Csor.00g262580 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g262580
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionProtein of unknown function (DUF803)
LocationCsor_Chr11: 12006554 .. 12011093 (-)
RNA-Seq ExpressionCsor.00g262580
SyntenyCsor.00g262580
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGGAGTGGGTTATTGGAGCTTTCATCAATCTCTTTGGCAGCCTTGCCATCAACTTTGGGACGAACCTTCTTAAATTAGGCCATAACGAGGTTTGCAGAGATCCCTTTTCCTTTTTTTTTTTGTTTGGTTTCTCTATGATTTGCTCTTGGTCTACACTGTCTAGCTAAGTTCTCTGCAACTACTTGTTGTTGCCGTTCTTTGTAGACAGCATTTCGTTTACACATTTTTCTTGAGGATGCAATACAGGCATGCTCTAAGAGCTTTAGTGGTAGGTTTTAGTGTGTCATAAATCATAAAACATAAATGTCATGTATTCTTCCAAAAAAAAGAAAAAAAAAAGTTTTACTTCAAAGTGGTAAACCCGTTGCCCTTTTTGGCTTGGTAAGTGATAGCCCGCCAAACTTTTAAGTTGGATAGCTAGTTGAGCTCAATTATACTCCACTCGATCACCCTTTTTTGGCATTCATTGTTTCATGCTACAGTCTTGTTATTTGTTACATGTCCCCTGGTTTTAAAAATACCCTTGCAGAGAGAGAAGCATTCTATACTTGAAAACAATGGTTCTAATGGAAAGGCTCCAATGAAGCCCATAATATACTTTCAGGCTTGGAGAGTTGGTAAGTCATTTTCTGGTGCTCAATTCATTATAGGCATTTAAATGTTAAACTTTTTTGGAAGAAGAGTACATTTGAAACCTAATTCCTTATTTATAACTTGAAAGCTTTGCTGTACAGGTATTACATTTTTCATTCTTGGAAATTGCCTTAATTTTATTTCCTTCGGATATGCTGCCCAGGTTAGTAGAAATTACTTTATCCCATTTCACAACGTGAATATATTGGTATTGTAGGGATATGTGAATTTTCGTTATTTGCAATGTTTATTAGATGTTTTACTTCTCTACTGGTCGTGTATTTCCTGTTCCACTTTTTTCTAATTTTATTTTCTTCCCCCTTCTCCCCCCTGCCCCAGTCTCTTCTTGCAGCACTTGGATCTGTTCAGTTTGTGTCCAATATTGCTTTTGCATACTTCGTACTCCACAAGATGGTTACTGTTAAGTATGTTGACACCTTTAGTCATACATGTCTTTGGAACCATGAAGGCTGAGCTAACGGGTTTTCATCATCTTTATTTCTAATTTAATGGTCAATGATTGCATTGCTTCTTTTGCAGGGTAATGGTTGCCACTGCTTTTATTGTTCTTGGAAATGTTTTTCTAGTTGCTTTTGGCAACCATCAGTCCCCTGGTAGATGTTTTACTTTTGATTTATATATCTTTCAAGTTCTATTGTCTTTTCCTTCTAATACGAAATGTCAAGAATGTCTATTTTGAGTTTTCCATGAAGCTTCTATGATATGGAGGAAATGATCATTTAATAATTATCTCAGAGCGTCATCTATTAAATTTTTTACCGATAGGCCATTGTTGGAACTTGGCCTATATCAGCAAGTATGCTTAAAAGATGATGATTCAAACCCCCATAAAAAAATTAGAAGTCAGCTGCCCAATATCATGAAACTTGCATTATGATGGTGCTTTATTTTTGTTCAGCACTTCTGACCATTCAGGATCACTTATCCTGTCTGGGAAAAAATTGATTAAGAATGCTTTAACGTTTAGTGTTGGTTACAGTTTCTGCTTACCATTTCTTCCATAGTTGATGCCTTCTACTTTCGAGTTTTATGAATAAAATGGTCAAATATTTTTTTTTTATCTTTTTGACTGCTTATGTTGATTTTGGTGGATGAATGTCCACATTTCCTTTTGTTCAATGCTATCAATGCATTAAATATGGCAAAATGTACCAATTCTTTGAATGTACATGCTGCATATACTCTTAGCAACAATGCCTTTGGTAGATACTAACTTGTCTTTGAATGCATTTCCTCTGTCAATATGCTGTGTGGTTTAAATACTAACTCTATGGGGTGATATTCAAGTGCCATGAAGTTTTGTTGAACTGTGGCTTAGTAATGGGTTGTTGCCCGCAACATTTTATTTTACCTTGTGAAGCCTACAATCGTTAACAAATTTATAGTAGATTACTAACCAAAGAGGCGTTCTTTCTGACTGCTACTTAGATGAGACAAATTAGGTAAAAAAGGGTTTACAGGGTGGTAGTGCAGAAAATAATTTATGCAGTGGATTATTTGATCTAATCTGCTTGTAAATAATAAATGTTCGTATTTTGTTTTCTCTTTTCAGTGTACACTTCAGAACAGCTGGTGGAAAAATACGGAAACATTACTTTCCTTCTATACTGCGTGATTTTGATCTTGGTTGTTGCTATACATCACTCAATCTATAGGTCAGAGTTAAAATAGAATATTTCTTTCTTACTTAATATGAAGCTTCGTGTTGATAATAGTATCCTGTATGGAACAAACGTGCAGAAGGGGAGAGATGTTACTTTCTGTTTCTGGACAAGATCTCAGACCATATTGGCATATGCTGCTACCATTTTCCTATGCTATAGTTTCAGGTGCAATAGGCTCTTGCTCAGTCTTGTTTGCAAAATCTCTGTAAGTTCGCTGACTTTTTTGTCCCTTTTTTCTGTAGGTGCTGAACCCACTTATATTTCTTTTTAGCTTTCTCTTTCTGATGTCGAGTTATCTGTTTTAGCTCAATACTGCTCCGATTGGCCATGTCTAGTGATTATCAGCTGCATAGCTGGTTTACATACTCCTTACTGCTTTTGTTTCTCAGTACTGCGGGATTTTGGGTAAGGTCATTGTGGGACTGTGCTTTTCCTCTTCCATGTTTTCATATTATTTTACTAATGTAAATGAACATCATATGTTTAACTTTTATTTCTGGATTTTTTTTTATCTATTTCTACAGATGGCCAGGTTGAATGAAGGACTTTCACTGTTTGATGCAATCCTTATTGTTCCCATGTTTCAGATAGCTTGGACCTTCTTCTCCATTTGTACAGGTTTTATATACTTTAAAGAATTCCAGGTTCTCTCTCTCTCCCTCTCTCTCTGCTTGTGTGTGGGTGGTTGTGAGTGCCTTTTTTTTTTTTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAGGAATACATGAAAAGAACCGTGTGCTTGAATGACACTGTTTATGATATACTGAACCTACTAAATAGTATATGCATGTTCTTAGGAAAATGGTTTGTATAGCATTGGATCAAAGCTATGTCAATCGGCTTAGATCATAAATTGTAGTGGTTTCCTGGGGACTAGCAATCTTTTAAACAAGTTTGTAGCATTTTGTGGGCTGAGATGAGTTCTCTGGTTATTACCTTCTTAACTTCTCAGGAATGAGATTCATATTTCTTGTATGAGTCAACAAGTAACCCAGTTATCTTTATTTTTGTCCTCTTTCATGACACAGTAACTATGGTATTGATCATATATTTTGAAAATAGTCCCATTTCACTCTTTGTATTAAGCGGTAAATAAAGTGATAATTGTCTAACCATGCGCTTTTTTAACCTTTGATTTCAATGTCATGCTGCCTGTTGCCCTTAAATTCTGTCCATGCCTCAAGCATGAATTCAGTTTAGGTAATAAGCTTGCCTTTGTATTATCTCTTCTATTCAGGTGCTTGATGCTTTGAGAACGACGATGTTCATACTAGGAATGATGTCGGTTTTCATAGGCATTTCTTTGCTAGCTCCTGATGAGCCTAGAGGTATTTTAATTTAGAATTTAAATTTTGGATTACGTTGCAAATGTGAGAGCTTGATTGGAACATGAATTTCAGACGGTGAGTTCAAAGATAATTCGTTGGTATCCGGAACGTCCACTAGCTTCTCAGCAGAAGAAGAGAGGTACAACAATTTTTTTGCATGCTTATATGACATTATTTTTTCCATTGATTCTGAGTTCAAAACCACTTTCTGTTTGTAATGAAGATTCTGGTACTATACCCTTTTCTTTGTGGGGGCAATGTTAGTTCTAAACTAGAATTTCTCAGCAGTGAGAAGCCAGGGCTATTAAAAATGCGTACTTGTTTCGGTTTATTTTATGGATGTCGTAAAGTATTGTACAATGAAAAATCTATTATAGATGAAGTGCTGGGACTTTCTGCCATTTGATCTGCACCTCCCTCTTTACCCATTCTGTTGCATCTTTTCCATCTGCAGACTAATTGTGTCATCCAAAGAACTAGAGACTAGGGACGCAAGATCATTTTCACAAGGATTCTTTTTAAAGATTACAGATGTAGCAGCCAAGGCGAAGGTATTGGAGTCCAACATTGGTGATTTTAGAAGCTTACTTGGTCGACATGTTTGAACGGTTTAACTATATGCCATCTTATTCTCTTTGCAGGCTGCTTTAGCACTTTCTCTGGGTTTTGGAGAAGATTCACTCAGTGCATCAGCAGTACTTGTGATGCCTATGATGTCATCAAGGATGACTGGCTTTCGAGGAACTGGGTTCGACCGCTCCAAGTTTTTCTCGATGAGGAAGCCTGATTGGAGTAAGATCTCGTTGGATGATGAGGATCCGAAGGTGGTGGTCAACACAAACATAGGTTTCTCCCAAAACCTTTGA

