEELA RLGK RCW V WP K +   FKDANE+L+  G   LK+AI +A+ Y
Sbjct: 278 GDGPGQALAEELARRLGKERCWLVKWPKKSEDEHFKDANEVLMSKGPHLLKEAILNAEPY 335

Query: 377 QL 378
            L
Sbjct: 338 PL 335

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BLAST of Csor.00g297240 vs. NCBI nr
Match: KAG6573428.1 (Primase-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 806 bits (2081), Expect = 1.53e-294
Identity = 396/396 (100.00%), Postives = 396/396 (100.00%), Query Frame = 0


Query: 1   MAASILLPIVLQIRRIQMPMKTPFNFNFNHRPAIFCHPLNNFSFSSPSLPLYPTRLLCAL 60
           MAASILLPIVLQIRRIQMPMKTPFNFNFNHRPAIFCHPLNNFSFSSPSLPLYPTRLLCAL
Sbjct: 1   MAASILLPIVLQIRRIQMPMKTPFNFNFNHRPAIFCHPLNNFSFSSPSLPLYPTRLLCAL 60

Query: 61  SPTPPSPSPSPPPVKVLMEKMDLIGIVCDEQCTPGKYYCLLCPKCKGGRLMERSLSLHVI 120
           SPTPPSPSPSPPPVKVLMEKMDLIGIVCDEQCTPGKYYCLLCPKCKGGRLMERSLSLHVI
Sbjct: 61  SPTPPSPSPSPPPVKVLMEKMDLIGIVCDEQCTPGKYYCLLCPKCKGGRLMERSLSLHVI 120

Query: 121 PTGDFAMWRCFQSECGWAGRIFADGRLAFNEFSRTAKLFGGTAKKSLILEPLGDELITYF 180
           PTGDFAMWRCFQSECGWAGRIFADGRLAFNEFSRTAKLFGGTAKKSLILEPLGDELITYF
Sbjct: 121 PTGDFAMWRCFQSECGWAGRIFADGRLAFNEFSRTAKLFGGTAKKSLILEPLGDELITYF 180

Query: 181 SKRMISQETLKRNVVMQTADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGI 240
           SKRMISQETLKRNVVMQTADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGI
Sbjct: 181 SKRMISQETLKRNVVMQTADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGI 240

Query: 241 DDINDADELIIVEGEMDKLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLWSC 300
           DDINDADELIIVEGEMDKLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLWSC
Sbjct: 241 DDINDADELIIVEGEMDKLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLWSC 300

Query: 301 KDLLDKVPRIILATDSDEPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILVQL 360
           KDLLDKVPRIILATDSDEPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILVQL
Sbjct: 301 KDLLDKVPRIILATDSDEPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILVQL 360

Query: 361 GADALKKAIQDAKSYQLCDTDEIQVEEDLESFHEPS 396
           GADALKKAIQDAKSYQLCDTDEIQVEEDLESFHEPS
Sbjct: 361 GADALKKAIQDAKSYQLCDTDEIQVEEDLESFHEPS 396

[Close]

BLAST of Csor.00g297240 vs. NCBI nr
Match: XP_022955059.1 (primase homolog protein-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 790 bits (2040), Expect = 2.53e-288
Identity = 391/396 (98.74%), Postives = 392/396 (98.99%), Query Frame = 0


Query: 1   MAASILLPIVLQIRRIQMPMKTPFNFNFNHRPAIFCHPLNNFSFSSPSLPLYPTRLLCAL 60
           MAASILLPIVLQIRRIQMPMKTPFNFNFNHRPAIF HPLNNFSFSSPSLPLYPTRL CAL
Sbjct: 1   MAASILLPIVLQIRRIQMPMKTPFNFNFNHRPAIFSHPLNNFSFSSPSLPLYPTRLSCAL 60

Query: 61  SPTPPSPSPSPPPVKVLMEKMDLIGIVCDEQCTPGKYYCLLCPKCKGGRLMERSLSLHVI 120
           SPTPPS  PSPPPVKVLMEKMDLIGIVCDEQCTPGKYYCLLCPKCKGGRLMERSLSLHVI
Sbjct: 61  SPTPPS--PSPPPVKVLMEKMDLIGIVCDEQCTPGKYYCLLCPKCKGGRLMERSLSLHVI 120

