Csor.00g245880 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g245880
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionPlant invertase/pectin methylesterase inhibitor superfamily protein
LocationCsor_Chr16: 869312 .. 869995 (-)
RNA-Seq ExpressionCsor.00g245880
SyntenyCsor.00g245880
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintronterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAATCCTTGCTAATCCTCTTCTTCTTCATTTTAACTGCCACATTCACCACCTCCGCCGTGGCTTCTTCTCCCTCCGCCGTGGCTTCTTCTTCCTCCGCCATTCAGCCGGTGAAACCACACATCAAAAAGGCCTGCAGGCCGACGCCATATCCACGCCTTTGCGAGACCGCGCTCTCCTTATATTCTTCACAAACCAAAACAACCCAACAAGAGCTTTGTAGGGCTGCCGTGGTTTCTTCCCTCAAAGCTGCACAAAACGCCACCTCCATAATCTCCAAATTATCTCGCCGGAAGGTGTCGCCCTATGAAGCCGAGGTCATCGGAGATTGCATCGATAATCTCAAAGACTCCATCTACGAGCTTCGTAGAGCTACCACTGCTATCAAAAGCTTGAATCGTGCTAACGACCTGAAGTTTCAGGTTGTTAATCATAAACTTTCAATTTCATCAAATTATTTTAAATTAGATTCTAATGAATATGTTCTTAACCAGTTGAGTAGTATCAAGACGTGGTTGAGCGCGGCAGGGACCGACATTGTGACATGCACCGATGGGTTGAGCGGGGGCGCGGGGTGGAAGGTGAGATCGACGGTGAAGAAAGAGGTGAAGAAATGTAGTGTGAATGTAATAAGGCAGACTAGTAACGCCTTGTCTCTCATTAGCAACTTGAAGTACAAATGA

mRNA sequence

ATGAAATCCTTGCTAATCCTCTTCTTCTTCATTTTAACTGCCACATTCACCACCTCCGCCGTGGCTTCTTCTCCCTCCGCCGTGGCTTCTTCTTCCTCCGCCATTCAGCCGGTGAAACCACACATCAAAAAGGCCTGCAGGCCGACGCCATATCCACGCCTTTGCGAGACCGCGCTCTCCTTATATTCTTCACAAACCAAAACAACCCAACAAGAGCTTTGTAGGGCTGCCGTGGTTTCTTCCCTCAAAGCTGCACAAAACGCCACCTCCATAATCTCCAAATTATCTCGCCGGAAGGTGTCGCCCTATGAAGCCGAGGTCATCGGAGATTGCATCGATAATCTCAAAGACTCCATCTACGAGCTTCGTAGAGCTACCACTGCTATCAAAAGCTTGAATCGTGCTAACGACCTGAAGTTTCAGTTGAGTAGTATCAAGACGTGGTTGAGCGCGGCAGGGACCGACATTGTGACATGCACCGATGGGTTGAGCGGGGGCGCGGGGTGGAAGGTGAGATCGACGGTGAAGAAAGAGGTGAAGAAATGTAGTGTGAATGTAATAAGGCAGACTAGTAACGCCTTGTCTCTCATTAGCAACTTGAAGTACAAATGA

Coding sequence (CDS)

ATGAAATCCTTGCTAATCCTCTTCTTCTTCATTTTAACTGCCACATTCACCACCTCCGCCGTGGCTTCTTCTCCCTCCGCCGTGGCTTCTTCTTCCTCCGCCATTCAGCCGGTGAAACCACACATCAAAAAGGCCTGCAGGCCGACGCCATATCCACGCCTTTGCGAGACCGCGCTCTCCTTATATTCTTCACAAACCAAAACAACCCAACAAGAGCTTTGTAGGGCTGCCGTGGTTTCTTCCCTCAAAGCTGCACAAAACGCCACCTCCATAATCTCCAAATTATCTCGCCGGAAGGTGTCGCCCTATGAAGCCGAGGTCATCGGAGATTGCATCGATAATCTCAAAGACTCCATCTACGAGCTTCGTAGAGCTACCACTGCTATCAAAAGCTTGAATCGTGCTAACGACCTGAAGTTTCAGTTGAGTAGTATCAAGACGTGGTTGAGCGCGGCAGGGACCGACATTGTGACATGCACCGATGGGTTGAGCGGGGGCGCGGGGTGGAAGGTGAGATCGACGGTGAAGAAAGAGGTGAAGAAATGTAGTGTGAATGTAATAAGGCAGACTAGTAACGCCTTGTCTCTCATTAGCAACTTGAAGTACAAATGA

Protein sequence

MKSLLILFFFILTATFTTSAVASSPSAVASSSSAIQPVKPHIKKACRPTPYPRLCETALSLYSSQTKTTQQELCRAAVVSSLKAAQNATSIISKLSRRKVSPYEAEVIGDCIDNLKDSIYELRRATTAIKSLNRANDLKFQLSSIKTWLSAAGTDIVTCTDGLSGGAGWKVRSTVKKEVKKCSVNVIRQTSNALSLISNLKYK
Homology
BLAST of Csor.00g245880 vs. ExPASy Swiss-Prot
Match: P17407 (21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 6.6e-20
Identity = 65/189 (34.39%), Postives = 108/189 (57.14%), Query Frame = 0