mRNA sequence

ATGGGGGAGTGGGTTATTGGAGCTTTCATCAATCTCTTTGGCAGCCTTGCCATCAACTTTGGGACGAACCTTCTTAAATTAGGCCATAACGAGAGAGAGAAGCATTCTATACTTGAAAACAATGGTTCTAATGGAAAGGCTCCAATGAAGCCCATAATATACTTTCAGGCTTGGAGAGTTGGTATTACATTTTTCATTCTTGGAAATTGCCTTAATTTTATTTCCTTCGGATATGCTGCCCAGTCTCTTCTTGCAGCACTTGGATCTGTTCAGTTTGTGTCCAATATTGCTTTTGCATACTTCGTACTCCACAAGATGGTTACTGTTAAGGTAATGGTTGCCACTGCTTTTATTGTTCTTGGAAATGTTTTTCTAGTTGCTTTTGGCAACCATCAGTCCCCTGTGTACACTTCAGAACAGCTGGTGGAAAAATACGGAAACATTACTTTCCTTCTATACTGCGTGATTTTGATCTTGGTTGTTGCTATACATCACTCAATCTATAGAAGGGGAGAGATGTTACTTTCTGTTTCTGGACAAGATCTCAGACCATATTGGCATATGCTGCTACCATTTTCCTATGCTATAGTTTCAGGTGCAATAGGCTCTTGCTCAGTCTTGTTTGCAAAATCTCTCTCAATACTGCTCCGATTGGCCATGTCTAGTGATTATCAGCTGCATAGCTGGTTTACATACTCCTTACTGCTTTTGTTTCTCAGTACTGCGGGATTTTGGATGGCCAGGTTGAATGAAGGACTTTCACTGTTTGATGCAATCCTTATTGTTCCCATGTTTCAGATAGCTTGGACCTTCTTCTCCATTTGTACAGGTTTTATATACTTTAAAGAATTCCAGGTGCTTGATGCTTTGAGAACGACGATGTTCATACTAGGAATGATGTCGGTTTTCATAGGCATTTCTTTGCTAGCTCCTGATGAGCCTAGAGACGGTGAGTTCAAAGATAATTCGTTGGTATCCGGAACGTCCACTAGCTTCTCAGCAGAAGAAGAGAGACTAATTGTGTCATCCAAAGAACTAGAGACTAGGGACGCAAGATCATTTTCACAAGGATTCTTTTTAAAGATTACAGATGTAGCAGCCAAGGCGAAGGCTGCTTTAGCACTTTCTCTGGGTTTTGGAGAAGATTCACTCAGTGCATCAGCAGTACTTGTGATGCCTATGATGTCATCAAGGATGACTGGCTTTCGAGGAACTGGGTTCGACCGCTCCAAGTTTTTCTCGATGAGGAAGCCTGATTGGAGTAAGATCTCGTTGGATGATGAGGATCCGAAGGTGGTGGTCAACACAAACATAGGTTTCTCCCAAAACCTTTGA

Coding sequence (CDS)

ATGGGGGAGTGGGTTATTGGAGCTTTCATCAATCTCTTTGGCAGCCTTGCCATCAACTTTGGGACGAACCTTCTTAAATTAGGCCATAACGAGAGAGAGAAGCATTCTATACTTGAAAACAATGGTTCTAATGGAAAGGCTCCAATGAAGCCCATAATATACTTTCAGGCTTGGAGAGTTGGTATTACATTTTTCATTCTTGGAAATTGCCTTAATTTTATTTCCTTCGGATATGCTGCCCAGTCTCTTCTTGCAGCACTTGGATCTGTTCAGTTTGTGTCCAATATTGCTTTTGCATACTTCGTACTCCACAAGATGGTTACTGTTAAGGTAATGGTTGCCACTGCTTTTATTGTTCTTGGAAATGTTTTTCTAGTTGCTTTTGGCAACCATCAGTCCCCTGTGTACACTTCAGAACAGCTGGTGGAAAAATACGGAAACATTACTTTCCTTCTATACTGCGTGATTTTGATCTTGGTTGTTGCTATACATCACTCAATCTATAGAAGGGGAGAGATGTTACTTTCTGTTTCTGGACAAGATCTCAGACCATATTGGCATATGCTGCTACCATTTTCCTATGCTATAGTTTCAGGTGCAATAGGCTCTTGCTCAGTCTTGTTTGCAAAATCTCTCTCAATACTGCTCCGATTGGCCATGTCTAGTGATTATCAGCTGCATAGCTGGTTTACATACTCCTTACTGCTTTTGTTTCTCAGTACTGCGGGATTTTGGATGGCCAGGTTGAATGAAGGACTTTCACTGTTTGATGCAATCCTTATTGTTCCCATGTTTCAGATAGCTTGGACCTTCTTCTCCATTTGTACAGGTTTTATATACTTTAAAGAATTCCAGGTGCTTGATGCTTTGAGAACGACGATGTTCATACTAGGAATGATGTCGGTTTTCATAGGCATTTCTTTGCTAGCTCCTGATGAGCCTAGAGACGGTGAGTTCAAAGATAATTCGTTGGTATCCGGAACGTCCACTAGCTTCTCAGCAGAAGAAGAGAGACTAATTGTGTCATCCAAAGAACTAGAGACTAGGGACGCAAGATCATTTTCACAAGGATTCTTTTTAAAGATTACAGATGTAGCAGCCAAGGCGAAGGCTGCTTTAGCACTTTCTCTGGGTTTTGGAGAAGATTCACTCAGTGCATCAGCAGTACTTGTGATGCCTATGATGTCATCAAGGATGACTGGCTTTCGAGGAACTGGGTTCGACCGCTCCAAGTTTTTCTCGATGAGGAAGCCTGATTGGAGTAAGATCTCGTTGGATGATGAGGATCCGAAGGTGGTGGTCAACACAAACATAGGTTTCTCCCAAAACCTTTGA

Protein sequence

MGEWVIGAFINLFGSLAINFGTNLLKLGHNEREKHSILENNGSNGKAPMKPIIYFQAWRVGITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVLGNVFLVAFGNHQSPVYTSEQLVEKYGNITFLLYCVILILVVAIHHSIYRRGEMLLSVSGQDLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLLLLFLSTAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGMMSVFIGISLLAPDEPRDGEFKDNSLVSGTSTSFSAEEERLIVSSKELETRDARSFSQGFFLKITDVAAKAKAALALSLGFGEDSLSASAVLVMPMMSSRMTGFRGTGFDRSKFFSMRKPDWSKISLDDEDPKVVVNTNIGFSQNL
Homology
BLAST of Csor.00g262580 vs. ExPASy Swiss-Prot
Match: Q8RWF4 (Probable magnesium transporter NIPA8 OS=Arabidopsis thaliana OX=3702 GN=At3g26670 PE=2 SV=1)

HSP 1 Score: 606.7 bits (1563), Expect = 2.1e-172
Identity = 316/439 (71.98%), Postives = 370/439 (84.28%), Query Frame = 0

Query: 1   MGEWVIGAFINLFGSLAINFGTNLLKLGHNEREKHSILENNGSNGKAPMKPIIYFQAWRV 60
           MGEWVIGAFIN+FGS+AINFGTNLLKLGHNERE+ ++ +     GK P+KPII+ Q WRV
Sbjct: 1   MGEWVIGAFINIFGSVAINFGTNLLKLGHNERERLALQD---GGGKMPLKPIIHNQTWRV 60

Query: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120
           GI  F+LGNCLNFISFGYAAQSLLAALGS+QFVSNIAFAY VL+KMVTVKV+VATAFIVL
Sbjct: 61  GILVFLLGNCLNFISFGYAAQSLLAALGSIQFVSNIAFAYVVLNKMVTVKVLVATAFIVL 120

Query: 121 GNVFLVAFGNHQSPVYTSEQLVEKYGNITFLLYCVILILVVAIHHSIYRRGEMLLSVSGQ 180
           GNVFLVAFGNHQSPV+T EQL EKY N+TFL+YC ILIL+VA+HH +YR+GE+L+S  GQ
Sbjct: 121 GNVFLVAFGNHQSPVFTPEQLAEKYSNVTFLVYCGILILIVAVHHFLYRKGEVLISTPGQ 180

