Csor.00g259720 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g259720
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionDnaJ domain containing protein
LocationCsor_Chr17: 1800630 .. 1801682 (-)
RNA-Seq ExpressionCsor.00g259720
SyntenyCsor.00g259720
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSsinglepolypeptidestart_codonstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATGGGAATAAGGACGAAGCTTTAAGATGTATTCGCATAGCGGAAGAAGCAATTGCATCGGGGAATAAAGAGCGAGCACTTAAATTCATTAAAATTGCTTGTCGCCTTAATCGGAGTTTGGGAGTGGACGAGTTATTGTCGGCGTGCGAGGAGATCGACTTGAAATCACCTTCATCTTCCTCCGATGGGAAACGCGCGGGAAAAGTTCATAGTGTTTCTGGTTCGGCGAATCATGTTGACGGTTTGAATGGTGAACGTAATTACAGTATTGAACATGTTCAATTGATCAGGCAGATAAAAACAGCTAAGGACTATTACAAAATTCTTGGTGTGGAGAGAACTAGTTCGGCTGAAGAAATTAAGCGGGCTTATAAGAAATTGTCCTTGAAAGTTCACCCTGATAAGAACAAGGCTCCTGGTTCAGATGAAGCATTCAAGAAATTATCCAAGGCTTTCATGTGTTTGAGCGATGACACATCGAGAAGGCAATATGACCACACAGCTTTGGTTGATCAATATGAGTACAACCAACAGCATAATGTAAGGCGCAGGAGAACTGGTCGTGATTTGTTTGAAGAAAATTTTGATCCTGATGAGATATTCAGGGCGTTCTTTGGGCAAGGAAATATGTTTCAGACGAGTCGTGCTTATACTTATAGCACTGGAGGTGCGAGAAGCCAACGGAGGACAGAATCTGATGGGGGAGGTCCCAACTTGTTGATTTTACTTCTAATGTTACCATTCTTGTTAATTGTCTTGTTGGCTTATATGCCCTTTCCAGAGCCAGACTATTCTTTGCATAAAAATCTATCCTACAGTATTCCCATGGCTACGGAGAAACATGGAGTGGAGTTTTTTGTCAAATCATCAGATTTTGATGAAAGATATCCTCTTGGAAGCGGAGCTCGATCTGAAATAGAGAGTAGTGTGATTAGGGATTACAAAAACATGGTTTGGCGTTATTGTCATGTAGAACTCCAGAGACGCAAGTGGAATAAAAATCTCCCAACTCCTCATTGCGAGAAGATGAATAATCTCGGGTTTGCATAA

mRNA sequence

ATGGATGGGAATAAGGACGAAGCTTTAAGATGTATTCGCATAGCGGAAGAAGCAATTGCATCGGGGAATAAAGAGCGAGCACTTAAATTCATTAAAATTGCTTGTCGCCTTAATCGGAGTTTGGGAGTGGACGAGTTATTGTCGGCGTGCGAGGAGATCGACTTGAAATCACCTTCATCTTCCTCCGATGGGAAACGCGCGGGAAAAGTTCATAGTGTTTCTGGTTCGGCGAATCATGTTGACGGTTTGAATGGTGAACGTAATTACAGTATTGAACATGTTCAATTGATCAGGCAGATAAAAACAGCTAAGGACTATTACAAAATTCTTGGTGTGGAGAGAACTAGTTCGGCTGAAGAAATTAAGCGGGCTTATAAGAAATTGTCCTTGAAAGTTCACCCTGATAAGAACAAGGCTCCTGGTTCAGATGAAGCATTCAAGAAATTATCCAAGGCTTTCATGTGTTTGAGCGATGACACATCGAGAAGGCAATATGACCACACAGCTTTGGTTGATCAATATGAGTACAACCAACAGCATAATGTAAGGCGCAGGAGAACTGGTCGTGATTTGTTTGAAGAAAATTTTGATCCTGATGAGATATTCAGGGCGTTCTTTGGGCAAGGAAATATGTTTCAGACGAGTCGTGCTTATACTTATAGCACTGGAGGTGCGAGAAGCCAACGGAGGACAGAATCTGATGGGGGAGGTCCCAACTTGTTGATTTTACTTCTAATGTTACCATTCTTGTTAATTGTCTTGTTGGCTTATATGCCCTTTCCAGAGCCAGACTATTCTTTGCATAAAAATCTATCCTACAGTATTCCCATGGCTACGGAGAAACATGGAGTGGAGTTTTTTGTCAAATCATCAGATTTTGATGAAAGATATCCTCTTGGAAGCGGAGCTCGATCTGAAATAGAGAGTAGTGTGATTAGGGATTACAAAAACATGGTTTGGCGTTATTGTCATGTAGAACTCCAGAGACGCAAGTGGAATAAAAATCTCCCAACTCCTCATTGCGAGAAGATGAATAATCTCGGGTTTGCATAA

Coding sequence (CDS)

ATGGATGGGAATAAGGACGAAGCTTTAAGATGTATTCGCATAGCGGAAGAAGCAATTGCATCGGGGAATAAAGAGCGAGCACTTAAATTCATTAAAATTGCTTGTCGCCTTAATCGGAGTTTGGGAGTGGACGAGTTATTGTCGGCGTGCGAGGAGATCGACTTGAAATCACCTTCATCTTCCTCCGATGGGAAACGCGCGGGAAAAGTTCATAGTGTTTCTGGTTCGGCGAATCATGTTGACGGTTTGAATGGTGAACGTAATTACAGTATTGAACATGTTCAATTGATCAGGCAGATAAAAACAGCTAAGGACTATTACAAAATTCTTGGTGTGGAGAGAACTAGTTCGGCTGAAGAAATTAAGCGGGCTTATAAGAAATTGTCCTTGAAAGTTCACCCTGATAAGAACAAGGCTCCTGGTTCAGATGAAGCATTCAAGAAATTATCCAAGGCTTTCATGTGTTTGAGCGATGACACATCGAGAAGGCAATATGACCACACAGCTTTGGTTGATCAATATGAGTACAACCAACAGCATAATGTAAGGCGCAGGAGAACTGGTCGTGATTTGTTTGAAGAAAATTTTGATCCTGATGAGATATTCAGGGCGTTCTTTGGGCAAGGAAATATGTTTCAGACGAGTCGTGCTTATACTTATAGCACTGGAGGTGCGAGAAGCCAACGGAGGACAGAATCTGATGGGGGAGGTCCCAACTTGTTGATTTTACTTCTAATGTTACCATTCTTGTTAATTGTCTTGTTGGCTTATATGCCCTTTCCAGAGCCAGACTATTCTTTGCATAAAAATCTATCCTACAGTATTCCCATGGCTACGGAGAAACATGGAGTGGAGTTTTTTGTCAAATCATCAGATTTTGATGAAAGATATCCTCTTGGAAGCGGAGCTCGATCTGAAATAGAGAGTAGTGTGATTAGGGATTACAAAAACATGGTTTGGCGTTATTGTCATGTAGAACTCCAGAGACGCAAGTGGAATAAAAATCTCCCAACTCCTCATTGCGAGAAGATGAATAATCTCGGGTTTGCATAA

Protein sequence

MDGNKDEALRCIRIAEEAIASGNKERALKFIKIACRLNRSLGVDELLSACEEIDLKSPSSSSDGKRAGKVHSVSGSANHVDGLNGERNYSIEHVQLIRQIKTAKDYYKILGVERTSSAEEIKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTSRRQYDHTALVDQYEYNQQHNVRRRRTGRDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYSTGGARSQRRTESDGGGPNLLILLLMLPFLLIVLLAYMPFPEPDYSLHKNLSYSIPMATEKHGVEFFVKSSDFDERYPLGSGARSEIESSVIRDYKNMVWRYCHVELQRRKWNKNLPTPHCEKMNNLGFA
Homology
BLAST of Csor.00g259720 vs. ExPASy Swiss-Prot
Match: Q9FH28 (Chaperone protein dnaJ 49 OS=Arabidopsis thaliana OX=3702 GN=ATJ49 PE=2 SV=2)