Query: 19  SAVASSPSAVASSSSAIQPVKPHIKKACRPTPYPRLCETALSLYSSQTKTTQQELCRAAV 78
           S +  S   V  ++++  P    IK +C  T YP +CE +LS Y+   +   QEL   A+
Sbjct: 6   STLVFSALLVILAAASAAPANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNNPQELASTAL 65

Query: 79  VSSLKAAQNATSIISKLSRRK-VSPYEAEVIGDCIDNLKDSIYELRRATTAIKSLNRA-- 138
             SL   Q A + + +L++ K +   +   I DC++ ++DS+  + R+   +K+L+ A  
Sbjct: 66  QVSLTRTQQAQTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEMKNLSHAKG 125

Query: 139 NDLKFQLSSIKTWLSAAGTDIVTCTDGLSG-GAGWKVRSTVKKEVKKCSVNVIRQTSNAL 198
           ND  F++S+++TW+SAA TD  TC DG +G G   K++ +V+ +V    V V R TSNAL
Sbjct: 126 NDFTFRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQV----VAVARVTSNAL 185

Query: 199 SLISNLKYK 204
           +L++N   K
Sbjct: 186 ALVNNFAAK 190

BLAST of Csor.00g245880 vs. ExPASy Swiss-Prot
Match: Q9SB38 (Pectinesterase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=PMEI4 PE=2 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 1.9e-19
Identity = 66/198 (33.33%), Postives = 109/198 (55.05%), Query Frame = 0

Query: 6   ILFFFILTATFTTSAVASS-PSAVASSSSAIQPVKPHIKKACRPTPYPRLCETALSLYSS 65
           +L F +L+ T      +S+ P   A+     Q    ++K AC  T YP +C   LS YSS
Sbjct: 1   MLRFVVLSLTLMVFINSSNFPKTAATPPGTYQNHTTYVKTACNSTTYPTMCYNCLSSYSS 60

Query: 66  QTKTTQQELCRAAVVSSLKAAQNATSIISKL--SRRKVSPYEAEVIGDCIDNLKDSIYEL 125
             K+   +LC  ++  ++K+A+NAT ++S L    +    +E  ++ DC+D +KD+I EL
Sbjct: 61  TIKSDPIKLCTTSLNLNVKSAKNATLVVSNLLQKAKAAKSHEVSILKDCVDEMKDTIDEL 120

Query: 126 RRATTAIKSL-NRANDLKFQLSSIKTWLSAAGTDIVTCTDGLSGGAGWKVRSTVKKEVKK 185
           ++A   +K +       +  L ++KTW+S+A TD  TCTDG   G   +V    KK+VKK
Sbjct: 121 KQAVAEMKYVRGGGKTTEEHLKNVKTWVSSALTDEGTCTDGFEEG---RVNVETKKKVKK 180

Query: 186 CSVNVIRQTSNALSLISN 200
               + + TSN L+L+++
Sbjct: 181 AISELSKTTSNTLALLTH 195

BLAST of Csor.00g245880 vs. ExPASy Swiss-Prot
Match: Q84WE4 (Pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PMEI3 PE=2 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 1.1e-14
Identity = 57/167 (34.13%), Postives = 86/167 (51.50%), Query Frame = 0

Query: 42  IKKACRPTPYPRLCETALSLYSSQTKTTQQELCRAAVVSSLKAAQNATS----IISKLSR 101
           +  +C    YP LC   LS YS  T T +++L +AA+  SL  AQ+A      +   + +
Sbjct: 34  VHSSCEHASYPSLCVRTLSSYSGPTITNRRDLAQAAIKISLSHAQSAAKKLAVVRDSVGK 93

Query: 102 RKVSPYEAEVIGDCIDNLKDSIYELRRATTAIKSL----NRANDLKFQLSSIKTWLSAAG 161
           +K    E   + DC++ + DS+ EL R    +K L      A + ++Q+S+ +TW SAA 
Sbjct: 94  KK---QEKAALVDCVEMIGDSVDELSRTLGVLKHLRVSGGSAKEFRWQMSNAQTWASAAL 153

Query: 162 TDIVTCTDGLSGGAGWKVRSTVKKEVKKCSVNVIRQTSNALSLISNL 201
           TD  TC DG  G         +K EVK+    V R TSNAL +++ L
Sbjct: 154 TDDDTCLDGFQG----MDDGEIKTEVKQWMTKVARVTSNALYMVNQL 193

BLAST of Csor.00g245880 vs. ExPASy Swiss-Prot
Match: Q9SI72 (Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 1.1e-14
Identity = 69/206 (33.50%), Postives = 112/206 (54.37%), Query Frame = 0

Query: 9   FFILTATFTTSAVASSPSAVASSSSAIQPVKPHIKKACRPTPYPRLCETALSLYSSQTK- 68
           F ++  + T   +  S SA  + S + Q     I  +C+ T YP LC   LS Y+++ + 
Sbjct: 8   FLVILLSIT---ILQSSSATPNRSESDQ----FIVSSCQTTQYPSLCVHTLSAYATKIRH 67

Query: 69  TTQQELCRAAVVSSLKAAQNATSIISKLSRR----KVSPYEA-----EVIGDCIDNLKDS 128
              Q+L + A++ SL  A++ T  ++KL++     K   Y A     EV+G+ +D L  S
Sbjct: 68  NNDQDLAQTALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQS 127