Query: 181 DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLLLLFLS 240
           ++  YW MLLPFSYA+VSGAIGSCSVLFAKSLS LLRLAMSS YQLHSWFTYS+LLLFLS
Sbjct: 181 EISSYWKMLLPFSYAVVSGAIGSCSVLFAKSLSNLLRLAMSSSYQLHSWFTYSMLLLFLS 240

Query: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGMM 300
           TAGFWM RLNEGLSL+DAILIVPMFQIAWTFFSICTG IYF+EFQV DALRTTMFILGMM
Sbjct: 241 TAGFWMTRLNEGLSLYDAILIVPMFQIAWTFFSICTGCIYFQEFQVFDALRTTMFILGMM 300

Query: 301 SVFIGISLLAPDEPRDGEFKDN--SLVSGTSTSFSAEEERLI-VSSKELETRDARSFSQG 360
            VFIGISLLAPD+ R  E KDN  SL S  S+S   EE+RLI  SS++  ++D R   QG
Sbjct: 301 CVFIGISLLAPDDTRGNETKDNSSSLDSIVSSSVPTEEDRLIPQSSEDGHSKDTRVVVQG 360

Query: 361 FFLKITDVAAKAKAALALSLGFGEDSLSASAVLVMPMMSSRMTGFRGTGFDRSKFFSMRK 420
            ++K  D+ AK K A   +LGFGEDS++ASA+LVMPM+SS++TGFRG G +R+K  SMR 
Sbjct: 361 MYMKAADLIAKTKTACLAALGFGEDSINASAILVMPMVSSKITGFRGNGLERAKILSMRG 420

Query: 421 PDWSKISLDDEDPKVVVNT 437
             WSK+++++E  +++  T
Sbjct: 421 SGWSKLAMEEEGTRMLEKT 436

BLAST of Csor.00g262580 vs. ExPASy Swiss-Prot
Match: Q9LIR9 (Probable magnesium transporter NIPA1 OS=Arabidopsis thaliana OX=3702 GN=At3g23870 PE=2 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 1.9e-11
Identity = 58/254 (22.83%), Postives = 122/254 (48.03%), Query Frame = 0

Query: 58  WRVGITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAF 117
           W  G+   I+G   NF ++ +A   L+  LG++  + +   A+F+L + + +  ++    
Sbjct: 56  WWAGMITMIVGEVANFAAYAFAPAILVTPLGALSIIFSAVLAHFILKEKLHMFGILGCIL 115

Query: 118 IVLGNVFLVAFGNHQSPVYTSEQLVEKYGNITFLLYCVILILVVAIHHSIY--RRGEMLL 177
            V+G+  +V    H+  + + +Q+ +      FL+Y  ++++VVAI    Y  R G+   
Sbjct: 116 CVVGSTTIVLHAPHEQKIESVKQIWQLAIEPGFLVYSAVIVIVVAILIFYYEPRYGKT-- 175

Query: 178 SVSGQDLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLL 237
                      HM++   Y  +   +GS +V+  K+++I ++L  S   Q   + T+  +
Sbjct: 176 -----------HMIV---YVGICSLMGSLTVMSVKAVAIAIKLTFSGTNQFKYFNTWIFI 235

Query: 238 LLFLSTAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMF 297
           L+  +     +  LN+ L  F+  +I P++ + +T F+I    I FK++     L+    
Sbjct: 236 LVVATCCILQINYLNKALDTFNTAVISPVYYVMFTTFTIIASMIMFKDWASQSGLKIATE 293

Query: 298 ILGMMSVFIGISLL 310
           + G +++  G  LL
Sbjct: 296 LCGFVTILSGTFLL 293

BLAST of Csor.00g262580 vs. ExPASy Swiss-Prot
Match: Q9LNK7 (Probable magnesium transporter NIPA3 OS=Arabidopsis thaliana OX=3702 GN=At1g34470 PE=2 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 2.3e-09
Identity = 63/275 (22.91%), Postives = 123/275 (44.73%), Query Frame = 0

Query: 58  WRVGITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAF 117
           W VG+   I+G   NF ++ +A   L+  LG++  + + A A+ +LH+ +    ++    
Sbjct: 67  WWVGMITMIVGEIANFAAYAFAPAILVTPLGALSIIISAALAHVILHEKLHTFGLLGCVL 126

Query: 118 IVLGNVFLVAFGNHQSPVYTSEQLVEKYGNITFLLYCVILI--LVVAIHHSIYRRGEMLL 177
            V+G++ +V     +  + +  Q+        FLLY   ++   ++ I   + + G+   
Sbjct: 127 CVVGSITIVLHAPQEQEIDSVLQVWNLATEPAFLLYAAAVVGAAIILIVQFVPQYGQS-- 186

Query: 178 SVSGQDLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLL 237
                      H+++   Y  V   +GS SV+  K+L I L+L  S   QL    T+   
Sbjct: 187 -----------HVMV---YIGVCSLVGSLSVMSVKALGIALKLTFSGMNQLIYPQTWVFT 246

Query: 238 LLFLSTAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMF 297
           L+ L+     M  LN+ L  F+  ++ P++ + +T  +I    I FK++   D  +    
Sbjct: 247 LIVLTCVITQMNYLNKALDTFNTAVVSPIYYVMFTSLTILASVIMFKDWDRQDGTQIVTE 306

Query: 298 ILGMMSVFIGISLLAPDEPRDGEFKDNSLVSGTST 331
           + G +++  G  LL          K   +V G+S+
Sbjct: 307 LCGFVTILSGTFLL---------HKTKDMVDGSSS 316

BLAST of Csor.00g262580 vs. ExPASy Swiss-Prot
Match: B3LFA3 (Probable magnesium transporter NIPA2 OS=Arabidopsis thaliana OX=3702 GN=At4g13800 PE=2 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 1.5e-08
Identity = 63/293 (21.50%), Postives = 133/293 (45.39%), Query Frame = 0

Query: 53  IYFQAWRVGITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVM 112
           +Y   W  G+   I+G   NF ++ +A   L+  LG++  + +   A+F+L + + +  +
Sbjct: 51  LYEPWWWAGMITMIVGEIANFAAYAFAPAILVTPLGALSIIFSAVLAHFILEEKLHMFGI 110

Query: 113 VATAFIVLGNVFLVAFGNHQSPVYTSEQLVEKYGNITFLLYCVILILVV--AIHHSIYRR 172
           +     V+G+  +V    H+  + + +Q+        FL Y  ++++VV   I +   R 
Sbjct: 111 LGCVLCVVGSTTIVLHAPHEQGIESVKQVWHLATEPGFLAYSAVVLVVVLALIFYYEPRY 170

Query: 173 GEMLLSVSGQDLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWF 232
           G+              HM++   Y  +   +GS +V+  K+++I ++L  S   Q   + 
Sbjct: 171 GKT-------------HMIV---YVGICSLMGSLTVMSVKAVAIAIKLTFSGMNQFKYFH 230

Query: 233 TYSLLLLFLSTAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDAL 292
            +  +++        +  LN+ L  F+  +I P++ + +T F+I    I FK++     L
Sbjct: 231 AWIFIIVVTICCILQINYLNKALDNFNTAVISPVYYVMFTTFTILASMIMFKDWASQSGL 290

Query: 293 RTTMFILGMMSVFIGISLLAPDEPRDGEFKDNSLVSGTSTSFSAEEERLIVSS 344
           +    + G +++  G  LL   + +D     NS     STS S  +  + ++S
Sbjct: 291 QIATELCGFVTILSGTFLL--HKTKD---MGNSTSLRGSTSHSPRDTPVFINS 322

BLAST of Csor.00g262580 vs. ExPASy Swiss-Prot
Match: Q94AH3 (Probable magnesium transporter NIPA4 OS=Arabidopsis thaliana OX=3702 GN=At1g71900 PE=2 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 2.0e-08
Identity = 57/259 (22.01%), Postives = 115/259 (44.40%), Query Frame = 0

Query: 53  IYFQAWRVGITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVM 112
           +Y   W +G+T  +LG   NF ++ +A   L+  LG+V  + +   A+ +L + + +  +
Sbjct: 62  LYEPLWWIGMTTMLLGEIANFAAYAFAPAILVTPLGAVSIIISAVLAHIILREKLHIFGI 121

Query: 113 VATAFIVLGNVFLVAFGNHQSPVYTSEQLVEKYGNITFLLYCVILI--LVVAIHHSIYRR 172
           +  A  V+G+  +V     +  + +  ++        F+ Y  ++I   V  I   + + 
Sbjct: 122 LGCALCVVGSTTIVLHAPQEREIDSVIEVWNLATEPAFMFYASLVIGAAVFLIIRFVPQY 181

Query: 173 GEMLLSVSGQDLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWF 232
           G+  + V                Y  +   +GS SV+  K+L I L+L  S   QL    
Sbjct: 182 GQTNVMV----------------YIGICSLVGSLSVMSVKALGIALKLTFSGTNQLFYPQ 241