Query: 121 PTGDFAMWRCFQSECGWAGRIFADGRLAFNEFSRTAKLFGGTAKKSLILEPLGDELITYF 180
           PTGDFAMWRCFQSECGWAGRIFADGRLAFNEFSRTAKLFGGTAKKSLILEPLGDELITYF
Sbjct: 121 PTGDFAMWRCFQSECGWAGRIFADGRLAFNEFSRTAKLFGGTAKKSLILEPLGDELITYF 180

Query: 181 SKRMISQETLKRNVVMQTADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGI 240
           SKRMISQETLKRNVVMQTADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGI
Sbjct: 181 SKRMISQETLKRNVVMQTADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGI 240

Query: 241 DDINDADELIIVEGEMDKLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLWSC 300
           DDINDADELIIVEGEMDKLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLWSC
Sbjct: 241 DDINDADELIIVEGEMDKLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLWSC 300

Query: 301 KDLLDKVPRIILATDSDEPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILVQL 360
           KDLLDKVPRIILATDSDEPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILVQL
Sbjct: 301 KDLLDKVPRIILATDSDEPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILVQL 360

Query: 361 GADALKKAIQDAKSYQLCDTDEIQVEEDLESFHEPS 396
           GADALKKAI+DAKSYQLCDTDEIQVEEDLESFHEPS
Sbjct: 361 GADALKKAIEDAKSYQLCDTDEIQVEEDLESFHEPS 394

[Close]

BLAST of Csor.00g297240 vs. NCBI nr
Match: XP_023541322.1 (primase homolog protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 782 bits (2019), Expect = 4.68e-285
Identity = 386/398 (96.98%), Postives = 390/398 (97.99%), Query Frame = 0


Query: 1   MAASILLPIVLQIRRIQMPMKTPFNFNFNHRPAIFCHPLNNFSFSSPSLPLYPTRLLCAL 60
           MAASILLPIVLQIRRIQMPMKTPFNFN NHRPA+F HPLNNFSFSSPSLPLYPTRL CAL
Sbjct: 1   MAASILLPIVLQIRRIQMPMKTPFNFNINHRPALFSHPLNNFSFSSPSLPLYPTRLSCAL 60

Query: 61  SPTPPSPSPSP--PPVKVLMEKMDLIGIVCDEQCTPGKYYCLLCPKCKGGRLMERSLSLH 120
           SPTPPSPSPSP  PPVKVLMEKMDLIGIVCDE CTPGKYYCLLCPKCKGGRLMERSLSLH
Sbjct: 61  SPTPPSPSPSPSPPPVKVLMEKMDLIGIVCDEHCTPGKYYCLLCPKCKGGRLMERSLSLH 120

Query: 121 VIPTGDFAMWRCFQSECGWAGRIFADGRLAFNEFSRTAKLFGGTAKKSLILEPLGDELIT 180
           VIPTGDFAMWRCFQSECGWAGRIFADGRLAFNEFSRTAKLFGGT KKSLILEPLGDELIT
Sbjct: 121 VIPTGDFAMWRCFQSECGWAGRIFADGRLAFNEFSRTAKLFGGTVKKSLILEPLGDELIT 180

Query: 181 YFSKRMISQETLKRNVVMQTADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFY 240
           YFSKRMISQETLKRNVVMQTADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFY
Sbjct: 181 YFSKRMISQETLKRNVVMQTADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFY 240

Query: 241 GIDDINDADELIIVEGEMDKLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLW 300
           GIDDINDADELIIVEGEMDKLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLW
Sbjct: 241 GIDDINDADELIIVEGEMDKLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLW 300

Query: 301 SCKDLLDKVPRIILATDSDEPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILV 360
           SCKDLLDKVPRIILATDSDEPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEIL+
Sbjct: 301 SCKDLLDKVPRIILATDSDEPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILM 360

Query: 361 QLGADALKKAIQDAKSYQLCDTDEIQVEEDLESFHEPS 396
           QLGADALKKAI+DAKSYQLCDT +IQVEEDLESFHEPS
Sbjct: 361 QLGADALKKAIEDAKSYQLCDTHQIQVEEDLESFHEPS 398

[Close]

BLAST of Csor.00g297240 vs. NCBI nr
Match: XP_022994814.1 (primase homolog protein isoform X1 [Cucurbita maxima])