HSP 1 Score: 348.2 bits (892), Expect = 1.1e-94
Identity = 184/355 (51.83%), Postives = 256/355 (72.11%), Query Frame = 0

Query: 1   MDGNKDEALRCIRIAEEAIASGNKERALKFIKIACRLNRSLGVDELLSACEEIDLKSPSS 60
           MDGNKD+A RC+RIAE+AI SG+KERALKFI +A RLN SL VDEL++AC+ +D  S +S
Sbjct: 1   MDGNKDDASRCLRIAEDAIVSGDKERALKFINMAKRLNPSLSVDELVAACDNLDSVSRNS 60

Query: 61  SSDGKRAGKVHSVSGSANHVDGLNGERNYSIEHVQLIRQIKTAKDYYKILGVERTSSAEE 120
           S     + K+ ++ G  + ++   G+  Y+ E+V L+R I    DYY ILG+E+  S +E
Sbjct: 61  SV----SEKLKTMDGDDDKLE--TGKMKYTEENVDLVRNIIRNNDYYAILGLEKNCSVDE 120

Query: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTSRRQYDHTALVDQYEYNQQH 180
           I++AY+KLSLKVHPDKNKAPGS+EAFKK+SKAF CLSD  SRRQ+D   +VD++++ Q+ 
Sbjct: 121 IRKAYRKLSLKVHPDKNKAPGSEEAFKKVSKAFTCLSDGNSRRQFDQVGIVDEFDHVQRR 180

Query: 181 NVRRRR---TGRDLFEENFDPDEIFRAFFG-QGNMFQTSRAYTYSTGGARSQ-RRTESDG 240
           N R RR   T  D F++ FDP+EIFR  FG Q  +F+ S A  Y T   R+Q R  E + 
Sbjct: 181 NRRPRRRYNTRNDFFDDEFDPEEIFRTVFGQQREVFRASHA--YRTRQPRNQFREEEINV 240

Query: 241 GGPNLLILLLMLPFLLIVLLAYMPFPEPDYSLHKNLSYSIPMATEKHGVEFFVKS-SDFD 300
            GP+ L ++ +LPF L++LLAY+PF EPDYSLHKN SY IP  T+   + F+V+S S FD
Sbjct: 241 AGPSCLTIIQILPFFLLLLLAYLPFSEPDYSLHKNQSYQIPKTTQNTEISFYVRSASAFD 300

Query: 301 ERYPLGSGARSEIESSVIRDYKNMVWRYCHVELQRRKWNKNLPTPHCEKMNNLGF 350
           E++PL S AR+ +E +VI++YK+ +++ C +ELQ+R+WNK +PTPHC ++ + GF
Sbjct: 301 EKFPLSSSARANLEGNVIKEYKHFLFQSCRIELQKRRWNKKIPTPHCIELQDRGF 347

BLAST of Csor.00g259720 vs. ExPASy Swiss-Prot
Match: Q28I38 (DnaJ homolog subfamily B member 14 OS=Xenopus tropicalis OX=8364 GN=dnajb14 PE=2 SV=1)

HSP 1 Score: 159.5 bits (402), Expect = 7.1e-38
Identity = 114/339 (33.63%), Postives = 172/339 (50.74%), Query Frame = 0

Query: 1   MDGNKDEALRCIRIAEEAIASGNKERALKFIKIACRLNRSLGVDELLSACEEIDL--KSP 60
           M+ N+DEA RC+RIA+ AI +G+KE+A +F+  A RL  S     LL A E+ D     P
Sbjct: 1   MESNRDEAERCVRIAKAAIEAGDKEKAKRFLSKAERLYPSSEARALLQAFEKNDTAGNGP 60

Query: 61  SSS--SDGKRAGKVHSVSGSANHVDGLNGERNYSIEHVQLIRQIKTAKDYYKILGVERTS 120
            S+  + G    K    S ++   D   G     ++ VQ I++ KT   YY++LGV   +
Sbjct: 61  QSAKMAKGTEQPKAEKDSNASASSDTGKGHTQDQLDGVQRIKKCKT---YYEVLGVSTDA 120

Query: 121 SAEEIKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTSRRQYDHTALVDQYEY 180
             E++K+AY+KL+LK HPDKN APG+ EAFKK+  A+  LS+   R+QYD T   DQ + 
Sbjct: 121 GEEDLKKAYRKLALKFHPDKNHAPGATEAFKKIGNAYAVLSNPEKRKQYDLTGSEDQMQN 180

Query: 181 NQQHNVRRRRTGRDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYSTGGAR---------- 240
           N ++       G   FE +  P+++F  FFG G  F +   +T+S G AR          
Sbjct: 181 NHRNGGFDYHRG---FEADITPEDLFNMFFGGG--FPSGSVHTFSNGRARYSHHQHHHHS 240

Query: 241 --SQRRTESDGGGPNLLILLLMLPFLLIVLLAYMPFPEPDYSLHKNLSYSIPMATEKHGV 300
              +    +DGG    + L+ ++  +L+ LL+      P YSL+     +    TE   +
Sbjct: 241 GHDREDERADGGFSMFIQLMPIIVLILVSLLSQFMVSNPPYSLYPRSGQATKRVTENLQI 300

Query: 301 EFFVKSSDFDERYP--LGSGARSEIESSVIRDYKNMVWR 322
            ++V S DF   Y   L       IE   + + +N  WR
Sbjct: 301 AYYV-SKDFQSEYSGILLQKLEKNIEEDYVANVRNNCWR 330

BLAST of Csor.00g259720 vs. ExPASy Swiss-Prot
Match: Q7ZXQ8 (DnaJ homolog subfamily B member 14 OS=Xenopus laevis OX=8355 GN=dnajb14 PE=2 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 6.0e-37
Identity = 107/337 (31.75%), Postives = 173/337 (51.34%), Query Frame = 0

Query: 1   MDGNKDEALRCIRIAEEAIASGNKERALKFIKIACRLNRSLGVDELLSACEEIDLKSPSS 60
           M+ N+DEA RC+RI + AI +G+KE+A +F   A RL  S     LL A E+ D     +
Sbjct: 1   MESNRDEAERCVRIGKAAIEAGDKEKARRFFSKAERLYPSSEARVLLDALEKND-----T 60

Query: 61  SSDGKRAGKVHSVSGSANHVDGLNGE--RNYSIEHVQLIRQIKTAKDYYKILGVERTSSA 120
           + +G ++ K+   +         +G+  + ++ + V  +++IK  K YY++LGV   +  
Sbjct: 61  AGNGPQSEKMSKSTEQPKAEKDSSGDTGKGHTQDQVDGVQRIKKCKTYYEVLGVSPDAGE 120

Query: 121 EEIKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTSRRQYDHTALVDQYEYNQ 180
           E++K+AY+KL+LK HPDKN APG+ EAFKK+  A+  LS+   R+QYD T   D  + N 
Sbjct: 121 EDLKKAYRKLALKFHPDKNHAPGATEAFKKIGNAYAVLSNPEKRKQYDLTGSEDNVQNNH 180

Query: 181 QHNVRRRRTGRDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYSTGGAR------------ 240
           ++       G   FE +  P+++F  FFG G  F +   +T+S G  R            
Sbjct: 181 RNGGFDYHRG---FEADITPEDLFNMFFGGG--FPSGSVHTFSNGRTRYSHHQHHHHSGH 240

Query: 241 SQRRTESDGGGPNLLILLLMLPFLLIVLLAYMPFPEPDYSLHKNLSYSIPMATEKHGVEF 300
            +    +DGG    + L+ ++  +L+ LL+ +    P YSL+     +I   TE   + +
Sbjct: 241 DREEERADGGFSMFIQLMPIIVLILVSLLSQLMVSNPPYSLYPRSGQTIKRVTENLQISY 300

Query: 301 FVKSSDFDERY--PLGSGARSEIESSVIRDYKNMVWR 322
           +V S DF   Y   L       IE   + + +N  WR
Sbjct: 301 YV-SKDFKSEYNGMLLQKLEKNIEEDYVANVRNNCWR 326