Query: 233 TYSLLLLFLSTAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDAL 292
           T+   L+ L+     +  LN+ L  F+  ++ P++ + +T  +I    I FK++   +  
Sbjct: 242 TWIFTLVVLTCVVTQLNYLNKALDTFNTAIVSPIYYVMFTSLTILASVIMFKDWDRQNGT 301

Query: 293 RTTMFILGMMSVFIGISLL 310
           +    I G +++  G  LL
Sbjct: 302 QIVTEICGFVTILSGTFLL 304

BLAST of Csor.00g262580 vs. NCBI nr
Match: KAG6588852.1 (putative magnesium transporter NIPA8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 849 bits (2194), Expect = 3.94e-310
Identity = 444/444 (100.00%), Postives = 444/444 (100.00%), Query Frame = 0

Query: 1   MGEWVIGAFINLFGSLAINFGTNLLKLGHNEREKHSILENNGSNGKAPMKPIIYFQAWRV 60
           MGEWVIGAFINLFGSLAINFGTNLLKLGHNEREKHSILENNGSNGKAPMKPIIYFQAWRV
Sbjct: 1   MGEWVIGAFINLFGSLAINFGTNLLKLGHNEREKHSILENNGSNGKAPMKPIIYFQAWRV 60

Query: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120
           GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL
Sbjct: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120

Query: 121 GNVFLVAFGNHQSPVYTSEQLVEKYGNITFLLYCVILILVVAIHHSIYRRGEMLLSVSGQ 180
           GNVFLVAFGNHQSPVYTSEQLVEKYGNITFLLYCVILILVVAIHHSIYRRGEMLLSVSGQ
Sbjct: 121 GNVFLVAFGNHQSPVYTSEQLVEKYGNITFLLYCVILILVVAIHHSIYRRGEMLLSVSGQ 180

Query: 181 DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLLLLFLS 240
           DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLLLLFLS
Sbjct: 181 DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLLLLFLS 240

Query: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGMM 300
           TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGMM
Sbjct: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGMM 300

Query: 301 SVFIGISLLAPDEPRDGEFKDNSLVSGTSTSFSAEEERLIVSSKELETRDARSFSQGFFL 360
           SVFIGISLLAPDEPRDGEFKDNSLVSGTSTSFSAEEERLIVSSKELETRDARSFSQGFFL
Sbjct: 301 SVFIGISLLAPDEPRDGEFKDNSLVSGTSTSFSAEEERLIVSSKELETRDARSFSQGFFL 360

Query: 361 KITDVAAKAKAALALSLGFGEDSLSASAVLVMPMMSSRMTGFRGTGFDRSKFFSMRKPDW 420
           KITDVAAKAKAALALSLGFGEDSLSASAVLVMPMMSSRMTGFRGTGFDRSKFFSMRKPDW
Sbjct: 361 KITDVAAKAKAALALSLGFGEDSLSASAVLVMPMMSSRMTGFRGTGFDRSKFFSMRKPDW 420

Query: 421 SKISLDDEDPKVVVNTNIGFSQNL 444
           SKISLDDEDPKVVVNTNIGFSQNL
Sbjct: 421 SKISLDDEDPKVVVNTNIGFSQNL 444

BLAST of Csor.00g262580 vs. NCBI nr
Match: XP_022928410.1 (probable magnesium transporter NIPA8 isoform X1 [Cucurbita moschata])

HSP 1 Score: 847 bits (2188), Expect = 3.23e-309
Identity = 443/444 (99.77%), Postives = 443/444 (99.77%), Query Frame = 0

Query: 1   MGEWVIGAFINLFGSLAINFGTNLLKLGHNEREKHSILENNGSNGKAPMKPIIYFQAWRV 60
           MGEWVIGAFINLFGSLAINFGTNLLKLGHNEREKHSILENNGSNGKAPMKPIIYFQAWRV
Sbjct: 1   MGEWVIGAFINLFGSLAINFGTNLLKLGHNEREKHSILENNGSNGKAPMKPIIYFQAWRV 60

Query: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120
           GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL
Sbjct: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120

Query: 121 GNVFLVAFGNHQSPVYTSEQLVEKYGNITFLLYCVILILVVAIHHSIYRRGEMLLSVSGQ 180
           GNVFLVAFGNHQSPVYTSEQLVEKYGNITFLLYCVILILVVAIHHSIYRRGEMLLSVSGQ
Sbjct: 121 GNVFLVAFGNHQSPVYTSEQLVEKYGNITFLLYCVILILVVAIHHSIYRRGEMLLSVSGQ 180

Query: 181 DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLLLLFLS 240
           DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLLLLFLS
Sbjct: 181 DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLLLLFLS 240

Query: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGMM 300
           TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGMM
Sbjct: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGMM 300

Query: 301 SVFIGISLLAPDEPRDGEFKDNSLVSGTSTSFSAEEERLIVSSKELETRDARSFSQGFFL 360
           SVFIGISLLAPDEPRDGEFKDNSLVSGTSTSFSAEEERLIVSSKELETRDARSFSQGF L
Sbjct: 301 SVFIGISLLAPDEPRDGEFKDNSLVSGTSTSFSAEEERLIVSSKELETRDARSFSQGFLL 360

Query: 361 KITDVAAKAKAALALSLGFGEDSLSASAVLVMPMMSSRMTGFRGTGFDRSKFFSMRKPDW 420
           KITDVAAKAKAALALSLGFGEDSLSASAVLVMPMMSSRMTGFRGTGFDRSKFFSMRKPDW
Sbjct: 361 KITDVAAKAKAALALSLGFGEDSLSASAVLVMPMMSSRMTGFRGTGFDRSKFFSMRKPDW 420

Query: 421 SKISLDDEDPKVVVNTNIGFSQNL 444
           SKISLDDEDPKVVVNTNIGFSQNL
Sbjct: 421 SKISLDDEDPKVVVNTNIGFSQNL 444

BLAST of Csor.00g262580 vs. NCBI nr
Match: XP_022989646.1 (probable magnesium transporter NIPA8 isoform X1 [Cucurbita maxima])

HSP 1 Score: 847 bits (2187), Expect = 4.59e-309
Identity = 442/444 (99.55%), Postives = 443/444 (99.77%), Query Frame = 0

Query: 1   MGEWVIGAFINLFGSLAINFGTNLLKLGHNEREKHSILENNGSNGKAPMKPIIYFQAWRV 60
           MGEWVIGAFINLFGSLAINFGTNLLKLGHNEREKHS+LENNGSNGKAPMKPIIYFQAWRV
Sbjct: 1   MGEWVIGAFINLFGSLAINFGTNLLKLGHNEREKHSVLENNGSNGKAPMKPIIYFQAWRV 60

Query: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120
           GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL
Sbjct: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120

Query: 121 GNVFLVAFGNHQSPVYTSEQLVEKYGNITFLLYCVILILVVAIHHSIYRRGEMLLSVSGQ 180
           GNVFLVAFGNHQSPVYTSEQLVEKYGNITFLLYCVILILVVAIHHSIYRRGEMLLSVSGQ
Sbjct: 121 GNVFLVAFGNHQSPVYTSEQLVEKYGNITFLLYCVILILVVAIHHSIYRRGEMLLSVSGQ 180

Query: 181 DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLLLLFLS 240
           DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLLLLFLS
Sbjct: 181 DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLLLLFLS 240

Query: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGMM 300
           TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGMM
Sbjct: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGMM 300

Query: 301 SVFIGISLLAPDEPRDGEFKDNSLVSGTSTSFSAEEERLIVSSKELETRDARSFSQGFFL 360
           SVFIGISLLAPDEPRDGEFKDNSLVSGTSTSFSAEEERLIVSSKELETRDARSFSQGF L
Sbjct: 301 SVFIGISLLAPDEPRDGEFKDNSLVSGTSTSFSAEEERLIVSSKELETRDARSFSQGFLL 360

Query: 361 KITDVAAKAKAALALSLGFGEDSLSASAVLVMPMMSSRMTGFRGTGFDRSKFFSMRKPDW 420
           KITDVAAKAKAALALSLGFGEDSLSASAVLVMPMMSSRMTGFRGTGFDRSKFFSMRKPDW
Sbjct: 361 KITDVAAKAKAALALSLGFGEDSLSASAVLVMPMMSSRMTGFRGTGFDRSKFFSMRKPDW 420

Query: 421 SKISLDDEDPKVVVNTNIGFSQNL 444
           SKISLDDEDPKVVVNTNIGFSQNL
Sbjct: 421 SKISLDDEDPKVVVNTNIGFSQNL 444

BLAST of Csor.00g262580 vs. NCBI nr
Match: XP_023531588.1 (probable magnesium transporter NIPA8 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 840 bits (2171), Expect = 1.26e-306
Identity = 439/444 (98.87%), Postives = 441/444 (99.32%), Query Frame = 0

Query: 1   MGEWVIGAFINLFGSLAINFGTNLLKLGHNEREKHSILENNGSNGKAPMKPIIYFQAWRV 60
           MGEWVIGAFINLFGSLAINFGTNLLKLGHNEREKHSILENNGSNGKAPMKPIIYFQAWRV
Sbjct: 1   MGEWVIGAFINLFGSLAINFGTNLLKLGHNEREKHSILENNGSNGKAPMKPIIYFQAWRV 60