HSP 1 Score: 781 bits (2017), Expect = 1.49e-284
Identity = 386/398 (96.98%), Postives = 389/398 (97.74%), Query Frame = 0


Query: 1   MAASILLPIVLQIRRIQMPMKTPFNFNFNHRPAIFCHPLNNFSFSSPSLPLYPTRLLCAL 60
           MAASILLPIVLQIRRIQMPMKTPFNFNFNHRPAIF HPLNNFSFSSPSLPLYPTRL CAL
Sbjct: 13  MAASILLPIVLQIRRIQMPMKTPFNFNFNHRPAIFSHPLNNFSFSSPSLPLYPTRLSCAL 72

Query: 61  SPTPPSPSPSP--PPVKVLMEKMDLIGIVCDEQCTPGKYYCLLCPKCKGGRLMERSLSLH 120
           SPTPPSPSPSP  PPVKVLMEKMDLIGIVCDEQCTPGKYYCLLCPKCKGGRLMERSLSLH
Sbjct: 73  SPTPPSPSPSPSAPPVKVLMEKMDLIGIVCDEQCTPGKYYCLLCPKCKGGRLMERSLSLH 132

Query: 121 VIPTGDFAMWRCFQSECGWAGRIFADGRLAFNEFSRTAKLFGGTAKKSLILEPLGDELIT 180
           VIPTGDFAMWRCFQSECGWAGRIFADGRLAFNEFSRTAKLFGGT KKSLILEPLGDELIT
Sbjct: 133 VIPTGDFAMWRCFQSECGWAGRIFADGRLAFNEFSRTAKLFGGTVKKSLILEPLGDELIT 192

Query: 181 YFSKRMISQETLKRNVVMQTADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFY 240
           YFSKRMISQETLKRNVVMQTA RQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFY
Sbjct: 193 YFSKRMISQETLKRNVVMQTAGRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFY 252

Query: 241 GIDDINDADELIIVEGEMDKLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLW 300
           GIDDINDADELIIVEGEMDKLSVEEAGFLNCISVPGGAP KVSTNAVASIQKDTAYQYLW
Sbjct: 253 GIDDINDADELIIVEGEMDKLSVEEAGFLNCISVPGGAPNKVSTNAVASIQKDTAYQYLW 312

Query: 301 SCKDLLDKVPRIILATDSDEPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILV 360
           SCKDLLDKVPR+ILATDSDEPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEIL+
Sbjct: 313 SCKDLLDKVPRVILATDSDEPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILM 372

Query: 361 QLGADALKKAIQDAKSYQLCDTDEIQVEEDLESFHEPS 396
           QLGADALKKAI+DAKSYQLCDTD IQVE DLESFHEPS
Sbjct: 373 QLGADALKKAIEDAKSYQLCDTDHIQVEGDLESFHEPS 410

[Close]

BLAST of Csor.00g297240 vs. NCBI nr
Match: XP_022955060.1 (primase homolog protein-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 655 bits (1689), Expect = 4.68e-236
Identity = 318/319 (99.69%), Postives = 319/319 (100.00%), Query Frame = 0


Query: 78  MEKMDLIGIVCDEQCTPGKYYCLLCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQSECGW 137
           MEKMDLIGIVCDEQCTPGKYYCLLCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQSECGW
Sbjct: 1   MEKMDLIGIVCDEQCTPGKYYCLLCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQSECGW 60

Query: 138 AGRIFADGRLAFNEFSRTAKLFGGTAKKSLILEPLGDELITYFSKRMISQETLKRNVVMQ 197
           AGRIFADGRLAFNEFSRTAKLFGGTAKKSLILEPLGDELITYFSKRMISQETLKRNVVMQ
Sbjct: 61  AGRIFADGRLAFNEFSRTAKLFGGTAKKSLILEPLGDELITYFSKRMISQETLKRNVVMQ 120

Query: 198 TADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGIDDINDADELIIVEGEMD 257
           TADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGIDDINDADELIIVEGEMD
Sbjct: 121 TADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGIDDINDADELIIVEGEMD 180

Query: 258 KLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLWSCKDLLDKVPRIILATDSD 317
           KLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLWSCKDLLDKVPRIILATDSD
Sbjct: 181 KLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLWSCKDLLDKVPRIILATDSD 240