BLAST of Csor.00g259720 vs. ExPASy Swiss-Prot
Match: Q9QYI4 (DnaJ homolog subfamily B member 12 OS=Mus musculus OX=10090 GN=Dnajb12 PE=1 SV=2)

HSP 1 Score: 153.3 bits (386), Expect = 5.1e-36
Identity = 114/345 (33.04%), Postives = 179/345 (51.88%), Query Frame = 0

Query: 1   MDGNKDEALRCIRIAEEAIASGNKERALKFIKIACRLNRSLGVDELLSACEEIDLKSPSS 60
           M+ NKDEA RCI IA +AI S   ERAL+F++ A RL  +  V  L+   E ++ K  S+
Sbjct: 1   MESNKDEAERCISIALKAIQSNQPERALRFLEKAQRLYPTPRVSALI---ESLNQKPQST 60

Query: 61  SSDGKRAGKVHSVSGSANHVD--GLNGE-------RNYSIEHVQLIRQIKTAKDYYKILG 120
               +     H+ +  A   +    NGE       + Y+ E V  ++++K  KDYY+ILG
Sbjct: 61  GDHPQPTDTTHTTTKKAGGTETPSANGEAGGGESAKGYTSEQVAAVKRVKQCKDYYEILG 120

Query: 121 VERTSSAEEIKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTSRRQYDHTALV 180
           V R++S E++K+AY+KL+LK HPDKN APG+ EAFK +  A+  LS+   R+QY      
Sbjct: 121 VSRSASDEDLKKAYRKLALKFHPDKNHAPGATEAFKAIGTAYAVLSNPEKRKQY------ 180

Query: 181 DQYEYNQQHNVRRRRTGRDL---FEENFDPDEIFRAFFGQGNMFQTSRAYTYSTGGAR-- 240
           DQ+  ++    R   +  D    FE +  P+++F  FFG G  F +S  + YS G  R  
Sbjct: 181 DQFGDDKSQAARHGHSHGDFHRGFEADISPEDLFNMFFGGG--FPSSNVHVYSNGRMRYT 240

Query: 241 -----SQRRTESDGGGPNLLILLLMLPFLLIVLLAYMPFPEPDYSL--HKNLSYSIPMAT 300
                 +R  + DGG    + L+ +L  +L+  L+ +    P YSL    ++ +     T
Sbjct: 241 YQQRQDRRDNQGDGGLGVFVQLMPILILILVSALSQLMVSSPPYSLSPRPSVGHIHKRVT 300

Query: 301 EKHGVEFFVKSSDFDERYPLGSGARS---EIESSVIRDYKNMVWR 322
           +   V ++V +  F E Y  GS  ++    +E   I + +N  W+
Sbjct: 301 DHLNVAYYV-ADTFSEEY-TGSSLKTVERNVEDDYIANLRNNCWK 332

BLAST of Csor.00g259720 vs. ExPASy Swiss-Prot
Match: Q149L6 (DnaJ homolog subfamily B member 14 OS=Mus musculus OX=10090 GN=Dnajb14 PE=2 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 7.4e-35
Identity = 112/355 (31.55%), Postives = 183/355 (51.55%), Query Frame = 0

Query: 1   MDGNKDEALRCIRIAEEAIASGNKERALKFIKIACRLNRSLGVDELLSACEEIDLKSPSS 60
           M+GN+DEA +C++IA EA+++GN+++A +F++ A +L        LL    EI +K+ S+
Sbjct: 1   MEGNRDEAEKCVQIAREALSAGNRDKAQRFLQKAEKLYPLPAARALL----EIIMKNGST 60

Query: 61  SSDGKRAGKVHSVSGSANHVDGLNGE-------RNYSIEHVQLIRQIKTAKDYYKILGVE 120
           +       K    S  +    G +G        + Y+ + V+ +  I   K+YY++LGV 
Sbjct: 61  AGSSTHCRKPPGSSDQSKPSCGKDGTSGAGEGGKVYTKDQVEGVLSINKCKNYYEVLGVT 120

Query: 121 RTSSAEEIKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTSRRQYDHTALVDQ 180
           + +  E++K+AY+KL+LK HPDKN APG+ +AFKK+  A+  LS+   R+QYD T   +Q
Sbjct: 121 KDAGDEDLKKAYRKLALKFHPDKNHAPGATDAFKKIGNAYAVLSNPEKRKQYDLTGSEEQ 180

Query: 181 YEYNQQHNVRRRRTGRDLF----EENFDPDEIFRAFFGQGNMFQTSRAYTYSTGGA---- 240
              N Q+N      GR  F    E +  P+++F  FFG G  F +   +++S G A    
Sbjct: 181 -ACNHQNN------GRFNFHRGCEADITPEDLFNIFFGGG--FPSGSVHSFSNGRAAYSH 240

Query: 241 --------RSQRRTESDGGGPNLLILLLMLPFLLIVLLAYMPFPEPDYSLH--KNLSYSI 300
                     +    +DGG    + L+ ++  +L+ LL+ +    P YSL+       +I
Sbjct: 241 QHQHRHSGHEREEERADGGFSVFIQLMPIIVLILVSLLSQLMVSNPPYSLYPRSGSGQTI 300

Query: 301 PMATEKHGVEFFVKSSDFDERYPLGSGARSEIESSVIRDYKNMVWRYCHVELQRR 331
            M TE  GV ++V S DF   Y        ++E SV  DY   +   C  E Q++
Sbjct: 301 KMQTENLGVVYYV-SKDFKSEYK--GTLLQKVEKSVEEDYVTNIRNNCWKERQQK 339

BLAST of Csor.00g259720 vs. NCBI nr
Match: KAG6575070.1 (Chaperone protein dnaJ 49, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 701 bits (1809), Expect = 1.22e-254
Identity = 350/350 (100.00%), Postives = 350/350 (100.00%), Query Frame = 0

Query: 1   MDGNKDEALRCIRIAEEAIASGNKERALKFIKIACRLNRSLGVDELLSACEEIDLKSPSS 60
           MDGNKDEALRCIRIAEEAIASGNKERALKFIKIACRLNRSLGVDELLSACEEIDLKSPSS
Sbjct: 1   MDGNKDEALRCIRIAEEAIASGNKERALKFIKIACRLNRSLGVDELLSACEEIDLKSPSS 60

Query: 61  SSDGKRAGKVHSVSGSANHVDGLNGERNYSIEHVQLIRQIKTAKDYYKILGVERTSSAEE 120
           SSDGKRAGKVHSVSGSANHVDGLNGERNYSIEHVQLIRQIKTAKDYYKILGVERTSSAEE
Sbjct: 61  SSDGKRAGKVHSVSGSANHVDGLNGERNYSIEHVQLIRQIKTAKDYYKILGVERTSSAEE 120

Query: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTSRRQYDHTALVDQYEYNQQH 180
           IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTSRRQYDHTALVDQYEYNQQH
Sbjct: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTSRRQYDHTALVDQYEYNQQH 180

Query: 181 NVRRRRTGRDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYSTGGARSQRRTESDGGGPNL 240
           NVRRRRTGRDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYSTGGARSQRRTESDGGGPNL
Sbjct: 181 NVRRRRTGRDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYSTGGARSQRRTESDGGGPNL 240

Query: 241 LILLLMLPFLLIVLLAYMPFPEPDYSLHKNLSYSIPMATEKHGVEFFVKSSDFDERYPLG 300
           LILLLMLPFLLIVLLAYMPFPEPDYSLHKNLSYSIPMATEKHGVEFFVKSSDFDERYPLG
Sbjct: 241 LILLLMLPFLLIVLLAYMPFPEPDYSLHKNLSYSIPMATEKHGVEFFVKSSDFDERYPLG 300

Query: 301 SGARSEIESSVIRDYKNMVWRYCHVELQRRKWNKNLPTPHCEKMNNLGFA 350
           SGARSEIESSVIRDYKNMVWRYCHVELQRRKWNKNLPTPHCEKMNNLGFA
Sbjct: 301 SGARSEIESSVIRDYKNMVWRYCHVELQRRKWNKNLPTPHCEKMNNLGFA 350