Query: 129 IYELRRATTAIKSLNRANDLKFQLSSIKTWLSAAGTDIVTCTDGLSGGA-GWKVRSTVKK 188
           + EL RA  A+ S     D  +++S+++TW+SAA TD  TC DG S  A G KV+  ++ 
Sbjct: 128 VKELARAGHAVAS----EDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRY 187

Query: 189 EVKKCSVNVIRQTSNALSLISNLKYK 204
           +V    V+V + TSNAL+L++    K
Sbjct: 188 KV----VHVAQVTSNALALVNQFAEK 198

BLAST of Csor.00g245880 vs. ExPASy Swiss-Prot
Match: Q9SI74 (Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 1.4e-14
Identity = 58/185 (31.35%), Postives = 106/185 (57.30%), Query Frame = 0

Query: 17  TTSAVASSPSAVASSSSAIQPVKPHIKKACRPTPYPRLCETALSLYSSQTKTTQQELCRA 76
           ++S ++S   + +SS+ + Q    +IK +C  T Y  +C  +LS Y+S  ++  Q+L   
Sbjct: 123 SSSPLSSLSPSSSSSTYSNQTNLDYIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVI 182

Query: 77  AVVSSLKAAQNATSIISKLSR-RKVSPYEAEVIGDCIDNLKDSIYELRRATTAIKSLNRA 136
           A+  +L +A++A+  +  +S    ++  E   + DC++ + DS+  L+ +   + S+N  
Sbjct: 183 ALNLTLSSAKSASKFVKNISHGGGLTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINYK 242

Query: 137 NDLKFQ--LSSIKTWLSAAGTDIVTCTDGLSGGAGWKVRSTVKKEVKKCSVNVIRQTSNA 196
           +  KF+  +S ++TW+SAA T+  TC DG S      V++ VK  V++  V V R TSNA
Sbjct: 243 DSAKFEMVMSDVETWVSAALTNDDTCMDGFS-----LVKTAVKDLVRRHVVEVARLTSNA 302

Query: 197 LSLIS 199
           L+LI+
Sbjct: 303 LALIN 302

BLAST of Csor.00g245880 vs. NCBI nr
Match: KAG6576794.1 (hypothetical protein SDJN03_24368, partial [Cucurbita argyrosperma subsp. sororia] >KAG7014821.1 hypothetical protein SDJN02_22450, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 376 bits (965), Expect = 6.56e-131
Identity = 203/203 (100.00%), Postives = 203/203 (100.00%), Query Frame = 0

Query: 1   MKSLLILFFFILTATFTTSAVASSPSAVASSSSAIQPVKPHIKKACRPTPYPRLCETALS 60
           MKSLLILFFFILTATFTTSAVASSPSAVASSSSAIQPVKPHIKKACRPTPYPRLCETALS
Sbjct: 1   MKSLLILFFFILTATFTTSAVASSPSAVASSSSAIQPVKPHIKKACRPTPYPRLCETALS 60

Query: 61  LYSSQTKTTQQELCRAAVVSSLKAAQNATSIISKLSRRKVSPYEAEVIGDCIDNLKDSIY 120
           LYSSQTKTTQQELCRAAVVSSLKAAQNATSIISKLSRRKVSPYEAEVIGDCIDNLKDSIY
Sbjct: 61  LYSSQTKTTQQELCRAAVVSSLKAAQNATSIISKLSRRKVSPYEAEVIGDCIDNLKDSIY 120

Query: 121 ELRRATTAIKSLNRANDLKFQLSSIKTWLSAAGTDIVTCTDGLSGGAGWKVRSTVKKEVK 180
           ELRRATTAIKSLNRANDLKFQLSSIKTWLSAAGTDIVTCTDGLSGGAGWKVRSTVKKEVK
Sbjct: 121 ELRRATTAIKSLNRANDLKFQLSSIKTWLSAAGTDIVTCTDGLSGGAGWKVRSTVKKEVK 180

Query: 181 KCSVNVIRQTSNALSLISNLKYK 203
           KCSVNVIRQTSNALSLISNLKYK
Sbjct: 181 KCSVNVIRQTSNALSLISNLKYK 203

BLAST of Csor.00g245880 vs. NCBI nr
Match: XP_022922937.1 (pectinesterase inhibitor 4-like [Cucurbita moschata])

HSP 1 Score: 367 bits (942), Expect = 2.10e-127
Identity = 197/203 (97.04%), Postives = 201/203 (99.01%), Query Frame = 0

Query: 1   MKSLLILFFFILTATFTTSAVASSPSAVASSSSAIQPVKPHIKKACRPTPYPRLCETALS 60
           MKSLLILFFFILTATFTTSAV SSPSAV SSSSAIQPVKPHIKKACRPTPYPRLCETALS
Sbjct: 1   MKSLLILFFFILTATFTTSAVPSSPSAVPSSSSAIQPVKPHIKKACRPTPYPRLCETALS 60

Query: 61  LYSSQTKTTQQELCRAAVVSSLKAAQNATSIISKLSRRKVSPYEAEVIGDCIDNLKDSIY 120
           LYSSQTKTTQQELCRAAVVSSLKAAQNATSIISKLSRRKVSPYEAEVIGDCIDNLKDS+Y
Sbjct: 61  LYSSQTKTTQQELCRAAVVSSLKAAQNATSIISKLSRRKVSPYEAEVIGDCIDNLKDSVY 120