Query: 318 EPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILVQLGADALKKAIQDAKSYQL 377
           EPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILVQLGADALKKAI+DAKSYQL
Sbjct: 241 EPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILVQLGADALKKAIEDAKSYQL 300

Query: 378 CDTDEIQVEEDLESFHEPS 396
           CDTDEIQVEEDLESFHEPS
Sbjct: 301 CDTDEIQVEEDLESFHEPS 319

[Close]

BLAST of Csor.00g297240 vs. ExPASy TrEMBL
Match: A0A6J1GUU9 (primase homolog protein-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111457136 PE=4 SV=1)

HSP 1 Score: 790 bits (2040), Expect = 1.22e-288
Identity = 391/396 (98.74%), Postives = 392/396 (98.99%), Query Frame = 0


Query: 1   MAASILLPIVLQIRRIQMPMKTPFNFNFNHRPAIFCHPLNNFSFSSPSLPLYPTRLLCAL 60
           MAASILLPIVLQIRRIQMPMKTPFNFNFNHRPAIF HPLNNFSFSSPSLPLYPTRL CAL
Sbjct: 1   MAASILLPIVLQIRRIQMPMKTPFNFNFNHRPAIFSHPLNNFSFSSPSLPLYPTRLSCAL 60

Query: 61  SPTPPSPSPSPPPVKVLMEKMDLIGIVCDEQCTPGKYYCLLCPKCKGGRLMERSLSLHVI 120
           SPTPPS  PSPPPVKVLMEKMDLIGIVCDEQCTPGKYYCLLCPKCKGGRLMERSLSLHVI
Sbjct: 61  SPTPPS--PSPPPVKVLMEKMDLIGIVCDEQCTPGKYYCLLCPKCKGGRLMERSLSLHVI 120

Query: 121 PTGDFAMWRCFQSECGWAGRIFADGRLAFNEFSRTAKLFGGTAKKSLILEPLGDELITYF 180
           PTGDFAMWRCFQSECGWAGRIFADGRLAFNEFSRTAKLFGGTAKKSLILEPLGDELITYF
Sbjct: 121 PTGDFAMWRCFQSECGWAGRIFADGRLAFNEFSRTAKLFGGTAKKSLILEPLGDELITYF 180

Query: 181 SKRMISQETLKRNVVMQTADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGI 240
           SKRMISQETLKRNVVMQTADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGI
Sbjct: 181 SKRMISQETLKRNVVMQTADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGI 240

Query: 241 DDINDADELIIVEGEMDKLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLWSC 300
           DDINDADELIIVEGEMDKLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLWSC
Sbjct: 241 DDINDADELIIVEGEMDKLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLWSC 300

Query: 301 KDLLDKVPRIILATDSDEPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILVQL 360
           KDLLDKVPRIILATDSDEPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILVQL
Sbjct: 301 KDLLDKVPRIILATDSDEPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILVQL 360

Query: 361 GADALKKAIQDAKSYQLCDTDEIQVEEDLESFHEPS 396
           GADALKKAI+DAKSYQLCDTDEIQVEEDLESFHEPS
Sbjct: 361 GADALKKAIEDAKSYQLCDTDEIQVEEDLESFHEPS 394

[Close]

BLAST of Csor.00g297240 vs. ExPASy TrEMBL
Match: A0A6J1K3X3 (primase homolog protein isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490430 PE=4 SV=1)

HSP 1 Score: 781 bits (2017), Expect = 7.19e-285
Identity = 386/398 (96.98%), Postives = 389/398 (97.74%), Query Frame = 0


Query: 1   MAASILLPIVLQIRRIQMPMKTPFNFNFNHRPAIFCHPLNNFSFSSPSLPLYPTRLLCAL 60
           MAASILLPIVLQIRRIQMPMKTPFNFNFNHRPAIF HPLNNFSFSSPSLPLYPTRL CAL
Sbjct: 13  MAASILLPIVLQIRRIQMPMKTPFNFNFNHRPAIFSHPLNNFSFSSPSLPLYPTRLSCAL 72

Query: 61  SPTPPSPSPSP--PPVKVLMEKMDLIGIVCDEQCTPGKYYCLLCPKCKGGRLMERSLSLH 120
           SPTPPSPSPSP  PPVKVLMEKMDLIGIVCDEQCTPGKYYCLLCPKCKGGRLMERSLSLH
Sbjct: 73  SPTPPSPSPSPSAPPVKVLMEKMDLIGIVCDEQCTPGKYYCLLCPKCKGGRLMERSLSLH 132