BLAST of Csor.00g259720 vs. NCBI nr
Match: KAG7013644.1 (Chaperone protein dnaJ 49 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 699 bits (1804), Expect = 7.06e-254
Identity = 349/350 (99.71%), Postives = 350/350 (100.00%), Query Frame = 0

Query: 1   MDGNKDEALRCIRIAEEAIASGNKERALKFIKIACRLNRSLGVDELLSACEEIDLKSPSS 60
           MDGNKDEALRCIRIAEEAIASGNKERALKFIKIACRLNRSLGVDELLSACEEIDLKSPSS
Sbjct: 1   MDGNKDEALRCIRIAEEAIASGNKERALKFIKIACRLNRSLGVDELLSACEEIDLKSPSS 60

Query: 61  SSDGKRAGKVHSVSGSANHVDGLNGERNYSIEHVQLIRQIKTAKDYYKILGVERTSSAEE 120
           SSDGKRAGKVHSVSGSANHVDGLNGERNYSIEHVQLIRQIKTAKDYYKILGVERTSSAEE
Sbjct: 61  SSDGKRAGKVHSVSGSANHVDGLNGERNYSIEHVQLIRQIKTAKDYYKILGVERTSSAEE 120

Query: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTSRRQYDHTALVDQYEYNQQH 180
           IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTSRRQYDHTALVDQYEYNQQH
Sbjct: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTSRRQYDHTALVDQYEYNQQH 180

Query: 181 NVRRRRTGRDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYSTGGARSQRRTESDGGGPNL 240
           NVRRRRTGRDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYSTGGARSQRRTESDGGGPNL
Sbjct: 181 NVRRRRTGRDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYSTGGARSQRRTESDGGGPNL 240

Query: 241 LILLLMLPFLLIVLLAYMPFPEPDYSLHKNLSYSIPMATEKHGVEFFVKSSDFDERYPLG 300
           LILLLMLPFLLIVLLAYMPFPEPDYSLHKNLSYSIPMATEKHGVEFFVKSSDF+ERYPLG
Sbjct: 241 LILLLMLPFLLIVLLAYMPFPEPDYSLHKNLSYSIPMATEKHGVEFFVKSSDFNERYPLG 300

Query: 301 SGARSEIESSVIRDYKNMVWRYCHVELQRRKWNKNLPTPHCEKMNNLGFA 350
           SGARSEIESSVIRDYKNMVWRYCHVELQRRKWNKNLPTPHCEKMNNLGFA
Sbjct: 301 SGARSEIESSVIRDYKNMVWRYCHVELQRRKWNKNLPTPHCEKMNNLGFA 350

BLAST of Csor.00g259720 vs. NCBI nr
Match: XP_022958924.1 (chaperone protein dnaJ 49 [Cucurbita moschata] >XP_022958925.1 chaperone protein dnaJ 49 [Cucurbita moschata])

HSP 1 Score: 689 bits (1778), Expect = 6.49e-250
Identity = 343/350 (98.00%), Postives = 348/350 (99.43%), Query Frame = 0

Query: 1   MDGNKDEALRCIRIAEEAIASGNKERALKFIKIACRLNRSLGVDELLSACEEIDLKSPSS 60
           MDGNKDEALRCIRIAEEAIASGNKERALKFIKIACRLNRSL V+ELLSACE+ID KSPSS
Sbjct: 1   MDGNKDEALRCIRIAEEAIASGNKERALKFIKIACRLNRSLEVNELLSACEDIDSKSPSS 60

Query: 61  SSDGKRAGKVHSVSGSANHVDGLNGERNYSIEHVQLIRQIKTAKDYYKILGVERTSSAEE 120
           SSDGKRAGKVHSVSGSANHVDGLNGERNYSIEHVQLIRQIKTAKDYYKILGVE+TSSAEE
Sbjct: 61  SSDGKRAGKVHSVSGSANHVDGLNGERNYSIEHVQLIRQIKTAKDYYKILGVEKTSSAEE 120

Query: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTSRRQYDHTALVDQYEYNQQH 180
           IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLS+DTSRRQYDHTALVDQYEYNQQH
Sbjct: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSNDTSRRQYDHTALVDQYEYNQQH 180

Query: 181 NVRRRRTGRDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYSTGGARSQRRTESDGGGPNL 240
           NVRRRRTGRDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYSTGGARSQRRTESDGGGPNL
Sbjct: 181 NVRRRRTGRDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYSTGGARSQRRTESDGGGPNL 240

Query: 241 LILLLMLPFLLIVLLAYMPFPEPDYSLHKNLSYSIPMATEKHGVEFFVKSSDFDERYPLG 300
           LILLLMLPFLLIVLLAYMPFPEPDYSLHKNLSYSIPMATEKHGVEFFVKSSDFDERYPLG
Sbjct: 241 LILLLMLPFLLIVLLAYMPFPEPDYSLHKNLSYSIPMATEKHGVEFFVKSSDFDERYPLG 300

Query: 301 SGARSEIESSVIRDYKNMVWRYCHVELQRRKWNKNLPTPHCEKMNNLGFA 350
           SGARSEIESSVIRDYKNMVWRYCH+ELQRRKWNKNLPTPHCEKMNNLGFA
Sbjct: 301 SGARSEIESSVIRDYKNMVWRYCHIELQRRKWNKNLPTPHCEKMNNLGFA 350

BLAST of Csor.00g259720 vs. NCBI nr
Match: XP_023547565.1 (chaperone protein dnaJ 49 [Cucurbita pepo subsp. pepo] >XP_023547566.1 chaperone protein dnaJ 49 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 688 bits (1775), Expect = 1.86e-249
Identity = 343/350 (98.00%), Postives = 347/350 (99.14%), Query Frame = 0

Query: 1   MDGNKDEALRCIRIAEEAIASGNKERALKFIKIACRLNRSLGVDELLSACEEIDLKSPSS 60
           MDGNKDEALRCIRIAEEAIASG+KERALKFIKIACRLNRSL V+ELLSACE+ID KSPSS
Sbjct: 1   MDGNKDEALRCIRIAEEAIASGSKERALKFIKIACRLNRSLEVNELLSACEDIDSKSPSS 60

Query: 61  SSDGKRAGKVHSVSGSANHVDGLNGERNYSIEHVQLIRQIKTAKDYYKILGVERTSSAEE 120
           SSDGKRAGKVHSVSGSANHVDGLNGERNYSIEHVQLIRQIKTAKDYYKILGVE+TSSAEE
Sbjct: 61  SSDGKRAGKVHSVSGSANHVDGLNGERNYSIEHVQLIRQIKTAKDYYKILGVEKTSSAEE 120

Query: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTSRRQYDHTALVDQYEYNQQH 180
           IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTSRRQYDHTALVDQYEYNQQH
Sbjct: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTSRRQYDHTALVDQYEYNQQH 180

Query: 181 NVRRRRTGRDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYSTGGARSQRRTESDGGGPNL 240
           NVRRRRTGRDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYSTGGARSQRRTESDGGGPNL
Sbjct: 181 NVRRRRTGRDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYSTGGARSQRRTESDGGGPNL 240

Query: 241 LILLLMLPFLLIVLLAYMPFPEPDYSLHKNLSYSIPMATEKHGVEFFVKSSDFDERYPLG 300
           LI LLMLPFLLIVLLAYMPFPEPDYSLHKNLSYSIPMATEKHGVEFFVKSSDFDERYPLG
Sbjct: 241 LIFLLMLPFLLIVLLAYMPFPEPDYSLHKNLSYSIPMATEKHGVEFFVKSSDFDERYPLG 300

Query: 301 SGARSEIESSVIRDYKNMVWRYCHVELQRRKWNKNLPTPHCEKMNNLGFA 350
           SGARSEIESSVIRDYKNMVWRYCHVELQRRKWNKNLPTPHCEKMNNLGFA
Sbjct: 301 SGARSEIESSVIRDYKNMVWRYCHVELQRRKWNKNLPTPHCEKMNNLGFA 350