Query: 121 ELRRATTAIKSLNRANDLKFQLSSIKTWLSAAGTDIVTCTDGLSGGAGWKVRSTVKKEVK 180
           ELRRATTAIKSLNRAN+++FQLSSIKTWLSAAGTDIVTCTDGLSGGAGWKVRSTVKKEVK
Sbjct: 121 ELRRATTAIKSLNRANNVEFQLSSIKTWLSAAGTDIVTCTDGLSGGAGWKVRSTVKKEVK 180

Query: 181 KCSVNVIRQTSNALSLISNLKYK 203
           KCSVNVIRQTSNALSLISNLKYK
Sbjct: 181 KCSVNVIRQTSNALSLISNLKYK 203

BLAST of Csor.00g245880 vs. NCBI nr
Match: XP_023553135.1 (21 kDa protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 360 bits (925), Expect = 8.20e-125
Identity = 194/203 (95.57%), Postives = 199/203 (98.03%), Query Frame = 0

Query: 1   MKSLLILFFFILTATFTTSAVASSPSAVASSSSAIQPVKPHIKKACRPTPYPRLCETALS 60
           MKSLLILFFFILTATFTTSAVASS SAVASSSSAIQPVKPHI+KACRPTPYPRLCETALS
Sbjct: 1   MKSLLILFFFILTATFTTSAVASSSSAVASSSSAIQPVKPHIRKACRPTPYPRLCETALS 60

Query: 61  LYSSQTKTTQQELCRAAVVSSLKAAQNATSIISKLSRRKVSPYEAEVIGDCIDNLKDSIY 120
           LYSSQTK TQQELCRAAVVSSLKAAQNATSIISKLSRRKVSPYEAEVIGDCIDNLKDS+Y
Sbjct: 61  LYSSQTKRTQQELCRAAVVSSLKAAQNATSIISKLSRRKVSPYEAEVIGDCIDNLKDSVY 120

Query: 121 ELRRATTAIKSLNRANDLKFQLSSIKTWLSAAGTDIVTCTDGLSGGAGWKVRSTVKKEVK 180
           ELRRATTAIKSLNRAND++FQLSSIKTWLSA  TDI+TCTDGLSGGAGWKVRSTVKKEVK
Sbjct: 121 ELRRATTAIKSLNRANDVEFQLSSIKTWLSAGETDIMTCTDGLSGGAGWKVRSTVKKEVK 180

Query: 181 KCSVNVIRQTSNALSLISNLKYK 203
           KCSVNVIRQTSNALSLISNLKYK
Sbjct: 181 KCSVNVIRQTSNALSLISNLKYK 203

BLAST of Csor.00g245880 vs. NCBI nr
Match: XP_022985512.1 (pectinesterase inhibitor 4-like [Cucurbita maxima])

HSP 1 Score: 332 bits (852), Expect = 8.55e-114
Identity = 182/203 (89.66%), Postives = 189/203 (93.10%), Query Frame = 0

Query: 1   MKSLLILFFFILTATFTTSAVASSPSAVASSSSAIQPVKPHIKKACRPTPYPRLCETALS 60
           MKSLLILFFFIL ATFT SAVA+S       SSAIQPVKPHI+KACRPTPYPRLCETALS
Sbjct: 1   MKSLLILFFFILIATFTISAVAAS-------SSAIQPVKPHIRKACRPTPYPRLCETALS 60

Query: 61  LYSSQTKTTQQELCRAAVVSSLKAAQNATSIISKLSRRKVSPYEAEVIGDCIDNLKDSIY 120
           LYSSQTK TQQELCRAAVVSSLKAAQNATSIISKLS RKVSPYEAEVIGDC DNLKDSIY
Sbjct: 61  LYSSQTKRTQQELCRAAVVSSLKAAQNATSIISKLSHRKVSPYEAEVIGDCTDNLKDSIY 120

Query: 121 ELRRATTAIKSLNRANDLKFQLSSIKTWLSAAGTDIVTCTDGLSGGAGWKVRSTVKKEVK 180
           ELRRATTAIKSLNRA+D++FQLSSIKTWLSAA T+I+TCTDGLSGGAGWKVRS VKKEVK
Sbjct: 121 ELRRATTAIKSLNRADDVEFQLSSIKTWLSAAETNIMTCTDGLSGGAGWKVRSMVKKEVK 180

Query: 181 KCSVNVIRQTSNALSLISNLKYK 203
           KCSVNVIRQTSNALSLISNLKYK
Sbjct: 181 KCSVNVIRQTSNALSLISNLKYK 196

BLAST of Csor.00g245880 vs. NCBI nr
Match: XP_008439099.1 (PREDICTED: 21 kDa protein-like [Cucumis melo])

HSP 1 Score: 289 bits (739), Expect = 1.71e-96
Identity = 152/202 (75.25%), Postives = 173/202 (85.64%), Query Frame = 0

Query: 2   KSLLILFFFILTATFTTSAVASSPSAVASSSSAIQPVKPHIKKACRPTPYPRLCETALSL 61
            SL + FFFI+  TFT       PSAVASSSS ++PVKPHI+KAC+PTPYPRLCETALSL
Sbjct: 8   SSLTLFFFFIILTTFT-------PSAVASSSSTVRPVKPHIRKACKPTPYPRLCETALSL 67