Query: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120
           GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL
Sbjct: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120

Query: 121 GNVFLVAFGNHQSPVYTSEQLVEKYGNITFLLYCVILILVVAIHHSIYRRGEMLLSVSGQ 180
           GNVFLVAFGNHQSPVYTSEQLVEKYGNITFLLYCVILILVV IHHSIYRRGE LLSVSGQ
Sbjct: 121 GNVFLVAFGNHQSPVYTSEQLVEKYGNITFLLYCVILILVVVIHHSIYRRGEQLLSVSGQ 180

Query: 181 DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLLLLFLS 240
           +LRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLLLLFLS
Sbjct: 181 ELRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLLLLFLS 240

Query: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGMM 300
           TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGMM
Sbjct: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGMM 300

Query: 301 SVFIGISLLAPDEPRDGEFKDNSLVSGTSTSFSAEEERLIVSSKELETRDARSFSQGFFL 360
           SVFIGISLLAPDEPRDGEFKDNSLVSGTSTSFSAEEERLIVSSKELETR+ARSFSQGF L
Sbjct: 301 SVFIGISLLAPDEPRDGEFKDNSLVSGTSTSFSAEEERLIVSSKELETREARSFSQGFLL 360

Query: 361 KITDVAAKAKAALALSLGFGEDSLSASAVLVMPMMSSRMTGFRGTGFDRSKFFSMRKPDW 420
           KITDVAAKAKAALALSLGFGEDSLSASAVLVMPMMSSRMTGFRGTGFDRSKFFSMRKPDW
Sbjct: 361 KITDVAAKAKAALALSLGFGEDSLSASAVLVMPMMSSRMTGFRGTGFDRSKFFSMRKPDW 420

Query: 421 SKISLDDEDPKVVVNTNIGFSQNL 444
           SKISLDDEDPKVVVNTNIGFSQNL
Sbjct: 421 SKISLDDEDPKVVVNTNIGFSQNL 444

BLAST of Csor.00g262580 vs. NCBI nr
Match: XP_004145621.2 (probable magnesium transporter NIPA8 [Cucumis sativus] >XP_031738794.1 probable magnesium transporter NIPA8 [Cucumis sativus] >KAE8650557.1 hypothetical protein Csa_010669 [Cucumis sativus])

HSP 1 Score: 798 bits (2061), Expect = 7.27e-290
Identity = 418/445 (93.93%), Postives = 434/445 (97.53%), Query Frame = 0

Query: 1   MGEWVIGAFINLFGSLAINFGTNLLKLGHNEREKHSILENNGSNGKAPMKPIIYFQAWRV 60
           MGEWVIGA INLFGSLAINFGTNLLKLGHNEREKHS+LENNGS GK PMKPIIYFQ WRV
Sbjct: 1   MGEWVIGAVINLFGSLAINFGTNLLKLGHNEREKHSMLENNGSIGKTPMKPIIYFQTWRV 60

Query: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120
           GITFFI+GNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL
Sbjct: 61  GITFFIIGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120

Query: 121 GNVFLVAFGNHQSPVYTSEQLVEKYGNITFLLYCVILILVVAIHHSIYRRGEMLLSVSGQ 180
           GNVFLVAFGNHQSPVYT EQLVEKYGNITFLLYCVILILVVA+HHSIYRRGE+LLSVSGQ
Sbjct: 121 GNVFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILVVALHHSIYRRGELLLSVSGQ 180

Query: 181 DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLLLLFLS 240
           DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLLLLFLS
Sbjct: 181 DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLLLLFLS 240

Query: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGMM 300
           TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGMM
Sbjct: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGMM 300

Query: 301 SVFIGISLLAPDEPRDGEFKDNS-LVSGTSTSFSAEEERLIVSSKELETRDARSFSQGFF 360
           SVFIGISLLAPDEPRDGEFKDNS LVSGTSTSF++EEERLIVSSKELETRDA+SFSQG  
Sbjct: 301 SVFIGISLLAPDEPRDGEFKDNSSLVSGTSTSFTSEEERLIVSSKELETRDAKSFSQGVL 360

Query: 361 LKITDVAAKAKAALALSLGFGEDSLSASAVLVMPMMSSRMTGFRGTGFDRSKFFSMRKPD 420
           LKITDV AKAKAALALSLGFGEDSL+ASA+LVMPM+SSRMTGFRGTGF+RSKFFSMRKPD
Sbjct: 361 LKITDVVAKAKAALALSLGFGEDSLNASAILVMPMVSSRMTGFRGTGFERSKFFSMRKPD 420

Query: 421 WSKISLDDEDPKVVVNTNIGFSQNL 444
           WS+ISLD+ED KV+ +T+IGFSQNL
Sbjct: 421 WSRISLDEEDTKVL-DTDIGFSQNL 444

BLAST of Csor.00g262580 vs. ExPASy TrEMBL
Match: A0A6J1ERL5 (Probable magnesium transporter OS=Cucurbita moschata OX=3662 GN=LOC111435232 PE=3 SV=1)

HSP 1 Score: 847 bits (2188), Expect = 1.56e-309
Identity = 443/444 (99.77%), Postives = 443/444 (99.77%), Query Frame = 0

Query: 1   MGEWVIGAFINLFGSLAINFGTNLLKLGHNEREKHSILENNGSNGKAPMKPIIYFQAWRV 60
           MGEWVIGAFINLFGSLAINFGTNLLKLGHNEREKHSILENNGSNGKAPMKPIIYFQAWRV
Sbjct: 1   MGEWVIGAFINLFGSLAINFGTNLLKLGHNEREKHSILENNGSNGKAPMKPIIYFQAWRV 60

Query: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120
           GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL
Sbjct: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120

Query: 121 GNVFLVAFGNHQSPVYTSEQLVEKYGNITFLLYCVILILVVAIHHSIYRRGEMLLSVSGQ 180
           GNVFLVAFGNHQSPVYTSEQLVEKYGNITFLLYCVILILVVAIHHSIYRRGEMLLSVSGQ
Sbjct: 121 GNVFLVAFGNHQSPVYTSEQLVEKYGNITFLLYCVILILVVAIHHSIYRRGEMLLSVSGQ 180

Query: 181 DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLLLLFLS 240
           DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLLLLFLS
Sbjct: 181 DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLLLLFLS 240

Query: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGMM 300
           TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGMM
Sbjct: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGMM 300

Query: 301 SVFIGISLLAPDEPRDGEFKDNSLVSGTSTSFSAEEERLIVSSKELETRDARSFSQGFFL 360
           SVFIGISLLAPDEPRDGEFKDNSLVSGTSTSFSAEEERLIVSSKELETRDARSFSQGF L
Sbjct: 301 SVFIGISLLAPDEPRDGEFKDNSLVSGTSTSFSAEEERLIVSSKELETRDARSFSQGFLL 360

Query: 361 KITDVAAKAKAALALSLGFGEDSLSASAVLVMPMMSSRMTGFRGTGFDRSKFFSMRKPDW 420
           KITDVAAKAKAALALSLGFGEDSLSASAVLVMPMMSSRMTGFRGTGFDRSKFFSMRKPDW
Sbjct: 361 KITDVAAKAKAALALSLGFGEDSLSASAVLVMPMMSSRMTGFRGTGFDRSKFFSMRKPDW 420

Query: 421 SKISLDDEDPKVVVNTNIGFSQNL 444
           SKISLDDEDPKVVVNTNIGFSQNL
Sbjct: 421 SKISLDDEDPKVVVNTNIGFSQNL 444

BLAST of Csor.00g262580 vs. ExPASy TrEMBL
Match: A0A6J1JKP0 (Probable magnesium transporter OS=Cucurbita maxima OX=3661 GN=LOC111486667 PE=3 SV=1)

HSP 1 Score: 847 bits (2187), Expect = 2.22e-309
Identity = 442/444 (99.55%), Postives = 443/444 (99.77%), Query Frame = 0

Query: 1   MGEWVIGAFINLFGSLAINFGTNLLKLGHNEREKHSILENNGSNGKAPMKPIIYFQAWRV 60
           MGEWVIGAFINLFGSLAINFGTNLLKLGHNEREKHS+LENNGSNGKAPMKPIIYFQAWRV
Sbjct: 1   MGEWVIGAFINLFGSLAINFGTNLLKLGHNEREKHSVLENNGSNGKAPMKPIIYFQAWRV 60

Query: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120
           GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL
Sbjct: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120

Query: 121 GNVFLVAFGNHQSPVYTSEQLVEKYGNITFLLYCVILILVVAIHHSIYRRGEMLLSVSGQ 180
           GNVFLVAFGNHQSPVYTSEQLVEKYGNITFLLYCVILILVVAIHHSIYRRGEMLLSVSGQ
Sbjct: 121 GNVFLVAFGNHQSPVYTSEQLVEKYGNITFLLYCVILILVVAIHHSIYRRGEMLLSVSGQ 180

Query: 181 DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLLLLFLS 240
           DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLLLLFLS
Sbjct: 181 DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLLLLFLS 240