BLAST of Csor.00g259720 vs. NCBI nr
Match: XP_023006305.1 (chaperone protein dnaJ 49 [Cucurbita maxima] >XP_023006306.1 chaperone protein dnaJ 49 [Cucurbita maxima])

HSP 1 Score: 682 bits (1761), Expect = 2.53e-247
Identity = 341/350 (97.43%), Postives = 345/350 (98.57%), Query Frame = 0

Query: 1   MDGNKDEALRCIRIAEEAIASGNKERALKFIKIACRLNRSLGVDELLSACEEIDLKSPSS 60
           MDGNKDEALRCIRIAEEAIASGNKERALKFIKIACRLNRSL VDELLSACEEID KSPSS
Sbjct: 1   MDGNKDEALRCIRIAEEAIASGNKERALKFIKIACRLNRSLEVDELLSACEEIDSKSPSS 60

Query: 61  SSDGKRAGKVHSVSGSANHVDGLNGERNYSIEHVQLIRQIKTAKDYYKILGVERTSSAEE 120
           SSDGKR GKVHSVSGSANHVDGLNGERNYSIE+VQLIRQIKTAKDYYKILGVE+TSSAEE
Sbjct: 61  SSDGKRTGKVHSVSGSANHVDGLNGERNYSIENVQLIRQIKTAKDYYKILGVEKTSSAEE 120

Query: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTSRRQYDHTALVDQYEYNQQH 180
           IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTSRRQYDHTALVDQYEYNQQH
Sbjct: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTSRRQYDHTALVDQYEYNQQH 180

Query: 181 NVRRRRTGRDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYSTGGARSQRRTESDGGGPNL 240
           NVRRRRTGRDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYSTGGARSQRRTESDGGGPNL
Sbjct: 181 NVRRRRTGRDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYSTGGARSQRRTESDGGGPNL 240

Query: 241 LILLLMLPFLLIVLLAYMPFPEPDYSLHKNLSYSIPMATEKHGVEFFVKSSDFDERYPLG 300
           L+LLLMLPFLLIVLLAYMPFPEPDYSLHKNLSY IPMATEKHGVEFFVKSSDFDERYPLG
Sbjct: 241 LVLLLMLPFLLIVLLAYMPFPEPDYSLHKNLSYIIPMATEKHGVEFFVKSSDFDERYPLG 300

Query: 301 SGARSEIESSVIRDYKNMVWRYCHVELQRRKWNKNLPTPHCEKMNNLGFA 350
           S ARSEIESSVIRDYK+MVWRYCHVELQRRKWNKNLPTPHCEKMNNLGFA
Sbjct: 301 SRARSEIESSVIRDYKSMVWRYCHVELQRRKWNKNLPTPHCEKMNNLGFA 350

BLAST of Csor.00g259720 vs. ExPASy TrEMBL
Match: A0A6J1H4U8 (chaperone protein dnaJ 49 OS=Cucurbita moschata OX=3662 GN=LOC111460072 PE=4 SV=1)

HSP 1 Score: 689 bits (1778), Expect = 3.14e-250
Identity = 343/350 (98.00%), Postives = 348/350 (99.43%), Query Frame = 0

Query: 1   MDGNKDEALRCIRIAEEAIASGNKERALKFIKIACRLNRSLGVDELLSACEEIDLKSPSS 60
           MDGNKDEALRCIRIAEEAIASGNKERALKFIKIACRLNRSL V+ELLSACE+ID KSPSS
Sbjct: 1   MDGNKDEALRCIRIAEEAIASGNKERALKFIKIACRLNRSLEVNELLSACEDIDSKSPSS 60

Query: 61  SSDGKRAGKVHSVSGSANHVDGLNGERNYSIEHVQLIRQIKTAKDYYKILGVERTSSAEE 120
           SSDGKRAGKVHSVSGSANHVDGLNGERNYSIEHVQLIRQIKTAKDYYKILGVE+TSSAEE
Sbjct: 61  SSDGKRAGKVHSVSGSANHVDGLNGERNYSIEHVQLIRQIKTAKDYYKILGVEKTSSAEE 120

Query: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTSRRQYDHTALVDQYEYNQQH 180
           IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLS+DTSRRQYDHTALVDQYEYNQQH
Sbjct: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSNDTSRRQYDHTALVDQYEYNQQH 180

Query: 181 NVRRRRTGRDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYSTGGARSQRRTESDGGGPNL 240
           NVRRRRTGRDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYSTGGARSQRRTESDGGGPNL
Sbjct: 181 NVRRRRTGRDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYSTGGARSQRRTESDGGGPNL 240

Query: 241 LILLLMLPFLLIVLLAYMPFPEPDYSLHKNLSYSIPMATEKHGVEFFVKSSDFDERYPLG 300
           LILLLMLPFLLIVLLAYMPFPEPDYSLHKNLSYSIPMATEKHGVEFFVKSSDFDERYPLG
Sbjct: 241 LILLLMLPFLLIVLLAYMPFPEPDYSLHKNLSYSIPMATEKHGVEFFVKSSDFDERYPLG 300

Query: 301 SGARSEIESSVIRDYKNMVWRYCHVELQRRKWNKNLPTPHCEKMNNLGFA 350
           SGARSEIESSVIRDYKNMVWRYCH+ELQRRKWNKNLPTPHCEKMNNLGFA
Sbjct: 301 SGARSEIESSVIRDYKNMVWRYCHIELQRRKWNKNLPTPHCEKMNNLGFA 350

BLAST of Csor.00g259720 vs. ExPASy TrEMBL
Match: A0A6J1KXF3 (chaperone protein dnaJ 49 OS=Cucurbita maxima OX=3661 GN=LOC111499080 PE=4 SV=1)

HSP 1 Score: 682 bits (1761), Expect = 1.23e-247
Identity = 341/350 (97.43%), Postives = 345/350 (98.57%), Query Frame = 0

Query: 1   MDGNKDEALRCIRIAEEAIASGNKERALKFIKIACRLNRSLGVDELLSACEEIDLKSPSS 60
           MDGNKDEALRCIRIAEEAIASGNKERALKFIKIACRLNRSL VDELLSACEEID KSPSS
Sbjct: 1   MDGNKDEALRCIRIAEEAIASGNKERALKFIKIACRLNRSLEVDELLSACEEIDSKSPSS 60

Query: 61  SSDGKRAGKVHSVSGSANHVDGLNGERNYSIEHVQLIRQIKTAKDYYKILGVERTSSAEE 120
           SSDGKR GKVHSVSGSANHVDGLNGERNYSIE+VQLIRQIKTAKDYYKILGVE+TSSAEE
Sbjct: 61  SSDGKRTGKVHSVSGSANHVDGLNGERNYSIENVQLIRQIKTAKDYYKILGVEKTSSAEE 120

Query: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTSRRQYDHTALVDQYEYNQQH 180
           IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTSRRQYDHTALVDQYEYNQQH
Sbjct: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTSRRQYDHTALVDQYEYNQQH 180

Query: 181 NVRRRRTGRDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYSTGGARSQRRTESDGGGPNL 240
           NVRRRRTGRDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYSTGGARSQRRTESDGGGPNL
Sbjct: 181 NVRRRRTGRDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYSTGGARSQRRTESDGGGPNL 240

Query: 241 LILLLMLPFLLIVLLAYMPFPEPDYSLHKNLSYSIPMATEKHGVEFFVKSSDFDERYPLG 300
           L+LLLMLPFLLIVLLAYMPFPEPDYSLHKNLSY IPMATEKHGVEFFVKSSDFDERYPLG
Sbjct: 241 LVLLLMLPFLLIVLLAYMPFPEPDYSLHKNLSYIIPMATEKHGVEFFVKSSDFDERYPLG 300

Query: 301 SGARSEIESSVIRDYKNMVWRYCHVELQRRKWNKNLPTPHCEKMNNLGFA 350
           S ARSEIESSVIRDYK+MVWRYCHVELQRRKWNKNLPTPHCEKMNNLGFA
Sbjct: 301 SRARSEIESSVIRDYKSMVWRYCHVELQRRKWNKNLPTPHCEKMNNLGFA 350