Query: 62  YSSQTKTTQQELCRAAVVSSLKAAQNATSIISKLSRRKVSPYEAEVIGDCIDNLKDSIYE 121
           Y+SQTK  QQELCRAA+VSSLKAAQNATSIISKLSRRK+S YE EVIGDCID+LKDS+ E
Sbjct: 68  YASQTKRNQQELCRAAMVSSLKAAQNATSIISKLSRRKMSAYETEVIGDCIDDLKDSVDE 127

Query: 122 LRRATTAIKSLNRANDLKFQLSSIKTWLSAAGTDIVTCTDGLSGGAGWKVRSTVKKEVKK 181
           LRRA+TAIKSL+R+ D+ FQL+SIKTW SAA TDI+TCTDGLSGG+GWKV   VKKEVK 
Sbjct: 128 LRRASTAIKSLSRSKDVDFQLNSIKTWTSAALTDIITCTDGLSGGSGWKVNKKVKKEVKN 187

Query: 182 CSVNVIRQTSNALSLISNLKYK 203
           CS+NV+RQ SNALSLI+N  YK
Sbjct: 188 CSINVVRQISNALSLINNFNYK 202

BLAST of Csor.00g245880 vs. ExPASy TrEMBL
Match: A0A6J1E4R7 (pectinesterase inhibitor 4-like OS=Cucurbita moschata OX=3662 GN=LOC111430766 PE=4 SV=1)

HSP 1 Score: 367 bits (942), Expect = 1.02e-127
Identity = 197/203 (97.04%), Postives = 201/203 (99.01%), Query Frame = 0

Query: 1   MKSLLILFFFILTATFTTSAVASSPSAVASSSSAIQPVKPHIKKACRPTPYPRLCETALS 60
           MKSLLILFFFILTATFTTSAV SSPSAV SSSSAIQPVKPHIKKACRPTPYPRLCETALS
Sbjct: 1   MKSLLILFFFILTATFTTSAVPSSPSAVPSSSSAIQPVKPHIKKACRPTPYPRLCETALS 60

Query: 61  LYSSQTKTTQQELCRAAVVSSLKAAQNATSIISKLSRRKVSPYEAEVIGDCIDNLKDSIY 120
           LYSSQTKTTQQELCRAAVVSSLKAAQNATSIISKLSRRKVSPYEAEVIGDCIDNLKDS+Y
Sbjct: 61  LYSSQTKTTQQELCRAAVVSSLKAAQNATSIISKLSRRKVSPYEAEVIGDCIDNLKDSVY 120

Query: 121 ELRRATTAIKSLNRANDLKFQLSSIKTWLSAAGTDIVTCTDGLSGGAGWKVRSTVKKEVK 180
           ELRRATTAIKSLNRAN+++FQLSSIKTWLSAAGTDIVTCTDGLSGGAGWKVRSTVKKEVK
Sbjct: 121 ELRRATTAIKSLNRANNVEFQLSSIKTWLSAAGTDIVTCTDGLSGGAGWKVRSTVKKEVK 180

Query: 181 KCSVNVIRQTSNALSLISNLKYK 203
           KCSVNVIRQTSNALSLISNLKYK
Sbjct: 181 KCSVNVIRQTSNALSLISNLKYK 203

BLAST of Csor.00g245880 vs. ExPASy TrEMBL
Match: A0A6J1J533 (pectinesterase inhibitor 4-like OS=Cucurbita maxima OX=3661 GN=LOC111483499 PE=4 SV=1)

HSP 1 Score: 332 bits (852), Expect = 4.14e-114
Identity = 182/203 (89.66%), Postives = 189/203 (93.10%), Query Frame = 0

Query: 1   MKSLLILFFFILTATFTTSAVASSPSAVASSSSAIQPVKPHIKKACRPTPYPRLCETALS 60
           MKSLLILFFFIL ATFT SAVA+S       SSAIQPVKPHI+KACRPTPYPRLCETALS
Sbjct: 1   MKSLLILFFFILIATFTISAVAAS-------SSAIQPVKPHIRKACRPTPYPRLCETALS 60

Query: 61  LYSSQTKTTQQELCRAAVVSSLKAAQNATSIISKLSRRKVSPYEAEVIGDCIDNLKDSIY 120
           LYSSQTK TQQELCRAAVVSSLKAAQNATSIISKLS RKVSPYEAEVIGDC DNLKDSIY
Sbjct: 61  LYSSQTKRTQQELCRAAVVSSLKAAQNATSIISKLSHRKVSPYEAEVIGDCTDNLKDSIY 120

Query: 121 ELRRATTAIKSLNRANDLKFQLSSIKTWLSAAGTDIVTCTDGLSGGAGWKVRSTVKKEVK 180
           ELRRATTAIKSLNRA+D++FQLSSIKTWLSAA T+I+TCTDGLSGGAGWKVRS VKKEVK
Sbjct: 121 ELRRATTAIKSLNRADDVEFQLSSIKTWLSAAETNIMTCTDGLSGGAGWKVRSMVKKEVK 180

Query: 181 KCSVNVIRQTSNALSLISNLKYK 203
           KCSVNVIRQTSNALSLISNLKYK
Sbjct: 181 KCSVNVIRQTSNALSLISNLKYK 196

BLAST of Csor.00g245880 vs. ExPASy TrEMBL
Match: A0A1S3AXZ9 (21 kDa protein-like OS=Cucumis melo OX=3656 GN=LOC103483989 PE=4 SV=1)

HSP 1 Score: 289 bits (739), Expect = 8.28e-97
Identity = 152/202 (75.25%), Postives = 173/202 (85.64%), Query Frame = 0