Query: 121 VIPTGDFAMWRCFQSECGWAGRIFADGRLAFNEFSRTAKLFGGTAKKSLILEPLGDELIT 180
           VIPTGDFAMWRCFQSECGWAGRIFADGRLAFNEFSRTAKLFGGT KKSLILEPLGDELIT
Sbjct: 133 VIPTGDFAMWRCFQSECGWAGRIFADGRLAFNEFSRTAKLFGGTVKKSLILEPLGDELIT 192

Query: 181 YFSKRMISQETLKRNVVMQTADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFY 240
           YFSKRMISQETLKRNVVMQTA RQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFY
Sbjct: 193 YFSKRMISQETLKRNVVMQTAGRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFY 252

Query: 241 GIDDINDADELIIVEGEMDKLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLW 300
           GIDDINDADELIIVEGEMDKLSVEEAGFLNCISVPGGAP KVSTNAVASIQKDTAYQYLW
Sbjct: 253 GIDDINDADELIIVEGEMDKLSVEEAGFLNCISVPGGAPNKVSTNAVASIQKDTAYQYLW 312

Query: 301 SCKDLLDKVPRIILATDSDEPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILV 360
           SCKDLLDKVPR+ILATDSDEPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEIL+
Sbjct: 313 SCKDLLDKVPRVILATDSDEPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILM 372

Query: 361 QLGADALKKAIQDAKSYQLCDTDEIQVEEDLESFHEPS 396
           QLGADALKKAI+DAKSYQLCDTD IQVE DLESFHEPS
Sbjct: 373 QLGADALKKAIEDAKSYQLCDTDHIQVEGDLESFHEPS 410

[Close]

BLAST of Csor.00g297240 vs. ExPASy TrEMBL
Match: A0A6J1GU46 (primase homolog protein-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111457136 PE=4 SV=1)

HSP 1 Score: 655 bits (1689), Expect = 2.27e-236
Identity = 318/319 (99.69%), Postives = 319/319 (100.00%), Query Frame = 0


Query: 78  MEKMDLIGIVCDEQCTPGKYYCLLCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQSECGW 137
           MEKMDLIGIVCDEQCTPGKYYCLLCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQSECGW
Sbjct: 1   MEKMDLIGIVCDEQCTPGKYYCLLCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQSECGW 60

Query: 138 AGRIFADGRLAFNEFSRTAKLFGGTAKKSLILEPLGDELITYFSKRMISQETLKRNVVMQ 197
           AGRIFADGRLAFNEFSRTAKLFGGTAKKSLILEPLGDELITYFSKRMISQETLKRNVVMQ
Sbjct: 61  AGRIFADGRLAFNEFSRTAKLFGGTAKKSLILEPLGDELITYFSKRMISQETLKRNVVMQ 120

Query: 198 TADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGIDDINDADELIIVEGEMD 257
           TADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGIDDINDADELIIVEGEMD
Sbjct: 121 TADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGIDDINDADELIIVEGEMD 180

Query: 258 KLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLWSCKDLLDKVPRIILATDSD 317
           KLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLWSCKDLLDKVPRIILATDSD
Sbjct: 181 KLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLWSCKDLLDKVPRIILATDSD 240

Query: 318 EPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILVQLGADALKKAIQDAKSYQL 377
           EPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILVQLGADALKKAI+DAKSYQL
Sbjct: 241 EPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILVQLGADALKKAIEDAKSYQL 300

Query: 378 CDTDEIQVEEDLESFHEPS 396
           CDTDEIQVEEDLESFHEPS
Sbjct: 301 CDTDEIQVEEDLESFHEPS 319

[Close]

BLAST of Csor.00g297240 vs. ExPASy TrEMBL
Match: A0A6J1K2E1 (primase homolog protein isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111490430 PE=4 SV=1)

HSP 1 Score: 642 bits (1657), Expect = 1.70e-231
Identity = 311/319 (97.49%), Postives = 314/319 (98.43%), Query Frame = 0