Query: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGMM 300
           TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGMM
Sbjct: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGMM 300

Query: 301 SVFIGISLLAPDEPRDGEFKDNSLVSGTSTSFSAEEERLIVSSKELETRDARSFSQGFFL 360
           SVFIGISLLAPDEPRDGEFKDNSLVSGTSTSFSAEEERLIVSSKELETRDARSFSQGF L
Sbjct: 301 SVFIGISLLAPDEPRDGEFKDNSLVSGTSTSFSAEEERLIVSSKELETRDARSFSQGFLL 360

Query: 361 KITDVAAKAKAALALSLGFGEDSLSASAVLVMPMMSSRMTGFRGTGFDRSKFFSMRKPDW 420
           KITDVAAKAKAALALSLGFGEDSLSASAVLVMPMMSSRMTGFRGTGFDRSKFFSMRKPDW
Sbjct: 361 KITDVAAKAKAALALSLGFGEDSLSASAVLVMPMMSSRMTGFRGTGFDRSKFFSMRKPDW 420

Query: 421 SKISLDDEDPKVVVNTNIGFSQNL 444
           SKISLDDEDPKVVVNTNIGFSQNL
Sbjct: 421 SKISLDDEDPKVVVNTNIGFSQNL 444

BLAST of Csor.00g262580 vs. ExPASy TrEMBL
Match: A0A1S3BN84 (Probable magnesium transporter OS=Cucumis melo OX=3656 GN=LOC103491699 PE=3 SV=1)

HSP 1 Score: 796 bits (2057), Expect = 1.43e-289
Identity = 418/445 (93.93%), Postives = 433/445 (97.30%), Query Frame = 0

Query: 1   MGEWVIGAFINLFGSLAINFGTNLLKLGHNEREKHSILENNGSNGKAPMKPIIYFQAWRV 60
           MGEWVIGA INLFGSLAINFGTNLLKLGHNEREKHS+LENNGS GK PMKPIIYFQ WRV
Sbjct: 1   MGEWVIGAVINLFGSLAINFGTNLLKLGHNEREKHSMLENNGSIGKTPMKPIIYFQTWRV 60

Query: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120
           GITFFI+GNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL
Sbjct: 61  GITFFIIGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120

Query: 121 GNVFLVAFGNHQSPVYTSEQLVEKYGNITFLLYCVILILVVAIHHSIYRRGEMLLSVSGQ 180
           GNVFLVAFGNHQSPVYT EQLVEKYGNITFLLYCVILI+VVA+HHSIYRRGE+LLSVSGQ
Sbjct: 121 GNVFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILIVVVALHHSIYRRGELLLSVSGQ 180

Query: 181 DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLLLLFLS 240
           DLR YWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLLLLFLS
Sbjct: 181 DLRSYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLLLLFLS 240

Query: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGMM 300
           TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGMM
Sbjct: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGMM 300

Query: 301 SVFIGISLLAPDEPRDGEFKDNS-LVSGTSTSFSAEEERLIVSSKELETRDARSFSQGFF 360
           SVFIGISLLAPDEPRDGEFKDNS LVSGTSTSF++EEERLIVSSKE ETRDA+SFSQGF 
Sbjct: 301 SVFIGISLLAPDEPRDGEFKDNSSLVSGTSTSFTSEEERLIVSSKESETRDAKSFSQGFL 360

Query: 361 LKITDVAAKAKAALALSLGFGEDSLSASAVLVMPMMSSRMTGFRGTGFDRSKFFSMRKPD 420
           LKITDV AKAKAALALSLGFGEDSL+ASAVLVMPM+SSRMTGFRGTGF+RSKFFSMRKPD
Sbjct: 361 LKITDVVAKAKAALALSLGFGEDSLNASAVLVMPMVSSRMTGFRGTGFERSKFFSMRKPD 420

Query: 421 WSKISLDDEDPKVVVNTNIGFSQNL 444
           WS+ISLD+ED KV+ +TNIGFSQNL
Sbjct: 421 WSRISLDEEDTKVL-DTNIGFSQNL 444

BLAST of Csor.00g262580 vs. ExPASy TrEMBL
Match: A0A6J1F408 (Probable magnesium transporter OS=Cucurbita moschata OX=3662 GN=LOC111442184 PE=3 SV=1)

HSP 1 Score: 778 bits (2009), Expect = 2.83e-282
Identity = 410/445 (92.13%), Postives = 429/445 (96.40%), Query Frame = 0

Query: 1   MGEWVIGAFINLFGSLAINFGTNLLKLGHNEREKHSILENNGSNGKAPMKPIIYFQAWRV 60
           MGEWVIGA INLFGS+AINFGTNLLKLGHNEREKHS+LENNGSNGK+PMKPIIYFQ WR+
Sbjct: 1   MGEWVIGAVINLFGSIAINFGTNLLKLGHNEREKHSMLENNGSNGKSPMKPIIYFQTWRI 60

Query: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120
           G+TFFI+GNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLH+MVTVKVMVATAFIVL
Sbjct: 61  GLTFFIIGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHEMVTVKVMVATAFIVL 120

Query: 121 GNVFLVAFGNHQSPVYTSEQLVEKYGNITFLLYCVILILVVAIHHSIYRRGEMLLSVSGQ 180
           GN FLVAFGNHQSPVYT EQLVEKYGNITFLLYCVILI VVA+HHSIYRRGE+LLSVSGQ
Sbjct: 121 GNSFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILIFVVALHHSIYRRGELLLSVSGQ 180

Query: 181 DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLLLLFLS 240
           DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLLLLFLS
Sbjct: 181 DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLLLLFLS 240

Query: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGMM 300
           TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGMM
Sbjct: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGMM 300

Query: 301 SVFIGISLLAPDEPRDGEFKDNS-LVSGTSTSFSAEEERLIVSSKELETRDARSFSQGFF 360
           SVFIGISLLAPDEPRDGEFKDNS L+S TSTSFS+EEERLIVSS+ELET+DARSFSQGF 
Sbjct: 301 SVFIGISLLAPDEPRDGEFKDNSPLLSETSTSFSSEEERLIVSSQELETKDARSFSQGFL 360

Query: 361 LKITDVAAKAKAALALSLGFGEDSLSASAVLVMPMMSSRMTGFRGTGFDRSKFFSMRKPD 420
           LKITDV AK KAALALSLGFGEDSL+ASAVLVMPM+SSRMTGFRGTG +RSK FSMRK D
Sbjct: 361 LKITDVVAKGKAALALSLGFGEDSLNASAVLVMPMVSSRMTGFRGTGLERSKPFSMRKSD 420

Query: 421 WSKISLDDEDPKVVVNTNIGFSQNL 444
           WSKISLD ED KV+ +T+IGFSQNL
Sbjct: 421 WSKISLD-EDAKVL-DTSIGFSQNL 443

BLAST of Csor.00g262580 vs. ExPASy TrEMBL
Match: A0A6J1J669 (Probable magnesium transporter OS=Cucurbita maxima OX=3661 GN=LOC111482128 PE=3 SV=1)

HSP 1 Score: 766 bits (1979), Expect = 1.05e-277
Identity = 404/445 (90.79%), Postives = 425/445 (95.51%), Query Frame = 0

Query: 1   MGEWVIGAFINLFGSLAINFGTNLLKLGHNEREKHSILENNGSNGKAPMKPIIYFQAWRV 60
           MGEWVIGA INLFGS+AINFGTNLLKLGHNEREKHS+LENNGSNGK PMKPIIYFQ WR+
Sbjct: 1   MGEWVIGAVINLFGSIAINFGTNLLKLGHNEREKHSMLENNGSNGKTPMKPIIYFQTWRI 60

Query: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120
           G+TFFI+GNCLNFISFGYAAQSLLAALGSVQFV+NIAFAYFVLH+MV VKVMVATAFIVL
Sbjct: 61  GLTFFIIGNCLNFISFGYAAQSLLAALGSVQFVANIAFAYFVLHEMVAVKVMVATAFIVL 120

Query: 121 GNVFLVAFGNHQSPVYTSEQLVEKYGNITFLLYCVILILVVAIHHSIYRRGEMLLSVSGQ 180
           GN FLVAFGNHQSPVYT EQLVEKYGNITFLLYCVILILVVA+HHSIYRRGE+LLSVS Q
Sbjct: 121 GNSFLVAFGNHQSPVYTPEQLVEKYGNITFLLYCVILILVVALHHSIYRRGELLLSVSRQ 180

Query: 181 DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLLLLFLS 240
           DLRPYW MLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLLLLFLS
Sbjct: 181 DLRPYWRMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLLLLFLS 240

Query: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGMM 300
           TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILG+M
Sbjct: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGLM 300

Query: 301 SVFIGISLLAPDEPRDGEFKDNS-LVSGTSTSFSAEEERLIVSSKELETRDARSFSQGFF 360
           SVFIGISLLAPDEPRDGEFKDNS L+S TSTSFS+EEERLIVSS+ELET+DARSFSQGF 
Sbjct: 301 SVFIGISLLAPDEPRDGEFKDNSTLLSETSTSFSSEEERLIVSSQELETKDARSFSQGFL 360