Query: 2   KSLLILFFFILTATFTTSAVASSPSAVASSSSAIQPVKPHIKKACRPTPYPRLCETALSL 61
            SL + FFFI+  TFT       PSAVASSSS ++PVKPHI+KAC+PTPYPRLCETALSL
Sbjct: 8   SSLTLFFFFIILTTFT-------PSAVASSSSTVRPVKPHIRKACKPTPYPRLCETALSL 67

Query: 62  YSSQTKTTQQELCRAAVVSSLKAAQNATSIISKLSRRKVSPYEAEVIGDCIDNLKDSIYE 121
           Y+SQTK  QQELCRAA+VSSLKAAQNATSIISKLSRRK+S YE EVIGDCID+LKDS+ E
Sbjct: 68  YASQTKRNQQELCRAAMVSSLKAAQNATSIISKLSRRKMSAYETEVIGDCIDDLKDSVDE 127

Query: 122 LRRATTAIKSLNRANDLKFQLSSIKTWLSAAGTDIVTCTDGLSGGAGWKVRSTVKKEVKK 181
           LRRA+TAIKSL+R+ D+ FQL+SIKTW SAA TDI+TCTDGLSGG+GWKV   VKKEVK 
Sbjct: 128 LRRASTAIKSLSRSKDVDFQLNSIKTWTSAALTDIITCTDGLSGGSGWKVNKKVKKEVKN 187

Query: 182 CSVNVIRQTSNALSLISNLKYK 203
           CS+NV+RQ SNALSLI+N  YK
Sbjct: 188 CSINVVRQISNALSLINNFNYK 202

BLAST of Csor.00g245880 vs. ExPASy TrEMBL
Match: A0A0A0L671 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G176250 PE=4 SV=1)

HSP 1 Score: 286 bits (731), Expect = 1.41e-95
Identity = 147/199 (73.87%), Postives = 174/199 (87.44%), Query Frame = 0

Query: 5   LILFFFILTATFTTSAVASSPSAVASSSSAIQPVKPHIKKACRPTPYPRLCETALSLYSS 64
           L++FFFI+  TFT       PSAV+SSSS ++PV+PHI+KAC+PTPYPRLCETALSLY+S
Sbjct: 12  LLIFFFIILTTFT-------PSAVSSSSSTVRPVQPHIRKACKPTPYPRLCETALSLYAS 71

Query: 65  QTKTTQQELCRAAVVSSLKAAQNATSIISKLSRRKVSPYEAEVIGDCIDNLKDSIYELRR 124
           QTK  QQELCRAA+VSSLKAAQNATSIISKLSRRK+S YEAEVIGDCIDNLKDS+ ELRR
Sbjct: 72  QTKRNQQELCRAAMVSSLKAAQNATSIISKLSRRKMSAYEAEVIGDCIDNLKDSVDELRR 131

Query: 125 ATTAIKSLNRANDLKFQLSSIKTWLSAAGTDIVTCTDGLSGGAGWKVRSTVKKEVKKCSV 184
           A+TAIKSL+R+ D+ FQL+SIKTW+SAA TD++TCTDGLSGG+GWKV   +KK+VK CS+
Sbjct: 132 ASTAIKSLSRSKDVDFQLNSIKTWMSAAQTDVITCTDGLSGGSGWKVSKMLKKDVKNCSI 191

Query: 185 NVIRQTSNALSLISNLKYK 203
           NV+RQ SNAL LI+N  YK
Sbjct: 192 NVVRQISNALFLINNFNYK 203

BLAST of Csor.00g245880 vs. ExPASy TrEMBL
Match: A0A6J1CJ83 (21 kDa protein-like OS=Momordica charantia OX=3673 GN=LOC111011663 PE=4 SV=1)

HSP 1 Score: 252 bits (643), Expect = 3.82e-82
Identity = 143/208 (68.75%), Postives = 167/208 (80.29%), Query Frame = 0

Query: 1   MKSLLI-LFFFILTATFTTSAVASSPSAVASSSSAIQPVKPHIKKACRPTPYPRLCETAL 60
           +KSLLI LFFF+ T    TSAVASS         A++P K HI+KAC+PTPYPRLCE +L
Sbjct: 8   LKSLLIILFFFLSTTLLPTSAVASS---------AVRPPKAHIRKACKPTPYPRLCEASL 67

Query: 61  SLYSSQTKTTQQELCRAAVVSSLKAAQNATSIISKLSRRK----VSPYEAEVIGDCIDNL 120
           SLY+S+TK TQQELCRAA+VSSLKAAQNATS IS + RR+     SPYEAEVIGDC DNL
Sbjct: 68  SLYASETKRTQQELCRAALVSSLKAAQNATSTISTIFRRRGLSRPSPYEAEVIGDCTDNL 127

Query: 121 KDSIYELRRATTAIKSLNRANDLKFQLSSIKTWLSAAGTDIVTCTDGLSGGAGWKVRSTV 180
           KDSI ELRRA+ AIKSL R  D++FQ+SSIKTW+SAA TD++TCTDG++G  G KVRS V
Sbjct: 128 KDSIDELRRASAAIKSLYRTQDVEFQISSIKTWVSAAETDVMTCTDGVNGDGGRKVRSKV 187

Query: 181 KKEVKKCSVNVIRQTSNALSLISNLKYK 203
           K EVKK ++NVIRQ SNALSLI+NLKYK
Sbjct: 188 KSEVKKSTMNVIRQISNALSLINNLKYK 206