BLAST of Csor.00g259720 vs. ExPASy TrEMBL
Match: A0A0A0KG46 (J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G306340 PE=4 SV=1)

HSP 1 Score: 588 bits (1517), Expect = 1.72e-210
Identity = 296/352 (84.09%), Postives = 318/352 (90.34%), Query Frame = 0

Query: 1   MDGNKDEALRCIRIAEEAIASGNKERALKFIKIACRLNRSLGVDELLSACEEIDLKSPSS 60
           MDGNKDEALRCIRIAEE+IASGNKERAL+FIKIA RLN+S+ VDELL+ACEEI     S 
Sbjct: 1   MDGNKDEALRCIRIAEESIASGNKERALRFIKIARRLNQSVQVDELLAACEEIG----SG 60

Query: 61  SSDGKRAGKVHSVSGSANHVDGLNGERNYSIEHVQLIRQIKTAKDYYKILGVERTSSAEE 120
           SS+ KRAGK  SVSGS  H DGLNGERNYS+EHVQLIRQIKT KDYY ILGVE+TSSAEE
Sbjct: 61  SSEEKRAGKGESVSGSVKHGDGLNGERNYSMEHVQLIRQIKTTKDYYGILGVEKTSSAEE 120

Query: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTSRRQYDHTALVDQYEYNQQH 180
           IKRAY+KLSLKVHPDKNKAPGS+EAFKKLSKAF CLSDDT RRQYDHT LVDQYEYNQQH
Sbjct: 121 IKRAYRKLSLKVHPDKNKAPGSEEAFKKLSKAFSCLSDDTLRRQYDHTPLVDQYEYNQQH 180

Query: 181 NVR--RRRTGRDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYSTGGARSQRRTESDGGGP 240
           NVR  RRR G DLFEENFDPDEIFRAFFGQGNMFQTSRAYTY TGGA SQ+RTES GGGP
Sbjct: 181 NVRQRRRRNGHDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYRTGGAGSQQRTESYGGGP 240

Query: 241 NLLILLLMLPFLLIVLLAYMPFPEPDYSLHKNLSYSIPMATEKHGVEFFVKSSDFDERYP 300
           N LI+LLMLPFLLI LLAYMPFPEP+Y+LHK+LSYSIPMATEKHGVEFFVKSSDFDERYP
Sbjct: 241 NFLIILLMLPFLLICLLAYMPFPEPEYALHKSLSYSIPMATEKHGVEFFVKSSDFDERYP 300

Query: 301 LGSGARSEIESSVIRDYKNMVWRYCHVELQRRKWNKNLPTPHCEKMNNLGFA 350
           LGS  R E+E+SV+RDY+NMVWRYCH+ELQRR+WNKNLPTPHCEK+N L  A
Sbjct: 301 LGSPGRVELENSVLRDYRNMVWRYCHIELQRRQWNKNLPTPHCEKLNTLAVA 348

BLAST of Csor.00g259720 vs. ExPASy TrEMBL
Match: A0A6J1CB72 (chaperone protein dnaJ 49 OS=Momordica charantia OX=3673 GN=LOC111009903 PE=4 SV=1)

HSP 1 Score: 588 bits (1515), Expect = 6.75e-210
Identity = 295/348 (84.77%), Postives = 318/348 (91.38%), Query Frame = 0

Query: 1   MDGNKDEALRCIRIAEEAIASGNKERALKFIKIACRLNRSLGVDELLSACEEIDLKSPSS 60
           MDGNKDEALRCI IAE AIASGNK RAL+FIKIA RLNRSL VDELL ACEE+D  SP+S
Sbjct: 1   MDGNKDEALRCIHIAEAAIASGNKARALRFIKIARRLNRSLQVDELLKACEEVDSGSPTS 60

Query: 61  SSDGKRAGKVHSVSGSANHVDGLNGERNYSIEHVQLIRQIKTAKDYYKILGVERTSSAEE 120
           SSD KRAGKV SV GSA HV+GLNGERNY+ EHV+LIRQI T KDYY+ILGVE+TSSAEE
Sbjct: 61  SSDEKRAGKVESVPGSAKHVEGLNGERNYTTEHVRLIRQINTTKDYYRILGVEKTSSAEE 120

Query: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTSRRQYDHTALVDQYEYNQQH 180
           IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTSRRQYDHT LVDQYEYNQQH
Sbjct: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTSRRQYDHTDLVDQYEYNQQH 180

Query: 181 NVR--RRRTGRDLFEENFDPDEIFRAFFGQGNMFQTS-RAYTYSTGGARSQRRTESDGGG 240
           NVR  RRRTG DLFEENFDP+EIFRAFFGQGNMF +S RAYTY TGG  SQ+R ES+GGG
Sbjct: 181 NVRQRRRRTGHDLFEENFDPEEIFRAFFGQGNMFHSSSRAYTYRTGGGGSQQRAESNGGG 240

Query: 241 PNLLILLLMLPFLLIVLLAYMPFPEPDYSLHKNLSYSIPMATEKHGVEFFVKSSDFDERY 300
           PNLLILLLMLPFLLI LLAY+PFPEPDYSLH NLSY+IPMATEKHGVEF+VKSSDFD +Y
Sbjct: 241 PNLLILLLMLPFLLIFLLAYLPFPEPDYSLHTNLSYTIPMATEKHGVEFYVKSSDFDVKY 300

Query: 301 PLGSGARSEIESSVIRDYKNMVWRYCHVELQRRKWNKNLPTPHCEKMN 345
           P GS AR+EIE++V+RDYK+MVWRYCH+ELQRR+WNKNLPTPHCEKMN
Sbjct: 301 PPGSLARAEIENTVLRDYKSMVWRYCHIELQRRQWNKNLPTPHCEKMN 348

BLAST of Csor.00g259720 vs. ExPASy TrEMBL
Match: A0A1S4E2R1 (chaperone protein dnaJ 49-like OS=Cucumis melo OX=3656 GN=LOC103497850 PE=4 SV=1)

HSP 1 Score: 584 bits (1505), Expect = 1.16e-208
Identity = 294/352 (83.52%), Postives = 317/352 (90.06%), Query Frame = 0

Query: 1   MDGNKDEALRCIRIAEEAIASGNKERALKFIKIACRLNRSLGVDELLSACEEIDLKSPSS 60
           MDGNKDEALRCIRIAEE+IASGNKERAL+FIKIA RLN+SL +DELL+ACEE+     S 
Sbjct: 1   MDGNKDEALRCIRIAEESIASGNKERALRFIKIARRLNQSLQIDELLTACEEMG----SG 60

Query: 61  SSDGKRAGKVHSVSGSANHVDGLNGERNYSIEHVQLIRQIKTAKDYYKILGVERTSSAEE 120
           SS  KRAGK  SVSGS  HVDGLNGERNYS+EHVQLIRQIKT  DYY ILGVE+TSSAEE
Sbjct: 61  SSAEKRAGKGESVSGSVKHVDGLNGERNYSMEHVQLIRQIKTTMDYYGILGVEKTSSAEE 120

Query: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTSRRQYDHTALVDQYEYNQQH 180
           IKRAY+KLSLKVHPDKNKAPGS+EAFKKLSKAF CLSDDT RR+YD TALVDQYEYNQQH
Sbjct: 121 IKRAYRKLSLKVHPDKNKAPGSEEAFKKLSKAFSCLSDDTLRRRYDQTALVDQYEYNQQH 180

Query: 181 NVR--RRRTGRDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYSTGGARSQRRTESDGGGP 240
           NVR  RRR G DLFEENFDPDEIFRAFFGQGNMFQTSRAYTY TGGA SQ+RTESDGGGP
Sbjct: 181 NVRHRRRRNGHDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYRTGGAGSQQRTESDGGGP 240

Query: 241 NLLILLLMLPFLLIVLLAYMPFPEPDYSLHKNLSYSIPMATEKHGVEFFVKSSDFDERYP 300
           + LI+LLMLPFLLI LLAYMPFPEP+Y+LHKNLSYSIPMATEKHGVEFFVKSSDF+ RYP
Sbjct: 241 SFLIILLMLPFLLICLLAYMPFPEPEYALHKNLSYSIPMATEKHGVEFFVKSSDFNVRYP 300