Query: 78  MEKMDLIGIVCDEQCTPGKYYCLLCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQSECGW 137
           MEKMDLIGIVCDEQCTPGKYYCLLCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQSECGW
Sbjct: 1   MEKMDLIGIVCDEQCTPGKYYCLLCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQSECGW 60

Query: 138 AGRIFADGRLAFNEFSRTAKLFGGTAKKSLILEPLGDELITYFSKRMISQETLKRNVVMQ 197
           AGRIFADGRLAFNEFSRTAKLFGGT KKSLILEPLGDELITYFSKRMISQETLKRNVVMQ
Sbjct: 61  AGRIFADGRLAFNEFSRTAKLFGGTVKKSLILEPLGDELITYFSKRMISQETLKRNVVMQ 120

Query: 198 TADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGIDDINDADELIIVEGEMD 257
           TA RQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGIDDINDADELIIVEGEMD
Sbjct: 121 TAGRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGIDDINDADELIIVEGEMD 180

Query: 258 KLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLWSCKDLLDKVPRIILATDSD 317
           KLSVEEAGFLNCISVPGGAP KVSTNAVASIQKDTAYQYLWSCKDLLDKVPR+ILATDSD
Sbjct: 181 KLSVEEAGFLNCISVPGGAPNKVSTNAVASIQKDTAYQYLWSCKDLLDKVPRVILATDSD 240

Query: 318 EPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILVQLGADALKKAIQDAKSYQL 377
           EPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEIL+QLGADALKKAI+DAKSYQL
Sbjct: 241 EPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILMQLGADALKKAIEDAKSYQL 300

Query: 378 CDTDEIQVEEDLESFHEPS 396
           CDTD IQVE DLESFHEPS
Sbjct: 301 CDTDHIQVEGDLESFHEPS 319

[Close]

BLAST of Csor.00g297240 vs. ExPASy TrEMBL
Match: A0A6J1CNI0 (primase homolog protein isoform X1 OS=Momordica charantia OX=3673 GN=LOC111012634 PE=4 SV=1)

HSP 1 Score: 579 bits (1493), Expect = 2.65e-205
Identity = 292/381 (76.64%), Postives = 318/381 (83.46%), Query Frame = 0


Query: 6   LLPIVLQIRRIQMPMKTPFNFNFNHRPAIFCHPLNNFSFSSPS-LPLYPTRLLCALSPTP 65
           ++P  LQIR  +   + P N  FNHRPA   HPLN FSFSSP  L    TR   A S  P
Sbjct: 17  IVPFFLQIRIQRGINQMPINAAFNHRPAPLFHPLNPFSFSSPPFLRFCSTRPSSAASHAP 76

Query: 66  PSPSPSPPPVKVLMEKMDLIGIVCDEQCTPGKYYCLLCPKCKGGRLMERSLSLHVIPTGD 125
           P   PSPPPVKVLMEKMDLIGI CDE CTPGKYYCL CPKCKGG L ERSLSLHV+PTGD
Sbjct: 77  PP--PSPPPVKVLMEKMDLIGIACDENCTPGKYYCLFCPKCKGGPLTERSLSLHVVPTGD 136

Query: 126 FAMWRCFQSECGWAGRIFADGRLAFNEFSRTAKLFGGTAKKSLILEPLGDELITYFSKRM 185
           FAMWRC+ SECGWAGRIFADGRLAFN   RT KL G +AK+SL+LEPLGDELI YFSKRM
Sbjct: 137 FAMWRCYLSECGWAGRIFADGRLAFNGLGRTVKLVGRSAKESLVLEPLGDELIFYFSKRM 196

Query: 186 ISQETLKRNVVMQTADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGIDDIN 245
           I QETL+RN VMQ A RQ  IAFTYRQNGQL+GCKYRT++K+FWQ KGTEKL YGIDDIN
Sbjct: 197 IPQETLERNFVMQIAGRQGVIAFTYRQNGQLLGCKYRTLDKRFWQEKGTEKLLYGIDDIN 256

Query: 246 DADELIIVEGEMDKLSVEEAGFLNCISVPGGAPTKVSTNAVA-SIQKDTAYQYLWSCKDL 305
           DADE+IIVEGEMDKLSVEEAGFLNCISVPGGAP KVST++V  SI+KDTAYQYLWSCKD 
Sbjct: 257 DADEVIIVEGEMDKLSVEEAGFLNCISVPGGAPRKVSTDSVLPSIEKDTAYQYLWSCKDY 316

Query: 306 LDKVPRIILATDSDEPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILVQLGAD 365
           LDKV  IILATDSDEPGQALAEELA RLGKHRCWRVDWPYKDDFNRFKDANEILV LG D
Sbjct: 317 LDKVSHIILATDSDEPGQALAEELARRLGKHRCWRVDWPYKDDFNRFKDANEILVHLGPD 376

Query: 366 ALKKAIQDAKSYQLCDTDEIQ 384
           ALK+A+++AKSY+  + ++IQ
Sbjct: 377 ALKRALKNAKSYEHSNANQIQ 395

[Close]

BLAST of Csor.00g297240 vs. TAIR 10
Match: AT1G30680.1 (toprim domain-containing protein )

HSP 1 Score: 306.2 bits (783), Expect = 3.8e-83
Identity = 155/313 (49.52%), Postives = 201/313 (64.22%), Query Frame = 0


Query: 90  EQCTPGKYYCLLCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQSECGWAGRIFADGRLAF 149
           E C PG++  L+CP C+GG   E+SLSL + P G  A W CF+ +CG  G + ADG LA 
Sbjct: 124 ENCPPGQHSGLICPTCEGGNSGEKSLSLFIAPDGSSATWNCFRGKCGLKGGVRADGGLA- 183

Query: 150 NEFSRTAKLFGGTAKKSLILEPLGDELITYFSKRMISQETLKRNVVMQ-TADRQAAIAFT 209
                  K+      + + LEPL DE+  YF+ R IS++TL+RN VMQ     +  IAFT
Sbjct: 184 -SADPIEKVERKITVEGIELEPLCDEIQDYFAARAISRKTLERNRVMQKRIGDEIVIAFT 243

Query: 210 YRQNGQLVGCKYRTINKKFWQAKGTEKLFYGIDDINDADELIIVEGEMDKLSVEEAGFLN 269
           Y Q G+LV CKYR++ K F+Q + T ++ YG+DDI    E+IIVEGE+DKL++EEAGFLN
Sbjct: 244 YWQRGELVSCKYRSLTKMFFQERKTRRILYGLDDIEKTSEVIIVEGEIDKLAMEEAGFLN 303

Query: 270 CISVPGGAPTKVSTNAVASIQKDTAYQYLWSCKDLLDKVPRIILATDSDEPGQALAEELA 329
           C+SVP GAP KVS+  + S  KDT Y++LW+C D L K  RI++ATD D PGQA+AEE+A
Sbjct: 304 CVSVPDGAPAKVSSKEIPSEDKDTKYKFLWNCNDYLKKASRIVIATDGDGPGQAMAEEIA 363

Query: 330 LRLGKHRCWRVDWPYKDDFNRFKDANEILVQLGADALKKAIQDAKSYQLCDT-------D 389
            RLGK RCWRV WP K +   FKDANE+L+  G   LK+AI DA+ Y +          D
Sbjct: 364 RRLGKERCWRVKWPKKSEDEHFKDANEVLMSKGPHLLKEAILDAEPYPILGLFSFKDFFD 423

Query: 390 EIQVEEDLESFHE 395
           EI    D    HE
Sbjct: 424 EIDAYYDRTHGHE 434

[Close]

BLAST of Csor.00g297240 vs. TAIR 10
Match: AT1G30660.1 (nucleic acid binding;nucleic acid binding )

HSP 1 Score: 299.7 bits (766), Expect = 3.5e-81
Identity = 152/302 (50.33%), Postives = 199/302 (65.89%), Query Frame = 0


Query: 77  LMEKMDLIGIVCDEQCTPGKYYCLLCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQSECG 136
           LM K+   GI   + C PG   CL+CPKC+ G   E+SL+L++ P G  A W C + +CG
Sbjct: 38  LMRKLSEQGIDA-QNCPPGVRSCLICPKCEVGDSGEKSLTLYIYPDGSSAKWTC-RRKCG 97

Query: 137 WAGRIFADGRLAFNEFSRTAKLFGGTAKKSLILEPLGDELITYFSKRMISQETLKRNVVM 196
             G +  DG+L   +     K+      +S+ LEPL DE+  +F+ R IS +TL+RN VM
Sbjct: 98  LKGVLQVDGKLVSKD--PIGKVERKITVESIKLEPLCDEIQDFFAARAISGKTLERNRVM 157