BLAST of Csor.00g245880 vs. TAIR 10
Match: AT5G51520.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 108.6 bits (270), Expect = 5.9e-24
Identity = 74/203 (36.45%), Postives = 113/203 (55.67%), Query Frame = 0

Query: 10  FILTATFTTSAVASSPSAVASSSSAIQPVKPHIKKACRPTPYPRLCETALSLYSSQTKTT 69
           + LT  F   A  +      S+SS  Q  K  +K AC  T YP  C  +LS YSS  K+ 
Sbjct: 6   YSLTIVFVFFASTNIQKTSGSASSYSQNHKTFVKTACNSTTYPDKCYKSLSSYSSNIKSD 65

Query: 70  QQELCRAAVVSSLKAAQNATSIISKL----------SRRKVSPYEAEVIGDCIDNLKDSI 129
             +LC  A+  ++K+A+ ATS++SKL           + K+ P EA ++ DC++ +KD+I
Sbjct: 66  PIKLCTTALNLNVKSAKEATSVVSKLLKMSQKSTAGRKGKMLP-EALILKDCLEEMKDTI 125

Query: 130 YELRRATTAIKSLNRANDLKFQLSSIKTWLSAAGTDIVTCTDGLSGGAGWKVRSTVKKEV 189
            EL++A T +K+L     +   +++++TW+S+A TD  TCTDG       KV    KK+V
Sbjct: 126 IELKQAITEMKNLQDGGSMAEHITNVRTWVSSALTDEGTCTDGFE---EVKVNKETKKKV 185

Query: 190 KKCSVNVIRQTSNALSLISNLKY 203
            K    +   TSN L+LI+NL+Y
Sbjct: 186 NKVVEELATTTSNTLALITNLRY 204

BLAST of Csor.00g245880 vs. TAIR 10
Match: AT4G25250.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 97.4 bits (241), Expect = 1.4e-20
Identity = 66/198 (33.33%), Postives = 109/198 (55.05%), Query Frame = 0

Query: 6   ILFFFILTATFTTSAVASS-PSAVASSSSAIQPVKPHIKKACRPTPYPRLCETALSLYSS 65
           +L F +L+ T      +S+ P   A+     Q    ++K AC  T YP +C   LS YSS
Sbjct: 1   MLRFVVLSLTLMVFINSSNFPKTAATPPGTYQNHTTYVKTACNSTTYPTMCYNCLSSYSS 60

Query: 66  QTKTTQQELCRAAVVSSLKAAQNATSIISKL--SRRKVSPYEAEVIGDCIDNLKDSIYEL 125
             K+   +LC  ++  ++K+A+NAT ++S L    +    +E  ++ DC+D +KD+I EL
Sbjct: 61  TIKSDPIKLCTTSLNLNVKSAKNATLVVSNLLQKAKAAKSHEVSILKDCVDEMKDTIDEL 120

Query: 126 RRATTAIKSL-NRANDLKFQLSSIKTWLSAAGTDIVTCTDGLSGGAGWKVRSTVKKEVKK 185
           ++A   +K +       +  L ++KTW+S+A TD  TCTDG   G   +V    KK+VKK
Sbjct: 121 KQAVAEMKYVRGGGKTTEEHLKNVKTWVSSALTDEGTCTDGFEEG---RVNVETKKKVKK 180

Query: 186 CSVNVIRQTSNALSLISN 200
               + + TSN L+L+++
Sbjct: 181 AISELSKTTSNTLALLTH 195

BLAST of Csor.00g245880 vs. TAIR 10
Match: AT5G62350.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 91.3 bits (225), Expect = 9.8e-19
Identity = 61/195 (31.28%), Postives = 106/195 (54.36%), Query Frame = 0

Query: 8   FFFILTATFTTSAVASSPSAVASSSSAIQPVKPHIKKACRPTPYPRLCETALSLYSSQTK 67
           + F+L +     ++  + +  AS + A +     I+ +C+ T YP LC  +LS+Y++  +
Sbjct: 5   YLFVLLSISYLLSLELTAATAASQTGASKKAINFIQSSCKTTTYPALCVHSLSVYANDIQ 64

Query: 68  TTQQELCRAAVVSSLKAAQNATSIISKLSRRK-VSPYEAEVIGDCIDNLKDSIYELRRAT 127
           T+ + L   A+  +L  AQ+    +S+L+R K +   E E I DC++ + D++  L ++ 
Sbjct: 65  TSPKRLAETAIAVTLSRAQSTKLFVSRLTRMKGLKKREVEAIKDCVEEMNDTVDRLTKSV 124

Query: 128 TAIKSLNRAND---LKFQLSSIKTWLSAAGTDIVTCTDGLSGGAGWKVRSTVKKEVKKCS 187
             +K    A D     + +S+ +TW SAA TD  TC+DG SG     +   +K  V+   
Sbjct: 125 QELKLCGSAKDQDQFAYHMSNAQTWTSAALTDENTCSDGFSGRV---MDGRIKNSVRARI 184

Query: 188 VNVIRQTSNALSLIS 199
           +NV  +TSNALSLI+
Sbjct: 185 MNVGHETSNALSLIN 196

BLAST of Csor.00g245880 vs. TAIR 10
Match: AT5G62360.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 87.8 bits (216), Expect = 1.1e-17
Identity = 54/158 (34.18%), Postives = 97/158 (61.39%), Query Frame = 0