Query: 301 LGSGARSEIESSVIRDYKNMVWRYCHVELQRRKWNKNLPTPHCEKMNNLGFA 350
           LGS  R EIE+SV+RDY+NMVWRYCH+ELQRR+WNKNLPTPHCEK+N L  A
Sbjct: 301 LGSPGRVEIENSVLRDYRNMVWRYCHIELQRRQWNKNLPTPHCEKLNTLAVA 348

BLAST of Csor.00g259720 vs. TAIR 10
Match: AT5G49060.1 (Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977) )

HSP 1 Score: 348.2 bits (892), Expect = 7.7e-96
Identity = 184/355 (51.83%), Postives = 256/355 (72.11%), Query Frame = 0

Query: 1   MDGNKDEALRCIRIAEEAIASGNKERALKFIKIACRLNRSLGVDELLSACEEIDLKSPSS 60
           MDGNKD+A RC+RIAE+AI SG+KERALKFI +A RLN SL VDEL++AC+ +D  S +S
Sbjct: 1   MDGNKDDASRCLRIAEDAIVSGDKERALKFINMAKRLNPSLSVDELVAACDNLDSVSRNS 60

Query: 61  SSDGKRAGKVHSVSGSANHVDGLNGERNYSIEHVQLIRQIKTAKDYYKILGVERTSSAEE 120
           S     + K+ ++ G  + ++   G+  Y+ E+V L+R I    DYY ILG+E+  S +E
Sbjct: 61  SV----SEKLKTMDGDDDKLE--TGKMKYTEENVDLVRNIIRNNDYYAILGLEKNCSVDE 120

Query: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTSRRQYDHTALVDQYEYNQQH 180
           I++AY+KLSLKVHPDKNKAPGS+EAFKK+SKAF CLSD  SRRQ+D   +VD++++ Q+ 
Sbjct: 121 IRKAYRKLSLKVHPDKNKAPGSEEAFKKVSKAFTCLSDGNSRRQFDQVGIVDEFDHVQRR 180

Query: 181 NVRRRR---TGRDLFEENFDPDEIFRAFFG-QGNMFQTSRAYTYSTGGARSQ-RRTESDG 240
           N R RR   T  D F++ FDP+EIFR  FG Q  +F+ S A  Y T   R+Q R  E + 
Sbjct: 181 NRRPRRRYNTRNDFFDDEFDPEEIFRTVFGQQREVFRASHA--YRTRQPRNQFREEEINV 240

Query: 241 GGPNLLILLLMLPFLLIVLLAYMPFPEPDYSLHKNLSYSIPMATEKHGVEFFVKS-SDFD 300
            GP+ L ++ +LPF L++LLAY+PF EPDYSLHKN SY IP  T+   + F+V+S S FD
Sbjct: 241 AGPSCLTIIQILPFFLLLLLAYLPFSEPDYSLHKNQSYQIPKTTQNTEISFYVRSASAFD 300

Query: 301 ERYPLGSGARSEIESSVIRDYKNMVWRYCHVELQRRKWNKNLPTPHCEKMNNLGF 350
           E++PL S AR+ +E +VI++YK+ +++ C +ELQ+R+WNK +PTPHC ++ + GF
Sbjct: 301 EKFPLSSSARANLEGNVIKEYKHFLFQSCRIELQKRRWNKKIPTPHCIELQDRGF 347

BLAST of Csor.00g259720 vs. TAIR 10
Match: AT3G57340.1 (Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977) )

HSP 1 Score: 245.4 bits (625), Expect = 7.0e-65
Identity = 141/367 (38.42%), Postives = 217/367 (59.13%), Query Frame = 0

Query: 1   MDGNKDEALRCIRIAEEAIASGNKERALKFIKIACRLNRSLGVDELLS------ACEEID 60
           MDGNKD+AL+C++I + A+ +G++ RALKF+  A RL+ +L +D+L+S      + E   
Sbjct: 1   MDGNKDDALKCLKICKSAMEAGDRPRALKFLAKARRLDPNLPIDDLVSELNNNKSDEPGS 60

Query: 61  LKSPSSSS--DGKRAGKVHSVSGSANHVDGLNGERNYSIEHVQLIRQIKTAKDYYKILGV 120
            KSP S++  D   +    S+    +     +   +Y+ E + ++R+IK+ KDYY+ILG+
Sbjct: 61  AKSPGSAAAKDSSNSSDRPSLRQRGSSTTSSSSSMSYTEEQISIVRKIKSKKDYYEILGL 120

Query: 121 ERTSSAEEIKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTSRRQYDHTALVD 180
           E   S +++++AY+KLSLKVHPDKN+APGS+EAFK +SKAF CLS+D +R++YD +   D
Sbjct: 121 ESNCSVDDVRKAYRKLSLKVHPDKNQAPGSEEAFKSVSKAFQCLSNDEARKKYDVSG-SD 180

Query: 181 QYEYNQQHNVRRRR-TGRDLFEENFDPDEIFRAFFGQGNM--------FQTSRAYTYSTG 240
           +  Y  + + R     G   +E+ FDP+EIFR+FFG G              R++ +   
Sbjct: 181 EPIYQPRRSARSNGFNGGYYYEDEFDPNEIFRSFFGGGGFGGGGMPPATAQFRSFNFGAT 240

Query: 241 GARSQRRTESDGGGPNLLILLLMLPFLLIVLLAYMPFPEPDYSLHKNLSYSIPMATEKHG 300
             R+    ++   G N  ILL +LP + I+LL +MP  +P Y L     Y     T+K G
Sbjct: 241 RQRTANNNQAPDAGFNARILLQLLPVVFILLLNFMPSSQPVYQLSATYPYQYKFTTQK-G 300

Query: 301 VEFFVKSSDFDERYPLGSGARSEIESSVIRDYKNMVWRYCHVELQRRKWNKNLPTPHCEK 351
           V +FVKSS F++ YP  S  R  +E  V RDY +++ + C  E+QR++W     TPHC+ 
Sbjct: 301 VNYFVKSSKFEQDYPRDSNDRHTLEEQVERDYVSILSQNCRYEMQRKQWGFVRETPHCDM 360

BLAST of Csor.00g259720 vs. TAIR 10
Match: AT3G57340.2 (Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977) )

HSP 1 Score: 245.4 bits (625), Expect = 7.0e-65
Identity = 141/367 (38.42%), Postives = 217/367 (59.13%), Query Frame = 0

Query: 1   MDGNKDEALRCIRIAEEAIASGNKERALKFIKIACRLNRSLGVDELLS------ACEEID 60
           MDGNKD+AL+C++I + A+ +G++ RALKF+  A RL+ +L +D+L+S      + E   
Sbjct: 1   MDGNKDDALKCLKICKSAMEAGDRPRALKFLAKARRLDPNLPIDDLVSELNNNKSDEPGS 60

Query: 61  LKSPSSSS--DGKRAGKVHSVSGSANHVDGLNGERNYSIEHVQLIRQIKTAKDYYKILGV 120
            KSP S++  D   +    S+    +     +   +Y+ E + ++R+IK+ KDYY+ILG+
Sbjct: 61  AKSPGSAAAKDSSNSSDRPSLRQRGSSTTSSSSSMSYTEEQISIVRKIKSKKDYYEILGL 120

Query: 121 ERTSSAEEIKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTSRRQYDHTALVD 180
           E   S +++++AY+KLSLKVHPDKN+APGS+EAFK +SKAF CLS+D +R++YD +   D
Sbjct: 121 ESNCSVDDVRKAYRKLSLKVHPDKNQAPGSEEAFKSVSKAFQCLSNDEARKKYDVSG-SD 180

Query: 181 QYEYNQQHNVRRRR-TGRDLFEENFDPDEIFRAFFGQGNM--------FQTSRAYTYSTG 240
           +  Y  + + R     G   +E+ FDP+EIFR+FFG G              R++ +   
Sbjct: 181 EPIYQPRRSARSNGFNGGYYYEDEFDPNEIFRSFFGGGGFGGGGMPPATAQFRSFNFGAT 240