Query: 197 Q-TADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGIDDINDADELIIVEGE 256
           Q   D +  IAFTY Q G+LV CKYR++ KKF Q + T K+ YG+DDI +  E+IIVEGE
Sbjct: 158 QKRIDDEIVIAFTYWQRGELVSCKYRSLTKKFVQERNTRKILYGLDDIEETSEIIIVEGE 217

Query: 257 MDKLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLWSCKDLLDKVPRIILATD 316
            DKL++EEAGF NC+SVP GAP  VS+  + S  KDTA++Y+W+C D L K  RI++ATD
Sbjct: 218 PDKLAMEEAGFFNCVSVPDGAPETVSSKEIPSESKDTAFKYIWNCNDYLKKASRIVIATD 277

Query: 317 SDEPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILVQLGADALKKAIQDAKSY 376
            D PGQALAEELA RLGK RCW V WP K +   FKDANE+L+  G   LK+AI +A+ Y
Sbjct: 278 GDGPGQALAEELARRLGKERCWLVKWPKKSEDEHFKDANEVLMSKGPHLLKEAILNAEPY 335

Query: 377 QL 378
            L
Sbjct: 338 PL 335

The following BLAST results are available for this feature:

BLAST of Csor.00g297240 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B5X582	5.3e-82	49.52	Twinkle homolog protein, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=...	[more]
F4I6E6	5.0e-80	50.33	Primase homolog protein OS=Arabidopsis thaliana OX=3702 GN=At1g30660 PE=3 SV=1	[more]

BLAST of Csor.00g297240 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6573428.1	1.53e-294	100.00	Primase-like protein, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022955059.1	2.53e-288	98.74	primase homolog protein-like isoform X1 [Cucurbita moschata]	[more]
XP_023541322.1	4.68e-285	96.98	primase homolog protein-like [Cucurbita pepo subsp. pepo]	[more]
XP_022994814.1	1.49e-284	96.98	primase homolog protein isoform X1 [Cucurbita maxima]	[more]
XP_022955060.1	4.68e-236	99.69	primase homolog protein-like isoform X2 [Cucurbita moschata]	[more]

BLAST of Csor.00g297240 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GUU9	1.22e-288	98.74	primase homolog protein-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1K3X3	7.19e-285	96.98	primase homolog protein isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490430 P...	[more]
A0A6J1GU46	2.27e-236	99.69	primase homolog protein-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1K2E1	1.70e-231	97.49	primase homolog protein isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111490430 P...	[more]
A0A6J1CNI0	2.65e-205	76.64	primase homolog protein isoform X1 OS=Momordica charantia OX=3673 GN=LOC11101263...	[more]

BLAST of Csor.00g297240 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G30680.1	3.8e-83	49.52	toprim domain-containing protein	[more]
AT1G30660.1	3.5e-81	50.33	nucleic acid binding;nucleic acid binding	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006171	TOPRIM domain	SMART	SM00493	toprim5	coord: 247..336 e-value: 2.1E-6 score: 37.3
IPR006171	TOPRIM domain	PFAM	PF13662	Toprim_4	coord: 247..339 e-value: 6.5E-18 score: 65.0
IPR006171	TOPRIM domain	PROSITE	PS50880	TOPRIM	coord: 247..358 score: 9.558442
None	No IPR available	GENE3D	3.40.1360.10		coord: 234..376 e-value: 1.1E-31 score: 111.4
None	No IPR available	PANTHER	PTHR12873:SF1	ACID-BINDING PROTEIN, PUTATIVE-RELATED	coord: 74..376
None	No IPR available	SUPERFAMILY	56731	DNA primase core	coord: 179..357
IPR027032	Twinkle-like protein	PANTHER	PTHR12873	T7-LIKE MITOCHONDRIAL DNA HELICASE	coord: 74..376
IPR034154	Archaeal primase DnaG/twinkle, TOPRIM domain	CDD	cd01029	TOPRIM_primases	coord: 247..339 e-value: 2.00289E-11 score: 57.6654

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g297240.m01	Csor.00g297240.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032508	DNA duplex unwinding
molecular_function	GO:0043139	5'-3' DNA helicase activity
molecular_function	GO:0003697	single-stranded DNA binding