Query: 42  IKKACRPTPYPRLCETALSLYSSQTKTTQQELCRAAVVSSLKAAQNATSIISKLSRRKVS 101
           +K +C  T YPRLC ++LS ++S  +T+ + +  AA+  +L +A+  ++++ +LS  ++ 
Sbjct: 44  VKSSCTFTTYPRLCFSSLSTHASLIQTSPKLMAHAALNITLASAKVTSAMMVRLSNSRLK 103

Query: 102 PYEAEVIGDCIDNLKDSIYELRRATTAIKSLNRANDLKFQLSSIKTWLSAAGTDIVTCTD 161
           P E   + DC++ L D++ ELR++   +  L+ +N  +  +S I+TW+SAA TD+ TCTD
Sbjct: 104 PKEVSAMRDCVEELGDTLEELRKSIGEMCQLSGSN-YEVYISDIQTWVSAALTDVNTCTD 163

Query: 162 GLSGGAGWKVRSTVKKEVKKCSVNVIRQTSNALSLISN 200
           G  G     +   VK  V+   + +   TSNAL+LI++
Sbjct: 164 GFEGE---DMDGKVKVLVRGRILVIAHLTSNALALINH 197

BLAST of Csor.00g245880 vs. TAIR 10
Match: AT1G62770.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 81.6 bits (200), Expect = 7.8e-16
Identity = 69/206 (33.50%), Postives = 112/206 (54.37%), Query Frame = 0

Query: 9   FFILTATFTTSAVASSPSAVASSSSAIQPVKPHIKKACRPTPYPRLCETALSLYSSQTK- 68
           F ++  + T   +  S SA  + S + Q     I  +C+ T YP LC   LS Y+++ + 
Sbjct: 8   FLVILLSIT---ILQSSSATPNRSESDQ----FIVSSCQTTQYPSLCVHTLSAYATKIRH 67

Query: 69  TTQQELCRAAVVSSLKAAQNATSIISKLSRR----KVSPYEA-----EVIGDCIDNLKDS 128
              Q+L + A++ SL  A++ T  ++KL++     K   Y A     EV+G+ +D L  S
Sbjct: 68  NNDQDLAQTALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQS 127

Query: 129 IYELRRATTAIKSLNRANDLKFQLSSIKTWLSAAGTDIVTCTDGLSGGA-GWKVRSTVKK 188
           + EL RA  A+ S     D  +++S+++TW+SAA TD  TC DG S  A G KV+  ++ 
Sbjct: 128 VKELARAGHAVAS----EDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRY 187

Query: 189 EVKKCSVNVIRQTSNALSLISNLKYK 204
           +V    V+V + TSNAL+L++    K
Sbjct: 188 KV----VHVAQVTSNALALVNQFAEK 198

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P174076.6e-2034.3921 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1[more]
Q9SB381.9e-1933.33Pectinesterase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=PMEI4 PE=2 SV=1[more]
Q84WE41.1e-1434.13Pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PMEI3 PE=2 SV=1[more]
Q9SI721.1e-1433.50Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1[more]
Q9SI741.4e-1431.35Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
KAG6576794.16.56e-131100.00hypothetical protein SDJN03_24368, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022922937.12.10e-12797.04pectinesterase inhibitor 4-like [Cucurbita moschata][more]
XP_023553135.18.20e-12595.5721 kDa protein-like [Cucurbita pepo subsp. pepo][more]
XP_022985512.18.55e-11489.66pectinesterase inhibitor 4-like [Cucurbita maxima][more]
XP_008439099.11.71e-9675.25PREDICTED: 21 kDa protein-like [Cucumis melo][more]
Match NameE-valueIdentityDescription
A0A6J1E4R71.02e-12797.04pectinesterase inhibitor 4-like OS=Cucurbita moschata OX=3662 GN=LOC111430766 PE... [more]
A0A6J1J5334.14e-11489.66pectinesterase inhibitor 4-like OS=Cucurbita maxima OX=3661 GN=LOC111483499 PE=4... [more]
A0A1S3AXZ98.28e-9775.2521 kDa protein-like OS=Cucumis melo OX=3656 GN=LOC103483989 PE=4 SV=1[more]
A0A0A0L6711.41e-9573.87PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G176250 PE=4 S... [more]
A0A6J1CJ833.82e-8268.7521 kDa protein-like OS=Momordica charantia OX=3673 GN=LOC111011663 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G51520.15.9e-2436.45Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
AT4G25250.11.4e-2033.33Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
AT5G62350.19.8e-1931.28Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
AT5G62360.11.1e-1734.18Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
AT1G62770.17.8e-1633.50Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 37..196
e-value: 5.6E-36
score: 135.5
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 1..200
e-value: 1.0E-29
score: 101.1
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 42..196
e-value: 9.1E-29
score: 100.7
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 39..202
e-value: 2.4E-32
score: 114.0
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 40..200
NoneNo IPR availablePANTHERPTHR31080:SF15PECTINESTERASE INHIBITOR 4coord: 8..200
NoneNo IPR availablePANTHERPTHR31080PECTINESTERASE INHIBITOR-LIKEcoord: 8..200
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 45..200
e-value: 1.38162E-37
score: 126.014

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g245880.m01Csor.00g245880.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043086 negative regulation of catalytic activity
molecular_function GO:0004857 enzyme inhibitor activity