Query: 241 GARSQRRTESDGGGPNLLILLLMLPFLLIVLLAYMPFPEPDYSLHKNLSYSIPMATEKHG 300
             R+    ++   G N  ILL +LP + I+LL +MP  +P Y L     Y     T+K G
Sbjct: 241 RQRTANNNQAPDAGFNARILLQLLPVVFILLLNFMPSSQPVYQLSATYPYQYKFTTQK-G 300

Query: 301 VEFFVKSSDFDERYPLGSGARSEIESSVIRDYKNMVWRYCHVELQRRKWNKNLPTPHCEK 351
           V +FVKSS F++ YP  S  R  +E  V RDY +++ + C  E+QR++W     TPHC+ 
Sbjct: 301 VNYFVKSSKFEQDYPRDSNDRHTLEEQVERDYVSILSQNCRYEMQRKQWGFVRETPHCDM 360

BLAST of Csor.00g259720 vs. TAIR 10
Match: AT5G05750.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 195.3 bits (495), Expect = 8.3e-50
Identity = 111/283 (39.22%), Postives = 177/283 (62.54%), Query Frame = 0

Query: 1   MDGNKDEALRCIRIAEEAIASGNKERALKFIKIACRLNRSLGVDELLSAC-EEIDLKSPS 60
           MDGNKD+AL+C++I ++AI +G++ RALKF++ A RL+ +L +D L+S   ++ D  +  
Sbjct: 1   MDGNKDDALKCLKIGKDAIEAGDRSRALKFLEKARRLDPNLPIDGLVSDLKKQSDEPAAE 60

Query: 61  SSSDGKRAGKVHSVS--------GSANHVDGLNGERNYSIEHVQLIRQIKTAKDYYKILG 120
             S G  A +    S        GS++   G +   + + E   ++R+IK+ KDYY+ILG
Sbjct: 61  EDSPGSAANESSKPSDRPSLRQRGSSSSAAGSSSSSSSTEEQRTIVREIKSKKDYYEILG 120

Query: 121 VERTSSAEEIKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTSRRQYDHTALV 180
           ++   S E+++++Y+KLSLKVHPDKNKAPGS+EAFK +SKAF CLS++ +RR+YD +   
Sbjct: 121 LKSNCSVEDLRKSYRKLSLKVHPDKNKAPGSEEAFKSVSKAFQCLSNEDTRRKYDGSG-S 180

Query: 181 DQYEYNQQHNVRRRRTGRDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYSTGGARSQRRT 240
           D+  Y  + + RR       +++ FD DEIFR+FFG G M   +  +     G  ++   
Sbjct: 181 DEPAYQPRRDARRNNGFNGFYDDEFDADEIFRSFFGGGEMNPATTQFRSFNFGGGTRTAN 240

Query: 241 ESDGGGPNLLILLLMLPFLLIVLLAYMPFPEPDYSLHKNLSYS 275
           ++   G N  +LL +LP + I+LL ++P P+P YSL   ++ S
Sbjct: 241 QASDTGFNPRVLLQILPVVFILLLNFLPSPQPIYSLSHRITTS 282

BLAST of Csor.00g259720 vs. TAIR 10
Match: AT3G06778.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 70.5 bits (171), Expect = 3.1e-12
Identity = 32/74 (43.24%), Postives = 49/74 (66.22%), Query Frame = 0

Query: 93  HVQLIRQIKTAKDYYKILGVERTSSAEEIKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKA 152
           H+  I       D+Y ILG++  +  + I++ Y KL+LKVHPDKN  P +D AFK + +A
Sbjct: 30  HINRISSGSCFIDWYLILGIQEDAEVKVIRKRYHKLALKVHPDKNNHPKADIAFKLIHEA 89

Query: 153 FMCLSDDTSRRQYD 167
           ++CLSD+T RR ++
Sbjct: 90  YLCLSDETKRRSFN 103

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FH281.1e-9451.83Chaperone protein dnaJ 49 OS=Arabidopsis thaliana OX=3702 GN=ATJ49 PE=2 SV=2[more]
Q28I387.1e-3833.63DnaJ homolog subfamily B member 14 OS=Xenopus tropicalis OX=8364 GN=dnajb14 PE=2... [more]
Q7ZXQ86.0e-3731.75DnaJ homolog subfamily B member 14 OS=Xenopus laevis OX=8355 GN=dnajb14 PE=2 SV=... [more]
Q9QYI45.1e-3633.04DnaJ homolog subfamily B member 12 OS=Mus musculus OX=10090 GN=Dnajb12 PE=1 SV=2[more]
Q149L67.4e-3531.55DnaJ homolog subfamily B member 14 OS=Mus musculus OX=10090 GN=Dnajb14 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
KAG6575070.11.22e-254100.00Chaperone protein dnaJ 49, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7013644.17.06e-25499.71Chaperone protein dnaJ 49 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022958924.16.49e-25098.00chaperone protein dnaJ 49 [Cucurbita moschata] >XP_022958925.1 chaperone protein... [more]
XP_023547565.11.86e-24998.00chaperone protein dnaJ 49 [Cucurbita pepo subsp. pepo] >XP_023547566.1 chaperone... [more]
XP_023006305.12.53e-24797.43chaperone protein dnaJ 49 [Cucurbita maxima] >XP_023006306.1 chaperone protein d... [more]
Match NameE-valueIdentityDescription
A0A6J1H4U83.14e-25098.00chaperone protein dnaJ 49 OS=Cucurbita moschata OX=3662 GN=LOC111460072 PE=4 SV=... [more]
A0A6J1KXF31.23e-24797.43chaperone protein dnaJ 49 OS=Cucurbita maxima OX=3661 GN=LOC111499080 PE=4 SV=1[more]
A0A0A0KG461.72e-21084.09J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G306340 PE=4 SV=1[more]
A0A6J1CB726.75e-21084.77chaperone protein dnaJ 49 OS=Momordica charantia OX=3673 GN=LOC111009903 PE=4 SV... [more]
A0A1S4E2R11.16e-20883.52chaperone protein dnaJ 49-like OS=Cucumis melo OX=3656 GN=LOC103497850 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G49060.17.7e-9651.83Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977) [more]
AT3G57340.17.0e-6538.42Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977) [more]
AT3G57340.27.0e-6538.42Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977) [more]
AT5G05750.18.3e-5039.22DNAJ heat shock N-terminal domain-containing protein [more]
AT3G06778.13.1e-1243.24Chaperone DnaJ-domain superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001623DnaJ domainPRINTSPR00625JDOMAINcoord: 141..161
score: 31.87
coord: 107..125
score: 51.54
coord: 125..140
score: 51.05
IPR001623DnaJ domainSMARTSM00271dnaj_3coord: 104..161
e-value: 4.7E-25
score: 99.2
IPR001623DnaJ domainPFAMPF00226DnaJcoord: 105..166
e-value: 8.9E-24
score: 83.4
IPR001623DnaJ domainPROSITEPS50076DNAJ_2coord: 105..169
score: 19.47489
IPR001623DnaJ domainCDDcd06257DnaJcoord: 105..158
e-value: 1.25869E-20
score: 82.2081
IPR036869Chaperone J-domain superfamilyGENE3D1.10.287.110DnaJ domaincoord: 102..218
e-value: 4.3E-29
score: 103.3
IPR036869Chaperone J-domain superfamilySUPERFAMILY46565Chaperone J-domaincoord: 101..212
IPR015399Domain of unknown function DUF1977, DnaJ-likePFAMPF09320DUF1977coord: 261..331
e-value: 6.6E-8
score: 32.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 55..82
NoneNo IPR availablePANTHERPTHR43908AT29763P-RELATEDcoord: 1..349
NoneNo IPR availablePANTHERPTHR43908:SF5CHAPERONE PROTEIN DNAJ 49coord: 1..349

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g259720.m01Csor.00g259720.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071218 cellular response to misfolded protein
biological_process GO:0051085 chaperone cofactor-dependent protein refolding
biological_process GO:0030433 ubiquitin-dependent ERAD pathway
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0030544 Hsp70 protein binding