Query: 361 LKITDVAAKAKAALALSLGFGEDSLSASAVLVMPMMSSRMTGFRGTGFDRSKFFSMRKPD 420
           LKITDV AK K+ALALSLGFGEDSL+ASAVLVMPM+SSRMTGFRGTG +RSK FSMRK  
Sbjct: 361 LKITDVVAKGKSALALSLGFGEDSLNASAVLVMPMVSSRMTGFRGTGLERSKLFSMRKSG 420

Query: 421 WSKISLDDEDPKVVVNTNIGFSQNL 444
           WSKISLD ED KV+ +T+IGFSQNL
Sbjct: 421 WSKISLD-EDAKVL-DTSIGFSQNL 443

BLAST of Csor.00g262580 vs. TAIR 10
Match: AT3G26670.1 (Protein of unknown function (DUF803) )

HSP 1 Score: 606.7 bits (1563), Expect = 1.5e-173
Identity = 316/439 (71.98%), Postives = 370/439 (84.28%), Query Frame = 0

Query: 1   MGEWVIGAFINLFGSLAINFGTNLLKLGHNEREKHSILENNGSNGKAPMKPIIYFQAWRV 60
           MGEWVIGAFIN+FGS+AINFGTNLLKLGHNERE+ ++ +     GK P+KPII+ Q WRV
Sbjct: 1   MGEWVIGAFINIFGSVAINFGTNLLKLGHNERERLALQD---GGGKMPLKPIIHNQTWRV 60

Query: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120
           GI  F+LGNCLNFISFGYAAQSLLAALGS+QFVSNIAFAY VL+KMVTVKV+VATAFIVL
Sbjct: 61  GILVFLLGNCLNFISFGYAAQSLLAALGSIQFVSNIAFAYVVLNKMVTVKVLVATAFIVL 120

Query: 121 GNVFLVAFGNHQSPVYTSEQLVEKYGNITFLLYCVILILVVAIHHSIYRRGEMLLSVSGQ 180
           GNVFLVAFGNHQSPV+T EQL EKY N+TFL+YC ILIL+VA+HH +YR+GE+L+S  GQ
Sbjct: 121 GNVFLVAFGNHQSPVFTPEQLAEKYSNVTFLVYCGILILIVAVHHFLYRKGEVLISTPGQ 180

Query: 181 DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLLLLFLS 240
           ++  YW MLLPFSYA+VSGAIGSCSVLFAKSLS LLRLAMSS YQLHSWFTYS+LLLFLS
Sbjct: 181 EISSYWKMLLPFSYAVVSGAIGSCSVLFAKSLSNLLRLAMSSSYQLHSWFTYSMLLLFLS 240

Query: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGMM 300
           TAGFWM RLNEGLSL+DAILIVPMFQIAWTFFSICTG IYF+EFQV DALRTTMFILGMM
Sbjct: 241 TAGFWMTRLNEGLSLYDAILIVPMFQIAWTFFSICTGCIYFQEFQVFDALRTTMFILGMM 300

Query: 301 SVFIGISLLAPDEPRDGEFKDN--SLVSGTSTSFSAEEERLI-VSSKELETRDARSFSQG 360
            VFIGISLLAPD+ R  E KDN  SL S  S+S   EE+RLI  SS++  ++D R   QG
Sbjct: 301 CVFIGISLLAPDDTRGNETKDNSSSLDSIVSSSVPTEEDRLIPQSSEDGHSKDTRVVVQG 360

Query: 361 FFLKITDVAAKAKAALALSLGFGEDSLSASAVLVMPMMSSRMTGFRGTGFDRSKFFSMRK 420
            ++K  D+ AK K A   +LGFGEDS++ASA+LVMPM+SS++TGFRG G +R+K  SMR 
Sbjct: 361 MYMKAADLIAKTKTACLAALGFGEDSINASAILVMPMVSSKITGFRGNGLERAKILSMRG 420

Query: 421 PDWSKISLDDEDPKVVVNT 437
             WSK+++++E  +++  T
Sbjct: 421 SGWSKLAMEEEGTRMLEKT 436

BLAST of Csor.00g262580 vs. TAIR 10
Match: AT3G26670.2 (Protein of unknown function (DUF803) )

HSP 1 Score: 606.7 bits (1563), Expect = 1.5e-173
Identity = 316/439 (71.98%), Postives = 370/439 (84.28%), Query Frame = 0

Query: 1   MGEWVIGAFINLFGSLAINFGTNLLKLGHNEREKHSILENNGSNGKAPMKPIIYFQAWRV 60
           MGEWVIGAFIN+FGS+AINFGTNLLKLGHNERE+ ++ +     GK P+KPII+ Q WRV
Sbjct: 1   MGEWVIGAFINIFGSVAINFGTNLLKLGHNERERLALQD---GGGKMPLKPIIHNQTWRV 60

Query: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120
           GI  F+LGNCLNFISFGYAAQSLLAALGS+QFVSNIAFAY VL+KMVTVKV+VATAFIVL
Sbjct: 61  GILVFLLGNCLNFISFGYAAQSLLAALGSIQFVSNIAFAYVVLNKMVTVKVLVATAFIVL 120

Query: 121 GNVFLVAFGNHQSPVYTSEQLVEKYGNITFLLYCVILILVVAIHHSIYRRGEMLLSVSGQ 180
           GNVFLVAFGNHQSPV+T EQL EKY N+TFL+YC ILIL+VA+HH +YR+GE+L+S  GQ
Sbjct: 121 GNVFLVAFGNHQSPVFTPEQLAEKYSNVTFLVYCGILILIVAVHHFLYRKGEVLISTPGQ 180

Query: 181 DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLLLLFLS 240
           ++  YW MLLPFSYA+VSGAIGSCSVLFAKSLS LLRLAMSS YQLHSWFTYS+LLLFLS
Sbjct: 181 EISSYWKMLLPFSYAVVSGAIGSCSVLFAKSLSNLLRLAMSSSYQLHSWFTYSMLLLFLS 240

Query: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGMM 300
           TAGFWM RLNEGLSL+DAILIVPMFQIAWTFFSICTG IYF+EFQV DALRTTMFILGMM
Sbjct: 241 TAGFWMTRLNEGLSLYDAILIVPMFQIAWTFFSICTGCIYFQEFQVFDALRTTMFILGMM 300

Query: 301 SVFIGISLLAPDEPRDGEFKDN--SLVSGTSTSFSAEEERLI-VSSKELETRDARSFSQG 360
            VFIGISLLAPD+ R  E KDN  SL S  S+S   EE+RLI  SS++  ++D R   QG
Sbjct: 301 CVFIGISLLAPDDTRGNETKDNSSSLDSIVSSSVPTEEDRLIPQSSEDGHSKDTRVVVQG 360

Query: 361 FFLKITDVAAKAKAALALSLGFGEDSLSASAVLVMPMMSSRMTGFRGTGFDRSKFFSMRK 420
            ++K  D+ AK K A   +LGFGEDS++ASA+LVMPM+SS++TGFRG G +R+K  SMR 
Sbjct: 361 MYMKAADLIAKTKTACLAALGFGEDSINASAILVMPMVSSKITGFRGNGLERAKILSMRG 420

Query: 421 PDWSKISLDDEDPKVVVNT 437
             WSK+++++E  +++  T
Sbjct: 421 SGWSKLAMEEEGTRMLEKT 436

BLAST of Csor.00g262580 vs. TAIR 10
Match: AT3G26670.3 (Protein of unknown function (DUF803) )

HSP 1 Score: 606.7 bits (1563), Expect = 1.5e-173
Identity = 316/439 (71.98%), Postives = 370/439 (84.28%), Query Frame = 0

Query: 1   MGEWVIGAFINLFGSLAINFGTNLLKLGHNEREKHSILENNGSNGKAPMKPIIYFQAWRV 60
           MGEWVIGAFIN+FGS+AINFGTNLLKLGHNERE+ ++ +     GK P+KPII+ Q WRV
Sbjct: 1   MGEWVIGAFINIFGSVAINFGTNLLKLGHNERERLALQD---GGGKMPLKPIIHNQTWRV 60

Query: 61  GITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAFIVL 120
           GI  F+LGNCLNFISFGYAAQSLLAALGS+QFVSNIAFAY VL+KMVTVKV+VATAFIVL
Sbjct: 61  GILVFLLGNCLNFISFGYAAQSLLAALGSIQFVSNIAFAYVVLNKMVTVKVLVATAFIVL 120

Query: 121 GNVFLVAFGNHQSPVYTSEQLVEKYGNITFLLYCVILILVVAIHHSIYRRGEMLLSVSGQ 180
           GNVFLVAFGNHQSPV+T EQL EKY N+TFL+YC ILIL+VA+HH +YR+GE+L+S  GQ
Sbjct: 121 GNVFLVAFGNHQSPVFTPEQLAEKYSNVTFLVYCGILILIVAVHHFLYRKGEVLISTPGQ 180

Query: 181 DLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLLLLFLS 240
           ++  YW MLLPFSYA+VSGAIGSCSVLFAKSLS LLRLAMSS YQLHSWFTYS+LLLFLS
Sbjct: 181 EISSYWKMLLPFSYAVVSGAIGSCSVLFAKSLSNLLRLAMSSSYQLHSWFTYSMLLLFLS 240

Query: 241 TAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMFILGMM 300
           TAGFWM RLNEGLSL+DAILIVPMFQIAWTFFSICTG IYF+EFQV DALRTTMFILGMM
Sbjct: 241 TAGFWMTRLNEGLSLYDAILIVPMFQIAWTFFSICTGCIYFQEFQVFDALRTTMFILGMM 300

Query: 301 SVFIGISLLAPDEPRDGEFKDN--SLVSGTSTSFSAEEERLI-VSSKELETRDARSFSQG 360
            VFIGISLLAPD+ R  E KDN  SL S  S+S   EE+RLI  SS++  ++D R   QG
Sbjct: 301 CVFIGISLLAPDDTRGNETKDNSSSLDSIVSSSVPTEEDRLIPQSSEDGHSKDTRVVVQG 360

Query: 361 FFLKITDVAAKAKAALALSLGFGEDSLSASAVLVMPMMSSRMTGFRGTGFDRSKFFSMRK 420
            ++K  D+ AK K A   +LGFGEDS++ASA+LVMPM+SS++TGFRG G +R+K  SMR 
Sbjct: 361 MYMKAADLIAKTKTACLAALGFGEDSINASAILVMPMVSSKITGFRGNGLERAKILSMRG 420

Query: 421 PDWSKISLDDEDPKVVVNT 437
             WSK+++++E  +++  T
Sbjct: 421 SGWSKLAMEEEGTRMLEKT 436

BLAST of Csor.00g262580 vs. TAIR 10
Match: AT3G23870.1 (Protein of unknown function (DUF803) )

HSP 1 Score: 72.0 bits (175), Expect = 1.3e-12
Identity = 58/254 (22.83%), Postives = 122/254 (48.03%), Query Frame = 0

Query: 58  WRVGITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAF 117
           W  G+   I+G   NF ++ +A   L+  LG++  + +   A+F+L + + +  ++    
Sbjct: 56  WWAGMITMIVGEVANFAAYAFAPAILVTPLGALSIIFSAVLAHFILKEKLHMFGILGCIL 115

Query: 118 IVLGNVFLVAFGNHQSPVYTSEQLVEKYGNITFLLYCVILILVVAIHHSIY--RRGEMLL 177
            V+G+  +V    H+  + + +Q+ +      FL+Y  ++++VVAI    Y  R G+   
Sbjct: 116 CVVGSTTIVLHAPHEQKIESVKQIWQLAIEPGFLVYSAVIVIVVAILIFYYEPRYGKT-- 175

Query: 178 SVSGQDLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLL 237
                      HM++   Y  +   +GS +V+  K+++I ++L  S   Q   + T+  +
Sbjct: 176 -----------HMIV---YVGICSLMGSLTVMSVKAVAIAIKLTFSGTNQFKYFNTWIFI 235

Query: 238 LLFLSTAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMF 297
           L+  +     +  LN+ L  F+  +I P++ + +T F+I    I FK++     L+    
Sbjct: 236 LVVATCCILQINYLNKALDTFNTAVISPVYYVMFTTFTIIASMIMFKDWASQSGLKIATE 293

Query: 298 ILGMMSVFIGISLL 310
           + G +++  G  LL
Sbjct: 296 LCGFVTILSGTFLL 293

BLAST of Csor.00g262580 vs. TAIR 10
Match: AT1G34470.1 (Protein of unknown function (DUF803) )

HSP 1 Score: 65.1 bits (157), Expect = 1.6e-10
Identity = 63/275 (22.91%), Postives = 123/275 (44.73%), Query Frame = 0

Query: 58  WRVGITFFILGNCLNFISFGYAAQSLLAALGSVQFVSNIAFAYFVLHKMVTVKVMVATAF 117
           W VG+   I+G   NF ++ +A   L+  LG++  + + A A+ +LH+ +    ++    
Sbjct: 67  WWVGMITMIVGEIANFAAYAFAPAILVTPLGALSIIISAALAHVILHEKLHTFGLLGCVL 126

Query: 118 IVLGNVFLVAFGNHQSPVYTSEQLVEKYGNITFLLYCVILI--LVVAIHHSIYRRGEMLL 177
            V+G++ +V     +  + +  Q+        FLLY   ++   ++ I   + + G+   
Sbjct: 127 CVVGSITIVLHAPQEQEIDSVLQVWNLATEPAFLLYAAAVVGAAIILIVQFVPQYGQS-- 186

Query: 178 SVSGQDLRPYWHMLLPFSYAIVSGAIGSCSVLFAKSLSILLRLAMSSDYQLHSWFTYSLL 237
                      H+++   Y  V   +GS SV+  K+L I L+L  S   QL    T+   
Sbjct: 187 -----------HVMV---YIGVCSLVGSLSVMSVKALGIALKLTFSGMNQLIYPQTWVFT 246

Query: 238 LLFLSTAGFWMARLNEGLSLFDAILIVPMFQIAWTFFSICTGFIYFKEFQVLDALRTTMF 297
           L+ L+     M  LN+ L  F+  ++ P++ + +T  +I    I FK++   D  +    
Sbjct: 247 LIVLTCVITQMNYLNKALDTFNTAVVSPIYYVMFTSLTILASVIMFKDWDRQDGTQIVTE 306

Query: 298 ILGMMSVFIGISLLAPDEPRDGEFKDNSLVSGTST 331
           + G +++  G  LL          K   +V G+S+
Sbjct: 307 LCGFVTILSGTFLL---------HKTKDMVDGSSS 316

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8RWF42.1e-17271.98Probable magnesium transporter NIPA8 OS=Arabidopsis thaliana OX=3702 GN=At3g2667... [more]
Q9LIR91.9e-1122.83Probable magnesium transporter NIPA1 OS=Arabidopsis thaliana OX=3702 GN=At3g2387... [more]
Q9LNK72.3e-0922.91Probable magnesium transporter NIPA3 OS=Arabidopsis thaliana OX=3702 GN=At1g3447... [more]
B3LFA31.5e-0821.50Probable magnesium transporter NIPA2 OS=Arabidopsis thaliana OX=3702 GN=At4g1380... [more]
Q94AH32.0e-0822.01Probable magnesium transporter NIPA4 OS=Arabidopsis thaliana OX=3702 GN=At1g7190... [more]
Match NameE-valueIdentityDescription
KAG6588852.13.94e-310100.00putative magnesium transporter NIPA8, partial [Cucurbita argyrosperma subsp. sor... [more]
XP_022928410.13.23e-30999.77probable magnesium transporter NIPA8 isoform X1 [Cucurbita moschata][more]
XP_022989646.14.59e-30999.55probable magnesium transporter NIPA8 isoform X1 [Cucurbita maxima][more]
XP_023531588.11.26e-30698.87probable magnesium transporter NIPA8 isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_004145621.27.27e-29093.93probable magnesium transporter NIPA8 [Cucumis sativus] >XP_031738794.1 probable ... [more]
Match NameE-valueIdentityDescription
A0A6J1ERL51.56e-30999.77Probable magnesium transporter OS=Cucurbita moschata OX=3662 GN=LOC111435232 PE=... [more]
A0A6J1JKP02.22e-30999.55Probable magnesium transporter OS=Cucurbita maxima OX=3661 GN=LOC111486667 PE=3 ... [more]
A0A1S3BN841.43e-28993.93Probable magnesium transporter OS=Cucumis melo OX=3656 GN=LOC103491699 PE=3 SV=1[more]
A0A6J1F4082.83e-28292.13Probable magnesium transporter OS=Cucurbita moschata OX=3662 GN=LOC111442184 PE=... [more]
A0A6J1J6691.05e-27790.79Probable magnesium transporter OS=Cucurbita maxima OX=3661 GN=LOC111482128 PE=3 ... [more]
Match NameE-valueIdentityDescription
AT3G26670.11.5e-17371.98Protein of unknown function (DUF803) [more]
AT3G26670.21.5e-17371.98Protein of unknown function (DUF803) [more]
AT3G26670.31.5e-17371.98Protein of unknown function (DUF803) [more]
AT3G23870.11.3e-1222.83Protein of unknown function (DUF803) [more]
AT1G34470.11.6e-1022.91Protein of unknown function (DUF803) [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008521Magnesium transporter NIPAPFAMPF05653Mg_trans_NIPAcoord: 6..310
e-value: 5.6E-22
score: 78.2
IPR008521Magnesium transporter NIPAPANTHERPTHR12570UNCHARACTERIZEDcoord: 1..430
NoneNo IPR availablePANTHERPTHR12570:SF75MAGNESIUM TRANSPORTER-RELATEDcoord: 1..430

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g262580.m01Csor.00g262580.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1903830 magnesium ion transmembrane transport
biological_process GO:0015693 magnesium ion transport
cellular_component GO:0005769 early endosome
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0015095 magnesium ion transmembrane transporter activity